ENVIS CENTER on ENVIRONMENTAL BIO TECHNOLOGY
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Exploring Energy Gardens Botanic Gardens and Biofuels Volume 11 • Number 1 EDITORIAL BIOENERGY and the ROLE of BOTANIC GARDENS Sara Oldfield
Journal of Botanic Gardens Conservation International Volume 11 • Number 1 • January 2014 Exploring energy gardens Botanic gardens and biofuels Volume 11 • Number 1 EDITORIAL BIOENERGY AND THE ROLE OF BOTANIC GARDENS Sara Oldfield .... 02 EDITORS NEPAL ENERGY GARDEN PROJECT: EXPLORING ENERGY GARDENS AS A SOURCE FOR LOCAL FUEL PRODUCTION Jon Lovett .... 03 BIOENERGY DAY @ UGA Terry Marie Hastings .... 07 WASTE-TO-ENERGY: CONSERVING PLANT GENETIC RESOURCES, IMPROVING LIVELIHOODS David K. Nkwanga .... 11 ENALGAE PILOT AT THE CAMBRIDGE UNIVERSITY BOTANIC GARDEN Suzanne Sharrock Sara Oldfield A CASE STUDY OF BIO-ENGINEERING WORK Beatrix Schlarb-Ridley .... 14 Director of Global Secretary General Programmes BIOENERGY PLANT COLLECTION AND RESEARCH IN THE XISHUANGBANNA TROPICAL BOTANICAL GARDEN Cover Photo : Child carrying firewood in Gorkha, Nepal .... 16 Zzvet/Shutterstock.com Zeng-Fu Xu, Jianxiang Hu, Tianping Huang, and Jin Chen Design : Seascape www.seascapedesign.co.uk DESIGNING AN ENERGY GARDEN Trudi Entwistle .... 19 BGjournal is published by Botanic Gardens Conservation International (BGCI) . It is published twice a year. HASSAN BIOFUEL PARK: A CONCEPT FOR PROMOTION OF Membership is open to all interested individuals, institutions and organisations that support the aims REPLENISHABLE GREEN ENERGY Balakrishna Gowda, K.T. .... 23 of BGCI. Prasanna, G.C. Vijaya Kumar, C. Haleshi and K. Rajesh Kumar Further details available from: • Botanic Gardens Conservation International, Descanso RESOURCES House, 199 Kew Road, Richmond, Surrey TW9 3BW -
Canada – 2018 Update
Canada – 2018 update Bioenergy policies and status of implementation Country Reports IEA Bioenergy: 09 2018 This report was prepared from the 2018 OECD/IEA World Energy Balances, combined with data and information provided by the IEA Bioenergy Executive Committee and Task members. All individual country reports were reviewed by the national delegates to the IEA Bioenergy Executive Committee, who have approved the content. General background on the approach and definitions can be found in the central introductory report1 for all country reports. Edited by: Luc Pelkmans, Technical Coordinator IEA Bioenergy Contributors: Alex MacLeod, Bruno Gagnon, Devin O’Grady, Yana Mazin and Maria Wellisch NATIONAL POLICY FRAMEWORK IN CANADA In 2015, at the United Nations Climate Conference (COP21) in Paris, Canada together with 194 other participating countries, agreed to take steps to support the transition to a global low-carbon economy that would limit the global temperature increase to less than 2 degrees Celsius above pre-industrial levels. Canada’s government at both federal and provincial and territorial levels is committing to policies such as supporting renewable/clean technology innovation as announced in the Federal Budgets 20162, and 20173. Furthermore, to support the COP21 commitment, the federal government in partnership with provinces and territories, and in consultation with Indigenous peoples has developed a pan-Canadian Framework on Clean Growth and Climate Change4. It includes a federal carbon pricing framework and measures to achieve reductions across all sectors of the economy. The Pan-Canadian Approach to Pricing Carbon Pollution was announced October 3, 2016. On November 25, 2016, the Government of Canada announced its intent to develop a Clean Fuel Standard (CFS). -
Bacterial Degradation of Isoprene in the Terrestrial Environment Myriam
Bacterial Degradation of Isoprene in the Terrestrial Environment Myriam El Khawand A thesis submitted to the University of East Anglia in fulfillment of the requirements for the degree of Doctor of Philosophy School of Environmental Sciences November 2014 ©This copy of the thesis has been supplied on condition that anyone who consults it is understood to recognise that its copyright rests with the author and that use of any information derive there from must be in accordance with current UK Copyright Law. In addition, any quotation or extract must include full attribution. Abstract Isoprene is a climate active gas emitted from natural and anthropogenic sources in quantities equivalent to the global methane flux to the atmosphere. 90 % of the emitted isoprene is produced enzymatically in the chloroplast of terrestrial plants from dimethylallyl pyrophosphate via the methylerythritol pathway. The main role of isoprene emission by plants is to reduce the damage caused by heat stress through stabilizing cellular membranes. Isoprene emission from microbes, animals, and humans has also been reported, albeit less understood than isoprene emission from plants. Despite large emissions, isoprene is present at low concentrations in the atmosphere due to its rapid reactions with other atmospheric components, such as hydroxyl radicals. Isoprene can extend the lifetime of potent greenhouse gases, influence the tropospheric concentrations of ozone, and induce the formation of secondary organic aerosols. While substantial knowledge exists about isoprene production and atmospheric chemistry, our knowledge of isoprene sinks is limited. Soils consume isoprene at a high rate and contain numerous isoprene-utilizing bacteria. However, Rhodococcus sp. AD45 is the only terrestrial isoprene-degrading bacterium characterized in any detail. -
429 Chem. Listy 97, 363–520 (2003)
Chem. Listy 97, 363–520 (2003) Posters P117 APPLICATION OF DEGENERATE REFERENCES OLIGONUCLEOTIDE GENE SHUFFLING FOR CONSTRUCTION OF HYBRID 1. Janssen D. B., Pries F., Ploeg J., Kazemier B., Terpstra P., HALOALKANE DEHALOGENASE Witholt B.: J. Bacteriol. 171, 6791 (1989). 2. Nagata Y., Nariya T., Ohtomo R., Fukuda M., Yano K., ANDREA JESENSKÁa, YUJI NAGATAb, Takagi M.: J. Bacteriol. 175, 6403 (1993). and JIŘÍ DAMBORSKÝc 3. Kulakova A. N., Larkin M. J., Kulakov L. A.: Microbio- logy 143, 109 (1997). a National Centre for Biomolecular Research, Faculty of Science, 4. Jesenska A., Bartos M., Czernekova V., Rychlik I., Masaryk University, Kotlářská 2, Brno, 611 37, Czech Republic, Pavlik I., Damborsky V.: Appl. Environ. Microbiol. 68, e-mail: [email protected]; bDepartment of Environmen- 3724 (2002). tal Life Sciences, Graduate School of Life Sciences, Tohoku 5. Gibbs M. D., Nevalainen K. M., Bergquist P. L.: Gene University, Sendai 980-8577, Japan 271, 13 (2001). Keywords: haloalkane dehalogenase, in vitro recombination, P118 KINETICS AND SPECIFICITY construct, substrate specificity OF HALOALKANE DEHALOGENASE LinB FROM Sphingomonas paucimobilis UT26 Halogenated organic compounds constitute one of the ZBYNĚK PROKOPa, MARTA MONINCOVÁa, largest groups of environmental pollutants as a result of their MARTIN KLVAŇAa, RADKA CHALOUPKOVÁa, widespread use in industry and agriculture. Haloalkane de- DICK B. JANSSENb, YUJI NAGATAc, halogenases hydrolytically convert halogenated aliphatic and JIŘÍ DAMBORSKÝa compounds to corresponding alcohols. Enhancement of cata- lytic properties of environmentally important enzymes using aNational Centre for Biomolecular Research, Masaryk Univer- in vitro evolution techniques is one of the obvious goal of bio- sity, Kotlářská 2, 611 37 Brno, Czech Republic; bUniversity of technology. -
Genetic Characterisaton of Rhodococcus Rhodochrous ATCC
The copyright of this thesis vests in the author. No quotation from it or information derived from it is to be published without full acknowledgement of the source. The thesis is to be used for private study or non- commercial research purposes only. Published by the University of Cape Town (UCT) in terms of the non-exclusive license granted to UCT by the author. University of Cape Town Genetic characterization of Rhodococcus rhodochrous ATCC BAA-870 with emphasis on nitrile hydrolysing enzymes n ow Joni Frederick A thesis submitted in fulfilment of the requirements for the degree of Doctor of Philosophy in the Departmentty of of MolecularCape and T Cell Biology, Universitysi of Cape Town er UnivSupervisor: Professor B. T. Sewell Co-supervisor: Professor D. Brady February 2013 Keywords Nitrile hydrolysis Biocatalysis Rhodococcus rhodochrous ATCC BAA-870 Genome sequencing Nitrilase Nitrile hydratase n ow ty of Cape T si er Univ ii Keywords Abstract Rhodococcus rhodochrous ATCC BAA-870 (BAA-870) had previously been isolated on selective media for enrichment of nitrile hydrolysing bacteria. The organism was found to have a wide substrate range, with activity against aliphatics, aromatics, and aryl aliphatics, and enantioselectivity towards beta substituted nitriles and beta amino nitriles, compounds that have potential applications in the pharmaceutical industry. This makes R. rhodochrous ATCC BAA-870 potentially a versatile biocatalyst for the synthesis of a broad range of compounds with amide and carboxylic acid groups that can be derived from structurally related nitrile precursors. The selectivity of biocatalysts allows for high product yields and better atom economyn than non- selective chemical methods of performing this reaction, suchow as acid or base hydrolysis. -
Advanced Development and Optimization – Integration And
2017 PROJECT PEER REVIEW ADVANCED DEVELOPMENT AND OPTIMIZATION: INTEGRATION AND SCALE-UP TECHNOLOGY AREA 27 Advanced Development and Optimization: Integration and Scale-up 2019 PROJECT PEER REVIEW CONTENTS INTRODUCTION ........................................................................................................................................ 766 ADO: INTEGRATION AND SCALE-UP OVERVIEW ..................................................................................... 766 ADO: INTEGRATION AND SCALE-UP REVIEW PANEL ............................................................................. 767 TECHNOLOGY AREA SCORE RESULTS ................................................................................................... 768 ADO: INTEGRATION AND SCALE-UP REVIEW PANEL SUMMARY REPORT ............................................ 769 ADO: INTEGRATION AND SCALE-UP PROGRAMMATIC RESPONSE ....................................................... 772 BIOMASS – FEEDSTOCK USER FACILITY ............................................................................................... 775 HYDROTHERMAL PROCESSING OF BIOMASS ....................................................................................... 779 PILOT-SCALE INTEGRATION .................................................................................................................... 782 INTEGRATION AND SCALE-UP ................................................................................................................. 785 BIOMASS GASIFICATION FOR CHEMICALS PRODUCTION -
12) United States Patent (10
US007635572B2 (12) UnitedO States Patent (10) Patent No.: US 7,635,572 B2 Zhou et al. (45) Date of Patent: Dec. 22, 2009 (54) METHODS FOR CONDUCTING ASSAYS FOR 5,506,121 A 4/1996 Skerra et al. ENZYME ACTIVITY ON PROTEIN 5,510,270 A 4/1996 Fodor et al. MICROARRAYS 5,512,492 A 4/1996 Herron et al. 5,516,635 A 5/1996 Ekins et al. (75) Inventors: Fang X. Zhou, New Haven, CT (US); 5,532,128 A 7/1996 Eggers Barry Schweitzer, Cheshire, CT (US) 5,538,897 A 7/1996 Yates, III et al. s s 5,541,070 A 7/1996 Kauvar (73) Assignee: Life Technologies Corporation, .. S.E. al Carlsbad, CA (US) 5,585,069 A 12/1996 Zanzucchi et al. 5,585,639 A 12/1996 Dorsel et al. (*) Notice: Subject to any disclaimer, the term of this 5,593,838 A 1/1997 Zanzucchi et al. patent is extended or adjusted under 35 5,605,662 A 2f1997 Heller et al. U.S.C. 154(b) by 0 days. 5,620,850 A 4/1997 Bamdad et al. 5,624,711 A 4/1997 Sundberg et al. (21) Appl. No.: 10/865,431 5,627,369 A 5/1997 Vestal et al. 5,629,213 A 5/1997 Kornguth et al. (22) Filed: Jun. 9, 2004 (Continued) (65) Prior Publication Data FOREIGN PATENT DOCUMENTS US 2005/O118665 A1 Jun. 2, 2005 EP 596421 10, 1993 EP 0619321 12/1994 (51) Int. Cl. EP O664452 7, 1995 CI2O 1/50 (2006.01) EP O818467 1, 1998 (52) U.S. -
Microbial Cycling of Isoprene, the Most Abundantly Produced Biological Volatile Organic Compound on Earth
The ISME Journal (2018) 12:931–941 https://doi.org/10.1038/s41396-018-0072-6 REVIEW ARTICLE Microbial cycling of isoprene, the most abundantly produced biological volatile organic compound on Earth 1 2 3 Terry J. McGenity ● Andrew T. Crombie ● J. Colin Murrell Received: 30 June 2017 / Revised: 26 September 2017 / Accepted: 12 December 2017 / Published online: 20 February 2018 © The Author(s) 2018. This article is published with open access Abstract Isoprene (2-methyl-1,3-butadiene), the most abundantly produced biogenic volatile organic compound (BVOC) on Earth, is highly reactive and can have diverse and often detrimental atmospheric effects, which impact on climate and health. Most isoprene is produced by terrestrial plants, but (micro)algal production is important in aquatic environments, and the relative bacterial contribution remains unknown. Soils are a sink for isoprene, and bacteria that can use isoprene as a carbon and energy source have been cultivated and also identified using cultivation-independent methods from soils, leaves and coastal/ marine environments. Bacteria belonging to the Actinobacteria are most frequently isolated and identified, and Proteobacteria have also been shown to degrade isoprene. In the freshwater-sediment isolate, Rhodococcus strain AD45, 1234567890();,: initial oxidation of isoprene to 1,2-epoxy-isoprene is catalyzed by a multicomponent isoprene monooxygenase encoded by the genes isoABCDEF. The resultant epoxide is converted to a glutathione conjugate by a glutathione S-transferase encoded by isoI, and further degraded by enzymes encoded by isoGHJ. Genome sequence analysis of actinobacterial isolates belonging to the genera Rhodococcus, Mycobacterium and Gordonia has revealed that isoABCDEF and isoGHIJ are linked in an operon, either on a plasmid or the chromosome. -
UNCONVENTIONAL BIOMASS PRODUCTION Chapter 4.— UNCONVENTIONAL BIOMASS PRODUCTION
Chapter 4 UNCONVENTIONAL BIOMASS PRODUCTION Chapter 4.— UNCONVENTIONAL BIOMASS PRODUCTION Page Introduction . 91 TABLES Genetics . 91 Page Crop Yields .*. ... ... ... ... 92 36. Optimistic Future Average Crop Yields for Unconventional land-Based Crops. 95 Plants Under Large-Scale Production . 94 Lignocellulose Crops . 95 37. incomplete List of Candidate Vegetable Oil and Hydrocarbon Crops . 96 Unconventional Bioenergy Crops . 95 Starch and Sugar Crops . 97 38. Optimistic Cost Estimates for General Aspects . 97 Unconventional Crops. 97 Aquiculture . 98 Mariculture ● ..********...........*.***** 101 Other Unconventional Approaches . ..........105 Multiple Cropping . .105 Chemical Inoculation. .................105 Energy Farms . .. ...105 FIGURE Biophotolysis. ....106 Inducing Nitrogen Fixation in Plants . ......107 Page Greenhouses . .. ...108 13. Macrocystis Pyrifera . .................102 Chapter 4 UNCONVENTIONAL BIOMASS PRODUCTION Introduction A number of unconventional approaches to istics, however, can aid in comparing the vari- biomass energy production have been pro- ous possible types of energy crops. posed. Several nontraditional crops that pro- The general aspects of farming, plant duce vegetable oils, hydrocarbons, and other growth, and the efficiency of photosynthesis chemicals or cellulosic material are under in- are considered in chapter 3. Since future crop vestigation. Both freshwater and saltwater yields will depend on these factors and on the plants are being considered, and various other development of hybrids for energy production, approaches to biomass fuel production are the possibilities for genetic improvements are being examined. A common feature to all of considered here. Following this, crop yields these approaches is that the full potential of and various unconventional bioenergy crops individual plants proposed as fuel-producers and approaches to farming them are discussed. cannot be fully assessed without further R&D. -
Dissertation
Dissertation Homo- and Heterobimetallic Complexes of the PDIxCy ligand system; Synthesis, Characterization and Reactivity Andreas Joseph Hofmann Technische Universität München Fachgebiet Bioanorganische Chemie Homo- and Heterobimetallic Complexes of the PDIxCy ligand system; Synthesis, Characterization and Reactivity Andreas Joseph Hofmann Vollständiger Abdruck der von der Fakultät Chemie der Technischen Universität München zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) genehmigten Dissertation. Vorsitzende(r): Prof. Dr. Roland A. Fischer Prüfer der Dissertation: 1. Prof. Dr. Corinna R. Hess 2. Hon.-Prof. Dr. Richard W. Fischer Die Dissertation wurde am 15.02.2021 bei der Technischen Universität München eingereicht und durch die Fakultät für Chemie am 30.03.2021 angenommen. Die vorliegende Arbeit wurde im Fachgebiet Bioanorganische Chemie der Technischen Universität München in der Zeit von Juni 2017 bis Februar 2021 angefertigt. Teile dieser Arbeit wurden bereits veröffentlicht: Andreas J. Hofmann, Christian Jandl, Corinna R. Hess “Structural Differences and Redox Properties of Unsymmetric Diiron PDIxCy Complexes” European Journal of Inorganic Chemistry 2020, 2020, 499-505. Andreas J. Hofmann, Lukas Niederegger, Corinna R. Hess “Neighbouring effects on catalytic epoxidation by Fe-cyclam in M2-PDIxCy complexes” Dalton Transactions 2020, 49, 17642-17648. Mein besonderer Dank gilt meiner Doktormutter Frau Prof. Dr. Corinna R. Hess für die Aufnahme in ihren Arbeitskreis und die Möglichkeit, an dieser interessanten Themenstellung zu arbeiten. Außerdem möchte ich Frau Prof. Hess für die wissenschaftliche Betreuung und das mir entgegengebrachte Vertrauen danken. 1.1 Danksagung Mein Dank gilt: Lukas Niederegger, der mich in der ganzen Zeit meiner Promotion unterstützt hat, für seine hilfreichen Tipps und sein großes Engagement, das er für jedes Thema aufbrachte. -
Building up the Future Cost of Biofuel Sub Group on Advanced Biofuels
Building Up the Future Cost of Biofuel Sub Group on Advanced Biofuels Sustainable Transport Forum Edited by: Kyriakos Maniatis Ingvar Landälv Lars Waldheim Eric van den Heuvel Stamatis Kalligeros March – 2017 Disclaimer: The SGAB report has been approved by the Members of the Sustainable Transport Forum. However, a Member has expressed its concern regarding the proposed recommendations on food-based biofuels. This Member also considered that the report should give deeper consideration to the sustainable availability of lipid-based advanced biofuels. EUROPEAN COMMISSION Directorate-General for Transport Unit MOVE.DDG1.B.4 - Sustainable & Intelligent Transport Directorate B - Investment, Innovative & Sustainable Transport Contact: Antonio Tricas-Aizpun, E-mail: [email protected] European Commission B-1049 Brussels iii Europe Direct is a service to help you find answers to your questions about the European Union. Freephone number (*): 00 800 6 7 8 9 10 11 (*) The information given is free, as are most calls (though some operators, phone boxes or hotels may charge you). LEGAL NOTICE This document has been prepared for the European Commission however it reflects the views only of the authors, and the Commission cannot be held responsible for any use which may be made of the information contained therein. More information on the European Union is available on the Internet (http://www.europa.eu). Luxembourg: Publications Office of the European Union, 2018 ISBN 978-92-79-73178-5 doi: 10.2832/163774 © European Union, 2018 Reproduction -
Rekombinante Herstellung Und Charakterisierung Phenoloxidierender Enzyme Aus Geobacillus Stearothermophilus Zur Evaluierung Einer Biosensorischen Anwendung
Rekombinante Herstellung und Charakterisierung phenoloxidierender Enzyme aus Geobacillus stearothermophilus zur Evaluierung einer biosensorischen Anwendung Von der Fakultät für Mathematik, Informatik und Naturwissenschaften der Rheinisch- Westfälischen Technischen Hochschule Aachen zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften genehmigte Dissertation vorgelegt von Diplom-Biologe Jäntges Uwe Konrad aus Aachen Berichter: Universitätsprofessor Dr.-Ing. Winfried Hartmeier Universitätsprofessor Dr. rer. nat. Andreas Schäffer Tag der mündlichen Prüfung: 10.02.2006 Diese Dissertation ist auf den Internetseiten der Hochschulbibliothek online verfügbar Für meine Eltern: Clemens und Marlene Jäntges Inhaltsverzeichnis 1 EINLEITUNG 1 1.1 Phenol 1 1.2 Enzymatische Umwandlung und biosensorische Anwendung 3 1.3 Phenolhydroxylasen 5 1.4 Phenoloxidierende Enzyme aus G. stearothermophilus 7 1.5 Rekombinante Expression von Enzymen 9 1.6 Immobilisierung 10 1.7 Aufgabenstellung 11 2 MATERIAL UND METHODEN 13 2.1 Material 13 2.1.1 Chemikalien 13 2.1.2 Enzyme, Reaktionskits und Größenmarker 13 2.1.3 Geräte, Software und Datenbanken 13 2.1.4 Mikroorganismen 15 2.1.5 Vektoren 16 2.1.6 Synthetische Oligonukleotide 16 2.1.7 Rekombinante Plasmide 17 2.1.8 Puffer 17 2.2 Analytik 18 2.2.1 Phenolanalytik 18 2.2.2 Messung von gelöstem Sauerstoff 19 2.3 Mikrobiologische Methoden 20 2.3.1 Anlegen von Stammkulturen 20 2.3.2 Kultivierung 20 2.4 Molekularbiologische Methoden 22 2.4.1 Isolation genomischer DNA aus G. stearothermophilus 22 2.4.2 Agarose-Gelelektrophorese von DNA-Molekülen 23 2.4.3 Polymerasekettenreaktion 23 2.4.4 Restriktionsverdau von DNA 23 2.4.5 Extraktion von DNA-Fragmenten aus Agarosegelen 23 2.4.6 Ligation von DNA 24 2.4.7 Isolation von Plasmid-DNA aus E.