Supplemental Table 1A. Differential Gene Expression Profile of Adehcd40l and Adehnull Treated Cells Vs Untreated Cells

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Supplemental Table 1A. Differential Gene Expression Profile of Adehcd40l and Adehnull Treated Cells Vs Untreated Cells Supplemental Table 1a. Differential Gene Expression Profile of AdEHCD40L and AdEHNull treated cells vs Untreated Cells Fold change Regulation Fold change Regulation ([AdEHCD40L] vs ([AdEHCD40L] ([AdEHNull] vs ([AdEHNull] vs Probe Set ID [Untreated]) vs [Untreated]) [Untreated]) [Untreated]) Gene Symbol Gene Title RefSeq Transcript ID NM_001039468 /// NM_001039469 /// NM_004954 /// 203942_s_at 2.02 down 1.00 down MARK2 MAP/microtubule affinity-regulating kinase 2 NM_017490 217985_s_at 2.09 down 1.00 down BAZ1A fibroblastbromodomain growth adjacent factor receptorto zinc finger 2 (bacteria-expressed domain, 1A kinase, keratinocyte NM_013448 /// NM_182648 growth factor receptor, craniofacial dysostosis 1, Crouzon syndrome, Pfeiffer 203638_s_at 2.10 down 1.01 down FGFR2 syndrome, Jackson-Weiss syndrome) NM_000141 /// NM_022970 1570445_a_at 2.07 down 1.01 down LOC643201 hypothetical protein LOC643201 XM_001716444 /// XM_001717933 /// XM_932161 231763_at 3.05 down 1.02 down POLR3A polymerase (RNA) III (DNA directed) polypeptide A, 155kDa NM_007055 1555368_x_at 2.08 down 1.04 down ZNF479 zinc finger protein 479 NM_033273 /// XM_001714591 /// XM_001719979 241627_x_at 2.15 down 1.05 down FLJ10357 hypothetical protein FLJ10357 NM_018071 223208_at 2.17 down 1.06 down KCTD10 potassium channel tetramerisation domain containing 10 NM_031954 219923_at 2.09 down 1.07 down TRIM45 tripartite motif-containing 45 NM_025188 242772_x_at 2.03 down 1.07 down Transcribed locus 233019_at 2.19 down 1.08 down CNOT7 CCR4-NOT transcription complex, subunit 7 NM_013354 /// NM_054026 223139_s_at 2.09 down 1.08 down DHX36 DEAH (Asp-Glu-Ala-His) box polypeptide 36 NM_001114397 /// NM_020865 1569594_a_at 2.17 down 1.09 down SDCCAG1 serologically defined colon cancer antigen 1 NM_004713 238518_x_at 2.13 down 1.09 down GLYCTK glycerate kinase NM_145262 217650_x_at 2.83 down 1.09 down ST3GAL2 ST3 beta-galactoside alpha-2,3-sialyltransferase 2 NM_006927 218862_at 2.12 down 1.13 down ASB13 ankyrin repeat and SOCS box-containing 13 NM_024701 215587_x_at 2.83 down 1.13 down CDNA FLJ13829 fis, clone THYRO1000625 215623_x_at 2.33 down 1.13 down SMC4 structural maintenance of chromosomes 4 NM_001002800 /// NM_005496 231479_at 2.00 down 1.14 down TTC33 tetratricopeptide repeat domain 33 NM_012382 222854_s_at 2.05 down 1.14 down GEMIN8 gem (nuclear organelle) associated protein 8 NM_001042479 /// NM_001042480 /// NM_017856 242766_at 2.13 down 1.14 down Homo sapiens, clone IMAGE:3660074, mRNA 228766_at 2.45 down 1.15 down 200598_s_at 2.11 down 1.16 down HSP90B1 heat shock protein 90kDa beta (Grp94), member 1 NM_003299 221755_at 2.10 down 1.17 down EHBP1L1 EH domain binding protein 1-like 1 NM_001099409 1556082_a_at 2.20 down 1.18 down CDNA FLJ33441 fis, clone BRACE2021932 205099_s_at 2.01 down 1.18 down CCR1 chemokine (C-C motif) receptor 1 NM_001295 203731_s_at 2.00 down 1.18 down ZKSCAN5 zinc finger with KRAB and SCAN domains 5 NM_014569 /// NM_145102 233511_at 2.07 down 1.18 down CDNA clone IMAGE:5271538 235985_at 2.89 down 1.18 down Transcribed locus 214987_at 2.41 down 1.19 down CDNA clone IMAGE:4801326 230888_at 2.18 down 1.19 down WDR91 WD repeat domain 91 NM_014149 225272_at 2.04 down 1.20 down SAT2 spermidine/spermine N1-acetyltransferase family member 2 NM_133491 201491_at 2.02 down 1.20 down AHSA1 AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast) NM_012111 205240_at 2.74 down 1.20 down GPSM2 G-protein signaling modulator 2 (AGS3-like, C. elegans) NM_013296 229466_at 2.14 down 1.21 down LOC256273 hypothetical protein LOC256273 227507_at 2.06 down 1.22 down ZNF592 zinc finger protein 592 NM_014630 225377_at 2.21 down 1.22 down C9orf86 chromosome 9 open reading frame 86 NM_017995 /// NM_024718 212601_at 2.47 down 1.22 down ZZEF1 zinc finger, ZZ-type with EF-hand domain 1 NM_015113 228789_at 2.92 down 1.24 down MTMR6 myotubularin related protein 6 NM_004685 205854_at 2.44 down 1.24 down TULP3 tubby like protein 3 NM_003324 214836_x_at 2.04 down 1.26 down IGKC immunoglobulin kappa constant 218895_at 2.82 down 1.26 down GPATCH3 G patch domain containing 3 NM_022078 215521_at 2.16 down 1.27 down PHC3 polyhomeotic homolog 3 (Drosophila) NM_024947 204492_at 2.26 down 1.27 down ARHGAP11A Rho GTPase activating protein 11A NM_014783 /// NM_199357 /// XM_001714495 221436_s_at 2.14 down 1.28 down CDCA3 cell division cycle associated 3 NM_031299 224736_at 2.63 down 1.28 down CCAR1 cell division cycle and apoptosis regulator 1 NM_018237 214965_at 2.17 down 1.29 down SPATA2L spermatogenesis associated 2-like NM_152339 235084_x_at 2.56 down 1.30 down Transcribed locus 244792_at 2.06 down 1.31 down 235696_at 2.33 down 1.31 down CDNA clone IMAGE:4837650 229483_at 2.09 down 1.31 down CDNA FLJ42331 fis, clone TSTOM2000588 64488_at 2.31 down 1.31 down CDNA FLJ38849 fis, clone MESAN2008936 201674_s_at 2.40 down 1.32 down AKAP1 A kinase (PRKA) anchor protein 1 NM_003488 220342_x_at 2.41 down 1.32 down EDEM3 ER degradation enhancer, mannosidase alpha-like 3 NM_025191 233509_at 2.26 down 1.33 down HERC4 hect domain and RLD 4 NM_015601 /// NM_022079 202386_s_at 2.02 down 1.33 down KIAA0430 KIAA0430 NM_014647 220305_at 2.08 down 1.34 down MGC3260 hypothetical LOC78993 228897_at 2.08 down 1.34 down DERL3 Der1-like domain family, member 3 NM_001002862 /// NM_198440 228723_at 2.14 down 1.34 down CDNA FLJ30445 fis, clone BRACE2009238 239294_at 2.31 down 1.35 down Transcribed locus 208993_s_at 3.01 down 1.35 down PPIG peptidylprolyl isomerase G (cyclophilin G) NM_004792 203264_s_at 2.17 down 1.36 down ARHGEF9 Cdc42 guanine nucleotide exchange factor (GEF) 9 NM_015185 217164_at 2.16 down 1.36 down 218756_s_at 2.12 down 1.36 down MGC4172 short-chain dehydrogenase/reductase NM_024308 228252_at 2.09 down 1.36 down PIF1 PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) NM_025049 235782_at 2.52 down 1.36 down Transcribed locus 211969_at 2.13 down 1.37 down HSP90AA1 heat shock protein 90kDa alpha (cytosolic), class A member 1 NM_001017963 /// NM_005348 215942_s_at 2.01 down 1.37 down GTSE1 G-2 and S-phase expressed 1 NM_016426 227811_at 2.17 down 1.38 down FGD3 FYVE, RhoGEF and PH domain containing 3 NM_001083536 /// NM_033086 210625_s_at 2.35 down 1.38 down AKAP1 A kinase (PRKA) anchor protein 1 NM_003488 210993_s_at 2.24 down 1.38 down SMAD1 SMAD family member 1 NM_001003688 /// NM_005900 224520_s_at 5.02 down 1.39 down BEST3 bestrophin 3 NM_032735 /// NM_152439 227254_at 3.15 down 1.39 down POU2F1 POU class 2 homeobox 1 NM_002697 236090_at 2.16 down 1.39 down Transcribed locus 233642_s_at 2.06 down 1.39 down HEATR5B HEAT repeat containing 5B NM_019024 222305_at 2.08 down 1.39 down HK2 hexokinase 2 NM_000189 214550_s_at 2.60 down 1.40 down TNPO3 transportin 3 NM_012470 222713_s_at 2.03 down 1.40 down FANCF Fanconi anemia, complementation group F NM_022725 242578_x_at 2.83 down 1.40 down SLC22A3 Solute carrier family 22 (extraneuronal monoamine transporter), member 3 NM_021977 222045_s_at 2.23 down 1.41 down PCIF1 PDX1 C-terminal inhibiting factor 1 NM_022104 243560_at 2.54 down 1.42 down Transcribed locus 219949_at 4.30 down 1.42 down LRRC2 leucine rich repeat containing 2 NM_024512 /// NM_024750 203345_s_at 2.28 down 1.42 down MTF2 metal response element binding transcription factor 2 NM_007358 223174_at 2.14 down 1.42 down BTBD10 BTB (POZ) domain containing 10 NM_032320 217807_s_at 2.01 down 1.42 down GLTSCR2 glioma tumor suppressor candidate region gene 2 NM_015710 232682_at 2.06 down 1.42 down MREG melanoregulin NM_018000 242691_at 2.31 down 1.43 down CDNA FLJ41369 fis, clone BRCAN2006117 226413_at 2.05 down 1.43 down LOC400027 hypothetical gene supported by BC047417 XM_001718517 /// XM_931434 /// XM_943193 203725_at 2.08 down 1.43 down GADD45A growth arrest and DNA-damage-inducible, alpha NM_001924 225108_at 2.47 down 1.44 down 229050_s_at 2.11 down 1.44 down SNHG7 small nucleolar RNA host gene (non-protein coding) 7 NR_003672 228167_at 2.01 down 1.44 down KLHL6 kelch-like 6 (Drosophila) NM_130446 1552621_at 2.45 down 1.44 down POLR2J2 DNA directed RNA polymerase II polypeptide J-related NM_032959 239024_at 2.03 down 1.45 down ZNF148 zinc finger protein 148 NM_021964 1558755_x_at 2.66 down 1.45 down ZNF763 zinc finger protein 763 NM_001012753 225330_at 2.20 down 1.45 down IGF1R insulin-like growth factor 1 receptor NM_000875 223487_x_at 2.04 down 1.45 down GNB4 guanine nucleotide binding protein (G protein), beta polypeptide 4 NM_021629 1552427_at 2.35 down 1.46 down ZNF485 zinc finger protein 485 NM_145312 33646_g_at 2.24 down 1.46 down GM2A GM2 ganglioside activator NM_000405 201737_s_at 2.92 down 1.46 down 7-Mar membrane-associated ring finger (C3HC4) 6 NM_005885 212086_x_at 2.03 down 1.47 down LMNA lamin A/C NM_005572 /// NM_170707 /// NM_170708 minichromosome maintenance complex component 3 associated protein 220459_at 2.13 down 1.47 down MCM3APAS antisense NR_002776 230178_s_at 2.02 down 1.47 down CDNA FLJ38461 fis, clone FEBRA2020977 209162_s_at 2.03 down 1.47 down PRPF4 PRP4 pre-mRNA processing factor 4 homolog (yeast) NM_004697 238908_at 2.94 down 1.48 down CALU Calumenin NM_001219 205132_at 2.29 down 1.48 down ACTC1 actin, alpha, cardiac muscle 1 NM_005159 217142_at 2.30 down 1.49 down NM_001042749 /// NM_001042750 /// NM_001042751 /// 207983_s_at 2.21 down 1.49 down STAG2 stromal antigen 2 NM_006603 225640_at 2.15 down 1.49 down LOC401504 hypothetical gene supported by AK091718 241773_at 2.14 down 1.49 down 1553286_at 2.17 down 1.50 down ZNF555 zinc finger protein 555 NM_152791 216606_x_at 2.14 down 1.50 down LYPLA2P1 lysophospholipase II pseudogene 1 NR_001444
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  • Mouse Carboxypeptidase M / CPM Protein (His Tag)
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  • IL21R Expressing CD14+CD16+ Monocytes Expand in Multiple
    Plasma Cell Disorders SUPPLEMENTARY APPENDIX IL21R expressing CD14 +CD16 + monocytes expand in multiple myeloma patients leading to increased osteoclasts Marina Bolzoni, 1 Domenica Ronchetti, 2,3 Paola Storti, 1,4 Gaetano Donofrio, 5 Valentina Marchica, 1,4 Federica Costa, 1 Luca Agnelli, 2,3 Denise Toscani, 1 Rosanna Vescovini, 1 Katia Todoerti, 6 Sabrina Bonomini, 7 Gabriella Sammarelli, 1,7 Andrea Vecchi, 8 Daniela Guasco, 1 Fabrizio Accardi, 1,7 Benedetta Dalla Palma, 1,7 Barbara Gamberi, 9 Carlo Ferrari, 8 Antonino Neri, 2,3 Franco Aversa 1,4,7 and Nicola Giuliani 1,4,7 1Myeloma Unit, Dept. of Medicine and Surgery, University of Parma; 2Dept. of Oncology and Hemato-Oncology, University of Milan; 3Hematology Unit, “Fondazione IRCCS Ca’ Granda”, Ospedale Maggiore Policlinico, Milan; 4CoreLab, University Hospital of Parma; 5Dept. of Medical-Veterinary Science, University of Parma; 6Laboratory of Pre-clinical and Translational Research, IRCCS-CROB, Referral Cancer Center of Basilicata, Rionero in Vulture; 7Hematology and BMT Center, University Hospital of Parma; 8Infectious Disease Unit, University Hospital of Parma and 9“Dip. Oncologico e Tecnologie Avanzate”, IRCCS Arcispedale Santa Maria Nuova, Reggio Emilia, Italy ©2017 Ferrata Storti Foundation. This is an open-access paper. doi:10.3324/haematol. 2016.153841 Received: August 5, 2016. Accepted: December 23, 2016. Pre-published: January 5, 2017. Correspondence: [email protected] SUPPLEMENTAL METHODS Immunophenotype of BM CD14+ in patients with monoclonal gammopathies. Briefly, 100 μl of total BM aspirate was incubated in the dark with anti-human HLA-DR-PE (clone L243; BD), anti-human CD14-PerCP-Cy 5.5, anti-human CD16-PE-Cy7 (clone B73.1; BD) and anti-human CD45-APC-H 7 (clone 2D1; BD) for 20 min.
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