Health Sciences Program Facilities and Equipment Guide April 1, 2021
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Laboratory Exercises in Microbiology: Discovering the Unseen World Through Hands-On Investigation
City University of New York (CUNY) CUNY Academic Works Open Educational Resources Queensborough Community College 2016 Laboratory Exercises in Microbiology: Discovering the Unseen World Through Hands-On Investigation Joan Petersen CUNY Queensborough Community College Susan McLaughlin CUNY Queensborough Community College How does access to this work benefit ou?y Let us know! More information about this work at: https://academicworks.cuny.edu/qb_oers/16 Discover additional works at: https://academicworks.cuny.edu This work is made publicly available by the City University of New York (CUNY). Contact: [email protected] Laboratory Exercises in Microbiology: Discovering the Unseen World through Hands-On Investigation By Dr. Susan McLaughlin & Dr. Joan Petersen Queensborough Community College Laboratory Exercises in Microbiology: Discovering the Unseen World through Hands-On Investigation Table of Contents Preface………………………………………………………………………………………i Acknowledgments…………………………………………………………………………..ii Microbiology Lab Safety Instructions…………………………………………………...... iii Lab 1. Introduction to Microscopy and Diversity of Cell Types……………………......... 1 Lab 2. Introduction to Aseptic Techniques and Growth Media………………………...... 19 Lab 3. Preparation of Bacterial Smears and Introduction to Staining…………………...... 37 Lab 4. Acid fast and Endospore Staining……………………………………………......... 49 Lab 5. Metabolic Activities of Bacteria…………………………………………….…....... 59 Lab 6. Dichotomous Keys……………………………………………………………......... 77 Lab 7. The Effect of Physical Factors on Microbial Growth……………………………... 85 Lab 8. Chemical Control of Microbial Growth—Disinfectants and Antibiotics…………. 99 Lab 9. The Microbiology of Milk and Food………………………………………………. 111 Lab 10. The Eukaryotes………………………………………………………………........ 123 Lab 11. Clinical Microbiology I; Anaerobic pathogens; Vectors of Infectious Disease….. 141 Lab 12. Clinical Microbiology II—Immunology and the Biolog System………………… 153 Lab 13. Putting it all Together: Case Studies in Microbiology…………………………… 163 Appendix I. -
Lab 2 Practical Microbiology
Lab 2 Practical Microbiology Sterilization Sterilization :- is the killing or removal of all microorganisms, including bacterial spores which are highly resistant. Sterilization is an absolute term, meaning the absence of all microorganisms. Disinfection:- is the killing of many, but not all microorganisms. It is a process of reduction of number of contaminating organisms to a level that cannot cause infection, some organisms and bacterial spores may survive. Disinfectants:- are chemicals that are used for disinfection. Disinfectants should be used only on inanimate objects (non-living objects). Antiseptics :- are mild forms of disinfectants that are used externally on living tissues to kill microorganisms, on the surface of skin and mucous membranes. 1 Lab 2 Practical Microbiology CLASSIFICATION OF METHODS Sterilization and disinfection are done by : (A). Physical methods (B). Chemical methods (A). Physical methods. 1. Heat 2. Radiation 3. Filtration 1. Heat:- A. Sterilization by Dry Heat Mechanisms. (1) Protein denaturation, (2) Oxidative damage, (3) Toxic effect of elevated electrolyte (in absence of water). 1- Flaming. The article is passed through flame without allowing it to become red hot, e.g. scalpel. Temperature is not high to cause sterilization. 2- Incineration. Wire loops used in microbiology laboratory are sterilized by heating to 'red' in bunsen burner. 3- Hot Air Oven . It Is one of the most common method used for sterilization. Glass wares, swab sticks, all-glass syringes, powder and oily substances are sterilized in hot air oven. For sterilization, a temperature of 160°C is maintained (holding) for one hour. Spores are killed at this temperature. It leads to sterilization B. -
Study of Equipment's Used in Microbiology : Spirit Lamp, Inoculation Loop, Hot Air Oven, Laminar Air Flow (Laf) and Incubato
Study of some equipments Spirit lamp, Inoculation Loop, Hot Air Oven, Laminar Air Flow (LAF) and Incubator By Dr.S.V.Patil, Head, Department of Botany Bhusawal Arts, Science and P.O. Nahata Commerce college,1 Bhusawal Spirit Lamp Alcohol lamp Used for heating , sterilization and combustion. Ethyl alcohol or spirit used as a fuel. Used to proudest open fire. Made of glass, brass, aluminum. Chemical or biological reaction need to heat to get desired product. Flame is limitated i.e. ( 5 centimeters) in height. Lower temp. than glass flame. Flame sterilization of laboratory equipments. 2 Inoculation Loop Aseptic transfer. Loop consists of insulation Handle. screw device at the top. Heat resistance nichrome or platinum wire. Approximately 3 inch long. Wire end is bent round to form a loop. It sterilized by using heating or flaming until it is red hot. Loop mainly used transfer( sub culture) form liquid culture. 3 Hot Air Oven Used for Sterilizing. glassware, petri dishes, test tube, pipettes, metal instrument. Consist of isolated cabinet which held at a constant temp. Sterilization completed 150 – 180 c for 2-4 hrs. Fan fitted in hot air to circulating at a constant temp. Normal sterilization at 160 0C 4 Laminar Air Flow Its allowed to working for long period. Made up of stainless steel with no gaps or joint. PRINCIPLE – based on flow of current to create uniform velocity along parallel line, which helps in transforming microbial culture in aseptic condition to avoid the dust and contamination. Working - filter pad a fan and HEPA filter ( High Efficiency Particulate Air) 5 Fan suck the air through the filter pad where dust is trapped Prefiltered air has to pass the HEPA filter where contamination fungi, bacteria, dust are removed Ultraclean air which is free from fungal & bacterial contamination flows at the velocity of about 27 ± 3 m/minute through work area UV lamp is fitted in working area 6 Before starting work, LAF is put on for 10 – 15 minute. -
Medical Laboratory Science Program BIOSAFETY/SAFETY MANUAL ______
Medical Laboratory Science Program BIOSAFETY/SAFETY MANUAL ____________________________________________________________________________ ONTARIO TECH UNIVERSITY Medical Laboratory Science Program BIOSAFETY/SAFETY MANUAL Revised: 2019 Page 1 of 69 Medical Laboratory Science Program BIOSAFETY/SAFETY MANUAL ____________________________________________________________________________ Table of Contents 1.0 Introduction 1.1 Definition of Biohazard 1.2 Routine Practices 1.3 Containment 1.3.1 Containment Levels 2.0 MLSc Safety Committee 2.1 Terms of Reference 2.2 Membership 3.0 General Operational Practices 4.0 Laboratory Hygiene 4.1 Immunization 4.2 Dress Code 4.3 Hand Hygiene 4.3.1 Hand Washing 4.3.2 Alcohol Based Antiseptic Hand Cleaners 5.0 Personal Protective Equipment 5.1 Coats 5.1.1 Lab Coats 5.1.2 Rubber Apron 5.2 Gloves 5.2.1 Gloves 5.2.1.1 Proper Removal of Gloves 5.2.2 Chemical Resistant Gloves 5.2.3 Insulated Gloves 5.3 Eye and Face Protection 5.3.1 Goggles and Safety Glasses 5.3.2 Face Shields 5.3.3 Masks and Respirators 5.3.3.1 Procedure for Putting on a Mask 5.4 Donning and Doffing 5.4.1 Procedure for Donning 5.4.2 Procedure for Doffing 6.0 Protective Equipment 6.1 Eye Wash Stations 6.1.1 Procedure for the use of eye wash station 6.2 Safety Shower 6.2.1 Procedure for the use of safety shower 6.3 Fume Hood Page 2 of 69 Medical Laboratory Science Program BIOSAFETY/SAFETY MANUAL ____________________________________________________________________________ 6.3.1 Procedure for using a large floor model fume hood 6.4 Biosafety Cabinet -
Hot-Air-Oven.Pdf
RASAYAN SINCE1969 HOLY SCIENTIFIC THE ULTIMATE SOLUTION OF LAB INSTRUMENTS Hot air oven INTRODUCTION: HOLYSCIENTIFIC is one of the leading science equipment manufacturer & exporter of science equipments, science instruments, lab equipments, lab instruments, testing equipments, testing instruments, like Digital bio-safety cabinet, vertical & horizontal laminar air flow cooling & heating instruments like ultra low temperature deep freezers, BOD incubators, hot air oven, muffle furnace, microscope, fume hood environmental test chamber, water distillation plant, SDI kit, ocean grapy meter, water testing kit, PH meter, soil samplers & all types of laboratory & science Delete text and place photo here. instruments. BETTER TECHNOLOGY & BEST QUOLITY This laboratory & scientific instruments used for WITH FAST SERVICE laboratory sterilization, clean room, microbiology, pathology, bio technology, pharmaceuticals, seed & soil testing, metallurgical, food processing, customized instruments LAB INSTRUMENTS SCIENTIFIC INSTRUMENTS LAB EQUIPMENTS SCIENTIFIC EQUIPMENTS TESTING KITS SCIENTIFIC KITS LABORATORY GLASS WARES LABORATORY PLASTICWARES HOLY SCIENTIFIC LABORATORY CHEMICALS C-2 (6) plot no.: 67 /3 GIDC estate, phase – 1 Vatva, Ahmedabad Gujarat-382445 Contact us: +91(079) 40085092/ 93/ 94 mobile: - +91-09427000891 Fax: 25834006 www.holyscientific.com RASAYAN Hot air oven Hot air oven SCOPE:- The RASAYAN HOT AIR OVEN has been designed to dry samples/glass wares or to perform dry heat sterilization in a laboratory. Lab Hot Air Oven are suitable for various applications in the fields of agricultural, medical and industrial researches for heating, drying, sterilizing and baking in laboratories, hospitals and industries. CONSTRUCTION:- The inner chamber of the Hot Air Oven is made of stainless steel and outside body is made of MS, duly painted/powder coated. -
Egyptians Used Fire for Sterilisation • Greeks Burned Sulphur for Fumigation • Advanced Techniques
• Egyptians used fire for sterilisation • Greeks burned sulphur for fumigation • Advanced techniques To Prevent • Transmission of diseases. • Spoilage of food. • Contamination of pure cultures in laboratories. • Interference of unwanted microbes in industrial process. • Research studies. Choice of Anti microbial agents depends on • Type of microbe • Stages of growth and number • Surroundings 5 METHODS/AGENTS OF STERILIZATION Classification of Sterilization: Sterilization Physical methods. Chemical methods. 6 Physical agents: Sunlight Drying Heat Dry heat: flaming, incineration, hot air Moist heat: pasteurization, boiling, steam under pressure. Filtration: candles, asbestos pads, membranes Radiation Ultrasonic vibrations. Sun light: • Sun light: – Active germicidal effect due to its content of ultraviolet rays . – Natural method of sterilisation of water in tanks, rivers and lakes. 12/2/2012 Dr.T.V.Rao MD 7 Drying: Moisture is essential for growth of bacteria. Drying in air has deleterious effect on many bacteria. However, spores are unaffected. Heat: • Most reliable method of sterilization and should be the method of choice. Dry Heat & Moist Heat. The factors influencing sterilization by heat: • Nature of heat-dry or moist • Temperature and time • Number of microorganisms present • Characteristics of organisms –species, strain, sporing capacity • Type of material from which organism have to be eliminated. • Killing effect is due to protein denaturation, oxidative damage and toxic effect of elevated level of electrolytes. • Killing effect of moist heat due to denaturation and coagulation of proteins. Thermal Death Time:TDT “Minimum time required to kill a suspension of organisms at a predetermined temperature in a specified environment. Thermal death time is inversely proportional to temperature. TDT is increased in presence of organic substance, proteins, nucleic acid, starch, gelatin , sugar , fats, oils.” Heat : • Dry heat: • 1.Red heat 2.Flaming 3.Incineration 4.Hot air oven 12/2/2012 Dr.T.V.Rao MD 11 Dry heat: 1. -
Medical Laboratory Science Program BIOSAFETY/SAFETY MANUAL ______
Medical Laboratory Science Program BIOSAFETY/SAFETY MANUAL ____________________________________________________________________________ UNIVERSITY OF ONTARIO INSTITUTE OF TECHNOLOGY Medical Laboratory Science Program BIOSAFETY/SAFETY MANUAL Revised: 2017 Page 1 of 52 Medical Laboratory Science Program BIOSAFETY/SAFETY MANUAL ____________________________________________________________________________ Table of Contents 1. Introduction 1.1. Definition of Biohazard 1.2. Routine Practices 1.3. Containment 1.3.1 Containment Levels 2. MLSc Safety Committee 2.1. Terms of Reference 2.2. Membership 3. General Operational Practices 4. Laboratory Hygiene 4.1. Immunization 4.2. Dress Code 4.3. Hand Hygiene 4.3.1. Hand Washing 4.3.2. Alcohol Based Antiseptic Hand Cleaners 5. Personal Protective Equipment 5.1. Coats 5.1.1. Lab Coats 5.1.2. Gowns 5.1.3. Rubber Apron 5.2. Gloves 5.2.1. Examination Gloves 5.2.1.1. Proper Removal of Examination Gloves 5.2.2. Chemical Resistant Gloves 5.2.3. Insulated Gloves 5.3 Eye and Face Protection 5.3.1. Goggles and Safety Glasses 5.3.2. Face Shields 5.3.3. Masks and Respirators 5.3.3.1. Procedure for Putting on a Mask 5.4. Donning and Doffing 5.4.1 Procedure for Donning 5.4.2 Procedure for Doffing 6. Protective Equipment 6.1. Eye Wash Stations 6.1.1 Procedure for the use of eye wash station 6.2. Safety Shower 6.2.1 Procedure for the use of safety shower Page 2 of 52 Medical Laboratory Science Program BIOSAFETY/SAFETY MANUAL ____________________________________________________________________________ 6.3. Fume Hood 6.3.1 Procedure for using a large floor model fume hood 6.4. -
OHAUS Balances Navigator Range
Catalogue OHAUS Balances Navigator Range Has Landed Page 120 Parafilm M New Frontier Up to 60% Off Page 4 Centrifuges German Made Page 148 Bollé Safety Schott-DURAN Rush Plus Small Has Arrived Youtility B ottles Page 77 A New Age SAVINGS Up to 70% off major brands Bacto Laboratories Nov 2020 BactoIssue Laboratories 15.0 Pty Ltd - Phone (02) 9823-9000Proudly - Email [email protected] Supplying - (prices Science excl GST) Since 1966 1 Contents A Centrifuge 145 Flask, Volumetric Glass 17 Alcohol Wipes 55 Centrifuge Tube, Glass 24 Flask, Volumetric Plastic 29 Analytical Balance 117 Centrifuge Tube, Plastic 44 Forceps 88 Aspirator Bottle, Plastic 42 Chemicals 67 Forceps, Artery 92 Autoclave Indicators 59 Clamps, Bosshead 96 Fridge Thermometers 102 Autoclave Tape 59 Clamps, Burettes 97 Funnel, Glass 15 Autoclave Waste Bags 61 Clamps, Retort 96 Funnel, Plastic 30 Clinical Centrifuge 145 G - H B Coats, Laboratory 74 Gloves, Examination 72 Bacticinerator 82 Cold Bricks 69 Gloves, Safety 71 Bags, Waste 61 Colony Counter 81 Gowns 75 Balance, Moisture 119 Conductivity Meter 109 Hand Wash, Alcohol 55 Balances 116 Conical Measure, Plastic 27 Hand Wash, Cleanser 56 Batteries 102 Contaminated Waste Bags 62 Hand Wash, Sanitiser 55 Beaker, Glass 11 Coplin Jar 53 Hockey Stick 50 Beaker, Plastic 27 Corrosive Cabinets 85 Hotplate 124 Bench Roll 58 Cover Slips, Glass 52 Hotplate Stirrer 124 Biju McCartney Bottle 25 Cryo Vial, Plastic 37 Hygrometers 102 Bins, Broken Glass 61 Culture Media 50 I Bins, Chemotherapy 65 Culture Tube, Glass 24 Incubator 138 Bins, -
Laboratory Supplies and Equipment
Laboratory Supplies and Equipment Beakers: 9 - 12 • Beakers with Handles • Printed Square Ratio Beakers • Griffin Style Molded Beakers • Tapered PP, PMP & PTFE Beakers • Heatable PTFE Beakers Bottles: 17 - 32 • Plastic Laboratory Bottles • Rectangular & Square Bottles Heatable PTFE Beakers Page 12 • Tamper Evident Plastic Bottles • Concertina Collapsible Bottle • Plastic Dispensing Bottles NEW Straight-Side Containers • Plastic Wash Bottles PETE with White PP Closures • PTFE Bottle Pourers Page 39 Containers: 38 - 42 • Screw Cap Plastic Jars & Containers • Snap Cap Plastic Jars & Containers • Hinged Lid Plastic Containers • Dispensing Plastic Containers • Graduated Plastic Containers • Disposable Plastic Containers Cylinders: 45 - 48 • Clear Plastic Cylinder, PMP • Translucent Plastic Cylinder, PP • Short Form Plastic Cylinder, PP • Four Liter Plastic Cylinder, PP NEW Polycarbonate Graduated Bottles with PP Closures Page 21 • Certified Plastic Cylinder, PMP • Hydrometer Jar, PP • Conical Shape Plastic Cylinder, PP Disposal Boxes: 54 - 55 • Bio-bin Waste Disposal Containers • Glass Disposal Boxes • Burn-upTM Bins • Plastic Recycling Boxes • Non-Hazardous Disposal Boxes Printed Cylinders Page 47 Drying Racks: 55 - 56 • Kartell Plastic Drying Rack, High Impact PS • Dynalon Mega-Peg Plastic Drying Rack • Azlon Epoxy Coated Drying Rack • Plastic Draining Baskets • Custom Size Drying Racks Available Burn-upTM Bins Page 54 Dynalon® Labware Table of Contents and Introduction ® Dynalon Labware, a leading wholesaler of plastic lab supplies throughout -
Chemistry Lab Equipment ID Wksht
Chemistry Lab Equipment ID Activity CLASS SET- Do NOT take! Directions: A number of items you will be using in the laboratory are shown below. Use the word bank to correctly identify the lab equipment. Part One: 14. 1. 2. 13. 12. 3. 11. 100ml \ J' 10. ! i 4. 5. 8. t t 6. 7. 9. WORD BANK: graduated cylinder, lab stand, eye dropper/pipette, beaker, triple beam balance, Bunsen burner, goggles, tongs, funnel, striker, ring stand, Erlenmeyer flask, lab coat, test tube CLASS SET- Do NOT take! Available online if you need to finish Chemistry Lab Equipment ID Activity CLASS SET- Do NOT take! Part Two: Directions: Match the correct equipment pictured on the other side to the tasks asked for below. Obj,ect Nlam,e Used For Us,ed to pi,ck up or ho Id hot olbj,ects 1. Pmtects th,e ey,es from flying objects or chemicalll spllashes 2. A wiide-m,outhed container used to transport, heat or store 3. substances A small gll'ass ,container us,ed to view ,chemical! readmns 4. or to heat small amounts ,of a substance A device to m,easure the mass or "mass ,out" and obj:ed or 5. substance. Pmtects the scientist and the sd enti1st's clotlhes fiiom 6. haza~dous or hot ,chemicals Us,ed to dispense a v,ery small amount of a lli:quiid 7. Us,ed to rght a Bunsen burner 8. Attaches to a lalb stand and used to hold a va1riety of llab 9. equiip,ment Us,ed to m,easure vollume v,e1ry prec.lisely 10. -
Toxcity Tests for Freshwater Organisms
DFO - Libra y MPO Biblio hèque III II I 11 1111 1204304311 1111 II Canadian Special Publication of Fisheries and Aquatic Sciences 44 einellme ---.211151m.yeee - 7,11r,r1 ft Perielin e—N Edited by E. Scherer QL 626 C314 no.44 c.2 1979 Government of Canada Gouvernement du Canada Fisheries and Oceans Pêches et Océans CANADIAN SPECIAL PUBLICATION OF FISHERIES AND AQUATIC SCIENCES 44 TOXICITY TESTS FOR FRESHWATER ORGANISMS Edited by E. SCHERER Department of Fisheries and Oceans Freshwater Institute Winnipeg, Manitoba R3T 2N6 DEPARTMENT OF FISHERIES AND OCEANS WINNIPEG, MANITOBA 1979 In addition to the Canadian Special Publications of Fisheries and Aquatic Sciences (formerly Miscellaneous Special Publications), the Department of Fisheries and Oceans publishes the Canadian Journal of Fisheries and Aquatic Sciences (formerly the Journal of the Fisheries Research Board of Canada) in annual volumes of monthly issues,and a Bulletin series. These publications are for sale from the Canadian Government Publishing Centre, Supply and Services Canada, Hull, Quebec, Canada KlA 059. Remittance must be made in advance, payable in Canadian funds, to the order of the Receiver General for Canada. THIS PUBLICATION IS AVAILABLE FREE FROM THE FRESHWATER INSTITUTE ED Minister of Supply and Services Canada 1979 Cat. No. Es 41-31/44 ISSN 0706-6481 Correct citation for this publication: Scherer, E. (ed.) 1979. Toxicity tests for freshwater organisms. Can. Spec. Publ. Fish. Aquat. Sci. 44: 194 p. Cover design by Don i A. Taite CONTENTS Page Preface Acknowledgements ix Abstract Résumé DE MARCH, B.G.E.: Reproduction and growth impairment tests with the amphipod Hyalella azteca . -
Holy Scientific the Ultimate Solution of Lab Instruments
RASAYAN SINCE1969 HOLY SCIENTIFIC THE ULTIMATE SOLUTION OF LAB INSTRUMENTS Bomb calorimeter INTRODUCTION: HOLYSCIENTIFIC is one of the leading science equipment manufacturer & exporter of science equipments, science instruments, lab equipments, lab instruments, testing equipments, testing instruments, like Digital bio-safety cabinet, vertical & horizontal laminar air flow cooling & heating instruments like ultra low temperature deep freezers, BOD incubators, hot air oven, muffle furnace, microscope, fume hood environmental test chamber, water distillation plant, SDI kit, ocean grapy meter, water testing kit, PH meter, soil samplers & all types of laboratory & science instruments. BETTER TECHNOLOGY & BEST QUOLITY This laboratory & scientific instruments used for WITH FAST SERVICE laboratory sterilization, clean room, microbiology, LAB INSTRUMENTS pathology, bio technology, pharmaceuticals, seed & soil testing, metallurgical, food processing, customized SCIENTIFIC INSTRUMENTS instruments LAB EQUIPMENTS LAB INSTRUMENTS TESTING KITS SCIENTIFIC KITS LABORATORY GLASS WARES LABORATORY PLASTICWARES LABORATORY CHAMICALS HOLY SCIENTIFIC C-2 (6) plot no.: 67 /3 GIDC estate, phase – 1 Vatva, Ahmedabad Gujarat-382445 Contact us: +91(079) 40085092/ 93/ 94 mobile: - +91-09427000891 Fax: 25834006 www.holyscientific.com RASAYAN Bomb calorimter Bomb calorimeter SCOPE:- We are the leading manufacturer of BOMB CALORIMETER in region. Our bomb calorimeters are widely used for day to day determination of Calorific value Liquids & Solids. We specialize in both