DOCSLIB.ORG

The Inheritance of Seed Dormancy in Sinapis Arvensis L

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
The Inheritance of Seed Dormancy in Sinapis Arvensis L Heredity 56 (1986) 25—31 The Genetical Society of Great Britain Received 8 March 1985 The inheritance of seed dormancy in Sinapis arvensis L. K. Garbutt* and J. R. Witcombet School of Plant Biology, University College of North Wales, Bangor, Gwynedd, U.K. Selection for dormant and non-dormant seed in Sinapis arvensis was carried to the seventh and fourteenth generation, respectively. Crosses between the dormant and non-dormant lines clearly showed both a maternal and an embryonic component of seed dormancy. A model for the number of alleles controlling dormancy was constructed and tested. The maternal component of dormancy was shown to be controlled by a single locus with two alleles, the dormant allele being dominant to the non-dormant. No clear picture of the control of the embryonic component of dormancy was found. INTRODUCTION shown a clear response to selection for non- dormancy in several populations. They also Therehave been several reports of genetic control demonstrated a clear difference in germinability of factors affecting seed dormancy in plants. between threshed and unthreshed grains, indicat- Honing (1930) showed a genetic basis and a dis- ing the presence of inhibitors in the lemma and tinct maternal effect in the light dependence of palea, and hence a maternal component to control germination behaviour in tobacco. Harper and of dormancy. McNaughton (1960) found evidence of genetic There have been two recent attempts to estimate control of seed dormancy in species of Papaver. the number of loci involved in the control of germi- New (1958; 1959; 1961) has shown a strict genetic nation. Guenther and Borriss (1975) tried unsuc- control of seed coat types associated with differing cessfully to estimate the number of loci controlling germination rates in Spergula arvensis indicating seed dormancy in Antirrhinum spp. concluding that a strict maternal control, via the seed coat, over dormancy was controlled by a polygenic complex. seed dormancy. However, Jana, Acharya and Naylor (1979) have Naylor and his co-workers have shown genetic shown that at least three loci control after-ripening variability in seed dormancy in Avena fatua in in Avena fatua. response to several environmental variables In the field, Sinapis arvensis populations applied both whilst the seed is maturing and during usually show two "flushes" of germination each germination tests (Naylor and Jana, 1976; Naylor year, one in spring and the other in autumn. and Fedec, 1978; Sawhney and Naylor, 1979). Jam Edwards (1980) suggests that the autumnal popu- (1982) has also demonstrated genetic components lation results from the non-dormant fraction of the of seed dormancy for Arena barbata and Tr(folium seed produced in the spring. In the field, S. arvensis hirtum but in the same study was unable to demon- germination is controlled to some extent by strate a significant genetic component of dormancy environmental factors. Edwards (1980) found in Bromus mollis. germination to be associated with soil temperatures Hilu and de Wet (1980) have investigated the of 44°C at a depth of 10 cm and with high rainfall genetic basis of dormancy in Eleusine sp. and have in spring and simply with adequate moisture in autumn. There is also evidence that at least some Present addresses: *TheBiological Laboratories, Harvard Uni- seed of S. arvensis require light to germinate versity, 16 Divinity Avenue, Cambridge, MA 02138. (Frankland, 1976). In the laboratory, dormancy is t ICRISAT, Pantancheru P.O., Andhra Pradesh 502 324 India. broken by the application of gibberellic acid 26 K. GARBU1T AND J. R. WITCOMBE (Witcombe and Whittington, 1972; Edwards, the parent plants has a seed coat of generation P1 1976). and embryo of generation pe,andthe plants grown The presence of a germination inhibitor and its from this seed will be generation peSeedfrom a mode of action in seeds of S. arvensis was reported cross between two plants of pe generation will in Edwards (1968c). This inhibitor is apparently have Sgenerationseed coat and F generation produced by the seed coat (testa) (Witcombe, embryo. Hiliman and Whittington, 1969) and seems to be All plants were grown in a greenhouse in 10 cm present in the seed coat of all seeds in apparently plastic pots filled with John Innes No. 1 compost. similar quantities (see Edwards, 1968a for a Supplementary light to give a daylength of 16 hours detailed description of seed anatomy). The inhibi- minimum was provided by mercury vapour lamps tion is broken down by oxidation (Edwards, 1969) and temperature was maintained at a minimum of and the effect of the seed coat in dormant seed 18°C. Treatments of fungicide and insecticide were appears to be to prevent this oxidation taking place applied as necessary. by being impermeable to oxygen (Edwards, All pollinations were made in the same manner 1968b). The seed coat is clearly of maternal origin whether within or between lines. Dehiscing anthers and hence control of dormancy is maternal. There were removed from the male parent and placed in is evidence, however, of a further level of dor- a watch glass; a flower bud about to open, but not mancy control, possibly unrelated to this maternal yet showing the stigmatic surface was opened, and effect, and located in the embryo (Edwards, 1968c; emasculated and the newly exposed stigmatic sur- Witcombe, 1971). This points to one of the major face loaded with pollen from the anthers of the problems in studies of the inheritance of seed male parent. The pedicel was marked with paint dormancy. The seed combines parts of three dis- and 24 hours later a further application of pollen tinct generations (and genotypes)—the seed coat was made. (maternal), the endosperm (filial but with one These crosses produced generationseed, paternal and a variable number of maternal 200 individuals of which were grown on and genomes) and the embryo (filial). In the ripe seed allowed to cross at random to produce F7F of Cruciferae, however, the endosperm has disap- generation and seed 200 individuals of which, peared leaving a seed composed of two generations when grown on and crossed, produced FF seed. of tissue, seed coat and embryo. A further set of crosses was made between pe The purpose of the following investigation was plants. These comprised 50 crosses between dor- to determine the manner in which dormancy was mant males and females, 50 crosses between non- controlled genetically in Sinapis arvensis. dormant males and females, and 50 crosses between dormant males and non-dormant females. This was to confirm that the differences observed MATERIALSAND METHODS between the germination rates of seed from non- dormant maternal parents from the p1pe and the Inorder to establish the genetic basis of seed PF generations were, in fact, real and not a dormancy, two selection lines, one for dormancy function of a random difference between genera- (D) and one for non-dormancy (N.D.), were estab- tions. lished from a natural population collected in At each generation, pspePSFC Nottinghamshire by J. R. Witcombe (Witcombe germination tests were carried out on the seed in and Whittington 1972). These lines were taken to closed petri dishes each containing one sheet of the SlO generation for the ND. line and the S5 9.0 cm filter paper. Initially each dish received 5 ml generation for the D line by J. R. Witcombe and of distilled water. Thereafter, all dishes received K. Daniels. The present authors continued distilled water as necessary. The germination tests selection using the same methods as Witcombe were carried out at 25°C, with 24 hour light. The and Whittington (1972) and Witcombe (1971) to lids of the petri dishes were lifted daily to prevent the S14 generation for the N.D. line and S7 the build-up of gases which might inhibit germina- generation for the D line. At this point, 200 tion. Each dish contained 10 seeds unless otherwise reciprocal pairs of crosses were made between the stated. lines and seed produced to the third filial At the conclusion of each test (14 days), the generation. seeds that remained ungerminated were treated As mentioned in the Introduction, the seed is with high levels of G.A. (up to 3000 ig/Iitre) to made up of two distinct generations. Thus a special stimulate dormant seeds into germination and notation will be used. The seed which produces thereby detect non-viable seed. Gibberellic acid INHERITANCE OF SEED DORMANCY 27 was applied in an aqueous solution at pH 7. All generation S7 and then tended to reach a plateau, G.A. solutions were made up freshly before an though with a continued slight increase in germina- experiment and were used immediately. tion and decline in standard error (calculated from With the exception of FF bulk germination arcsine transformation) between S7 and S14 tests were performed (no record of individual plant (fig. 1). performance being kept). The results of the bulk Table 1 gives the mean percentage germination tests were based upon as many seeds as possible and 95 per cent confidence limits for the two lines (between 1000 and 2000 seeds) with only a small at the last generation of selection and the control sample being kept in reserve. Confidence intervals population. The N.D. line is clearly different from were obtained using tables from Rohif and Sokal the others. The difference between the control and (1981), when more than 1000 seeds were used the the D line is small (OO5>p>O0l) from a t-test limits for a sample of 1000 were used as a conserva- on arcsine transformed data. tive estimate. Tests of FF seed were based on Neither 100 per cent germination in the absence individual plants, five petri dishes of 20 seeds being of G.A., nor 0 per cent germination could be set up for each of 100 FF plants.
Load more

Recommended publications
  • Variation of Physical Seed Dormancy and Its Ecological Role in Fire-Prone Ecosystems
    University of Wollongong Research Online University of Wollongong Thesis Collection 1954-2016 University of Wollongong Thesis Collections 2016 Variation of physical seed dormancy and its ecological role in fire-prone ecosystems Ganesha Sanjeewani Liyanage Borala Liyanage University of Wollongong Follow this and additional works at: https://ro.uow.edu.au/theses University of Wollongong Copyright Warning You may print or download ONE copy of this document for the purpose of your own research or study. The University does not authorise you to copy, communicate or otherwise make available electronically to any other person any copyright material contained on this site. You are reminded of the following: This work is copyright. Apart from any use permitted under the Copyright Act 1968, no part of this work may be reproduced by any process, nor may any other exclusive right be exercised, without the permission of the author. Copyright owners are entitled to take legal action against persons who infringe their copyright. A reproduction of material that is protected by copyright may be a copyright infringement. A court may impose penalties and award damages in relation to offences and infringements relating to copyright material. Higher penalties may apply, and higher damages may be awarded, for offences and infringements involving the conversion of material into digital or electronic form. Unless otherwise indicated, the views expressed in this thesis are those of the author and do not necessarily represent the views of the University of Wollongong. Recommended Citation Borala Liyanage, Ganesha Sanjeewani Liyanage, Variation of physical seed dormancy and its ecological role in fire-prone ecosystems, Doctor of Philosophy thesis, School of Biological Sciences, University of Wollongong, 2016.
    [Show full text]
  • Genome-Wide Identification of WRKY Family Genes in Peach and Analysis
    Mol Genet Genomics DOI 10.1007/s00438-016-1171-6 ORIGINAL ARTICLE Genome‑wide identification of WRKY family genes in peach and analysis of WRKY expression during bud dormancy Min Chen1,2,3 · Qiuping Tan1,2,3 · Mingyue Sun1,2,3 · Dongmei Li1,2,3 · Xiling Fu1,2,3 · Xiude Chen1,2,3 · Wei Xiao1,2,3 · Ling Li1,2,3 · Dongsheng Gao1,2,3 Received: 30 September 2015 / Accepted: 18 January 2016 © The Author(s) 2016. This article is published with open access at Springerlink.com Abstract Bud dormancy in deciduous fruit trees is an dormancy. The mean expression levels of six WRKY genes important adaptive mechanism for their survival in cold (Prupe.6G286000, Prupe.1G393000, Prupe.1G114800, climates. The WRKY genes participate in several devel- Prupe.1G071400, Prupe.2G185100, and Prupe.2G307400) opmental and physiological processes, including dor- increased during endodormancy and decreased during eco- mancy. However, the dormancy mechanisms of WRKY dormancy, indicating that these six WRKY genes may play genes have not been studied in detail. We conducted a a role in dormancy in a perennial fruit tree. This informa- genome-wide analysis and identified 58WRKY genes in tion will be useful for selecting fruit trees with desirable peach. These putative genes were located on all eight chro- dormancy characteristics or for manipulating dormancy in mosomes. In bioinformatics analyses, we compared the genetic engineering programs. sequences of WRKY genes from peach, rice, and Arabi- dopsis. In a cluster analysis, the gene sequences formed Keywords WRKY transcription factors · Peach · Bud three groups, of which group II was further divided into dormancy five subgroups.
    [Show full text]
  • Seed Dormancy and the Control of Germination
    Review Blackwell Publishing Ltd Tansley review Seed dormancy and the control of germination Author for correspondence: William E. Finch-Savage1 and Gerhard Leubner-Metzger2 G. Leubner-Metzger 1Warwick HRI, Warwick University, Wellesbourne, Warwick CV35 9EF, UK; 2Institute for Biology II, Tel: +49 761 203 2936 Fax: +49 761 203 2612 Botany/Plant Physiology, Albert-Ludwigs-University Freiburg, D-79104 Freiburg i. Br., Germany Email: [email protected] Received: 23 February 2006 Accepted: 8 April 2006 Contents Summary 501 IV. How is nondeep physiological seed dormancy regulated by the environment? Ecophysiology and modelling 514 I. Introduction 502 V. Conclusions and perspectives 518 II. What is dormancy and how is it related to germination? 502 Acknowledgements 519 III. How is nondeep physiological dormancy regulated within the seed at the molecular level? 509 References 519 Supplementary material 523 Summary Seed dormancy is an innate seed property that defines the environmental con- ditions in which the seed is able to germinate. It is determined by genetics with a substantial environmental influence which is mediated, at least in part, by the plant hormones abscisic acid and gibberellins. Not only is the dormancy status influenced by the seed maturation environment, it is also continuously changing with time following shedding in a manner determined by the ambient environ- ment. As dormancy is present throughout the higher plants in all major climatic regions, adaptation has resulted in divergent responses to the environment. Through this adaptation, germination is timed to avoid unfavourable weather for subsequent plant establishment and reproductive growth. In this review, we present an integrated view of the evolution, molecular genetics, physiology, biochemistry, ecology and modelling of seed dormancy mechanisms and their control of germination.
    [Show full text]
  • Comparative Biology of Seed Dormancy-Break and Germination in Convolvulaceae (Asterids, Solanales)
    University of Kentucky UKnowledge University of Kentucky Doctoral Dissertations Graduate School 2008 COMPARATIVE BIOLOGY OF SEED DORMANCY-BREAK AND GERMINATION IN CONVOLVULACEAE (ASTERIDS, SOLANALES) Kariyawasam Marthinna Gamage Gehan Jayasuriya University of Kentucky, [email protected] Right click to open a feedback form in a new tab to let us know how this document benefits ou.y Recommended Citation Jayasuriya, Kariyawasam Marthinna Gamage Gehan, "COMPARATIVE BIOLOGY OF SEED DORMANCY- BREAK AND GERMINATION IN CONVOLVULACEAE (ASTERIDS, SOLANALES)" (2008). University of Kentucky Doctoral Dissertations. 639. https://uknowledge.uky.edu/gradschool_diss/639 This Dissertation is brought to you for free and open access by the Graduate School at UKnowledge. It has been accepted for inclusion in University of Kentucky Doctoral Dissertations by an authorized administrator of UKnowledge. For more information, please contact [email protected]. ABSTRACT OF DISSERTATION Kariyawasam Marthinna Gamage Gehan Jayasuriya Graduate School University of Kentucky 2008 COMPARATIVE BIOLOGY OF SEED DORMANCY-BREAK AND GERMINATION IN CONVOLVULACEAE (ASTERIDS, SOLANALES) ABSRACT OF DISSERTATION A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the College of Art and Sciences at the University of Kentucky By Kariyawasam Marthinna Gamage Gehan Jayasuriya Lexington, Kentucky Co-Directors: Dr. Jerry M. Baskin, Professor of Biology Dr. Carol C. Baskin, Professor of Biology and of Plant and Soil Sciences Lexington, Kentucky 2008 Copyright © Gehan Jayasuriya 2008 ABSTRACT OF DISSERTATION COMPARATIVE BIOLOGY OF SEED DORMANCY-BREAK AND GERMINATION IN CONVOLVULACEAE (ASTERIDS, SOLANALES) The biology of seed dormancy and germination of 46 species representing 11 of the 12 tribes in Convolvulaceae were compared in laboratory (mostly), field and greenhouse experiments.
    [Show full text]
  • Understanding Molecular Mechanisms of Seed Dormancy for Improved Germination in Traditional Leafy Vegetables: an Overview
    agronomy Review Understanding Molecular Mechanisms of Seed Dormancy for Improved Germination in Traditional Leafy Vegetables: An Overview Fernand S. Sohindji, Dêêdi E. O. Sogbohossou , Herbaud P. F. Zohoungbogbo, Carlos A. Houdegbe and Enoch G. Achigan-Dako * Laboratory of Genetics, Horticulture and Seed Science, Faculty of Agronomic Sciences, University of Abomey-Calavi, 01 BP 526 Tri Postal, Cotonou, Benin; [email protected] (F.S.S.); [email protected] (D.E.O.S.); [email protected] (H.P.F.Z.); [email protected] (C.A.H.) * Correspondence: [email protected]; Tel.: +229-95-39-32-83 Received: 28 October 2019; Accepted: 24 December 2019; Published: 1 January 2020 Abstract: Loss of seed viability, poor and delayed germination, and inaccessibility to high-quality seeds are key bottlenecks limiting all-year-round production of African traditional leafy vegetables (TLVs). Poor quality seeds are the result of several factors including harvest time, storage, and conservation conditions, and seed dormancy. While other factors can be easily controlled, breaking seed dormancy requires thorough knowledge of the seed intrinsic nature and physiology. Here, we synthesized the scattered knowledge on seed dormancy constraints in TLVs, highlighted seed dormancy regulation factors, and developed a conceptual approach for molecular genetic analysis of seed dormancy in TLVs. Several hormones, proteins, changes in chromatin structures, ribosomes, and quantitative trait loci (QTL) are involved in seed dormancy regulation. However, the bulk of knowledge was based on cereals and Arabidopsis and there is little awareness about seed dormancy facts and mechanisms in TLVs. To successfully decipher seed dormancy in TLVs, we used Gynandropsis gynandra to illustrate possible research avenues and highlighted the potential of this species as a model plant for seed dormancy analysis.
    [Show full text]
  • Seed Viability and Dormancy Breaking in Selected Plants in Fabaceae
    Journal of Pharmacognosy and Phytochemistry 2019; 8(5): 616-621 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2019; 8(5): 616-621 Seed viability and dormancy breaking in selected Received: 10-07-2019 Accepted: 12-08-2019 plants in Fabaceae TG Malammanavar Assistant Professor, TG Malammanavar, BK Katenahalli and Manikanta GS Department of Botany, R.T.E.S. College, Ranebennur, Abstract Karnataka, India This study was carried out to investigate to evaluate the seeds germination by conventional method and breaking the seed dormancy and to compare the viability and dormancy between the methods and the BK Katenahalli seeds sample were collected in wild species in Jogimatti forest of different places in the forest. Assistant Professor, Department of Zoology, R.T.E.S. College Ranebennur, Keywords: Seed germination by conventional plant, seed dormancy, seed viability and different methods Karnataka, India breaking of seed dormancy Manikanta GS Introduction Research Scholar, Seed is a key element in plant production that, it exercise a very great influence on the success Kuvempu University and failure of both natural and artificial regeneration (Nwoboshi, 1982) in this case success Shankaraghatta, Karnataka, India depends largely on the quality and quantity of the seeds of desirable species available in the regeneration area. Seeds a dispersal unit of the plant, plays an important role in the higher plant life cycle. Many seeds plants evolve some mechanism to rise successful generation by setting the timing of germination. Therfore, many mature seeds commited to enter a dormant state. Seeds dormancy, the term devoted regarding the inability of viable seed to germinate under the environmental condition favorable for germination.
    [Show full text]
  • Native Seed Propagation Methods
    Native Seed Propagation Methods Seed dormancy. Dormancy is caused by physical and chemical barriers that keep seeds from germinating until conditions are favorable for the seedlings survival (usually spring but fall for some species). A physical or mechanical dormancy is a hard seed coat or waxy layer that keeps water out of the seed. Dormancy caused by physical barriers is overcome in nature in several ways including mechanical rubbing, freezing and thawing, digestion by soil microorganisms, passage through the digestive tracts of animals, and fire. A chemical dormancy occurs when any of a number of chemicals are present in the seed coat that inhibits germination even when the seed is high in moisture content (fully imbibed) and ready to germinate. The chemical or chemicals responsible have to undergo a chemical change to something else or be leached or washed out of the seed before germination will occur. Chemical dormancy in seeds is broken when they are subjected to one or more of the following conditions: 1. Spending a period of time being cold. 2. Spending a period of time being cold and moist. 3. Being in the presence of light for a period of time. 4. Being in the absence of light for a period of time. Most Missouri wildflowers that have chemical dormancy will lose them in nature by spending the winter in the ground cold and moist. Types of Seed Treatments We can fool nature and cause most dormant seeds to germinate. Some of the more common procedures used to overcome seed dormancy are listed and described below.
    [Show full text]
  • Techniques to Improve Seed Germination Allen R
    Techniques to Improve Seed Germination Allen R. Pyle, J. W. Jung Seed Company Understanding Germination Introduction to Seed Germination Having a good understanding of the fundamentals of seed germination helps gardeners improve their success growing seedlings, regardless of how experienced they are. Knowing these fundamentals helps gardeners to understand the “whys” behind the “hows” when germinating seeds and growing seedlings. Remember that seeds are living organisms even before they germinate and begin growing. By paying careful attention to the key factors that regulate germination, gardeners can grow higher quality seedlings with reduced losses from cultural problems and seedling diseases. Seed germination is a fascinating and remarkable process. It involves several different steps. First, seeds absorb water, in a process called “imbibition.” Seeds require moist conditions in order to take up water initially. This is why it is so important to keep the growing medium consistently and evenly moist while seeds are germinating and to ensure there is good contact between the growing medium and the seed. It is also a reason that keeping stored seeds dry, with low moisture content, is important. Interestingly, imbibition is a physical process that will occur whether a seed is alive or dead. After imbibing water, biological processes greatly speed up inside the seed, activating enzymes and mobilizing nutrients to fuel the growth of the initial root, or “radicle.” As water is absorbed, germinating seeds greatly increase their respiration rate and their need for oxygen. The main way that oxygen becomes limiting for seeds is when the media is completely saturated with water, particularly if the seed is covered with media.
    [Show full text]
  • Burrowing Parrots Cyanoliseus Patagonus As Long-Distance Seed Dispersers of Keystone Algarrobos, Genus Prosopis, in the Monte Desert
    diversity Article Burrowing Parrots Cyanoliseus patagonus as Long-Distance Seed Dispersers of Keystone Algarrobos, Genus Prosopis, in the Monte Desert Guillermo Blanco 1,* , Pedro Romero-Vidal 2, Martina Carrete 2 , Daniel Chamorro 3 , Carolina Bravo 4, Fernando Hiraldo 5 and José L. Tella 5 1 Department of Evolutionary Ecology, Museo Nacional de Ciencias Naturales CSIC, 28006 Madrid, Spain 2 Department of Physical, Chemical and Natural Systems, Universidad Pablo de Olavide, Carretera de Utrera, km 1, 41013 Sevilla, Spain; [email protected] (P.R.-V.); [email protected] (M.C.) 3 Departamento de Ciencias Ambientales, Universidad de Castilla-La Mancha, Av. Carlos III s/n, 45071 Toledo, Spain; [email protected] 4 Centre d’Etudes Biologiques de Chizé, UMR 7372, CNRS and La Rochelle Université, F-79360 Beauvoir-sur-Niort, France; [email protected] 5 Department of Conservation Biology, Estación Biológica de Doñana CSIC, 41092 Sevilla, Spain; [email protected] (F.H.); [email protected] (J.L.T.) * Correspondence: [email protected] Abstract: Understanding of ecosystem structure and functioning requires detailed knowledge about plant–animal interactions, especially when keystone species are involved. The recent consideration of parrots as legitimate seed dispersers has widened the range of mechanisms influencing the life cycle of many plant species. We examined the interactions between the burrowing parrot Cyanoliseus Citation: Blanco, G.; Romero-Vidal, patagonus and two dominant algarrobo trees (Prosopis alba and Prosopis nigra) in the Monte Desert, P.; Carrete, M.; Chamorro, D.; Bravo, Argentina. We recorded the abundance and foraging behaviour of parrots; quantified the handling, C.; Hiraldo, F.; Tella, J.L.
    [Show full text]
  • Seed Dormancy in Setaria Lutescens Jorge Nieto-Hatem Iowa State University
    Iowa State University Capstones, Theses and Retrospective Theses and Dissertations Dissertations 1963 Seed dormancy in Setaria lutescens Jorge Nieto-Hatem Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/rtd Part of the Botany Commons Recommended Citation Nieto-Hatem, Jorge, "Seed dormancy in Setaria lutescens " (1963). Retrospective Theses and Dissertations. 2486. https://lib.dr.iastate.edu/rtd/2486 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Retrospective Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. This dissertation has been 63—7263 microfilmed exactly as received NIETO-HATEM, Jorge, 1930- SEED DORMANCY IN SETARIA LUTESCENS. Iowa State University of Science and Technology Ph.D., 1963 Botany University Microfilms, Inc., Ann Arbor, Michigan SEED DOEMANOY IN SETARIA LUTESCENS by Jorge Nieto-Hatem A Dissertation Submitted to the Graduate Faculty in Partial Fulfillment of The Requirements for the Degree of DOCTOR OF PHILOSOPHY Major Subject: Plant Physiology Approved: Signature was redacted for privacy. In Charge of Major Signature was redacted for privacy. 'ead of Major epartment Signature was redacted for privacy. D duate uollege Iowa State University Of Science and Technology Ames, Iowa 1963 il TABLE OF CONTENTS Page INTRODUCTION 1 LITERATURE REVIEW 2 Observed Dormancy Patterns 2 Dormancy as Determined by Seed Structure 4 Metabolic Aspects of Seed Dormancy and Germination 6 MATERIALS AND METHODS 13 Germination Tests 13 Embryo Culture Techniques 14 Biochemical Analyses 16 Sugar determinations .
    [Show full text]
  • Tallgrass Prairie Center's Native Seed Production Manual
    Native Plant Propagation Websites have also relied extensively on methods described by Norman Deno (Seed Germination Theory and Practice), Dr. Pavel Slaby Native Plant Information Network, Lady Bird Johnson (http://web.kadel.cz/flora) and Tom Clothier (www.anet-chi. Wildflower Center com/~manytimes/index.htm). http://wildflower.utexas.edu/ Manual of Grasses for North America, Utah State University. The PLANTS Database, USDA, NRCS. 2006 <http://herbarium.usu.edu/grassmanual/ > Species distribution http://plants.usda.gov. National Plant Data Center, Baton maps, good line illustrations, taxanomic treatments, etc. Rouge, LA 70874-4490 USA. The PLANTS Database provides standardized information about vascular plants, mosses, Grasses of Iowa. Iowa State University Department of Ecology, liverworts, hornworts, and lichens of the U.S. and its territories. Evolution, and Organismal Biology It includes names, plant symbols, checklists, distributional data, http://www.eeob.iastate.edu/research/iowagrasses/index.html. species abstracts, characteristics, images, crop information, automated tools, onward Web links, and references. This Green Landscaping with Native Plants Environmental information primarily promotes land conservation in the United Protection Agency (EPA) States and its territories, but academic, educational, and general http://www.epa.gov/greenacres/ website for native landscaping use is encouraged. PLANTS reduces government spending in Mid-West by minimizing duplication and making information exchange possible across agencies and disciplines. Native Plant Propagation Protocol Database, sponsored by Native Plant Propagation/ID Books the NativePlants Journal http://www.nativeplantnetwork.org/network/. The Native Plant An illustrated guide to Iowa prairie plants. Christiansen P, Network is devoted to the sharing of information on how to and M.
    [Show full text]
  • Plant Ecology
    Copyedited by: SU Journal of Plant Ecology Physiological dormancy broken by endozoochory: Austral parakeets (Enicognathus ferrugineus) as legitimate dispersers of Downloaded from https://academic.oup.com/jpe/article/13/5/538/5872557 by CSIC user on 30 September 2020 calafate (Berberis microphylla) in the Patagonian Andes Carolina Bravo1,2,*, , Daniel Chamorro3, Fernando Hiraldo4, Karina Speziale5, Sergio A. Lambertucci5, José L. Tella4 and Guillermo Blanco1 1Department of Evolutionary Ecology, Museo Nacional de Ciencias Naturales, CSIC, José Gutiérrez Abascal 2, 28006 Madrid, Spain, 2Centre d’Etudes Biologiques de Chizé, UMR 7372, CNRS and La Rochelle Université, F-79360 Beauvoir-sur- Niort, France, 3Departamento de Ciencias Ambientales, Universidad de Castilla-La Mancha, Av. Carlos III s/n, 45071 Toledo, Spain, 4Department of Conservation Biology, Estación Biológica de Doñana, CSIC, Américo Vespucio 26, 41092 Sevilla, Spain, 5Grupo de Investigaciones en Biología de la Conservación, Departamento de Ecología, INIBIOMA (CONICET-National University of Comahue), Pasaje Gutiérrez 1125, 8400 Bariloche, Argentina *Corresponding author. E-mail: [email protected] Handling Editor: Jana Petermann Received: 13 January 2020, Revised: 15 June 2020, Accepted: 10 July 2020, Advanced Access publication: 16 July 2020 Citation: Bravo C, Chamorro D, Hiraldo F, et al. (2020) Physiological dormancy broken by endozoochory: Austral parakeets (Enicognathus ferrugineus) as legitimate dispersers of calafate (Berberis microphylla) in the Patagonian Andes. J Plant Ecol 13:538–544. https://doi.org/10.1093/jpe/rtaa041 Abstract Aims Seed dispersal by endozoochory is an important process in plant regeneration and the establishment of new populations. Seeds with dormancy may especially benefit after disperser gut passage. However, the ways in which gut passage affect the germination of plant species with physiological dormancy remain unclear.
    [Show full text]