Intercropping Wheat and Barley with Nitrogen Fixing Legume Species in Low Input Organic Systems
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INTERCROPPING WHEAT AND BARLEY WITH NITROGEN FIXING LEGUME SPECIES IN LOW INPUT ORGANIC SYSTEMS by TEJENDRA CHAPAGAIN MSc Agriculture, Tribhuvan University, 2005 A THESIS SUBMITTED IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Plant Science) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) September 2014 © Tejendra Chapagain, 2014 Abstract Declining land productivity associated with decreasing soil organic carbon (SOC) and nitrogen (N) is an issue for conventional production of small grains. Intercropping grains with legumes may provide a sustainable solution. I grew wheat (Triticum aestivum cv. ‘Scarlet’) as a monoculture and intercropped with either common bean (Phaseolus vulgaris cv. ‘Red Kidney’, or cv. ‘Black Turtle’), or fava bean (Vicia faba cv. ‘Bell’) in rows of wheat:bean 1:1 and 2:1 as well as broadcast arrangements to assess the effects of genotype and spatial arrangements on crop agronomy, land productivity, biological nitrogen fixation and transfer, N and carbon (C) accumulation in aboveground biomass, soil N balance, gross ecosystem photosynthesis (GEP), net ecosystem productivity (NEP), and water use efficiency (WUE). Barley (Hordeum vulgare cv. ‘Oxbridge’) and pea (Pisum sativum cv. ‘Reward’) were also included based on synchronized maturity, yield potential, protein content, and root architecture. Stable isotope methods (13C and 15N) coupled with field CO2 exchange measurements were used to determine C and N transformations. Intercrop plots had higher land productivity, improved grain and biomass quality, increased legume nodulation and percent N derived from symbiotic N2 fixation. Wheat-fava bean in the 1:1 arrangement displayed a 50% increase in land productivity. Barley-pea in the 2:1 arrangement also had the highest total land outputs (5.9 t ha-1) and land equivalent ratio (1.32). Wheat-fava bean in the 1:1 arrangement fixed the highest amount of N (74 kg N ha-1), transferred the most N (13% of N in wheat), and accumulated more C (26% higher than wheat monoculture) in shoot biomass. WUE of wheat was improved when grown with fava bean. Pea in intercrop plots also displayed increased nodulation (27-45%) and ii symbiotic N2 fixation (9-17%) leading to the addition of 60-78 kg N ha-1. The GEP and NEP were highest in the 2:1 arrangement and led to the highest daytime C sequestration (229 mg C m-2 hr-1). I demonstrated that intercropping small grains with legumes, in specific spatial arrangements and under low input organic conditions, can counter conventional monoculture-associated SOC and N losses through higher land and ecosystem productivity, and greater organic N-fixation and transfer. iii Preface This thesis entitled ‘Intercropping Wheat and Barley with Nitrogen Fixing Legume Species in Low Input Organic Systems’ is a product of field and lab research carried out at the University of British Columbia - Vancouver. The author is responsible for designing and implementing research activities, data collection, analysis and interpretation of the results, and writing manuscripts presented in this thesis. The findings presented in Chapters 2-5 have been published or accepted as the following publications. Chapagain, T. and A. Riseman (2012). Evaluation of Heirloom and Commercial Cultivars of Small Grains under Low Input Organic Systems. American Journal of Plant Sciences 3 (5): 655-669. Chapagain, T., L. Super and A. Riseman (2014). Root Architecture Variation in Wheat and Barley Cultivars. American Journal of Experimental Agriculture 4 (7): 849-856. Chapagain, T. and A. Riseman (2014). Intercropping Wheat and Beans: Effects on Agronomic Performance and Land Productivity. Crop Science 54 (5): 2285-2293. Chapagain, T. and A. Riseman (2014). Barley-Pea Intercropping: Effects on Land Productivity, Carbon and Nitrogen Transformations. Field Crops Research 166: 18-25. Chapagain, T. and A. Riseman, Nitrogen Transformation, Water Use Efficiency and Ecosystem Productivity in Monoculture and Wheat-Bean Intercropping Systems (Manuscript accepted for publication in Nutrient Cycling in Agroecosystems). iv Table of Contents Abstract....... .............................................................................................................................................................. ii Preface…….. ............................................................................................................................................................. iv Table of Contents .................................................................................................................................................. v List of Tables ........................................................................................................................................................... x List of Figures ...................................................................................................................................................... xiv List of Acronyms and Abbreviations ........................................................................................................... xv Acknowledgements ......................................................................................................................................... xvii Dedication………… ................................................................................................................................................ xx CHAPTER 1: BACKGROUND INFORMATION AND OBJECTIVES ......................................................... 1 1.1 Small Grain Production: Opportunities and Challenges ............................................................. 1 1.1.1 Soil degradation: An emerging issue in small grain production ................................. 3 1.2 Intercropping: An Alternative to Conventional Small Grain Production ............................. 4 1.3 Nitrogen Transfer between Legumes and Associated Non-legume Plants ......................... 6 1.4 Estimating Nitrogen Fixation and Transfer using 15N Isotope Methods.............................. 8 1.4.1 15N natural abundance method ............................................................................................. 10 1.5 Understanding Wheat and Barley Root Architecture .............................................................. 13 1.6 CO2 Uptake, Respiration and Carbon Sequestration ................................................................. 15 1.7 Water Use Efficiency ............................................................................................................................. 17 v 1.8 Research Goal and Specific Objectives ........................................................................................... 18 CHAPTER 2: CULTIVAR EVALUATION TRIAL ........................................................................................ 20 2.1 Materials and Methods ......................................................................................................................... 20 2.1.1 Climate description of the study area ................................................................................. 20 2.1.2 Soil and site description ........................................................................................................... 21 2.1.3 Experimental details .................................................................................................................. 21 2.1.4 Data collection and analysis .................................................................................................... 22 2.2 Results and Discussion ......................................................................................................................... 24 2.2.1 Plant-based parameters ........................................................................................................... 24 2.2.2 Management-based parameters ............................................................................................ 30 2.2.3 Protein content ............................................................................................................................ 33 2.3 Conclusions ............................................................................................................................................... 34 CHAPTER 3: WHEAT AND BARLEY ROOT ARCHITECTURE ............................................................. 57 3.1 Materials and Methods ......................................................................................................................... 57 3.1.1 Cultivar selection ......................................................................................................................... 57 3.1.2 Seed treatment ............................................................................................................................. 57 3.1.3 Study design and set-up ........................................................................................................... 58 3.1.4 Root architecture data analysis ............................................................................................. 58 3.2 Results and Discussion ......................................................................................................................... 59 3.2.1 Wheat root architecture ........................................................................................................... 59 vi 3.2.2 Barley root architecture ..........................................................................................................