Cell Biology Annual Meeting Program
Shirley Tilghman, President Julie Theriot, Program Chair Karen Oegema, Local Organizer
ascb.org/2015meeting
Search Your Smartphone App Store for ASCB2015 Submit Your Research
Committed to Excellence, Rigor, and Breadth eNeuro has joined JNeurosci in the PubMed Central database.
Learn more at SfN.org DON’T MISS OUT ON ANOTHER ISSUE! SUBSCRIBE Sign up now to receive FOR FREE* the monthly print magazine. TODAY! Each issue contains feature articles on hot new trends in science, profiles of top-notch researchers, reviews of the latest tools and technologies, and much, much more.
* Only available for a limited time. Not all requests qualify for a free subscription. www.the-scientist.com/subscribe
The Company of Biologists is a UK based charity and not-for-profit publisher run by biologists for biologists. The Company aims to promote research and study across all branches of biology through the publication of its five journals.
Development Advances in developmental biology and stem cells dev.biologists.org Journal of Cell Science The science of cells jcs.biologists.org The Journal of Experimental Biology At the forefront of comparative physiology and integrative biology jeb.biologists.org
Disease Models & Mechanisms Basic research with translational impact dmm.biologists.org Biology Open Facilitating rapid peer review for accessible research bio.biologists.org
In addition to publishing, The Company makes an important contribution to the scientific community, providing grants, travelling fellowships and sponsorship to noteworthy scientists, meetings, societies and collaborative projects around the world. The Company also runs a series of transdisciplinary workshops. For subscriptions and consortia sales email [email protected] Recommend a subscription by completing our library recommendation form http://biologists.com/downloads/Library.pdf For more information please visit our website biologists.com THERE’S A SCIENCE TO ADVANCING YOUR CAREER
The Company of Biologists is a UK based charity and not-for-profit publisher run by biologists for biologists. The Company aims to promote research and study across all branches of biology through the publication of its five journals.
Development Advances in developmental biology and stem cells dev.biologists.org For your career in science, there’s only one Journal of Cell Science The science of cells jcs.biologists.org Access jobs and career resources—all for free The Journal of Experimental Biology At the forefront of comparative physiology and integrative biology § Search thousands of job listings § Read relevant career advice articles jeb.biologists.org § Complete an interactive, personalized § Research graduate programs career plan at “my IDP” Disease Models & Mechanisms § Visit our Career Forum to get advice. Basic research with translational impact § Create job alerts based on your criteria dmm.biologists.org § View webinars and booklets Biology Open Stop by Booth Facilitating rapid peer review for accessible research § Post your resume/CV in our # for a gift bio.biologists.org searchable database
In addition to publishing, The Company makes an important contribution to the scientific community, providing grants, travelling fellowships and sponsorship to noteworthy scientists, meetings, societies and Advance your career. Visit collaborative projects around the world. The Company also runs a series of transdisciplinary workshops. ScienceCareers.org/ASCB For subscriptions and consortia sales email [email protected] Recommend a subscription by completing our library recommendation form http://biologists.com/downloads/Library.pdf For more information please visit our website biologists.com ANNUAL REVIEWS SPARK A CONNECTION
Annual Review of Cell and Developmental Biology cellbio.annualreviews.org • Volume 31 • November 2015 Editor: Randy Schekman, University of California, Berkeley The Annual Review of Cell and Developmental Biology, in publication since 1985, covers the most significant developments in the fields of cell and developmental biology, including structure, function, and organization of the cell; development and evolution of the cell as it relates to single and multicellular organisms; and the models and tools of molecular biology and imaging.
Discounted personal copy pricing is available for ASCB Members. Contact the ASCB for details.
TABLE OF CONTENTS: • Toward a Synthesis of Developmental Biology with Evolutionary • Perspective, Lewis Wolpert Theory and Ecology, Ralf J. Sommer, Melanie G. Mayer • Sizing up to Divide: Mitotic Cell-Size Control in Fission Yeast, • Metabolism and Epigenetics, Ryan Janke, Anne E. Dodson, • Elizabeth Wood, Paul Nurse Jasper Rine • Translating the Genome in Time and Space: Specialized Ribosomes, • Stress Signaling Between Organs in Metazoa, Edward Owusu-Ansah, RNA Regulons, and RNA-Binding Proteins, Zhen Shi, Maria Barna Norbert Perrimon • Motors, Anchors, and Connectors: Orchestrators of Organelle • Placenta: The Forgotten Organ, Emin Maltepe, Susan J. Fisher Inheritance, Barbara Knoblach, Richard A. Rachubinski • Lung Endoderm Morphogenesis: Gasping for Form and Function, • Mechanism and Regulation of Cytoplasmic Dynein, Daniel T. Swarr, Edward E. Morrisey Michael A. Cianfrocco, Morgan E. DeSantis, Andres E. Leschziner, • Polarized Protein Transport and Lumen Formation During Epithelial Samara L. Reck-Peterson Tissue Morphogenesis, Alex J. Blasky, Anthony Mangan, Rytis Prekeris • The Pathway of Collagen Secretion, Vivek Malhotra, Patrik Erlmann • Structure, Regulation, and Functional Diversity of Microvilli on the • The Hepatitis B Virus Receptor, Wenhui Li Apical Domain of Epithelial Cells, Cécile Sauvanet, Jessica Wayt, • Prions: What Are They Good For? Kausik Si Thaher Pelaseyed, Anthony Bretscher • Bacterial Chromosome Organization and Segregation, • Wnt-Frizzled/Planar Cell Polarity Signaling: Cellular Orientation by Facing the Wind (Wnt), Anjana Badrinarayanan, Tung B.K. Le, Michael T. Laub Yingzi Yang, Marek Mlodzik • Modulation of Host Cell Biology by Plant Pathogenic Microbes, • The Ins and Outs of Polarized Axonal Domains, Daniel R. Zollinger, Ruth Le Fevre, Edouard Evangelisti, Thomas Rey, Sebastian Schornack Kelli L. Baalman, Matthew N. Rasband • Assembly and Function of Spinal Circuits for Motor Control, • Ion Channels in Development and Cancer, Emily Bates Catarina Catela, Maggie M. Shin, Jeremy S. Dasen • Musashi Signaling in Stem Cells and Cancer, Raymond G. Fox, • Generating Neuronal Diversity in the Mammalian Cerebral Cortex, Frederick D. Park, Claire S. Koechlein, Marcie Kritzik, Tannishtha Reya Simona Lodato, Paola Arlotta • Mini-Gut Organoids: Reconstitution of Stem Cell Niche, Shoichi Date, • Monoallelic Expression of Olfactory Receptors, Toshiro Sato Kevin Monahan, Stavros Lomvardas • Genetics of Gonadal Stem Cell Renewal, Leah Joy Greenspan, • Development of Dendritic Form and Function, Margaret de Cuevas, Erika Matunis • Studying Lineage Decision-Making In Vitro: Emerging Concepts Julie L. Lefebvre, Joshua R. Sanes, Jeremy N. Kay • Sculpting Neural Circuits by Axon and Novel Tools, Stefan Semrau, Alexander van Oudenaarden and Dendrite Pruning, • Feeling Force: Physical and Physiological Principles Enabling Martin M. Riccomagno, Sensory Mechanotransduction, Samata Katta, Michael Krieg, Alex L. Kolodkin Miriam B. Goodman • Mechanotransduction's Impact on Animal Development, Evolution, and Tumorigenesis, Maria-Elena Fernandez-Sanchez, Thibaut Brunet, Jens-Christian Röper, Emmanuel Farge • Comparative Analysis of Gene Regulatory Networks: From Network Reconstruction to Evolution, Dawn Thompson, Aviv Regev, Sushmita Roy • The Developmental Control of Transposable Elements and the Evolution of Higher Species, Marc Friedli, Didier Trono
Access all Annual Reviews journals via your institution at www.annualreviews.org
ANNUAL REVIEWS | Spark a Connection
T: 650.493.4400/800.523.8635 (us/can) | [email protected] July 21–25, 2016, Prague, Czech Republic, www.iccb2016.org Prague Congress Centre
12th International Congress of Cell Biology
© CZECHTOURISM.COM © Exploring cellular structure and function
KEYNOTE LECTURES Di Fiore Pier Paolo – The Numb: p53 axis connects asymmetric cell division and tumor suppression in mammary stem cells Betzig Eric – Imaging life at high spatiotemporal resolution Zerial Marino – Molecular mechanisms of endosome biogenesis and fusion zur Hausen Harald – Bovine milk and meat as risk factors for human diseases Jacobs Howy – Maintenance of mitochondrial fidelity Cossart Pascale – What bacteria – in particular Listeria – have learnt to cell biologists Baumeister Wolfgang – Structural biology in a cellular context Simons Kai – The dynamic organisation of cell membranes Peters Jan-Michael – How cohesin controls sister chromatid cohesion and chromatin structure Chalfie Martin – Determining neuronal cell fate in C. elegans Heald Rebecca – Mechanisms of mitosis and size control in Xenopus Hirokawa Nobutaka – Kinesin superfamily molecular motors, KIFs: from regulation of neuronal functions and development to diseases Nigg Erich – Impact of centrosome aberrations on microcephaly and cancer Gall Joseph – Giant chromosomes and deep sequences: what the amphibian oocyte tells us about transcription Special lectures: Gasser Susan – Heterochromatin and its crucial role in genome maintenance Bertuzzi Stefano – The curious incident of the translational dog that didn’t bark. Where is cell biology going? And Goldman Robert – Intermediate filament networks are systems integrators in mammalian cells where a PhD in science take you? Walter Peter – From protein folding to cognition: the serendipitous path of discovery Mattaj Iain – Promoting excellence by choice; EMBL and international life science collaboration ABSTRACT SUBMISSION TOPICS – MINISYMPOSIA SPECIAL & MAIN SYMPOSIA LECTURES Cell biology of host-pathogen interactions (Peter Šebo + Pascale Cossart) Symposia lectures: Mechanisms of intracellular tracking (Daniela Corda + Margaret Robinson) McKnight Steven – A solid state conceptualization of information transfer from gene to message to protein Epigenetic control of cell fate (Guoliang Xu + Petra Hájková) Silver Pamela – Designing biology – from basic cell biology to application Microtubule organization in health and disease (Mónica Bettencourt-Dias + Pavel Dráber) Almouzni Geneviève – Shaping chromatin in the nucleus, key architects Lamins and chromatin during development and ageing (Roland Foisner + Yixiang Zheng) Hetzer Martin – How the nuclear membrane controls genome function Emerging technologies for cell biology (Alasdair Steven + Stanislav Kmoch) Misteli Tom – Deep imaging to see the genome DNA replication, repair and recombination (Cristina Cardoso + Eva Bártová) Bártek Jiří – Genome integrity maintenance: mechanisms and relevance for cancer and aging Pluripotent stem cells (Alexey Tomilin + Aleš Hampl) Wang Xiaodong – Biochemical pathways of cell death Chromosome segregation and aneuploidy (William Earnshaw + Iain Cheeseman) Yoshimori Tamotsu – Autophagy: its molecular basis and anti-disease function Nuclear periphery (Yosef Gruenbaum + Ohad Medalia) Vandenabeele Peter – Role of RIPKs in life and death decisions Shaping the cell (Buzz Baum + Tomáš Mazel) Leptin Maria – Subcellular and supracellular mechanisms of cell morphogenesis Host cells and vector born pathogens (Libor Grubho¤er + Marshall Bloom) Mayor Satyajit – Active control of cell membrane composition Tissue microenvironment under physiological condition and disease (Hernandes Carvalho + Karel Smetana) Humphries Martin – Regulation of cell adhesion through the cell cycle Neurons meeting glia in the brain plasticity, disease and therapy (Leszek Kaczmarek + Miloš Pěkný) Watt Fiona – Studying epidermal stem cells at single cell resolution Dynamics of the gene expression machinery (David Tollervey + Thoru Pederson) Grosveld Frank G. – Transcription and the dynamics of the 3D structure of the genome Centrioles, centrosomes and cilia: function and dysfunction (Jordan Ra¤ + Renata Basto) Martienssen Rob – Epigenetic reprogramming and small RNA Chromatin plasticity and nuclear architecture (Evi Soutoglou + Eran Meshorer) Lin Haifan – Uniting the genome: multifaceted roles of the Piwi-piRNA pathway in the germline Cell adhesion dynamics in tissue organization (Carien Niessen + Corinne Albigès-Rizo) Hořejší Václav – Immunoreceptor signalling Cell biology and nanomedicine (Gareth Gri©ths + Arwin Jones) LeRoith Derek – The role of GH/IGF-I axis in the regulation of glucose metabolism and its disturbances Plant development and adaptation (Jiří Friml + María-Carmen Risueño) Oren Moshe – The fifty shades of p53 Non-coding RNAs in health and disease (Claus Azzalin + Štěpánka Vaňáčová) Abstract submission deadline – March 30, 2016 Early Registration Deadline – April 30, 2016
Visit us at ASCB 2015 and learn more at the learning center – booth No. 944 Submit your abstract and Register online NOW ! More information at www.iccb2016.org
International Federation for Cell Biology
cscb2016-adv-8.875x11.375in-r03.indd 1 23. 10. 2015 14:27:02 Cellular and Molecular Gastroenterology and Hepatology
The newest member of the AGA’s family of journals! Elsevier is delighted to bring you a new journal from the American Gastroenterological Association (AGA), Cellular and Molecular Gastroenterology and Hepatology (CMGH). CMGH reports the latest advances in cell biology, immunology, physiology, microbiology, genetics, and neurobiology of gastrointestinal, hepatobiliary, and pancreatic health and disease. In an effort to distribute its content to the widest audience possible, CMGH is an all-digital, open access journal.
Online Article Submission Authors interested in submitting articles to CMGH should visit www.editorialmanager.com/cmgh. Some of the benefits for authors include: Edited by: • Articles published in CMGH will be immediately free to read by academics, researchers, and the Jerrold R. Turner, MD, PhD, AGAF public throughout the world, without restriction James R. Goldenring, MD, PhD, AGAF • Rigorous peer review by experts in the field Rebecca G. Wells, MD • Rapid online publication • Choice of Creative Commons licenses, determining how you want your work to be used • Community connections—share your research with your peers around the world CMGHjournal.org Submit your manuscript at www.editorialmanager.com/cmgh.
OFFICIAL JOURNAL OF THE AGA INSTITUTE Contents
Meeting at a Glance...... 2 Professional Development Programs at a Glance...... 5 Education Programs at a Glance...... 6 ASCB Annual Meeting Supporters...... 7 ASCB Corporate Members ...... 8 ASCB Council, Staff, and Committees...... 9
Attendee Resources Registration Information ...... 11 Learning Center (Exhibit Hall) Information...... 11 Attendee and Convention Center Information...... 12 Speaker Resources...... 15 Accessibility/Disabilities Information...... 15 Meeting Policies ...... 15 Hotel Information ...... 17 Safety and Emergency Information...... 17 Convention Center Maps ...... 18
ASCB Awards Education Committee Travel Awards ...... 20 ASCB Childcare Awards ...... 21 ASCB Minorities Affairs Committee Travel Awards ...... 22 International Travel Awards...... 22
Scientific Program Saturday Program...... 25 Sunday Program...... 41 Monday Program...... 71 Tuesday Program ...... 101 Wednesday Program...... 127
Exhibitors Learning Center (Exhibit Hall) Floor Plan ...... 138 Exhibitors...... 140 Exhibitor Product Index...... 155
Abstract Author Index (Oral Presentations)...... 165 Abstract Author Disclosures...... 172
Note The poster listings and poster author index are in a separate publication, the Poster Guide.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 1 MEETING AT A GLANCE Saturday Sunday
8:00 am Registration Open 7:30 am Registration Open Exhibitor Tech Talk Career Center Open Bruker Nano Surfaces: Advances in live super- 9:00 am Mentoring Keynote resolution imaging using the Vutara 352 8:00 am Symposium 1 microscope 10:30 am Grant Writing Seminar Pushing the Limits: Visualization of Hidden Biological Processes 1:00 pm Exhibitor In-Booth Presentation 1:00 pm Special Interest Subgroups Wikipedia Edit-a-Thon National Institutes of Health (NIH): National A. Autophagy in Disease and Survival Institute of General Medical Sciences (NIGMS) B. Building the Cell 9:00 am Delivering Science: Effective Communication and Center for Scientific Review (CSR) C. Cellular and Molecular Mechanobiology: Skills to Become a Successful Scientist Film: The Biology of Skin Color New Approaches, Systems, and Responses D: Connexins and Pannexins in Disease 9:30 am ASCB Learning Center (Exhibit Hall) Open 1:15 pm Exhibitor In-Booth Presentation E: Cytoskeletal and Membrane Protein Table Talk Abcam, Inc: Immunostaining: From Sample Prep Dynamics at the T Cell Immunological Strategies for Teaching Science Writing and through Troubleshooting and Beyond Synapse Literature Reading Exhibitor In-Booth Presentation F: Diverse Roles of Glycans and Glycan- Exhibitor Tech Talk Photometrics: Imaging with signal restoration Binding Proteins in Human Diseases Nanolive SA: Nanolive launches the first super powers G: Dynamic Interplay between Lipids, holographic tomographic microscope in the Curvatures, and Diseases of Biological world: the 3D Cell Explorer 1:25 pm Microsymposium 3: Membrane Dynamics and Membranes Lipotype GmbH: An automated shotgun Visualization H: Extracellular Vesicles - Biogenesis and lipidomics platform for high Microsymposium 4: Cell Division and Function throughput, comprehensive, and Cytokinesis I: Increasing Diversity in a Changing Research quantitative analysis of lipids from Landscape biological and clinical samples 1:30 pm Meet the Editor of CBE—Life Sciences Education J: Microtubule Networks in Differentiated Open Imaging, Inc.: Open source image Table Talk Cells acquisition with μManager Incorporating Research into Lab Courses— K: Neuronal Cytoskeleton: Cytoarchitecture Exhibitor Tech Talk Reports from REIL Biology and Dynamics Cell Signaling Technology: Simple assays for Table Talk L: Nuclear Envelope Dynamics cellular analyses Using Cutting-Edge Models in the Classroom M: Nucleation Phenomena in Cell Biology Morning Refreshment Break Even-Numbered Poster Presentations N: Polymerizing Enzymes: New Frontiers in Protein Compartmentalization 2:00 pm Exhibitor In-Booth Presentation and Localization 9:45 am Symposium 2 Nanomedical Diagnostics: AGILE Research O: Quantitative Microscopy and Image Wisdom of Crowds: Collective Decision- Biosensor live demonstration with Analysis: Measuring Cellular Making by Cells and Organisms Organization and Dynamics complimentary beer 10:00 am Foundational Cell Biology Workshop: Making International Affairs Committee (IAC) Roundtable 2:15 pm Undergraduate Program BIG Data Accessible for Teaching and Learning Exhibitor Tech Talk Nikon Instruments, Inc.: Illuminating biology 3:30 pm Poster Competition and Reception 10:30 am Making the Most of the Annual Meeting with super-resolution microscopy Career Panel: Science Policy 4:00-5:30 pm High School Program 10:45 am Exhibitor Tech Talk Exhibitor In-Booth Presentation Explore the World with a Fold-Your-Own eBioscience, an Affymetrix Business: Single- QImaging: You can have it all: new cameras, new Microscope cell gene expression in context advanced features, new software Exhibitor Tech Talk 5:00 pm Meet and Greet Thermo Fisher Scientific: New transfection 2:50 pm Microsymposium 5: Mechanics in Cellular reagents for CRISPR editing and in vivo Maintenance and Disease 6:00 pm Keynote Symposium applications Microsymposium 6: Studying Organelle Function: Sallie W. Chisholm, Massachusetts New Trends and Technologies Institute of Technology 11:00 am Talking about Evolution with Doubters: Practical The Honorable, Professor Jane Lubchenco, Tips 3:00 pm Science Discussion Tables Oregon State University Exhibitor In-Booth Presentation Exhibitor Tech Talk Posters on Display National Institutes of Health (NIH): National Bitplane Inc.: Cell lineage analysis – Institute of General Medical Sciences (NIGMS) Imaris 8.2 launch at ASCB 2015 Immediately Opening Night Reception and Center for Scientific Review (CSR) WICB Network Reception Goes International following Science Discussion Tables Career Panel: Consulting and Entrepreneurship Keynote- E.E. Just Lecture HHMI Biointeractive Animations 10:00 pm Erich D. Jarvis Exhibitor In-Booth Presentation 3H Biomedical AB: Characterization and large 8:00 pm International Research and Training 12:00 pm Odd-Numbered Poster Presentations scale expansion of human satellite cells Exchange Fair Microsymposium 1: Cell Motility and Migration Large-Scale Data Workship: Computational Microsymposium 2: Signaling in Health and Methods for RNA Sequencing Analysis Disease Exhibitor Tech Talk 3:15 pm Bruce Alberts Award for Excellence in Science VitaCyte: Collagenase: moving from black box Education to transparent knowledge Deborah Harmon Hines Exhibitor Tech Talk EMD Millipore: Cell cycle and cell death: 4:00-6:25 pm Multicellular Interactions, Tissues, and studying the connections with simplified Development cytometry Minisymposium 1: Cell Migration in Tissues MD-PhD, Is It Right for Me? Signaling and Differentiation Table Talk Minisymposium 2: Cellular Decision-Making Foldscopes Cell Cycle, and Cell Division Afternoon Refreshment Break Minisymposium 3: Chromosome Segregation Cell Biology of Genetic Information 12:30 pm Table Talk Minisymposium 4: Genome Organization and Lessons Learned about Starting a New Lab Stability Table Talk Membrane Organization, Dynamics, Traffic, and National Research Mentoring Network Regulation Exhibitor In-Booth Presentation Minisymposium 5: Mechanisms for Shaping QImaging: You can have it Membranes all: new cameras, new advanced Cytoskeleton, Motility, and Cell Mechanics features, new software Minisymposium 6: Molecular Motors and the Cytoskeleton: Measurement, Manipulation, 1:00 pm Startups and Scientists: Our Strengths and and Mechanics Weaknesses as Entrepreneurs Education Minisymposium: Teaching How to Teach Exhibitor Tech Talk and Learn Carl Zeiss Microscopy, LLC: Accessing the emerging imaging technologies at HHMI 4:15-5:15 pm Exhibitor Tech Talk Janelia Research Campus Horizon Discovery: Genome editing in human Carl Zeiss Microscopy, LLC: New acquisition cells using CRISPR/Cas technology and detection modes with ZEISS Airyscan Carl Zeiss Microscopy, LLC: AiryScan: bringing 6:45 pm ASCB Kaluza Prize Presentation and Keith R. Porter super resolution to confocal microscopy Lecture by Jonathan S. Weissman
2 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting MEETING AT A GLANCE Monday
7:30 am Registration Open 1:25 pm Membrane Organization, Dynamics, Traffic, and Career Center Open Regulation Microsymposium 9: Membrane Trafficking 8:00 am Symposium 3 Applications of Cell Biology in the Real World Embraces across the Species Barrier: Microsymposium 10: Applications of Cell Complex Cell Interactions Biology Learning Center in the Real World
8:15 am Exhibitor Tech Talk 1:30 pm Meet the Editor of Molecular Biology of the Cell Leica Microsystems: Infinite possibilities in Even-Numbered Poster Presentations ASCB live cell imaging: advances in confocal and widefield imaging by Leica Microsystems 2:00 pm Table Talk Exhibitor Tech Talk Teaching and Research at a Small, Liberal Arts TESCAN USA, INC.: Digital holographic College Learning Center Annual microscopy: a revolutionary approach for Exhibitor Tech Talk visualizing cellular morphology in real time Cellecta Inc.: CRISPR and RNAi: gene-editing and functional enomicg screening approaches Meetings 9:00 am Green Cards for Scientific Researchers: U.S. Exhibitor In-Booth Presentation Immigration Options QImaging: You can have it all: new cameras, new advanced features, new software 9:30 am ASCB Learning Center (Exhibit Hall) Open Exhibitor Tech Talk Morning Refreshment Break EMD Millipore: Winning Westerns for cell Table Talk biologists: get reliable expression data from PALM Network lysates Exhibitor Tech Talk Career Panel: Science Communication Bruker Nano Surfaces: Opterra II: 4D confocal 2016 imaging at the speed of life 2:45 pm Exhibitor In-Booth Presentation Exhibitor Tech Talk Abcam, Inc: Immunostaining: From sample San Francisco GE Healthcare: Advances in design for prep through troubleshooting and beyond increased specificity and function in CRISPR/ December 3–7 CAS9 and shRNA based gene modulation 2:50 pm Organelles and Spatial Organization of the Cell Microsymposium 11: Nucleus Biology and 9:45 am Symposium 4: Like Oil and Water: New Disease Learning Center Principles Governing Cell Organization Signaling and Differentiation Microsymposium 12: Signaling in 2017 10:00 am COMPASS Open Forum Differentiation and Cancer Simple Social Media for Scientists Philadelphia 3:00 pm Science Discussion Tables 10:30 am Advocacy Toolbox: The Two-Minute Speech Career Panel: Teaching and University Administration December 2–6 10:45 am Career Discussion and Mentoring Roundtables Exhibitor Tech Talk Exhibitor Tech Talk ALVÉOLE: Multi-protein printing by light- Wiley: Open Access in publishing induced molecular adsorption: application to Exhibitor Tech Talk cell biology 2018 Etaluma, Inc.: Enabling low cost drug 3Is in South Korea: Initiatives, Innovations, and discovery with genetically encoded Information for Bioscience San Diego biosensors and “next generation” imaging Exhibitor In-Booth Presentation National Institutes of Health (NIH): National December 8–12 11:00 am Answers to Questions about Peer Review Institute of General Medical Sciences (NIGMS) Fast-Growing Cell Biology Society in China and Center for Scientific Review (CSR) Exhibitor In-Booth Presentation 12:00 pm Odd-Numbered Poster Presentations 3H Biomedical AB: Characterization and large Cytoskeleton, Motility, and Cell Mechanics scale expansion of human satellite cells 2019 Microsymposium 7: Microtubule Dynamics: Exhibitor Tech Talk From +TIPs to Membrane BioTek Instruments: Kinetic live cell imaging Boston Multicellular Interactions, Tissues, and Large-Scale Data Workshop: Quantitative Analysis Development and Visualization of Signaling Networks November 18–20 Microsymposium 8: The Role of the Cytoskeleton in Disease and Repair 3:15 pm Education Initiative Forum Programmatic Efforts at NIH to Promote and Integrating Discovery-Based Research into the Support the Careers of Women in Biomedical Undergraduate Curriculum: Report on an NAS Science Convocation Exhibitor Tech Talk GE Healthcare: Live cell structured 4:00 pm Networking Happy Hour illumination imaging: a new reality Kaluza Minisymposium Afternoon Refreshment Break Cell Cycle and Cell Division Minisymposium 7: Centrosomes and Spindles 12:15 pm Exhibitor In-Booth Presentation Membrane Organization, Dynamics, Traffic, and Abcam, Inc: Immunostaining: From sample Regulation prep through troubleshooting and beyond Minisymposium 8: Lipid Organization, Transport,Composition, and Phosphoinositides 12:30 pm Table Talk Cytoskeleton, Motility, and Cell Mechanics Council on Undergraduate Research Minisymposium 9: Microtubule-Based Motility Exhibitor In Booth Presentation and Dynamics Nanomedical Diagnostics: AGILE Research Signaling and Differentiation Important Numbers Biosensor live demonstration with Minisymposium 10: New Technologies and complimentary beer Immuno-Signaling Cell Biology of Genetic Information Attendee/ Membership Services 1:00 pm MALT Workshop Minisymposium 11: Nuclear Mechanics and 619-525-6202 Reforming Undergraduate STEM Education Transport through Long-Term Mentorships: The ASCB Organelles and Spatial Organization of the Cell Mentoring in Active Learning and Teaching Minisymposium 12: Organelle Dynamics, ASCB Meeting Management (MALT) Program Structure, and Function 619-525-6200 Exhibitor Tech Talk ACEA Biosciences: Use of real time cellular 4:15 pm Exhibitor Tech Talk analysis and flow cytometry to characterize Applied Scientific Instrumentation Inc.: ASCB Exhibit Management potential biomarkers of aggressive colorectal Advances in dual inverted selective plane 619-525-6209 cancer microscopy (diSPIM) and laser technology Exhibitor Tech Talk Exhibitor Tech Talk Thermo Fisher Scientific: Engineering NanoSurface Biomedical, Inc.: Nano- Lost & Found fluorescence for cell biology engineering cell phenotype and Career Options and Job Resources for Scientists function with aligned nanopatterned cell 619-525-6200 Exhibitor In-Booth Presentation culture dishes National Institutes of Health (NIH): National Montana Molecular: Single fluorescent protein- Institute of General Medical Sciences based biosensors for detecting Gi, Gs, and First Aid (NIGMS) and Center for Scientific Review Gq-coupled pathways of GPCR signaling 619-525-6212 (CSR) VitaScientific: New tools for cell biology from VitaScientific.com 1:15 pm Exhibitor In-Booth Presentation In an emergency use the nearest Photometrics: Imaging with signal restoration 5:30 pm Exhibitor Tech Talk beige house phone and dial x5911. super powers Nanolive SA: Product Launch: 3D Cell Explorer, revolutionary microscope able to image living cells instantly, in 3D and 4D
6:45 pm Emerging Topic Symposium - Mitochondria and Cancer Cell Biology
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 3 MEETING AT A GLANCE
Tuesday Wednesday
7:30 am Registration Open and characterization by analytical ultra- 7:30 am Registration Open Career Center Open centrifugation and dynamic light scatter Exhibitor Tech Talk 8:30 am Cytoskeleton, Motility, and Cell Mechanics 8:00 am Symposium 5: Bending Nature to Our Purposes: Andor: Fast and sensitive camera Minisymposium 19: A Tribute to Alan Hall: Engineering of Cells and Tissues technologies for microscopy Rho GTPase Signaling Exhibitor In-Booth Presentation 8:15 am Exhibitor Tech Talk National Institutes of Health (NIH): National Applications of Cell Biology in the Real World Sciencell Research Laboratories: Maximize Institute of General Medical Sciences Minisymposium 20: Applications of Cell your cell culture: technical tips for primary (NIGMS) and Center for Scientific Review Biology 2 cells, pluripotent stem cells, and cell analyses (CSR) Cell Cycle and Cell Division 9:30 am ASCB Learning Center (Exhibit Hall) Open 1:25 pm Microsymposium 15: Membrane Regulation and Minisymposium 21: Cell Cycle Regulation Morning Refreshment Break Signaling Signaling and Differentiation Exhibitor Tech Talk Microsymposium 16: Cell Biology of Genetic Minisymposium 22: Mammalian Cell Reveal Biosciences: Quantitative multiplexed Information Signaling in situ hybridization (ISH) in tissue and cell- based assays 1:30 pm Table Talk Cytoskeleton, Motility, and Cell Mechanics One World Lab: New technologies to assess Crafting a Research Statement That Is Undergraduate Research Friendly Minisymposium 23: Motility and quality and compatibility of commercial Cytoskeleton of Microbes antibodies from multiple vendors Even-Numbered Poster Presentations EditBIOMED: What can a scientific editing Membrane Organization, Dynamics, Traffic, and service do for you? 2:00 pm Career Panel: Industry and Sales Exhibitor Tech Talk Regulation Minisymposium 24: New Insights Into Thermo Fisher Scientific: Automated protein 9:45 am Symposium 6: Going the Distance: Determining Secretory Trafficking Mechanisms detection -the future of Westerns Size and Spacing of Biological Structures Exhibitor Tech Talk Organelles and Spatial Organization of the Cell Essential Pharmaceuticals: Variables in FBS 10:00 am One-Minute, All-Video Elevator Speech Contest Minisymposium 25: Organelle Homeostasis impact cell performance, reproducibility, and Table Talk and Turnover Transitioning to a Postdoc Position relevant experimental results Multicellular Interactions, Tissues, and 10:30 am Building a Successful Research Program with 2:50 pm Microsymposium 17: Spindle Assembly and Development Undergraduates—A Roadmap Chromosome Dynamics Minisymposium 26: Tissue Biology Microsymposium 18: Cell Mechanics and Adhesion 10:45 am Exhibitor Tech Talk 11:15 am Symposium 7 Wiley: Want people to read your paper? 3:00 pm Celldance Videos, Elevator Speech Contest Finals, Beyond the Five Senses: Detection of Here’s how to optimize your chances… and Comic Contest Awards Magnetic and Electric Fields Exhibitor Tech Talk Exhibitor Tech Talk Tecan: Rapid generation of compound serial Essen BioScience: Novel kinetic live cell imaging 12:15 pm Meeting Ends dilutions orf cell assays using an inkjet based assays for T cell killing of tumor cells dispensing technology Research in Cell Biology in France: Opportunities Women in Cell Biology (WICB) Committee and Tips Awards Presentation and Career Panel: Research Development and Grant Mentoring Theater: Who Me? I’m Not Biased. Administration Embracing Diversity to Improve Creativity Science Discussion Tables Morning with the Editors: An Open Discussion ASCB Business Meeting with Journal Editors Exhibitor In-Booth Presentation National Institutes of Health (NIH): National 11:00 am Advocacy Toolbox: How to Start and Sustain a Institute of General Medical Sciences (NIGMS) Policy Advocacy Group and Center for Scientific Review (CSR) LGBTQ Diversity Session Large-Scale Data Workshop: Image Analysis in Brazil: Opportunities for the Young: Fellowships, Quantitative Microscopy Resources, and Interactions Subgroup P: The Cellular and Molecular Basis of Table Talk Invasive Metastatic Cancer Bringing Research into the Undergraduate Curriculum Learning Center 3:15 pm Education Initiative Forum Using Visual Networks to Map the Systems 11:30 am Table Talk Thinking Literature Developing Case Studies in Cell Biology 3:30 pm POSTER REMOVAL 12:00 pm Exhibitor Tech Talk EMD Millipore: New advances in microfluidic 4:00 pm Applications of Cell Biology in the Real World control of cellular microenvironment with Minisymposium 13: Applications of Cell Biology 1 uninterrupted imaging allows for highly Cell Cycle and Cell Division controllable, long-term, more in vivo-like cell Minisymposium 14: Cytokinesis culture studies Membrane Organization, Dynamics, Traffic, and Odd-Numbered Poster Presentations Regulation Microsymposium 13: Morphology of the Minisymposium 15: Endo-Lysosome Trafficking Cytoskeleton Leading toMorphology in in Development and Disease Development Multicellular Interactions, Tissues, and Microsymposium 14: Actin Cytoskeleton Development Dynamics Minisymposium 16: Morphogenesis Exhibitor In-Booth Presentation Organelles and Spatial Organization of the Cell Abcam, Inc: Immunostaining: From sample Minisymposium 17: New Technologies and Their prep through troubleshooting and beyond Application to Probe the Spatial Organization of Afternoon Refreshment Break the Cell Exhibitor Tech Talk Cytoskeleton, Motility, and Cell Mechanics See you next GE Healthcare: Quantitative Western blotting: Minisymposium 18: Regulation and Integrated improving your data quality and Functions of Actin Cytoskeleton year in reproducibility 6:45 pm ASCB-Gibco Emerging Leader Prize Presentation 1:00 pm Navigating the New NIH Biosketch and E.B. Wilson Medal Presentation by Elaine San Francisco, Exhibitor Tech Talk Fuchs Beckman Coulter Life Sciences: Extracellular vesicle isolation by flow cytometric sorting Dec. 3–7!
Note: The Exhibit Hall has been renamed the ASCB Learning Center
4 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting professional development At a glance Saturday Monday
9:00-10:15 am Mentoring Keynote 1:00-1:55 pm Career Options and Job Resources for Scientists
10:30 am-2:30 pm Grant Writing Seminar 1:00-2:00 pm Exhibitor In-Booth Presentation National Institutes of Health (NIH): National 3:30-5:30 pm Poster Competition and Reception Institute of General Medical Sciences (NIGMS) and Center for Scientific Review 8:00-9:00 pm International Research & Training Exchange Fair (CSR) Sunday 2:00-2:55 pm Career Panel: Science Communication
7:30 am-8:00 pm Career Center Open 3:00-3:50 pm Science Discussion Tables 9:00-10:25 am Delivering Science: Effective Communication 3:00-3:55 pm Career Panel: Teaching and University Skills to Become a Successful Scientist Administration
9:30-10:30 am Table Talk 3:00-4:00 pm 3Is in South Korea: Initiatives, Innovations, and Strategies for Teaching Science Writing and Information for Bioscience Literature Reading Exhibitor In-Booth Presentation National Institutes of Health (NIH): National 10:30-11:25 am Making the Most of the Annual Meeting Institute of General Medical Sciences (NIGMS) and Center for Scientific Review (CSR) 11:00 am-12:00 pm Exhibitor In-Booth Presentation National Institutes of Health (NIH): National 4:00-6:00 pm Networking Happy Hour Institute of General Medical Sciences (NIGMS) and Center for Scientific Review (CSR) Tuesday Science Discussion Tables 7:30 am-8:00 pm Career Center Open 12:00-12:55 pm MD-PhD, Is It Right for Me?
12:30-1:30 pm Table Talk 10:45-12:00 pm Women in Cell Biology (WICB) Committee Lessons Learned about Starting a New Lab Awards Presentation and Mentoring Theater: Who Me? I’m Not Biased. 1:00-1:55 pm Startups and Scientists: Our Strengths and Embracing Diversity to Improve Creativity Weaknesses as Entrepreneurs 11:00 am-12:00 pm Advocacy Toolbox: How to Start and Sustain a 1:00-2:00 pm Exhibitor In-Booth Presentation Policy Advocacy Group National Institutes of Health (NIH): National LGBTQ Diversity Session Institute of General Medical Sciences Brazil: Opportunities for the Young: Fellowships, (NIGMS) and Center for Scientific Review Resources, and Interactions (CSR) 1:00-1:55 pm Navigating the New NIH Biosketch 2:00-2:55 pm Career Panel: Science Policy 1:00-2:00 pm Exhibitor In-Booth Presentation 3:00-3:50 pm Science Discussion Tables National Institutes of Health (NIH): National WICB Network Reception Goes International Institute of General Medical Sciences Career Panel: Consulting and Entrepreneurship (NIGMS) and Center for Scientific Review (CSR)
2:00-2:55 pm Career Panel: Industry and Sales
3:00-4:00 pm Research in Cell Biology in France: Monday Opportunities and Tips Career Panel: Research Development and Grant 7:30 am-8:00 pm Career Center Open Administration Science Discussion Tables 9:00-10:00 am Green Cards for Scientific Researchers: U.S. Immigration Options
10:00-11:00 am COMPASS Open Forum Simple Social Media for Scientists
10:30 am-12:00 pm Advocacy Toolbox: The Two-Minute Speech
10:45 am-12:00 pm Career Discussion and Mentoring Roundtables “ASCB,” “The American Society for Cell Biology,” “iBioSeminars,” 11:00 am-12:00 pm Answers to Questions about Peer Review and “Molecular Biology of the Cell” are registered trademarks Fast-Growing Cell Biology Society in China of The American Society for Cell Biology . “The Cell: An Image Library” is a common law trademark of The American Society 12:00 pm-12:55 pm Programmatic Efforts at NIH to Promote and for Cell Biology . Support the Careers of Women in Biomedical Science
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 5 Education PROGRAMS AT A GLANCE
Saturday Monday 2:15-3:15 pm Undergraduate Program 9:30-10:00 am Table Talk PALM Network 4:30-5:30 pm High School Program: 12:30-1:30 pm Table Talk Explore the Microscopic World with Your Own Council on Undergraduate Research Foldscope 1:00-2:00 pm MALT Workshop Sunday Reforming Undergraduate STEM Education through Long-Term Mentorships: The ASCB Mentoring in Active Learning and Teaching 10:00-12:00 pm Foundational Cell Biology Workshop: Making (MALT) Program BIG Data Accessible for Teaching and Learning
2:00-3:00 pm Table Talk 11:00 am-12:00 pm Talking about Evolution with Doubters: Practical Teaching and Research at a Small, Liberal Arts Tips College 12:00–1:00 pm Table Talk 3:15-3:45 pm Education Initiative Forum Foldscopes Integrating Discovery-Based Research into the Undergraduate Curriculum: Report on an NAS 12:30-1:30 pm Table Talk Convocation National Research Mentoring Network
1:00-1:20 pm Film: The Biology of Skin Color Tuesday
1:30-2:30 pm Table Talk 10:30 am-12:30pm Building a Successful Research Program with Incorporating Research into Lab Courses— Undergraduates—A Roadmap Reports from REIL Biology Table Talk 11:00 am-12:00 pm Table Talk Using Cutting-Edge Models in the Classroom Bringing Research into the Undergraduate Meet the Editor of CBE—Life Sciences Education Curriculum
3:00-4:00 pm HHMI Biointeractive Animations 11:30 am-12:30 pm Table Talk Developing Case Studies in Cell Biology 3:15-3:45 pm Bruce Alberts Award for Excellence in Science Education 1:30-2:30 pm Table Talk Deborah Harmon Hines Crafting a Research Statement That Is Undergraduate Research Friendly 4:00 pm-6:25 pm Education Minisymposium: Teaching How to Teach and Learn 3:15-3:45 pm Education Initiative Forum Using Visual Networks to Map the Systems Thinking Literature
6 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting The ASCB thanks the following organizations for supporting the 2015 ASCB Annual Meeting.*
Atlas Antibodies AB International Federation for Cell Biology (IFCB) Enhanced Company Listing on Mobile App Morning with the Editors Session
BioMed Central John Wiley & Sons Ltd. Minisymposium Talk Minisymposium Talk Symposia Talks Bruker Hanging Banner Aisle Sign Journal of Molecular Cell Biology Registration Area Pens and Note Pads Burroughs Wellcome Fund Career Discussion Tables The Journal of Cell Biology Special Interest Subgroups Minisymposium Talk MAC Travel Awards Mentoring Keynote Lipotype GmbHe Enhanced Company Listing on Mobile App Chroma Technology Corporation Travel Awards National Cancer Institute, NIH Emerging Topic Symposium The Company of Biologists Travel Awards Nature Publishing Group Childcare Awards EMD Millipore Hotel Room Keys Olympus America Hanging Banner Aisle Sign Lanyards
Essen BioScience Photometrics Enhanced Company Listing on Mobile App Abstract Submission System
Genentech PROTEINSIMPLE General Meeting Support Enhanced Company Listing on Mobile App
Getson & Schatz, P.C. Sanofi Travel Awards Symposium Talk Meeting Registrations Hamamatsu Corporation Large Scale Data Workshop SGI-DNA, A Synthetic Genomics Company Symposium Talk Howard Hughes Medical Institute E.E. Just Award Lecture Simons Foundation Travel Awards supporting Visiting Professor Program Wikipedia Edit-a-Thon Foundational Cell Biology Workshop Society for Neuroscience International Centre for Genetic Engineering and Enhanced Company Listing on Mobile App Biotechnology (ICGEB) Travel Awards Worthington Biochemical Corporation Travel Awards *As of November 5, 2015 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 7 The ASCB is grateful to its 2015 Corporate Members
Gold Allen Institute for Cell Science Beckman Coulter Life Science EMD Millipore Genentech Getson & Schatz, P.C. Nikon Instruments, Inc ScienCell Research Laboratories Thermo Fisher Scientific
Silver Chroma Technology Corporation
Bronze Cosmo Biotechnologies Ltd Okolab SRL Olympus
8 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting THE AMERICAN SOCIETY FOR CELL BIOLOGY executive committee President Shirley Tilghman, Princeton University President-Elect Peter Walter, University of California, San Francisco School of Medicine President-Elect Designate Pietro De Camilli, Yale University School of Medicine Past President Jennifer Lippincott-Schwartz, Bethesda, MD Secretary Kathleen Green, Northwestern University Feinberg School of Medicine Treasurer Gary Gorbsky, Oklahoma Medical Research Foundation Executive Director, ex officio Stefano Bertuzzi, American Society for Cell Biology COUNCIL Martin Chalfie, Columbia University Ira Mellman, Genentech, Inc. Anthony A. Hyman, Max Planck Institute of Tom Misteli, Bethesda, MD Molecular Cell Biology and Genetics Denise J. Montell, University of California, Santa Barbara Daniel P. Kiehart, Duke University Jodi Nunnari, University of California, Davis Ruth Lehmann, New York University Medical Center/HHMI Samara Reck-Peterson, University of California, San Diego Ian G. Macara, Vanderbilt University Medical Center Claire Walczak, Indiana University Laura M. Machesky, CRUK Beatson Institute for Cancer Research ASCB Staff Eric Baker, MBoC Journal Production Manager John Fleischman, Senior Science Writer Mark Leader, Director of Publications and Newsletter Shannon Behrman, iBiology Associate Director Cynthia Godes, Senior Director of Finance & Editor Justin Brantley, IT Support Specialist Administration Cheryl Lehr, Executive Assistant/Office Manager Louise Campbell-Blair, Director, Business Development Sarah Goodwin, iBiology Director Lynn Marquis, Coalition for the Life Sciences Director Marta Chacon, Membership Manager Alison Harris, Interim Associate Director of Mike McCormack, Chief Technology Officer Johnny Chang, Production and Marketing Manager Meetings Kelly McManus, Accounting Manager Kam Fei (Winny) Cheng, Accounting Assistant Eric Kornblum, iBiology Videographer Desirée Salazar, Scientific Program Manager Thea Clarke, Director of Communications and Education Martin Koslof, .NET Developer Christina Szalinski, Science Writer/Program Coordinator Armand Davila, Web Developer Heather Kyler, Meeting Manager Committees Education International Affairs Anthony M. Moss Scott Wilkinson Carol W. Greider Susan M. Wick, Chair Yixian Zheng, Chair Dennis Zimmermann Jennifer Lippincott-Schwartz Merri Lynn Casem Ranan Gulhan Aktas Minorities Affairs Jodi Nunnari Edward Cluett Kirk William Deitsch Renato J. Aguilera, Co-Chair Program Committee Laura Pajak Lena Diaw Karen R. Dell Andrew G. Campbell, Co-Chair Julie A. Theriot, Chair Shirley M. Tilghman Megan Dobro William C. Earnshaw David R. Burgess Steven J. Altschuler Susan Rae Wente Joyce J. Fernandes Xin-Hua Feng Franklin A. Carrero-Martinez Angelika Amon Mark Winey Tracie M. Gibson Celia Regina Da Silva Garcia Benjamin L. Clarke Kerry S. Bloom Liaisons Daniel John Goduti Satyajit Mayor Luis Cubano Christopher S. Chen Simon J. Atkinson Jennifer Katharine Hood-DeGrenier John A. Mercer Latanya P. Hammonds-Odie David Gil Drubin Kellyann Nadira Jones-Jamtgaard Melissa Kleinschmit James M. Ntambi Michael J. Leibowitz Hana El-Samad Tony J. Koleske Tony J. Koleske David S. Roos Brian Lewis Wendell A. Lim Tim Stearns Patrick Lajoie David Andrew Shifrin Sandra Ann Murray Melissa Moore Beverly Rogers Wendland Irina Makarevitch Xuebiao Yao Veronica A. Segarra Sarah E. Rice Ex Officio Mark Peifer Associates Teresa Inez Shakespeare Avital A. Rodal Stefano Bertuzzi Amy Jeanette Prunuske Abel Rodrigo Alcazar-Roman Winston E. Thompson Jennifer Roecklein-Canfield Douglas E. Koshland Omar A. Quintero Seth Corey JoAnn Trejo David S. Roos Thomas D. Pollard Cheston Andrew Saunders Lina Dagnino Graciela A. Unguez Shirley M. Tilghman Janet M. Shaw Shannon Seidel Philip Richard Dash Leticia R. Vega Eric Wieschaus Brian Storrie Jenny Sider Michael Robert Dores MariaElena B. Zavala Alpha S. K. Yap Michael J. Wolyniak Debnath Ghosal Liaison Richard Youle Women in Cell Biology Associates Ghanshyam Das Heda Kellyann Nadira Jones-Jamtgaard Hong Zhang Sandra K. Masur, Chair Alexandra M. Ainsztein Eric Hwang Ex Officio Ex Officio Debra Page Baluch Meg Bentley Cynthia G. Jensen David J. Asai Stefano Bertuzzi Diane L. Barber Stephanie D. Carmicle-Davis Hideko Kaji Stefano Bertuzzi Julie A. Brill Scott R. Gehler Sophie A. Lelievre Tama Hasson Public Information Paula A. Bubulya Jennifer Lynne Hurst-Kennedy Dorothy Lerit Simon J. Atkinson, Chair Mary Dasso Lalitha Jayant Guangshuo Ou Committee for Postdocs and Students Scott D. Blystone Phyllis Ida Hanson Leocadia Victoria Paliulis Noreen Elizabeth Reist (COMPASS) Stanley A. Cohn Rebecca Heald Amy Rice Doetsch Jayanthi Repalli Bruno Da Rocha-Azevedo, Co-Chair Duane A. Compton Triscia Wharton Hendrickson Melanie L. Styers V. Kenyi Saito-Diaz Theodore Terence Ho, Co-Chair Victor Faundez Sophie G. Martin Liaison Marisa Segal Adriana S. Bankston Cristy Lee Gelling Mary Munson Scott Wilkinson Arpita Sen Brittany Jo Belin Sriparna Ghosh W. James Nelson Ex Officio Sara Szuchet Jay Manoj Bhatt Elisa M. Konieczko Tabitha Ann Peterson Shannon Behrman Justin W. Taraska Arunika Das Manuela Martins-Green Sowmya Swaminathan Stefano Bertuzzi Liaison Pinar Gurel Gavin Peter McStay Angela Wandinger-Ness Erin L. Dolan Dennis Zimmermann Kellyann Nadira Jones-Jamtgaard Rita K. Miller Ora A. Weisz Sarah Goodwin Ex Officio Gary Steven McDowell Brad J. Nolen Beverly Rogers Wendland Katherine T. Schmeidler-Sapiro Stefano Bertuzzi Paul T. Mungai James Arthur Olzmann Associates Judith Kimble Melissa C. Pamula Nava Segev Elizabeth Marincola Finance & Audit Jessica K. Polka Jagesh V. Shah Sabine Petry Gary J. Gorbsky, Chair Membership V. Kenyi Saito-Diaz Claire E. Walczak Jennifer Roecklein-Canfield Magdalena Bezanilla Kathleen J. Green, Chair Elisa Vitiello Liaison Katherine T. Schmeidler-Sapiro James I. Garrels Andrew Jon Holland Lesley Nicole Weaver Pinar Gurel Liaisons Benjamin S. Glick Geri Kreitzer Associates Ex Officio Brittany Jo Belin Theodore Terence Ho Zu-Hang Sheng Travis J. Bernardo Stefano Bertuzzi MariaElena B. Zavala Jodi Nunnari Kandice Tanner Paulo Caceres Lee Ligon Ex Officio Greenfield Sluder Alissa M. Weaver Anupam Das Stefano Bertuzzi David Wasielewski Liaison Brooke Gardner Public Policy Ex Officio Arunika Das Alyssa Lesko Connie M. Lee, Chair Stefano Bertuzzi Ex Officio Wahid A. Mulla Charles A. Easley Shirley M. Tilghman Stefano Bertuzzi Sushama Sivakumar Daniel A. Fletcher Peter Walter Guangpu Li Cristian Suarez Lawrence S. B. Goldstein
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 9 ASCB Officers
Shirley Tilghman Peter Walter Pietro De Camilli Jennifer Lippincott- Kathleen Green Gary Gorbsky Stefano Bertuzzi President President-Elect President-Elect Schwartz Secretary Treasurer ex-officio Designate Past President
ASCB Council
Martin Chalfie Anthony Hyman Daniel Kiehart Ruth Lehmann Ian G. Macara Laura Machesky
Ira Mellman Tom Misteli Denise Montell Jodi Nunnari Samara Claire E. Walczak Reck-Peterson ASCB Committee chairs, Co-Chairs
Ted Ho Bruno Da Rocha-Azevedo Kathleen Green Gary Gorbsky Renato J. Aguilera Andrew Campbell COMPASS COMPASS Membership Finance and Audit Minorities Affairs Minorities Affairs
Simon J. Atkinson Connie Lee Sandra K. Masur Don W. Cleveland Yixian Zheng Julie Theriot Susan M. Wick Public Information Public Policy Women in Cell Biology Nominating International Program Education Affairs
CBE—LIFE SCIENCES EDUCATION MOLECULAR BIOLOGY OF THE CELL
Erin L. Dolan David G. Drubin Editor-In-Chief Editor-In-Chief
10 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting General Information REGISTRATION INFORMATION Certificates of Attendance Certificates of Attendance may be obtained at the Certificate of Scientific Registration Lobby D Attendance counter located in the Registration area next to the 619-525-6205 Attendee Services and Membership Counters.
Hours: Student Monitors/Volunteers should go the ASCB Management Saturday 8:00 am-7:00 pm Office located in Room 22 to pick up their badge. Sunday 7:30 am-6:30 pm Monday 7:30 am-6:00 pm ASCB LEARNING CENTER Tuesday 7:30 am-6:30 pm Exhibit Halls D-H Wednesday 7:30 am-11:30 am The ASCB Learning Center is the networking hub of the meeting. Exhibitor Registration Lobby D You can: 619-525-6206 • View thousands of posters, and 18 Microsymposia (ePoster Talks) highlighting the latest in basic research Thursday 1:00 pm-5:00 pm • Learn the latest technology, and how to use it, from Friday 8:00 am-5:00 pm company representatives through hands-on, in-booth Saturday 8:00 am-7:00 pm demonstrations as well as Tech Talks in dedicated Sunday-Tuesday 7:30 am-4:00 pm theaters Wednesday CLOSED • Talk to funding agencies’ program directors one-on-one • Interact directly with leaders in the field at Science Saturday Only Badge Pick-Up Counter Lobby D Discussion Tables Saturday 8:00 am-7:00 pm • Attend numerous career development programs, and take advantage of onsite CV and application materials The ASCB has a High School Program, an Undergraduate Program, review with senior scientists at the Career Center and opens its Keynote to the public on Saturday. Participants will • Network, network, network be provided stick-on white badges for pick up at the Saturday Only Badge Pickup counter. These badges allow access to the High Hours*: School Program and Undergraduate Program, Poster Competition, Saturday 5:30 pm-8:00 pm and the Keynote Symposium. Sunday-Monday 7:30 am-8:00 pm
Tuesday 7:30 am-4:00 pm Wednesday CLOSED Badges * Note: The Exhibits are open on Sunday through Tuesday from Badges are picked up onsite at the San Diego Convention Center. 9:30 am to 4:00 pm. Once you receive your badge, you will be provided with your lanyard, a meeting bag, and meeting materials. Meeting badges must be worn at all times while in the convention center. ASCB Booth Booth 721, Hall D Stop by the ASCB Booth—your one-stop shop for all things ASCB. Your badge functions as a name badge and an exhibit inquiry Browse our selection of ever-popular t-shirts and merchandise, card so exhibitors can scan your badge and then send you further view educational resources, meet some ASCB staff members, and information. learn about our organization and what we can do for you! Green badges indicate full meeting access to exhibits and sessions. Orange badges indicate meeting access to the ASCB Learning Sunday 9:30 am-4:00 pm Center (Exhibit Hall) only. Everyone over the age of 17 must have a Monday 9:30 am-4:00 pm badge. Badges will not be issued to children under the age of 17. A Tuesday 9:30 am-4:00 pm color strip will appear on badge holders of attendees with one-day Wednesday CLOSED registration. The day of registration will be printed at the bottom of the badge. ASCB Exhibit Management Hall D 619-525-6209 Attendee Guest Badges Exhibitors may visit the Exhibit Management office at the following A guest is a nonscientist family member or nonscientist friend times for assistance while at the meeting. of a registered scientist. If a registered scientist would like a family member or friend to see their invited talk or their poster Friday 9:00 am-5:00 pm presentation, the registered scientist should request this onsite at Saturday 9:00 am-5:00 pm the ASCB Meeting Management Office, Room 22, at the San Diego Sunday 9:00 am-5:00 pm Convention Center. The guest badge will only be valid for the day Monday 9:00 am-5:00 pm selected. Tuesday 9:00 am-5:00 pm Wednesday CLOSED Badge Replacement Policy You will be able to obtain a replacement badge onsite for a fee of ASCB Theater 1 (Hall G) and Theater 2 (Hall D) $15 at the Badge Replacement Counter during registration hours. Tech talks will be presented in Theater 1 and Theater 2 Sunday If you lose the second badge and need a third, you will need to through Tuesday during the meeting. Please check the daily re-register for the meeting and pay the onsite registration fee. A schedules for specific tech talk titles, presenters, dates, and times. photo ID is necessary in both cases.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 11 Housing, onPeak Official Housing Partner ASCB Booth, 721 Poster Board Assignments at ASCB Booth The ASCB has assigned all poster boards with the letter “B” followed 619-525-6203 by a three or four digit number to correspond with the aisle number and poster board number: Sunday-Tuesday 9:30 am-4:00 pm On Saturday only, the housing desk will be located in the Registration Example 1: Your poster has been assigned to “B340” area, Lobby D. B=Board; 300=Aisle in which the board is located; B340=Board Note: Have issues with your hotel? Ask onPeak for assistance. While number on which to place your poster. you’re there, make your hotel reservations for the 2016 Annual Meeting in San Francisco. Example 2: Your poster has been assigned to “B1034” B=Board; 1000=Aisle in which the board is located: B1034=Board Roundtable Central 1, 2 and 3 Halls H number on which to place your poster. Various sessions will be held at Roundtable Central sections 1, 2, and 3 including Career Discussion and Mentoring Roundtables, Science Online Poster/Abstract Viewing Discussion Tables, and various other professional development From Thursday, December 10 through Wednesday, December 16, sessions. Please check the daily schedules for specific program dates, registered meeting participants will be able to visit a password- and times. protected website to search and view abstracts, oral presentation slides, and uploaded posters. This will allow participants who miss Career Center Hall D posters they were hoping to see view the posters (or abstracts The Career Center is open whenever the ASCB Learning Center is if a poster was not uploaded), and connect with the presenter. open. Presentations and other activities will take place during the Participants will be able to contact the presenter with any questions following hours: directly through this website while viewing the poster. To search and view the abstracts/posters visit: http://www.ascb.org/amabstract. A Sunday-Tuesday 9:00 am-4:00 pm login and password will be provided to all registered attendees. Wednesday CLOSED
The Career Center offers a full lineup of networking and professional Microsymposia (ePoster Talks) Microsymposia Rooms 1 development programs. You can attend talks about careers and and 2, Hall H professional development, access job listings, and sign up each day for one-on-one career coaching and/or CV review. Microsymposia offer a new, exciting, opportunity for a short presentation at the ASCB meeting. Six Microsymposia will be Poster Presentation Information presented on Sunday through Tuesday between 12:00 pm and Poster Sessions Halls D-H 4:00 pm. Please see the daily schedules for a complete list of Posters Open for Viewing: Microsymposia. Saturday 6:00 pm-8:00 pm Sunday-Monday 7:30 am-8:00 pm Each Microsymposium session will include seven five-minute Tuesday 7:30 am-4:00 pm Microsymposia presentations. Following all seven presentations, each Wednesday CLOSED ePoster will be displayed on one of seven screens around the back of the room for 30 minutes of informal discussion. Poster Sessions: Sunday-Tuesday 12:00 pm-3:00 pm ATTENDEE AND SAN DIEGO CONVENTION Odd-Numbered Presentations: 12:00 pm-1:30 pm CENTER RESOURCES Even-Numbered Presentations: 1:30 pm-3:00 pm San Diego Convention Center Poster set up for Sunday viewing: 111 W. Harbor Drive Saturday 5:30 pm-6:00 pm San Diego, CA 92101 Poster set up for Monday viewing: Sunday 6:00 pm-6:30 pm ASCB Attendee/Membership Services Registration, Lobby D Poster set up for Tuesday viewing: 619-525-6202 Monday 6:00 pm-6:30 pm Saturday 8:00 am-7:00 pm Poster tear down on Sunday 5:30 pm-6:00 pm Sunday 7:30 am-6:30 pm Poster tear down on Monday 5:30 pm-6:00 pm Monday 7:30 am -6:00 pm Poster tear down Tuesday 3:30 pm-4:00 pm Tuesday 7:30 am-6:30 pm Wednesday 7:30 am-11:30 am * On Tuesday, December 15, posters must be removed from boards between 3:30 pm and 4:00 pm. Posters left on boards after 4:00 ASCB Meeting Management Room 22 pm on Tuesday, December 15, will not be returned to presenters. 619-525-6200 The ASCB is not responsible for posters left on the boards or poster containers left unattended. Meeting participants and poster Saturday 7:30 am-7:00 pm presenters are not allowed access to the ASCB Learning Center after Sunday-Tuesday 7:30 am-6:00 pm 4:00 pm on Tuesday, December 15 for safety reasons. No exceptions. Wednesday 7:30 am-1:00 pm
ASCB Newsroom Room 23A Friday 8:00 am-5:00 pm Saturday-Tuesday 8:00 am-6:00 pm Wednesday CLOSED 12 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting ATMs Mrs. Fields Cookies, Lobby D 8:00 am-5:00 pm The San Diego Convention Center has two onsite automatic teller Mrs. Fields Cookies, 20A Foyer 9:00 am-12:00 pm and machines (ATMs) located in Lobby B2 and Lobby E. 3:00 pm-6:00 pm
Business Center, FedEx Office Lobby D Wednesday 619-525-5450 Starbucks E, Lobby E 7:30 am-1:00 pm Auntie Anne’s Pretzel, Lobby D 8:00 am-1:00 pm Services include photocopying, office supplies, faxing, computer work Auntie Anne’s Pretzel, 20A Foyer 9:00 am-1:00 pm stations, printing, email receipt, and FedEx. Mrs. Fields Cookies, Lobby D 8:00 am-1:00 pm Mrs. Fields Cookies , 20A Foyer 9:00 am-1:00 pm Saturday-Monday 9:00 am-5:00 pm Tuesday-Wednesday 8:00 am-5:00 pm *Beer and wine will be available at these locations.
Childcare Arrangements Foreign Currency Exchange Onsite childcare services may be available through your hotel Foreign currency exchange and travelers’ insurance services are concierge. Individual or group sitters may be arranged to provide in- available daily at two locations in Terminal 2 of the San Diego room hotel childcare. Please check with your hotel well in advance of International Airport: in the Baggage Claim area (5:00 am-6:00 pm) your arrival date. and in the gate areas (5:00 am-1:00 pm, 4:30 pm-7:30 pm). There is also one foreign currency exchange in the Gaslamp district: Travelex It is the responsibility of the parent(s), guardian, legal guardian, or Currency Services, 177 Horton Plaza Level 1, 619-235-0901. individual requesting childcare services to screen caregivers and to make a determination as to the appropriateness of the caregiver. The Ground Transportation ASCB does not screen any of the childcare services and assumes no responsibility with respect to these services and accepts no liabilities. Shuttles and taxi services are a convenient way to navigate the city and are ideal for airport pick-ups and drop-offs, as well as going to and from the convention center. Coat Check/Luggage/ Poster Storage Lobby D $3 per checked coat, poster tube, and checked luggage Taxis Taxis are a convenient way to get around. The list below contains Saturday 8:00 am-10:30 pm contact information for some of the most frequently used taxi Sunday-Tuesday 7:30 am-9:00 pm companies in the city. Please call them directly with any questions or Wednesday 7:30 am-1:00 pm to request a pick up. Not all taxi companies accept credit cards. If you would like to play with a credit card, please request this when you Note: Luggage is not permitted in meeting rooms or in the Learning call. Center (Exhibit Hall). Airport Yellow Cab of San Diego 619-444-4444 First Aid Lobby E, Show Box Office E American Cab 619-234-1111 619-525-6212 Orange Cab 619-291-3333 San Diego Cab 619-226-8294 / 800-368-2947 Saturday 7:30 am-10:30 pm USA Cab 619-231-1144 Sunday-Tuesday 7:30 am-9:00 pm Wednesday 7:30 am-1:00 pm SuperShuttle On Tuesday from 9:00 am-6:00 pm and Wednesday 8:00 am -1:00 In a life-threatening emergency, use a house phone (located pm, SuperShuttle will take reservations from attendees who wish to throughout the center) and call 5911. From an outside mobile phone take a shuttle to the airport. Their table will be located next to the dial 619-525-5911. Do NOT call 911 directly. Coat/Poster/Luggage Check in the Registration Area in Lobby D.
Housing, onPeak Official Housing Partner Lobby D Food and Beverage in the Convention Center The following concessions will be open in the Convention Center Saturday 8:00 am-6:00 pm (hours are subject to change and stands are subject to closure based on traffic): The housing desk will move to the ASCB Booth, 721, in the ASCB Learning Center (Exhibit Hall) on Sunday at 9:30 am. Saturday Starbucks E, Lobby E 7:30 am-5:00 pm Housing, onPeak Official Housing Partner ASCB Booth, 721 Auntie Anne’s Pretzel, Lobby D 8:00 am-5:00 pm 619-525-6203 Auntie Anne’s Pretzel, 20A Foyer 9:00 am-6:00 pm Sunday-Tuesday 9:30 am-4:00 pm Mrs. Fields Cookies, 20A Foyer 9:00 am-6:00 pm Mrs. Fields Cookies, Lobby D 8:00 am-5:00 pm On Saturday, the housing desk will be located in the Registration area, Lobby D. Sunday-Tuesday Starbucks C, Lobby C 7:30 am-3:00 pm Note: Have issues with your hotel? Ask onPeak for assistance. While Starbucks E, Lobby E 7:00 am-5:00 pm you’re there, make your hotel reservations for the 2016 Annual Café Express*, Hall E 11:00 am-2:30 pm Meeting in San Francisco. Café Express*, Hall F 11:30 am-3:00 pm Auntie Anne’s Pretzel, Lobby D 8:00 am-5:00 pm Infant Lounge Auntie Anne’s Pretzel, 20A Foyer 9:00 am-12:00 pm and A nicely furnished permanent Infant Lounge is located in the 3:00 pm-6:00 pm Women’s restroom behind Starbucks in the Hall E Lobby of the San
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 13 Diego Convention Center. Look for a sign with arrows pointing to it Lost and Found ASCB Meeting Management Office, Room 22 just outside the Starbucks. The room has tables and chairs, electricity, 619-525-6200 and water. The semi-private lounge, available for nursing mothers and parents with infants, provides a comfortable and secure environment. Items turned in are kept throughout the meeting until Wednesday, Parents and guardians are responsible for providing infant care December 16 at 12:00 pm. After this time, items will be turned over supplies. The infant lounge is unsupervised, and the ASCB is not to the San Diego Convention Center, where they are kept for 60 days responsible for any accidents or injuries that may occur. The infant from the close of the meeting. lounge is open at all times the San Diego Convention Center is open. Message Center Lobby D Internet Access, Wireless A message center will be available during the meeting for you to Complimentary wireless Internet access will only be available in leave messages for other attendees. On Wednesday, December 16, at Lobby D-H (lower lobby at the Convention Center. For complimentary 1:00 pm, the Message Center will be dismantled, and any messages access Set the SSID (wireless network identifier) to Free Internet. left on the boards will be discarded.
Complimentary access is not available in the ASCB Learning Center Mobile App (Exhibit Hall), on the upper level lobby, or in any meeting rooms Visit the Google Play or Apple Store to download the 2015 ASCB (except the Large Scale Data Workshops). Paid access is available in Meeting App. Search ''ASCB15'' to locate the app. these areas. Non-Denominational Prayer Room Room 18 Attendee paid access to Internet in the upper lobby areas and Open to registered meeting participants and exhibitors looking for a meeting rooms is $12.95 per day, per device. quiet place to meditate or pray.
Exhibitor paid access to Internet in the Exhibit Hall is $79.99 per day, Saturday-Tuesday 7:30 am-7:30 pm or $159.99 for 3 days. Wednesday 7:30 am-1:00 pm
To connect to WiFi: Parking • Open your browser (Internet Explorer, Firefox, Safari, or Onsite private vehicle parking is available at the San Diego other standard browser). You should see a page resembling Convention Center. Enter the parking garage on Harbor Drive the graphic shown*. between First Ave. and Fifth Ave. The daily rate is $15. Payment is • If this is your initial purchase, enter your username (email due upon entry. No in and out privileges or overnight parking are address) and password in the area shown on the left and permitted. Call ACE Parking at 619-237-0399 with any questions. click BUY NOW. Follow additional prompts to complete your purchase or login. Refer to service options and limitations Off-site parking is also available at numerous nearby parking lots and shown to the right. garages in downtown San Diego; many are within walking distance • If you have already created an account and are returning of the center. Lots and garages are individually owned and operated, for an additional session, click LOGIN. prices vary by location.
Metered street parking is available in some areas. Parking meters are enforced Monday through Saturday, from 8:00 am until 6:00 pm, unless otherwise posted. Metered spots are free on Sunday and designated holidays.
Poster Printing Service Pick-Up Registration Counter, Lobby D (Makesigns.com)
If you ordered your poster through our official poster printing company, Makesigns.com, then you can pick it up during the *If you do not see the above screenshot when you open your following hours: browser, please refresh your browser. If you still do not see this Saturday, Dec. 12 8:00 am-7:00 pm page you may need to manually select the SSID (network name) Sunday, Dec. 13 7:30 am-6:30 pm by following your computer’s procedure for viewing and selecting Monday, Dec. 14 7:30 am-6:00 pm a wireless network. The Smart City SSIDs to look for are “Exhibitor Tuesday, Dec. 15 7:30 am-1:00 pm Internet” or “Instant Internet.” Restaurant/Concierge Lobby E Meeting attendees who wish to use the service should bring their 619-525-5610 own laptop computer or PDA, with a wireless 802.11b/g network card installed. Onsite technical support will not be provided. ASCB has partnered with the San Diego Convention Center to provide a complimentary concierge service for meeting participants Please configure your wireless connection before coming to the and exhibitors. The Convention Center staff will be happy to make meeting. Remember to consider the security implications of using the restaurant recommendations and reservations as well as provide wireless network and protect your laptop accordingly. information about shopping and local sightseeing.
Internet Café Registration, Lobby D Saturday 10:00 am-6:00 pm The Internet Café will provide attendees with access to email and Sunday-Tuesday 9:00 am-6:00 pm the web. The Internet Café is accessible during registration hours. Participants are asked to limit their use to 10 minutes per session when others are waiting.
14 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Sales Tax There are two TTY phones in the Convention Center: The sales tax in San Diego is 8%; city hotel tax is 12.5%; food and • Admin Receptionist in the Administrative Office area, located on beverage tax is 8%. mezzanine level by taking elevator in lobby E or upper level by room 21, to the mezzanine and exiting to the right Shuttle Bus Service • Lobby 6, bayside TTY is connected to one of the three remaining ASCB does not provide shuttle service for this meeting. See "Ground payphones. Transportation" for more information on how to get around. If you require special services, and did not mark the appropriate box during the Meeting Registration process, please visit Room 22, ASCB Tipping Meeting Management, for assistance. Tipping is standard. Here are a few tipping guidelines: wait staff, 15- 20%; taxi drivers, 15%; doormen, skycaps, and porters, $2 per bag; hotel Scooter and Wheelchair Rental maids, $2-$3 per room, per night. Call 888-441-7575 to contact Scootaround, or visit http://locations. scootaround.com/rentals/s/sandiego/events.htm. For additional Weather rental companies, view the San Diego Convention Center website: San Diego temperatures in December may vary, but are typically chilly. http://www.ascb.org/2015meeting/accessibilityada/. You should bring layers of clothing and be sure to have a sweater, jacket, or coat as well as an umbrella. Temperatures in December average a LSA Interpretation Services high of 66°F/18°C to a low of 49°F/9°C. December average rainfall is 1.3 If you need translation services for the 2015 ASCB Annual Meeting, inches/3.3 cm. contact Language Services Associates (LSA) directly at 800-305-9673 or www.LSAweb.com. To get a more timely sense of the weather, visit http://www.weather. Language Services Associates is a nationwide full-service firm com. You will find information about current conditions, as well as a five- providing translators and interpreters in 180 languages. day forecast.
What to Wear MEETING POLICIES Wear comfortable shoes—you’ll be doing a lot of walking. Most attendees dress in jeans and casual wear. Bring a jacket or sweater Camera, Cell Phone, Recording, Photography, and Session Etiquette because it can get cold in some rooms in the convention center. While you are in a session, please mute all cell phones and other electronic devices. If necessary, please step out to the hallway to SPEAKER RESOURCES make a call, or send an email or text. The back-lighting on electronic devices is distracting in a dimmed room. The Society strictly prohibits Speaker Lounge Room 26AB photography and all electronic capture or audio recording of scientific 619-525-6210 sessions in meeting rooms or the Learning Center (Exhibit Hall). This policy also includes photographing colleagues against the backdrop Saturday 8:00 am-6:00 pm of scientific posters on display without the express consent of the Sunday-Tuesday 7:00 am-6:30 pm presenting author(s). Wednesday 7:30 am-11:00 am These policies will be enforced by the Society. Individuals who do not All invited speakers are asked to stop by the Speaker Lounge well in comply will be asked to leave the session or Learning Center (Exhibit advance of their scheduled presentation, to allow time to review, Hall) floor and not be allowed room re-entry. Repeat offenders will rehearse, upload their presentation to the system to be sent have their meeting badge(s) revoked and will not be allowed to electronically from Speaker Lounge to the session room, and take care of continue to attend the meeting. This policy is necessary to respect any presentation concerns. the willingness of presenters to share their data at the meeting as well as their publication opportunities. If you have any questions Speaker Briefings for Symposia and Minisymposia Chairs and Speakers regarding these policies, please contact Alison Harris in Room 22. Speaker briefings in the Speaker Lounge are held for Symposia and Minisymposia speakers and chairs only. There are no speaker briefings Children for Subgroup or Microsymposia speakers. Speaker briefings are held Children of ASCB meeting participants are welcome to attend the Saturday through Tuesday from 3:00 pm to 3:30 pm. Symposia speakers Annual Meeting with their parent or guardian as long as the child is and Wednesday Minisymposia speakers should attend the briefing the under the supervision by a parent or guardian at all times, and they day before their session. All Sunday, Monday, and Tuesday Minisymposia do not disrupt the event. speakers and chairs should attend the speaker briefing on the day of their session and then head from the speaker briefing to the session Strollers are allowed in the meeting rooms and the Learning Center room. If you are unable to attend your assigned speaker briefing, we (Exhibit Hall) during the meeting hours. Please be aware that small recommend you at least stop by the Speaker Lounge to check your children in strollers are at the same level as someone carrying a presentation well in advance of your session. poster tube, bag, briefcase, umbrella, or other object.
Under no circumstances are children under the age of 17 allowed in Accessibility for People with Disabilities the Learning Center (Exhibit Hall) during set-up and dismantle times. and Special Needs This includes children of exhibitors.
The San Diego Convention Center is ADA compliant. In accordance Drinking Policy with the ADA, the Center is responsible for permanent premises access The ASCB and the San Diego Convention Center encourage accommodations, such as wheelchair lifts, elevator standards, door width responsible drinking for those drinking alcohol. Beer, wine, non- standards and restroom accessibility. The Center’s underground parking alcoholic beer, and soft drinks will be offered at the Opening Night garage also has 31 ADA compliant parking stalls and elevator access. Reception on Saturday, December 12, and throughout the meeting
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 15 dates. Alcohol will not be served to anyone under the age of 21; Social Media Policy be prepared to show photo identification. Alcoholic beverages are The ASCB encourages the use of social media before, during, allowed only in specific areas and must not be taken out of these and after the 2015 Annual Meeting. Please note the following areas. guidelines and accepted social media etiquette:
Photo Release Do The ASCB has hired an official photographer for the meeting. • Follow us on Twitter @ASCBiology. Use the hash tag Photographs taken at the 2015 ASCB Meeting may be used in future #ASCB15 for Annual Meeting-related tweets. ASCB publications, on the ASCB website, or in other Society materials. • Follow us on Facebook at facebook.com/ASCBiology. By registering for this meeting, you agreed to allow the ASCB to use • Blog and tweet about the meeting and what you are your photo in any ASCB-related publications or on the website. hearing and seeing (but without sharing details of any data presented; follow journal rules about data sharing). Session Room Behavior • Provide feedback to ASCB staff and the Program If a session room becomes too crowded, the ASCB asks that you Committee—discuss topics of interest and/or speakers follow instructions provided by ASCB staff, convention center for next year’s meeting, make suggestions for Symposia, staff, or security. Instructions may include not standing against the Minisymposia, translational or workshop sessions, walls, not blocking the aisles or doors, or being denied entry if the comment on the meeting format, etc. room becomes too crowded. The ASCB is obligated to abide by the • Keep criticism constructive, and think about whether you’d guidelines established by the Fire Marshal. If a room reaches full like your thoughts shared widely—because they may be. capacity and we do not have your cooperation, the Fire Marshal has the authority to delay or terminate the meeting until the problems Don’t have been corrected. • Use photographic or other recording devices—these are prohibited in scientific sessions, poster sessions, and in the Smoking Policy Learning Center (Exhibit Hall). The San Diego Convention Center is a smoke-free facility. Please • Capture, transmit, or redistribute data presented at the refrain from smoking within 25 feet of the lobby doors. Smoking is meeting—this may preclude subsequent publication of the permitted only in designated smoking areas. Electronic cigarettes are data in a scholarly journal. not permitted within the facility. Speaker Disclosure Views expressed by speakers at the 2015 ASCB Meeting are solely the views of the speaker. They do not necessarily represent the views of the ASCB. The ASCB makes no representation concerning, and does not guarantee, the source, originality, accuracy, completeness, or reliability of any statement, information, data, finding, interpretation, advice, opinion, or view presented by any speaker or poster presenter.
Waiver of Liability Each individual attending the 2015 ASCB Meeting assumes all risks associated with his or her attendance and participation in on- and offsite activities. Each individual participant agrees to indemnify and hold harmless the ASCB and its governing bodies, officers, directors, employees, and/or agents from all loss, damage, or liability arising out of or related to his or her attendance at the 2015 ASCB Meeting.
16 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting HOTEL INFORMATION
If you are having issues with your hotel, contact our official housing partner, onPeak, for assistance.
Housing, onPeak Official Housing Partner Registration, Lobby D Saturday 8:00 am-6:00 pm
Housing, onPeak Official Housing Partner ASCB Booth 721, Learning Center Sunday-Tuesday 9:30 am-4:00 pm or call 619-525-6203 during hours listed above.
SAFETY TIPS AND EMERGENCIES Safety Tips The safety and security of participants and staff is the ASCB’s first Walk “smart” when you leave the convention center: priority. We are committed to make every effort to ensure a safe and • Know your destination and the best way to reach it. productive event for everyone. We ask our participants to be aware • Take off your name badge when exiting the building. of their surroundings at all times and note the closest emergency exit • Travel along sidewalks in lighted areas at night, and don’t walk in all facilities. alone. • Establish a “buddy” system with another participant. Non Life-Threatening Emergency: Use a house phone (located • Share schedules and check up on each other periodically. throughout the center) to dial Security/Guest Services at extension • Build your awareness of unknown surroundings by reviewing 5490 or from an outside line or mobile phone, call 619-525-5490. local information. • Secure your laptop computer, which is an attractive, easy target Life Threatening Emergency: Use a house phone to dial extension for thieves. 5911; or from an outside line or mobile phone, call 619-525-5911. • Women can wear jackets with pockets instead of carrying a handbag that might get lost or stolen. For emergencies while in your hotel, please follow the hotel’s specific instructions. The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 17 Convention Center maps
Upper level
Mezzanine level
lower/exhibit level
18 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Convention Center maps
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 19 ASCB 2015 Travel AWARD recipients 2015 Education Committee Travel Awards
Travel awards for the following faculty at primarily teaching Cathy Ramos, National Institutes of Health institutions were made possible by generous support from Stefanie Redemann, TU Dresden/MTZ the Company of Biologists: Carlos Reis, University of Texas Southwestern Medical Center Kate Cooper, Loras College Svetlana Rubtsova, Russian Cancer Research Center Nathan Derr, Smith College Etiene Sauvageau, INRS-Institut Armand-Frappier Scott Gehler, Augustana College Shih-Chieh Ti, The Rockefeller University Jennifer Hurst-Kennedy, Georgia Gwinnett College Lalitha Jayant, Borough Manhattan Community College Meeting registrations for the following postdoctoral fellows Lani Keller, Quinnipiac University were made possible by generous support from Sanofi: Gavin McStay, New York Institute of Technology Ahmad Athamneh, Purdue University Jay Pieczynski, Rollins College Alexander Blasky, University of Colorado Anschutz Medical Megan Povelones, Penn State Brandywine Campus Karen Resendes, Westminster College Andrew Holle, Max Planck Institute for Intelligent Systems Cindy Voisine, Northeastern Illinois University Corrine Kliment, Johns Hopkins University Medical Institute Mark Wallert, Bemidji State University Caleb Trujillo, ichigan State University Bin Wei, University of Tennessee Travel awards for the following faculty at primarily teaching institutions were made possible by generous support from Travel awards for the following graduate students were made the ASCB: possible by Chroma Technology Corporation: Ranan Aktas, Maltepe University Rebecca Adikes, Univ North Carolina Fabio Forti, University of Sao Paulo, Brazil Jessica Cole, Rollins College & Portland State University Diana Jaalouk, American University of Beirut Lauren Del Bel, University of Toronto Anne Powell, University of Oregon Kevin Kruse, University of Illinois at Chicago Brian Sato, University of California, Irvine Joseph Siefert, Oklahoma Medical Research Foundation Lubna Tahtamouni, Hashemite University Richard Smindak, University of Virginia Pavan Vedula, Rutgers University Travel awards for the following postdoctoral fellows were Laura Zahn, Austin Peay State University made possible by generous support from the Company of Biologists: Travel awards for the following graduate students were made McKale Davis, Midwestern University possible by the ASCB: Gal Haimovich, Weizmann Institute of Science Max Adrian, Utrecht University Zhong Liu, University of Alabama Birmingham Zahra Alvandi, University of Torornto Joshua Morgan, University of Delaware Souparno Bhattacharya, University of Texas Southwestern Patrycja Pietruszka, Université du Québec à Chicoutimi Medical Center Rashmi Priya, University of Queensland Andrew Boyce, University of Victoria M Angeles Rabadan, Memorial Sloan Kettering Cancer Center Lauren Burianek, Duke University Jaakko Sarparanta, Albert Einstein College of Medicine Salvatore Calise, University of Florida Maria Sckolnick, University of Vermont Chi-Han Chang, Harvard University Arati Tripathi, Harvard Medical School Mahesh Chemudupati, The Ohio State University Tatsuhisa Tsuboi, University of California, Irvine Wei-Sheng Chen, Tufts School of Medicine Katherine Vest, Emory University Nathan Cox, Duke University Valerie Vilmont, Myology Research Center Jonathan D’Amico, University of Notre Dame Frederick Westhorpe, Stanford University Matthew Dean, Louisiana State University Health Sciences Center Travel awards for the following postdoctoral fellows were Wei Yung Ding, National University of Singapore made possible by generous support from the ASCB: Kelsie Eichel, University of California, San Francisco Xiaojing An, University of Massachusetts Amherst Kristen Felt, Bucknell University Sara Baratchi, RMIT University Natalya Gertsik, Weill Cornell Medical College Sangyoon Han, University of Texas Southwestern Medical Siyang Guo, Brandeis University Center Katharina Hennig, University Joseph Fourier Afm Islam, University of Arizona Wan-Ying Hsieh, Memorial Sloan Kettering Cancer Center Iman Jalilian, University of New South Wales Timothy Jackson, University of Pittsburgh Dae Joong Kim, University of North Carolina at Chapel Hill Xue Jiang, Texas A&M University Karthik Krishnan, University of Cincinnati Menuka Karki, New Mexico State University Nir Qvit, Stanford University Seda Kilinc Avsaroglu, Wesleyan University
20 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Anna Kobb, University of Toronto Alice Chen-Liaw, University of Scranton Anuttama Kulkarni, Tata Institute of Fundamental Research Samantha Chin, Brandeis University Dhivya Kumar, University of Connecticut Health Center Margaret Magaletta, Rollins College Nicholas Lim, University of Melbourne Rose Massey, Austin College Monika Magon, University College London Thomas Meister, Duke University Pranav Mathur, University of Utah Aylin Memili, Mississippi School for Mathematics and Science Tyler Moyer, Johns Hopkins Medical Institute Alexander Merriman, Duke University Tin Ngo, University of California, Davis Meredith Mishler, Westminster College Megan Novak, Northwestern University Kathleen Nevola, Stevens Institute of Technology Sarah Ohlen, Montana State University Sahba Seddighi, University of Tennessee Luke Oostdyk, University of Virginia Karl Petersen, University of Minnesota Brittany Phillips, Emory University 2015 childcare Awards Manmeet Raval, Penn State College of Medicine Rini Ravindran, Louisiana State University Health Sciences The ASCB Women in Cell Biology (WICB) Committee has Center selected the following individuals to receive childcare grant Abhirup Sarkar, Rowan University travel awards, which are funded through a generous grant Maggie Scavello, University of Arizona from Nature Publishing Group: Melike Sever, Bilkent University UNAM Sara Baratchi, RMIT University Nadezda Stepicheva, University of Delaware Topher Carroll, Yale University Claire Stewart, Colorado State University Maitreyi Das, University of Tennessee, Knoxville Emily Summerbell, Emory University Seda Kilinc Avsaroglu, Wesleyan University Nilay Taneja, Vanderbilt University Corrine Kliment, Johns Hopkins University Medical Institute Vivian Tang, University of Western Australia Laura Lowery, Boston College Gregory Thompson, South Dakota State University Teresa Luperchio, Johns Hopkins School of Medicine Kathryn VanDenHeuvel, University of Wisconsin Madison Patrick Lusk, Yale University School of Medicine Aditya Venkatesh, University of Massachusetts Medical School Stephen Moore, Northeastern University Jordan VerPlank, Hunter James Kelly Research Institute Kassandra Ori-McKenney, University of California, San Nicholas Wohlgemuth, Johns Hopkins University School of Francisco Public Health Sabine Petry, Princeton University Jiaqi Xu, Zhejiang University School of Medicine Stefanie Redemann, TU Dresden/MTZ Zijian Xu, National Institute of Biological Sciences, Beijing Daiane Santana Alves, University of Tennessee Raj Yadav, Chonbuk National University Maria Sckolnick, University of Vermont Yao Yan, Emporia State University Beth Weaver, Univerisity of Wisconsin, Madison Huijuan Yuan, New York Medical College Cecilia Zurita-Lopez, California State University, Los Angeles Ana Carvalho, Institute of Molecular and Cellular Biology Travel awards for the following undergraduate students were Florencia Del Viso, Yale University made possible by Worthington Biochemical Corporation: Nathan Derr, Smith College A. Karim Embong, Rochester Institute of Technology Amel El Bahloul, CNRS/Institut Pasteur Arya Dahal, College of William and Mary Luciana Gallo, University of Pittsburgh Da In Lee, Northeastern Illinois University Abigail Gerhold, University of Montreal Eliot Blatt, Rhodes College Allyson O’Donnell, Duquesne University Erin Rutherford, Boston College Sara Olson, Pomona College Matthew Reynolds, University of Scranton Hannah Picariello, Cleveland Clinic Melissa Bischak, Westminster College Eva Schmid, University of California, Berkeley Minhkhoi Nguyen, University of Arizona April Scruggs, University of South Alabama Nicole McAndrew, University of Scranton Julie Yi, Columbia University
Travel awards for the following undergraduate students were made possible by the ASCB: Carolyn Ahlers, University of Notre Dame Christopher Alderman, Emporia State University Joel Barrett, Jr., Austin College Victoria Burgess, Georgia Gwinnett College
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 21 2015 minorities affairs Committee Chris Suarez, University of Notre Dame Travel Awards David Thoms, Indiana University Fabio Urbina, University of North Carolina: Chapel Hill The ASCB Minorities Affairs Committee has selected the Lauren Walker, University of California, Riverside following students and scientists to receive travel awards, Scott Wilkinson, Emory University which are supported by a Howard Hughes Medical Institute Susana Wilson-Hawken, Williams College grant supporting the Visiting Professors Program and a generous contribution by the Burroughs Wellcome Fund. Cindy Achat-Mendes, Georgia Gwinnett College 2015 INTERNATIONAL TRAVEL AWARDS Andrea Ambrosio, Colorado State University Travel awards for the following were made possible by Itzel Armendariz, University of Texas of the Permian Basin generous support from the ASCB: John Beckford, University of Massachusetts Amherst Danielle Beckman, Federal University of Rio de Janeiro Cheryl Bell, University of Pittsburgh Umar M. Bello, University of Pretoria Shyretha Brown, Tennessee State University Pratibha Chanana, Jawahar Lal Nehru University, Delhi Patrice Cagle, North Carolina A&T State University Alicia Chi, University of Cape Town Andrea Cantu, University of California, San Francisco Srishti Dar, Indian Institute of Science Education and Research Irene Cofie, Dartmouth University Dima Diab El Harakeh, American University of Beirut Lissette Cruz, Rutgers University Alaide Domínguez-Calderón, Cinvestav Blanca Diaz-Rohrer, University of Texas Health Science Center Farah Haque, National Centre for Biological Sciences Houston Lilian Hill, Universidade Federal do Rio de Janeiro Santiago Di Pietro, Colorado State University Sunny Kataria, Institute for Stem Cell Biology and Regenerative Devon Ehnes, University of California Riverside Medicine Andrea Ellis, New Mexico State University Kamalesh Kumari, National Centre for Biological Sciences Andrea Estrada, Colorado State University Daniela Ogias, University of São Paulo Shanice Faison, Prairie View A&M Pilar Pedro, Universidad Nacional de Cordoba Yismeilin Feliz-Mosquea, Inter American University of Puerto Rico Ajeena Ramanujan, Jawaharal Nehru University Donna Gilbert, Prairie View A&M University Arturo Raya-Sandino, Center for Research and Advanced Monica Gireud, University of Texas Health Science Center - Studies (Cinvestav) Houston GSBS Fernanda Roversi, University of Campinas Megan Gnazzo, University Wisconsin-Madison Mugdha Sathe, National Center for Biological Sciences Kim Gonzalez, Rice University Daniel Serrano, University of Sherbrooke David Iaea, Weill Cornell Med College Divya Singh, Banaras Hindu University Nicole Jackson, University of Louisville Hind Zahr, American University of Beirut Robert Kao, Seattle Children’s Research Institute/University of Washington Travel awards for the following were made possible by Bwarenaba Kautu, Greenville College generous support from the International Centre for Genetic Paul Kim, Grambling State University Engineering and Biotechnology: Anel Lizcano, University of California, San Diego Francesca Burgos, Universidad de Chile Damaris Lorenzo, Duke University Medical Center Tommaso Cavazza, Center for Genomic Regulation - CRG Anthony Mangan, University Colorado-Denver AMC Anupam Das, Albany Medical College Melvenia Martin, Grambling State University Provas Das, Indian Association for the Cultivation of Science Patrick Martin, North Carolina A&T State University Divya Ganapathi Sankaran, Anschutz Medical Campus, Christopher Medina, University of New Mexico Health Science University of Colorado Center Viraj Ichhaporia, St. Jude Children’s Research Hospital Kevin Morris, Emory University Ezgi Kunttas-Tatli, California Institute of Technology Hilmarie Muniz-Talavera, University of Illinois-Chicago Chenshu Liu, Columbia University Veronica Nagle, The College of William and Mary Masfique Mehedi, National Institutes of Health Fran Norflus, Clayton State University Sree Deepthi Muthukrishnan, University of Maine Akilah Plair, UConn Health Francesca Prendes, Cedar Crest College Travel awards for the following were made possible by Blake Riggs, San Francisco State University generous support from the immigration law firm Getson & Peter Rodriguez, Barry University Schatz, P.C.: Crystal Rogers, Caltech Vincent Boudreau, University of North Carolina at Chapel Hill Glenn Simmons, UT Southwestern Florencia Del Viso, Yale University Natalie Sirisaengtaksin, University of Texas Health Science Hasini Jayatilaka, Johns Hopkins University Center-Houston Aiko Otsubo, University of Pennsylvania Mesia Steed, Winston Salem State University Cayla Steemer, Prairie View A&M University
22 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting TRANSLATE Promise TO Progress
The ISSCR Annual Meeting is your time to connect and collaborate with scientists from around the world.
“The ISSCR Annual Meeting has a huge impact, as the major conference in the stem cell field. It is like the sum of the very best work done in the field plus a spyglass with all the trends to be fulfilled in a nearby future.”
SAVE 2015 Meeting Attendee THE DATE Engage with cutting-edge science and technology in San Francisco — Join us for the Global Stem Cell Event at ISSCR 2016. co-sponsored by: www.isscr2016.org
Booth #501
Extend Your Image Analysis Image Courtesy: Elizabeth McNeill and David Van Vactor To A New Dimension Harvard Medical School
SVCell helps you go from image to results quickly and easily. The powerful Try our recipes for your application: Analysis Cube lets you view, segment and measure objects in 3D space with • Cell Proliferation • Wound Healing unprecedented versatility. • Colony Analysis • Cell Count • Cell Tracking • Neuronal Spine Analysis With SVCell software, you load your image, pick your recipe and apply. • Neurite Tracking • Calcium Oscillation Getting results is this easy! • Nuclei Count • Nuclei Tracking • Particle Tracking • Exocytosis Event Detection
RECIPE-DRIVEN RESULTS www.svcell.com
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 23 Notes
24 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting saturday–Wednesday Schedules
ASCB /ascbiology @ascbiology ascb.org Ensure Congress and the world hears CongressEnsure and the world on hot button science ASCB works issues policy calls ASCB’s Report on Reproducibility to practices research in best for reform and the reliability ensure of published work reproducibility the latest on science policy issues the latest on science policy Opportunities to gain valuable working while leadership experience the around with other scientists from world Substantial discounts and travel Substantial discounts and travel in more (members save awards fees than ASCB registration membership actually costs) find us: us: find
Advocacy—Find your voice your Advocacy—Find Attend the premier conference conference the premier Attend on cell biology and biomedical at significant savings research
global scientific events global scientific of interest Year-round opportunities for visibility Year-round awards, and leadership including travel speaking opportunities at local and Online access to ASCB’s 9,000 members and their scientific areas worldwide CV review by senior scientists by CV review meeting at your a local Organize institution with ASCB funding Publish for free in CBE—Life SciencesPublish for free Training and leadership opportunities Training writing including grant for and recognition Cash awards student research in graduate excellence for not-yet- and recognition Cash awards independent investigators tenured in MBoC 20% discount on page charges Career coaching and career workshops development and job openings at CV for free your Post a discounted rate
The American SocietyThe American for Cell (ASCB) is an inclusive, Biology biology and biomedicalcommunity of scientists in cell international discovery, scientific dedicated to advancing are We research. education, promoting improving policies, research sound advocating in the scientific diversity and increasing development, professional workforce.
Accelerate your career your Accelerate Gain recognition, make connections, make Gain recognition, science and publish your Expand your knowledge Expand your Education (LSE) saturday–Wednesday Schedules Saturday December 12, 2015
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 25 8:00 am-7:00 pm Registration Open Registration Area
9:00-10:15 am Mentoring Keynote Ballroom 6A
10:30 am-2:30 pm Grant Writing Seminar Room 5B
1:00-5:00 pm Special Interest Subgroups A. Autophagy in Disease and Survival Room 16A B. Building the Cell Room 31B C. Cellular and Molecular Mechanobiology: New Approaches, Systems, and Responses Room 33A D: Connexins and Pannexins in Disease Room 16B E: Cytoskeletal and Membrane Protein Dynamics at the T Cell Immunological Synapse Room 23B F: Diverse Roles of Glycans and Glycan-Binding Proteins in Human Diseases Room 17A G: Dynamic Interplay between Lipids, Curvatures, and Diseases of Biological Membranes Room 28D H: Extracellular Vesicles - Biogenesis and Function Room 29B I: Increasing Diversity in a Changing Research Landscape Room 17B J: Microtubule Networks in Differentiated Cells Room 32B K: Neuronal Cytoskeleton: Cytoarchitecture and Dynamics Room 33B L: Nuclear Envelope Dynamics Room 28B M: Nucleation Phenomena in Cell Biology Room 29C N: Polymerizing Enzymes: New Frontiers in Protein Compartmentalization Room 30B and Localization O: Quantitative Microscopy and Image Analysis: Measuring Cellular Room 30D Organization and Dynamics
2:15-3:15 pm Undergraduate Program Ballroom 6A
3:30-5:30 pm Poster Competition and Reception Ballroom 6A
4:00-5:30 pm High School Program: Exploring the Microscopic World with Your Own Foldscope Room 5B
5:00-5:45 pm Meet and Greet Room 24A-C Daily Schedule—Saturday, December 12 6:00 pm Keynote Symposium Ballroom 20BC Sallie W. Chisholm, Massachusetts Institute of Technology The Honorable, Professor Jane Lubchenco, Oregon State University
6:00-8:00 pm Posters on Display Learning Center
Immediately following Opening Night Reception Sails Pavilion Keynote-10:00 pm
8:00-9:00 pm International Research and Training Exchange Fair Sails Pavilion
26 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Saturday, December 12 SATURDAY l Mentoring Keynote 9:00-10:15 am Ballroom 6A
Supported by a grant from The Burroughs Wellcome Fund SATURDAY
JoAnn Trejo Professor, Director, San Diego IRACDA, Vice Chair for Education, Department of Pharmacology, University of California, San Diego
This talk will focus on diversity in biomedical research and professional development.
l Grant Writing Seminar 10:30-2:30 pm Room 5B
Stephen W. Russell Grant Writers’ Seminars & Workshops, LLC
Geared toward senior postdocs and junior faculty, this seminar will address both practical and conceptual aspects that are important to the proposal-writing process. The focus is primarily on grant applications to the U.S. National Institutes of Health. Participants will be taught to write with a linear progression of logic, which leads reviewers through their applications. Audience questions and participation are encouraged. Preregistration was required for this program and the cost is $50. If you would like to attend, please come to Room 5B just before the start of the session to see if there is room available.
l Special Interest Subgroups 1:00-5:00 pm
The following member-organized sessions were selected by the ASCB Program Committee. All meeting attendees are welcome to participate. Meeting registration is required for both speakers and attendees.
Subgroup A: Autophagy in Disease and Survival Organizers: Nihal Altan-Bonnet, National Heart, Lung, and Blood Institute, NIH; and Rosa Puertollano, Room 16A National Heart, Lung, and Blood Institute, NIH
This subgroup will bring together a diverse group of scientists to present their latest studies on the role of cellular autophagy membrane systems in disease and survival. The autophagosomal membrane systems are critical for maintaining protein and lipid homeostasis and overall organelle and cell identity. Cutting edge basic and translational research will be presented on the mechanisms by which the autophagosomal membrane systems and associated machinery can maintain cell survival during periods of cell stress; can be subverted during oncogenesis; can be hijacked by pathogens; and can lead to devastating cardiovascular and neurodegenerative disorders when disrupted. The goal of the subgroup will be to identify key and potentially common roles played by autophagosomal membrane systems in diverse and complex diseases and to discuss the feasibility of developing therapeutic approaches targeting these pathways.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 27 Presentations: 1:00–1:05 pm Introduction 1:05–1:30 pm Intercellular transmission of viral populations by secreted autophagosomes. Nihal Altan-Bonnet, National Heart, Lung, and Blood Institute, NIH 1:30–2:00 pm Unexpected functions of autophagy during pathogenesis. Ken Cadwell, New York University 2:00–2:30 pm Harnessing the autophagy-lysosome machinery in cardiac myocytes for disease prevention and therapy. Abhinaw Dinaw, Washington University School of Medicine 2:30–3:00 pm Cell death induced by nutrient deprivation. Michael Overholtzer, Memorial Sloan-Kettering Cancer Center 3:00–3:15 pm Break 3:15–3:45 pm Autophagy and Aging: Lessons from C. elegans. Malene Hansen, Sanford-Burnham Medical Research Institute 3:45–4:15 pm Role of Autophagy in Cellular Adaptation to Stress. Rosa Puertollano, National Heart, Lung, and Blood Institute, NIH 4:15–4:45 pm Mechanisms supporting lysosomal responses to intracellular nutrient availability. Shawn Ferguson, Yale University 4:45–5:00 pm Discussion, General Questions
Subgroup B: Building the Cell Organizer: Susanne Rafelski, University of California, Irvine, and Allen Institute for Cell Science, Seattle Room 31B
Modern cell biology has made great strides in understanding cell structure and function. As with any engineering problem, however, there is a third important aspect that needs to be understood besides structure and function, and that is assembly. How are the complex three-dimensional structures found within the cell specified by a one-dimensional genome? In this session we will explore the mechanisms by which cellular structures are determined and regulated. Because this question lies at the interface of biology and physics, this Building the Cell session will be highly interdisciplinary with speakers whose interests range from physics and mathematical modeling to biochemistry and cell biology.
Presentations: 1:00–1:05 pm Introduction 1:05–1:25 pm Probing cellular biophysics with genetically encoded nanoparticles. Liam Holt, University of California, Berkeley 1:25–1:45 pm Organizing the bacterial cell with protein gradients. Anthony Vecchiarelli, National Institutes of Health 1:45–2:05 pm Organization of spindle microtubule architecture by minus-end directed kinesin motors. Melissa Gardner, University of Minnesota 2:05–2:25 pm Towards whole cells modeled in 3D molecular detail and community curated with cellPack. Graham Johnson, University of California, San Francisco 2:25–2:45 pm Building mechanosensitive artificial cells. Allen Liu, University of Michigan 2:45–3:05 pm Israel reconstitution of contractile actin networks within artificial cells. Kinneret Keren, Technion Israel Institute of Technology, Haifa, Israel 3:05–3:20 pm Break 3:20–3:40 pm 3D mechanics of fast amoeboid cell migration. Juan Carlos Del Alamo, University of California, San Diego 3:40–4:00 pm Spatio-temporal dynamics and metabolic alterations of P53 upon DNA damage. Michelle Digman, University of California, Irvine 4:00–4:20 pm Reliable signal transduction. Roy Wollman, University of California, San Diego 4:20–4:40 pm Cell size dependent mitochondrial functionality in size control. Teemu Miettinen, University of Dundee, Dundee, Scotland 4:40–5:00 pm Noise and robustness in an organelle size control system. Wallace Marshall, University of California, San Francisco
28 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Subgroup C: Cellular and Molecular Mechanobiology: New Approaches, Systems, and Responses Organizers: Morgan Huse, Memorial Sloan-Kettering Cancer Center; Lance C. Kam, Columbia University; Room 33A Bin Chen, Zhejiang University; and Baohua Ji, Beijing Institute of Technology, China SATURDAY
Over the past decade, a number of key technological breakthroughs have emerged that enable investigators to address how physical forces influence cellular physiology. As a result, we have begun to appreciate the importance of mechanosensing in shaping cell fate SATURDAY and function. It is now well established that cells can sense the physical properties of their environment and use this information to guide their proliferative and developmental decisions. Furthermore, there are indications that mechanotransduction serves as a conduit for information transfer between pairs of cells and also among groups of cells within a tissue. We are just beginning to understand how these interactions control systems level behavior in both normal and diseased states. This program will bring together a diverse group of biologists, bioengineers, and physicists to discuss exciting new conceptual and technical advances in mechanobiology. The following four topics will be covered: 1) Mechanosensing in the context of cellular differentiation, homeostasis, and disease, 2) Mechanotransduction during tissue development and repair, 3) Mechanical properties of cell-cell interfaces and their functional consequences, and 4) Novel tools for the analysis of cellular mechanics both in vitro and in vivo. It is our hope that this session will promote wide-ranging discussion of both the key biological questions facing the field and also the technologies that will enable investigators to address them.
Presentations: 1:00–1:10 pm Introduction. Lance Kam, Columbia University 1:10–1:30 pm Force-dependent regulation of alpha-catenin in adherens junction assembly. William Weis, Stanford University 1:30–1:50 pm Mechanotransduction: not just a local affair. Deborah Leckband, University of Illinois 1:50–2:10 pm Nanoscale dynamics of cyto-mechanical transduction triggered by single adhesive ligand bonds to receptors in a spreading cell surface. Evan Evans, Boston University 2:10–2:30 pm Mechanosensing in cell collective behaviors on patterned substrate. Baohua Ji, Beijing Institute of Technology, Beijing, China 2:30–2:50 pm Illuminating the osmotic regulation of wound detection in zebrafish. Philipp Niethammer, Memorial Sloan-Kettering Cancer Center 2:50–3:00 pm Break 3:05–3:25 pm Niche dynamics promote epithelial-mesenchymal transition via mechanical signaling. Adam Engler, University of California, San Diego 3:25–3:45 pm Actin etrograder flow orients and aligns activated, ligand-engaged integrins in focal adhesions. Clare Waterman, National Heart, Lung, and Blood Institute, NIH 3:45–4:05 pm Cooperative unfolding of the GPIbα mechanosensitive and leucine-rich repeat domains transduces signals across platelet membrane. Cheng Zhu, Georgia Institute of Technology 4:05–4:25 pm DNA-based nanoparticle tension probes reveal the role of molecular forces in T cell function. Khalid Salaita, Emory University 4:25–4:45 pm Mechanical potentiation of cytotoxic T cell function. Morgan Huse, Memorial Sloan-Kettering Cancer Center 4:45–5:00 pm Closing Remarks and Open Discussion
Subgroup D: Connexins and Pannexins in Disease Organizer: Dale Laird, University of Western Ontario, Canada Room 16B
It is a general requirement for normal function that adjacent cells within human tissues exchange small molecules through special channels called gap junctions which are assembled from connexins (Cx). In the last couple of decades, mutations in over half of the 21 connexin gene family have been linked to human disease conditions including heart defects, neurodegeneration, skeletal abnormalities, skin disease, stroke, epilepsy, optic disorders and millions of cases of hereditary sensorineural deafness. In the new millennium, a family of three proteins, pannexins, were discovered. These large-pore channel forming proteins share functional overlap with connexin hemichannels in releasing small molecules to the extracellular environment that serve a role in paracrine signaling. Among other roles, pannexins have been described as ATP release channels. Signaling through pannexin channels contributes to seizures under ischemic or epileptic conditions, leads to inflammatory bowel disease, promotes melanoma disease progression and facilitates osteoarthritis.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 29 Presentations: 1:00–1:05 pm Opening Remarks. Dale W. Laird, University of Western Ontario, Canada 1:05–1:20 pm Pannexin 2 localization at ER-mitochondria contact sites sensitizes cells to apoptosis. Maxence Le Vasseur, University of British Columbia, Vancouver, Canada 1:20–1:35 pm ATP evokes pannexin 1 endocytosis to endosomal compartments. Andrew K.J. Boyce, University of Victoria, BC, Canada 1:35–1:50 pm The role of pannexins in fat accumulation and metabolism. Vanessa R. Lee, Western University, London, Ontario, Canada 1:50–2:05 pm Cx46 drives self-renewal and CSC function in glioblastoma. Masahiro Hitomi, Cleveland Clinic 2:05–2:20 pm Targeting gap junction stability to modulate tissue remodeling during pancreas cancer. Joell L. Solan, Fred Hutchinson Cancer Research Center 2:20–2:35 pm Regulation of the connexin 26 gene during epididymal differentiation. Daniel G. Cyr, Institut National de la Recherche Scientifique, Quebec, Canada 2:35–2:50 pm Connexin 43.4 functions as a hemichannel in left-right development in Zebrafish. Jordan M. Welker, Iowa State University, Ames 2:50–3:00 pm Stretch Break Moderator: TBA 3:00–3-15 pm Modeling Cx43-linked pathologies in the human context. Jessica Esseltine, Western University, London, Ontario, Canada 3:15–3:30 pm Alternate translation initiation regulates gap junction losses during epithelial-mesenchymal transition. James W. Smyth, Virginia Tech Carilion Research Institute 3:30–3:45 pm GJA1-20k contributes specificity to gap junction delivery. Shan-Shan Zhang, Cedars-Sinai 3:45–4:00 pm Compartment specific actions of Cx43 and Cx45 in bone modeling and homeostasis. Roberto Civitelli, Washington University in St. Louis 4:00–4:15 pm The cataract related mutant N188T in Cx46 inhibits formation of functional gap junction channels by impairing docking process of Cx46 hemichannels. Anaclet Ngezahayo, Leibniz Universität, Hannover, Niedersachsen, Germany 4:15–4:30 pm Alpha-type Cx43 function is estoredr with 4-PBA treatment in cyctic fibrosis airway epithelial cells that express misfolded F508del-CFTR protein. Samuel A. Molina, Emory University 4:30–4:45 pm Acetylcholine prevents the expression of connexin hemichannels in denervated skeletal myofibers. Juan C. Saez, Pontificia Universidad Católica de Chile 4:45–5:00 pm The gap junction protein, Cx43, forms supramolecular complexes through non-overlapping binding sites for drebrin, tubulin and ZO-1. Cinzia Ambrosi, University of California, San Diego
Subgroup E: Cytoskeletal and Membrane Protein Dynamics at the T Cell Immunological Synapse Organizers: John Hammer, National Heart, Lung, and Blood Institute, NIH;Xufeng Wu, Room 23B National Heart, Lung, and Blood Institute, NIH; and Larry Samelson, National Cancer Institute, NIH
Upon contact with an antigen presenting cell (APC), T lymphocytes undergo rapid reorganizations of their microtubule and actomyosin cytoskeletons. These rearrangements serve to polarize the T cell’s secretory system in the direction of the bound APC and to drive the centripetal movement of T cell receptor and integrin clusters to form the mature immunological synapse. All of these events are required for robust T cell signaling and for the full expression of T cell effector functions. Recent efforts to understand the molecular mechanisms underlying these complex cytoskeletal and signaling phenomena have benefited greatly from the application of three approaches: uper-resolution imaging, in vitro reconstitution, and single-molecule biophysics. This subgroup will focus on recent results gleaned from the application of these three approaches.
1:00–1:05 pm Introduction. John Hammer, National Heart, Lung, and Blood Institute, NIH;Xufeng Wu, National Heart, Lung, and Blood Institute, NIH; andLarry Samelson, National Cancer Institute, NIH 1:05–1:25 pm Negative regulation of T cells: reconstitution and visualization of the PD-1 signaling pathway. Enfu Hui1*, Jing Zhu2, Jeanne Cheung2, Xiaolei Su1, Marcus Taylor1, Jeong Kim2, Ira Mellman2, and Ronald Vale1. 1University of California, San Francisco, 2Genentech Inc. 1:25–1:45 pm Mechanical forces in B cell synapses. Pavel Tolar, The Francis Crick Institute, London, UK 1:45–2:05 pm Structured illumination microscopy of the immune synapse reveals novel insights into actomyosin network formation and function. Srich Murugesan1*, Jinsung Hong1, Jason Yi2, Dong Li3, Lin Shao3, Xufeng Wu1, Eric Betzig3, and John A. Hammer1. 1 National Heart, Lung, and Blood Institute, NIH,2 National Cancer Institute, NIH,3 Janelia Farm HHMI 2:05–2:25 pm Cooperative TCR–pMHC–CD8 catch bond distinguishes thymocyte positive versus negative selection. Jinsung Hong1*, Chenghao Ge1, Ke Bai1, Baoyu Liu1, Loice Chingozha1, Yun Zhang2,
30 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Hang Lu1, Khalid Salaita2, Brian D. Evavold2, Alfred Singer3, and Cheng Zhu 1. 1Georgia Institute of
Technology, 2Emory University School of Medicine, 32National Cancer Institute, NIH, SATURDAY 2:25–2:45 pm Dynamic modulation of cortical actin at the immunological synapse controls cytotoxic granule secretion. Alex T. Ritter1,2*, Gillian M. Griffiths2, and Jennifer Lippincott-Schwartz1. 1National Institute of Child Health and Human Development, NIH,2 Cambridge Institute for Medical Research, Cambridge, UK SATURDAY 2:45–2:55 pm General questions and answers 2:55–3:15 pm Break 3:15–3:35 pm Dynamics and regulation of extracellular vesicle formation in the immunological synapse. Michael L. Dustin, University of Oxford, Oxford, UK 3:35–3:55 pm Multiplexed super-resolution imaging of the immune synapse in T cells. Jason Yi1*, Valarie Barr1, Asit Manna1, Jennifer Hong2, Keir Neuman2, and Lawrence Samelson1. 1National Cancer Institute, NIH, 2 National Heart, Lung, and Blood Institute, NIH 3:55–4:15 pm The nanoscale organization of synaptic actin and NK cell receptors. Daniel M. Davis, anchester Collaborative Center for Inflammation Research, Manchester, UK 4:15–4:35 pm Reconstitution of TCR signaling on model membranes. Jonathon A. Ditlev1,2*, Xiaolei E. Su1,3, Darius V. Köster1,4, Anthony R. Vega2, Enfu Hui1,3, Julia Okrut1,3, Sudeep Banjade1,2, David S. King5, Jack Taunton1,3, Khuloud Jaqaman2, Satyajit Mayor1,4, Ronald D. Vale1,3, and Michael K. Rosen1,2. 1The HHMI Summer Institute, Marine Biological Laboratory,2 University of Texas Southwestern Medical Center, 3University of California, San Francisco, 4National Centre for Biological Sciences, Bangalore, India, 5University of California, Berkeley 4:35–5:00 pm General questions and answers
*Speakers
Subgroup F: Diverse Roles of Glycans and Glycan-Binding Proteins in Human Diseases Organizers: Wei-Sheng Chen, Tufts University; and Christopher J Fisher, University of California, San Diego Room 17A
Glycans, the carbohydrate component of glycoconjugates, are rapidly being considered as a third biological language in addition to nucleic acid and protein sequences. Recent studies have demonstrated that glycans and glycan-binding proteins play a crucial role in the pathogenesis of human diseases including but not limited to tumor metastasis, viral and bacterial infection, cardiovascular disease, and myopathy. Furthermore, glycan-mediated interactions have been shown to be critical in the treatment of disease, and play a particularly important part in vaccine development. In this Special Interest Subgroup meeting, we focus on 1) the fundamental difference between glycan-protein and protein-protein interactions, 2) glycosylation as a dynamic process that modulates several cellular responses, and 3) opportunities and challenges to develop glycan-based therapies. We bring together researchers from different fields of glycobiology to share their views on this specialized, yet broadly impactful area of cell biology research.
Presentations: 1:00–1:03 pm Introduction 1:03–1:26 pm Unusual features of human sialic acid biology: implications for disease. Ajit Varki, University of California, San Diego 1:26–1:49 pm Human disorders of sialic acid synthesis: pathway and prospects for therapy. Marjan Huizing, National Human Genome Research Institute, NIH 1:49–2:12 pm The ‘dark matter’ of cellular signaling: O-GlcNAc and disease epigenetics. John Hanover, National Institute of Diabetes and Digestive and Kidney Diseases, NIH 2:12–2:35 pm Control of COPII vesicle trafficking by intracellular protein glycosylation. Michael Boyce, Duke University 2:35–2:58 pm Glycocalyx engineering to analyze the effects of sialoglycan presentation on early stage influenza infection. Kamil Godula, University of California, San Diego 2:58–3:03 pm Break 3:03–3:26 pm Convergent glycan evolution—meet your inner owl monkey. Pascal Gagneux, University of California, San Diego 3:26–3:49 pm Cancer vaccine development. Chi-Huey Wong, Academia Sinica and Scripps Research Institute 3:49–4:12 pm Glycan-dependent recognition of broadly neutralizing antibodies to HIV: implications for vaccine design. Ian Wilson, Scripps Research Institute
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 31 4:12–4:35 pm Galectin ligand-binding, specificity, mechanism and function. Hakon Leffler, Lund University, Lund, Sweden 4:35–4:58 pm Glycosphingolipids and lectins in fundamental and biomedical research on how to build endocytic pits without clathrin. Ludger Johannes, Institut Curie, Centre de Recherche, Paris, France 4:58–5:00 pm Concluding remarks
Subgroup G: Dynamic Interplay between Lipids, Curvatures, and Diseases of Biological Membranes Organizers: Takanari Inoue, Johns Hopkins University; and Guillaume Thibault, Room 28D Nanyang Technological University, Singapore
The past decade has underscored the enormous complexity of cellular functions, where not only biochemical reactions but also physical properties play critical roles. Among these, biological membranes have emerged as a driving force in which lipid composition and membrane curvature are interconnected and influence numerous biological pathways. Highlighting the importance in biological functions, numerous pathologies have been linked to biological membrane dysregulation. Only recently has a new generation of tools begun to emerge with sufficient power to peer into biochemical and physical membrane properties and dynamics. This special interest subgroup will bring together investigators from different disciplines that have made contributions to these emerging fields. In this session, we will cover molecular and cellular mechanisms regulating biological membranes and cell stress responses to lipid perturbation. In addition, emerging technologies to both visualize and manipulate membrane curvatures will be discussed. Speakers will stress how long-standing and fundamental biological questions are beginning to yield through the application of this new generation of tools. Biological questions will relate to a wide array of vital processes, including endocytosis and exocytosis, cell migration, neuronal plasticity, cellular stress responses, metabolic diseases, etc. Due to the multidisciplinary nature of the studies, the theme targets an extremely diversified audience in the fields of cell biology, biotechnology, nanotechnology, method development, chemical biology, material science, general chemistry and engineering, computational biology, and synthetic biology. A session under this theme will facilitate exchange of ideas among this unusually diverse community, thus offering lively, inspiring opportunities for unconventional research discussions.
Presentations: 1:00–1:05 pm Opening remarks. Takanari Inoue, Johns Hopkins University, and Guillaume Thibault, Nanyang Technological University, Singapore 1:05–1:25 pm Role of membrane-bending proteins in cell polarity formation. Toshiki Itoh, Kobe University, Japan 1:25–1:30 pm Discussion 1:30–1:50 pm ER protein quality control and lipid homeostasis: unexpected functional connections. James Olzmann, University of California, Berkeley 1:50–1:55 pm Discussion 1:55–2:15 pm Membrane bending dynamics during clathrin-mediated endocytosis revealed by pol-TIRF microscopy. Adam Hoppe, South Dakota State University 2:15–2:20 pm Discussion 2:20–2:40 pm Golgi PI4P is a lipid pH biosensor that regulates gene expression and cell growth in response to changes in cytoplasmic pH. Christopher Loewen, University of British Columbia, Canada 2:40–2:45 pm Discussion 2:45–3:05 pm Differential control of actin polymerization dynamics via nanoscale membrane curvature. Milos Galic, University of Münster, Germany 3:05–3:10 pm Discussion 3:10–3:30 pm lpin-1 is necessary for low-PC activation of SBP-1/SREBP-1. Amy Walker, University of Massachusetts Medical School 3:30–3:35 pm Discussion 3:35–3:55 pm Synthetic and physiological approaches to membrane curvatures in cell migration, Allison Suarez, Johns Hopkins University 3:55–4:00 pm Discussion 4:00–4:20 pm Destabilization of a subset of ER transmembrane proteins under lipid disequilibrium. Guillaume Thibault, Nanyang Technological University, Singapore 4:20–4:25 pm Discussion 4:25–4:45 pm F-BAR proteins as driving force for cortical wave propagation. Min Wu, Mechanobiology Institute, National University of Singapore, Singapore 4:45–4:50 pm Discussion 32 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Subgroup H: Extracellular Vesicles - Biogenesis and Function Organizers: David Katzmann, Mayo Clinic; and Tushar Patel, Mayo Clinic Room 29B SATURDAY
Extracellular vesicles (EVs) are secreted by most cell types and have generated interest in the research community due to their impact on physiology. EVs serve as both a mechanism by which a cell can remove unwanted material, but also as a vehicle for cell- cell communication. Recent discoveries pertaining to the biosynthesis and function of EVs are linking these carriers to fundamental SATURDAY aspects of cell and organismal physiology and disease. This Special Interest Subgroup meeting will highlight these developments to ASCB meeting participants and the press, drive collegial interactions within the cell biology and beyond, and provide a forum for critical exchange. Thematic presentations will be followed by an interactive discussion involving all participants pertaining to present and future research areas. This meeting will be supported by the NIH Common Fund supported Extracellular RNA Communication Consortium and the activities and resources available through this consortium will be discussed.
Presentations: 1:00–1:10 pm Introductory remarks. David Katzmann, Mayo Clinic 1:10–1:30 pm Exosomes for the treatment of autoimmune and age-related diseases. Paul Robbins, The Scripps Research Institute, Jupiter, Florida 1:30–1:50 pm Cell-free packaging of microRNAs into exosomes reveals Y-box protein I as a critical sorting factor. Matthew Shurtleff, University of California, Berkeley 1:50–2:10 pm Quantitative proteomic comparison of subtypes of extracellular vesicles: definition of new subfamilies of exosomal and non-exosomal secreted vesicles. Joanna Kowal, Institut Curie, Paris, France 2:10–2:30 pm Extracellular vesicle secretion from apical and basolateral domains of polarized human cholangiocytes. Brian Davies, Mayo Clinic 2:30–2:40 pm Break 2:40–3:00 pm Intercellular oncogenic pathways - pathological biogenesis of extracellular vesicles in cancer. Janusz Rak, McGill University, Quebec, Canada 3:00–3:20 pm KRAS regulation of miRNA sorting into exosomes. Alissa Weaver, Vanderbilt University Medical Center 3:20–3:40 pm A new mode of EGF receptor ligand signaling via exosomes. Robert Coffey, Vanderbilt University Medical Center 3:40–4:00 pm The Leukosome: a biomimetic proteolipid vesicle derived from immune cells. Ennio Tasciotti, The Methodist Research Institute 4:00–4:20 pm The NIH Extracellular RNA Consortium. Tushar Patel, Mayo Clinic 4:20–4:40 pm Future directions in extracellular vesicle research, Fred Hochberg, University of California, San Diego 4:40–5:00 pm Discussion
Subgroup I: Increasing Diversity in a Changing Research Landscape Organizers: Jana Marcette, Harris-Stowe State University; Gary McDowell, Tufts University; Room 17B Tiffany Oliver, Spelman College; and Jessica Polka, Harvard Medical School
In response to a tight funding climate and evolving mechanisms for evaluating, sharing, and conducting research, groups of scientists across the U.S. are now discussing ways to improve the research enterprise. Within this research landscape, the goal of building and retaining a diverse workforce is pressing and necessary. People often hear that minorities are underrepresented in STEM and have their own hypotheses of why this may be. During this session invited speakers will review recent data and efforts to build diversity and respond to the changing landscape of research. This session will include formal presentations and audience-based roundtable discussions to directly address questions, propose solutions, and describe ways we can work to effect change. The session will take the shape of a dialogue rather than a formal lecture as audience input will be welcomed and inform discussion.
Presentations: 1:00–1:10 pm Introduction. Jana Marcette, Harris-Stowe State University with fellow organizers Gary McDowell, Tufts University; Tiffany Oliver, Spelman College; Jessica Polka, Harvard University
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 33 1:10–2:10 pm Increasing diversity in STEM: whose job is it, what needs to be done, and why should we care. Andrew Campbell, Brown University; Tiffany Oliver, Spelman College; Graciela Unguez, New Mexico State University 2:10–2:30 pm Structured Breakout Discussions 2:30–2:45 pm Reports from Breakout Discussions 2:45–3:00 pm Break 3:00–4:10 pm How can policy build a robust and diverse research enterprise? Bill Bement, University of Wisconsin-Madison; Kenny Gibbs, National Cancer Institute, NIH;Gary McDowell, Tufts University; Chris Pickett, American Society for Biochemistry and Molecular Biology (ASBMB) 4:10–4:30 pm Structured Breakout Discussions 4:30–4:45 pm Reports from Breakout Discussions 4:45–5:00 pm Concluding Remarks, Jana Marcette, Harris-Stowe State University
Subgroup J: Microtubule Networks in Differentiated Cells Organizers: Irina Kaverina, Vanderbilt University; Terry Lechler, Duke University; Evelyn Ralston, Room 32B National Institute of Arthritis and Musculoskeletal and Skin Disease, NIH; and Melissa Rolls, Pennsylvania State University
Microtubules form dynamic cytoskeletal networks with diverse functions. We have learned many underlying principles and effectors of microtubule organization from studies in cultured proliferative cells. Differentiation, however, changes cell function and morphology. In many differentiated cells, radial microtubule geometry is lost and non-centrosomal microtubule arrays form. Such differentiation-related microtubule reorganizations have been described for more than 25 years but remain understudied. This subgroup brings together a diverse set of investigators studying aspects of microtubule reorganization, dynamics, and function in differentiated cells and tissues. The goals are to identify both common and divergent mechanisms to regulate microtubule organization, encourage discussion of unexpected functions for microtubules in differentiated cells, and to enable the sharing of reagents for observing/perturbing microtubule networks. We hope that this will connect basic cell biologists with developmental biologists and physiologists to stimulate future studies and collaborations on microtubule functions in tissues.
Presentations: 1:00–1:10 pm Introduction by the Co-chairs 1:10–1:30 pm Microtubule transport in the axon – new frontiers. Peter Baas, Drexel University 1:30–1:50 pm Motors, microtubules and axonal growth Vladimir Gelfand, Northwestern University 1:50–2:10 pm Dendrite branch points are hubs for end-to-end control of microtubules. Melissa Rolls, Pennsylvania State University 2:10–2:30 pm Organization of cortical microtubules in plants. David Ehrhardt, Carnegie Institute 2:30–2:50 pm Control of non-centrosomal microtubule array assembly by end-binding proteins. Karen Oegema, University of California, San Diego 2:50–3:00 pm Break 3:00–3:20 pm Regulation of centrosomal MTOC activity during epidermal differentiation. Terry Lechler, Duke University 3:20–3:40 pm Functions of Golgi-derived microtubules in pancreatic beta cells. Irina Kaverina, Vanderbilt University 3:40–4:00 pm The contribution of perinuclear microtubules to muscle nuclear integrity. Talila Volk, Weizmann Institute 4:00–4:20 pm MAPs and motors cooperate to form the paraxial microtubule array in differentiating muscle cells. Anne Straube, University of Warwick 4:20–4:40 pm Skeletal muscle microtubules: in vitro, in vivo, and in silico exploration of healthy and diseased mammalian muscle. Evelyn Ralston, National Institutes of Heath 4:40–5:00 pm Wrap up and Discussion
34 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Subgroup K: Neuronal Cytoskeleton: Cytoarchitecture and Dynamics
Organizers: Anthony Brown, Ohio State University; Stephanie Gupton, University of North Carolina at Room 33B SATURDAY Chapel Hill; Laura Ann Lowery, Boston College; and Subhojit Roy, University of California, San Diego
Nerve cells extend and maintain long and sometimes elaborately branched processes, axons and dendrites, which define the wiring pattern of the nervous system. This unique and highly polarized morphology is critically dependent on the cytoskeleton, including SATURDAY the specialized and regulated cytoskeletal machinery that generates the dynamic molecular architecture of axonal and dendritic microdomains such as growth cones, synaptic boutons, nodes of Ranvier, axon initial segments, branch points and dendritic spines. This session will highlight exciting findings on the dynamics and cytoarchitecture of the cytoskeleton in these regions of axons and dendrites and their function in axon guidance, extension and regeneration, and in dendritic plasticity and morphogenesis.
Presentations: 1:00–1:05 pm Opening remarks. Anthony Brown, Ohio State University; Stephanie Gupton, University of North Carolina at Chapel Hill; Laura Ann Lowery, Boston College; and Subhojit Roy, University of California San Diego 1:05–1:25 pm Interplay between the cytoskeleton and scaffolds at the axon initial segment. Christophe Leterrier, CNRS, Aix-Marseille Université 1:30–1:40 pm Novel actin organization and dynamics in the axon shaft. Archan Ganguly, University of California, San Diego 1:45–2:05 pm Coordination of axonal cytoskeletal dynamics by mitochondria. Gianluca Gallo, Shriners Hospitals Pediatric Research Center, Temple University 2:10–2:30 pm Regulation of microtubule plus-end dynamics during axon outgrowth. Laura Ann Lowery, Boston College 2:35–2:55 pm A filopodia tabilitys switch for axon guidance. Stephanie Gupton, University of North Carolina Chapel Hill 3:00–3:15 pm Break 3:15–3:25 pm Slit stimulates filopodium formation and elongation to mediate repulsive axon guidance. Russell McConnell, Massachusetts Institute of Technology 3:30–3:50 pm Conditioning drives axon regeneration by Cofilin-mediated actin turnover. Frank Bradke, German Center for Neurodegenerative Diseases (DZNE) 3:55–4:15 pm Cytoskeletal dynamics of the regenerating axon. Andrew Chisholm, University of California, San Diego 4:20–4:30 pm The Down syndrome critical kinase, Minibrain/Dyrk1a, controls dendrite morphogenesis through direct regulation of the microtubule cytoskeleton. Kassandra Ori-McKenny, University of California, Davis 4:35–4:55 pm A new cytoskeletal model for trafficking material to dendritic spines during synaptic plasticity. Erik Dent, University of Wisconsin, Madison
Subgroup L: Nuclear Envelope Dynamics Organizers: Dennis Discher, University of Pennsylvania; Harald Herrmann, German Cancer Research Center (DKFZ); Room 28B Megan King, Yale University; Patrick Lusk, Yale University; and Katherine Wilson, Johns Hopkins University
This Subgroup will celebrate important advances in understanding the composition, structure, and function of the nuclear envelope, including the nuclear lamina. Speakers will present insights into how lamins impact genome organization and modulate nuclear mechanics. A major theme will be to explore mechanisms that support nuclear integrity despite dynamic nuclear envelope remodeling via ‘machines’ that contribute to nuclear envelope breakdown and reformation, nuclear pore biogenesis and “mega” RNP egress.
Presentations: 1:00–1:05 pm Welcome – Organizing committee 1:05–1:20 pm The assembly of A- and B-type lamins: polyelectrolyte-default and type-specific optional pathways. Harald Herrmann, Deutsches Krebsforschungszentrum, DKFZ/Heidelberg, Germany 1:20–1:35 pm A remarkable complex of interwoven lamin meshworks is revealed by super-resolution microscopy. Robert Goldman*, Northwestern University Feinberg School of Medicine; Takeshi
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 35 Shimi, Northwestern University; Mark Kittisopikul, University of Texas Southwestern Medical Center; Joseph Tran, Carnegie Institution for Science; Anne E. Goldman, Northwestern University; Stephen A. Adam, Northwestern University; Yixian Zheng, Carnegie Institution for Science; Khuloud Jaqaman, University of Texas Southwestern Medical Center 1:35–1:50 pm The cell biology of lamins in development. Yixian Zheng, Carnegie Institute 1:50–2:05 pm Metabolic regulation of lamin A. Katherine Wilson, Johns Hopkins University 2:05–2:20 pm Regulation of signaling by inner nuclear membrane proteins. Larry Gerace, Scripps 2:20–2:35 pm Functional enomeg organization at the nuclear lamina. Karen Reddy, Johns Hopkins University 2:35–2:50 pm Lamin A mechanosensing: regulation and regulator. Dennis Discher, University of Pennsylvania 2:50–3:00 pm Break 3:00–3:15 pm Coupling nuclear structures to the cytoskeleton. Brian Burke, IMB Singapore 3:15–3:30 pm Mechanical crosstalk between the nucleus and adhesions. Megan King, Yale University 3:30–3:45 pm The mechanism of Torsin ATPase activation and its dysfunction in primary dystonia. Christian Schlieker, Yale University 3:45–4:00 pm mRNP remodeling through the NE. Vivian Budnik, University of Massachusetts Medical School 4:00–4:15 pm Losing integrity: nuclear envelope rupture in interphase. Emily Hatch* and Martin Hetzer, The Salk Institute for Biological Studies. 4:15–4:30 pm Nuclear envelope rupture and repair during cell migration in confined 3-D environments. Jan Lammerding, Cornell University 4:30–4:45 pm ESCRTs and nuclear envelope reformation. Jeremy Carlton, King’s College, London 4:45–5:00 pm Quality control at the nuclear envelope. Patrick Lusk, Yale University
*Speaker
Subgroup M: Nucleation Phenomena in Cell Biology Organizers: Clifford Brangwynne, Princeton University; Gary Brouhard, McGill University, Montreal, Canada; Room 29C and Xiaolei Su, University of California, San Francisco
This Special Interest Subgroup will bring together researchers working on the nucleation of a variety of intracellular structures. Nucleation is a critical step in the de novo assembly of cytoskeletal polymers from monomeric subunits and also in the assembly of liquid-phase intracellular structures, including RNA/protein bodies in the cytoplasm and nucleus as well as cell surface micro- clusters. The biophysical mechanisms that drive nucleation phenomena in cell biology remain poorly understood. The speakers in this session will include leaders in the established field of cytoskeletal nucleation (actin filaments and microtubules) as well as leaders in the emerging field of phase transitions. The overarching goal of the session will be to establish the fundamental principles by which cells govern nucleation across systems.
Presentations: 1:00–1:10 pm Introductory Remarks. Gary Brouhard, McGill University 1:10–1:30 pm Phase separation of signaling molecules promotes T cell activation. Xiaolei Su, University of California, San Francisco 1:30–1:50 pm RNA transcription modulates phase transition-driven nucleolar assembly. Stephanie Weber, Princeton University 1:50–2:10 pm Assembly and dynamics of stress granules. Roy Parker, University of Colorado, Boulder 2:10–2:30 pm Mechanism(s) of actin nucleation essential to oogenesis. Margot Quinlan, University of California,Los Angeles 2:30–2:50 pm Actin nucleation: what do we really know? Bruce Goode, Brandeis University 2:50–3:10 pm Break 3:10–3:30 pm A minimal microtubule organizing center. Trisha Davis, University of Washington 3:30–3:50 pm Microtubule quaternary structure and nucleation. Gary Brouhard, McGill University 3:50–4:10 pm From branching microtubule nucleation to mitotic spindle assembly: role and mechanism. Sabine Petry, Princeton University 4:10–4:30 pm Cajal bodies shape genome conformation. Miroslav Dundr, Rosalind Franklin University 4:30–4:50 pm Phase transitions in intracellular organization and disease. Simon Alberti, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany 4:50–5:00 pm Concluding Remarks
36 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Subgroup N: Polymerizing Enzymes: New Frontiers in Protein Compartmentalization and Localization
Organizers: Justin Kollman, University of Washington; Ji-Long Liu, University of Oxford, UK; Room 30B SATURDAY and Jeffrey Peterson, Fox Chase Cancer Center
An increasing number of enzymes have been discovered to dynamically and reversibly assemble into macroscopic filaments in
response to changes in nutrient availability or other environmental cues. These filamentous structures represent a novel non- SATURDAY membrane-bound mechanism for compartmentalization and localization of enzymatic activity. Most enzyme filaments discovered to date are metabolic enzymes (e.g., CTP synthase, inosine monophosphate dehydrogenase, and phosphofructokinase), but other enzyme classes are also represented. The discovery of numerous enzyme filaments opens an exciting new field at the interface between cell biology and metabolism. Most enzyme filaments remain uncharacterized, but strong evolutionarily conservation of polymerization, from bacteria to humans, suggests the filamentous forms play fundamentally important functional roles. Where functional data do exist it is clear that the filaments are important for regulating enzyme activity and for maintaining cellular homeostasis. Important fundamental questions remain for many enzyme filaments: What are their structures and mechanisms of assembly? What is their biological function? How is their assembly regulated? The presentations in this subgroup will discuss recent advances in addressing these questions, both in vitro and in vivo, for a variety of polymerizing enzymes.
Presentations: 1:00–1:10 pm Introduction and Welcome 1:10–1:30 pm TBD. Tim Mitchison, Harvard Medical School 1:30–1:50 pm Function and egulationr of cytoplasmic filaments of CTP synthase and IMPDH. Jeffrey Peterson, Fox Chase Cancer Center 1:50–2:10 pm Regulators of cytoophidium assembly. Ji-Long Liu, University of Oxford, UK 2:10–2:30 pm Visualizing CtpS assembly as a single-cell reporter of metabolism. Zemer Gitai, Princeton University 2:30–2:50 pm Quinary protein structure as regulator of cytoplasmic organization. Simon Alberti, Max Planck Institute of Molecular Cell Biology and Genetics, Germany 2:50–3:15 pm Break 3:15–3:35 pm The structural basis for CTP synthase filament assembly. Justin Kollman, University of Washington 3:35–3:55 pm Redox regulation of E. coli CTP synthetase. Enoch Baldwin, University of California, Davis 3:55–4:15 pm One-carbon metabolism on IMPDH assembly into rod/ring structures. Edward Chan, University of Florida 4:15-4:35 pm Identification of a metabolic actin: building a bridge between metabolism and the classical cytoskeleton. James Wilhelm, University of California, San Diego 4:35-4:55 pm Glucokinase polymerization may regulate glycolytic flux during environmental transitions. Ethan Garner, Harvard University 4:55-5:00 pm Closing remarks
Subgroup O: Quantitative Microscopy and Image Analysis: Measuring Cellular Organization and Dynamics Organizers: Hunter Elliott, Harvard Medical School; Talley Lambert, Harvard Medical School; Room 30D Thomas L. Schwarz, Boston Children’s Hospital and Harvard Medical School; Evgeny Shlevkov, Boston Children’s Hospital and Harvard Medical School; and Jennifer Waters, Harvard Medical School
With the technological advances of the past two decades, modern optical microscopy offers tremendous analytical power. When careful image acquisition is paired with rigorous computational analysis, the potential exists to extract biological information at high resolution and precision, without bias and subjectivity. Methods such as high content screening, single molecule imaging and the latest advances in high resolution and high speed live imaging (e.g., lattice light sheet microscopy) rely on the development of complementary image analysis methods to allow the effective derivation of comprehensive descriptions of cellular organization and dynamics. Quantitative microscopy therefore often requires a collaborative effort across multiple disciplines, from biology, to physics, to computer science. This session highlights researchers from across these disciplines doing exemplary work in the fields of quantitative microscopy and image analysis.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 37 Presentations: 1:00–1:05 pm Introduction to Part 1, Organizers 1:05–1:35 pm Examining anaphase dynamics in human cells using lattice light sheet microscopy. Tarun Kapoor, Rockefeller University 1:35–2:05 pm Quantitative approaches to unravel the molecular mechanisms of clathrin-mediated endocytosis. Julien Berro, Yale University 2:05–2:35 pm Forcing cells into shape: using optogenetics and micropatterns to measure cellular mechanics. Patrick Oakes* and Margaret Gardel, University of Chicago 2:35–2:55 pm Break 2:55–3:00 pm Introduction to Part 2, Organizers 3:00–3:30 pm Quantitative single-molecule analysis of receptor dynamics and interactions. Khuloud Jaqaman, University of Texas Southwestern Medical Center 3:30–4:00 pm Multiparametric analysis of particle transport for high-throughput screening. Evgeny Shlevkov, Boston Children’s Hospital and Harvard Medical School 4:00–4:30 pm Systems genetics and cell biology: mapping biological pathways in budding yeast. Brenda Andrews, University of Toronto 4:30–5:00 pm Building models of cell organization, differentiation and perturbation directly from microscope images. Robert F. Murphy, Carnegie Mellon University
*Speaker l Undergraduate Program 2:15-3:15 pm Ballroom 6A
Translating Curiosity: Solving the Worm Paul W. Sternberg California Institute of Technology
In this discussion targeted to undergraduates, Paul Sternberg will discuss vignettes from his 30 years of work withCaenorhabditis elegans (The Worm) to illustrate how curiosity-driven science leads to translatable results. He will also discuss the prospects and challenges in trying to comprehend an organism in its entirety, with an eye toward our own biology. Sternbeg will undoubtedly make a series of tangential points relevant to your future as a scientist and a person. Organized by the ASCB Education Committee
l Poster Competition and Reception 3:30-5:30 pm Ballroom 6A
This session is optional for all undergraduates who submit an abstract by October 14 for the Annual Meeting and is required for all those receiving travel awards from the Minorities Affairs Committee. This session allows students to practice presenting their research posters before their main poster presentation in the ASCB Learning Center. Winners will receive cash awards. Everyone attending the meeting is welcome to stop by! Organized by the ASCB Minorities Affairs and Education Committees
38 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l High School Program: Exploring the Microscopic World with Your Own Foldscope
4:00-5:30 pm Room 5B SATURDAY SATURDAY
Manu Prakash Stanford University What if every person in the world could carry a microscope around in his or her pocket? That is the idea behind the Foldscope, a 50-cent print-and-fold mass-produced paper microscope. Come build a Foldscope from scratch with developers from the Prakash Lab and ASCB scientist volunteers. Explore the hidden treasures of the microscopic world around you and gain a new perspective for the invisible world that makes everything tick.
l Meet and Greet 5:00-5:45 pm Room 24A-C All are welcome, especially first-timers. Come to network, and hear from some of the ASCB leadership. A cash bar and light refreshments will be available.
l Keynote Symposium 6:00 pm Ballroom 20BC
Can the Science Paradox be Resolved? Tiny Cell, Global Impact: A Journey of Discovery with a Microbe from the Sea Photo credit: Joy Leighton Photo credit: Richard Howard
Sallie W. Chisholm The Honorable, Professor Jane Lubchenco Massachusetts Institute of Technology Oregon State University
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 39 l Posters on Display 6:00-8:00 pm Learning Center
l Opening Night Reception Immediately following Keynote–10:00 pm Sails Pavilion
Join us in celebrating the start of another great meeting! Meet new people, find old friends and colleagues, and start having fun. All registered meeting attendees and exhibitors are invited to the buffet reception. Cash bar available. l International Research and Training Exchange Fair 8:00-9:30 pm Sails Pavilion
Coordinator: Cynthia Jensen, University of Auckland
The fair will allow attendees to learn about research, training, and other opportunities in countries around the world; encourage students and postdocs to think about possibilities in other countries; and open up exchanges between labs for international collaboration. Tables will be set up displaying information from various countries and regions around the world, and representatives will be available to answer questions. Make sure to check out this event while you enjoy refreshments and collegiality during the Opening Night Reception! Organized by the ASCB International Affairs Committee
40 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Sunday December 13, 2015
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 41 7:30 am-6:30 pm Registration Open Registration Area 7:30 am-8:00 pm Career Center Open Learning Center 8:00 am-9:30 am Symposium 1 Ballroom 20BC Pushing the Limits: Visualization of Hidden Biological Processes 8:00 am-12:00 pm Wikipedia Edit-a-Thon Room 31A
9:00-10:25 am Delivering Science: Effective Communication Skills to Career Center Theater, Become a Successful Scientist Learning Center 9:30-4:30 pm ASCB Learning Center (Exhibit Hall) Open 9:30-10:30 am Table Talk Roundtable Central Section 2, Strategies for Teaching Science Writing and Literature Reading Learning Center
9:30-10:30 am Exhibitor Tech Talk Theater 1, Learning Center Nanolive SA: Nanolive launches the first holographic tomographic microscope in the world: the 3D Cell Explorer Lipotype GmbH: An automated shotgun lipidomics platform for high throughput, comprehensive, and quantitative analysis of lipids from biological and clinical samples Open Imaging, Inc.: Open source image acquisition with μManager 9:30-10:30 am Exhibitor Tech Talk Theater 2, Learning Center Cell Signaling Technology: Simple assays for cellular analyses 9:30-11:30 am Morning Refreshment Break Learning Center 9:45-10:45 am Symposium 2 Ballroom 20BC Wisdom of Crowds: Collective Decision-Making by Cells and Organisms 10:00-12:00 pm Foundational Cell Biology Workshop: Making BIG Data Accessible Room 33B for Teaching and Learning 10:30-11:25 am Making the Most of the Annual Meeting Career Center Theater, Learning Center 10:45-11:45 am Exhibitor Tech Talk Theater 2, Learning Center eBioscience, an Affymetrix Business: Single-cell gene expression in context 10:45-11:45 am Exhibitor Tech Talk Theater 1, Learning Center Thermo Fisher Scientific: New transfection reagents for CRISPR editing and in vivo applications 11:00 am-12:00 pm Talking about Evolution with Doubters: Practical Tips Room 32B 11:00 am-12:00 pm Exhibitor In-Booth Presentation Booth 1235/1237 National Institutes of Health (NIH): National Institute of General Medical Sciences (NIGMS) and Center for Scientific Review (CSR) Daily Schedule—Sunday, December 13 11:00 am-12:00 pm Science Discussion Tables Roundtable Central Section 3, Learning Center 11:00 am-12:00 pm E.E. Just Lecture Room 24B Erich D. Jarvis 12:00-1:30 pm Odd-Numbered Poster Presentations Learning Center 12:00-1:10 pm Microsymposium 1: Cell Motility and Migration Microsymposia Room 1, Learning Center 12:00-1:10 pm Microsymposium 2: Signaling in Health and Disease Microsymposia Room 2, Learning Center 12:00-12:45 pm Exhibitor Tech Talk Theater 2, Learning Center VitaCyte: Collagenase: moving from black box to transparent knowledge 12:00-12:45 pm Exhibitor Tech Talk Theater 1, Learning Center EMD Millipore: Cell cycle and cell death: studying the connections with simplified cytometry 12:00-12:55 pm MD-PhD, Is It Right for Me? Career Center Theater, Learning Center 12:00-1:00 pm Table Talk Roundtable Central Section 1, Foldscopes Learning Center 12:00-4:00 pm Afternoon Refreshment Break Learning Center
42 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 12:30-1:30 pm Table Talk Roundtable Central Section 3, Lessons Learned about Starting a New Lab Learning Center 12:30-1:30 pm Table Talk Roundtable Central Section 2, National Research Mentoring Network Learning Center 12:30 pm Exhibitor In-Booth Presentation Booth 923 QImaging: You can have it all: new cameras, new advanced features, new software 1:00-1:55 pm Startups and Scientists: Our Strengths and Weaknesses as Entrepreneurs Career Center Theater, Learning Center 1:00-2:45 pm Exhibitor Tech Talk Theater 1, Learning Center Carl Zeiss Microscopy, LLC: Accessing the emerging imaging technologies at HHMI Janelia Research Campus Carl Zeiss Microscopy, LLC: New acquisition and detection modes with ZEISS Airyscan Carl Zeiss Microscopy, LLC: AiryScan: bringing super resolution to confocal microscopy Daily Schedule—Sunday, December 13 1:00-1:45 pm Exhibitor Tech Talk Theater 2, Learning Center Bruker Nano Surfaces: Advances in live super-resolution imaging using the Vutara 352 microscope 1:00-2:00 pm Exhibitor In-Booth Presentation Booth 1235/1237 National Institutes of Health (NIH): National Institute of General Medical Sciences (NIGMS) and Center for Scientific Review (CSR) 1:00-1:20 pm Film: The Biology of Skin Color ASCB Booth (721) Theater, Learning Center 1:15 pm Exhibitor In-Booth Presentation Booth 934 Abcam, Inc: Immunostaining: from sample prep through troubleshooting and beyond 1:15 pm Exhibitor In-Booth Presentation Booth 921 Photometrics: Imaging with signal restoration super powers 1:25-2:35 pm Microsymposium 3: Membrane Dynamics and Visualization Microsymposia Room 1, Learning Center 1:25-2:35 pm Microsymposium 4: Cell Division and Cytokinesis Microsymposia Room 2, Learning Center 1:30-2:15 pm Meet the Editor of CBE—Life Sciences Education ASCB Booth (721), Learning Center 1:30-2:30 pm Table Talk Roundtable Central Section 2, Incorporating Research into Lab Courses— Learning Center Reports from REIL Biology 1:30-2:30 pm Table Talk Roundtable Central Section 3, Using Cutting-Edge Models in the Classroom Learning Center 1:30-3:00 pm Even-Numbered Poster Presentations Learning Center 2:00 pm Exhibitor In-Booth Presentation Booth 320 Nanomedical Diagnostics: AGILE Research Biosensor live demonstration with complimentary beer 2:00-3:00 pm International Affairs Committee (IAC) Roundtable Roundtable Central Section 1, Learning Center 2:00-2:45 pm Exhibitor Tech Talk Theater 2, Learning Center Nikon Instruments, Inc.: Illuminating biology with super-resolution microscopy 2:00-2:55 pm Career Panel: Science Policy Career Center Theater, Learning Center 2:00 pm Exhibitor In-Booth Presentation Booth 923 QImaging: You can have it all: new cameras, new advanced features, new software 2:50-4:00 pm Microsymposium 5: Mechanics in Cellular Maintenance and Disease Microsymposia Room 1, Learning Center 2:50-4:00 pm Microsymposium 6: Studying Organelle Function: Microsymposia Room 2, New Trends and Technologies Learning Center
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 43 3:00-3:50 pm Science Discussion Tables Roundtable Central Section 3, Learning Center 3:00-4:00 pm Exhibitor Tech Talk Theater 2, Learning Center Bitplane Inc.: Cell lineage analysis – Imaris 8.2 launch at ASCB 2015 3:00-4:00 pm WICB Network Reception Goes International Room 33C 3:00-4:00 pm Career Panel: Consulting and Entrepreneurship Career Center Theater, Learning Center 3:00-4:00 pm HHMI Biointeractive Animations ASCB Booth (721), Learning Center 3:00-4:00 pm Exhibitor In-Booth Presentation Booth 335 3H Biomedical AB: Characterization and large scale expansion of human satellite cells 3:00-6:30 pm Large-Scale Data Workshop: Computational Methods for RNA Sequencing Analysis Room 31B 3:15-3:45 pm Bruce Alberts Award for Excellence in Science Education Room 24B Deborah Harmon Hines 4:00-6:25 pm Multicellular Interactions, Tissues, and Development Ballroom 20D Minisymposium 1: Cell Migration in Tissues
Signaling and Differentiation Room 30C Minisymposium 2: Cellular Decision-Making
Cell Cycle and Cell Division Room 29C Minisymposium 3: Chromosome Segregation
Cell Biology of Genetic Information Room 28D Minisymposium 4: Genome Organization and Stability
Membrane Organization, Dynamics, Traffic, and Regulation Ballroom 20A Minisymposium 5: Mechanisms for Shaping Membranes Cytoskeleton, Motility, and Cell Mechanics Ballroom 20C Daily Schedule—Sunday, December 13 Minisymposium 6: Molecular Motors and the Cytoskeleton: Measurement, Manipulation, and Mechanics
Education Minisymposium: Teaching How to Teach and Learn Room 32B
4:15-5:15 pm Exhibitor Tech Talk Theater 2, Learning Center Horizon Discovery: Genome editing in human cells using CRISPR/Cas technology 6:45 pm-8:00 pm ASCB Kaluza Prize Presentation and Keith R. Porter Lecture by Ballroom 20BC Jonathan S. Weissman
44 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Sunday, December 13 l Career Center 7:30 am-8:00 pm Learning Center Stop by any time to check out the job postings.
9:00 am-4:00 pm One-on-One CV Review Drop in and have an experienced ASCB member help you hone the perfect CV. SUNDAY Personalized Career Coaching Drop by to talk one-on-one with life sciences career experts about your personal career trajectory. Immigration Consultation Stop by for a free individual immigration consultation from experts from Getson & Schatz.
l Symposium 1: Pushing the Limits: Visualization of Hidden Biological Processes 8:00-9:30 am Ballroom 20BC
Supported by Biology of the Cell, Wiley Chair: Jennifer Lippincott-Schwartz, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH
8:00 am S1 In vivo imaging of cellular dynamics from the nanoscale to the macroscale. E. Betzig1; 1Janelia Research Campus, Ashburn, VA 8:30 am S2 Illuminating biology at the nanoscale with single-molecule and super-resolution fluorescence microscopy. X. Zhuang1; 1Department of Chemistry and Chemical Biology, HHMI/Harvard University, Cambridge, MA 9:00 am S3 The story of single molecules, from early spectroscopy in solids, to super-resolution microscopy, to 3D dynamics of biomolecules in cells. W. Moerner1; 1Chemistry, Stanford University, Stanford, CA
l Wikipedia Edit-a-Thon 8:00 am-12:00 pm Room 31A
Supported by the Simons Foundation Please pre-register for this event at http://goo.gl/forms/TXyiaA5WZV Wikipedia is the fifth most popular website in the world, boasting 18 billion page views per month. Because of the digestible nature of the articles, it is often the first resource students, teachers, researchers, and the general public check to learn about new material. Using this existing resource, ASCB can disseminate correct scientific information to the masses with a low cost-to-impact ratio. The edit-a-thon will empower cell biologists with the skills necessary to contribute to Wikipedia with their authoritative expertise in their research field. It is our hope that this event will inspire members to continue to edit the encyclopedia as they advance the frontiers of science.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 45 l Delivering Science: Effective Communication Skills to Become a Successful Scientist 9:00-10:25 am Career Center Theater, Learning Center
Veronica Segarra, PhD, Assistant Professor of Biology, High Point University, and Mónica I. Feliú-Mójer, PhD, Program Manager at iBiology
During this interactive workshop, participants will learn and put into practice effective communication skills to support their professional advancement. The session will provide participants with 1) an introduction of effective communication principles and strategies; 2) tangible examples of how effective communication skills can support their professional advancement; and 3) resources that will help them strengthen their communication skills. Discussion will include communicating science to different audiences (e.g., peers versus general audience) and the challenges, opportunities, and advantages of incorporating effective communication skills into their toolbox. Scientists and professionals at all stages in their career are encouraged to attend. Organized by the ASCB Committee for Postdocs and Students (COMPASS) l ASCB Learning Center (Exhibit Hall) Open 7:30 am-8:00 pm Exhibits open 9:30 am-4:00 pm. l Table Talk 9:30-10:30 am Roundtable Central Section 2, Learning Center
Strategies for Teaching Science Writing and Literature Reading Jennifer Hood-DeGrenier, Worcester State University l Exhibitor Tech Talk 9:30-10:30 am Theater 1, Learning Center
9:30-9:50 am, Nanolive SA: Nanolive launches the first holographic tomographic microscope in the world: the 3D Cell Explorer Presenter: Lisa Pollaro Level: Intermediate This year at ASCB2015, Nanolive (Booth #1322) will launch its revolutionary microscope, the 3D Cell Explorer, which images living cells instantly, in 3D and 4D (http://goo.gl/mtcm7o), without labels at a resolution below the diffraction limit of light (200nm; http:// nanolive.ch/3d-cell-explorer/). The refractive index distribution within the cell is measured at each pixel and the researcher can decide which parts of the cell to visualize by digitally staining them in contrasting colors, without interfering with the cell’s normal physiology (http://nanolive.ch/technology/). Don’t miss our launch event on Monday 5:30-7:45 pm in Theater 2, Learning Center (http://www.ascb.org/2015meeting/tech- launches).
9:50-10:10 am, Lipotype GmbH: An automated shotgun lipidomics platform for high throughput, comprehensive, and quantitative analysis of lipids from biological and clinical samples Presenter: Dr. Christian Klose Level: Intermediate Complex lipid compositions of a variety of samples, like tissues, cells, cellular preparations, organelles, body fluids, and others are challenging to analyze quantitatively in a single, straightforward measurement. Here we present a fully quantitative lipidomics technology characterized by high precision not only on different days but also in different laboratories. At the same time our technology allows for high lipid coverage, without compromising the throughput. To scrutinize the feasibility of our approach we applied it to human blood plasma, where it proved: 1) to be comprehensive, covering 22 different lipid classes encompassing more than 200 lipid species; 2) to be high-throughput amenable, allowing for the analysis of hundreds samples per day; 3) to achieve absolute quantification of individual lipid molecules, by inclusion of lipid class-specific internal standards.
46 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 10:10-10:30 am, Open Imaging, Inc.: Open source image acquisition with µManager Presenter: Mark A. Tsuchida Level: Introductory µManager (Micro-Manager) is free and open source software for optical microscope control and image acquisition. µManager allows you to run imaging experiments using a wide range of devices including major microscope stands, scientific cameras, stages, illuminators, and more, all through a simple and intuitive interface. In this short presentation, we will introduce you to µManager’s features and capabilities and provide a glimpse into µManager’s extensibility and customizability. We will also discuss why you should care about open source software for science and introduce the µManager user support services available from Open Imaging. l Exhibitor Tech Talk SUNDAY 9:30-10:30 am Theater 2, Learning Center
Cell Signaling Technology: Simple assays for cellular analyses Presenter: Dr. Randall K. Wetzel, PhD Level: Intermediate Fluorescently labeled antibodies and cellular dyes can be combined with simple cellular analysis devices to quickly and easily monitor biological processes. These include cell health, viability, immune or other cellular signaling, protein expression, metabolism, cell cycle, apoptosis, and toxicity in multiplex whole cell or lysate-based sandwich assays. In this presentation, we will review common cellular assays and describe simple protocols and reagents to enable these assays. l Morning Refreshment Break 9:30-11:30 am Learning Center
Join us for complimentary coffee and tea while visiting exhibitors and viewing posters. l Symposium 2: Wisdom of Crowds: Collective Decision-Making by Cells and Organisms* 9:45-10:45 am Ballroom 20BC Supported by Sanofi Chair: Alpha Yap, University of Queensland, Brisbane, Australia
9:45 am S4 Collective cell migration: the power of many. R. Mayor1; 1Cell and Developmental Biology, University College London, London, UK 10:15 am S5 The ecology of collective behavior. D.M. Gordon1; 1Biology, Stanford University, Stanford, CA
*Heinz Hermann endowed Symposium. Heinz Hermann was Professor Emeritus of Molecular and Cell Biology at the University of Connecticut. A symposium in his honor was endowed at the ASCB in 1990. A founder of the ASCB, Professor Hermann was well known for his pioneering approach to research in developmental biology, which led to over 100 publications. He also wrote two books—Cell Biology and From Biology to Sociopolitics.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 47 l Foundational Cell Biology Workshop: Making BIG Data Accessible for Teaching and Learning 10:00-12:00 pm Room 33B
Molly Bolger Anne Rosenwald Irina Makarevitch Caroline Kane John Albeck University of Arizona Georgetown Hamline University University of California, University of California, University Berkeley Davis Supported by Howard Hughes Medical Institute (HHMI) The session will begin with a 10-minute introduction to HHMI BioInteractive educational resources that illuminate scientific process and impart the thrill of scientific discovery. Based on real data and highlighting research practices, HHMI’s short films, virtual labs, data activities, apps and print materials combine important science with engaging presentation. These multimedia resources are developed, vetted, and field-tested by educators and scientists, and available for free on BioInteractive.org. In keeping with the Big Data theme running through this meeting, this workshop will engage participants with approaches to integrate the generation and/or analysis of Big Data in the undergraduate curriculum. During the first hour, participants will hear about models that span genomics, image analysis and network analysis in signal transduction that utilize research papers and research projects as starting points to enable students to understand core biological concepts. During the second hour, small group discussions will focus on subtopics such as the teaching space (large vs small class sizes), the challenges of Big Data analysis in the classroom, faculty training, and making such efforts accessible to a cross-section of educators, including those at two-year colleges, primarily undergraduate teaching institutions, and high schools, community colleges, high schools, etc. Program Officers from NIH and NSF will be available to discuss funding opportunities in this area. Organized by the ASCB Education Committee
l Making the Most of the Annual Meeting 10:30-11:25 am Career Center Theater, Learning Center
Natalie Lundsteen, PhD, Assistant Professor of Psychiatry and Director of Graduate Career Development, Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center
You made it to San Diego. Now—create a strategy to maximize your time and opportunities. Over the next few days, you will be able to build your science knowledge but you also can grow your network of contacts, learn about potential career fields, and maybe, just maybe, start a conversation that could lead to a fantastic research collaboration or even a job offer. In this session we will discuss tips and tricks for taking advantage of all kinds of annual meeting situations and interactions, including how to make a great impression, what to ask employers and industry reps, practice delivering introductions, and planning for follow-up communication. Please bring your questions, a positive attitude, and something to take notes! Organized by the ASCB Committee for Postdocs and Students (COMPASS) l Exhibitor Tech Talk 10:45-11:45 am Theater 2, Learning Center
eBioscience, an Affymetrix Business: Single-cell gene expression in context Presenter: Matthew H. Cato, Applications Scientist Level: Intermediate Tools to analyze single cells for their discrete characteristics are becoming increasingly mainstream, bringing with it significant scientific momentum. Scientists understand that cell populations are heterogeneous and bulk measurements can mask events when using the law of averages. However, current genomics approaches introduce variables that can result in over/under representation
48 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting of certain cell types. Ultra-sensitive fluorescent in situ hybridization (FISH) that relies on signal amplification rather than transcript amplification provides an outstanding single-cell validation tool. These techniques illustrate subcellular, population dynamics/cell frequencies, or indicate morphological context. ViewRNA® and PrimeFlow® FISH platforms for microscopy and flow cytometry incorporate a proprietary probe design using branched DNA (bDNA) signal amplification technologies that result in excellent specificity, low background, and high signal-to-noise ratios.
l Exhibitor Tech Talk 10:45-11:45 am Theater 1, Learning Center
Thermo Fisher Scientific: New transfection reagents for CRISPR editing and in vivo applications SUNDAY Presenter: Xavier de Mollerat du Jeu, PhD, Director R&D Life Science Level: Intermediate Xavier de Mollerat du Jeu is leading the R&D efforts for the development of new delivery solutions of Nucleic acids, including Lipofectamine® and Invivofectamine® product lines. During this seminar he will focus on the latest advances in: • Nucleic acid delivery solutions for hard-to-transfect and Primary cells • Delivery of Genome editing tools, including cas9 protein • High titer Lentiviral production solutions • In vivo delivery of RNAi and mRNA using invivofectamine3.0
l Talking about Evolution with Doubters: Practical Tips 11:00 am-12:00 pm Room 32B
Ann Reid National Center for Science Education
What do you say when someone says “I don’t believe in evolution”? Faced with such a statement, some biologists mutter something vague about evolution being well-established and quickly change the subject. Others mentally push up their sleeves, put up their dukes, and prepare for a data-driven debate. Neither strategy is particularly productive if the goal is to help someone understand why evolution is such a profoundly important theory, and why “not believing” in it is not a scientific option. Whether you’re keen to engage or anxious to avoid, with some practice, you can enter into such conversations with a greater chance of success in helping increase the public understanding of science. The National Center for Science Education, founded over 25 years ago to defend the teaching of evolution in public schools, has long experience communicating with people who reject evolution, or think it scientifically controversial. During this session, we will discuss some of the most common reasons individuals reject or question evolution and provide suggestions about how to engage such individuals in a productive way. Be prepared for hands-on practice! Organized by the ASCB Education Committee l Exhibitor In-Booth Presentation 11:00 am-12:00 pm Booth 1235/1237
National Institutes of Health (NIH): National Institute of General Medical Sciences (NIGMS) and Center for Scientific Review (CSR) Presenters: CSR Scientific Review Officers and NIGMS Program Directors Meet NIH Cell Biology Integrated Review Group Scientific Review Officers and NIGMS Program Directors
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 49 l Science Discussion Tables 11:00 am-12:00 pm Roundtable Central Section 3, Learning Center
Whether you’re a student, postdoc, or PI, ASCB will again offer special networking opportunities with senior scientists and peers. Select your interest area and bring your questions to the ASCB Learning Center in the San Diego Convention Center.
Table No. Presenter Topic 1 Bruce Alberts Science education or science policy 2 Pietro De Camilli How to start an independent lab 3 Jennifer Lippincott-Schwartz Organelle dynamics/crosstalk and superresolution 4 David Drubin Cytoskeleton 5 Anthony Hyman Phase transition in cytoplasm 6 Richard McIntosh Mitosis and microtubule 7 Thoru Pederson Cell biology of CRISPR 8 David Spector Nuclear organization and function 9 Claire Walczak Mitosis, genomic instability and cancer 10 Peter Walter Protein quality control l E.E. Just Lecture 11:00 am-12:00 pm Room 24B Supported by Howard Hughes Medical Institute
Erich D. Jarvis Duke University Medical Center/HHMI
A1 Dissecting the molecular mechanisms of ocalv learning and spoken language: a personal journey. E.D. Jarvis1; 1Department of Neurobiology, Duke University Medical Center & HHMI, Durham, NC
Organized by the ASCB Minorities Affairs Committee
l Odd-Numbered Poster Presentations 12:00-1:30 pm Learning Center
50 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l Microsymposium 1: Cell Motility and Migration 12:00-1:10 pm Microsymposia Room 1, Learning Center
Moderators: Pinar Gurel, National Heart, Blood, and Lung Institute, NIH; andBruno Da Rocha-Azevedo, Federal University of Rio de Janeiro; and Scott Wilkinson, Emory University
12:00 pm Introduction 12:05 pm E1 Cancer-associated fibroblasts promote directional migration of cancer cells via parallel organization of the fibronectin matrix. B. Erdogan1, M. Ao1, B.M. Brewer2, O.E. Franco3,4,5, S.W. Hayward3,4,5, D. Li2, D.J. Webb1,3; 1Biological Sciences, Vanderbilt University, Nashville, TN, 2Mechanical Engineering, Vanderbilt University, Nashville, TN, 3Cancer Biology, Vanderbilt University, Nashville, TN, 4Urologic Surgery, Vanderbilt University, Nashville, TN, 5Surgery, SUNDAY NorthShore University HealthSystem, Evanston, IL 12:10 pm E2 Centrosomes define the rear of migrating cells by modulating the distribution of inhibitory signals. J. Zhang1, Y. Wang1; 1Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 12:15 pm E3 A novel actin-adhesion structure requiring the formin FMN2 positions the nucleus and protects it from DNA damage during confined migration. C.T. Skau1, H. Racine Thiam1, G.M. Alushin1, P. Gurel1, A. Tubbs2, M. Piel3, A. Nussenzweig2, C.M. Waterman1; 1National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD,2 National Cancer Institute, National Institutes of Health, Bethesda, MD, 3Systems Cell Biology of Cell Polarity and Cell Division, Institut Curie, Paris, France 12:20 pm E4 Reconstitution of tumor microenvironment-associated high-speed breast cancer cell motility on aligned nanofibers. V.P. Sharma1,2, J. Williams3, E. Leung1, J. Sanders3, R.J. Eddy1, J. Castracane3, J.S. Condeelis1,2; 1Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY, 2Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, 3Colleges of Nanoscale Science and Engineering, SUNY Polytechnic Institute, Albany, NY 12:25 pm E5 CAMSAP2 and CAMSAP3 block trailing edge MT disassembly and nucleate leading edge MT growth during endothelial cell polarization and migration. P. Jones1, K.A. Myers1; 1Biological Sciences, University of the Sciences, Philadelphia, PA 12:30 pm E6 Leukocyte integrin LFA-1 is aligned and oriented by actin flow during cell migration. T.I. Moore1,2,3, P. Nordenfelt1,2,3,4, S. Mehta5, T. Lambert6, V. Swaminathan3,4,7, J.K. Mathew3,4,8, N. Koga9,10, D. Baker9,10, T. Tani5, S. Mayor3,4,8, C.M. Waterman3,4,7, T.A. Springer1,2,3; 1Program in Cellular and Molecular Medicine, Boston Childrens Hospital, Boston, MA, 2Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, 3Whitman Center, Marine Biological Laboratory, Woods Hole, MA, 4Physiology Course, Marine Biological Laboratory, Woods Hole, MA, 5Eugene Bell Center, Marine Biological Laboratory, Woods Hole, MA, 6Nikon Imaging Center, Harvard Medical School, Boston, MA, 7Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, NIH, Bethesda , MD,8 TIFR, National Centre for Biological Sciences, Bangalore, India, 9Biochemistry, University of Washington, Seattle, WA, 10Howard Hughes Medical Institute, Seattle, WA 12:35 pm E7 LSP-1 is a myosin-IIA binding regulator of podosome dynamics and macrophage migration. P. Cervero1, A. Bouiossou2, I. Maridonneau-Parini2, S. Linder1; 1Institute for Medical Microbiology, University Medical Center Eppendorf, Hamburg, Germany, 2CNRS UMR 5089, 2Institut de Pharmacologie et de Biologie Structurale , Toulouse, France
l Microsymposium 2: Signaling in Health and Disease 12:00-1:10 pm Microsymposia Room 2, Learning Center
Moderators: Paul Mungai, American Association for the Advancement of Science; and R. Ileng Kumaran, Cold Spring Harbor Laboratory 12:00 pm Introduction 12:05 pm E8 Spatial control of Shoc2 scaffold-mediated ERK1/2 signaling requires remodeling activity of the ATPase PSMC5. E. Galperin1, E. Jang1, H. JANG1, P. Shi1, G. Popa1, M. Jeoung1; 1Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 12:10 pm E9 Activation of the proteinase-activated receptor-2-β-arrestin-2 signaling axis by household allergens in the lung. M.C. Yee1, H.L. Nichols1, K. Pal1, D. Polley2, K.J. Lee1, M. Ming1, M.D.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 51 Seigler1, M.D. Hollenberg2, S. Boitano3, K.A. DeFea1; 1Division of Biomedical Sciences, University of California, Riverside, Riverside, CA, 2Department of Physiology & Pharmacology, University of Calgary, Calgary, Canada, 3Arizona Respiratory Center and Department of Physiology, University of Arizona, Tucson, AZ 12:15 pm E10 Optogenetic spatial control of TrkA-mediated pathways reveals a potential role for Raf/ERK pathway in inducing polarity in PC-12 cell differentiation model. Q. Ong1, K. Zhang2, A. McGuire1, S. Guo1, F. Santoro1, C. Zeng1, A.Y. Sarro-Schwartz1, R. Zhang1, B. Cui1; 1Department of Chemistry, Stanford University, Stanford, CA, 2Department of Biochemistry, University of Illinois at Urbana- Champaign, Urbana, IL 12:20 pm E11 Inhibition of one substrate phosphorylation of a protein kinase out of many substrates by a selective peptide inhibitor of kinase-substrate interaction. N. Qvit1, D. Mochly Rosen1, M. Disatnik1; 1Chemical and Systems Biology, Stanford, Stanford, CA 12:25 pm E12 The tetraspanin CD82 regulates hematopoietic stem cell fitness. C.A. Saito Reis1, K.D. Marjon1, K.L. Karlen1, R.J. Dodd1, C.M. Termini1, J.M. Gillette1; 1Pathology, University of New Mexico Health Science Center, Albuquerque, NM 12:30 pm E13 Pathological lymphangiogenesis is regulated by galectin-8-dependent crosstalk among VEGF-C, podoplanin and integrin pathways. W. Chen1, H. Leffler2, U.J. Nilsson3, L. Xia4, N. Panjwani1,5; 1Ophthalmology, Tufts University, Boston, MA,2 Microbiology Immunology and Glycobiology, Lund University, Lund, Sweden, 3Center for Analysis and Synthesis, Lund University, Lund, Sweden, 4Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 5New England Eye Center, Boston, MA 12:35 pm E14 Activation of HuR in a Gq-p38 MAPK-dependent manner promotes cardiac fibrosis and pathological remodeling. M. Tranter1, S.R. Anthony1, S. Slone1; 1Internal Medicine, Division of Cardiovascular Health and Disease, University of Cincinnati, Cincinnati, OH l Exhibitor Tech Talk 12:00-12:45 pm Theater 2, Learning Center
VitaCyte: Collagenase: moving from black box to transparent knowledge Presenter: Bob McCarthy Level: Introductory Crude collagenase is commonly used to recover cells from tissue. The benefit of using this low cost product comes at a price: the inability to define the biochemical components responsible for the success of cell recovery. As life science research transitions toward translational medicine, these reagents should be better defined. This tech talk provides an overview of how collagenase enzymes are manufactured, the limitations of using crude enzymes in cell isolation procedures, and the advantages of using a new defined, enriched collagenase product (DE Collagenase) that contains primarily collagenase and a purified protease. Comparison of the biochemical characteristics of crude and DE Collagenase enzymes will show how definition of key enzymes responsible for cell isolation minimizes the need to prequalify lots prior to purchase. l Exhibitor Tech Talk 12:00-12:45 pm Theater 1, Learning Center
EMD Millipore: Cell cycle and cell death: studying the connections with simplified cytometry Presenter: Kamala Tyagarajan, PhD Level: Introductory Understanding the linkages between cell cycle and cell death pathways has become increasingly important to elucidating the action of toxins and anti-cancer compounds, as well as the fundamental mechanisms of cell division. Cell biology research therefore requires simple, accessible tools to determine impacts on both cell cycle and cell health in order to characterize the pathways by which cells are affected by treatments or conditions. We will present simplified flow cytometric methods using the Muse© Cell Analyzer to study cell cycle distribution together with data from well-known apoptosis and cell death assays. You will learn how the parallel study of cell cycle and cell stress/death can provide enriched information on the impact of treatments, and provide a comprehensive picture of cellular status and health.
52 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l MD-PhD, Is It Right for Me? 12:00-12:55 pm Career Center Theater, Learning Center
Sandra Lemmon, PhD, University of Miami Miller School of Medicine, and Paul Insel, MD, University of California, San Diego This workshop will demystify the MD-PhD career and the application process to pursue an MD-PhD degree. The workshop will be presented by members of the MD-PhD Section of the Association of American Medical Colleges (AAMC) and will address general issues related to MD-PhD program training. Workshop topics include information on the careers of MD-PhD Physician-Scientists, how students train to become Physician-Scientists, how to apply to an MD-PhD training program, and what are the credentials of a competitive applicant. Panelists do not discuss individual programs, but present common properties of these programs. The workshop includes time for Q&A. Upon completion of the workshop, students, mentors, and advisors will have a working knowledge SUNDAY of MD-PhD careers, applying for the dual degree program, and how MD-PhD students are trained. Organized by the ASCB Committee for Postdocs and Students (COMPASS) l Table Talk 12:00-1:00 pm Roundtable Central Section 1, Learning Center
Foldscopes Manu Prakash, Stanford University Come learn about the Foldscope, a 50-cent print-and-fold mass-produced paper microscope from the Prakash lab. International scientist especially encouraged to attend.
l Afternoon Refreshment Break 12:00-4:00 pm Learning Center
Join us for iced tea and snacks while visiting exhibitors and viewing posters. Beer and wine are available for purchase from the consession stands.
l Table Talk 12:30-1:30 pm Roundtable Central Section 3, Learning Center
Lessons Learned about Starting a New Lab Omar Quintero, University of Richmond, and Paul Maddox, University of North Carolina Chapel Hill l Table Talk 12:30-1:30 pm Roundtable Central Section 2, Learning Center
National Research Mentoring Network Amy Prunuske, University of Minnesota l Exhibitor In-Booth Presentation 12:30 pm Booth 923
QImaging: You can have it all: new cameras, new advanced features, new software Presenter: Steven Smith, Product Manager
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 53 l Startups and Scientists: Our Strengths and Weaknesses as Entrepreneurs 1:00-1:55 pm Career Center Theater, Learning Center
A year ago, ASCB began to sponsor Startup Central in the Learning Center (Exhibit Hall) to feature more scientist-led startups. Expanding on this effort, this panel will be a moderated discussion by the founders of ASCB-exhibiting startups, covering topics around biomed/research entrepreneurship. Why do PhDs make good founders? What are the gaps in graduate training that scientist entrepreneurs find most challenging and surprising? Organized by the ASCB Committee for Postdocs and Students (COMPASS) l Exhibitor Tech Talk 1:00-2:45 pm Theater 1, Learning Center
1:00-1:45 pm: Carl Zeiss Microscopy, LLC: Accessing the emerging imaging technologies at HHMI Janelia Research Campus Presenter: Teng-Leong Chew, PhD, Director, Advanced Imaging Center, Howard Hughes Medical Institute Janelia Research Campus Level: Intermediate Visualizing and understanding complex biological processes demands the integrated efforts of biologists and physicists. The mission of the Advanced Imaging Center (AIC) is to make cutting-edge imaging technologies developed at Janelia widely accessible, and at no cost, to scientists, years before they become commercially available. This unique imaging center is thus uniquely positioned to empower investigators with tools currently not widely available elsewhere, such as the lattice light sheet microscope recently developed by Dr. Eric Betzig. In alignment with Janelia’s philosophy of encouraging bold and risky science, the AIC welcomes high-risk-high-gain projects that may challenge the current paradigm and provides full support through Janelia’s in-house imaging experts and research infrastructure. This seminar will present the technical capabilities and the application process of the AIC.
1:45-2:15 pm: Carl Zeiss Microscopy, LLC: New acquisition and detection modes with ZEISS Airyscan Presenter: Joseph Huff, Product Marketing Manager, Laser Scanning & Superresolution Microscopy Level: Intermediate Learn about two new detection and acquisition strategies for the ZEISS Airyscan detection module for laser scanning microscopy. ZEISS Airyscan, a new detector concept designed for improved laser scanning confocal microscopy, enables the simultaneous increase of both resolution and signal-to-noise ratio over traditional confocal imaging. Both modes—sensitivity and two-photon—extend the Airyscan benefits of resolution and signal-to-noise ratio to address more sample types.
2:15-2:45 pm: Carl Zeiss Microscopy, LLC: AiryScan: bringing super resolution to confocal microscopy Presenter: Dr. Xufeng Wu, NHLBI, NIH Level: Intermediate Confocal microcopy is and has been a mainstay of light microcopy for decades given the flexibility of a commercially available system. However, the resolution of a confocal microscope is still diffraction limited with a lateral resolution of ~250nm. With the recent advent of superresolution techniques (PALM, STORM, STED, and SIM), new commercially available systems are being used to image beyond the diffraction limit. As a result, the increased spatial resolution novel information regarding cell structure and function can be obtained. Recently the new Zeiss Airyscan detector was introduced as another method of superresolution and represents a good blend of resolution, sensitivity and speed. In this session, Dr. Xufeng Wu will present her recent experiences using the Airyscan for imaging cyoskeletal dynamics, organelle, membrane trafficking, and three-dimensional tissue architecture. l Exhibitor Tech Talk 1:00-1:45 pm Theater 2, Learning Center
Bruker Nano Surfaces: Advances in live super-resolution imaging using the Vutara 352 microscope Presenter: Manasa Gudheti, PhD Level: Intermediate Super-resolution microscopy has made a significant impact in the field of biological imaging. Most imaging has been targeted at fixed specimens with a few live-cell applications. The Vutara 352 super-resolution microscope has been engineered for live-cell applications through the optimization of spatial and temporal resolution in single-molecule localization imaging. Its sCMOS detector
54 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting enables video rate imaging along with two color simultaneous imaging in live cell and 3D particle tracking experiments. The biplane based detection path enables imaging thicker samples such as whole mountDrosophila and offers deeper penetration into tissues. The Vutara 352 also includes real time localization along with several statistical and live cell analysis features for processing data. In summary, the Vutara 352 microscope is a powerful super-resolution imaging and analysis tool. l Exhibitor In-Booth Presentation 1:00-2:00 pm Booth 1235/1237
National Institutes of Health (NIH): National Institute of General Medical Sciences (NIGMS) and Center for Scientific Review (CSR) Presenters: Zhongzhen Nie, Program Director, Division of Cell Biology and Biophysics, NIGMS, and David Balasundaram, Scientific Review Officer, Cell Biology Integrated Review Group, CSR SUNDAY R01 Application Strategy Discussion for New Principal Investigators
l Film: The Biology of Skin Color 1:00-1:20 pm ASCB Booth (721) Theater, Learning Center
Stop by to watch the new HHMI BioInteractive short film, The Biology of Skin Color. This 20-minute film, narrated by Dr. Nina Jablonski, presents a highly engaging case study in recent human evolution: the change in human skin color from the dark skin of our ancestors in equatorial Africa to the variety of skin colors that have evolved after human migration to other latitudes.
l Exhibitor In-Booth Presentation 1:15 pm Booth 934
Abcam, Inc: Immunostaining: From sample prep through troubleshooting and beyond Presenter: Thomas Novak, Abcam Scientific Support Specialist l Exhibitor In-Booth Presentation 1:15 pm Booth 921
Photometrics: Imaging with signal restoration super powers Presenter: Rachit Mohindra, Product Manager
l Microsymposium 3: Membrane Dynamics and Visualization 1:25-2:35 pm Microsymposia Room 1, Learning Center
Moderators: Theodore Ho, University of California, San Francisco; and Courtney Schroeder, University of California, San Francisco 1:25 pm Introduction 1:30 pm E15 Simultaneous observation of all endocytic events with single molecule sensitivity in a cell using lattice light-sheet imaging with high spatiotemporal resolution. S. Upadhyayula1, S. Arumugam2, R. Gaudin1, F. Aguet1, C. Wunder2, E. Betzig3, L. Johannes2, T. Kirchhausen1; 1Cell Biology, Harvard Medical School, Boston, MA, 2PSL Research University, Institut Curie, Paris, France,3 Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, VA 1:35 pm E16 Rapid exocytosis of an endolysosome-derived membrane domain forms a polarized invasive protrusion that clears basement membrane during cell invasion. K.M. Naegeli1, Q. Chi2, D.R. Sherwood2; 1Department of Pharmacology and Cancer Biology, Duke University, Durham, NC, 2Department of Biology, Duke University, Durham, NC 1:40 pm E17 Measuring GLUT4 vesicle exocytosis using intracellular intravital microscopy. A.J. Kee1, A. Masedunskas1, C.A. Lucas1, W. Han2, P.W. Gunning1, E.C. Hardeman1; 1School of Medical Sciences,
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 55 University of New South Wales, Sydney, Australia, 2Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Singapore 1:45 pm E18 High-resolution imaging of living cells by atomic force microscopy. A.L. Slade1, I. Medalsy1, S. Hu1, J.E. Shaw1, H. Schillers2; 1Bruker Nano Surfaces, Santa Barbara, CA, 2Institute of Physiology II, University of Muenster, Muenster, Germany 1:50 pm E19 ω-3 polyunsaturated fatty acids direct differentiation of the membrane phenotype in mesenchymal stem cells to potentiate osteogenesis. K.R. Levental1, M.A. Surma2, J.H. Lorent1, A. Skinkle3, C. Klose2, I. Levental1; 1Integrative Biology and Pharmacology, The University of Texas Health Science Center at Houston, Houston, TX, 2Lipotype, Dresden, Germany, 3Rice University, Houston, TX 1:55 pm E20 Nanoscale spatiotemporal organization of Fas receptor (CD95) during early stages of signaling revealed by quantitative superresolution microscopy. P. Sengupta1, A. Cruz2, R. Siegel2, J. Lippincott-Schwartz1; 1CBMP, NICHD, Bethesda, MD, 2Autoimmunity branch, NIAMS, Bethesda, MD 2:00 pm E21 A new ER structure revealed by live custom STED microscopy. L.K. Schroeder1, M. Deline2, J. Bewersdorf1, S. Bahmanyar2; 1Dept. of Cell Biology, Yale University, New Haven, CT, 2MCDB, Yale University, New Haven, CT l Microsymposium 4: Cell Division and Cytokinesis 1:25-2:35 pm Microsymposia Room 2, Learning Center
Moderators: Leocadia Paliulis, Bucknell University; and Patricia Wadsworth, University of Massachusetts 1:25 pm Introduction 1:30 pm E22 Symmetry and scale orient Min protein patterns in shaped bacterial sculptures. F. Wu1, C. Dekker1, J.E. Keymer1; 1Bionanoscience, Delft University of Technology, Delft, Netherlands 1:35 pm E23 Cdc42EP1 is a novel regulator of Septin organization during cytokinesis. A.L. Wilson1, S.J. Terry1, U.S. Eggert1; 1Randall Division of Cell and Molecular Biophysics, Kings College London, London, UK 1:40 pm E24 Understanding cellular variation in the molecular regulation of cytokinesis. T. Davies1, N. Romano Spica1, Y. Zhuravlev1, M. Shirasu-Hiza2, J. Dumont3, J.C. Canman1; 1Department of Pathology and Cell Biology, Columbia University, New York, NY, 2Department of Genetics and Development, Columbia University, New York, NY, 3CNRS, Institut Jacques Monod, Paris, France 1:45 pm E25 Force-dependent inhibition of formin Cdc12 by myosin Myo2 during in vitro reconstituted cytokinesis search, capture and pull. D. Zimmermann1, G.M. Hocky2, L.W. Pollard3, M.J. Lord3, D.R. Kovar1; 1Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL, 2Chemistry, The University of Chicago, Chicago, IL, 3Molecular Physiology and Biophysics, The University of Vermont, Burlington, VT 1:50 pm E26 Protein phosphatase 1 regulates ZYG-1 levels to limit centriole duplication. J. Iyer1, N. Peel2, A. Naik2, A.A. Hyman3, K.F. O’Connell1; 1NIDDK, National Institutes of Health, Bethesda, MD, 2Department of Biology, The College of New Jersey, Ewing, NJ, 3Molecular Biology and Genetics, Max Planck Institute of Molecular Biology and Genetics, Dresden, Germany 1:55 pm E27 Back-to-back mechanisms drive actomyosin ring contraction during Drosophila cellularization. Z. Xue1, A.M. Sokac1; 1Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 2:00 pm E28 A regulatory switch alters chromosome motions at the metaphase to anaphase transition. K. Su1, Z. Barry2, N. Schweizer3, A.J. Pereira3, H.J. Maiato3,4, M. Bathe2, I.M. Cheeseman1,5; 1Whitehead Institute, Cambridge, MA,2 Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 3Institute for Molecular and Cell Biology, University of Porto, Porto, Portugal, 4Department of Experimental Biology, Faculda de de Medicina, University of Porto, Porto, Portugal, 5Department of Biology, Massachusetts Institute of Technology, Cambridge, MA
56 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l Meet the Editor of CBE—Life Sciences Education 1:30-2:15 pm ASCB Booth (721), Learning Center
Erin Dolan SUNDAY University of Texas at Austin Editor-in-Chief
Stop by for an informal discussion about the journal with Editor-in-Chief Erin Dolan. Members of the CBE—Life Sciences Education editorial board will also be available at various times at the ASCB Journals Exhibit Booth (Booth 1226). Stop by the booth or check the Poster Guide for times. l Table Talk 1:30-2:30 pm Roundtable Central Section 2, Learning Center Incorporating Research into Lab Courses—Reports from REIL Biology Rachelle Spell, Emory University , Christopher Beck, Emory University, Pam Handson, Birmingham-Southern College, Mary Miller, Rhodes College l Table Talk 1:30-2:30 pm Roundtable Central Section 3, Learning Center
Using Cutting-Edge Models in the Classroom Megan Dobro, Hampshire College l Even-Numbered Poster Presentations 1:30 pm-3:00 pm Learning Center l Exhibitor In-Booth Presentation 2:00 pm Booth 320
Nanomedical Diagnostics: AGILE Research Biosensor live demonstration with complimentary beer Presenters: CTO Brett Goldsmith and VP Bio Francie Barron
l International Affairs Committee (IAC) Roundtable 2:00-3:00 pm Roundtable Central Section 1, Learning Center
By invitation only Moderator: Yixian Zheng, Carnegie Institution for Science
The goals of the IAC Roundtable are to foster interactions between U.S. and international graduate students and postdocs and discuss science and policy issues of special significance for international attendees. Members of the IAC, ASCB Council, and former ASCB Presidents facilitate discussions. Organized by the ASCB International Affairs Committee
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 57 l Exhibitor Tech Talk 2:00-2:45 pm Theater 2, Learning Center
Nikon Instruments, Inc.: Illuminating biology with super-resolution microscopy Presenter: Melike Lakadamyall, PhD Level: Intermediate Super-resolution microscopy has emerged as a powerful tool to image cells at the nanoscale, giving important insights into biological processes at the molecular level. The revolutionizing impact of these methods has recently been recognized by the Nobel Prize/ Chemistry. While exciting developments have significantly advanced the capabilities of super-resolution microscopy, important challenges remain in pursuing its applications in biology. One limitation is the compromise between spatial and temporal resolution that make these methods poorly suited to study dynamic processes. Another challenge is our limited capability to extract quantitative information from the super-resolution images, which is confounded by the photophysics of the fluorophores. I will show how we are developing new approaches to overcome these challenges and demonstrate novel biological applications of super-resolution.
l Career Panel: Science Policy 2:00-2:55 pm Career Center Theater, Learning Center These panelists will give a short talk about their career experiences and then will address individual questions from the audience. Paul Mungai, PhD, is an American Association for the Advancement of Science (AAAS), Science & Technology Policy Fellow, and the Science Officer in the Office of UNESCO Affairs at the U.S. Department of State. As Science Officer he is responsible for the Natural Sciences, and Social and Human Sciences portfolios, and acts as the primary liaison between the U.S. Mission to UNESCO in Paris and other science-related agencies and offices, primarily within the federal government. Mungai, along with partners at the National Park Service, has led the reinvigoration of a UNESCO ecology program focused on sustainable development and maintenance of biodiversity in the U.S. He also serves as the National Coordinator for the UNESCO Associated Schools Programme (ASPnet). Through the ASCB, Mungai helps develop programming to improve early scientist training and career development. As co-Chair of the ASCB Committee for Postdocs and Students (COMPASS) Outreach Subcommittee, he promotes science outreach that makes scientists and their research more accessible. Mungai is also a basic science researcher interested in how cells sense changes in cellular oxygen levels, and the protective mechanisms initiated when cells lack essential oxygen and nutrients. He received his doctorate in Cellular and Molecular Physiology from the University of Chicago in 2012, and was a postdoctoral fellow at the University of Illinois at Chicago. Yvette R. Seger, PhD, is the Director of Science Policy for the Federation of American Societies for Experimental Biology (FASEB), a coalition of 27 scientific societies collectively representing over 125,000 biological and biomedical researchers. In this role, she oversees FASEB’s science policy portfolio, and specifically manages efforts related to training and career opportunities for researchers and federal policies pertaining to grants management and peer review. Seger launched her policy career at the National Academies as a Christine Mirzayan Science & Technology Policy Fellow and subsequently held senior policy analyst positions at Thomson Reuters, the National Institutes of Health, and the research advocacy group FasterCures prior to joining FASEB. She holds a PhD in Genetics from Stony Brook University and received a BA in Zoology (Genetics Concentration) and Politics & Government from Ohio Wesleyan University. Organized by the ASCB Committee for Postdocs and Students (COMPASS) l Exhibitor In-Booth Presentation 2:00 pm Booth 923
QImaging: You can have it all: new cameras, new advanced features, new software Presenter: Steven Smith, Product Manager l Microsymposium 5: Mechanics in Cellular Maintenance and Disease 2:50-4:00 pm Microsymposia Room 1, Learning Center
Moderators: Alyssa Lesko and Cristian Suarez, The University of Chicago 2:50 pm Introduction 2:55 pm E29 Directed transport of a kinesin/cargo pair along newly polymerized microtubules into dendritic
58 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting spines undergoing synaptic plasticity. D.P. McVicker1, A.M. Awe1, K. Richters1, D.A. Cowdrey1, X. Hu1, E.R. Chapman1, E.W. Dent1; 1Neuroscience, University of Wisconsin-Madison, Madison, WI 3:00 pm E30 The role of N-cadherin signaling on endothelial barrier integrity. K.J. Kruse1, F. Huang1, Y. Sun1, S.M. Vogel1, Y.A. Komarova1, A.B. Malik1; 1Pharmacology, University of Illinois at Chicago, Chicago, IL 3:05 pm E31 Live multiplexed imaging of stem cell mechanotransduction and mechanoadaptation. I. Jalilian1, R. Oldfield1, P.W. Gunning2, M.L. Knothe Tate1; 1Graduate School of Biomedical Engineering, UNSW, Sydney, Australia, 2School of Medical Sciences, UNSW, Sydney, Australia 3:10 pm E32 Regulation of collateral branch formation in axons by MAP7-mediated microtubule bundle formation. L. Ma1, S. Tymanskyj1; 1Neuroscience, Thomas Jefferson University, Philadelphia, PA 3:15 pm E33 Controlling cell shape affects the spatial distribution of load across vinculin. K.E. Rothenberg1, S.S. Neibart1, A.S. LaCroix1, B.D. Hoffman1; 1Biomedical Engineering, Duke University, Durham , NC SUNDAY 3:20 pm E34 Formin-mediated cortex mechanics coordinate invasion by cell collectives. T. Fessenden1, Y. Beckham1, G.R. Ramirez-SanJuan1, M. Manning2, M.L. Gardel1,3,4; 1Institute for Biophysical Dynamics, University of Chicago, Chicago, IL, 2Physics, Syracuse University, Syracuse, NY, 3Physics, University of Chicago, Chicago, IL, 4James Franck Institute, University of Chicago, Chicago, IL 3:25 pm E35 Mena INV localizes to invadopodium precursors in breast carcinoma cells and dysregulates cortactin phosphorylation to promote matrix degradation by invadopodia. M.D. Weidmann1, V.P. Sharma1,2, R.J. Eddy1, J.S. Condeelis1,2; 1Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY, 2Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY l Microsymposium 6: Studying Organelle Function: New Trends and Technologies 2:50-4:00 pm Microsymposia Room 2, Learning Center
Moderators: Paulo Caceres and Anupam Das, Albany Medical College; and Gary McDowell, Tufts University 2:50 pm Introduction 2:55 pm E36 Quantitative multiplexed super-resolution neuronal synapse imaging using DNA-paint.S. Guo1,2, R. Veneziano2, S. Gordonov2, D. Park3, T. Kulesa2,4, P. Blainey2,4, E. Boyden2,3, M. Bathe2; 1Chemistry, MIT, Cambridge, MA, 2Biological Engineering, MIT, Cambridge, MA, 3Media Lab, MIT, Cambridge, MA, 4Broad Institute of Harvard and MIT, Cambridge, MA 3:00 pm E37 A novel video bioinformatics toolbox to study mitochondrial morphology, dynamics, and mitophagy in stressed stem cells. A. Zahedi1, R. Phandthong1, V. On2, P. Talbot1; 1Cell Biology & Neuroscience, University of California Riverside, Riverside, CA, 2Electrical Engineering, University of California Riverside, Riverside, CA 3:05 pm E38 Analyzing the spatial organization of synaptic molecules using SIM and object-based statistics.T. Lagache1, A. Grassart1, N. Sauvonnet1, O. Faklaris2, L.A. Danglot2, J. Olivo-Marin1; 1Cell Biology and Infection, Institut Pasteur, Paris, France,2 Institut Jacques Monod, Université Paris Diderot, Paris, France 3:10 pm E39 Global membrane geometry rather than membrane curvature underlies Min oscillations in Escherichia coli. J. Shen1,2,3, Y. Chang4, C. Chou1; 1Institute of Physics, Academia Sinica, Taipei, Taiwan, 2Department of Engineering and System Science, National Tsing Hua University, Hsinchu, Taiwan, 3Nano Science and Technology Program, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan, 4Department of Physics, National Taiwan Normal University, Taipei, Taiwan 3:15 pm E40 Protein disorder and protein-RNA interactions drive phase separation into liquid droplets with tunable viscoelasticity and dynamics. S. Elbaum-Garfinkle1, N. Vaidya1, N. Taylor1, C.P. Brangwynne1; 1Chemical & Bioengineering, Princeton University, Princeton, NJ 3:20 pm E41 Surface to volume relationships in the phenotype of vascular smooth muscle cells. R. Calizo1, P. Rangamani2, R. Iyengar1; 1Pharmacology and Systems Therapeutics, Icahn School of Medicine, New York, NY, 2Mechanical and Aerospace Engineering, University of California, San Diego, San Diego, CA 3:25 pm E42 Mutant KRAS-dependent Argonaute 2 (Ago2) sorting regulates miRNA secretion into exosomes. A.J. McKenzie1, D. Hoshino2, D.J. Cha3, J.G. Patton3, R.J. Coffey4, A.M. Weaver1,5,6; 1Cancer Biology, Vanderbilt University, Nashville, TN, 2Cancer Cell Research, Kanagawa Cancer Center, Yokohama, Japan, 3Biological Sciences, Vanderbilt University, Nashville, TN, 4Medicine, Vanderbilt University, Nashville, TN, 5Cell and Developmental Biology, Vanderbilt University, Nashville, TN, 6Pathology, Microbiology, and Immunology, Vanderbilt University, Nashville, TN
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 59 l Science Discussion Tables 3:00-3:50 pm Roundtable Central Section 3, Learning Center
Whether you’re a student, postdoc, or PI, ASCB will again offer special networking opportunities with senior scientists and peers. Select your interest area and bring your questions to the ASCB Learning Center in the San Diego Convention Center.
Table No. Presenter Topic 1 Lisa Belmont Why cell biology is important for drug discovery 2 Eric Betzig Microscopes for cell biology 3 Erich Jarvis Pursuing your scientific passions 4 Kathryn Lilley Organelles and spatial organization of the cell 5 Laura Machesky Cancer cell migration, invasion and metastasis 6 Tom Misteli Genome organization or career development 7 Susanne Rafelski Quantitative cell biology and QCBNet 8 Jody Rosenblatt Epithelial cell extrusion
l Exhibitor Tech Talk 3:00-4:00 pm Theater 2, Learning Center
Bitplane Inc.: Cell lineage analysis – Imaris 8.2 launch at ASCB 2015 Presenter: Luciano Lucas Level: Intermediate Low-toxicity imaging technologies have enabled an expanding group of researchers to examine cellular processes over long periods and at subcellular resolution. Developmental, stem cell, and cancer cell biology have all benefited greatly by employing techniques such as spinning disk confocal, light sheet, and structured illumination microscopy. A major bottleneck felt by scientists is the efficient visualization and analysis of long 3D time-lapses. Building on the powerful file handling of Imaris and functionality of ImarisTrack and ImarisCell, we are introducing a new tool set that enables researches to study cell division, track, and interactively explore cell lineages. This talk will be the launch of Imaris 8.2, which tackles the bottleneck mentioned above, thus enabling the scientific community to post and test previously impossible to test hypotheses. l WICB Network Reception Goes International 3:00-4:00 pm Room 33C
A panel of international cell biologists will discuss obstacles women face in their countries as they launch and develop their scientific careers. The panel will be followed by the annual WICB reception where you can meet, network, and enjoy refreshments. You will also learn more about two ASCB committees—Women in Cell Biology and International Affairs. Moderators: Sandra Masur, Chair, Women in Cell Biology Committee;Yixian Zheng, Chair, International Affairs Commitee Panelists: Ranan Aktas, Maltepe University (Turkey) Inke Nathke, University of Dundee (Scotland) Anne Spang, University of Basel (Switzerland) Xiaoyuan Song, University of Science and Technology of China Organized by the ASCB Women in Cell Biology and International Affairs Committees
60 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l Career Panel: Consulting and Entrepreneurship 3:00-4:00 pm Career Center Theater, Learning Center
These panelists will give a short talk about their career experiences and then will address individual questions from the audience.
Elmar Nurmemmedov, PhD, is the founder and CEO of BiotechLikemind. He earned his PhD in Molecular Biophysics from Lund University, Sweden. There, he spent four years studying structural and functional aspects of Wilms Tumor protein (WT1). During his postdoctoral training at Harvard Medical School, he followed his interest in transcription factors, this time from the drug discovery angle. Following that, he joined The Scripps Research Institute as a research associate, where he focused on discovery of small molecule inhibitors to beta-catenin, a cancer stem cell regulator. Throughout these years, Nurmemmedov has been interested in
the entrepreneurial aspects of science. He believes that entrepreneurship should be a viable career path for young scientists. He SUNDAY started BiotechLikemind when he was at Harvard and is currently expanding it. BiotechLikemind is an initiator of biotech, a social platform for development of early-stage ideas into products and startups. Melbs LeMieux, PhD, Co-Founder and Director of Materials and Process Engineering, C3Nano, is an expert on thin films and nanomaterials, with emphasis in polymeric, composite, textiles, and electronic materials. He is also active in technology commercialization and has a strong interest in enabling the development and realization of technologies and products incorporating advanced materials for the consumer electronics, biomedical, and wearables industries. His background includes direct experience in product and technology development in applications related to display and printed electronics. Since 2013, LeMieux has been working with the Enterprise Works Chicago start-up incubator, as an Entrepreneur in Residence. There, he works hands-on with students, postdocs, and faculty on understanding commercialization pathways for their technology. This involves technology due- diligence and commercialization road-mapping, market assessment, and intellectual property strategy with university, corporate, and private equity groups. Awarded an Intelligence Community Postdoctoral Fellowship, LeMieux spent three years at Stanford University’s Chemical Engineering Department, conducting research in the areas of organic flexible electronics. In 2010, he cofounded C3Nano, Inc. out of Stanford, developing printable inks composed of carbon nanotube, metal nanowire, and graphene materials for display, touchscreen, wearables, and electronic skin/biosensing applications. He has helped guide the company in winning the MIT Clean Energy Prize, as well as over $20M in fundraising. He received his PhD in materials science and engineering from Iowa State University, with emphasis on polymer physics and interfaces. He has co-authored over 40 publications and holds 10 patents. Elizabeth Tanner, PhD, is a biotechnology consultant focused on advancing the life sciences through technical and business focused solutions. She works closely with executive teams to create commercialization strategies and valuation models in the development and delivery of new biotechnology products. Tanner has 14 years of biomedical drug discovery laboratory experience in oncology, autoimmune disorders, and neurodegeneration. She received a BS in Biology from the University of Utah and a PhD in Molecular and Cellular Biology and Biochemistry from Boston University. She is currently an Adjunct Assistant Professor at University of San Diego, where she is working on her MBA. Organized by the ASCB Committee for Postdocs and Students (COMPASS) l HHMI Biointeractive Animations 3:00-4:00 pm ASCB Booth (721), Learning Center
These engaging, scientifically accurate animations cover a broad range of topics from the molecular machinery of a cell to speciation of anole lizards. Explore how we get our skin color, differentiation and cell fate, DNA replication, and more. Discuss how to use animations actively and effectively in the classroom. l Exhibitor In-Booth Presentation 3:00-4:00 pm Booth 335
3H Biomedical AB: Characterization and large scale expansion of human satellite cells Presenter: Mallen Huang
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 61 l Large-Scale Data Workshop: Computational Methods for RNA Sequencing Analysis 3:00-6:30 pm Room 31B
Manuel Garber Alper Kucukural University of Massachusetts Medical University of Massachusetts Medical School School The overview is open to all attendees. Preregistration is required for the hands-on portion of the workshop.
3:00-4:00 pm Didactic Overview. Open to all ASCB attendees. 4:00-6:30 pm Hands-on workshop. Limited to 50 preregistered participants. The workshop will focus on RNA-Seq application to gene expression. In the overview section we will describe the main computational components of gene expression analysis: 1) Alignment techniques and approaches and the impact of alignment on gene quantification; 2) Estimation of gene and isoform expression; and 3) Methods to compare samples with a focus on normalization and differential gene expression analysis. In the hands-on portion of the workshop we will rely on a previously published dataset to illustrate a full RNA-Seq analysis and the concepts discussed during the overview. We will guide participants through 1) Quality assessment of the data; 2) Alignment; 3) Transcript quantification using the RSEM software; 4) Differential gene expression analysis using DESeq; and 5) Typical analysis such as gene ontology, clustering, and principal component analysis using R. We expect all participants to have a laptop with wireless connection capability. Many activities will require a browser; only Firefox and Chrome have been tested. Some activities require issuing commands on a terminal. Participants running the Windows operating system must install putty, which can be downloaded from http://www.chiark.greenend. org.uk/~sgtatham/putty/download.html We encourage all participants to get familiar with R. We strongly suggest the R tutorial at http://tryr.codeschool.com/. If this session is full but people do not show up, we will let attendees in at the door on a first-come, first-served basis.
l Bruce Alberts Award for Excellence in Science Education 3:15-3:45 pm Room 24B
A2 Increasing Access for Underrepresented and Disadvantaged Students to Stem Related Professions
Deborah Harmon Hines University of Massachusetts Medical School Organized by the ASCB Education Committee
62 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l Multicellular Interactions, Tissues, and Development Minisymposium 1: Cell Migration in Tissues 4:00-6:25 pm Ballroom 20D
Co-Chairs: Alissa Weaver, Vanderbilt University Medical Center; and Celeste Nelson, Princeton University
4:00 pm Introduction 4:05 pm M1 Mechanical force dynamics during 3D collective migration. A.S. Piotrowski1, V.D. Varner1, C.M. Nelson1,2; 1Chemical Biological Engineering, Princeton University, Princeton, NJ, 2Molecular Biology, Princeton University, Princeton, NJ
4:25 pm M2 A novel image-guided genomics approach to dissect the mechanisms of collective cancer cell SUNDAY invasion. J.M. Konen1, A. Marcus2; 1Graduate Program in Cancer Biology, Emory University, Atlanta, GA, 2Hematology and Medical Oncology, Emory University, Atlanta, GA 4:45 pm M3 Twist1-induced epithelial dissemination is regulated by cell adhesion and heterotypic cell- cell dynamics. E.R. Shamir1, K. Sirka1, K. Coutinho1,2, M. Auer2, A.J. Ewald1; 1Cell Biology, Johns Hopkins School of Medicine, Baltimore, MD, 2Life Sciences, Lawrence Berkeley National Laboratory, Berkeley, CA 5:05 pm M4 PDGF signaling directs the medial movement of cardiomyocytes during the assembly of the heart tube. J. Bloomekatz1, A. Dunn1, M. Vaughan1, D. Yelon1; 1Division of Biology, University of California, San Diego, San Diego, CA 5:25 pm M5 Role of exosomes in promoting directional migration of cancer cells. B. Sung1, T. Ketova2, D. Hoshino3, A. Zijlstra1,2, A.M. Weaver1,2,4; 1Cancer Biology, Vanderbilt University School of Medicine, Nashville, TN, 2Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, 3Division of Cancer Cell Research, Kanagawa Cancer Center, Yokohama, Japan, 4Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 5:45 pm M6 Differentiation of the invasive phenotype requires G1 cell cycle arrest and HDAC-mediated regulation of gene expression. A.Q. Kohrman1, M. Chandhok1, W. Zhang1, D.Q. Matus1; 1Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 6:05 pm M7 Intravital imaging reveals ghost fibers as architectural units guiding muscle progenitors during skeletal muscle regeneration. M.T. Webster1, U. Manor2, J. Lippincott-Schwartz2, C. Fan1; 1Embryology, Carnegie Institution for Science, Baltimore, MD, 2NICHD, National Institutes of Health, Bethesda, MD 6:12 pm M8 Decoding embryonic developmental pathways using 4D-high content imaging of C. elegans embryos. R.A. Green1,2, S.D. Ochoa1,2, R. Khaliullin1,2, S. Wang1,2, Z. Zhao1,2, R.J. Biggs1,2, A. Gerson1,2, A.B. Desai1,2, K. Oegema1,2; 1Ludwig Cancer Research, San Diego, CA, 2Cellular and Molecular Medicine, University of California, San Diego, San Diego, CA 6:19 pm M9 Macrophage delivery service – a migrating source of extracellular matrix components is necessary for Drosophila embryogenesis. Y. Matsubayashi1, B.M. Stramer1; 1Randall Division of Cell and Molecular Biophysics, King’s College London, London, UK l Signaling and Differentiation Minisymposium 2: Cellular Decision-Making 4:00-6:25 pm Room 30C
Supported by BioMed Central Co-Chairs: Naama Barkai, Weizmann Institute of Science, Rehovot, Israel; and Jennifer Nemhauser, University of Washington
4:00 pm Introduction 4:05 pm M10 Combining whole cell modeling and optically reversible spatial mutations to dissect the 3D circuitry regulating the Caulobacter asymmetric developmental program. K. Lasker1, L. Shapiro1; 1Developmental Biology, Stanford University, Stanford, CA 4:25 pm M11 Role of the microtubule cytoskeleton in the control of Cdc42 GTPase and fission yeast cell shape emergence. M. Rodriguez1, F. Verde1; 1Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL 4:45 pm M12 A handoff model orf how asymmetric cell division triggers cell-specific gene expression in Bacillus
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 63 subtilis. N. Bradshaw1, R. Losick1; 1Molecular and Cellular Biology, Harvard University, Cambridge, MA 5:05 pm M13 Studying chemoattractant signal transduction dynamics in Dictyostelium by BRET. A.T. Islam1, P.G. Charest1; 1Chemistry and Biochemistry, University of Arizona, Tucson, AZ 5:25 pm M14 Kinome analysis in the giant ciliate Stentor coeruleus. S.B. Reiff1, P. Sood1, G. Ruby1, M. Slabodnick1, J. DeRisi1, W.F. Marshall1; 1Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 5:45 pm M15 Sensing and fusing: how fission easty use pheromone signaling to achieve fusion. O. Dudin1, S.G. Martin1; 1Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland 6:05 pm M16 From plants to yeast and back again: synthetic biology and plant development. J. Nemhauser1; 1Biology, University of Washington, Seattle, WA l Cell Cycle and Cell Division Minisymposium 3: Chromosome Segregation 4:00-6:25 pm Room 29C
Co-Chairs: Daniela Cimini, Virginia Tech; and Mary Dasso, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH
4:00 pm Introduction 4:05 pm M17 Aneuploidy confers a selective advantage to cancer cells by promoting karyotypic heterogeneity. S.D. Rutledge1,2, T.A. Douglas3, C.L. Kantzler1,2, D. Wangsa4, S.D. Kale2, E. Logarinho5, D. Cimini1,2; 1Biological Sciences, Virginia Tech, Blacksburg, VA, 2Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA,3 School of Biomedical Engineering and Sciences, Virginia Tech, Blacksburg, VA, 4Genetics Branch, NIH - National Cancer Institute, Bethesda, MD, 5Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal 4:25 pm M18 Quantitative assessment of chromosome instability induced through chemical disruption of mitotic progression. S. Markossian1, A. Arnaoutov1, N.S. Saba2, V. Larionov3, M. Dasso1; 1Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, MD, 2Section of Hematology and Medical Oncology, Department of Medicine, Tulane University, New Orleans, LA, 3Developmental Therapeutics Branch, National Cancer Institute, Bethesda, MD 4:45 pm M19 Aurora kinase phosphorylation of the Ndc80 tail antagonizes Ska complex-dependent lockdown of microtubule attachments. D.K. Cheerambathur1, K. Oegema1, A.B. Desai1; 1Dept. of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 5:05 pm M20 The Ska complex recruits Protein Phosphatase 1 to the kinetochore and promotes the metaphase- anaphase transition. S. Sivakumar1, P. Janczyk2, Q. Qu1, P. Stukenberg2, H. Yu1, G.J. Gorbsky3; 1Pharmacology, University of Texas-Southwestern, Dallas, TX, 2Biochemistry and Molecular Genetics, University of Virginia , Charlottesville, VA, 3Cell Cycle and Cancer Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 5:25 pm M21 The number of satellite repeats dictates centromere strength in mammals. A. Iwata-Otsubo1, S.J. Falk2, L. Chmatal1, M.A. Lampson1, B.E. Black3; 1Biology, University of Pennsylvania, Philadelphia, PA, 2Graduate Group in Cell and Molecular Biology, University of Pennsylvania, Philadelphia, PA, 3Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 5:45 pm M22 Centromere maintenance through error correction of CENP-A deposition during DNA replication. Y. Nechemia-Arbely1, K.H. Miga2, M. McMahon1, D. Fachinetti1, A. Lee1, B. Ren1, D.W. Cleveland1; 1Cellular and Molecular Medicine, Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA, 2Center for Biomolecular Science Engineering, University of California Santa Cruz, Santa Cruz, CA 6:05 pm M23 Mitotic synthesis and processing of centromere-derived RNAs promotes kinetochore and spindle assembly in Xenopus. A.W. Grenfell1, M. Strzelecka1, R. Heald1; 1Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 6:12 pm M24 Purification of the chromosome passenger complex from mitotic chromosomes reveals functions in centromere transcription and cohesion. L. Liu1, M.C. Barnhart1, E. Zasadzinska1, C.A. Kestner1, J. Yates III2, D. Daniel Foltz 1, P. Stukenberg1; 1Biochemistry and Molecular Genetics, University of Virginia, School of Medicine, Charlottesville, VA, 2Chemical Physiology, The Scripps Research Institute, La Jolla, CA 6:19 pm M25 The genome proximity sensor: a novel failsafe that triggers p53 accumulation in aneuploid cells
64 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting in response to chromosome missegregation. C. Day1, Z. Dong1, K.T. Vaughan2, E.H. Hinchcliffe1; 1Hormel Institute, University of Minnesota, Austin, MN, 2Biological Sciences, University of Notre Dame, Notre Dame, IN l Cell Biology of Genetic Information Minisymposium 4: Genome Organization and Stability 4:00-6:25 pm Room 28D
Co-Chairs: Sarah Elgin, Washington University; Sui Huang, Northwestern University Feinberg School of Medicine; and Steven Kosak, Northwestern University Feinberg School of Medicine SUNDAY 4:00 pm Introduction 4:05 pm M26 Early replication stress leads to abnormal mitosis and genome rearrangement. S.A. Sabatinos1,2, N.S. Ranatunga1, J. Yuan1, M. Green1, S.L. Forsburg1; 1Molecular & Computational Biology, University of Southern California, Los Angeles, CA, 2Chemistry & Biology, Ryerson University , Toronto, ON 4:25 pm M27 DNA damage and chromothripsis from chromosome segregation errors. A. Spektor1,2,3, Z. Cheng-Zhong2,3,4,5, N.T. Umbreit2,3, H. Cornils2,3, J.M. Francis4,5, E.K. Jackson2,3,6, S. Liu2,3, M.L. Meyerson4,5,7,8, D.S. Pellman2,3,5,6; 1Radiation Oncology, Dana-Farber Cancer Institute, Boston, MA, 2Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA, 3Cell Biology, Harvard Medical School, Boston, MA, 4Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, 5Broad Institute of Harvard and MIT, Boston, MA, 6Howard Hughes Medical Institute, Chevy Chase, MD, 7Center for Cancer Genome Discovery, Dana-Farber Cancer Institute, Boston, MA, 8Pathology, Harvard Medical School, Boston, MA 4:45 pm M28 A chemical proteomics approach reveals direct binders of DNA-damage-associated histone variant gammaH2AX. R.E. Kleiner1, P. Verma1, K.R. Molloy2, B.T. Chait2, T.M. Kapoor1; 1Laboratory of Chemistry and Cell Biology, Rockefeller University, New York, NY, 2Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, Rockefeller University, New York, NY 5:05 pm M29 Dynamic phosphoregulation of axis proteins underlies chromosome remodeling during meiosis. Y. Kim1,2,3, S.C. Rosenberg4,5, N. Kostow1,2,3, O. Rog1,2,3, S. Köhler1,2,3, K.D. Corbett4,5, A.F. Dernburg1,2,3,6; 1Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 2California Institute for Quantitative Biosciences, Berkeley, CA, 3Howard Hughes Medical Institute, Chevy Chase, MD,4 San Diego Branch, Ludwig Institute for Cancer Research, La Jolla, CA, 5Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA, 6Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 5:25 pm M30 Pol I transcription and nucleolar structure play key roles in nuclear organization. C. Wang1, S.B. Sondalle2, S.J. Baserga2, S. Huang1; 1Cell and Molecular Biology, Northwestern University, Feinberg School of Medicine, Chicago, IL, 2Department of Genetics, Yale University School of Medicine, New Haven, CT 5:45 pm M31 “TSA-Seq” reveals 3D organization of the human genome. Y. Chen1, Y. Zhang2, L. Zhang1, E. Brinkman3, Y. Wang4, B. van Steensel3, J. Ma2,4, A.S. Belmont1,4; 1Cell and Developmental Biology, University of Illinois, Urbana-Champaign, Urbana, IL, 2Bioengineering, University of Illinois, Urbana-Champaign, Urbana, IL, 3Division of Gene Regulation, Netherlands Cancer Institute, Amsterdam, Netherlands, 4Program in Biophysics and Computational Biology, University of Illinois, Urbana-Champaign, Urbana, IL 6:05 pm M32 The human genome is dynamically polarized during epidermal differentiation. A.M. Wood1, B. Poll1, S.T. Kosak1; 1Cell & Molecular Biology, Northwestern University, Feinberg School of Medicine, Chicago, IL
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 65 l Membrane Organization, Dynamics, Traffic, and Regulation Minisymposium 5: Mechanisms for Shaping Membranes 4:00-6:25 pm Ballroom 20A
Co-Chairs: Karen Davies, Max Planck Institute of Biophysics, Frankfurt, Germany; Marko Kaksonen, University of Geneva, Switzerland and European Molecular Biology Laboratory, Heidelberg
4:00 pm Introduction 4:05 pm M33 Determining the molecular basis of cristae structure by electron cryo-tomography. K.M. Davies1,2, A.W. Mühleip1,2, T. Blum1, B. Daum1, C. Anselmi3, J. Faraldo-Gomez3, W. Kühlbrandt1,2; 1Structural Biology, Max Planck Institute of Biophysics, Frankfurt am Main, Germany, 2Cluster of Excellence ‘Macromolecular Complexes’, Goethe University, Frankfurt am main, Germany, 3Theoretical Molecular Biophysics Section, National Heart, Blood and Lung Institute, Bethesda, MD 4:25 pm M34 *Ultrafast endocytosis of synaptic vesicles. S. Watanabe1, T. Trimbuch2, M. Camacho-Perez2, B. Rost2, C. Rosenmund2, E. Jorgensen1; 1Department of Biology, University of Utah, Salt Lake City, UT, 2Neurocure, Charite Universitatsmedizin, Berlin, Germany 4:45 pm M35 Superresolution imaging of clathrin-mediated endocytosis in yeast. M. Mund1, A. Picco1,2, M. Kaksonen1,2, J. Ries1; 1Cell Biology and Biophysics, EMBL, Heidelberg, Germany, 2Biochemistry, University of Geneva, Geneva, Switzerland 5:05 pm M36 Direct probing of dynamic phosphoinositide switches during clathrin-mediated endocytosis. K. He1,2, E. Song1,2, M. Ma1,2, R. Gaudin1,2, T. Kirchhausen1,2; 1Department of Cell Biology, Harvard Medical School, Boston, MA, 2Program in Cellular and Molecular Medicine, Boston Childrens Hospital, Boston, MA 5:25 pm M37 Membrane curvature regulates the biogenesis of COPII coated transport carriers. M. Hanna1, I. Mela2, J. Edwardson2, A. Audhya1; 1Biomolecular Chemistry, University of Wisconsin-Madison, Madison, WI, 2Department of Pharmacology, University of Cambridge, Cambridge, UK 5:45 pm M38 Intrinsically disordered proteins drive membrane curvature and modulate the cargo content of coated vesicles. D.J. Busch1, J.R. Houser1, C.C. Hayden1, M.B. Sherman2, E.M. Lafer3, J.C. Stachowiak1,4; 1Department of Biomedical Engineering, University of Texas at Austin, Austin, TX, 2Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, 3Department of Biochemistry and Center for Biomedical Neuroscience, University of Texas Health Science Center at San Antonio, San Antonio, TX, 4Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 6:05 pm M39 Subcellular recruitment of clathrin-mediated endocytosis machinery in genome-edited cells to sites of nanostructure-induced membrane curvature. W. Zhao1, L.A. Hanson2, P. Chowdary2, J.R. Marks3, S. Hong3, D.G. Drubin3, Y. Cui1,4, B. Cui2; 1Department of Materials Science and Engineering, Stanford University, Stanford , CA, 2Department of Chemistry, Stanford University, Stanford , CA, 3Department of Molecular & Cell Biology, University of California, Berkeley, Berkeley, CA, 4Stanford Institute for Materials and Energy Sciences, SLAC National Accelerator Laboratory, Menlo Park, CA 6:12 pm M40 Autoregulation and membrane composition coordinate the membrane remodeling and actin assembly activities of the F-BAR/SH3 protein Nervous Wreck. C.F. Kelley1, E. Messelaar1, T. Stanishneva-Konovolova2, T.L. Eskin1, S.A. Wasserman1, O. Sokolova2, A.A. Rodal1; 1Molecular and Cell Biology, Brandeis University, Waltham, MA, 2Bioengineering, Moscow State University, Moscow, Russia 6:19 pm M41 Regulation of the ESCRT-III membrane scission machine by a ubiquitin hydrolase. N.K. Johnson1, M. West1, G. Odorizzi1; 1Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO
*Shigeki Watanabe is the recipient of the Merton Bernfield Memorial Award
66 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l Cytoskeleton, Motility, and Cell Mechanics Minisymposium 6: Molecular Motors and the Cytoskeleton: Measurement, Manipulation, and Mechanics 4:00-6:25 pm Ballroom 20C
Co-Chairs: Zev Bryant, Stanford University; and Samara Reck-Peterson, University of California, San Diego
4:00 pm Introduction 4:05 pm M42 Engineering cytoskeletal motors. Z. Bryant1,2; 1Bioengineering, Stanford University, Stanford, CA, 2Structural Biology, Stanford University School of Medicine, Stanford, CA 4:25 pm M43 Force generation by membrane associated myosin-IC. S. Pyrpassopoulos1, G. Arpağ2, E. Feeser1, H. Shuman1, E. Tuzel2, E.M. Ostap1; 1The Pennsylvania Muscle Institute and Department of SUNDAY Physiology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, 2Department of Physics, Worcester Polytechnic Institute, Worcester, MA 4:45 pm M44 Shape remodelling and blebbing of active cytoskeletal vesicles. E. Loiseau1, J.A. Schneider2, F.C. Keber1, G. Salbreux2, A.R. Bausch1; 1Physics Departement, Technische Universität München, Munich, Germany, 2Lincoln’s Inn Fields Laboratories, The Francis Crick Institute, London, UK 5:05 pm M45 On the force-generating capacity of disassembling microtubules. C.L. Asbury1, J.W. Driver1, E. Geyer2, L.M. Rice2; 1Physiology & Biophysics, University of Washington, Seattle, WA, 2Biophysics, University of Texas Southwestern, Dallas, TX 5:25 pm M46 Microtubules self-repair in response to mechanical stress. L. Schaedel1, K. John2, J. Gaillard1, M.V. Nachury3, L. Blanchoin1, M. Théry1,4; 1iRTSV, CEA, Grenoble, France, 2Laboratoire Interdisciplinaire de Physique, CNRS, Grenoble, France, 3Department of Molecular and Cellular Physiology, Stanford University School of Medicine, San Francisco, CA, 4IUH, INSERM, Paris, France 5:45 pm M47 Developing a method for mathematical computation of three-dimensional EB1-GFP motion visualized by lattice light-sheet microscopy. N. Yamashita1, M. Morita1, W.R. Legant2, B. Chen2,3, E. Betzig2, H. Yokota1, Y. Mimori-Kiyosue4; 1Image Processing Research Team, RIKEN Center for Advanced Photonics, RIKEN, Wako, Saitama, Japan, 2Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA,3 Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan, 4Cellular Dynamics Analysis Unit, RIKEN Center for Life Science Technologies, RIKEN, Kobe, Hyogo, Japan 6:05 pm M48 Direct measurement of the binding rate constant of kinesin to microtubules in living cells. T. Kambara1, Y. Okada1; 1Quantitative Biology Center, Riken, Suita, Japan 6:12 pm M49 O-Myo! A ring-shaped myosin gliding assay for characterizing the lifetime of myosin motors. R.F. Hariadi1, A. Appukutty2, S. Sivaramakrishnan3; 1Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 2Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, 3Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 6:19 pm M50 Structural insights into cytokinesis: super-resolution imaging of cytokinesis nodes and contractile rings in live fission yeast. C. Laplante1, F. Huang2, I.R. Tebbs1, J. Bewersdorf2,3, T.D. Pollard1,2,4; 1MCDB, Yale University, New Haven, CT, 2Cell Biology, Yale University, New Haven, CT, 3Biomedical Engineering, Yale University, New Haven, CT, 4Molecular Biophysics and Biochemistry, Yale University, New Haven, CT l Education Minisymposium: Teaching How to Teach and Learn 4:00-6:25 pm Room 32B
Co-Chairs: Jennifer Hood-DeGrenier, Worcester State University; and Tony Koleske, Yale University Supported by CBE—Life Sciences Education
4:00 pm Introduction 4:05 pm M51 The development and validation of tools to help biology departments navigate from Vision to Change. S.E. Brownell1, S. Freeman2, M. Wenderoth2, A. Crowe2; 1School of Life Sciences, Arizona State University, Tempe, AZ, 2Biology, University of Washington, Seattle, WA 4:25 pm M52 Converting a lecture-based introductory biology class to an active learning studio environment. J.N. Schoonmaker1, C.J. Ramey1; 1Chemical and Biological Engineering, Colorado School of Mines, Golden, CO
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 67 4:45 pm M53 Teaching computational approaches for life scientists. A. Rubinstein1; 1Computer Science, Tel Aviv University, Tel Aviv, Israel 5:05 pm M54 MACH: a model for explaining molecular and cellular mechanisms across the life science disciplines. C.M. Trujillo1,2, T. Anderson3, N. Pelaez2; 1Plant Biology, Michigan State University, East Lansing, MI, 2Biological Sciences, Purdue University, West Lafayette, IN,3 Chemistry, Purdue University, West Lafayette, IN 5:25 pm M55 Barriers to engagement with the primary literature and the journey from a novice to a competent reader. C. Abdullah1, R. Lie2, E. Tour3; 1Biomedical Sciences Program, UCSD, La Jolla, CA, 2Department of Neurosciences, UCSD, La Jolla, CA, 3Cell and Developmental Biology, UCSD, La Jolla, CA 5:45 pm M56 Spreading Vision and Change through faculty mentorship: The ASCB Mentoring in Active Learning and Teaching (MALT) program. M.J. Wolyniak1, A.J. Prunuske2, J.J. Adler3, A.J. Crowe4, L.C. Keller5, B.J. Kolber6, B.A. Leland7, S. Murugesan8, S.M. Schreiner7, Z. Whatley9, S.M. Wick10; 1Biology, Hampden-Sydney College, Hampden-Sydney, VA, 2Biomedical Sciences, University of Minnesota Medical School, Duluth, MN, 3Biology, Brescia University, Owensboro, KY, 4Biology, University of Washington, Seattle, WA,5 Biological Sciences, Quinnipiac University, Hamden, CT, 6Biological Sciences, Duquesne University, Pittsburgh, PA,7 Cell Biology, Yale University School of Medicine, New Haven, CT, 8Cell Biology and Physiology, National Heart, Lung, and Blood Institute, Bethesda, MD, 9Biology, Gettysburg College, Gettysburg, PA,10 Plant Biology, University of Minnesota, St. Paul, MN 5:55 pm M57 Developing an understanding of scientific research helps prepare STEM undergraduates to teach. E.M. Stone1, A. Baranger2; 1Berkeley Science & Math Initiative, University of California, Berkeley, Berkeley, CA, 2Department of Chemistry, University of California, Berkeley, Berkeley, CA 6:05 pm M58 Stop telling me to do active learning and show me how: biology examples of active learning techniques. K. McCoy-Simandle1, L.B. Jones2, D. Cox1; 1Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY, 2Biological Sciences, Lehman College, Bronx, NY 6:15 pm M59 StarCellBio: a new cell and molecular biology experiment simulator. A.L. Brauneis1, L.M. Aleman1,2, I. Ceraj2, S. Kini2, W. Lisa1, A. Gavrilman2, P. Pinch2, C.A. Kaiser1, G. Walker1; 1Biology, Massachusetts Institute of Technology, Cambridge, MA,2 Office of Digital Learning, Massachusetts Institute of Technology, Cambridge, MA l Exhibitor Tech Talk 4:15-5:15 pm Theater 2, Learning Center
Horizon Discovery: Genome editing in human cells using CRISPR/Cas technology Presenter: Dr. Daniella Steel Level: Intermediate Genome engineering has been revolutionized recently by the emergence of CRISPR-Cas9, which has democratized the generation of custom engineered cell lines. Unfortunately, as many users are discovering, the reality isn’t always as simple as hoped. Horizon is at the forefront of gene editing and has amassed the world’s largest collection of off-the-shelf or on-demand genomically modified cell lines (>20,000 X-MAN® models) available to support research programs worldwide. We will cover CRISPR-Cas9 as a tool for cell line generation, discuss how to plan a CRISPR genome editing experiment to maximize your chances of success, and focus on the applications of these cell lines, exploring through case studies the genomic modifications available to cell biologists, including knockouts, knockins, translocations, and endogenous tagging.
68 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l ASCB Kaluza Prize Presentation and Keith R. Porter Lecture 6:45 pm-8:00 pm Ballroom 20BC
ASCB Kaluza Prizes Supported by Beckman Coulter SUNDAY
Guangbo Chen, Uri Ben-David, Pavithra Aravamudha, for work done at Stowers Broad Institute of MIT and for work done at Institute for Medical Research Harvard, for work done University of Michigan at Hebrew University of Jerusalem Cash prizes of $5,000, $3,000, and $1,000 will be presented to the top three of 10 finalists for excellence in graduate student research.
Seven others were named winners of the ASCB Beckman Coulter Distinguished Graduate Student Achievement Prize: Lindsay Case, University of Texas Southwestern Medical School, for work done at NIH Lukas Chmatal, for work done at the University of Pennsylvania Phillip Dumesic, for work done at the University of California, San Francisco Laura Gaydos, Fred Hutchinson Research Center, for work done at University of California, Santa Cruz Ryan Flynn, for work done at Stanford University Kailin Mesa, for work done at Yale University Graham Walmsley, for work done at Stanford University School of Medicine
Keith R. Porter Lecture
Jonathan S. Weissman University of California, San Francisco/HHMI
A3 Monitoring translation in space and time with ribosome profiling.J.S. Weissman1,2; 1Cellular & Molecular Pharmacology, University of California San Francisco, San Francisco, CA, 2Howard Hughes Medical Institute, Chevy Chase, MD
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 69 Notes
70 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Monday December 14, 2015
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 71 7:30 am-6:00 pm Registration Open Registration Area 7:30 am-8:00 pm Career Center Open Learning Center 8:00 am-9:30 am Symposium 3 Ballroom 20BC Embraces across the Species Barrier: Complex Cell Interactions 8:15-9:15 am Exhibitor Tech Talk Theater 1, Learning Center Leica Microsystems: Infinite possibilities in live cell imaging: advances in confocal and widefield imaging by Leica Microsystems 8:15-9:15 am Exhibitor Tech Talk Theater 2, Learning Center TESCAN USA, INC.: Digital holographic microscopy: a revolutionary approach for visualizing cellular morphology in real time 9:00-10:00 am Green Cards for Scientific Researchers: U.S. Immigration Options Career Center Theater, Learning Center 9:30-4:30 pm ASCB Learning Center (Exhibit Hall) Open 9:30-11:30 am Morning Refreshment Break Learning Center 9:30-10:00 am Table Talk Roundtable Central Section 3, PALM Network Learning Center
9:30-10:30 am Exhibitor Tech Talk Theater 2, Learning Center Bruker Nano Surfaces: Opterra II: 4D confocal imaging at the speed of life 9:30-10:30 am Exhibitor Tech Talk Theater 1, Learning Center GE Healthcare: Advances in design for increased specificity and function in CRISPR/CAS9 and shRNA based eneg modulation 9:45-10:45 am Symposium 4: Like Oil and Water: New Principles Ballroom 20BC Governing Cell Organization 10:00-11:00 am COMPASS Open Forum Room 33C 10:00-11:00 am Simple Social Media for Scientists Career Center Theater, Learning Center
10:30 am-12:00 pm Advocacy Toolbox: The Two-Minute Speech Room 33B 10:45 am-12:00 pm Career Discussion and Mentoring Roundtables Roundtable Central Sections 1-3, Learning Center
10:45-11:45 am Exhibitor Tech Talk Theater 1, Learning Center Wiley: Open Access in publishing 10:45-11:45 am Exhibitor Tech Talk Theater 2, Learning Center Etaluma, Inc.: Enabling low cost drug discovery with genetically encoded biosensors and “next generation” imaging Daily Schedule—Monday, December 14 11:00 am-12:00 pm Answers to Questions about Peer Review Career Center Theater, Learning Center
11:00 am-12:00 pm Fast-Growing Cell Biology Society in China Room 23B 12:00-1:30 pm Odd-Numbered Poster Presentations Learning Center 12:00-1:10 pm Cytoskeleton, Motility, and Cell Mechanics Microsymposia Room 1, Microsymposium 7: Microtubule Dynamics: Learning Center From +TIPs to Membrane 12:00 pm-1:10 pm Multicellular Interactions, Tissues, and Development Microsymposia Room 2, Microsymposium 8: The Role of the Cytoskeleton in Learning Center Disease and Repair
12:00 pm-12:55 pm Programmatic Efforts at NIH to Promote and Support the Career Center Theater, Careers of Women in Biomedical Science Learning Center 12:00-12:45 pm Exhibitor Tech Talk Theater 1, Learning Center GE Healthcare: Live cell structured illumination imaging: a new reality 12:00-4:00 pm Afternoon Refreshment Break Learning Center 12:15 pm Exhibitor In-Booth Presentation Booth 934 Abcam, Inc: Immunostaining: From sample prep through troubleshooting and beyond 12:30-1:30 pm Table Talk Roundtable Central Section 3, Council on Undergraduate Research Learning Center
72 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 12:30 pm Exhibitor In Booth Presentation Booth 320 Nanomedical Diagnostics: AGILE Research Biosensor live demonstration with complimentary beer 1:00-2:00 pm MALT Workshop Roundtable Central Section 1, Reforming Undergraduate STEM Education through Learning Center Long-Term Mentorships: The ASCB Mentoring in Active Learning and Teaching (MALT) Program 1:00-1:45 pm Exhibitor Tech Talk Theater 2, Learning Center ACEA Biosciences: Use of real time cellular analysis and flow cytometry to characterize potential biomarkers of aggressive colorectal cancer 1:00-1:45 pm Exhibitor Tech Talk Theater 1, Learning Center Thermo Fisher Scientific: Engineering fluorescence for cell biology 1:00-1:55 pm Career Options and Job Resources for Scientists Career Center Theater, Learning Center Daily Schedule—Monday, December 14 1:00-2:00 pm Exhibitor In-Booth Presentation Booth 1235/1237 National Institutes of Health (NIH): National Institute of General Medical Sciences (NIGMS) and Center for Scientific Review (CSR) 1:15 pm Exhibitor In-Booth Presentation Booth 921 Photometrics: Imaging with signal restoration super powers 1:25-2:35 pm Membrane Organization, Dynamics, Traffic, and Regulation Microsymposia Room 1, Microsymposium 9: Membrane Trafficking Learning Center 1:25-2:35 pm Applications of Cell Biology in the Real World Microsymposia Room 2, Microsymposium 10: Applications of Cell Biology Learning Center in the Real World 1:30-2:15 pm Meet the Editor of Molecular Biology of the Cell ASCB Booth (721), Learning Center 1:30-3:00 pm Even-Numbered Poster Presentations Learning Center 2:00-3:00 pm Table Talk Roundtable Central Section 2, Teaching and Research at a Small, Liberal Arts College Learning Center 2:00-2:45 pm Exhibitor Tech Talk Theater 1, Learning Center Cellecta Inc.: CRISPR and RNAi: gene-editing and functional genomic screening approaches 2:00 pm Exhibitor In-Booth Presentation Booth 923 QImaging: You can have it all: new cameras, new advanced features, new software 2:00-2:45 pm Exhibitor Tech Talk Theater 2, Learning Center EMD Millipore: Winning Westerns for cell biologists: get reliable expression data from lysates 2:00-2:55 pm Career Panel: Science Communication Career Center Theater, Learning Center 2:45 pm Exhibitor In-Booth Presentation Booth 934 Abcam, Inc: Immunostaining: From sample prep through troubleshooting and beyond 2:50-4:00 pm Organelles and Spatial Organization of the Cell Microsymposia Room 1, Microsymposium 11: Nucleus Biology and Disease Learning Center 2:50-4:00 pm Signaling and Differentiation Microsymposia Room 2, Microsymposium 12: Signaling in Differentiation and Cancer Learning Center 3:00-3:50 pm Science Discussion Tables Roundtable Central Section 3, Learning Center
3:00-3:55 pm Career Panel: Teaching and University Administration Career Center Theater, Learning Center
3:00-4:00 pm Exhibitor Tech Talk Theater 1, Learning Center ALVÉOLE: Multi-protein printing by light-induced molecular adsorption: application to cell biology 3:00-4:00 pm 3Is in South Korea: Initiatives, Innovations, and Information for Bioscience Room 23B
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 73 3:00-4:00 pm Exhibitor In-Booth Presentation Booth 1235/1237 National Institutes of Health (NIH): National Institute of General Medical Sciences (NIGMS) and Center for Scientific Review (CSR) 3:00-4:00 pm Exhibitor In-Booth Presentation Booth 335 3H Biomedical AB: Characterization and large scale expansion of human satellite cells 3:00-4:00 pm Exhibitor Tech Talk Theater 2, Learning Center BioTek Instruments: Kinetic live cell imaging 3:00-6:30 pm Large-Scale Data Workshop: Quantitative Analysis and Visualization of Room 31B Signaling Networks 3:15-3:45 pm Education Initiative Forum Room 24B Integrating Discovery-Based Research into the Undergraduate Curriculum: Report on an NAS Convocation 4:00-6:00 pm Networking Happy Hour Career Center, Learning Center 4:00-6:25 pm Kaluza Minisymposium Room 32B
Cell Cycle and Cell Division Room 28D Minisymposium 7: Centrosomes and Spindles
Membrane Organization, Dynamics, Traffic, and Regulation Ballroom 20A Minisymposium 8: Lipid Organization, Transport, Composition, and Phosphoinositides
Cytoskeleton, Motility, and Cell Mechanics Ballroom 20C Minisymposium 9: Microtubule-Based Motility and Dynamics
Signaling and Differentiation Room 30C Minisymposium 10: New Technologies and Immuno-Signaling
Cell Biology of Genetic Information Room 29C Minisymposium 11: Nuclear Mechanics and Transport
Organelles and Spatial Organization of the Cell Ballroom 20D Minisymposium 12: Organelle Dynamics, Structure, and Function
4:15-5:15 pm Exhibitor Tech Talk Theater 2, Learning Center Applied Scientific Instrumentation Inc.: Advances in dual inverted selective plane microscopy (diSPIM) and laser technology 4:15-5:15 pm Exhibitor Tech Talk Theater 1, Learning Center Daily Schedule—Monday, December 14 NanoSurface Biomedical, Inc.: Nano-engineering cell phenotype and function with aligned nanopatterned cell culture dishes Montana Molecular: Single fluorescent protein-based biosensors for detecting Gi, Gs, and Gq-coupled pathways of GPCR signaling VitaScientific: New tools for cell biology from VitaScientific.com 5:30-7:45 pm Exhibitor Tech Talk Theater 2, Learning Center Nanolive SA: Product Launch: 3D Cell Explorer, revolutionary microscope able to image living cells instantly, in 3D and 4D 6:45-8:00 pm Emerging Topic Symposium - Mitochondria and Cancer Cell Biology Ballroom 20D
74 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Monday, December 14 l Career Center 7:30 am-8:00 pm Learning Center
Stop by any time to check out the job postings. 9:00 am–4:00 pm One-on-One CV Review Drop in and have an experienced ASCB member help you hone the perfect CV. Personalized Career Coaching Drop by to talk one-on-one with life sciences career experts about your personal career trajectory. Immigration Consultation Stop by for a free individual immigration consultation from experts from Getson & Schatz.
l Symposium 3: Embraces across the Species Barrier: Complex Cell Interactions 8:00-9:30 am Ballroom 20BC MONDAY
Supported by The American Association of Anatomists and The Anatomical Record Chair: Kerry Bloom, University of North Carolina at Chapel Hill
8:00 am S6 Wolbachia, microtubules and Big Sur. W.T. Sullivan1, L.R. Serbus2, P. White1; 1MCD Biology, University of California, Santa Cruz, Santa Cruz , CA, 2Biology, Florida International University, Miami, FL 8:30 am S7 Interactions across biological scales within the cheese rind ecosystem. R.J. Dutton1; 1Division of Biological Sciences, Section of Molecular Biology, University of California, San Diego, La Jolla, CA 9:00 am S8 Bacteriophage adherence to mucus (BAM) immunity: how phage and the microbiome form innate and acquired immune systems on mucosal surfaces. F. Rohwer1; 1Biology, San Diego State University, San Diego, CA l Exhibitor Tech Talk 8:15-9:15 am Theater 1, Learning Center
Leica Microsystems: Infinite possibilities in live cell imaging: advances in confocal and widefield imaging by Leica Microsystems Presenter: TBD Level: Intermediate Innovation in advanced imaging is a driving force for science. Leica Microsystems’ newest inverted microscope platform, the Leica DMi8, features modularity that allows users to build a powerful imaging system specific to your research and budget today without compromising essential functionality for the future. Examples of the platform’s modularity include FRAP, confocal imaging, digital light sheet, and super-resolution STED and GSD. Powered by its modularity, unique Infinity Port™ and next generation 19mm FOV imaging ports, the DMi8 allows users to see more of their data. l Exhibitor Tech Talk 8:15-9:15 am Theater 2, Learning Center
TESCAN USA, INC.: Digital holographic microscopy: a revolutionary approach for visualizing cellular morphology in real time Presenter: Vratislav Kostal, PhD Level: Intermediate
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 75 Digital holographic microscopy (DHM) is an emerging microscopic technique for high resolution, label-free observations of living cells. Compared with standard microscopy, DHM has the unique ability to record complete information about the light waves including intensity, DIC contrast and especially the phase shift. The quantitative phase imaging (QPI) provides unique insight into the distribution of cellular mass. Here, we present the latest version of our QPI microscope (Tescan QPHASE), which is designed specifically for long-term studies of cellular dynamics and morphology. A unique illumination setup allows imaging of cellular mass and morphology with extreme axial sensitivity and no halo effects. The unique capabilities of the system are demonstrated by time lapse studies of cellular death, cancer cell proliferation, and cell behavior in 3D environments. l Green Cards for Scientific Researchers: U.S. Immigration Options 9:00-10:00 am Career Center Theater, Learning Center
Learn how to maximize your chances of qualifying for an EB-1/NIW green card from leading U.S. immigration lawyer, Brian H. Getson, Esq., author of “Obtaining a US Visa Based on Achievement: What You Need to Know” and a graduate of the University of Pennsylvania School of Law with 20 years of experience. The Getson Immigration Law Group is a 2015 ASCB Gold Sponsor and exhibitor. l ASCB Learning Center (Exhibit Hall) Open 7:30 am-8:00 pm Exhibits open 9:30 am-4:00 pm. l Morning Refreshment Break 9:30-11:30 am Learning Center
Join us for complimentary coffee and tea while visiting exhibitors and viewing posters.
l Table Talk 9:30-10:00 am Roundtable Central Section 3, Learning Center
PALM Network Sue Wick, University of Minnesota The NSF-funded Promoting Active Learning & Mentoring (PALM) Network aims to improve undergraduate biology education in line with the goals of Vision and Change. Our focus is promoting evidence-based active learning in classrooms, especially reaching individuals underrepresented in biology. Interested in being a PALM Fellow or mentor? l Exhibitor Tech Talk 9:30-10:30 am Theater 2, Learning Center
Bruker Nano Surfaces: Opterra II: 4D confocal imaging at the speed of life Presenter: Jimmy Fong Level: Intermediate The Opterra imaging system is a high speed confocal system designed for live cell, volumetric microscopy. The Opterra II builds upon the success of the first generation Opterra’s Swept Field Confocal (SFC) technology by offering rapid filter switching, quality wide- field/DIC imaging via bypass mode, enhanced optics for field uniformity, and sophisticated photo-stimulation capability. Flexibility in choosing various pinhole and slit apertures has allowed researchers to balance spatial resolution, speed, and fluorescence intensity for each experiment. Come learn about the variety of research enabled by the instrument including studies of wound-healing processes in the Xenopus laevis, neural development in zebrafish, tracking of multi-labeled cilia, and others.
76 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l Exhibitor Tech Talk 9:30-10:30 am Theater 1, Learning Center
GE Healthcare: Advances in design for increased specificity and function in CRISPR/CAS9 and shRNA based gene modulation Presenter: James Goldmeyer, PhD, Global Product Manager Level: Intermediate The emergence of the CRISPR-Cas9 system to generate alterations in the mammalian genome has expanded the scope of functional biology and many parallels have been drawn between the CRISPR-Cas9 system and the RNAi pathway. While fundamentally different, the challenges of off-targeting and poor functionality can be inherent to both systems and cause concerns about data interpretation and reliability. Used together, these technologies can provide a robust insight into the functions of genes in mammalian cells. In this talk we will describe a novel approach to understanding the requirements of both function and specificity of crRNAs and present data supporting the development of the next generation of shRNA constructs.
l Symposium 4: Like Oil and Water: New Principles Governing Cell Organization 9:45-10:45 am Ballroom 20BC
Chair: David Drubin, University of California, Berkeley 9:45 am S9 Physical mechanisms of cell organization on micron length scales. M.K. Rosen1; 1Biophysics, UT MONDAY Southwestern Medical Center/HHMI, Dallas, TX 10:15 am S10 Phase separation in cytoplasm: implications for polarity and neurodegeneration. A.A. Hyman1; 1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
l COMPASS Open Forum 10:00-11:00 am Room 33C
Graduate students and postdocs: Learn how you can become involved in shaping the ASCB through the COMmittee for Postdocs and StudentS (COMPASS). The session will begin with a 15-minute overview of COMPASS followed by an informal discussion with COMPASS representatives. Stop by to chat one-on-one with members of the Career Development, Communications, Social, and Outreach subcommittees. Attendees are welcome to drop in at any time. Organized by the ASCB Committee for Postdocs and Students (COMPASS) l Simple Social Media for Scientists 10:00-11:00 am Career Center Theater, Learning Center
Mónica I. Feliú-Mójer, PhD, Program Manager, iBiology
Social media can be an invaluable tool to increase the impact and visibility of scientific research. For example, papers shared on social media have increased citations and downloads. Social media offers new ways to expand your professional network; connect with policymakers and the public; communicate scientific research; and advance your professional career. This session will provide 1) an introduction to social media platforms, particularly Twitter; 2) tangible examples of how social media use can benefit your research and career; and 3) resources and strategies to use social media effectively. Organized by the ASCB Committee for Postdocs and Students (COMPASS)
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 77 l Advocacy Toolbox: The Two-Minute Speech 10:30 am-12:00 pm Room 33B
Simon Atkinson Sue Biggins Daniel Fletcher Holly Goodson Kathleen J. Green Indiana University Fred Hutchinson University of University of Notre Northwestern Purdue University Cancer Research Center California, Berkeley Dame University Indianapolis
Dan Kiehart Anthony J. Koleske Connie Lee Thomas D. Pollard Mark Winey Duke University Yale University The University of Yale University University of Chicago Colorado, Boulder
If you were in an elevator with President Obama, how would you explain to him what you do? How do you explain your work to the chatty guy sitting next to you on an airplane? If you’re not sure or think you need to improve your explanation, you need to come to this session and improve your own two-minute speech with help from experienced science policy advocates. Organized by the ASCB Public Policy Committee
l Career Discussion and Mentoring Roundtables 10:45 am-12:00 pm Roundtable Central Sections 1-3, Learning Center
(No preregistration required; first-come, first served) Supported by a grant from The Burroughs Wellcome Fund Coordinator: Debra Page Baluch, Arizona State University The Career Discussion and Mentoring Roundtables allow participants to meet informally for discussions on issues of importance to cell biologists in various stages of their careers. Conversations are moderated by individuals who have experience in various professional areas or with particular issues and also benefit from discussion around the table. The session is an excellent way to disseminate practical information on career choices, to discuss strategies for effectively developing a career, and to network with others who share career interests and concerns. Attending these roundtables can help overcome the intimidating aspects of the large Annual Meeting, especially for young cell biologists for whom it is critical to find mentors among cell biologists as they progress in their careers. Past attendees say that meeting others with common interests and concerns at this event enriched their initial contacts and provided positive feedback and excellent advice regarding a career issue of concern to them.
78 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Table Topics Career Options C1 Careers in Biotech, Pharmaceutical & Industry P3 Applying for a PUI Faculty Position C2 Careers in Patent Law, Intellectual Property P4 Setting Up Your First Laboratory C3 Careers in Scientific Writing & Editing P5 Developing Research Teams & Collaborations C4 Careers in Computational Biology, Bioinformatics P6 Leader and Management Skills C5 Careers as Research Scientists/Academic Professionals P7 Teaching and Research in PUIs C6 Extended Postdocs P8 Funding Opportunities for Research at PUIs C7 Research in Government Labs P9 Funding Opportunities for Academic Research C8 Strategies for Obtaining a Postdoc P10 Teaching Tools & Strategies C9 International Postdocs: In the U.S. and Abroad Career and Life C10 Undergraduate Research and Career Opportunities L1 Work/Life Satisfaction Career Preparation L2 Women in Science P1 Interviewing & Negotiation Skill Development L3 LGBTQ in Science P2 Applying for an Academic Faculty Position
Organized by the ASCB Women in Cell Biology Committee MONDAY
l Exhibitor Tech Talk 10:45-11:45 am Theater 1, Learning Center
Wiley: Open Access in publishing Presenter: Andrew Moore, Editor-in-Chief Level: Introductory-Intermediate Attend this Open Access workshop with Andrew Moore, a highly-experienced Wiley Editor. Andrew will be talking about Open Access; the changes it’s brought to the field, what you need to know, and what it means for you. Come and improve your understanding of Open Access and take the opportunity to ask Andrew any questions you have on the topic.
l Exhibitor Tech Talk 10:45-11:45 am Theater 2, Learning Center
Etaluma, Inc.: Enabling low cost drug discovery with genetically encoded biosensors and “next generation” imaging Presenters: Alex Zambon and Chris Shumate Level: Intermediate If a picture is worth a thousand words, then a movie should be worth tens of thousands of data points. The timely integration of affordable time-lapse live-cell microscopy provided by Etaluma, along with genetically encoded biosensors and quantitative image analysis approaches, have enabled our laboratory to develop a robust and extremely low cost screen. Our genetically encoded biosensor harnesses cell-cycle-dependent transcriptional and post-transcriptional regulation of fluorescent reporter proteins that ultimately enable multi-parameter quantification of each of the main phases of the cell cycle (i.e., G0, G1, S, G2/M) at single cell resolution in living cells and tissue. This economical system is being applied to novel drug and gene discovery in regenerative medicine and cancer.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 79 l Answers to Questions about Peer Review 11:00 am-12:00 pm Career Center Theater, Learning Center
Keith A. Mintzer, Scientific Review Officer, National Heart, Lung, and Blood Institute, NIH Do you have questions about NIH peer review? Come get answers from an NIH staffer who organizes peer review meetings. Attendees will watch and discuss a video of a mock NIH peer review meeting. There will be ample time to ask questions about peer review and other aspects of the NIH grants process.
l Fast-Growing Cell Biology Society in China 11:00 am-12:00 pm Room 23B
Presenters: Jiahuai Han, Dangsheng Li, Xueliang Zhu, Guangshuo Ou, and Xuebiao Yao, Chinese Society of Cell Biology This session will highlight the fast-evolving Chinese Society for Cell Biology, two fast-growing state key laboratories for cell biology, and prominent journals of cell biology research publishing in China. It will provide an interactive forum to explore potential opportunities in international study, scientific collaboration, and career development. The event is specifically designed for those who plan to explore graduate and postdoc training, academic employment opportunities and/or establish collaborative efforts in China. Organized by the ASCB International Affairs Committee l Odd-Numbered Poster Presentations 12:00-1:30 pm Learning Center
l Cytoskeleton, Motility, and Cell Mechanics Microsymposium 7: Microtubule Dynamics: From +TIPs to Membrane 12:00-1:10 pm Microsymposia Room 1, Learning Center
Moderators: Pinar Gurel, National Heart, Blood, and Lung Institute, NIH; andAlyssa Lesko, University of Notre Dame
12:00 pm Introduction 12:05 pm E43 Active contraction of microtubule networks. P.J. Foster1,2, S. Fürthauer3,4, M.J. Shelley4, D.J. Needleman1,2,3; 1School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 2FAS Center For Systems Biology, Harvard University, Cambridge, MA, 3Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, 4Courant Institute of Mathematical Science, New York University, New York, NY 12:10 pm E44 The kinesin-4 KIF21B is specialized to independently regulate trafficking and microtubule dynamics in dendrites. A.E. Ghiretti1, E.L. Holzbaur1; 1Department of Physiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 12:15 pm E45 Cytoskeletal processes in vitro in 3D: new perspective on the motor tug-of-war. M. Vershinin1, O. Osunbayo1, J. Bergman1; 1Physics and Astronomy, University of Utah, Salt Lake City, UT 12:20 pm E46 Kinesin-5 is a microtubule polymerase. W.O. Hancock1, Y. Chen1; 1Biomedical Engineering, Penn State University, University Park, PA 12:25 pm E47 Direction specific microtubules are the rails for interflagellar transport trains. G. Pigino1, L. Stepanek1; 1Molecular Cell Biology and Genetics, Max Planck Institute, Dresden, Germany 12:30 pm E48 Spatiotemporal control of intracellular microtubule dynamics by light. J. Van Haren1, A.W. Ettinger1, H. Wang2, K.M. Hahn2, T. Wittmann1; 1Cell and Tissue Biology, University of California, San Francisco, CA, 2Pharmacology, University of North Carolina, Chapel Hill, NC 12:35 pm E49 A complex relationship between motor activity, cytoplasmic flows and the organisation of the actin and microtubule cytoskeletons. I.M. Palacios1, M. Drechsler1, S. Ganguly2; 1Zoology, University of Cambridge, Cambridge, UK, 2Yale University, New Haven, CT
80 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l Multicellular Interactions, Tissues, and Development Microsymposium 8: The Role of the Cytoskeleton in Disease and Repair 12:00 pm-1:10 pm Microsymposia Room 2, Learning Center
Moderators: Alana Gray, Louisiana State University Health Sciences Center, Shreveport; and Gary McDowell, Tufts University; and Swaran Nandini, University of Central Florida
12:00 pm Introduction 12:05 pm E50 Dynamic force patterns promote collective cell migration and rapid wound repair. T. Zulueta- Coarasa1, R. Fernandez-Gonzalez1,2,3; 1Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, 2Department of Cell and Systems Biology, University of Toronto, Toronto, ON, 3Developmental and Stem Cell Biology Program, The Hospital for Sick Children, Toronto, ON 12:10 pm E51 Abnormal cortical neuron migration by perturbed nucleus-centrosome coupling underlies the pathophysiology of autism with abnormality in RBFOX1/A2BP1 gene. K. Nagata1, N. Hamada1, H. Ito1, I. Iwamoto1, R. Morishita1, H. Tabata1; 1Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, Kasugai, Japan 12:15 pm E52 The physical interaction between tumor and endothelial cells is a key inducer for liver cancer angiogenesis in a HepG2-HUVEC co-culture system. G. Chiew1, A. Fu1, K.Q. Luo1; 1School of
Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore MONDAY 12:20 pm E53 Abnormal expression of laminin-332 promotes cell proliferation and cyst growth in autosomal recessive polycystic kidney disease. S. Vijayakumar1, S. Dang2, D.P. Wallace3, B.K. Yoder4, P. Marinkovich5, Z. Lazarova6, V.E. Torres7; 1Natural Sciences & Mathematics Department, SUNY Cobleskill, Cobleskill, NY, 2Department of Medicine, University of New England College of Osteopathic Medicine, Biddeford,, ME, 3Department of Medicine, University of Kansas Medical Center, Kansas City, KS, 4Department of Cell Biology, University of Alabama, Birmingham, AL, 5Program in Epithelial Biology, Stanford University School of Medicine, Stanford, CA, 6Department of Dermatology, Medical College of Wisconsin, Milwaukee, WI, 7Division of Nephrology & Hypertension, Mayo Clinic, Rochester, MN 12:25 pm E54 Sentinel cells of the social amoeba Dictyostelium traps and kills bacteria by casting DNA nets. X. Zhang1, O. Zhuchenko2, K. Adam2, T. Soldati1; 1Department of Biochemistry, University of Geneva, Geneva, Switzerland, 2Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 12:30 pm E55 Modeling polycystic kidney disease cystogenesis with genome-modified human pluripotent stem cells. B.S. Freedman1,2, T.I. Steinman3, J. Zhou2, J.V. Bonventre2; 1Department of Medicine, Division of Nephrology, University of Washington, Seattle, WA, 2Department of Medicine, Renal Division, Brigham and Women’s Hospital/Harvard Medical School, Boston, MA, 3Department of Medicine, Division of Nephrology, Beth Israel Deaconess Medical Center/Harvard Medical School, Boston, MA 12:35 pm E56 Macrophage-dependent activation of Notch1 signaling regulates breast tumor cell intravasation. J. Pignatelli1, J. Bravo Cordero1,2,3, M. Roh1, S. Gandhi1, Y. Wang1, R.H. Singer1,2, L. Hodgson1,2, M. Oktay1,4, J.S. Condeelis1,2; 1Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY, 2Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronc, NY, 3Medicine, Mount Sinai , New York, NY, 4Pathology, Albert Einstein College of Medicine, Bronx, NY
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 81 l Programmatic Efforts at NIH to Promote and Support the Careers of Women in Biomedical Science 12:00-12:55 pm Career Center Theater, Learning Center
Jennifer Plank-Bazinet, PhD, Health Scientist Administrator, NIH Office of Research on Women’s Health While women have reached parity at the training level in the biomedical workforce, they are still significantly underrepresented in the professoriate and mid- and senior-level life science positions. Considerable effort has been devoted by individuals and organizations across science sectors to understanding this disparity and to developing interventions in support of women’s career development. The National Institutes of Health (NIH) formed the Office of Research on Women’s Health (ORWH) in 1990 with the goal of supporting initiatives to improve women’s health, while also including the task of providing opportunities and support for the recruitment, retention, reentry, and advancement of women in biomedical careers. Plank-Bazinet will review several accomplishments and flagship activities initiated by NIH and ORWH in support of women’s career development. While the programs that will be discussed are preferentially used by women, they are also readily available and beneficial to men. Plank- Bazinet will discuss the unique opportunities that accompany NIH partnerships with the scientific community, and conclude with recommendations for future research and programmatic support for women in the biomedical workforce. Organized by the ASCB Committee for Postdocs and Students (COMPASS) l Exhibitor Tech Talk 12:00-12:45 pm Theater 1, Learning Center
GE Healthcare: Live cell structured illumination imaging: a new reality Presenter: Leanna Ferrand, Product Support Leader Level: Intermediate Live cell imaging has long been one of the most challenging yet most rewarding applications in fluorescence microscopy. Recently, super-resolution microscopy has allowed researchers to investigate previously unresolvable structural details in their samples. Combining these techniques to perform live cell super-resolution imaging creates additional challenges, but greatly increases the potential scientific reward. Advances in Structured Illumination Microscopy (SIM) have made biologically relevant live cell SIM a reality. SIM offers researchers a two-fold increase in resolution both laterally and axially, revealing those structural details previously unresolved with conventional microscopy. SIM requires the least amount of input light, thus reducing photobleaching and phototoxicity. SIM is compatible with standard fluorophores and sample preparation techniques, requiring minimal sample optimization and is becoming the most approachable live cell super-resolution method. l Afternoon Refreshment Break 12:00-4:00 pm Learning Center
Join us for ice tea and snacks while visiting exhibitors and viewing posters. Beer and wine will be available for purchase at the concession stands. l Exhibitor In-Booth Presentation 12:15 pm Booth 934
Abcam, Inc: Immunostaining: From sample prep through troubleshooting and beyond Presenter: Thomas Novak, Abcam Scientific Support Specialist l Table Talk 12:30-1:30 pm Roundtable Central Section 3, Learning Center
Council on Undergraduate Research Joyce Fernandes, Miami University of Ohio, and Lance Barton, Austin College The Council on Undergraduate Research: A professional society that supports and promotes student-faculty collaborative research and scholarship.
82 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l Exhibitor In Booth Presentation 12:30 pm Booth 320
Nanomedical Diagnostics: AGILE Research Biosensor live demonstration with complimentary beer Presenters: CTO Brett Goldsmith and VP Bio Francie Barron l MALT Workshop 1:00-2:00 pm Roundtable Central Section 1, Learning Center
Reforming Undergraduate STEM Education through Long-Term Mentorships: The ASCB Mentoring in Active Learning and Teaching (MALT) Program
A pedagogical movement is under way in undergraduate biology education to put active learning techniques and inquiry- based learning at center stage as proven methods to excite and retain students. As part of this movement, instructors need accessible and sustainable ways to learn how to integrate active learning into their teaching. The Mentoring in Active Learning and Teaching (MALT) program, an initiative of the ASCB Education Committee, seeks to pair active learning experts with instructors, postdoctoral fellows, and graduate students who wish to gain experience in teaching via active learning. MALT mentorships are not one-time workshops but rather long-term partnerships that allow the mentee to practice and adapt active learning techniques into his or her teaching. At this workshop, current MALT participants will discuss how they have effectively established active learning-based MONDAY mentorships, what changes they have made in their classrooms, and how they have assessed the results of their work. We will also discuss how the program may best evolve to fit the needs of the ASCB membership and welcome members who are interested in becoming involved in the program. Organized by the ASCB Education Committee
l Exhibitor Tech Talk 1:00-1:45 pm Theater 2, Learning Center
ACEA Biosciences: Use of real time cellular analysis and flow cytometry to characterize potential biomarkers of aggressive colorectal cancer Presenter: Steve Offer, Assistant Professor of Pharmacology, Mayo Clinic School of Medicine Level: Advanced Steve Offer will describe the cellular metastasis models his lab has developed to better understand the molecular changes that occur during colorectal cancer metastasis. He will discuss the correlation between changes in the epigenetic landscape, microRNA expression, and gene expression, and how these changes affect tumor progression. Along with traditional approaches to study metastasis, Offer’s lab has leveraged the throughput and sensitivity of the xCELLigence real-time cell analyzer and the NovoCyte flow cytometer to test specific parameters of candidate metastasis-associated signatures identified by his lab. These results are expected to help identify early-stage indicators of later tumor aggressiveness and to facilitate the development of therapeutics designed to specifically target cancers based on discrete molecular profiles. l Exhibitor Tech Talk 1:00-1:45 pm Theater 1, Learning Center
Thermo Fisher Scientific: Engineering fluorescence for cell biology Presenter: Nick Dolman, PhD, Sr. Staff Scientist, R&D Level: Intermediate The combination of light microscopy and fluorescent reporters offers an unparalleled view into the function of intact cells. Recently, the scientific community has witnessed many major innovations in fluorescence microscopy that have paved the way for exciting new discoveries. Invitrogen™ Molecular Probes™ reagents have represented a significant aspect of these key innovations, driving cutting edge fluorescent reporter development for four decades. In this seminar, we will highlight the breadth and depth of fluorescent
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 83 probes available to the cell biologist of today, drawing on specific techniques as diverse as flow cytometry , single molecule and in vivo imaging to high content, and multicolor time lapse imaging. Specific examples of assays to visualize key aspects of cell biology will also be described that include cytoskeletal dynamics, cell tracing, endocytosis, proliferation, autophagy, hypoxia, oxidative stress, and apoptosis. Furthermore, we will provide an overview of new probes and current constraints in serving emerging areas such as 3-D cellular models and super resolution microscopy. l Career Options and Job Resources for Scientists 1:00-1:55 pm Career Center Theater, Learning Center
Tianna Hicklin, Assistant Editor, Science/AAAS Custom Publishing Planning a successful and rewarding scientific career means considering all your career options and discovering what is exciting to you. Learning how your scientific training fits with many different career possibilities and developing new and transferable skills are important for creating your individualized career path. In this seminar, we’ll discuss different career options, how to find the best fit for your personality, and developing a career plan. Science Careers is the careers component of Science that scientists rely on for career information and job postings.Science Careers offers many free resources for scientists to learn about their options as well as career development advice. Visit www.sciencecareers. org for more information. Organized by the ASCB Committee for Postdocs and Students (COMPASS) l Exhibitor In-Booth Presentation 1:00-2:00 pm Booth 1235/1237
National Institutes of Health (NIH): National Institute of General Medical Sciences (NIGMS) and Center for Scientific Review (CSR) Presenters: Alexandra Ainsztein, Program Director, Division of Cell Biology and Biophysics, NIGMS; and Janet Larkin, Scientific Review Officer, Cell Biology Integrated Review Group, CSR NIH AREA Grants l Exhibitor In-Booth Presentation 1:15 pm Booth 921
Photometrics: Imaging with signal restoration super powers Presenter: Rachit Mohindra, Product Manager l Membrane Organization, Dynamics, Traffic, and Regulation Microsymposium 9: Membrane Trafficking 1:25-2:35 pm Microsymposia Room 1, Learning Center
Moderators: Brooke Gardner, University of California, Berkeley; and Kellyann Jones-Jamtgaard, University of Kansas Medical Center 1:25 pm Introduction 1:30 pm E57 Human Sar1 paralogs differ biochemically in the assembly of the COPII coat. D.B. Melville1,2, S. Studer1,2, R.W. Schekman1,2; 1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 2Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 1:35 pm E58 Calcyon forms a complex with cytoplasmic dynein and regulates cargoes transport in mature neurons. L. Shi1, N. Muthusamy2, Z. Roth1, C. Bergson3, D. Smith1; 1Department of Biological Sciences, University of South Carolina, Columbia, SC, 2Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, NC, 3Department of Pharmacology and Toxicology, Georgia Regents University, Augusta, GA 1:40 pm E59 Peroxisomes move by hitchhiking on early endosomes using the novel adaptor protein PxdA. J. Salogiannis1, S.L. Reck-Peterson1,2; 1Cell Biology, Harvard Medical School, Boston, MA, 2Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA
84 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 1:45 pm E60 Akt stabilizes a Rab11-WDR44 interaction to regulate pre-ciliary vesicle trafficking and ciliogenesis initiation. V. Walia1, C. Insinna1, Q. Lu1, S. Specht1, Z. Meng1, M. Zhou1, D. Ritt1, D. Morrison1, C.J. Westlake1; 1National Cancer Institute, Frederick, MD 1:50 pm E61 A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region. C.M. Schroeder1, J.M. Ostrem1, N.T. Hertz2, R.D. Vale1; 1Cellular and Molecular Pharmacology , University of California, San Francisco, San Francisco, CA, 2Laboratory of Brain Development and Repair, The Rockefeller University, New York, NY 1:55 pm E62 Components of ESCRT-III and the AAA-ATPase Vps4 complex are involved in the release of preperoxisomal vesicles from the ER. F.D. Mast1,2, R.A. Saleem1,2, L.R. Miller1,2, R.A. Rachubinski3, J.D. Aitchison1,2; 1Center for Infectious Disease Research, Seattle, WA, 2Institute for Systems Biology, Seattle, WA,3 Cell Biology, University of Alberta, Edmonton, AB 2:00 pm E63 Endolysosomal deficits augment mitochondria pathology in spinal motor neurons of asymptomatic fALS Mice. Y. Xie1, B. Zhou1, M. Lin1, S. Wang1, Z. Sheng1; 1NINDS, NIH, Rockville, MD
l Applications of Cell Biology in the Real World Microsymposium 10: Applications of Cell Biology in the Real World 1:25-2:35 pm Microsymposia Room 2, Learning Center
Moderators: Anupam Das, Albany Medical College; Paul Mungai, American Association for the Advancement of Science; and MONDAY Jessica Polka, Harvard Medical School
1:25 pm Introduction 1:30 pm E64 Inhibiting endothelium directed tumor cell streaming by targeting the HGF/C-Met signaling pathway. E. Leung1, A. Xue2, Y. Wang1, P. Rougerie1, V.P. Sharma1,3, R.J. Eddy1, D. Cox1,4, J.S. Condeelis1,3; 1Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY, 2Pelham Memorial High School, Pelham, NY, 3Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, 4Department of Developmental & Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 1:35 pm E65 Age- and lineage-dependent gene expression is maintained by microenvironment imposed epigenetic states in human mammary epithelial cells. M. Miyano1, M. Stoiber1, M. Stampfer1, J.B. Brown1, M.A. LaBarge1; 1Life Sciences, Lawrence Berkeley National Laboratory, Berkeley, CA 1:40 pm E66 Actin acilitatesf chromosome capture by microtubules in meiosis of starfish oocytes. M. Burdyniuk1, M. Mori2, N. Monnier3, P. Lénárt1; 1Cell Biology and Biophysics, European Molecular Biology Laboratory, Heidelberg, Germany, 2Department of Experimental Genome Research, Osaka University, Osaka, Japan, 3Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 1:45 pm E67 Orthogonal and modular gene regulation using engineered CRISPR/Cas9. A. Didovyk1, B. Borek1, J. Hasty1, L. Tsimring1; 1BioCircuits Institute, University of California, San Diego, La Jolla, CA 1:50 pm E68 Defective endosome maturation and trafficking in models of macular degeneration. K.A. Toops1,2, L. Tan1,3, A. Lakkaraju1,2,3; 1Ophthalmology & Visual Sciences, University of Wisconsin-Madison, Madison, WI, 2McPherson Eye Research Institute, University of Wisconsin-Madison, Madison, WI, 3Division of Pharmaceutical Sciences, University of Wisconsin-Madison, Madison, WI 1:55 pm E69 Drug metabolism and clearance system in normal and malignant plasma cells: relationship to clinical outcome. W. Hassen1,2, A. Kassambara1,3, B. Klein1,3, J. Moreaux1,3; 1Suivi des Therapies Nouvelles, CNRS UPR1142, Institute of Human Genetics; Montpellier, France, Montpellier, France, 2Cellular Biology and Physiology, High Institute of Biotechnology of Monastir, Monastir, Tunisia, 3Department of Biological Haematology, Laboratory for Monitoring Innovative Therapies, Montpellier, France 2:00 pm E70 Altered trafficking of connexin 43 participates to the development of ventricular arrhythmias in cardiomyopathy caused by mutations in A-type lamins gene. C. Macquart1, C. Le Dour2, M. Chatzifrangkeskou1, H.J. Worman2,3, G. Bonne1, A. Muchir1; 1Center of Research in Myology, UPMC - Inserm UMRS 974, CNRS FRE3617, Paris, France, 2Department of Medicine, Columbia University, New York, NY, 3Department of Pathology and Cell Biology, Columbia University, New York, NY
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 85 l Meet the Editor of Molecular Biology of the Cell 1:30-2:15 pm ASCB Booth (721), Learning Center
David Drubin, University of California, Berkeley Editor-in-Chief
Stop by for an informal discussion about the journal with Editor-in-Chief David Drubin. Members of the Molecular Biology of the Cell editorial board will also be available at various times at the ASCB Journals Exhibit Booth (Booth 1226). Stop by the booth or check the Poster Guide for times.
l Even-Numbered Poster Presentations 1:30-3:00 pm Learning Center l Table Talk 2:00-3:00 pm Roundtable Central Section 2, Learning Center
Teaching and Research at a Small, Liberal Arts College Scott Gehler, Augustana College
l Exhibitor Tech Talk 2:00-2:45 pm Theater 1, Learning Center
Cellecta Inc.: CRISPR and RNAi: gene-editing and functional genomic screening approaches Presenter: Paul Diehl, PhD Level: Advanced The CRISPR/Cas9 system is a targeted gene-editing tool adapted from Streptococcus pygogenes and can be used to permanently knock out target genes. It has revolutionized the genomics research along with the earlier tool of RNAi. Genome-wide loss-of- function pooled screens provide a direct approach to identify genes regulating biological responses and find new therapeutic targets. While RNAi screens have proven effective, CRISPR/Cas9 provides an alternative screening approach. To complement our shRNA screening platform, we developed pooled sgRNA libraries for functional CRISPR knockout screens and compared results from sgRNA and shRNA library screens of PDX-derived cell lines. This talk will provide an overview of the functional genomics screening approaches and focus on comparison of the two available methods. l Exhibitor In-Booth Presentation 2:00 pm Booth 923
QImaging: You can have it all: new cameras, new advanced features, new software Presenter: Steven Smith, Product Manager
86 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l Exhibitor Tech Talk 2:00-2:45 pm Theater 2, Learning Center
EMD Millipore: Winning Westerns for cell biologists: get reliable expression data from lysates Presenters: Ivona Strug, PhD, and Sara Gutierrez Level: Intermediate Did you know a reliable Western blot of cell lysates starts before you load your sample on a gel? Learn how cell type, health, cell count, total protein concentration, and lysis buffer composition can affect your semiquantitative Western blot. You’ll learn how to: • Obtain signals that are detectable but not supersaturated • Perform cell lysis/extraction to ensure sample-to-sample consistency • Choose a transfer membrane for excellent protein retention and signal-to-noise ratios • Use IR spectrometry to obtain more reliable quantitation of total protein in cell lysates compared to Bradford/BCA assays • Use IR spectrometry to improve separation and quality of SDS-PAGE As the inventors of PVDF membrane, EMD Millipore knows how informative a good Western can be. Bring your research questions to get the most out of this session! l Career Panel: Science Communication MONDAY 2:00-2:55 pm Career Center Theater, Learning Center
These panelists will give a short talk about their career experiences and then will address individual questions from the audience. Sarah Goodwin, PhD, became the Director of iBiology in 2011 after receiving her PhD in Cell Biology at the University of California, San Francisco. iBiology is a nonprofit organization that produces free videos about biology research and related topics. As Director, she is involved in many different forms of science communication, including working with speakers on their presentations, interviewing scientists, writing grants, writing web content and newsletter articles, and growing the iBiology audience through various outreach activities. She has also helped expand iBiology videos beyond research seminars to include short stories, commentaries, technique talks, and educational videos. She has hired several PhD-level biologists for the iBiology staff, which has grown to three full-time and four part-time team members. Margaret Harmon, PhD, is the Program Director at ScienceMedia, Inc. a company that specializes in science-based eLearning for the life sciences industry. She leads the writing staff in creating multimedia educational training projects in disease states and drug mode of action for clients such as Amgen, Lilly,and Novartis. Harmon has a PhD in Biology from University of California, Los Angeles. Prior to joining ScienceMedia she conducted postdoctoral research in the department of Pharmacology at University of Texas Southwestern Medical Center at Dallas, the Green Center for Reproductive Biology Sciences, and had a research fellowship at the Howard Hughes Medical Institute. Connie M. Lee, PhD, is the Assistant Dean for Basic Science in the Biological Sciences Division at the University of Chicago. She received her PhD in Molecular and Cellular Biology from the University of Wisconsin-Madison. After a postdoctoral fellowship at the Ludwig Maximilian University of Munich, she transitioned into scientific editing and publishing. Lee worked for 11 years as a scientific editor for three different journals, including FEBS Letters, EMBO Journal, and Cell. In 2011, she took a position as Associate Director for the NIH-funded Center for Systems & Synthetic Biology at University of California, San Francisco. In 2014, she moved to her current position at the University of Chicago. Lee also serves as Chair of the ASCB Public Policy Committee, where she continues to develop her interests in the communication of science to the general public and elected politicians, advocating for science funding, and in career opportunities for scientists beyond the bench. Organized by the ASCB Committee for Postdocs and Students (COMPASS) l Exhibitor In-Booth Presentation 2:45 pm Booth 934 Abcam, Inc: Immunostaining: From sample prep through troubleshooting and beyond Presenter: Thomas Novak, Abcam Scientific Support Specialist
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 87 l Organelles and Spatial Organization of the Cell Microsymposium 11: Nucleus Biology and Disease 2:50-4:00 pm Microsymposia Room 1, Learning Center
Moderators: Paulo Caceres, Wayne State University; Anupam Das, Albany Medical College; and Dennis Zimmermann, University of Chicago
2:50 pm Introduction 2:55 pm E71 A role for the nucleus in sarcomere assembly. A.L. Auld1, E.S. Folker1; 1Department of Biology , Boston College, Chestnut Hill, MA 3:00 pm E72 Myonuclear position is regulated by different mechanisms during muscle development and muscle growth. M.A. Collins1, T. Mandigo1, G. Vazquez1, E.S. Folker1; 1Department of Biology, Boston College, Chestnut Hill, MA 3:05 pm E74 Turnover of proteins at the nuclear periphery. A.L. Buchwalter1, M.W. Hetzer1; 1Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 3:10 pm E75 Structural organization of the nuclear lamin isoforms in the nuclear lamina revealed by super resolution microscopy. M. Kittisopikul1, T. Shimi2,3, J. Tran4, A.E. Goldman2, S.A. Adam2, Y. Zheng4, R.D. Goldman2, K. Jaqaman1; 1Biophysics, UT Southwestern, Dallas, TX, 2Cell and Molecular Biology, Northwestern University, Feinberg School of Medicine, Chicago, IL, 3Human Genetics, University of Chicago, Chicago, IL, 4Embryology, Carnegie Institution for Science, Baltimore, MD 3:15 pm E76 Brightness characterization of nuclear envelope proteins by Z-Scan fluorescence fluctuation spectroscopy. J. Hennen1, C.A. Saunders2, E.M. Smith1, J.D. Mueller1, G. Luxton2; 1Physics and Astronomy, University of Minnesota, Minneapolis, MN, 2Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 3:20 pm E77 The nuclear import receptor KPNA7 is critical for neuronal development and function. L.T. Oostdyk1,2, C. Snow2, K. Geffken2, C. Yang2, A.R. Paciorkowski3, M.J. McConnell1, B.M. Paschal1,2; 1Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA, 2Center for Cell Signaling, University of Virginia, Charlottesville, VA, 3Departments of Neurology, Pediatrics and Biomedical Genetics, University of Rochester Medical Center, Rochester, NY
l Signaling and Differentiation Microsymposium 12: Signaling in Differentiation and Cancer 2:50-4:00 pm Microsymposia Room 2, Learning Center
Moderators: Paulo Caceres, Wayne State University; and Hashem Dbouk, University of Texas Southwestern Medical Center
2:50 pm Introduction 2:55 pm E78 Histone deacetylase 1 and 3 inhibition blocks TGFβ-mediated conversion of tumor endothelial cells into myofibroblasts and “re-educates” carcinoma-activated fibroblas. D. Kim1, L. Xiao1, C.A. Otey1, M. Troeste2,3, A.C. Dudley1,3,4; 1Department of Cell Biology & Physiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 2Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 3Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 4McAllister Heart Institute, The University of North Carolina at Chapel Hill, Chapel Hill, NC 3:00 pm E79 Mediator kinase module as a transducer of oncogenic Wnt/β-catenin signaling. A.D. Clark1, M. Oldenbroek1, J.M. Spaeth1, T.G. Boyer1; 1Molecular Medicine, UT Health Sci Center-San Antonio, San Antonio, TX 3:05 pm E80 Sperm TRP-3 channel mediates the timely onset of the fertilization calcium wave in the nematode C. elegans. J. Takayama1, S. Onami1,2; 1Lab. for Developmental Dynamics, RIKEN QBiC, Kobe, Japan, 2NBDC, JST, Tokyo, Japan 3:10 pm E81 Discrete and continuous cell states revealed by single cell sequencing. G. Stanley1, O. Gokce2, B. Treutlein3, S. Quake3,4, T. Sudhof2,4; 1Biophysics, Stanford, Stanford, CA, 2Molecular and Cellular Physiology, Stanford, Stanford, CA, 3Bioengineering, Stanford, Stanford, CA, 4Howard Hughes Medical Institute, Stanford, CA
88 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 3:15 pm E82 Identification of Ran binding protein 6 (RanBP6) as a novel EGFR regulator that is frequently silenced in cancer. W. Hsieh1,2, B. Oldrini1,3, H. Erdument-Bromage4, M. Squatrito3, I.K. Mellinghoff1,5,6; 1Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, NY, 2Pharmacology, Weill Cornell Graduate School of Medical Sciences, New York, NY, 3Seve Ballesteros Foundation Brain Tumor Group, F-BBVA Cancer Cell Biology Program, National Cancer Research Centre, Madrid, Spain, 4Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, 5Neurology, Memorial Sloan Kettering Cancer Center, New York, NY, 6Pharmacology, Weill Cornell Medical College, New York, NY 3:20 pm E83 A novel GSK3-regulated APC:Axin interaction regulates Wnt signaling by driving a catalytic cycle of efficient βcatenin destruction. M. Pronobis1, M. Peifer1,2, N.M. Rusan3; 1Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, 2Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, 3National Heart Blood and Lung Institute, National Institute of Health, Bethesda, MD 3:25 pm E84 Deciphering noncanonical Fzd2 signaling in cancer metastasis. T. Gujral1, M.W. Kirschner1; 1Systems Biology, Harvard Medical School, Boston, MA l Science Discussion Tables 3:00-3:50 pm Roundtable Central Section 3, Learning Center
Whether you’re a student, postdoc, or PI, ASCB will again offer special networking opportunities with senior scientists and peers. Select your interest area and bring your questions to the ASCB Learning Center in the San Diego Convention Center. MONDAY
Table No. Presenter Topic 1 Sue Biggins Mitosis or chromosome segregation 2 Craig Blackstone Endoplasmic reticulum 3 Guillaume Charras Cell and tissue mechanics 4 Michael Rosen Physical mechanisms of protein phase separation 5 Ian Macara Cell polarity, stem cells, and breast cancer 6 Timothy Mitchison Microtubules, mitosis pharmacology, cancer inflammation 7 Carien Niessen Cell adhesion and polarity 8 Jean Schwarzbauer Cell adhesion, integrins, and ECM 9 Ron Vale Science communication and outreach 10 Bernd Pulverer Reproducibility and discoverability l Career Panel: Teaching and University Administration 3:00-3:55 pm Career Center Theater, Learning Center
These panelists will give a short talk about their career experiences and then will address individual questions from the audience.
Karen Hales, PhD, is Professor of Biology at Davidson College where for 15 years she has mentored undergraduate research students and taught intermediate and advanced Genetics courses as well as introductory cell and molecular biology. She earned her PhD at the Stanford University School of Medicine and performed postdoctoral research at the University of North Carolina at Chapel Hill. Her research focuses on spermatogenesis as a context to explore molecular mechanisms of mitochondrial shaping. She served on the Genetics Society of America (GSA) Education Committee and was the inaugural representative from primarily undergraduate institutions to the Drosophila Board, the governing body within the GSA for the fly community. At Davidson she served for three years as elected head of faculty, representing faculty to administration and trustees, a role that encompassed chairing the search committee for a new Vice President for Academic Affairs and Dean of Faculty. Lalitha Jayant, PhD, is an Associate Professor at the Borough of Manhattan Community College where she has been teaching introductory biology and cell biology for 10 years. Her interactive teaching style won her the Phi Theta Kappa award for best professor 2009. She served as a Collegiate Science and Technology Entry Program coordinator for three years, working to recruit and train underrepresented minority students in STEM fields. She continued her work with minority students as a Linkage fellow for the Minorities Affairs Committee program of ASCB. Currently she is redesigning curriculums for Cell Biology and Research Methods courses in order to introduce research at the classroom level in a community college. She is also involved in developing
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 89 student centered teaching and learning activities to increase student retention in introductory biology courses. Virginia (Ginger) Hazen, PhD, is a professional development coordinator at the University of California, San Diego. She attained her PhD in Neuroscience from the University of Southern California and then began a postdoctoral fellowship at University of California, San Diego. Hazen studied the development of mammalian spinal neurons during her scientific training. Throughout her training, she also participated in student government, career development and outreach programs that ultimately led her to pursue a career path in professional development. Hazen became the postdoc professional development coordinator in 2013, a newly created position at UCSD, to prepare postdocs for career success and is a strong advocate for the advancement of postdocs through career development. Organized by the ASCB Committee for Postdocs and Students (COMPASS) l Exhibitor Tech Talk 3:00-4:00 pm Theater 1, Learning Center
ALVÉOLE: Multi-protein printing by light-induced molecular adsorption: application to cell biology Presenters: Pierre-Olivier Strale, Ammar Azioune, Ghislain Bugnicourt, Yohan Lecomte, Makhlad Chahid, and Vincent Studer Level: Intermediate We describe how to perform high resolution multi protein micro-patterning using light-induced molecular adsorption (LIMA). LIMA is based on a photo-initiator able to reverse the antifouling property of polymer brushes when exposed to UV light. The density of adsorbed molecules scales linearly with the dose of UV and the low background allows for the sequential printing of multiple proteins. Our optical set-up (widefield DMD based projection system + epifluorescence microscope) allows us to generate patterns ranging from 500nm to 1 mm and controlled gradients of arbitrary shape. The range of application extends from the single molecule up to the multicellular scale with an exquisite control over protein density. Altogether, LIMA allows for fast high resolution patterning of multiple proteins with applications to biomedical research. l 3Is in South Korea: Initiatives, Innovations, and Information for Bioscience 3:00-4:00 pm Room 23B
Coordinator: Sun-Kyung Lee, Hanyang University and Korean Society for Molecular and Cellular Biology In South Korea, the research base in Bioscience continues to move to new levels. Recently, the South Korean government has been investing in basic science including Bioscience at an unprecedented scale, and more funding opportunities are available to international students, postdocs, and scholars. This session will highlight research interests and job opportunities in more than 50 different universities and research institutes in South Korea, various funding programs to support both Korean nationals and internationals, and what it would be like to be a student, postdoc, or faculty member in the dynamic cultural society of South Korea.
l Exhibitor In-Booth Presentation 3:00-4:00 pm Booth 1235/1237
National Institutes of Health (NIH): National Institute of General Medical Sciences (NIGMS) and Center for Scientific Review (CSR) Presenter: Maqsood Wani, Chief, Cell Biology Integrated Review Group, CSR Get your questions answered about peer review. l Exhibitor In-Booth Presentation 3:00-4:00 pm Booth 335
3H Biomedical AB: Characterization and large scale expansion of human satellite cells Presenter: Mallen Huang
90 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l Exhibitor Tech Talk 3:00-4:00 pm Theater 2, Learning Center
BioTek Instruments: Kinetic live cell imaging Presenter: Paul Held Level: Intermediate Image-based analysis is a powerful tool to assess cellular processes. In this workshop we will demonstrate how the Cytation 5 Cell Imaging MultiMode Reader can be used for live cell kinetic experiments involving: • Gene-specific mRNA expression using SmartFlare probes • Receptor activation through the use of genetically encoded biosensors that detect GPCR second messengers • Miniaturized RNAi knockdown experiments using microarray spotting technology Other applications, such as cell cycle, viability, and migration, conducted in either 2D or 3D spheroids will be discussed. l Large-Scale Data Workshop: Quantitative Analysis and Visualization of Signaling Networks 3:00-6:30 pm Room 31B MONDAY
John Albeck Michael Pargett University of California, Davis University of California, Davis
The overview is open to all attendees. Preregistration is required for the hands-on portion of the workshop.
3:00-4:00 pm Didactic Overview. Open to all ASCB attendees 4:00-6:30 pm Hands-on workshop. Limited to 40 preregistered participants. With current high-throughput methods, it is becoming easy to collect large amounts of experimental data. However, it is not always easy to see what the data mean and to interpret them in the context of a complex biological system. The didactic overview will introduce tools for visualizing trends, calculating useful metrics, and performing basic statistical analysis with large datasets focused on signal transduction networks. We will discuss how to construct models of your system, and how to test them quantitatively with your data. The hands-on workshop will walk participants through the process of analyzing a time-lapse microscopy et with thousands of individual cells expressing multiple signal transduction reporters. This workshop will be particularly useful for those who have already performed image analysis with CellProfiler or equivalent programs, but aren’t sure what to do next, or how to go from the raw data to a completed figure that accurately conveys the key trends. If you are registered for the hands-on workshop, bring a laptop loaded with Matlab software. Many institutions provide free or low-cost Matlab licenses; if yours does not, a free trial version of the software can be obtained at: https://www.mathworks.com/ programs/trials/trial_request.html. Sample datasets for the hands-on workshop will be uploaded to this website by November 15. Please check back and download the sample data prior to the workshop date. If this session is full but people do not show up, we will let people in at the door on a first-come, first-served basis.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 91 l Education Initiative Forum 3:15-3:45 pm Room 24B
Integrating Discovery-Based Research into the Undergraduate Curriculum: Report on an NAS Convocation Sarah C. R. Elgin, Washington University in St. Louis; Erin L. Dolan, Texas Institute for Discovery Education in Science, University of Texas, Austin; Susan R. Wessler, Center for Plant Cell Biology, University of California, Riverside; and Jay Labov, National Academy of Sciences
Recommendation #2 of the 2012 PCAST report, “Engage to Excel,” is to “advocate and provide support for replacing standard laboratory courses with discovery-based research courses.” While this is a challenging goal, course-based undergraduate research experiences (CUREs or CREs), which involve groups of students addressing research problems in the context of a class, have been shown to improve learning, attitudes, and other student outcomes that are predictive of persistence in science. Positive outcomes have been observed for students in all natural science disciplines, at all levels from freshmen to seniors, including underrepresented students. A well-designed CURE includes explicit instructional supports, provides plenty of room for student decision-making, fosters a collaborative atmosphere that includes mentoring by peers and faculty, and provides a mechanism for student communication of their results to a larger audience. This design will be illustrated using three CUREs, both national and local in scope. The Genomics Education Partnership (GEP) is a consortium of faculty largely from primarily undergraduate institutions (~60 schools per year) who work together to mentor their students (>1,000 per year) in improving the sequence and analyzing the genes from the Muller F element (or dot chromosome) of Drosophila. The Freshman Research Initiative (FRI) at UT Austin involves more than 800 freshmen each year who complete a Research Methods course followed by one to two semesters of research in one of 25 different areas, called “research streams.” Longitudinal tracking of FRI students shows they are more likely to graduate on time with a science major than nonparticipating students. Freshmen are also the focus of the Dynamic Genome (DG) course at UC Riverside, offered as an alternative to the Introductory Biology Lab. The DG course will serve over 300 students in 15 sections during 2015-16 with projected expansion to 600 students by 2018. A plug-and-play model allows an increasing number of UCR faculty to take ownership of DG sections that focus on their research interests. Students who complete the DG course have access to additional research opportunities, likely to increase their persistence in a STEM major. The session will consider the opportunities and challenges of implementing and scaling up CUREs to engage large numbers of students, and consider current assessment of such initiatives both locally and nationally. While additional data on long-term impacts is needed, results to date indicate that this instructional approach can improve undergraduates’ success in science. The Convocation, publication of the Convocation report, and some speaker travel costs were supported by the Helmsley Charitable Trust, Sloan Foundation, and Howard Hughes Medical Institute. Organized by the ASCB Education Committee l Networking Happy Hour 4:00-6:00 pm Career Center, Learning Center
This is a networking opportunity to meet with select ASCB exhibitors in an informal setting at the Career Center. Whether you want to establish new contacts, are on the job search, or simply want to learn more about the vendors, we encourage you to drop by and mingle. Cash bar will be available and light snacks will be served. l Kaluza Minisymposium 4:00-6:25 pm Room 32B
4:00 pm Introduction 4:05 pm A4 Target the adaptability of heterogeneous aneuploidy. G. Chen1,2, W.A. Mulla1,3, A. Kucharavy1,3, H. Tsai1,3, B. Rubinstein1, J. Conkright1, S. McCroskey1, D. Bradford1, L. Weems1, J. Haug1, C. Seidel1, J. Berman4, R. Li1,2,3; 1Stowers Institute for Medical Research, Kansas City, MO,2 Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, MO,3 Department of Cell Biology, Johns Hopkins University, Baltimore, MD, 4Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel 4:25 pm A5 Disentangling the Gordian knot: From understanding to preventing the tumorigenicity of human
92 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting pluripotent stem cells. U. Ben-David1,2; 1Department of Genetics, The Hebrew University of Jerusalem, Jerusalem, Israel, 2Cancer Program, Broad Institute of Harvard and MIT, Cambridge, MA 4:45 pm A6 The kinetochore encodes a mechanical toggle-switch to control the spindle assembly checkpoint. P. Aravamudhan1, A.P. Joglekar2, A.A. Goldfarb2; 1Department of Pediatrics, Vanderbilt University, Ann Arbor, TN, 2Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 5:05 pm A7 Cell biology of cheating - mechanism of selfish element transmission through asymmetrical meiosis. L. Chmatal1, K. Yang1, R.M. Schultz1, M.A. Lampson1; 1Biology, University of Pennsylvania, Philadelphia, PA 5:20 pm A8 Mechanisms of target specificity in eukaryotic gene silencing pathways. P.A. Dumesic1, H.D. Madhani1; 1Biochemistry and Biophysics, University of California, San Francisco, CA 5:35 pm A9 Epigenetic Transmission of a Gene Repression Memory Across Generations and During Development. L.J. Gaydos1, A. Rechtsteiner2, T. Egelhofer2, S. Strome2; 1Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, 2Molecular, Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 5:50 pm A10 Investigation of coordinated stem cell behaviors in the skin by live imaging. K.R. Mesa1, V. Greco1; 1Genetics, Yale University, New Haven, CT 6:05 pm A11 Molecular mechanisms of vinculin activation and nanoscale organization at focal adhesions. L.B. Case1, M.A. Baird1,2, G. Shtengel3, S.L. Campbell4, M.W. Davidson2, H.F. Hess3, C.M. Waterman1;
1Cell Biology and Physiology Center, NHLBI, National Institutes of Health, Bethesda, MD, 2National MONDAY High Magnetic Field Laboratory, The Florida State University, Tallahassee, FL,3 , Janelia Farm Research Campus, HHMI, Ashburn, VA, 4Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC l Cell Cycle and Cell Division Minisymposium 7: Centrosomes and Spindles 4:00-6:25 pm Room 28D
Co-Chairs: Daniela Cimini, Virginia Tech; and Mary Dasso, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH
4:00 pm Introduction 4:05 pm M60 Proper organization of the interphase centrosome structure through the coordinated activities of Centrosomin and Pericentrin-like protein is essential for viability. D.A. Lerit1, H.A. Jordan1, J.S. Poulton2, C.J. Fagerstrom1, G.J. Brian1, M. Peifer2, N.M. Rusan1; 1Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, 2Biology Department, University of North Carolina at Chapel Hill, Chapel Hill, NC 4:25 pm M61 A splice variant of Centrosomin converts mitochondria to MTOCs to facilitate sperm morphogenesis in Drosophila. J.V. Chen1, T.L. Megraw1; 1Biomedical Sciences, Florida State University, Tallahassee, FL 4:45 pm M62 A microscope adaptation that allows high-speed 3D imaging from a single plane of focus. C.J. Cogswell1, J. Yu1, R.N. Zahreddine1, S. Chen1, J. Xing1, R.H. Cormack1, J.S. Tyler2, M. Winey2; 1Electrical, Computer and Energy Engineering, University of Colorado, Boulder, CO, 2Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 5:05 pm M63 Identification of a mitotic SKAP isoform reveals roles in astral microtubule behavior and spindle positioning. D.M. Kern1,2, P.K. Nicholls2, D.C. Page1,2, I.M. Cheeseman1,2; 1MIT, Cambridge, MA, 2Whitehead Institute, Cambridge, MA 5:25 pm M64 Microcephaly protein Asp focuses the spindle microtubule minus ends independent of Ncd motor protein. A. Ito1, G. Goshima1; 1Division of Biological Science, Nagoya University, Nagoya, Japan 5:45 pm M65 The Golgi matrix protein GM130 participates in spindle assembly by activating TPX2 and capturing microtubules. J. Wei1,2, Z. Zhang3,4, R. Wynn5, J. Seemann1; 1Cell Biology, UT Southwestern, Dallas, TX, 2Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, 3Pharmacology, UT Southwestern, Dallas, TX, 4Institute of Life Sciences, Southeast University, Nanjing , China, 5Internal Medicine, UT Southwestern, Dallas, TX 6:05 pm M66 EML3 participates in mitotic spindle assembly by regulating the acentrosomal microtubule nucelation. J. Luo1, Z. Deng1, B. Yang1, Q. Jiang1, C. Zhang1; 1The MOE Key Laboratory of Cell
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 93 Proliferation and Differentiation and the State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing, China 6:12 pm M67 Centrosome age regulates kinetochore microtubule stability and biases chromosome mis- segregation. I. Gasic1, P. Meraldi1; 1Cellular Physiology and Metabolism Department, University of Geneva, Geneva, Switzerland 6:19 pm M68 Temporal and spatial dynamics of spindle midzone assembly revealed by lattice light sheet microscopy. S. Forth1, P. Verma1, M. Sen1, M.C. Pamula1, W.R. Legant2, E. Betzig2, T.M. Kapoor1; 1Laboratory of Chemistry and Cell Biology, Rockefeller University, New York, NY, 2Janelia Research Campus, HHMI, Ashburn, VA
l Membrane Organization, Dynamics, Traffic, and Regulation Minisymposium 8: Lipid Organization, Transport, Composition, and Phosphoinositides 4:00-6:25 pm Ballroom 20A
Co-Chairs: Tamas Balla, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH; and Satyajit Mayor, National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India
4:00 pm Introduction 4:05 pm M69 Local control of membrane composition by Integrin receptors. J.K. Mathew1, S. Mayor1; 1National Centre for Biological Sciences (TIFR), Bangalore, India 4:25 pm M70 Interplay between membrane traffic and sphingolipid organization in the plasma membrane. M.L. Kraft1, H.A. Klitzing1, R. Kim1, P.K. Weber2, J. Zimmerberg3; 1School of Chemical Sciences, University of Illinois, Urbana Champaign, Urbana, IL, 2Chemical Biology and Nuclear Science, Lawrence Livermore National Laboratory, Livermore, CA,3 Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 4:45 pm M71 PI4P/phosphatidylserine countertransport at ORP5- and ORP8-mediated ER-plasma membrane contacts. J. Chung1, F. Torta2, K. Masai1, L. Lucast1, H. Czapla1, L.B. Tanner2, P. Narayanaswamy2, M.R. Wenk2, F. Nakatsu1, P. De Camilli1; 1Department of Cell Biology, Yale School of Medicine/ HHMI, New Haven, CT, 2Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 5:05 pm M72 A tight metabolic relationship between phosphatidylinositol 4-phosphate turnover and phosphatidyserine synthesis. M. Sohn1, P.T. Ivanova2, A.H. Brown2, Y. Kim1, T. Balla1; 1NICHD, National Institutes of Health, Bethesda, MD,2 Department of Pharmacology and Biochemistry, Vanderbilt-Ingram Cancer Center, Vanderbilt University, School of Medicine, Nashville, TN 5:25 pm M73 The acyltransferase LYCAT regulates phosphoinositides and specific stages of endomembrane traffic. L.N. Bone1, R.M. Dayam1, R.J. Botelho1, C.N. Antonescu1; 1Department of Chemistry and Biology, Ryerson University, Toronto, ON 5:45 pm M74 Lipid engineering approaches reveal cellular roles for membrane viscosity. I. Budin1, J.D. Keasling2; 1Miller Institute, University of California, Berkeley, Berkeley, CA, 2Chemical Engineering, University of California, Berkeley, Berkeley, CA 6:05 pm M75 Lipid binding by Osh4p, an OSBP homologue, is required for a discrete step in polarized exocytosis. R.J. Smindak1, D.M. Hyatt1, K.G. Kozminski1,2, R.C. Deutscher1; 1Biology, University of Virginia, Charlottesville, VA,2 Cell Biology, University of Virginia, Charlottesville, VA 6:12 pm M76 Sac1 regulates microtubule stability and trafficking of cell surface adhesion molecules in the developing Drosophila eye. L.M. Del Bel1,2, R. Wilk1, J. Burgess1,2, G. Polevoy1, H. Wei1, J.A. Brill1,2; 1Cell Biology, The Hospital for Sick Children, Toronto, ON, 2Molecular Genetics, The University of Toronto, Toronto, ON 6:19 pm M77 Dietary fats remodel the plasma membrane lipidome to regulate the stability of membrane rafts. K.R. Levental1, J.H. Lorent1, X. Lin1, A. Gorfe1, I. Levental1; 1Integrative Biology and Pharmacology, The University of Texas Health Science Center at Houston, Houston, TX
94 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l Cytoskeleton, Motility, and Cell Mechanics Minisymposium 9: Microtubule-Based Motility and Dynamics 4:00-6:25 pm Ballroom 20C
Co-Chairs: Samara Reck-Peterson, University of California, San Diego; and Trina Schroer, Johns Hopkins University
4:00 pm Introduction 4:05 pm M78 A proteomics survey reveals the human cytoplasmic dynein transportome. W.B. Redwine1, I. Hollyer2, M. DeSantis2, S.L. Reck-Peterson1,2; 1Cell Biology, Harvard Medical School, Boston, MA, 2Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 4:25 pm M79 Dynarrestin is a novel small molecule inhibitor of cytoplasmic dynein. T. Yeh1, S. Höing2,3, M. Baumann3, H.C. Drexler4, S.A. Ketcham1, B. Klebl3, H.R. Schöler2,5, H. Waldmann6, J.L. Sterneckert2,7, T.A. Schroer1; 1Biology, Johns Hopkins University, Baltimore, MD, 2Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster, Germany, 3Lead Discovery Center GmbH, Dortmund, Germany, 4Bioanalytical Mass Spectrometry, Max Planck Institute for Molecular Biomedicine, Münster, Germany, 5Medical Faculty, University of Münster, Münster, Germany, 6Chemical Biology, Max Planck Institute for Molecular Physiology, Dortmund, Germany, 7DFG-Research Center for Regenerative Therapies Dresden, Cluster of Excellence / Technische Universität Dresden, Dresden, Germany
4:45 pm M80 IFT-dynein dynamics in vivo: an ensemble and single-molecule quantification. J. Mijalkovic1, MONDAY B. Prevo1, P.J. Mangeol1, F. Oswald1, E.J. Peterman1; 1Physics and Astronomy, VU Amsterdam, Amsterdam, Netherlands 5:05 pm M81 The C. elegans Ninein-related protein NOCA-1 functions coordinately with γ-tubulin and in parallel to patronin to assemble non-centrosomal microtubule arrays. S. Wang1,2, S. Quintin3,4, R.A. Green1, D.K. Cheerambathur1, S.D. Ochoa1, D. Wu1, B. Prevo1, A.B. Desai1,5, K. Oegema1,5; 1Ludwig Institute for Cancer Research, La Jolla, CA, 2Biomedical Sciences Graduate Program, University of California, San Diego, La Jolla, CA, 3CNRS, Université de Strasbourg, Illkirch, France, 4CNRS, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France, 5Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 5:25 pm M82 Towards an understanding of the molecular mechanism of the unique biomechanical properties of the yeast kinesin-8 Kip3. H. Arellano-Santoyo1,2,3, E. Stokasimov1,2,3, X. Su3,4, D.S. Pellman1,2,3; 1Pediatric Oncology, Dana Farber Cancer Institute, Boston, MA, 2Cellular Biology, Harvard Medical School, Boston, MA, 3Howard Hughes Medical Institute, Boston, MA,4 School of Medicine, UCSF, San Francisco, CA 5:45 pm M83 A small GTPase ARL-8 regulates synapse formation by unlocking the autoinhibition of the axonal kinesin UNC-104/KIF1A. K. Shen1,2, S. Niwa1,3; 1Biology, Stanford University, Stanford, CA, 2Howard Hughes Medical Institute, Stanford, CA, 3Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Sendai, Japan 6:05 pm M84 Interplay between kinesin-1 and cortical dynein during axonal outgrowth and microtubule organization in Drosophila neurons. U. del Castillo1, M. Winding1, W. Lu1, V.I. Gelfand1; 1Dept. of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 6:12 pm M85 Purified ecombinantr human tubulin isotypes show distinct polymerization properties in vitro. M.C. Pamula1, S. Ti1, T.M. Kapoor1; 1Laboratory of Chemistry and Cell Biology, The Rockefeller University, New York, NY 6:19 pm M86 Mechanism of microtubule lumen entry for the α-tubulin acetyltransferase enzyme αTAT1. C.E. Coombes1, A. Yamamoto1, M. McClellan1, M. Plooster1, J. Alper2,3, G. Luxton1, J. Howard2, M. Gardner1; 1Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN, 2Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 3Physics and Astronomy, Clemson University, Clemson, SC
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 95 l Signaling and Differentiation Minisymposium 10: New Technologies and Immuno-Signaling 4:00-6:25 pm Room 30C
Chair: Peter Yingxiao Wang, University of California, San Diego
4:00 pm Introduction 4:05 pm M87 LOV and Zdark, a protein pair that dimerize selectively in the dark, provide a versatile optogenetic toolbox. H. Wang1, K. Bryant1, C. Der1, K.M. Hahn1; 1Pharmacology, University of North Carolina Chapel Hill, Chapel Hill, NC 4:25 pm M88 *Using subcellular optogenetics to uncover signaling dynamics that control immune cell migration. P.R. O’Neill1, V. Kalyanaraman1, N. Gautam1,2; 1Anesthesiology, Washington University School of Medicine, St. Louis, MO, 2Genetics, Washington University School of Medicine, St. Louis, MO 4:45 pm M89 Engineered regulation of protein tyrosine phosphatases in living cells. J.B. Klomp1, V. Huyot1, A.V. Karginov1, A. Ray1; 1Pharmacology, University of Illinois at Chicago, Chicago, IL 5:05 pm M90 Mapping the human calcineurin phosphatase signaling network through global identification of short linear motifs that mediate substrate recognition. M.S. Cyert1, J. Roy1, N. Damle1, C.P. Wigington1, P.M. Kim2,3, N.E. Davey4, Y. Ivarsson5; 1Biology, Stanford University, Stanford, CA, 2Department of Molecular Genetics, University of Toronto, Donnelly Centre, Toronto, Canada, 3Department of Computer Science, University of Toronto, Donnelly Centre, Toronto, Canada, 4Conway Institute of Biomolecular and Biomedical Research, University College Dublin , Dublin, Ireland, 5Department of Chemistry, Uppsala University, Uppsala, Sweden 5:25 pm M91 Image-driven analysis of molecular inter-regulation in live cells. S. Lu1, P. Wang1; 1Bioengineering, UCSD, La Jolla, CA 5:45 pm M92 TFE3 and TFEB regulate autophagy induction, lysosomal biogenesis, and cytokine production in activated macrophages. O.A. Brady1, H.I. Diab1, J.A. Martina1, L. Sun1, J. Lim2, N. Raben2, R. Puertollano1; 1Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, Bethesda, MD, 2Laboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Bethesda, MD 6:05 pm M93 A novel master adaptor for toll-like receptors tails pro-inflammatory responses. L. Luo1,2, N.J. Bokil1,2, A.A. Wall1,2, M.J. Sweet1,2, J.L. Stow1,2; 1Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia, 2IMB Centre for Inflammation Research and Disease, The University of Queensland, Brisbane, Australia
*Patrick O’Neill is the recipient of the Molecular Biology of the Cell Paper of the Year Award. l Cell Biology of Genetic Information Minisymposium 11: Nuclear Mechanics and Transport 4:00-6:25 pm Room 29C
Co-Chairs: Abby Dernburg, University of California, Berkeley/HHMI; G.V. Shivashankar, National University of Singapore; and Karsten Weis, ETH Zürich, Switzerland
4:00 pm Introduction 4:05 pm M94 Nuclear mechanics and genome regulation. G. Shivashankar1; 1Mechanobiology Institute, National University of Singapore, Singapore, Singapore 4:25 pm M95 Chromatin and lamin A dominate two different regimes of nuclear mechanical force response. A.D. Stephens1, E. Banigan1,2, L. Almassalha3, Y. Stypula-Cyrus3, V. Backman3, S.A. Adam4, R.D. Goldman4, J.F. Marko1,2; 1Department of Molecular Biosciences, Northwestern University, Evanston, IL, 2Department of Physics and Astronomy, Northwestern University, Evanston, IL, 3Department of Biomedical Engineering, Northwestern University, Evanston, IL, 4Feinberg School of Medicine, Northwestern University, Chicago, IL 4:45 pm M96 Formin mDia2 mediated nuclear actin regulates CENP-A deposition at centromeres. C. Liu1, Y. Mao1; 1Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY
96 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 5:05 pm M97 In vivo single particle imaging of nuclear mRNA export in budding yeast. A. Lari1, C. Smith2, C. Derrer3, A. Ouwehand2,4, A. Rossouw2,4, M. Huisman2,4, T. Dange4, M. Hopman4, A. Joseph2, D. Zenklusen5, K. Weis3,6, D. Grunwald2,4, B. Montpetit1; 1Department of Cell Biology, University of Alberta, Edmonton, AB, 2Department of Biochemistry and Molecular Pharmacology, RNA Therapeutics Institute, University of Massachusetts Medical School , Worcester, MA, 3Department of Biology, Institute of Biochemistry, ETH Zurich, Zurich, Switzerland,4 Bionanoscience, Kavli Institute of NanoScience, Delft University of Technology, Delft, Netherlands, 5Départment de Biochimie et médecine moléculaire, Université de Montréal, Montréal, QC, 6Department of Cell and Developmental Biology, University of California, Berkeley, Berkeley, CA 5:25 pm M98 Influenza virus mRNA splicing and export through nuclear speckles. A. Mor1, A. White1, R. Munoz- Moreno2, A. Garcia-Sastre2, B.M. Fontoura1; 1Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, 2Department of Microbiology, Department of Medicine, Division of Infectious Diseases, Global Health and Emerging Pathogens Institute, Mount Sinai School of Medicine, New York, NY 5:45 pm M99 Nucleic acid-programmed RNA tracking in living cells with CRISPR/Cas9. D.A. Nelles1,2, M. Fang1, M.R. O’Connell3, S.J. Markmiller1, J.A. Doudna3, G. Yeo1,2,4; 1Cellular and Molecular Medicine, University of California, San Diego, San Diego, CA, 2Materials Science and Engineering Graduate Program, University of California, San Diego, San Diego, CA, 3Departments of Molecular and Cell Biology and Chemistry, University of California, Berkeley, and Howard Hughes Medical Institute, 4Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore 1 1 1 1
6:05 pm M100 Regulation of intracellular transport and diffusion. K. Weis , R.P. Joyner , J.H. Tang , E.M. Dultz ; MONDAY 1Department of Biology, ETH Zurich, Zurich, Switzerland
l Organelles and Spatial Organization of the Cell Minisymposium 12: Organelle Dynamics, Structure, and Function 4:00-6:25 pm Ballroom 20D
Co-Chairs: Martin Jonikas, Carnegie Institute for Science and Stanford School of Medicine; and Susanne Rafelski, University of California, Irvine, and Allen Institute for Cell Science, Seattle
4:00 pm Introduction 4:05 pm M101 Biogenesis of the eukaryotic carbon-concentrating organelle. L.C. Mackinder1, M.T. Meyer2, T. Mettler-Altmann3, V.K. Chen1,4, M.C. Mitchell2, O.D. Caspari2, E.S. Freeman Rosenzweig1,4, L. Pallesen1, A. Itakura1,4, G. Reeves1, R. Roth5, F. Sommer3, S. Geimer6, T. Mühlhaus 3, M. Schroda3, U. Goodenough5, M. Stitt3, H. Griffiths2, M.C. Jonikas1; 1Plant Biology, Carnegie Institution for Science, Stanford, CA, 2Department of Plant Sciences, University of Cambridge, Cambridge, UK, 3Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany, 4Department of Biology, Stanford University, Stanford, CA, 5Department of Biology, Washington University, St. Louis, St. Louis, MO, 6Cell Biology Electron Microscopy, University of Bayreuth, Bayreuth, Germany 4:25 pm M102 A sol-gel transition of the cytoplasm driven by adaptive intracellular pH changes promotes entry into dormancy. M.C. Munder1,2, D. Midtvedt2, T.M. Franzmann1, E. Nüske1, L. Malinovska1, O. Otto3, E. Ulbricht3, J. Guck3, V. Zaburdaev2, S. Alberti1; 1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany, 2Max Planck Institute for the Physics of Complex Systems, Dresden, Germany, 3Biotechnology Center, Technische Universität Dresden, Dresden, Germany 4:45 pm M103 Mitochondrial fission and fusion dynamics are required to generate network topologies that are robust, efficient and well-distributed in budding yeast cells. M.P. Viana1,2, I.A. Mueller1,2, C. Goul1,2, S.M. Rafelski1,2; 1Developmental and Cell Biology, University of California, Irvine, Irvine, CA, 2Center for Complex Biological Systems, University of California, Irvine, Irvine, CA 5:05 pm M104 Dynamic actin cycling through mitochondrial subpopulations regulates mitochondrial homeostasis. A.S. Moore1, Y.C. Wong1, E.L. Holzbaur1; 1Physiology, University of Pennsylvania, Philadelphia, PA 5:25 pm M105 MICOS coordinates with respiratory complexes and lipids to establish mitochondrial inner membrane architecture. J.R. Friedman1, A. Mourier2, J. Yamada1, J. McCaffery3, J. Nunnari1; 1MCB, University of California, Davis, CA, 2Department of Mitochondrial Biology, Max Planck
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 97 Institute for Biology of Ageing, Cologne, Germany,3 Integrated Imaging Center, Johns Hopkins University, Baltimore, MD 5:45 pm M106 Multiple dynamin amilyf members collaborate to drive mitochondrial division. J.E. Lee1, G. Voeltz1; 1Molecular, Cellular, Developmental Biology, University of Colorado, Boulder, Boulder, CO 6:05 pm M107 Changing the paradigm for Drp1 oligomerization during mitochondrial fission: roles for actin and myosin II at specific stages in the process. W. Ji1, A. Hatch1, H. Higgs1; 1Biochemistry, Dartmouth Medical School, Hanover, NH 6:12 pm M108 SPD-2/CEP192 and CDK are limiting for microtubule organizing center function at the centrosome. R. Yang1, J.L. Feldman1; 1Biology, Stanford University, Stanford, CA 6:19 pm M109 Temporal and spatial dynamics of centrosome assembly in C. elegans. T. Laos1, G. Cabral1, A. Dammermann1; 1Max F. Perutz Laboratories, University of Vienna, Vienna, Austria l Exhibitor Tech Talk 4:15-5:15 pm Theater 2, Learning Center
Applied Scientific Instrumentation Inc.: Advances in dual inverted selective plane microscopy (diSPIM) and laser technology Presenters: Dr. Gary Rondeau, Applied Scientific Instrumentation Inc.; Dr. Larry Shi and Dr. Dan Christensen, TOPTICA Photonics, Inc. Level: Introductory Dr. Gary Rondeau will discuss the operation and advantages of the dual inverted selective plane microscopy (diSPIM) system that was developed in collaboration with Dr. Hari Shroff and his group at the NIH/NIBIB. Recent developments will be discussed, which include operation modes for stage scanning, multi-color acquisition, and auto-focus during long acquisitions. Dr. Larry Shi and Dr. Dan Christensen will discuss features and benefits of TOPTICA’s broad portfolio of multi laser engine (MLE) products, specifically how it improves light sheet microscopy in the diSPIM system. Equipped with a proprietary, fully automated laser alignment system (COOL AC), the TOPTICA MLE is the only commercially available, turn-key laser light engine that guarantees consistent optical power over time. l Exhibitor Tech Talk 4:15-5:15 pm Theater 1, Learning Center
4:15-4:35 pm, NanoSurface Biomedical, Inc.: Nano-engineering cell phenotype and function with aligned nanopatterned cell culture dishes Presenters: Elliot Fisher and Sangwook Choi Level: Introductory NanoSurface Biomedical’s ANFS (Anisotropically Nano-Fabricated Substratum) technology integrates mechanical characteristics of the in vivo cellular micro-environment into a familiar in vitro platform for daily use by researchers. Featuring topographical resemblance to the native extracellular matrix, NanoSurface Biomedical’s nanopatterned substrates provide a topographical and mechanical niche for cells, facilitating growth as if in vivo. High resolution microscopic images of cells cultured in ANFS dishes are made possible through the use of low auto-fluorescence surface materials; these products are ideal for confocal microscopy, phase contrast microscopy, live cell imaging and micro-manipulations. Currently available in 35mm culture dishes, 6-well plates, and 24-well plates.
4:35-4:55 pm, Montana Molecular: Single fluorescent protein-based biosensors for detecting Gi, Gs, and Gq-coupled pathways of GPCR signaling Presenter: Anne Marie Quinn Level: Intermediate G protein coupled receptors produce a myriad of effects through a variety of effectors. To study these effects, we created genetically encoded fluorescent biosensors for cAMP and DAG. These sensors are analogous to the GCaMP and GECO Ca2+ sensors, in that the analyte binding domains are fused to a single fluorescent protein. A major advantage of single fluorescent protein sensors is that they can be combined for simultaneous measurement of multiple analytes. These sensors are robust enough for detection with epifluoresence microscopy or fluorescence plate readers. The sensors are packaged in viruses for delivery to mammalian cells. Changes in fluorescence are reproducible from cell to cell. cADDIs, a cAMP sensor, detects both Gs and Gi-coupled responses. Upward and Downward DAG sensors detect Gq signaling.
98 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 4:55-5:15 pm, VitaScientific: New tools for cell biology from VitaScientific.com Presenter: Sean Yu Level: Intermediate VitaScientific is a life science supplier based in Maryland, USA. We constantly strive to bring the highest quality, most innovative products from around the globe to biomedical researchers in their quest to better the human condition. We pride ourselves on our customer service, technical support, and dedication to ensuring your experiment is a success. We would like to introduce: • A new electroporation technology that ensures uniformly high efficiency • A transfection product line that routinely outperforms the Lipofectamines • The only commercially CLARITY tissue clearing system for cutting edge 3-D imaging • Dedicated semi-automated Western Blotting imager, digital fluorescence microscope with high quality imaging sensors Visit us at www.VitaScientific.com for more innovative research tools for your lab.
l Exhibitor Tech Talk 5:30-7:45 pm Theater 2, Learning Center
Nanolive SA: Product Launch: 3D Cell Explorer, revolutionary microscope able to image living cells instantly, in 3D and 4D MONDAY Presenter: Yann Cotte Level: Intermediate Launching the 3D Cell Explorer, its revolutionary microscope able to image living cells instantly, in 3D and 4D (http://nanolive.ch/ applications/), without labels at a resolution below the diffraction limit of light (200nm). The refractive index distribution within the cell is measured at each pixel and the researcher can decide which parts of the cell to visualize by digitally staining them in contrasting colors, without interfering with the cell’s normal physiology (http://nanolive.ch/software/). An engaging keynote from Nanolive’s CEO, Yann Cotte, will be followed by a reception open to all ASCB participants (www.ascb. org/2015meeting/tech-launches/).
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 99 l Emerging Topic Symposium - Mitochondria and Cancer Cell Biology 6:45-8:00 pm Ballroom 20D
Jointly supported by the National Cancer Institute, NIH and the American Society for Cell Biology
Jodi Nunnari Michael Graham Espey University of California, Davis National Cancer Institute Co-Chairs: Jodi Nunnari, University of California, Davis, and Michael Graham Espey, National Cancer Institute, Rockville, MD Mitochondria serve to define cellular morphology and functional states. Cellular processes that rely on mitochondrial networks are altered or destabilized by stresses commonly experienced by cancer cells (e.g., mutation, hypoxia, therapeutics). Recognition of these patterns and how they are exploited and selected upon for cancer cell survival and resistance reveals new insights into the mechanistic underpinnings of the mitochondrion as a dynamic integrative determinant of cellular function and phenotype. This session seeks to gain interdisciplinary insights through discussion of basic mechanistic experiments that can inform an understanding of cancer etiology and progression, which will ultimately lead to improved treatment strategies. Speakers:
Susanne Rafelski Gerald S. Shadel Jeremy Rich Jerry Chipuk University of California, Yale School of Cleveland Clinic Mount Sinai Hospital, Irvine, and the Allen Institute Medicine Icahn Medical Institute for Cell Science
Open questions and new approaches at the intersection of mitochondrial network dynamics and cancer cell biology. Susanne Rafelski, University of California, Irvine, and the Allen Institute for Cell Science, Seattle Mitochondrial DNA stress in innate immunity and cancer. Gerald S. Shadel, Yale School of Medicine, New Haven, CT Metabolic control of cancer stem cells. Jeremy Rich, Cleveland Clinic, Cleveland, OH Chronic mitochondrial division in cancer signaling, treatment, and prognosis. Jerry Chipuk, Mount Sinai Hospital, Icahn Medical Institute, New York, NY
100 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Tuesday December 15, 2015
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 101 7:30 am-6:30 pm Registration Open Registration Area 7:30-8:00 pm Career Center Open Learning Center
8:00-9:30 am Symposium 5: Bending Nature to Our Purposes: Engineering of Ballroom 20BC Cells and Tissues 8:15-9:15 am Exhibitor Tech Talk Theater 1, Learning Center Sciencell Research Laboratories: Maximize your cell culture: technical tips for primary cells, pluripotent stem cells, and cell analyses 9:30-4:30 pm ASCB Learning Center (Exhibit Hall) Open Learning Center 9:30-11:30 am Morning Refreshment Break Learning Center
9:30-10:30 am Exhibitor Tech Talk Theater 2, Learning Center Reveal Biosciences: Quantitative multiplexed in situ hybridization (ISH) in tissue and cell-based assays One World Lab: New technologies to assess quality and compatibility of commercial antibodies from multiple vendors EditBIOMED: What can a scientific editing service do for you? 9:45-10:45 am Symposium 6: Going the Distance: Determining Size and Ballroom 20BC Spacing of Biological Structures 10:00 am-12:00 pm One-Minute, All-Video Elevator Speech Contest ASCB Booth (721), Learning Center 10:00-11:00 am Table Talk Roundtable Central Section 1, Transitioning to a Postdoc Position Learning Center 10:30 am-12:30pm Building a Successful Research Program with Career Center Theater, Undergraduates—A Roadmap Learning Center 10:45-11:45 am Exhibitor Tech Talk Theater 1, Learning Center Wiley: Want people to read your paper? Here’s how to optimize your chances… 10:45-11:45 am Exhibitor Tech Talk Theater 2, Learning Center Tecan: Rapid generation of compound serial dilutions for cell assays using an inkjet based dispensing technology 10:45 am-12:00 pm Women in Cell Biology (WICB) Committee Awards Presentation and Room 32B Mentoring Theater: Who Me? I’m Not Biased. Embracing Diversity to Improve Creativity 10:45 am-12:00 pm Morning with the Editors: An Open Discussion with Journal Editors Room 29C 11:00 am-12:00 pm Advocacy Toolbox: How to Start and Sustain a Policy Advocacy Group Room 24A
Daily Schedule—Tuesday, December 15 11:00 am-12:00 pm LGBTQ Diversity Session Room 24B 11:00 am-12:00 pm Brazil: Opportunities for the Young: Fellowships, Resources, and Interactions Room 23B 11:00 am-12:00 pm Table Talk Roundtable Central Section 3, Bringing Research into the Undergraduate Curriculum Learning Center 11:30 am-12:30 pm Table Talk Roundtable Central Section 1, Developing Case Studies in Cell Biology Learning Center 12:00-12:45 pm Exhibitor Tech Talk Theater 2, Learning Center EMD Millipore: New advances in microfluidic control of cellular microenvironment with uninterrupted imaging allows for highly controllable, long-term, more in vivo-like cell culture studies 12:00-1:30 pm Odd-Numbered Poster Presentations Learning Center 12:00-1:10 pm Microsymposium 13: Morphology of the Cytoskeleton Leading to Microsymposia Room 1, Morphology in Development Learning Center
12:00-1:10 pm Microsymposium 14: Actin Cytoskeleton Dynamics Microsymposia Room 2, Learning Center 12:00 pm Exhibitor In-Booth Presentation Booth 934 Abcam, Inc: Immunostaining: From sample prep through troubleshooting and beyond 12:00-4:00 pm Afternoon Refreshment Break Learning Center
102 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 12:00-12:45 pm Exhibitor Tech Talk Theater 1, Learning Center GE Healthcare: Quantitative Western blotting: improving your data quality and reproducibility 1:00-1:55 pm Navigating the New NIH Biosketch Career Center Theater, Learning Center 1:00-1:45 pm Exhibitor Tech Talk Theater 1, Learning Center Beckman Coulter Life Sciences: Extracellular vesicle isolation by flow cytometric sorting and characterization by analytical ultra-centrifugation and dynamic light scatter 1:00-1:45 pm Exhibitor Tech Talk Theater 2, Learning Center Andor: Fast and sensitive camera technologies for microscopy 1:00-2:00 pm Exhibitor In-Booth Presentation Booth 1235/1237 National Institutes of Health (NIH): National Institute of General Medical Sciences (NIGMS) and Center for Scientific Review (CSR)
1:25-2:35 pm Microsymposium 15: Membrane Regulation and Signaling Microsymposia Room 1, Daily Schedule—Tuesday, December 15 Learning Center 1:25-2:35 pm Microsymposium 16: Cell Biology of Genetic Information Microsymposia Room 2, Learning Center 1:30-2:30 pm Table Talk Roundtable Central Section 1, Crafting a Research Statement That Is Undergraduate Research Friendly Learning Center 1:30 pm-3:00 pm Even-Numbered Poster Presentations Learning Center 2:00-2:55 pm Career Panel: Industry and Sales Career Center Theater, Learning Center 2:00-2:45 pm Exhibitor Tech Talk Theater 1, Learning Center Thermo Fisher Scientific: Automated protein detection - the future of Westerns 2:00-2:45 pm Exhibitor Tech Talk Theater 2, Learning Center Essential Pharmaceuticals: Variables in FBS impact cell performance, reproducibility, and relevant experimental results 2:50-4:00 pm Microsymposium 17: Spindle Assembly and Chromosome Dynamics Microsymposia Room 1, Learning Center 2:50-4:00 pm Microsymposium 18: Cell Mechanics and Adhesion Microsymposia Room 2, Learning Center 3:00-4:00 pm Celldance Videos, Elevator Speech Contest Finals, and Comic Contest Awards Theater 1, Learning Center 3:00-4:00 pm Exhibitor Tech Talk Theater 2, Learning Center Essen BioScience: Novel kinetic live cell imaging assays for T cell killing of tumor cells 3:00-4:00 pm Research in Cell Biology in France: Opportunities and Tips Room 23B 3:00-4:00 pm Career Panel: Research Development and Grant Administration Career Center Theater, Learning Center 3:00-3:50 pm Science Discussion Tables Roundtable Central Section 3, Learning Center 3:00-4:00 pm ASCB Business Meeting ASCB Booth (721) Theater, Learning Center 3:00-4:00 pm Exhibitor In-Booth Presentation Booth 1235/1237 National Institutes of Health (NIH): National Institute of General Medical Sciences (NIGMS) and Center for Scientific Review (CSR) 3:00-6:25 pm Large-Scale Data Workshop: Image Analysis in Quantitative Microscopy Room 31B 3:00-6:30 pm Subgroup P: The Cellular and Molecular Basis of Invasive Metastatic Cancer Room 32B 3:15-3:45 pm Education Initiative Forum Room 24B Using Visual Networks to Map the Systems Thinking Literature 3:30 pm-4:00 pm Poster Removal Learning Center
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 103 4:00-6:25 pm Applications of Cell Biology in the Real World Room 29C Minisymposium 13: Applications of Cell Biology 1
Cell Cycle and Cell Division Ballroom 20D Minisymposium 14: Cytokinesis
Membrane Organization, Dynamics, Traffic, and Regulation Ballroom 20A Minisymposium 15: Endo-Lysosome Trafficking in Development and Disease
Multicellular Interactions, Tissues, and Development Room 28D Minisymposium 16: Morphogenesis
Organelles and Spatial Organization of the Cell Room 30C Minisymposium 17: New Technologies and Their Application to Probe the Spatial Organization of the Cell
Cytoskeleton, Motility, and Cell Mechanics Ballroom 20C Minisymposium 18: Regulation and Integrated Functions of Actin Cytoskeleton
6:45-8:00 pm ASCB-Gibco Emerging Leader Prize Presentation and E.B. Wilson Ballroom 20BC Medal Presentation and Address by Elaine Fuchs Daily Schedule—Tuesday, December 15
104 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Tuesday, December 15 l Career Center 7:30 am-8:00 pm Learning Center
Stop by any time to check out the job postings.
9:00 am–4:00 pm One-on-One CV Review Drop in and have an experienced ASCB member help you hone the perfect CV. Personalized Career Coaching Drop by to talk one-on-one with life sciences career experts about your personal career trajectory. Immigration Consultation Stop by for a free individual immigration consultation from experts from Getson & Schatz. l Symposium 5: Bending Nature to Our Purposes: Engineering of Cells and Tissues 8:00-9:30 am Ballroom 20BC
Supported by SGI-DNA, A Synthetic Genomics, Inc. Company Chair: Julie Theriot, Stanford University Medical Center
8:00 am S11 CRISPR-Cas genome surveillance: from basic biology to transformative technology. J.A. Doudna1; 1Molecular & Cell Biology and Chemistry, Howard Hughes Medical Institute, Berkeley, CA 8:30 am S12 Giving new life to materials for energy, the environment and medicine. A. Belcher1; 1Biological TUESDAY Engineering, MIT, Cambridge, MA 9:00 am S13 Hydrogels as synthetic extracellular matrices: from tissue engineering to 4D cell biology. K. Anseth1; 1Chemical and Biological Engineering, University of Colorado and HHMI, Boulder, CO l Exhibitor Tech Talk 8:15-9:15 am Theater 1, Learning Center
Sciencell Research Laboratories: Maximize your cell culture: technical tips for primary cells, pluripotent stem cells, and cell analyses Presenter: Jennifer Welser-Alves Level: Intermediate For over a decade, ScienCell Research Laboratories has helped researchers with their cell culture experiments by providing expert advice. We will discuss primary cell culture optimization techniques and why primary cells are necessary for validation of cell line studies and in vivo experiments. In addition, we will examine the importance of selecting the appropriate pluripotent stem cell expansion and differentiation media and illustrate the advantages of using pluripotent stem cells for your research. Lastly, learn how to advance your primary cell research by using our new GeneQuery™ qPCR Arrays kits for gene expression profiling. l ASCB Learning Center (Exhibit Hall) Open 7:30 am-8:00 pm Exhibits open 9:30 am-4:00 pm. l Morning Refreshment Break 9:30-11:30 am
Join us for complimentary coffee and tea while visiting exhibitors and viewing posters.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 105 l Exhibitor Tech Talk 9:30-10:30 am Theater 2, Learning Center
9:30-9:50 am, Reveal Biosciences: Quantitative multiplexed in situ hybridization (ISH) in tissue and cell-based assays Presenter: Claire Weston, PhD Level: Intermediate
In this presentation we will describe a highly sensitive multiplex technique to visualize mRNA and miRNA in tissue sections and cultured cells. The beautiful images that result from this approach can be quantified using ImageDx, our proprietary image analysis software, to provide quantitative data at the single copy level. Learn how we can accelerate your research using a highly sensitive assay to visualize and quantify mRNA and miRNA in your biological samples.
9:50-10:10 am, One World Lab: New technologies to assess quality and compatibility of commercial antibodies from multiple vendors Presenter: Mike Simson Level: Intermediate
Many of the antibodies published in biomedical research suffer from the lack of properly controlled studies into their reliability and reproducibility against specific antigens. Issues with improperly characterized antibodies prevented the replication of data in 47 of 53 preclinical studies. It is estimated that the resulting waste in labor and consumable costs are $350 million annually just for U.S. researchers. To address this crisis, two complimentary technologies have been developed that will create a more structured, accurate antibody system of validation. Bio-Layer Interferometry will be shown to determine Ab concentration, structural integrity, relative KD measurements, and epitope binning. Size exclusion chromatography multi-analyte platform will be shown to determine Ab off-site binding, specific binding, binding to protein complexes, and monomeric forms of proteins.
10:10-10:30 am, EditBIOMED: What can a scientific editing service do for you? Presenter: Melanie Styers Level: Introductory
Today’s scientific research is a global endeavor. Groundbreaking research is conducted in all parts of the world and by scientists of all nationalities. However, English remains the language of science, and the vast majority of scientific and medical journals are published in English. Thus, a lack of proficiency in English presents a significant barrier for acceptance of publications in these journals. EditBIOMED provides affordable scientific editing and proofreading services to help scientists publish their work in English-language journals. Our highly trained editors understand the complexities of the science, and also have an outstanding command of the English language. We correct the English, with an eye for the science, in order to help you publish your research.
l Symposium 6: Going the Distance: Determining Size and Spacing of Biological Structures 9:45-10:45 am Ballroom 20BC
Chair: Shirley Tilghman, Princeton University
9:45 am S14 Mechanisms of mitosis and size control in Xenopus. M. Strzelecka1, R. Heald1; 1Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 10:15 am S15 Turing pattern formation without diffusion. S. Kondo1; 1Frontier Bioscience, Osaka University, Osaka, Japan
106 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l One-Minute, All-Video Elevator Speech Contest 10:00 am-12:00 pm ASCB Booth (721), Learning Center
The premise of the One Minute Elevator Speech Contest is simple: The elevator door closes and you’ve got a trapped audience—a U.S. Senator, your dean, or Taylor Swift. Go for it! Sell your science in 60 seconds! To enter the all-video One-Minute Elevator Speech Contest, make a selfie video of your speech, upload it to YouTube or Vimeo, and then go to www.ascb.org/elevatorspeech to fill out the form linking to your uploaded video. Don’t have a phone or a camera to record your video in San Diego? Come to the Elevator Speech Contest Entry Point at the ASCB Booth Tuesday morning where a camera awaits you. l Table Talk 10:00-11:00 am Roundtable Central Section 1, Learning Center
Transitioning to a Postdoc Position Tracie Gibson, University of Missouri, Columbia l Building a Successful Research Program with Undergraduates—A Roadmap 10:30 am-12:30pm Career Center Theater, Learning Center
Joyce Fernandes, Professor, Miami University This discussion will engage participants in the logistics of beginning and developing a research program, while having simultaneous responsibilities in teaching and service. Choosing projects, recruiting and training students, grant writing, integrating research into teaching labs, are some of the topics that will be explored. l Exhibitor Tech Talk
10:45-11:45 am Theater 1, Learning Center TUESDAY
Wiley: Want people to read your paper? Here’s how to optimize your chances… Presenter: Andrew Moore, Editor-in-Chief Level: Intermediate Many people click on but don’t fully read an article; others don’t even find it. Many sound articles don’t even get to peer review. Which factors influence the success of your manuscript, and at which stage, from submission to publication and final readership recognition? What are editors and reviewers looking for? Did you know that you’re addressing different readerships at different stages of your manuscript’s progress? How do search engines work, and how do editors work with authors to “optimize” their articles for findability and general “noticeability”? How has online reading psychology changed the game for authors? Learn more about these topics and how to write well. Visit the Wiley booth after the session to ask an editor about writing an editorial. l Exhibitor Tech Talk 10:45-11:45 am Theater 2, Learning Center
Tecan: Rapid generation of compound serial dilutions for cell assays using an inkjet based dispensing technology Presenter: Simon Fogarty, Director of Application Sciences Level: Introductory The Tecan D300e Digital Dispenser offers a simple method for rapidly generating compound serial dilutions, and compound combination or synergy experiments while minimizing DMSO concentration in the assay. Using HP’s Direct Digital Dispensing technology, it provides picoliter to microliter non-contact dispensing of liquids directly into the cell plates, saving time, minimizing consumption of valuable samples, and accelerating research. From small molecules in DMSO to biomolecules in surfactant-containing aqueous solutions, this convenient bench-top solution allows rapid delivery of any dose to any well. Requiring almost no set-up time, it uses disposable Dispense heads to minimize dead volumes and virtually eliminates the risk of cross-contamination, offering high quality, low volume dispensing for a wide range of applications
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 107 l Women in Cell Biology (WICB) Committee Awards Presentation/Mentoring Theater 10:45 am-12:00 pm Room 32B
WICB Junior Award for WICB Mid-Career Award for Sandra K. Masur Senior Excellence in Research Excellence in Research Leadership Award
Mihaela (Ela) Serpe Amy S. Gladfelter Angelika Amon Eunice Kennedy Shriver National Dartmouth College Koch Institute for Integrative Cancer Institute of Child Health and Research, Massachusetts Institute Human Development, NIH of Technology
Mentoring Theater: Who Me? I’m Not Biased. Embracing Diversity to Improve Creativity Coordinators: Angela Wandinger-Ness, University of New Mexico, Paula Bubulya, Wright State University, and MariaElena Zavala, California State University, Northridge Organized by the ASCB Women in Cell Biology Committee
l Morning with the Editors: An Open Discussion with Journal Editors 10:45 am-12:00 pm Room 29C
Supported by Cell Biology International, through the International Federation for Cell Biology
Moderator: Bruce Alberts, University of California, San Francisco, and former Editor-in-Chief of Science
Panelists: David Drubin, University of California, Berkeley, and Editor-in-Chief, Molecular Biology of the Cell George Santangelo, Office of Portfolio Analysis, NIH Sergio Schenkman, Universidade Federal de São Paulo, Brazil, and Editor-in-Chief, Cell Biology International Ron Vale, University of California, San Francisco/HHMI Mark Winey, University of Colorado, Boulder, and Chair, ASCB Data Reproducibility Task Force
Following from the very successful interactive session, “An Afternoon with the Editors” that was held during last year’s meeting, this panel discussion with audience input aims to address the following three hot topics associated with the publication of research:
1. Update on The San Francisco Declaration on Research Assessment (DORA). 2. Reproducibility of results. 3. The future of publishing, technology and what scholarly publishing will look like 10 years from now.
Panel members will address questions and comments from the audience.
108 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l Advocacy Toolbox: How to Start and Sustain a Policy Advocacy Group 11:00 am-12:00 pm Room 24A
Science policy advocacy isn’t limited to Washington, DC. Science policy advocacy groups are starting up at institutions all across the United States. Come hear from leaders of these groups and learn how they started their groups. Learn about the do’s and don’ts and get tips on how you can start your own student group.
Kellyann Jones- Anthony J. Koleske Julia Omotade Thomas D. Pollard Charles Easley Jamtgaard Yale University Emory University Yale University Emory University University of Kansas School of Medicine
Organized by the ASCB Public Policy Committee l LGBTQ Diversity Session 11:00 am-12:00 pm Room 24B
Chair: Bruno da Rocha-Azevedo, Federal University of Rio de Janeiro, Brazil
All ASCB members are welcome to join us for one hour of scientific knowledge and career advice / networking for Lesbian, Gay, TUESDAY Bisexual, Transsexual, Queer Cell Biologists and diversity allies. The session includes a scientific presentation of an accomplished LGBTQ scientist and a career conversation between the speaker and the public.
11:00-11:10 am Introduction. Bruno da Rocha-Azevedo, Federal University of Rio de Janeiro, Brazil
11:10-11:40 am Mobilization of the actin cytoskeleton by microbial pathogens. Matthew Welch, University of California, Berkeley, Berkeley, CA 11:40 am–12:00 pm Career advice and open discussion
l Brazil: Opportunities for the Young: Fellowships, Resources, and Interactions 11:00 am-12:00 pm Room 23B
Coordinator: Celia R.S. Garcia, University of São Paulo, Brazil Cell biology research in Brazil: Opportunities for young and senior scientists, Celia R.S. Garcia – University of Sao Paulo, Brazil Support for Young Scientists in the State of Rio de Janeiro, Jerson Lima Silva, Director of Rio de Janeiro State Research Foundation FAPERJ This session will highlight the fast growth of cell biology research in Brazil. The Rio de Janeiro State Research Foundation (FAPERJ) has a variety of programs that support postdoctoral fellows, young and senior investigators, and visiting researchers wishing to spend research time in Brazil. The agencies CNPq, CAPES, and FAPESP also have programs supporting cell biology research internships for Brazilian students in excellent research labs abroad. Organized by the ASCB International Affairs Committee
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 109 l Table Talk 11:00 am-12:00 pm Roundtable Central Section 3, Learning Center
Bringing Research into the Undergraduate Curriculum Sarah Elgin, University of Washington in St. Louis
l Table Talk 11:30 am-12:30 pm Roundtable Central Section 1, Learning Center
Developing Case Studies in Cell Biology Merri Lynn Casem, California State University l Exhibitor Tech Talk 12:00-12:45 pm Theater 2, Learning Center
EMD Millipore: New advances in microfluidic control of cellular microenvironment with uninterrupted imaging allows for highly controllable, long-term, more in vivo-like cell culture studies Presenters: Paul Hung and Kurt Thorn Level: Intermediate A major technical challenge for long-term analysis of cell behavior during in vitro culture is controlling and manipulating microenvironment parameters (temperature, gases, etc.) without moving the culture or impeding visualization. In this workshop, microfluidic design engineer Dr. Paul Hung (EMD Millipore) and video microscopy center Director Prof. Kurt Thorn (UCSF) discuss in detail the latest advancements in microfluidically controlled cell culture and live cell microscopy. The speakers will discuss cell culture considerations, microfluidic design and fabrication requirements, and integrating microfluidic systems with microscopy. The presenters then discuss a range of applications from bacterial biofilm dynamics to tumor metastasis to demonstrate cell shape analysis, individual cell tracking, and manipulation of the microenvironment followed by open discussion and a first look at “next generation” platforms.
l Odd-Numbered Poster Presentations 12:00-1:30 pm Learning Center
l Microsymposium 13: Morphology of the Cytoskeleton Leading to Morphology in Development 12:00-1:10 pm Microsymposia Room 1, Learning Center
Moderators: Alana Gray, Louisiana State University Health - Shreveport; Gary McDowell, Tufts University; and Swaran Nandini, University of Central Florida
12:00 pm Introduction 12:05 pm E85 Association of the RNAi machinery with the zonula adherens regulates growth-related signaling. A. Kourtidis1, J.M. Carr1, I.K. Yan1, T. Patel1, E.A. Thompson1, P.Z. Anastasiadis1; 1Department of Cancer Biology, Mayo Clinic, Jacksonville, FL 12:10 pm E86 Cell shape changes required for brain morphogenesis are mediated by calcium signaling and non- muscle myosin II. S.U. Sahu1, C. Kwas1, M.R. Visetsouk1, R.J. Garde1, J.H. Gutzman1; 1Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 12:15 pm E87 Epithelial cell migration in the intestinal villi depends on actin-driven cell protrusions and mitotic pressure in the crypts. D. Krndija1, E. Hannezo2, S. Richon1, A. Simon1, D.M. Vignjevic1; 1UMR 144, Institut Curie, Paris, France, 2Cavendish Laboratory, University of Cambridge, Cambridge, UK 12:20 pm E88 Muscle-specific ribosome ynthesiss coordinates overall body growth and development in Drosophila by regulating systemic insulin signaling. S.S. Grewal1, A. Ghosh1; 1Biochemistry and Molecular Biology, University of Calgary, Calgary, AB 12:25 pm E89 Mechanics serves as an instructional cue driving heart progenitor cells to undergo a mesenchymal-to-epithelial transition during early heart morphogenesis. T.R. Jackson1, H. Kim1, 110 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting L.A. Davidson1,2,3; 1Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, 2Department of Developmental Biology, University of Pittsburgh, Pittsburgh, PA, 3Department of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA 12:30 pm E90 Mechanical strain guides the formation of global planar axis in ciliated epithelia. Y. Chien1, R. Keller2, C. Kintner1, D. Shook2; 1Molecular Neurobiology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, 2Department of Biology, University of Virginia, Charlottesville, VA 12:35 pm E91 Gut on the chip: extracellular matrix composition and crypt-villus topography dictate the growth and maintenance of intestinal epithelium. M. Verhulsel1, A. Simon2, D. Ferraro1, C. Bureau1, J. Viovy1, S. Descroix1, D.M. Vignjevic2; 1UMR 168, Institut Curie, Paris, France, 2UMR 144, Institut Curie, Paris, France l Microsymposium 14: Actin Cytoskeleton Dynamics 12:00-1:10 pm Microsymposia Room 2, Learning Center
Moderators: Pinar Gurel, National Heart, Blood, and Lung Institute, NIH; andScott Wilkinson, Emory University
12:00 pm Introduction 12:05 pm E92 Sharpin is a novel activator of the Arp2/3 complex. M.H. Khan1,2, S. Salomaa1, A. Augenlicht1, T. Deguchi3, G. Jacquemet4, E. Kremneva5, A. Byron4, M.J. Humphries4, P. Hänninen3, P. Lappalainen5, J. Pouwels1; 1Centre for Biotechnology, University of Turku, Turku, Finland, 2Turku Doctoral Programme of Molecular Medicine, University of Turku, Turku, Finland, 3Laboratory of Biophysics, University of Turku, Turku, Finland, 4Faculty of Life Sciences, University of Manchester, Manchester, UK, 5Institute of Biotechnology, University of Helsinki, Helsinki, Finland 12:10 pm E93 Nuclear actin interactome reveals new functions for actin in the nucleus. T. Viita1, G. Huet1, H. Asan-Liski1, A. Hyrskyluoto1, J. Virtanen1, M. Varjosalo1, M.K. Vartiainen1; 1Institute of Biotechnology, University of Helsinki, Helsinki, Finland 12:15 pm E94 Palladin promotes actin polymerization at pointed ends. R. Gurung1, R. Yadav1, M.R. Beck1; 1Chemistry, Wichita State University, Wichita, KS 12:20 pm E95 Identification of the possible states of organisation of the actin cytoskeleton using high content TUESDAY image screening of a high diversity chemical library. N.S. Bryce1, A. De Laurentiis1,2, T. Failes3, G.M. Arndt3, J.R. Stehn1, E.C. Hardeman1, P.W. Gunning1; 1School of Medical Sciences, UNSW Australia, Sydney, Australia, 2Department of Clinical and Experimental Medicine, University Magna Graecia of Catanzaro, Cantanzaro, Italy, 3ACRF Drug Discovery Centre for Childhood Cancer, Children’s Cancer Institute Australia, Lowy Cancer Research Centre, UNSW Australia, Sydney, Australia 12:25 pm E96 EB1, CLIP-170, and mDia1 trigger ultrafast actin filament polymerization from microtubule plus ends. J.L. Henty-Ridilla1, A. Rankova1, J. Eskin1, B.L. Goode1; 1Biology, Brandeis University, Waltham, MA 12:30 pm E97 Myosin VIII links actin to microtubules during polarized growth. S. Wu1, M. Bezanilla1; 1Biology, University of Massachusetts Amherst, Amherst, MA 12:35 pm E98 Molecular mechanisms of force transmission through linkers of the nucleoskeleton and cytoskeleton. Z. Jahed1, M. Mofrad1, H. Shams1; 1Bioengineering, University of California, Berkeley, CA l Exhibitor In-Booth Presentation 12:00 pm Booth 934
Abcam, Inc: Immunostaining: From sample prep through troubleshooting and beyond Presenter: Thomas Novak, Abcam Scientific Support Specialist
l Afternoon Refreshment Break 12:00-4:00 pm
Join us for ice tea and snacks while visiting exhibitors and viewing posters.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 111 l Exhibitor Tech Talk 12:00-12:45 pm Theater 1, Learning Center
GE Healthcare: Quantitative Western blotting: improving your data quality and reproducibility Presenter: Viola Ruddat, Imaging Sales Specialist Level: Intermediate Western blots have been around for over 30 years and are used in practically every life science laboratory. Equally well known, but often not satisfactorily addressed, are the challenges of reproducibility and the difficulty in obtaining accurate quantitative data and reliable results from Western blot experiments. This talk will describe the new Amersham WB system for SDS PAGE and Western blotting. Detection is based on fluorescence and the methods are standardized with built-in evaluation software. The issues of reproducibility will be explored in more depth and how to obtain higher quality data will be discussed, with a focus on those key factors necessary to create consistent, quantifiable results.
l Navigating the New NIH Biosketch 1:00-1:55 pm Career Center Theater, Learning Center
Sue Biggins, Fred Hutchinson Cancer Research Center; Rebecca Heald, University of California, Berkeley; Sandra Masur, Icahn School of Medicine at Mount Sinai Hospital; and Claire Walczak, Indiana University
This workshop will discuss the mandatory changes that the NIH made to the biosketch format. Learn how to most effectively highlight your work in the new “impact” section and obtain additional tips about tools available to streamline the process. You are welcome to bring your biosketch for feedback from the organizers.
l Exhibitor Tech Talk 1:00-1:45 pm Theater 1, Learning Center
Beckman Coulter Life Sciences: Extracellular vesicle isolation by flow cytometric sorting and characterization by analytical ultra-centrifugation and dynamic light scatter Presenters: Carley Ross and Thomas Ramin Level: Intermediate
The extracellular vesicle (EV) research field has dramatically increased in the last five years. Using a high-speed flow cytometric sorter, EVs may be isolated at high rates such that researchers can differentially separate, isolate, and characterize the EVs for downstream analysis. EVs contaminated with proteins, dye, or antibody aggregates of the same size, but different mass, can be characterized based on these physical properties in the analytical ultra-centrifuge. We demonstrate that the XLA/I Analytical Ultracentrifuge (AUC) effectively separated particles on their sedimentation velocity and clarified issues with dye aggregation vs EV staining. Additionally, the DelsaMax Particle Characterizer allowed for quick analysis of post-sorted populations. The Astrios EQ Cell Sorter was able to sort EVs and AUC provided additional analysis for exosome purity.
112 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l Exhibitor Tech Talk 1:00-1:45 pm Theater 2, Learning Center
Andor: Fast and sensitive camera technologies for microscopy Presenter: Colin Coates Level: Intermediate We will discuss state-of-the-art, ultrasensitive, high speed camera technology applications such as dynamic live cell imaging, single molecule detection, and super-resolution microscopy. sCMOS is a new high-performance imaging technology that can be considered progressive in its ability to simultaneously offer extremely low noise, rapid frame rates, wide dynamic range, high resolution and a large field of view, overcoming many performance trade-offs that are commonly associated with CCD detectors. EMCCDs however, retain the advantage of single photon sensitivity and often remain the best choice for extremely low light imaging. This workshop will discuss and clarify the key sensor characteristics for consideration when selecting the optimum solution for your low light fluorescence microscopy application.
l Exhibitor In-Booth Presentation 1:00-2:00 pm Booth 1235/1237
National Institutes of Health (NIH): National Institute of General Medical Sciences (NIGMS) and Center for Scientific Review (CSR) Presenters: NIGMS Program Directors and CSR Scientific Review Officers Maximizing Investigators’ Research Award (MIRA)
l Microsymposium 15: Membrane Regulation and Signaling 1:25-2:35 pm Microsymposia Room 1, Learning Center TUESDAY Moderators: Brooke Gardner, University of California, Berkeley; and Courtney Schroeder, University of California, San Francisco
1:25 pm Introduction 1:30 pm E99 A tale of deception told in 3D: Golgi modifications by an alphavirus to engineer its exit-pod. R. Sengupta1, E. Mihelc1, S. Angel1, R.J. Kuhn1, J.K. Lanman1; 1Biological Sciences, Purdue University, West Lafayette, IN 1:35 pm E100 Identification of a novel negative regulator of lipophagy and its role in macrophage foam cell formation. T.Y. Nazarko1, S.H. Choi2, A. Glieder3, Y.I. Miller2, S. Subramani1; 1Section of Molecular Biology, UC San Diego, La Jolla, CA, 2Department of Medicine, UC San Diego, La Jolla, CA, 3Institute of Molecular Biotechnology, Graz University of Technology, Graz, Austria 1:40 pm E101 Activated GPCRs exit cilia through ectosome release or BBSome-mediated retrieval. A.R. Nager1, F. Ye1, V. Herranz-Pérez2, J.S. Lee1, J. Manuel Garcia-Verdugo2, M.V. Nachury1; 1Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, 2Laboratorio de Neurobiología Comparada, Instituto Cavanilles, Universitat de València, Valencia, Spain 1:45 pm E102 Intercellular communication pathways are hijacked by bacterial pathogens during cell-to-cell spread. R. Lamason1, M.D. Welch1; 1MCB, UC Berkeley, Berkeley, CA 1:50 pm E103 Endocytic membrane-associated septins are required for macropinosome maturation and fusion with lysosomes. L. Dolat1, E.T. Spiliotis1; 1Biology, Drexel University, Philadelphia, PA 1:55 pm E104 β-arrestin drives MAP kinase signaling after dissociating from its activating GPCR through kinetic arrest of clathrin-mediated endocytosis. K. Eichel1, D. Jullié1, M.E. Von Zastrow1,2; 1Psychiatry, University of California, San Francisco, San Francisco, CA, 2Cellular Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 2:00 pm E105 Autophagy preserves the functional capacity of hematopoietic stem cells during aging. T.T. Ho1, M.R. Warr1, J. Debnath2, E. Passegue1; 1Department of Medicine, University of California, San Francisco, San Francisco, CA, 2Department of Pathology, University of California, San Francisco, San Francisco, CA
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 113 l Microsymposium 16: Cell Biology of Genetic Information 1:25-2:35 pm Microsymposia Room 2, Learning Center
Moderators: Abigail Buchwalter, Salk Institute of Biological Studies; and Melissa Pamula, Rockefeller University
1:25 pm Introduction 1:30 pm E106 Cell-to-cell transfer of mRNA via membrane nanotubes. G. Haimovich1, J.E. Gerst1, R.H. Singer2,3; 1Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel,2 Anatomy Structural Biology, Albert Einstein College of Medicine , Bronx, New York, NY, 3Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine , Bronx, New York, NY 1:35 pm E107 Association of heterochromatin with the nuclear envelope drives nuclear stiffness. S.M. Schreiner1, Y. Zhao2, P. Koo2, S. Mochrie2,3, M.C. King1; 1Cell Biology, Yale School of Medicine, New Haven, CT, 2Physics, Yale University, New Haven, CT, 3Applied Physics, Yale University, New Haven, CT 1:40 pm E108 Constricted cell migration drives lamin segregation, repair factor demixing, and DNA damage. J. Irianto1, C.R. Pfeifer1, A. Athirasala1, I.L. Ivanovska1, R.A. Greenberg2, D.E. Discher1; 1Biophysical Engineering Labs, University of Pennsylvania, Philadelphia, PA, 2Department of Cancer Biology, Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, PA 1:45 pm E109 Structural insights into the recognition of the histone tails by karyopherins. M. Soniat1,2, Y. Chook1,2; 1Pharmacology, University of Texas Southwestern, Dallas, TX, 2Biophysics, University of Texas Southwestern, Dallas, TX 1:50 pm E110 Protein turnover and dynamics spanning seconds to months in non-dividing cells. B.H. Toyama1, M.W. Hetzer1; 1Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 1:55 pm E111 Slicing activity of the Argonaute CSR-1 tunes expression of germline genes to control embryonic cell division and germline development. A. Gerson-Gurwitz1, S. Wang1, R.A. Green1, K. Oegema1, A.B. Desai1; 1Dep. of Cellular Molecular Medicine, Ludwig Cancer Research, San Diego, CA 2:00 pm E112 NuMA regulates the mobility of 53BP1 in the cell nucleus and its accumulation at DNA damage sites. P. Vidi1, J. Liu2, M. Gray3, L. Parker4, J. Irudayaraj2, S.A. Lelievre3; 1Cancer Biology, Wake Forest School of Medicine, Winston-Salem, NC, 2Agricultural and Biological Engineering, Purdue University, West Lafayette, IN,3 Basic Medical Sciences, Purdue University, West Lafayette, IN, 4Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Saint Paul, MN
l Table Talk 1:30-2:30 pm Roundtable Central Section 1, Learning Center
Crafting a Research Statement That Is Undergraduate Research Friendly Karen Resendes, Westminster University; and Michael Palladino, Monmouth University
l Even-Numbered Poster Presentations 1:30-3:00 pm Learning Center
114 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l Career Panel: Industry and Sales 2:00-2:55 pm Career Center Theater, Learning Center
These panelists will give a short talk about their career experiences and then will address individual questions from the audience. Di Mo, PhD, is a Global Channel Partner Sales Specialist with Illumina. She obtained a PhD in Integrative Molecular Biology from the University of Pittsburgh School of Medicine in 2012. After her graduate training, she completed a postdoctoral fellowship at the National Institutes of Health in Bethesda, MD. Mo developed an interest in sales and marketing and subsequently accepted a position as a marketing manager at a biotech company based in Baltimore, MD. She moved to San Diego with her husband to join Illumina as an Inside Sales Representative in 2013. While at Illumina, she supported several districts with consistent track records of exceeding territory sales objectives by generating new business and growing existing accounts. In 2015, she transitioned to Global Channel Partner team as a Sales Specialist within Illumina to support distributors in APAC and China. She manages channel partner trainings, compliance programs, business reviews, and creates sales programs/activities to help drive sales in global distributor regions.
Jonah Cool, PhD, is a Scientist in the Therapeutics group of Organovo, Inc. His primary roles focus on the development of regenerative medicine applications using Organovo’s proprietary bioprinting platform. Prior to Organovo, his training was completed at Duke University Medical Center and the Salk Institute for Biological Science. His work examined the role of remodeling vasculature on organ formation and progenitor cell differentiation.
Prithwish Pal, PhD, Senior Product Manager, joined Illumina in 2014, after stints both in academia and industry, which provided him with a unique perspective on research and driving products toward success. As part of his PhD in Biophysics, he studied structural mechanisms of membrane protein function using fluorescence-based techniques, which he followed up with a postdoc at Klaus Hahn’s lab at University of North Carolina, Chapel Hill, developing tools for spatio-temporal imaging in live cells. Subsequently, he transitioned to the industry, initially as a scientist developing nanopore-based DNA sequencing technologies, followed by various commercial roles, including business development for an ion-channel recording device, marketing for cancer diagnostic kits, business analysis for a personalized cancer therapy center, and market development for a lab-sharing marketplace startup. In his latest role as Senior Product Manager at Illumina, he manages product development and marketing for a cloud-based platform for storage, analysis, and sharing of genomics data. TUESDAY Organized by the Committee for Postdocs and Students (COMPASS) l Exhibitor Tech Talk 2:00-2:45 pm Theater 1, Learning Center
Thermo Fisher Scientific: Automated protein detection - the future of Westerns Presenter: Kevin Lowitz, Senior Product Manager, Protein & Cellular Analysis Level: Intermediate Western blotting remains one of the most ubiquitous techniques used by life science researchers around the world to advance their understanding of various disease areas such as cancer and immunology. Learn how new product innovations are providing more flexibility and better results than ever before to address a time-consuming and error-prone method for protein analysis. We will discuss the new iBind™ Flex Western System to highlight: • Sequential lateral flow technology for hands-free immunodetection • Optimized platform for improved performance and potential cost savings • Broad compatibility across the entire Western workflow l Exhibitor Tech Talk 2:00-2:45 pm Theater 2, Learning Center
Essential Pharmaceuticals: Variables in FBS impact cell performance, reproducibility, and relevant experimental results Presenter: Adam Elhofy, PhD, CSO Level: Introductory Fetal bovine serum (FBS) has been used in cell culture for decades. Even though it introduces the greatest variability into cell culture,
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 115 surprisingly FBS is not intensely scrutinized as a reagent. The use of FBS in cell culture applications will be examined, as its use may go against the basic principles of scientific experimentation in that the variables are not controlled for, it contains unknowns, and results from experiments using FBS in cell culture potentially cannot be replicated.
l Microsymposium 17: Spindle Assembly and Chromosome Dynamics 2:50-4:00 pm Microsymposia Room 1, Learning Center
Moderators: Christopher Maxwell, University of British Columbia, Canada; and Melissa Pamula, Rockefeller University
2:50 pm Introduction 2:55 pm E113 DNA replication-timing maps reveal dynamic chromatin domains in the developing zebrafish embryo. J.C. Siefert1,2, C.L. Sansam1,2, A. Koren3; 1Cell Cycle & Cancer Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK,2 Department of Cell Biology, University of Oklahoma Health Science Center, Oklahoma City, OK, 3Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 3:00 pm E114 Altered epidermal development and growth control, without perturbed homeostasis or tumorigenesis, in the presence of centrosome amplification. A. Kulukian1, A. Holland2, B.D. Vitre2, D.W. Cleveland2, E. Fuchs1; 1Mammalian Cell Biology and Development, Rockefeller University, New York, NY, 2Ludwig Institute for Cancer Research; Dept of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 3:05 pm E115 Operational characteristics of the kinetochore-based signaling reactions of the spindle assembly checkpoint. P. Aravamudhan1, R. Chen2, J.H. Sim2, A.P. Joglekar2; 1Biophysics, University of Michigan, Ann Arbor, MI, 2Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 3:10 pm E116 Probing the physical inputs controlling the spindle assembly checkpoint. J.A. Kuhn1,2, S. Dumont1,3; 1Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, 2Tetrad Graduate Program, University of California, San Francisco, San Francisco, CA, 3Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 3:15 pm E117 Differential chromatin states can regulate chromosome length scaling. A. Ladouceur1, L. Smith1, J.G. Lawrimore1, K.S. Bloom1, P.S. Maddox1; 1Biology, University of North Carolina, Chapel Hill, NC 3:20 pm E118 Cooperation between kinesin motors promotes spindle symmetry and chromosome organization in oocytes. S.J. Radford1, A.M. Go1,2, K.S. McKim1,2; 1Waksman Institute, Rutgers University, Piscataway, NJ, 2Department of Genetics, Rutgers University, Piscataway, NJ 3:25 pm E119 Timing and pattern of release of chromosome cohesion differs between species with monocentric and holocentric chromosomes. K.D. Felt1, S. Thibault1, A.M. Martens1, L.V. Paliulis1; 1Biology Department, Bucknell University, Lewisburg, PA
l Microsymposium 18: Cell Mechanics and Adhesion 2:50-4:00 pm Microsymposia Room 2, Learning Center
Moderators: Bruno Da Rocha-Azevedo, Federal University of Rio de Janeiro; and Cristian Suarez, University of Chicago
2:50 pm Introduction 2:55 pm E120 AMPK: A novel link between E-cadherin force transmission and cell metabolism. J.L. Bays1, C. Heidema1, W. Hacker1, K.A. DeMali1; 1Department of Biochemistry, University of Iowa, Iowa City, IA 3:00 pm E121 Force regulation of talin unfolding by a molecular clutch defines cell rigidity sensing and transduction. A. Elosegui-Artola1, R. Oria1,2, Y. Chen3,4, A. Kosmalska1,2, C. Pérez-González1,2, N. Castro1, C. Zhu3,4,5, X. Trepat1,2,6, P. Roca-Cusachs1,2; 1Institute for Bioengineering of Catalonia, Barcelona, Spain, 2University of Barcelona, Barcelona, Spain, 3Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA,4 Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA,5 Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA,6 Institució Catalana de Recerca i Estudis Avançats , Barcelona, Spain 3:05 pm E122 T cell membrane adhesion and organization on engineered surrogate antigen presenting cells.
116 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting P. Dillard1,2, F. Pi1, L. Limozin2, K. Sengupta1; 1AMU-CNRS UMR 7325, CINaM, Marseille, France, 2INSERM UMR 1067 AMU-CNRS UMR 7333, LAI, Marseille, France 3:10 pm E123 Mechano-sensitivity of nascent adhesions on soft substrates revealed by fluorescence fluctuation analysis and traction microscopy. S.J. Han1, A. Bachir2, A.R. Horwitz2, G. Danuser1; 1Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, 2Department of Cell Biology, University of Virginia, Charlottesville, VA 3:15 pm E124 Protein tyrosine kinases control local pinching involved in adhesion-dependent mechanosensing. B. Yang1, Z. Lieu1, H. Wolfenson2, F.M. Hameed 1, A.D. Bershadsky1,3, M.P. Sheetz1,2; 1Mechanobiology Institute, National University of Singapore, Singapore,2 Columbia University, Department of Biological Sciences, New York, NY, 3Weizmann Institute of Science, Department of Molecular Cell Biology, Rehovot, Israel 3:20 pm E125 Catch-bond adhesion of filopodia: involvement of myosin II and formins. N.O. Alieva1, A.K. Efremov1, S. Hu1, M. Natarajan1, A.D. Bershadsky1,2; 1National University of Singapore, Mechanobiology Institute, Singapore,2 Weizmann Institute of Science, Rehovot, Israel 3:25 pm E126 Targeting adhesive molecules to prevent protective cancer cell niches. J.L. Young1, S. Klar2, J.P. Spatz1,3; 1New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Stuttgart, Germany, 2Biomedical Sciences, Reutlingen University, Reutlingen, Germany, 3Biophysical Chemistry, Heidelberg University, Heidelberg, Germany
l Celldance Videos, Elevator Speech Contest Finals, and Comic Contest Awards 3:00-4:00 pm Theater 1, Learning Center
Celldance Studios (a.k.a. the ASCB Public Information Committee) has once again bankrolled three ASCB member labs with $1,000 each to make a “Tell Your Own Cell Story” video, featuring live cell imaging and aimed at biology educators, the news media, and the general public. NIH Director Francis Collins called last year’s Celldance videos “microscopic blockbusters.” Come see this year’s biggest little pictures and meet the filmmakers.
We will also announce the winners of the One-Minute All-Video Elevator Speech Contest, which takes the video selfie, ASCB TUESDAY members, and public outreach to new heights. The winners of the Comic Contest run by ASCB’s Committee for Postdocs and Graduate Students (COMPASS) will also be revealed. l Exhibitor Tech Talk 3:00-4:00 pm Theater 2, Learning Center
Essen BioScience: Novel kinetic live cell imaging assays for T cell killing of tumor cells Presenter: Daniel Appledorn Level: Advanced Cancer immunotherapy offers the potential for eradication of tumor cells and the prevention of cancer recurrence. In this study, we describe live cell imaging assays designed to quantify T cell killing of tumor cells in co-culture. PBMCs or isolated CD8+ T cells were added to tumor cells in combination with activators and a non-perturbing caspase-3/7 reagent in 96 or 384-well plates. Phase and 2-color images were captured using IncuCyte ZOOM™. Tumor cells and apoptotic cells were quantified using image analysis. These data demonstrate that a live cell imaging approach can discern the full time course and specificity of T cell killing, without lifting cells, using Ab labels or radioisotopes. HD images and time-lapse movies facilitate a clear understanding and compelling verification of the underlying biology.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 117 l Research in Cell Biology in France: Opportunities and Tips 3:00-4:00 pm Room 23B
Presenters: Thierry Galli, Deputy Director of Multi-Organization-Thematic-Institute of Cell Biology, Development and Evolution and Corinne Albiges-Rizo, President, French Society for Cell Biology This session will highlight current cell biology research, education, and scientific publication activities in France. It will also discuss the potential opportunities for career development, particularly for young researchers, and for international collaboration on fundamental and translational research. The event is specifically designed for those who plan to explore employment opportunities (PhD theses, postdoc, researcher position, and young group leaders, sabbatical) and/or establish collaborative efforts in France. Organized by the ASCB International Affairs Committee l Career Panel: Research Development and Grant Administration 3:00-4:00 pm Career Center Theater, Learning Center
These panelists will give a short talk about their career experiences and then will address individual questions from the audience. Alexandra Ainsztein, PhD, is a Program Director in the Division of Cell Biology and Biophysics, National Institute of General Medical Sciences, NIH. She manages research grants related to membrane trafficking, the molecular mechanisms and dynamics of the secretory pathway and organelle biogenesis, as well as all aspects of the cell biology of the nucleus. Before joining the Institute, she served as a scientific review officer in the NIH Center for Scientific Review. Ainsztein earned a BA in biochemistry from Brandeis University, a PhD in biochemistry and molecular biology from the University of Florida, and then did postdoctoral research in cell biology at Johns Hopkins University, the University of Edinburgh, and NIH’s National Institute of Child Health and Human Development. Sharon E.R. Franks, PhD, is Director of the Research Proposal Development Service (RPDS) at University of California, San Diego, where she works with faculty throughout campus to facilitate the development of major interdisciplinary funding proposals. Since the establishment of RPDS in 2009, Sharon has provided guidance, coordination, and in-depth support for more than 80 research proposals to federal, state, and private sponsors including NSF, NIH, DOE, DoD, USDA, CIRM, and the Packard, Moore, and Sloan Foundations, among others. The majority of these proposals were multimillion-dollar, multi-investigator, multi-institution, interdisciplinary proposals with budgets totaling more than $900 million. Among her other responsibilities are management of the campus’ limited submissions process, implementation of professional development programs for early career investigators, strategic planning for the identification and dissemination of funding opportunities, and coordination of a $2 million California neurotechnology seed grant program. Prior to launching RPDS, Frankis created and led institutionalization of a proposal development initiative that helped faculty and other researchers at UC San Diego’s Scripps Institution of Oceanography address NSF’s broader impacts review criterion. Sharon earned a BA in Earth Sciences from Dartmouth College and a PhD in Oceanography from Oregon State University. Organized by the ASCB Committee for Postdocs and Students (COMPASS)
l Science Discussion Tables 3:00-3:50 pm Roundtable Central Section 3, Learning Center
Whether you’re a student, postdoc, or PI, ASCB will again offer special networking opportunities with senior scientists and peers. Select your interest area and bring your questions to the ASCB Learning Center in the San Diego Convention Center.
Table No. Presenter Topic 1 Mina Bissell What regulates P53 activity in physiological conditions. Minain normal breast? 2 Daniela Cimini Aneuploidy 3 Elizabeth Marincola Open Access 4 Kathleen Green Cell adhesion and signaling 5 Rebecca Heald Cell division and biological size control 6 Denise Montell Cell adhesion, cell migration, cell survival
118 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 7 Samara Reck-Peterson Cytoskeletal molecular motors 8 Forest Rohwer Microbiomes and Holobionts 9 Gia Voeltz Organelle biogenesis
l ASCB Business Meeting 3:00-4:00 pm ASCB Booth (721) Theater, Learning Center
Join leaders of the ASCB to learn about the state of the Society and the passing of the gavel from Shirley Tilghman to Peter Walter. l Large-Scale Data Workshop: Image Analysis in Quantitative Microscopy 3:00-6:25 pm Room 31B Supported by Hamamatsu Corporation Presenters: Mark Bray and Anne E. Carpenter, Broad Institute of Harvard and MIT
Mark Bray Anne E. Carpenter Broad Institute of Harvard and MIT Broad Institute of Harvard and MIT TUESDAY The overview is open to all attendees. Preregistration is required for the hands-on portion of the workshop. 3:00-4:00 pm Didactic Overview. Open to all ASCB attendees. 4:00-6:25pm Hands-on workshop. Limited to preregisterd 40 participants. Image-based experiments in cultured cells are powerful, generating information-rich, high-content data for diverse biological applications. The didactic overview will introduce biologists to the sorts of phenotypes that can be quantified in images and basic concepts of image analysis. The hands-on workshop will instruct biologists in the use of CellProfiler, an open-source, freely downloadable software package designed for automated phenotypic image analysis, at both large- and small-scale. We will also demonstrate CellProfiler Analyst, which uses machine learning to score phenotypes where phenotypic differences between samples are subtle or even invisible by eye. Preregistered participants are encouraged to submit sample images from their assays as part of the demonstration (contact [email protected] for details).
If you are registered for the hands-on workshop, be sure to remember to bring your laptop with the following software downloaded prior to the session: CellProfiler: Available from http://cellprofiler.org/download.shtml CellProfiler Analyst: Available from http://cellprofiler.org/downloadCPA.shtml A folder of translocation assay images: Available from http://cellprofiler.org/linked_files/TranslocationData.zip. Open the zip file and place the folder “TranslocationData” in a location with read/write access (e.g., your desktop). Make sure that Java is installed for your OS: http://java.com/en/download/manual.jsp
If this session is full but people do now show up, we will let people in at the door on a first-come, first-served basis.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 119 l Exhibitor In-Booth Presentation 3:00-4:00 pm Booth 1235/1237
National Institutes of Health (NIH): National Institute of General Medical Sciences (NIGMS) and Center for Scientific Review (CSR) Presenters: Michael Sesma, Chief, Postdoctoral Training Branch, Division of Training, Workforce Development, and Diversity, NIGMS, and Robert Horowits, Scientific Review Officer, NIGMS NIH Postdoctoral Fellowships and K99 Awards l Subgroup P: The Cellular and Molecular Basis of Invasive Metastatic Cancer 3:00-6:30 pm Room 32B
Organizers: Mark A. McNiven, Mayo Clinic; Laura M. Machesky, Beatson Institute, Cancer Research UK; and Alissa M. Weaver, Vanderbilt University
This subgroup will focus on understanding the important and widespread process of how tumor cells actively interact with and remodel the surrounding microenvironment through a combination of migration, matrix degradation, physical forces, and signal transduction during the metastatic process. The program will feature experts in matrix biology, tumor microenvironment, cytoskeletal and organelle dynamics, in situ live cell imaging, mouse and other genetic model systems, and human pathology to provide a state-of-the-art update on new findings and technologies to both understand and curtail metastatic disease.
Presentations: 3:00–3:10 pm Introduction. Alissa M. Weaver, Vanderbilt University; Mark A. McNiven, Mayo Clinic; Laura Machesky, Beatson Institute, Glasgow, UK 3:10–3:35 pm Actin cytoskeletal control of migration and metastasis of pancreatic cancer. Laura Machesky and Amelie Juin, Beatson Institute, Glasgow, UK 3:35–4:00 pm Mechanical and structural cues within the 3D microenvironment in metastatic cell migration. Cynthia Reinhart-King, Cornell University 4:00–4:25 pm The role of the physical microenvironment in cancer cell invasion. Denis Wirtz, Johns Hopkins University 4:25–4:50 pm When cells collide: contact inhibition as a migratory cue. Brian Stramer, King’s College London, UK 4:50–5:15 pm Stromal-tumor cell interactions in matrix remodeling. Gina L. Razidlo and Mark A. McNiven, Mayo Clinic 5:15–5:40 pm Localized energy sensing through LKB1 and AMPK recruits mitochondria to the leading edge. Alan Howe, University of Vermont 5:40–6:05 pm Wnt5a regulates protein depalmitoylation to promote polarized cell movement. Eric Witze, University of Pennsylvania 6:05–6:30 pm Stromal regulation of tumor aggressiveness. Erik Sahai, Francis Crick Institute, London, UK
120 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting l Education Initiative Forum 3:15-3:45 pm Room 24B
Using Visual Networks to Map the Systems Thinking Literature
Tammy M. Long Michigan State University
So, what exactly, is systems thinking? And, how can I know if my students are using it? National reports have advocated inclusion of systems learning at all levels in STEM education, but characterizing what it means to be a “systems thinker” has proven difficult. As an alternative to the standard literature review, our team has adapted an Information Sciences approach that harnesses the power of large bibliographic databases to identify and visualize key communities, publications, and themes that will inform the development of a systems thinking framework for teaching and learning in undergraduate biology.
l Poster Removal 3:30-4:00 pm Learning Center
All posters must be removed from the ASCB Learning Center before it closes permanently at 4:00 pm. No access to the ASCB Learning Center will be permitted after 4:00 pm due to safety concerns. No exceptions. TUESDAY l Applications of Cell Biology in the Real World Minisymposium 13: Applications of Cell Biology 1 4:00-6:25 pm Room 29C
Co-Chairs: Lisa Belmont, Genentech, Inc.; and Craig Blackstone, National Institute of Neurological Disorders and Stroke/NIH
4:00 pm Introduction 4:05 pm M110 *A synthetic transcriptional program for preventing proteostasis collapse derived from the ancient function of Heat Shock Factor 1. V. Denic1; 1Molecular and Cellular Biology, Harvard University, Cambridge, MA 4:25 pm M111 Strategies for targeting SMARCA4 mutant cancer. L.D. Belmont1; 1Discovery Oncology, Genentech, Inc., South San Francisco, CA 4:45 pm M112 Mining cellular heterogeneity for mechanistic insights in phenotypic profiling and drug discovery. A.H. Gough1,2, T. Shun2, D.L. Taylor1,2,3, M. Schurdak1,2,3; 1Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA,2 Drug Discovery Institute, University of Pittsburgh, Pittsburgh, PA,3 Cancer Institute, University of Pittsburgh, Pittsburgh, PA 5:05 pm M113 Development of cellular morphology-based separation system for three-dimensional culture. H. Matsui1, M. Tamura2, S. Sugiura2, R. Kato3, M. Yanagisawa4, T. Kanamori2; 1Faculty of Medical Sciences, University of Tsukuba, Tsukuba, Japan, 2National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan, 3Nagoya University, Nagoya, Japan, 4Engineering System Co., Ltd., Matsumoto, Japan 5:25 pm M114 Applying m‘TORC’1 to prolong vision in Retinitis pigmentosa. A. Venkatesh1, S. Ma1, C. Punzo1; 1Ophthalmology and Gene Therapy Center, University of Massachusetts Medical School, Worcester, MA 5:45 pm M115 Using tardigrades to investigate mechanisms and applications of desiccation tolerance. T.C.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 121 Boothby1, H. Tapia2, A.H. Brozena3, D.E. Koshland2, B. Goldstein1; 1Department of Biology, University of North Carolina, Chapel Hill, NC, 2Department of Molecular & Cell Biology, University of California, Berkeley, Berkeley, CA, 3Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 6:05 pm M116 Leveraging the cell biology of metabolic enzymes to uncover new insights into orphan genetic diseases. R.M. Broyer1, C. Noree1, E. Monfort1, J.E. Wilhelm1; 1Cell and Developmental Biology, UC San Diego, La Jolla, CA 6:12 pm M117 Reconstituting chemically induced dimerization system as a potential artificial chemoattractant sensing mechanism in giant liposomes. S. Razavi1,2, L. Tianzhi2, D.N. Robinson2,3, T. Inoue1,2,4; 1Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, 2Cell Biology, Center for Cell Dynamics, Johns Hopkins School of Medicine, Baltimore, MD, 3Chemical and Biomolecular Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, 4Precursory Research for Embryonic Science and Technology Investigator, Tokyo, Japan 6:19 pm M118 Cardiac-inducing RNAs direct the differentiation of stem cells into cardiomyocytes for heart repair. A. Kochegarov1, M.S. Neal1, A. Davis1, L. Mitchell1, N. Scarcelli1, G. Vaughn1, L.F. Lemanski1, H. Fetters1; 1Biological and Environmental Sciences, Texas AM University, Commerce, TX
*Vlad Denic is the recipient of the Early Career Life Scientist Award. l Cell Cycle and Cell Division Minisymposium 14: Cytokinesis 4:00-6:25 pm Ballroom 20D
Co-Chairs: Michael Glotzer, University of Chicago; David Morgan, University of California, San Francisco; and Jody Rosenblatt, University of Utah, Huntsman Cancer Institute
4:00 pm Introduction 4:05 pm M119 Nanoscale architecture of the actomyosin cortex during cell division. B. Truong Quang1,2, P. Chugh1,2, M. Smith1,2, G. Salbreux3, E.K. Paluch1,2; 1UCL, MRC Laboratory for Molecular Cell Biology, London, UK, 2UCL, Institute for the Physics of Living Systems, London, UK, 344 Lincoln’s Inn Fields, The Francis Crick Institute, London, UK 4:25 pm M120 RhoA activation is sufficient to induce cleavage furrow formation in metaphase and anaphase: an optogenetic analysis of cytokinesis. E. Wagner1, M. Glotzer1; 1Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 4:45 pm M121 The cell cortex is an excitable medium. W.M. Bement1, A.L. Miller2, M. Leda3, A. Goryachev3, G. von Dassow4; 1Laboratory of Cell and Molecular Biology, University of Wisconsin-Madison, Madison, WI, 2Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, 3Centre for Synthetic and Systems Biology, University of Edinburgh, Edinburgh, UK, 4Oregon Institute for Marine Biology, Charleston, OR 5:05 pm M122 A dynamic steady state within ESCRT-III polymers at the cytokinetic abscission site. B. Mierzwa*1,2, N. Chiaruttini*3, J. König2,4, I. Poser5, A.A. Hyman5, T. Müller-Reichert2,4, A. Roux3, D.W. Gerlich1,2; 1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna, Austria, 2Marine Biological Laboratory, Woods Hole, MA, 3Department of Biochemistry, University of Geneva, Geneva, Switzerland, 4Medical Theoretical Center, Dresden University of Technology, Dresden, Germany, 5Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany *Co-first authors 5:25 pm M123 Fak-Src signaling pathway controls the timing of abscission by decelerating Plk1 degradation and subsequent recruitment of Cep55 at mid-body. S.A. Kamranvar 1, D.K. Gupta1, S. Johansson1; 1Department of Medical Biochemistry and Microbiology, Biomedical Center, Uppsala University, Uppsala, Sweden 5:45 pm M124 Loss of p120-catenin induces cancer multinucleation and polyploidy through cytokinesis failure. R. van de Ven1, J. de Groot1, D. Park2, R. van Domselaar1, D. de Jong3, K. Szuhai3, E. Sahai2, P.J. van Diest1, M.W. Hetzer4, P.W. Derksen1, E. van der Wall5; 1Pathology, UMC Utrecht, Utrecht, Netherlands, 2Tumour Cell Biology Laboratory, Cancer Research UK London Research Institute,
122 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting London, UK, 3Molecular Cell Biology, Leiden University Medical Center, Leiden, Netherlands, 4Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA,5 Internal Medicine, UMC Utrecht, Utrecht, Netherlands 6:05 pm M125 Tension generated by cytokinesis remodels cell-cell junctions and recruits Anillin. T.R. Arnold1, T. Higashi1, K.M. Dinshaw1, R.E. Stephenson1, A.L. Miller1; 1Molecular Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI
l Membrane Organization, Dynamics, Traffic, and Regulation Minisymposium 15: Endo-Lysosome Trafficking in Development and Disease 4:00-6:25 pm Ballroom 20A
Supported by Traffic, Wiley Co-Chairs: Satyajit Mayor, National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India; and Xiaochen Wang, National Institute of Biological Sciences, Beijing, China
4:00 pm Introduction 4:05 pm M126 The protein architecture of the yeast endocytic machinery analyzed by FRET. M. Skruzny1,2, G. Malengo1,2, V. Sourjik1,2; 1Max Planck Institute for Terrestrial Microbiology, Marburg, Germany, 2LOEWE Center for Synthetic Microbiology (SYNMIKRO), Marburg, Germany 4:25 pm M127 Mechanisms of endocytosis: shape and size dependence. M.S. Magon1,2,3, G. Battaglia2,3; 1BBSRC London Interdisciplinary Biosciences DTP, University College London, London, UK, 2Department of Chemistry, University College London, London, UK, 3MRC/UCL Centre for Medical Molecular Virology, University College London, London, UK 4:45 pm M128 Differential control of death-receptor endocytosis and apoptosis by dynamins. C.R. Reis1, S.L. Schmid1; 1Cell Biology, UT Southwestern Medical Center, Dallas, TX 5:05 pm M129 Polarized endosome dynamics by spindle asymmetry during asymmetric cell division. E. Derivery1, C. Seum1, A. Daeden1, S. Loubéry1, L. Holtzer1, F. Julicher2, M. Gonzalez-Gaitan1; 1Biochemistry, University of Geneva, Geneva, Switzerland, 2Max Planck Institute for the Physics of Complex TUESDAY Systems, Dresden, Germany 5:25 pm M130 LMA-1 maintains lysosome integrity and normal adult life span in C. elegans. Y. Li1, W. Zou1, B. Chen1, X. Wang2, Y. Sun1, C. Yang2, X. Wang1; 1National Institute of Biological Sciences, Beijing, China, 2State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing, China 5:45 pm M131 Mechanisms of mTORC1-independent lysosome biogenesis. Y. Li1, M. Xu1, X. Ding2, Z. Song3, X. Huang1, Y. Jian1, G. Tang2, C. Yang3, Y. Di1, X. Liu1, K. Liu1, T. LI1, Y. Wang1, X. Hao2,3, C. Yang1; 1Center for Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China, 2Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China, 3The Key Laboratory of Chemistry for Natural Product of Guizhou Province and CAS, Guiyang, China 6:05 pm M132 Real time analysis of Clathrin and Dynamin-independent endocytic vesicle formation: Role of actin nucleators and BAR domain proteins. M. Sathe1, G. Muthukrishnan1, M. Thattai1, S. Mayor1; 1National Center for Biological Sciences, Bangalore, India 6:12 pm M133 Mdm1/Snx13 is a novel inter-organelle membrane contact site tethering protein. M. Henne1, L. Zhu2, Z. Balogi2, C. Stefan2, J. Pleiss2, S. Emr2; 1Cell Biology, UT Southwestern, Dallas, TX, 2Weill Institute, Cornell University, Ithaca, NY 6:19 pm M134 Lysophagy: novel selective autophagy eliminating damaged organelles and suppressing diseases. M. Hamasaki1, H. Teranishi1, I. Maejima1, T. Yoshimori1; 1Department of Genetics Graduate School of Medicine, Osaka University, Osaka, Japan
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 123 l Multicellular Interactions, Tissues, and Development Minisymposium 16: Morphogenesis 4:00-6:25 pm Room 28D
Co-Chairs: Carl-Philipp Heisenberg, Institute of Science and Technology Austria (IST Austria); and Sean Megason, Harvard Medical School
4:00 pm Introduction 4:05 pm M135 Surface cell expansion triggers radial cell intercalations in zebrafish gastrulation. C. Heisenberg1; 1IST Austria, Klosterneuburg, Austria 4:25 pm M136 Local and tissue scale forces drive oriented junction growth during tissue extension. C. Collinet1, M. Rauzi1, P. Lenne1, T. Lecuit1; 1CNRS, IBDM Institute de Biologie du Développement de Marseille, Marseille, France 4:45 pm M137 Compliance sensing by actomyosin self-organization coordinates epithelial tension and tissue shape. S. Chanet1, C. Miller2, C. Vasquez1, B. Ermentrout3, L.A. Davidson2, A. Martin1; 1Biology, MIT, Cambridge, MA, 2Bioengineering, Pittsburgh University, Pittsburgh, PA, 3Mathematics, Pittsburgh University, Pittsburgh, PA 5:05 pm M138 Pressure in developmental size control. I.A. Swinburne1, K.R. Mosaliganti1, A. Green1, T. Hiscock1, L. Mahadevan2,3, S.G. Megason1; 1Department of Systems Biology, Harvard University, Boston, MA, 2Department of Physics, Harvard University, Cambridge, MA, 3School of Engineering and Applied Science, Harvard University, Cambridge, MA 5:25 pm M139 Stochastic fluctuations in oxidative stress signaling induce anisotropies in adhesion and cytoskeletal organization to influence cell behavior and spatial patterning in aDrosophila epithelium. M. Narasimha1, S. Muliyil1,2, S. Saravanan1; 1Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, India, 2Sir William Dunn School of Pathology, University of Oxford, Oxford, UK 5:45 pm M140 aMOTIV microscopy: mechanical characterization of the in vitro and in vivo tissue microenvironment. J.R. Staunton1, B.H. Blehm1, A. Devine1, K. Tanner1; 1National Cancer Institute, National Institutes of Health, Bethesda, MD 6:05 pm M141 Actomyosin force generation directs hydra regeneration. K. Keren1, A. Livshitz1, L. Zerbib1, E. Braun1; 1Physics, Technion- Israel Institute of Technology, Haifa, Israel 6:12 pm M142 Conserved roles for cytoskeletal components in determining laterality. G.S. McDowell1,2, J. Lemire1,2, J. Pare1,2, M. Levin1,2; 1Biology Department, Tufts University, Medford, MA,2 Center for Regenerative and Developmental Biology, Tufts University, Medford, MA 6:19 pm M143 Development plain and simple: using cartography to analyze forces driving morphogenesis. S.J. Streichan1, I. Heemskerk1, M.F. Lefebvre2, E. Wieschaus2, B. Shraiman1; 1Kavli Institute for Theoretical Physics, University of California Santa Barbara, Santa Barbara, CA, 2Molecular Biology, Princeton University , Princeton, NJ l Organelles and Spatial Organization of the Cell Minisymposium 17: New Technologies and Their Application to Probe the Spatial Organization of the Cell 4:00-6:25 pm Room 30C
Co-Chairs: Kathryn Lilley, University of Cambridge, UK; and Alice Ting, Massachusetts Institute of Technology
4:00 pm Introduction 4:05 pm M144 Mapping the subcellular proteome and determining dynamic subcellular rearrangements using quantitative mass spectrometry. K.S. Lilley1, A. Christoforou1, C.M. Mulvey1, L.M. Breckels1, L. Gatto1, A. Geladaki1, T. Hurrell1, D.J. Nightingale1, H. Zhou1, A. Martinez-Arias2; Department of Biochemistry, University of Cambridge, Cambridge, UK, 2Department of Genetics, University of Cambridge, Cambridge, UK 4:25 pm M145 Discovery and characterization of novel synaptic and mitochondrial proteins via peroxidase- mediated live cell proteomic mapping. A.Y. Ting1; 1Chemistry, Massachusetts Institute of Technology, Cambridge, MA 4:45 pm M146 Spectrally resolved super-resolution microscopy. Z. Zhang1,2, S. Kenny1, M. Hauser1, W. Li1, K. Xu1,2; 1Department of Chemistry, University of California, Berkeley, CA, 2Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA
124 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 5:05 pm M147 Exploring the native molecular architecture of organelles with in situ cryo-electron tomography. B.D. Engel1, M. Schaffer1, S. Albert1, S. Asano1, L. Kuhn Cuellar1, S. Pfeffer1, J.M. Plitzko1, W. Baumeister1; 1Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany 5:25 pm M148 The Balbiani body in Xenopus forms by amyloid-like aggregation. E. Boke1, R. Lemaitre2, S. Alberti2, A.A. Hyman2, D.N. Drechsel2, T.J. Mitchison1; 1Department of Systems Biology, Harvard Medical School, Boston, MA, 2Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany 5:45 pm M149 The nucleolus as an active multiphase droplet. M. Feric1, N. Vaidya1, T.M. Richardson1, C.P. Brangwynne1; 1Department of Chemical & Biological Engineering, Princeton University, Princeton, NJ 6:05 pm M150 Genetically encoded nanoparticles reveal mechanisms that control cellular biophysics. K.J. Kennedy1, J. Guttierez1, I.V. Surovtsev2, C. Renou1, C. Jacobs-Wagner2, L.J. Holt1; 1Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, 2Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 6:12 pm M151 Proteomic clues to cell organization. M. Wühr1,2, T. Güttler2, L. Peshkin1, G.C. McAlister2, M. Sonnett1,2, K. Ishihara1, A.C. Groen1, M. Presler1, B.K. Erickson2, T.J. Mitchison1, S.P. Gygi2, M.W. Kirschner1; 1Department of Systems Biology, Harvard Medical School, Boston, MA, 2Department of Cell Biology, Harvard Medical School, Boston, MA 6:19 pm M152 Optogenetic control of molecular motors and organelle distributions in cells. L. Duan1, D. Che1, K. Zhang2, Q. Ong1, S. Guo1, B. Cui1; 1Chemistry, Stanford University, Stanford, CA, 2Biochemistry, University of Illinois Urbana-Champaign, Champaign, IL l Cytoskeleton, Motility, and Cell Mechanics Minisymposium 18: Regulation and Integrated Functions of Actin Cytoskeleton 4:00-6:25 pm Ballroom 20C
Co-Chairs: William Brieher, University of Illinois, Urbana-Champaign; and Guillaume Charras, University College London TUESDAY 4:00 pm Introduction 4:05 pm M153 Structural investigation of cooperative actin disassembly. V. Tang1, A. Nadkarni1, W.M. Brieher1; 1Cell and Developmental Biology, University of Illinois, Urbana-Champaign, Urbana, IL 4:25 pm M154 apCAM adhesion sites are mechanically isolated from retrograde actin flow by local Arp 2/3 complex-dependent actin assembly during neurite growth. K.B. Buck1, A.W. Schaefer1, V.T. Schoonderwoert1, M.S. Creamer1, E.R. Dufresne2, P. Forscher1; 1Molecular Cell and Developmental Biology, Yale University, New Haven, CT, 2Mechanical Engineering, Yale University, New Haven, CT 4:45 pm M155 Cellular control of cortical actin nucleation. M. Bovellan1, A. Yonis1, Y. Romeo2, M. Biro3, A. Boden4, P. Chugh4,5, M. Vaghela1, M. Fritzsche1, D. Moulding6, A. Jegou7, A.J. Thrasher6, G. Romet-Lemonne7, E.K. Paluch4,5, P.P. Roux2, G. Charras1; 1London Centre for Nanotechnology, University College London, London, UK, 2IRIC, Universite de Montreal, Montreal, Canada, 3Centenary Institute of Cancer Medicine and Cell Biology, University of Sydney, Sydney, Australia, 4Molecular Cell Biology and Genetics, Max Planck Institute , Dresden, Germany, 5MRC-LMCB, University College London, London, UK, 6Institute of Child Health, University College London, London, UK, 7LEBS, CNRS, Gif sur Yvette, France 5:05 pm M156 Chromosome transport during starfish oocyte meiosis: a model for 3D contraction generated by actin filament dynamics. P. Bun1, S. Dmitrieff1, M. Mori2, F. Nedelec1, P. Lénárt1; 1Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany, 2Genome Information Research Center, Osaka University, Osaka, Japan 5:25 pm M157 Pulsatile contractions are an intrinsic property of myosin IIa in adherent cells. M.A. Baird1, R.S. Fischer1, A. Wang 1, R.S. Adelstein1, C.M. Waterman1; 1NHLBI, National Institutes of Health, Bethesda, MD 5:45 pm M158 From molecules to meshes: generating tension in an actin cortex. M.B. Smith1, P. Chugh1, D. Cassani1, G. Salbreux2, E.K. Paluch1; 1LMCB, UCL, London, England, 2Theoretical Physics of Biology, The Francis Crick Institute, London, England 6:05 pm M159 Protrusive waves guide 3D cell migration along nanofibers. C. Guetta-Terrier1, H. Long2,3, P. Monzo1, J. Zhu4, Z. Yue5,6, P. Wang5,6, S.Y. Chew2,3, A. Mogilner7, B. Ladoux1,8, N.C. Gauthier1; 1Mechanobiology Institute, National University of Singapore, Singapore,2 School of Chemical &
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 125 Biomedical Engineering, Nanyang Technological University, Singapore, 3Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, 4Cellular and Molecular Physiology, Yale University, New Haven, CT, 5Cardiovascular Research Institute, National University Health System, Singapore, 6Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 7Courant Institute and Department of Biology, New York University, New York, NY, 8Institut Jacques Monod, CNRS UMR 7592 Université Paris Diderot, Paris, France 6:12 pm M160 Emergence of an apical epithelial cell surface in vivo. J. Sedzinski1, E. Hannezo2, F. Tu1, M. Biro3, J.B. Wallingford1; 1Molecular Biosciences, University of Texas, Austin TX, 2Cavendish Laboratory, Department of Physics, University of Cambridge, Cambridge, UK, 3University of Sydney, Sydney, Australia 6:19 pm M161 Filament spacing in actin bundles is an architectural feature that drives protein sorting. J.D. Winkelman1, C. Suarez1, A.J. Harker2, G.M. Hocky3, J.R. Christensen1, A.N. Morganthaler1, J.R. Bartles4, D.R. Kovar1; 1Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, 2Biochemistry and Molecular Biology, University of Chicago, Chicago, IL, 3Chemistry, University of Chicago, Chicago, IL, 4Feinberg School of Medicine, Northwestern University, Chicago, IL
l ASCB-Gibco Emerging Leader Prize Presentation and E.B. Wilson Medal Presentation and Address 6:45-8:00 pm Ballroom 20BC
ASCB-Gibco Emerging Leader Prize supported by Thermo Fisher Cash prizes of $5,000 will be presented to the top three of 10 finalists for excellence in independent research.
Clifford Brangwynne Ahmet Yildiz Meng Wang Assistant Professor, Assistant Professor, University of Assistant Professor, Baylor College Princeton University California, Berkeley of Medicine
Finalists: Nels Elde, Assistant Professor, University of Utah Guangshuo Ou, Associate Professor, Tsinghua University Melissa Gardner, Assistant Professor, University of Minnesota Antonina Roll-Mecak, Investigator, NIH National Dmitri Kudryashov, Assistant Professor, Ohio State University Institute of Neurological Disorders and Stroke Kelly Monk, Assistant Professor, Washington University Hari Shroff, Chief, NIH National Institute of Biomedical Imaging and Bioengineering
E.B. Wilson Medal Presentation and Address
Elaine Fuchs The Rockefeller University
A12 Skin stem cells: where they come from, how they make tissues and who controls their decisions. E. Fuchs1,2; 1Mammalian Cell Biology & Development, The Rockefeller University, New York, NY, 2Howard Hughes Medical Institute
126 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Wednesday December 16, 2015
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 127 Notes
128 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Daily Schedule—Wednesday, December 16 7:30-11:30 am Registration Open Registration Area
8:30-10:55 am Cytoskeleton, Motility, and Cell Mechanics Minisymposium 19: A Tribute to Alan Hall: Rho GTPase Signaling Ballroom 20C
Applications of Cell Biology in the Real World Minisymposium 20: Applications of Cell Biology 2 Room 29C
Cell Cycle and Cell Division Minisymposium 21: Cell Cycle Regulation Room 31B
Signaling and Differentiation Minisymposium 22: Mammalian Cell Signaling Room 32B
Cytoskeleton, Motility, and Cell Mechanics Minisymposium 23: Motility and Cytoskeleton of Microbes Ballroom 20D
Membrane Organization, Dynamics, Traffic, and Regulation Minisymposium 24: New Insights Into Secretory Trafficking Mechanisms Ballroom 20A
Organelles and Spatial Organization of the Cell Minisymposium 25: Organelle Homeostasis and Turnover Room 30C
Multicellular Interactions, Tissues, and Development Minisymposium 26: Tissue Biology Room 28D
11:15 am-12:15 pm Symposium 7 Ballroom 20BC Beyond the Five Senses: Detection of Magnetic and Electric Fields
12:15 pm Meeting Ends
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 129 l Cytoskeleton, Motility, and Cell Mechanics Minisymposium 19: A Tribute to Alan Hall: Rho GTPase Signaling 8:30-10:55 am Ballroom 20C
Co-Chairs: William Brieher, University of Illinois, Urbana-Champaign ; and Jodi Nunnari, University of California, Davis, and Editor-in-Chief of the Journal for Cell Biology
8:30 am Introduction and Tribute to Alan Hall. Jodi Nunnari, University of California, Davis, and Editor-in- Chief of the Journal for Cell Biology 8:35 am M162 P-cadherin/β-PIX/Cdc42 promotes collective cell migration through increase in the anisotropy and magnitude of mechanical forces. C. Gauthier-Rouviere1, C. Plutoni1, E. Bazellierres2, M. Le Borgne-Rochet1, F. Comunale1, A. Brugués2, D. Planchon1, N.S. Morin1, S. Bodin1, X. Trepat2; 1CRBM, CNRS, Montpellier, France, 2IBEC, Barcelona, Spain 8:55 am M163 The small GTPase Rac3 is essential for invadopodia maturation and function in breast cancer cells. S.K. Donnelly1,2, J. Bravo Cordero1,2, J.S. Condeelis1,2, L. Hodgson1,2; 1Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY, 2Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY 9:15 am M164 Patterning Rho signaling at the epithelial Zonula Adherens: a tale of feedback loops. R. Priya1, G.A. Gomez1, S. Budnar1, S. Verma1, N.A. Hamilton1, A.S. Yap1; 1Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia 9:35 am M165 Spontaneous vs. light-induced symmetry breaking – characterizing the relation of mechanical traction forces and morphological events during cell migration. K. Hennig1, O. Destaing2, C. Albiges-Rizo2, M. Balland1; 1Materials, Optics and Instrumental Techniques for the Life Sciences (MOTIV), Laboratory of Interdisciplinary Physics, Grenoble, France, 2Differentiation and Cell Transformation, Institute Albert Bonniot Inserm U823, Grenoble, France 9:55 am M166 Stress fibers store contractile energy to resist changes in cell shape. P.W. Oakes1, E. Wagner2, C.A. Brand3, D. Probst3, M. Linke3, U.S. Schwarz3, M. Glotzer2, M.L. Gardel1; 1Institute for Biophysical Dynamics, James Franck Institute and Department of Physics, University of Chicago, Chicago, IL, 2Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, 3Institute for Theoretical Physics and BioQuant, Heidelberg University, Heidelberg, Germany 10:15 am M167 RhoA activity cycling promotes dynamic cytoskeletal contractions during epithelial invagination. F.M. Mason1, S. Xie1, M. Tworoger1, A. Martin1; 1Biology, Massachusetts Institute of Technology, Cambridge, MA 10:35 am M168 Roles of guanine nucleotide exchange factors in regulating collective cell migration. A. Zaritsky1, Y. Tseng2, M.A. Rabadán2, M. Overholtzer2, G. Danuser1, A. Hall2; 1Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX, 2Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY l Applications of Cell Biology in the Real World Minisymposium 20: Applications of Cell Biology 2 8:30-10:55 am Room 29C
Co-Chairs: Lisa Belmont, Genentech, Inc.; and Craig Blackstone, National Institute of Neurological Disorders and Stroke, NIH
8:30 am Introduction 8:35 am M169 Increased spatiotemporal resolution reveals highly dynamic, tubular lattices in the peripheral endoplasmic reticulum. J. Nixon-Abell1,2, C.J. Obara3, A.V. Weigel3, D. Li4, W.R. Legant4, K. Harvey2, E. Betzig4, J. Lippincott-Schwartz3, C.D. Blackstone1; 1Cell Neurology Section, National Institute of Neurological Disorders and Stroke, Bethesda, MD, 2Department of Pharmacology, UCL School of Pharmacy, London, UK, 3Section on Organelle Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD, 4Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 8:55 am M170 Kinesin-1 defects lead to altered axonal transport in the important hippocampal to basal forebrain memory circuit in living intact mouse brain. C.S. Medina1, O. Biris2, F. Chaves1, A.J. Zimmerman1, T. Falzone3, R.E. Jacobs4, E.L. Bearer1; 1Pathology, University of New Mexico Health Sciences
130 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Center, Albuquerque, NM, 2Division of Engineering, Brown University, Providence, RI, 3IBCN- Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina,4 Beckman Institute, California Institute of Technology, Pasadena, CA 9:15 am M171 Poly(ADP-Ribosylation) regulates axon regeneration. A.B. Byrne1,2, Y. Sekine2,3, R.D. McWhirter4, S.M. Strittmatter2,3, D.M. Miller III4, M. Hammarlund1,2; 1Genetics, Yale University, New Haven, CT, 2CNNR, Yale University, New Haven, CT, 3Neurology, Yale University, New Haven, CT, 4Cell and Developmental Biology, Vanderbilt University, Nashville, TN 9:35 am M172 Dynamics of thrombus formation in mouse testicular surface vein by new collaborative analysis with live-imaging in vivo and following TEM observation. A. Sawaguchi1, S. Nishimura2,3,4; 1Anatomy, University of Miyazaki, Miyazaki, Japan, 2Cardiovascular Medicine, The University of Tokyo, Tokyo, Japan, 3Translational Systems Biology and Medicine Initiative, The University of Tokyo, Tokyo, Japan, 4Center for Molecular Medicine, Jichi Medical University, Tochigi, Japan 9:55 am M173 Microscopic detection of vulnerable sites exposed on cell-bound HIV to inform better vaccine design. M. Mengistu1, G.K. Lewis1, A.L. DeVico1; 1Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD 10:15 am M174 Neighbor-killing via the Type-VI secretion system enables high-efficiency, cross-species acquisition of antibiotic resistance genes in competent Acinetobacter bacteria. R.M. Cooper1, L. Tsimring1, J. Hasty1; 1Biocircuits Institute, UC San Diego, San Diego, CA 10:35 am M175 The retromer complex regulates exosomal APP at the Drosophila neuromuscular junction. R.B. Walsh1, M.J. Zunitch1, A.N. Becalska1, J. Gittzus1, A.A. Rodal1; 1Department of Biology, Brandeis University, Waltham, MA 10:42 am M176 Gamma-secretase inhibitors (GSIs) and modulators (GSMs) induce distinct conformational changes in the active sites of gamma-secretase and signal peptide peptidase (SPP). N. Gertsik1, D. Chau2, Y. Li3; 1Biochemistry and Structural Biology, Weill Cornell Medical College, New York, NY, 2Pharmacology, Weill Cornell Medical College, New York, NY, 3Pharmacology, Memorial Sloan Kettering Cancer Center, New York, NY 10:49 am M177 Directive nanoscale cues for regenerative neural cell systems. V.M. Ayres1, V.M. Tiryaki2, I. Ahmed3, D.I. Shreiber3; 1Electronic Biological Nanostructures Laboratory, Michigan State University, East Lansing, MI, 2Computer Engineering, Siirt University, Siirt, Turkey, 3Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ l Cell Cycle and Cell Division Minisymposium 21: Cell Cycle Regulation 8:30-10:55 am Room 31B
Co-Chairs: David Morgan, University of California, San Francisco; and Jody Rosenblatt, University of Utah WEDNESDAY 8:30 am Introduction 8:35 am M178 Mechanisms of mitotic regulation by the APC/C. D. Lu1, J.R. Girard1, A. Mizrak1, D.O. Morgan1; 1Physiology, University of California, San Francisco, San Francisco, CA 8:55 am M179 Mechanical stretch triggers rapid epithelial cell division through the stretch-activated channel Piezo1. J. Lindblom1, P.D. Loftus1, K. Edes1, M.J. Redd1, J. Rosenblatt1; 1Oncological Science, Huntsman Cancer Institute, Salt Lake City, UT 9:15 am M180 Lattice light-sheet microscopy of dividing cells in culture and in live zebrafish embryos with high spatiotemporal resolution demonstrates similar membrane and cell volume dynamics. S. Upadhyayula1, F. Aguet1, R. Gaudin1, E. Coccuci1, K. He1, B. Chen2, K.R. Mosaliganti3, W.R. Legant2, T. Liu2, E. Marino1, G. Danuser4, S.G. Megason3, E. Betzig2, T. Kirchhausen1; 1Cell Biology, Harvard Medical School, Boston, MA, 2Howard Hughes Medical Institute , Janelia Research Campus, Ashburn, VA, 3Systems Biology, Harvard Medical School, Boston, MA, 4Cell Biology, UT Southwestern, Dallas, TX 9:35 am M181 Direct visualization of nuclear envelope rupture. N. Wesolowska1, P. Lénárt1, M. Mori2; 1Cell Biology and Biophysics, European Molecular Biology Laboratory, Heidelberg, Germany, 2Osaka University, Osaka, Japan 9:55 am M182 Sphingolipids activate an ER stress surveillance (ERSU) pathway that monitors the proper inheritance of functional endoplasmic reticulum (ER) during the yeast cell cycle. M. Niwa1, F. Yagisawa1, F. Pina-Nunez1, J.T. Chao1, A.B. Tam1; 1Molecular Biology, UCSD, La Jolla, CA 10:15 am M183 Spatial regulation of Greatwall by Cdk1 and PP2A-Tws in the cell cycle. P. Wang1,2, M. Larouche1,2,
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 131 H. Mehsen1,2, K. Normandin2, D. Kachaner1,2, G. Emery2,3, V. Archambault1,2; 1Department of Biochemistry and Molecular Medicine, Université de Montréal, Montréal, QC, 2Institute for Research in Immunology and Cancer, Montréal, QC, 3Department of Pathology and Cell Biology, Université de Montréal, Montréal, QC 10:35 am M184 Bistability of a coupled Aurora B kinase-phosphatase system in cell division. A.V. Zaytsev1, D. Segura-Pena2, E.R. Ballister2, A. Calderon2, A.M. Mayo2, R. Stamatov2, M. Godzi1, L. Peterson3, B.E. Black4, F.I. Ataullakhanov5,6,7, M.A. Lampson2, E.L. Grishchuk1; 1Department of Physiology, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, 2Department of Biology, University of Pennsylvania, Philadelphia, PA, 3Department of Biology and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA,4 Department of Biochemistry and Biophysics, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, 5Department of Physics, Moscow State University, Moscow, Russia, 6Center for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of Sciences, Moscow, Russia, 7Federal Research and Clinical Centre of Pediatric Hematology, Oncology, Moscow, Russia l Signaling and Differentiation Minisymposium 22: Mammalian Cell Signaling 8:30-10:55 am Room 32B
Co-Chairs: Markus Covert, Stanford University; and Dana Pe’er, Columbia University
8:30 am Introduction 8:35 am M185 High resolution ordering of single cells along developmental trajectories with branches. D. Pe’er1; 1Dept of Biological Sciences, Columbia University, New York, NY 8:55 am M186 Paracrine communication maximizes cellular response fidelity in wound signaling. L.N. Handly1, A. Pilko1, R. Wollman1,2,3; 1Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, 2Cell and Developmental Biology, University of California, San Diego, La Jolla, CA, 3San Diego Center for Systems Biology, La Jolla, CA 9:15 am M187 Causes and consequences of variation in p53 signaling dynamics in tissues and tumors. J. Stewart- Ornstein1, G. Lahav1; 1Systems Biology, Harvard Medical School, Boston, MA 9:35 am M188 A circadian code for fat cell differentiation. Z. Bahrami-Nejad1, M.L. Zhao1, K. Tkach1, S. von Schie1, M.N. Teruel1; 1Chemical and Systems Biology, Stanford University, Stanford, CA 9:55 am M189 Modulation of macrophage inflammatory NF-κB signaling by intracellular Cryptococcus neoformans. J.B. Hayes1, L.E. Heusinkveld1, R. Leander2, W. Ding2, E.E. McClelland1, D.E. Nelson1; 1Biology, Middle Tennessee State University, Murfreesboro, TN, 2Mathematics, Middle Tennessee State University, Murfreesboro, TN 10:15 am M190 Chromatin modifies the transmission of TNF-induced NF-κB signals in single cells. V.C. Wong1, A.K. Chavali2, R.E. Lee3,4,5, W. Mothes6, S. Gaudet3,4, K. Miller-Jensen1,2; 1Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, 2Department of Biomedical Engineering, Yale University, New Haven, CT, 3Department of Genetics, Harvard Medical School, Boston, MA, 4Department of Cancer Biology and Center for Cancer Systems Biology, Dana-Farber Cancer Institute, Boston, MA, 5Department of Computational and Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA,6 Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 10:35 am M191 High-sensitivity measurements of multiple kinase activities in live single cells. M. Covert1, S. Regot1, J. Hughey1, B. Bajar1, S. Carrasco1; 1Bioengineering, Stanford University, Stanford, CA l Cytoskeleton, Motility, and Cell Mechanics Minisymposium 23: Motility and Cytoskeleton of Microbes 8:30-10:55 am Ballroom 20D
Co-Chairs: Guillaume Charras, University College London; and Makoto Miyata, Osaka City University
8:30 am Introduction 8:35 am M192 Gliding mechanism of Mycoplasma, the smallest bacteria. M. Miyata1; 1Department of Biology, Graduate School of Science, Osaka City University, Osaka, Japan 8:55 am M193 Mechanisms of an acid-actuated protein lancet. J.K. Polka1,2, M.D. Vahey3, D.A. Fletcher3, J.M. 132 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Kollman4, T.J. Mitchison1, P.A. Silver1,2; 1Systems Biology, Harvard Medical School, Boston, MA, 2Wyss Institute for Biologically Inspired Engineering, Boston, MA,3 Bioengineering, University of California, Berkeley, Berkeley, CA, 4Biochemistry, University of Washington, Seattle, WA 9:15 am M194 Integrated systems biology underlying the final steps of bacterial cell growth. E.R. Rojas1, K.C. Huang2, J.A. Theriot1; 1Biochemistry, Stanford University, Stanford, CA, 2Bioengineering, Stanford University, Stanford, CA 9:35 am M195 IFT-independent translocation of the +TIP protein EB1 in Chlamydomonas flagella. J.A. Harris1, Y. Liu2, P. Yang2, P. Kner3, K.F. Lechtreck1; 1Cellular Biology, University of Georgia, Athens, GA, 2Biological Sciences, Marquette University, Milwaukee, WI,3 Engineering, University of Georgia, Athens, GA 9:55 am M196 Evolutionary retention of two actin nucleation promoting factors, WAVE and WASP, predicts amoeboid motility in the amphibian chytrid fungus. L. Fritz-Laylin1, S. Lord1, R.D. Mullins1; 1Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 10:15 am M197 The torsinA homolog tsin is required for the multicellular development of Dictyostelium discoideum. C.A. Saunders1, J.R. Erickson1, B.M. Woolums1, H. Bauer1, M.A. Titus1, G. Luxton1; 1Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 10:22 am M198 A Gα stimulated Ras/Rap switch regulates Dictyostelium chemotaxis. J. Lacal1, Y. Liu2, D.M. Veltman3, I. Keizer-Gunnink2, F. Fusetti4, P.J. van Haastert2, R.A. Firtel1, A. Kortholt2; 1Division of Biological Sciences, University of California, San Diego, CA, 2Department of Cell Biochemistry, University of Groningen, Groningen, Netherlands, 3Laboratory of Molecular Biology, Medical Research Council, Cambridge, UK, 4Department of Biochemistry and Netherlands Proteomics Centre, University of Groningen, Groningen, Netherlands 10:29 am M199 Mechanism of actin filament assembly by the Vibrio virulence factors VopF and VopL. T.A. Burke1, E. Kerkhoff2, M.K. Rosen3, R. Dominguez4, D.R. Kovar1,5; 1Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL, 2Neurology, University Hospital Regensburg, Regensburg, Germany, 3Biophysics, Biochemistry, Green Center for Systems Biology, University of Texas Southwestern Medical Center, Dallas, TX, 4Physiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 5Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 10:35 am M200 Latrunculin-resistant F-actin and cleavage furrows without myosin II in Chlamydomonas. M. Onishi1, F.R. Cross2, J.R. Pringle1; 1Department of Genetics, Stanford University School of Medicine, Stanford, CA, 2The Rockefeller University, New York, NY 10:42 am M201 MSL8, A mechanosensitive ion channel that protects cells from developmentally imposed osmotic shock. E.S. Haswell1, E.S. Hamilton1, G. Maksaev1, G.S. Jensen1; 1Biology, Washington University in Saint Louis, Saint Louis, MO 10:49 am M202 FtsZ minirings curvature is the opposite of tubulin rings. M. Housman1, M. Osawa1, H.P. Erickson1; 1Cell Biology, Duke University, Durham, NC WEDNESDAY l Membrane Organization, Dynamics, Traffic, and Regulation Minisymposium 24: New Insights Into Secretory Trafficking Mechanisms 8:30-10:55 am Ballroom 20A
Co-Chairs: Adam Linstedt, Carnegie Mellon University; and Xiaochen Wang, National Institute of Biological Sciences, Beijing, China
8:30 am Introduction 8:35 am M203 Visualizing the large procollagen I carrying COPII vesicles by super resolution fluorescence microscopy. L. Yuan1, A. Gorur1, S. Baba1, S. Kenny2, K. Xu2, R.W. Schekman1; 1MCB, University of California, Berkeley, Berkeley, CA, 2Chemistry, University of California, Berkeley, Berkeley, CA 8:55 am M204 Recruitment of ERGIC membranes by TANGO1 and their fusion with the endoplasmic reticulum is required for export of the bulky cargo Collagen VII. I. Raote1,2, A.J. Santos1,2, M. Scarpa1,2, N. Brouwers1,2, V. Malhotra1,2; 1Cell and Developmental Biology, Centre for Genomic Regulation (CRG), Barcelona, Spain, 2Universitat Pompeu Fabra (UPF), Barcelona, Spain 9:15 am M205 COPI selectively drives maturation of the early Golgi. E. Papanikou1, K.J. Day1, J. Austin II1, B.S. Glick1; 1Molecular Genetics & Cell Biology, University of Chicago, Chicago, IL 9:35 am M206 Golgin Tether-tSNARE interaction is required for Golgi stacking. I. Lee1, J.E. Rothman1; 1Shanghai Institute for Advanced Immunochemical Studies, Shanghaitech University, Shanghai, China
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 133 9:55 am M207 The molecular organization of the exocyst determined by live cell imaging. A. Picco1,2, O. Gallego3, I. Irastorza4, T. Specht2, D. Devos4, M. Kaksonen1,2; 1Department of Biochemistry, University of Geneva, Geneva, Switzerland, 2Cell Biology and Biophysics, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany, 3Institute for Research in Biomedicine, Barcelona, Spain, 4Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-CSIC, Sevilla, Spain 10:15 am M208 Functional interactions among Sec17/alpha-SNAP, SM proteins, tethers, and SNAREs in membrane fusion in vitro and in vivo. A.J. Merz1, A. Guitierrez1, B. Lobingier1, D.P. Nickerson1,2, R. Plemel1, M.L. Schwartz1, M. Zick3; 1Biochemistry, University of Washington, Seattle, WA, 2Biology, California State University, San Bernadino, San Bernadino, CA, 3Biochemistry, Geisel School of Medicine, Hanover, NH 10:35 am M209 Fluorescence based analysis of atlastin crossover and fusion kinetics. J. Winsor1, T.H. Lee1; 1Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 10:42 am M210 A novel imaging method for quantitative localization of Golgi proteins at nanometer resolution. H. Tie1, D. Mahajan1, C. Li2, L. Lu1; 1School of Biological Sciences, Nanyang Technological University, Singapore, Singapore, 2Bioinformatics Institute, Singapore, Singapore 10:49 am M211 Quality control of GPI-anchored proteins at the plasma membrane. P. Satpute-krishnan1, B.S. Park1,2, J. Lippincott-Schwartz1; 1Cell Biology and Metabolism Program, NICHD, National Institutes of Health, Bethesda, MD, 2Thomas Jefferson High School for Science and Technology, Alexandria, VA l Organelles and Spatial Organization of the Cell Minisymposium 25: Organelle Homeostasis and Turnover 8:30-10:55 am Room 30C
Co-Chairs: Alexander Van Der Bliek, University of California, Los Angeles; and Xinnan Wang, Stanford University
8:30 am Introduction 8:35 am M212 Dynamic remodeling of the magnetosome membrane is triggered by the initiation of biomineralization. E. Cornejo1, P. Subramanian2, G.J. Jensen2, A. Komeili1; 1Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, CA, 2Department of Biology, California Institute of Technology, Pasadena, CA 8:55 am M213 The SND proteins target SRP-independent substrates to the endoplasmic reticulum. N. Aviram1, T. Ast1, S. Haßdenteufel2, E.A. Costa3, E.C. Arakel4, S. Schorr2, S.G. Chuartzman1, C.H. Jan3, B. Schwappach4, R. Zimmermann2, J.S. Weissman3, M. Schuldiner1; 1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel,2 Department of Medical Biochemistry and Molecular Biology, Saarland University, Homburg, Germany, 3Department of Cellular and Molecular Pharmacology, University of California, San Francisco, and Howard Hughes Medical Institute, San Francisco, CA,4 Department of Molecular Biology, University Medical Center Göttingen, Göttingen, Germany 9:15 am M214 Endogenous parkin preserves dopaminergic substantia nigral neurons following mitochondrial DNA mutagenic stress. A.M. Pickrell1, S.R. Kennedy2, C. Huang1, A. Ordureau3, D.P. Sideris1, J. Hoekstra2, J.W. Harper3, R.J. Youle1; 1National Institutes of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, 2Department of Pathology, University of Washington, Seattle, WA,3 Department of Cell Biology, Harvard Medical School, Boston, MA 9:35 am M215 Pathogenic LRRK2 impairs miro degradation and mitochondrial transport in a pathway also present in sporadic Parkinson’s. C. Hsieh1, A. Shaltouki1, X. Wang1; 1Neurosurgery, Stanford University, Palo Alto, CA 9:55 am M216 Axonal autophagosomes recruit dynein for retrograde transport through fusion with late endosomes. X. Cheng1, B. Zhou1, M. Lin1, Z. Sheng1; 1NINDS, NIH, Bethesda, MD 10:15 am M217 Role of Fis1 in mitochondrial fission and stress. A.M. Van Der Bliek1, R. Youle2; 1Biological Chemistry, David Geffen School of Medicine at UCLA, Los Angeles, CA, 2National Institutes of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 10:35 am M218 Synchronous regulation of nuclear and mitochondrial translation during mitochondrial biogenesis. M. Couvillion1, S. Churchman1; 1Department of Genetics, Harvard Medical School, Boston, MA
134 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 10:42 am M219 A cellular molecular timer measures synaptic vesicle use and prevents the participation of aged vesicles in synaptic transmission. S. Truckenbrodt1, A. Viplav2, A. Denker3, A. Vogts4, E.F. Fornasiero1, S.O. Rizzoli1; 1Department for Neuro- and Sensory Physiology, University of Göttingen Medical School, Göttingen, Germany,2 Cells in Motion Cluster of Excellence, University of Münster, Münster, Germany, 3Hubrecht Institute, Utrecht, Netherlands, 4Leibniz-Institute for Baltic Sea Research, Rostock, Germany 10:49 am M220 Dissecting the mechanisms of liquid ot solid phase transition of the ALS protein FUS. A. Patel1, L. Jawerth1, T.M. Franzmann1, R. Wheeler1, A.A. Hyman1; 1Hyman Lab, Max-Planck-Institute of Cell Biology and Genetics, Dresden, Germany l Multicellular Interactions, Tissues, and Development Minisymposium 26: Tissue Biology 8:30-10:55 am Room 28D
Co-Chairs: Iswar Hariharan, University of California, Berkeley; and Carien Niessen, University of Cologne, Germany
8:30 am Introduction 8:35 am M221 Mechanisms underlying the age-related decline in the regenerative capacity of Drosophila imaginal discs. R.E. Harris1, L. Setiawan1, J. Saul1, I.K. Hariharan1; 1Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 8:55 am M222 Polarization of junctions, cytoskeleton and signal receptors in multi-layered epithelia. C.M. Niessen1,2,3, M. Rübsam1,2,3, S. Vorhagen1,2,3, F. Tellkamp1,2,3, J. Xia1,2,3, J. Nafizi1,2,3, B. Boggetti1,2,3; 1Department of Dermatology, University of Cologne, Cologne, Germany, 2Center for Molecular Medicine Cologne, University of Cologne, Cologne, Germany, 3Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany 9:15 am M223 *A novel, noncanonical BMP pathway modulates synapse maturation at the Drosophila neuromuscular junction. M.J. Sulkowski1, T. Han1, C.M. Ott1, E.M. Verheyen2, J. Lippincott- Schwartz1, M. Serpe1; 1NICHD, NIH, Bethesda, MD, 2Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC 9:35 am M224 Discovery of a hormone requirement for cell growth allows visualization of growth dynamics in a proliferating epithelial tissue explant. N.A. Dye1, S. Eaton1; 1MPI-CBG, Dresden, Germany 9:55 am M225 Emergent mechanisms of collective cell durotaxis. R. Sunyer1, V. Conte1, J. Escribano2, J.M. García Aznar2, J. Muñoz3, P. Roca-Cusachs1,4, X. Trepat1,4; 1Institute for Bioengineering of Catalonia, Barcelona, Spain, 2University of Zaragoza, Zaragoza, Spain, 3Polytechnic University of Catalonia, Barcelona, Spain, 4University of Barcelona, Barcelona, Spain 10:15 am M226 A novel bidirectional signaling pathway regulates collective cell migration in the Drosophila WEDNESDAY egg chamber. K. Barlan1, M. Cetera1, S. Horne-Badovinac1; 1Molecular Genetics & Cell Biology, University of Chicago, Chicago, IL 10:22 am M227 Vertebrate embryos in suspended animation - characterizing cellular and molecular mechanisms of diapause which put embryo development on hold to survive through adverse condition. C. Hu1, A. Brunet1; 1Genetics, Stanford University, Stanford, CA 10:29 am M228 Aberrant cell segregation driven by ephrin-B1 mosaicism relies on Eph/ROCK signaling and involves changes in actin polymerization. A.K. ONeill1,2, A.R. Larson1,2,3, T.K. Niethamer1,2,4, J.O. Bush1,2,5; 1Program in Craniofacial Biology, University of California, San Francisco, San Francisco, CA, 2Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA, 3School of Medicine, University of California, San Francisco, San Francisco, CA, 4Biomedical Sciences Graduate Program, University of California, San Francisco, San Francisco, CA, 5Institute for Human Genetics, University of California, San Francisco, San Francisco, CA 10:35 am M229 Planar cell polarity signaling in airway epithelial homeostasis and disease. E.K. Vladar1, J.V. Nayak2, J.D. Axelrod1; 1Pathology, Stanford University School of Medicine, Stanford, CA, 2Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA 10:42 am M230 Asymmetric partitioning of WNT and SHH signaling regulates the specification of hair follicle stem cells. T. Ouspenskaia1, I. Matos1, A.F. Mertz1, J. Levorse1, L. Polak1, E. Fuchs1; 1Laboratory of
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 135 Mammalian Cell Biology and Development, HHMI, Rockefeller University, New York, NY 10:49 am M231 Niche architecture and stiffness supports satellite cell self-renewal. R. Cheng1,2, H. Liu3, S. Davoudi1,2, C. Simmons1,3, P.M. Gilbert1,2; 1Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, 2Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, 3Department of Mechanical Engineering, University of Toronto, Toronto, ON
* Miheala Serpe is the recipient of the ASCB WICB Junior Award for Excellence in Science Research.
l Symposium 7: Beyond the Five Senses: Detection of Magnetic and Electric Fields 11:15 am-12:15 pm Ballroom 20BC
Chair: Angelika Amon, Massachusetts Institute of Technology
11:15 am S16 Cell biology of magnetic particle formation in magnetotactic bacteria. E. Cornejo1, D. Hershey1, P. Subramanian2, X. Ren3, J. Hurley3, G.J. Jensen2, A. Komeili1,3; 1Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, 2Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, 3Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 11:45 am S17 Individual and collective cell polarization and migration in electric field. Y. Sun1, K. Zhu1, Y. Sun1, B. Reid1, F. da Silva Ferreira1, Y. Li1, X. Gao1, M. Ying1, B.W. Draper1, M. Zhao1, A. Mogilner2; 1Medical School, University of California, Davis, CA, 2Courant Institute and Department of Biology, New York University, NY
136 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Exhibitors Launches The Revolutionary Label-free Tomographic Microscope — Booth #1322 Microscope Tomographic Label-free The Revolutionary able to image living cells microscope The 3D Cell is a revolutionary Explorer without need of any stain and at nanometric resolution. in 3D, instantly, New CCD Cameras and Imaging Software from QImaging — Booth #923 CCD Cameras from and Imaging Software New Ocular™ Software. Right for every R™ Series with intuitive USB3 Retiga cameras cell live imaging and documentation to advanced routine laboratory task, from imaging. — Booth #1316 Technology Z1TM Single-cell Western the Z1TM instrument which uses microfluidic Zephyrus Biosciences has developed analysis of thousands of individual protein western-based technology to allow cells in parallel. Introducing Photometrics Prime™ sCMOS CameraIntroducing — Booth # 921 engine with an embedded signal processing The first and only sCMOS camera hidden in photon shot noise. images that are restores that quantitatively Bruker’s Super-resolution Microscopy – Booth #612 – Microscopy Super-resolution Bruker’s research your is designed to advance microscope super-resolution newest Bruker’s confocal imaging. Come data and correlated see it with meaningful quantitative in action at Booth #612. New Amicon® Stirred Cell Amicon® Stirred — Booth #713 New the same Cell, Amicon® Stirred providing the new has developed EMD Millipore research your buffer thorough and exchange macrosolutes of recovery high gentle, requires. cutting-edge New Technology New Exhibitors 2414-02 Oly ASCBad_v1.indd 1 48 Woerd Avenue, Waltham, MA02453,800-446-5967 LifeScience OLYMPUS For furtherinformation,please visit:YourScienceMatters.com Your ScienceMatters Let usworktogethertocreate anidealenvironment forexploringpossibilities. clarity andvalueinmind. your laboratoryneeds.Ourlineofimagingsolutionsisbuilt withquality, We focusondevelopingandsupplyingthequalityresources tofulfill At Olympus,weunderstandthevalueofavision. Partner inDiscovery Hiroo UenoPh.D;DepartmentofStem CellPathology, KansaiMedicalUniversity;Rainbowmouse(Eachinterpapillarypitofthetongueisoccupiedbysingle-colorcellsthatoriginatefrom monoclonalstemcells.5colors:four fluorescent protein (EGFP, mCerulean,mOrange,mCherry)andnuclearstainingofHoechst 33342) Two daysaftertamoxifeninduction,theepithelial cells intheinterpapillarypitexpress randomcolors,indicatingthatmultiple clones proliferated independently. However, after84days,each interpapillarypitwasoccupiedbysingle-colorcells,indicatingthattheyare derivedfrom monoclonalstemcells.(Nature CellBiology15,511–518,2013) ™ 10/21/15 2:04 PM Exhibit Hours Sunday, Dec. 13 9:30 am–4:00 pm Monday, Dec. 14 9:30 am–4:00 pm Tuesday, Dec. 15 9:30 am–4:00 pm
Remember, all activities take place in the ASCB Learning Center from 12:00 pm to 3:00 pm.
The ASCB gratefully acknowledges the support of all exhibiting companies and supporters.
The ASCB Exhibitor Advisory Committee will meet during the 2015 ASCB Meeting. Exhibitor Advisory Committee members can be identified at the meeting by the “Ocean/Blue/Gold” colored ribbon on their meeting badge. Questions or concerns about your 2015 experience? Suggestions on how we can serve you better? Locate one of our advisory members below or contact Louise Campbell-Blair in the Exhibit Lounge and have a conversation.
Meet Your Exhibitor Advisory Committee Committee Members:
Jim Zacka Elaine Fitzpatrick Chuck Locke (Chair) Worthington Biochemical Trade Show Project Drummond Scientific Corporation Manager Booth 425 Booth 628 EMD Millipore Corp Booth 713
Sande Giaccone Cyndy Nawrocki Louis Shulman Jacqueline M. Seltzer GEN Group Publisher Marketing Communications Olympus America W.H. Freeman & Company Booth 1001 Director Booth 813 Booth 1214 Photometrics | QImaging Booth 921/923
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 137 EXHIBITORS
138 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting EXHIBITORS
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 139 EXHIBITORS
Descriptions were provided by exhibiting Abcam 934 Adipogen Life Sciences 621 companies. One Kendall Square 11588 Sorrento Valley Road Suite B2304 Building 200 Suite 16 3D Biotek, LLC 301 Cambridge, MA 02139 San Diego, CA 92121 1 ILENE Court Phone: 888-77-ABCAM Phone: (858) 457-8383 Building 8 Unit 12 www.abcam.com www.adipogen.com Hillsborough, NJ 08844 Phone: (732) 729-6270 Abcam plc is a global leader in the supply of life Manufacturer of life science reagents in the www.3dbiotek.com science research tools, with an unrivaled range of areas of metabolism, obesity, cancer biology, products and expert technical support, enabling cell signaling, inflammation, stem cell biology, Headquartered in New Jersey, USA, 3D Biotek scientists to analyze living cells at the molecular oxidative stress, and autophagy. A major focus is engaged in developing 3-dimensional cell level and improving the understanding of health is on high quality proteins, advanced ELISA kits, culture scaffolds by using its precision 3D micro- and disease. To find out more, please visit www. recombinant antibodies, and small molecules. We fabrication and advanced bio-manufacturing abcam.com. collaborate with Chemodex (fluorescent probes, technology. The revolutionary 3-dimensional stains, labels, dyes). www.adipogen.com. porous cell culture scaffolds are powerful tools for AbD Serotec, 536 stem cell, tissue engineering, drug discovery, and A Bio-Rad Labortories Company Advanced BioMatrix, Inc. 626 biopharmaceutical research applications. 4350 Lassiter At North Hills Avenue P.O. Box 502403 Suite 250 San Diego, CA 92150 3H Biomedical AB 335 Raleigh, NC 27609 Phone: (800) 833-8220 Dag Hammarskjolds vag 34A www.abdserotec.com www.advancedbiomatrix.com Uppsala Science Park Uppsala, 751 83 Sweden AbD Serotec, a Bio-Rad company, is a trusted Advanced BioMatrix is a leader in life science www.3hbiomedical.com antibody producer and supplier to healthcare 3D applications for tissue culture and cell industry experts and researchers. Whether you proliferation. Our products are recognized as the 3H Biomedical is an innovative life-science need fast access to a huge range of ready- standard for purity, functionality, consistency. company that develops, manufactures, and made antibodies or custom antibodies tailored to We offer premiere collagen products, hydrogels, distributes human and animal cell-based products your precise research requirements, we have a and attachment factors. Our products provide for life science research and drug development. reputation for product excellence and unrivaled an in vivo-like 3D matrix environment for cell We are a leading global supplier of more than 180 customer support. attachment and proliferation. different types of primary human cells, cell culture media, RNA, genomic DNA and proteins. Accurate Chemical 1006 Allele Biotechnology 903 & Scientific Corporation & Pharmaceuticals Inc 3i Intelligent Imaging Innnovations 741 300 Shames Drive 6404 Nancy Ridge Dr. 3509 Ringsby Court Westbury, NY 11590 San Diego, CA 92121 Denver, CO 80216 Phone: (800) 645-6264 Phone: (858) 587-6645 Phone: (303) 607-9429 www.accuratechemical.com www.allelebiotech.com www.intelligent-imaging.com Accurate Chemical & Scientific Corporation has Allele Biotech is a San Diego based biotech 3i designs and manufactures technologies for been supplying research facilities worldwide company with a mission to increase accessibility live-cell and intravital fluorescence microscopy with a full line of Laboratory Research Products to innovative molecular biology research tools. including digital holography, spinning disk that include Antibodies, Antigens and Kits, Cell We have a wide array of research and commercial confocal, multi-photon, and lightsheet. SlideBook Separation Media & Uni-Sep Tubes, Chick reagents and services for drug discovery including software manages everything from instrument Embryo Extracts, Complements and Mouse cellular reprogramming, assay development, control to image capture, processing, and data Reagents, Complex Carbohydrates, HeLa Cells, camelid derived nano-antibodies, and much more. analysis. Electrophoresis Units, Extracellular Matrix Assays, and Water Baths. alvéole 1104 AAT Bioquest Inc. 507 68 boulevard de Port Royal 520 Mercury Drive ACEA Biosciences, Inc. 1412 Paris, 75005 France Sunnyvale, CA 94085 6779 Mesa Ridge Road Phone: (408) 733-1055 Suite 100 alvéole offers advanced solutions that combine www.aatbio.com San Diego, CA 92121 the latest innovations in cell imaging and Phone: (858) 724-0928 microfluidics for controlling various parameters of AAT Bioquest offers bioanalytical reagents. We www.aceabio.com living cells microenvironnement. We will present specialize in fluorescence and luminescence- PRIMO, our new compact, versatile and easy-to- based detection technologies. Our products ACEA Biosciences, Inc. provides high use UV-photopatterning device for multiproteins include the outstanding Fluo-8®, Cal-520™, Cal- performance, affordable flow cytometers, printing on various cell culture surfaces (coated 590™ and Cal-630™ calcium imaging indicators. and real-time cell analysis instrumentation glass, plastic, PDMS). We also offer other fluorescence imaging and flow for efficient monitoring of cell-based assays. cytometry products, such as JC-10™, iFluor™ ACEA’s xCELLigence® and NovoCyte™ systems American Physiological Society 1137 labeling dyes, cell viability, tracing and tracking are used in pre-clinical drug discovery and 9650 Rockville Pike probes, and kits. development, toxicity, safety pharmacology, and Bethesda, MD 20814 basic academic research. www.the-aps.org Abbiotec 948 7985 Dunbrook Rd., Ste A The American Physiological Society (www. San Diego, CA 92126 the-aps.org) is a nonprofit devoted to fostering www.abbiotec.com education, scientific research, and dissemination of information in the physiological sciences. As researchers are focusing their efforts on Featured is one of APS’ cutting-edge journals, determining the biological functions, interactions, AJP-Cell Physiology (www.ajpcell.org). Stop and regulation of the human proteome, Abbiotec by the booth to meet the editor, pick up journal is dedicated to developing cell biology-based information, and get your free meeting essentials! research tools that assist scientific discoveries. Besides custom peptide and antibody services, Abbiotec offers over 12,000 antibodies, proteins, peptides, lysates, and kits.
140 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting EXHIBITORS
American Society for Cell Biology 721 ASI/Applied Scientific Instrumentation 601 Baker 927 8120 Woodmont Ave 29391 West Enid Road P.O. Drawer E Suite 750 Eugene, OR 97402 Sanford, ME 04073 Bethesda, MD 20814 Phone: (541) 461-8181 Phone: (207) 324-8773 Phone: (301) 347-9300 www.asiimaging.com www.bakerco.com www.ascb.org ASI manufactures top-of-the-line hardware for For more than 60 years, Baker has helped The ASCB Booth is your one-stop shop for all sub-micron micropositioning and microscopy advance science, discovery, and clinical care things ASCB. Browse our selection of ever- applications, including XYZ stages for ultra- by pioneering innovative solutions for air popular t-shirts and merchandise, talk to ASCB precise sub-micron positioning, stages with piezos containment, contamination control, and controlled staff about all the benefits and programs the for high resolution/speed positioning, autofocus environments. Baker biological safety cabinets, organization offers, and find out what we can do systems, optics & illumination, light sheet clean benches, fume hoods, and pharmacy for you! microscopes/iSPIM & diSPIM systems and other isolators provide unparalleled user and product custom microscope/opto-mechanical systems. We protection for a variety of applications. AnaSpec, Inc. 328 utilize long life components for demanding OEM 34801 Campus Drive applications. B-Bridge International, Inc. 502 Fremont, CA 94555 3350 Scott Blvd #29 Phone: (510) 791-9560 Atlas Antibodies 841 Santa Clara, CA 95054 www.anaspec.com AlbaNova University Center Phone: (408) 252-6200 Stockholm, 10691 Sweden www.b-bridge.com AnaSpec, EGT Group is a leading provider of atlasantibodies.com integrated proteomic and genomic solutions™ for B-Bridge offers innovative cell biology tools, worldwide life science research. We offer products Atlas Antibodies is a Swedish manufacturer and including unique mechanical cell strain and expertise in peptides, fluorescent dyes, supplier of highly characterized antibodies and instruments, hydrogel for 3D cell culture, and SensoLyte® assay kits, antibodies – primary, isotope-labeled mass spectrometry standards non-magnetic cell separation system that makes including zebrafish, and secondary (chemical or targeting all human proteins. Originally developed isolating target cells a breeze. Visit us to learn dye-labeled) antibodies, unusual amino acids, and and validated within the Human Protein Atlas about our extensive selection of cells and cell Takyon™ qPCR MasterMixes. (HPA) project, our 18,000 antibodies come with lines, sample prep kits, cell-based assays, a vast quantity of characterization data freely ELISAs, and more. Andor Technology 744 available on the HPA portal. 7 Millenium Way BD Biosciences 303 Belfast, BT12 7AL UK Aviva Systems Biology Corporation 849 2350 Qume Drive www.andor.com 5754 Pacific Center Blvd San Jose, CA 95131 Suite 201 Phone: (858) 812-8884 Andor Technology is a global leader in San Diego, CA 92121 www.bd.com the pioneering and manufacturing of high Phone: (858) 875-4953 performance scientific imaging cameras, www.avivasysbio.com BD Biosciences is a world leader in bringing spectroscopy solutions and microscopy systems innovative diagnostic and research tools to for research and OEM markets. Andor Technology Aviva Systems Biology Incorporated specializes life scientists, clinical researchers, laboratory is part of Oxford Instruments plc, a leading in providing polyclonal and monoclonal antibodies professionals, and clinicians who are involved in provider of high technology tools and systems for for research needs. Unlike other companies, basic research, drug discovery and development, industry and research. we design, manufactureand validate our own biopharmaceutical production, and disease antibodies. Our head office is in San Diego, management. Applied BioPhysics Inc. 600 California, and provides scientific support 185 Jordan Road assisting researchers with a variety of proteomic Beckman Coulter Genomics 505 Troy, NY 12180 objectives. 36 Cherry Hill Drive Phone: (518) 880-6860 Danvers, MA 01923 www.biophysics.com Axis-Shield PoC 907 www.beckmangenomics.com PO Box 6863 Rodelokka ECIS (Electric Cell-Based Impedance Sensing) Oslo, N-0504 Norway Beckman Coulter Genomics, headquartered in is a real-time, impedance-based method to study Phone: 011-47-240-56000 Danvers, Massachusetts, sets the standard for the behavior of cells grown in tissue culture. www.axis-shield.com providing expert Next Generation and Sanger Assays include TEER, migration, proliferation, sequencing services with bioinformatics solutions. signal transduction, cell differentiation, phenotypic Axis-Shield will display a range of Density Life science and healthcare businesses as well as assays, cell toxicity, and stem cell biology as well Gradient Media for the isolation of cells, academic and government institutions worldwide as cell behavior under dynamic flow conditions. subcellular membranes, organelles, viruses and rely on Beckman Coulter Genomics to deliver the Measurements are continuous and label free. macromolecules using centrifugation techniques. highest quality data, and unparalleled customer service. ASCB Journals 1226 Azure Biosystems 625 Molecular Biology of the Cell and CBE - Life 6773 Sierra Ct Beckman Coulter Life Sciences 1010 Sciences Education Suite B 250 South Kraemer Blvd 8120 Woodmont Avenue Dublin, CA 94568 Brea, CA 92821 Suite 750 Phone: (510) 676-1499 Phone: (714) 342-9074 Bethesda, MD 20814 www.azurebiosystems.com www.beckman.com Phone: 301-347-9300 www.ascb.org Azure Biosystems is focused on the development Beckman Coulter Life Sciences markets and commercialization of state-of-the-art instrument systems, reagents, and services MBoC (Molecular Biology of the Cell) and CBE- technologies to support research in the life that enable new discoveries in biology-based Life Sciences Education (LSE) are the prestigious science community. Founded in Dublin, California, research and development. Our products are research publications of the ASCB. MBOC is the Azure Biosystems is developing a series of used in the forefront of genomics and proteomics. journal for the best in cell biology and biomedical instruments for image capture and analysis. We offer an extensive range of products research. MBoC publishes important research in including: centrifugation, flow cytometry, particle all areas of cell biology and life sciences, from characterization, cell counting, and laboratory biophysics to genetics to neuroscience. LSE is automation. the open-access journal for excellence in biology education research and evidence-based teaching. It is for educators at all levels and across all life science disciplines.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 141 EXHIBITORS
Bellco Glass, Inc. 319 Biosearch Technologies, Inc. 643 Biotium, Inc. 720 340 Edrudo Road 2199 South McDowell Blvd 3159 Corporate Place Vineland, NJ 08360 Petaluma, CA 94954-6904 Hayward, CA 94545 www.bellcoglass.com Phone: (415) 883-8400 Phone: (510) 265-1027 www.biosearchtech.com www.biotium.com Bellco Glass, Inc. is a leader and pioneer in the design and manufacturing of biological glassware Biosearch Technologies offers sophisticated Biotium is an innovative life science technology and equipment. Bellco will serve the needs of all oligonucleotide-based tools for real-time PCR and company specialized in developing and biological research and discovery throughout the Stellaris® RNA FISH. Stellaris RNA FISH makes manufacturing new fluorescence-based detection world. We are a supplier of premium products that use of multiple, singly-labeled oligonucleotides reagents. Our products are widely used in many surpass expectations of our customers conforming to enable localization and quantification of RNA important biological research fields, medical to all established requirements. at the single cell and molecule level. Scientists diagnostics, and pharmaceutical science. can visualize RNA in cells and tissue without BINDER, Inc. 605 purification, reverse transcription, or amplification. Bitplane, Inc. 746 545-3 Johnson Avenue 7 Millenium Way Bohemia, NY 11716 Bioss Inc. 520 Belfast, BT12 7AL UK Phone: (631) 224-4354 500 West Cummings Park Phone: 860-290-9211 www.binder-world.com Suite 6500 www.bitplane.com Woburn, MA 01801 BINDER is the world’s largest specialty www.biossusa.com Bitplane is the world’s leading interactive manufacturer of CO2 and microbiological microscopy image analysis software company. incubators, growth chambers, ultra-low Bioss Inc is a leading antibody developer whose Through constant innovation on 3D/4D image temperature freezers, ovens and constant mission is to develop and manufacture top quality visualization and analysis, Bitplane actively climate chambers for science and industrial antibodies that accelerate biological research and shapes the way scientists process multi- laboratories. Our products are found in biotech, discovery. dimensional microscopic images. Bitplane is part pharmaceutical, chemical/food industry and of Oxford Instruments, a leading provider of high research labs for environmental analysis, Bio-Techne 1127 technology tools and systems for industry and industrial testing, and quality assurance. 614 McKinley Place NE research. Minneapolis, MN 55413 BioChain Institute, Inc 407 Phone: (612) 379-2956 BMG Labtech 726 39600 Eureka Dr. www.bio-techne.com 13000 Weston Parkway Newark, CA 94560 Suite 109 www.biochain.com Bio-Techne combines the best-in-class products Cary, NC 27513 and services from R&D Systems, Tocris Phone: (877) 264-5227 For 20 years, BioChain has been focusing on Bioscience, and Boston Biochem to allow www.bmglabtech.com high quality bio-sample products, tools for sample us to become better strategic partners with preparation and analysis, and custom services researchers. We manufacture over 95% of our BMG Labtech has been committed to producing used to accelerate the development of cancer bioactive proteins, application-qualified antibodies, microplate readers for more than twenty years. By diagnostics, therapeutics, and research. BioChain Quantikine ELISAs, Luminex Assays, small focusing on the needs of the scientific community, also provides pcr reagents, pre-screened NGS molecules or Ubiquitin related products. We are the company’s innovative microplate readers have cancer samples, and automated nucleic acid Bio-Techne. earned the company the reputation of being a extraction system. technology leader in the field. BioTechniques 515 BioLegend 504 52 Vanderbilt Avenue Boeckeler Instruments Inc. 749 9727 Pacific Heights Blvd. New York, NY 10017 4650 S. Butterfield Drive San Diego, CA 92121 Phone: (212) 520-2714 Tucson, AZ 85714 Phone: (858) 455-9588 www.informausa.com www.rmcproducts.com www.biolegend.com BioTechniques, the international journal of life RMC-Boeckeler manufactures ultramicrotomes, BioLegend develops and manufactures world- science methods, provides open access to cryo-ultramicrotomes and related sample class, cutting-edge antibodies and reagents first-quality, peer-reviewed papers on laboratory preparation instruments for most microscopical at an outstanding value. The broad product techniques and protocols. Now in its 58th volume, imaging modalities. For high resolution, 3D portfolio includes flow cytometry, cell biology, and BioTechniques has over 80,000 print subscribers SEM cellular ultra-structural reconstructions, functional reagents for research in immunology, worldwide. The journal augments its peer- RMC-Boeckeler offers the Harvard-developed neuroscience, cancer, and stem cells. Custom reviewed content with feature articles and topic- automated serial section tape collecting services include assay development, sample specific supplements. Visit www.biotechniques. ATUMtome that will be on display at the ASCB testing, and conjugation. com for more details. 2015 meeting in San Diego.
Bio-Rad Laboratories 535 BioTek Instruments, Inc. 702 Boster Immunoleader 649 2000 Alfred Nobel Drive Highland Park 3942 Valley Ave Ste B Hercules, CA 94547 Box 998 Pleasanton, CA 94566 Phone: (510) 741-1000 Winooski, VT 05404 Phone: (510) 445-1120 www.bio-rad.com Phone: (802) 861-8637 www.bosterbio.com www.biotek.com Providing instrumentation and reagents to Boster Immunoleader has developed and support life science research. Depend on BioTek® is a global leader in the design, validated a wide range of high quality Bio-Rad for tools, technologies and expertise manufacture, and sale of microplate immunological reagents; more than 5,000 to enable genomic and proteomic analysis. instrumentation and software. BioTek research publications have cited Boster products. Products for droplet digital PCR, conventional instrumentation is used to aid in the advancement Our reagents are optimized for WB, IHC, and real-time PCR, SPR, transfection, of life science research, facilitate the drug ELISA, and a variety of other applications in RNAi, biomarker expression profiling, xMAP discovery process, and enable cost-effective cancer, developmental biology, neuroscience, technology, cancer biomarkers, expression quantification of disease relevant molecules in the immunology, and more. proteomics, electrophoresis, blotting-systems, clinic. chromatography, imaging, cell-biology antibodies.
142 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting EXHIBITORS
Bruker Nano Surfaces 612 Cell Applications Inc. 326 Celprogen Inc. 402 3400 E. Britannia Dr. 5820 Oberlin Drive 3914 Del Amo Blvd, Ste 901 Suite 150 Suite 101 Torrance, CA 90503 Tucson, AZ 85706 San Diego, CA 92121 Phone: (310) 542-8822 Phone: (805) 967-2700 www.cellapplications.com www.celprogen.com www.bruker-nano.com Cell Applications Inc. is a global authority Celprogen is a biotechnology company developing Bruker provides a wide range of fluorescence and leading provider of primary cells for stem cell research and therapeutics products for microscopy systems for advanced life science pharmaceutical drug discovery, biotechnology, regenerative medicine in cardiology, oncology, applications. The new Opterra II swept-field and life science R&D. Our full line of cell biology diabetes and neurology. All of Celprogen’s microscope is the next generation confocal for products includes optimized growth media, biological products, including stem cells, cancer live-cell applications. The Vutara super-resolution reagents, antibodies, cytokines, kits, RNA, and stem cells, iPCs and media including ECMs, are system features the fastest single-molecule custom services. CAI supplied cells for 2012 manufactured and produced in the United States localization (SML) capabilities available for deep Nobel Prize winner Yamanaka. of America for Life Science. live-cell investigation. Cell Biology in France (SBCF) 1529 Chemglass Life Sciences 1021 Bulldog Bio Inc. 312 16 Boulevard St. Germain 3800 North Mill Road One New Hampshire Avenue Paris, 75005 France Vineland, NJ 08360 Suite 125 Phone: 011-33-4939-57767 Phone: (800) 843-1794 Portsmouth, MA 03801 www.cglifesciences.com Phone: (603) 570-4248 SBCF (Société Française de Biologie Cellulaire) is www.bulldog-bio.com a community of >1250 biologists studying the cell, Chemglass Life Sciences is a woman owned the fundamental unit of life. We are dedicated to business. We offer life science industry solutions Bulldog Bio provides quality products for advancing scientific discovery, advocating sound including; spinners and magnetic stirrers, cell life science research. On display will be the research policies, improving education, promoting culture bags, bioreactors, microbial culture ELOPO21 from Nepa Gene – a remarkably professional development. We are fostering flasks, bench equipment, rollers, rockers, PCR versatile electroporator able to deliver DNA/RNA relationships with similar communities in Europe accessories, spectrophotometers, incubators, to immortalized and primary cells, in addition to and around the world. plastic ware, reusable glassware, ovens, walled cells. Also, we’ll be demonstrating how refrigerators, freezers and racks, disposable the PicoPipet can pipet individual cells, intact and Cell Press 1246 flasks, shake flasks, and vials. alive. 600 Technology Square Cambridge, MA 02139 Chroma Technology 627 Caliber Imaging & Diagnostics Inc 843 Phone: (617) 661-7057 10 Imtec Lane 100 Burtt Road, Suite 203 www.elsevier.com Bellows Falls, VT 05101 Andover, MA 01810 Phone: (800) 824-7662 Phone: (585) 239-9800 Cell Press is the home for cell biologists, offering www.chroma.com www.caliberid.com high-quality, cutting-edge cell biology research and resources to propel your work forward. We Chroma Technology designs and manufactures A leader in imaging innovation with over 20 years publish 14 primary research journals, 14 Trends optical interference filters using advanced of confocal microscopy experience, Caliber I.D. reviews journals, and four primary research sputtering technologies. Our high performance introduces its latest breakthrough in confocal journals on behalf of learned societies. Make filters are intended for imaging applications microscopy, the RS-G4. With applications in yourself at home at Cell Press booth #1246. ranging from widefield and confocal fluorescence neuroscience, developmental biology, pathology microscopy, TIRF and super-resolution techniques and translational research, this high-performing Cell Signaling Technology 706 to flow cytometry, high content screening, system was developed exclusively for the 3 Trask Lane multi-photon and Raman spectroscopy. Chroma research scientist. Visit booth 843 or www. Danvers, MA 01923 also provides comprehensive technical and caliberid.com. Phone: (978) 867-2300 applications support. www.cellsignal.com Carl Zeiss Microscopy, LLC 735 ChromoTek GmbH 603 One Zeiss Drive CST is a private, family-owned company founded Am Klopferspitz 19 Thornwood, NY 10594 by scientists and dedicated to providing high Martinsried, 82152 Germany Phone: (800) 233-2343 quality research tools to the biomedical research Phone: 011-49-89-7879-7310 www.zeiss.com/microscopy community. Our employees operate worldwide www.chromotek.com from our U.S. headquarters in Massachusetts, and As the world’s only manufacturer of light, our offices in the Netherlands, China, and Japan. Immunoprecipitaton of GFP- and RFP-fusion X-ray and electron/ion microscopes, ZEISS proteins: Nano-Traps® Immunoprecipitation of offers tailor-made microscope systems for 3D Cellecta, Inc. 634 selected targets: Nano-Traps® Super Resolution imaging in biomedical research, life sciences, 320 Logue Avenue Microscopy: Nano-Booster Protein-protein and healthcare. A well-trained sales force, an Mountain View, CA 94043 interactions: Live visualization with our F2H®- extensive support infrastructure, and a responsive Phone: (650) 938-3910 Assay Real-time observation of endogenous service team enable customers to use their ZEISS www.cellecta.com cellular structures: Chromobodies® Working high microscopes to their full potential. quality antibodies ChromoTek GmbH pioneers a Cellecta provides HT loss-of-function genetic new class of immunologic research tools derived Cedarlane 512 screening services for the discovery and from single domain camel antibodies! 1210 Turrentine Street functional characterization of novel therapeutic Burlington, NC 27215 targets. We offer pooled lentiviral shRNA, Phone: (800) 721-1644 CRISPR, and cell tracking barcode libraries, www.cedarlanelabs.com pooled library screening and analysis by NGS, individual lentiviral expression constructs, and Providing today’s researchers with the newest, stable reporter, overexpression, knockdown, and high quality products, Cedarlane is a vital knockout constructs and cell lines. resource to the Life Science industry. Cedarlane’s customers take advantage of access to millions of products from top global suppliers. Open six days a week, we strive to save you money through consolidation and timely, affordable delivery.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 143 EXHIBITORS
Cincinnati Children’s Research Foundation 1040 Cold Spring Harbor Laboratory Press 1234 DDNews 905 3333 Burnet Avenue One Bungtown Road 19035 Old Detroit Road Cincinnati, OH 45229 Cold Spring Harbor, NY 11724 Suite 203 Phone: (513) 636-7459 Phone: (516) 422-4005 Rocky River, OH 44116 www.cincinnatichildrens.org www.cshlpress.org Phone: (440) 331-6600 www.ddn-news.com Cincinnati Children’s Hospital Medical Center’s Cold Spring Harbor Laboratory continues to shape vision is to be THE LEADER in improving contemporary biomedical research and education DDNews is an international news organization child health. Scientists at Cincinnati Children’s with programs in cancer, neuroscience, plant reporting trends and developments impacting the Research Foundation conduct cutting edge basic, biology, and quantitative biology. Its Meetings business of Pharma, Biopharma and Life Science translational, and clinical research. We offer & Courses annually hosts over 8,000 scientists researchers from the bench to the boardroom. Postdoctoral opportunities, graduate programs from around the world; its Press publishes books, From a single business news publication, in Immunobiology, Molecular Developmental journals, and electronic media for scientists, DDNews has grown to twelve print and online Biology, and Genetic Counseling and students, and the general public. news vehicles. Free subscriptions at http://online. undergraduate summer research opportunities. icnfull.com/orp/. CoolLED 1026 Cloud-Clone Corp 1002 Westmarch Business Centre De Novo Software 624 1304 Langham Creek Dr. River Way 400 North Brand Blvd Suite 226 Andover Hants, SP10 1NS UK Suite 850 Houston, TX 77084 Phone: 011-44-1264-320989 Glendale, CA 91203 Phone: (832) 606-1724 www.coolled.com Phone: (213) 814-1240 www.cloud-clone.us www.denovosoftware.com CoolLED designs and manufactures a Cloud-Clone Corp. is a biotechnology company. comprehensive range of cutting edge LED FCS Express 5 is the premiere Flow and Image Our products cover from genes to proteins, illumination systems for bioscience and clinical cytometry data analysis software. Powerful include cDNA libraries, Primers, Proteins and microscopy including: pE-100 series, single analysis tools, flexible visualization capabilities DNA Marker, Natural and Recombinant proteins, wavelength illumination systems. pE-300white and sophisticated presentation features make it Monoclonal and Polyclonal antibodies, Target series, broadspectrum illumination. pE-4000 – the the obvious choice for thousands of researchers gene clone Kits, ELISA and CLIA Kits. We also universal LED illumination system for research needing quick and accurate results. Get up to offer custom-built services to meet our customers’ fluorescence. speed fast with excellent customer service and specific needs. online educational support. Cosmo Bio USA 1024 Cluster of Excellence BIOSS 1328 2792 Loker Avenue West, Suite 101 DeNovix Inc. 405 - Centre for Biological Signalling Studies Carlsbad, CA 92010 3411 Silverside Road University of Freiburg Phone: (760) 431-4600 Hanby Building Schänzlestr. 18 www.cosmobiousa.com Wilmington, DE 19810 Freiburg, 79104 Germany Phone: (302) 442-6911 www.bioss.uni-freiburg.de Great reagents drive great research. If we have www.denovix.com what you need, you’ll want what we have. Come The main focus of research at BIOSS is to cruise our collections. Fine biological research DeNovix Inc. develops, manufactures and sells initiate and promote a dialectic process between reagents, assays, and biotools sourced from +80 laboratory equipment for life science applications. scientists using analytical (dissecting) and Japanese manufacturers. Also representing Axis- Our stand-alone instruments include 1µL UV-Vis synthetic (rebuilding) approaches in signalling Shield (Norway, density gradient media), Cusabio Spectrophotometers, Fluorometers and the research. Signal pathway engineering projects (China, assays), and Ubiquigent (UK, ubiquitin- DS-11 FX+ with 1µL UV-Vis, cuvette UV-Vis and are conducted by an interdisciplinary team of proteasome reagents) in North America. Fluorescence combined. Instruments include an researchers and are supported by a new resource Android HD touchscreen interface and built-in Wi- centre called the Toolbox. Cosmo Biotechnologies LTD 421 Fi, Ethernet and USB. 1633,Bayshore Hwy, STE 336 Cluster of Excellence CellNetworks - 1229 Burlingame, CA 94010-1515 Diagenode 403 Heidelberg University www.cosmobrand.com 597 Tami Way Bioquant BQ002, Room 743 Mountain View, CA 94041 Im Neuenheimer Feld 267 Cosmo is a professional manufacturer and CMO www.diagenode.com Heidelberg, D-69120 Germany of plasticware for life science research use and Phone: +49 (0) 6221-54 5120 medical devices. Diagenode, the leading provider of complete www.cellnetworks.uni-hd.de solutions for epigenetics research, offers Cytoskeleton, Inc. 1134 innovative shearing and automation instruments, CellNetworks strives to craft a highly interactive 1830 South Acoma Street reagent kits, and high quality antibodies to community of scientists from life sciences, physics Denver, CO 80223 streamline DNA methylation, ChIP, and ChIP-seq and engineering, mathematical and computational Phone: (303) 322-2254 workflows. Our latest innovations include a full sciences, to empower them to tackle fundamental www.cytoskeleton.com automation system, ChIP-seq kits for only 10,000 questions in cellular network architecture, cells, and the industry’s most validated antibodies. dynamics, regulation, and interaction using exact Cytoskeleton, Inc. provides highly active quantitative methods. preparations of pure actins, tubulins, motor Drummond Scientific 434 proteins, and small G-proteins along with 500 Parkway functional assay kits for these proteins to study Broomall, PA 19008 cytoskeletal structure and function. We also Phone: (800) 523-7480 offer custom protein production and compound [email protected] screening services. All products are stringently quality-controlled and available for bulk purchase. Drummond will be introducing its newest microinjector, the Nanoject 3. Stop by our booth to see the new features and capabilities offered in the new model. See the new electronics and the increased injection capabilities. Visit our booth to see the unit in action.
144 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting EXHIBITORS
DRVision Technologies LLC 501 Electron Microscopy Sciences 1514 Essen BioScience 440 15921 NE 8th Street, Suite 200 1560 Industry Road 300 West Morgan Road Bellevue, WA 98008 Hatfield, PA 19440 Ann Arbor, MI 48108 Phone: (425) 653-5589 Phone: (215) 412-8400 Phone: (734) 769-1600 www.svcell.com www.emsdiasum.com www.essenbioscience.com
DRVision software, SVCell, changes the way Electron microscopy sciences will have on IncuCyte™ live-cell imaging systems, designed image recognition is done - enabling users to display its complete line of accessories, by Essen BioScience, is the first instrument to develop and execute analyses of unsurpassed chemicals, supplies and equipment for all fields provide automated real-time image capture and quality, without image processing expertise. of microscopy, biological research and general data analysis from hours to weeks, directly inside Simple to learn and operate, SVCell provides laboratory requirements, as well as its full line of your incubator. The IncuCyte™ systems also offer everything you need to detect, track, measure and tools, tweezers and dissecting equipment. a variety of software modules as well as in-vitro classify objects in microscopy images. assays, reagents, consumables and customized Embi Tec 1420 services. eBioscience, An Affymetrix Business 1413 7738 Arjons Drive 10255 Science Center Drive San Diego, CA 92126 Essential Pharmaceuticals LLC 748 San Diego, CA 92121 www.embitec.com 100 Princeton South Corporate Center Phone: (888) 899-1371 Ewing, NJ 08628 www.echo-labs.com Embi Tec manufactures and distributes equipment www.essentialpharma.com for the modern-day lab: The ultra-compact Discover diversity by linking key molecules to RunOne Electrophoresis System with built-in Essential Pharmaceuticals offers quality cell defined cellular functions through single-cell power supply, MultiCaster Systems for casting culture media products for the next generation isolations, OMICs analysis and expression agarose gels, ViewOne LabLite for backlighting of research. Now cell culture can be conducted validation. Solutions and platforms consist of samples, LightOne Illuminator pipette-aids for with all the benefits and none of the variables or protein and gene expression detection systems error-free 96/384-well setup and the PrepOne negative consequences of serum use. Cell-Ess® including miRNA analysis. Learn new methods Sapphire for direct visualization of DNA gels serum replacement is chemically defined and fully of detecting multiple RNA and protein in FFPE, without UV. synthetic. Be in control. Be certain. frozen tissue sections, or circulating tumor cells. EMBO Press 1240 Etaluma, Inc. 935 Echo Laboratories 425 Meyerhofstrasse 1 3129 Tiger Run Court 1202 Knoxville St Heidelberg, 69117 Germany Suite 112 San Diego, CA 92110 http://embopress.org Carlsbad, CA 92010 www.echo-labs.com www.etaluma.com An editorially independent publishing platform Echo Laboratories designs, develops, and for the development of the EMBO scientific Etaluma develops digital fluorescent microscopes sells microscopes. Our cornerstone product, publications: The EMBO Journal, EMBO Reports, called Lumascopes that constitute a dramatic the Revolve, combines the functionality of Molecular Systems Biology & EMBO Molecular new concept combining high resolution, more both Upright and Inverted microscopes. The Medicine. The journals publish important efficient design, and greater versatility, including Revolve also leverages tablet and cloud-based advances in the life sciences from around the time-lapse and videos. Options now include full technologies to capture and manage image data globe through a transparent and fair editorial peer- automation, and the compact design provides – setting a new precedent in microscope usability review process. live cell imaging in incubators, hoods, and other and design. challenging locations. EMD Millipore Corporation 713 EditBIOMED 1217 290 Concord Road European Research Council 1527 2141 Bluff Rd. Billerica, MA 01821 Cov2 241028 Hoover, AL 35226 Phone: (978) 715-1296 Brussels, B-1049 Belgium www.editbiomed.com www.emdmillipore.com Phone: 011-44-1189-464449 http://erc.europa.eu/ We specialize in editing and proofreading EMD Millipore is the Life Science division of scientific journal articles, grants, and manuscripts. Merck KGaA of Germany, supporting research, The European Research Council funds top Our editors are highly trained in a wide variety development and production of biotech and researchers, of any nationality, to pursue their of biomedical disciplines. With a thorough pharmaceutical drug therapies. With trusted pioneering ideas at the frontiers of knowledge. understanding of science and a sharp eye for platforms and technologies for model system It encourages highest quality research through English, we have the expertise to help you clearly development, protein preparation and detection, competitive funding supporting investigator- communicate your ideas and results. we support our customers in multianalyte network initiated frontier research across all fields of elucidation, insightful cellular analysis and research, on the basis of scientific excellence. Elabscience Biotechnology Co., Ltd 1027 functional genomics. Building 4, Room 403 FASEB 1521 Guandong Science and Technology Industry Park Enzo Life Sciences 606 9650 Rockville Pike Wuhan, Hubei Province 430073 China 10 Executive Blvd Bethesda, MD 20814-3998 www.elabscience.com Farmingdale, NY 11735 Phone: (734) 634-7288 Phone: (631) 694-7070 www.faseb.org Elabscience specializes in immunodiagnostic www.enzolifesciences.com technology for the Life Science community. We As the nation’s largest coalition of biomedical have complete R&D and manufacture platform With over 30 years’ experience, Enzo Life researchers, FASEB represents 27 scientific and have good quality control for every kit by Sciences Inc is a proven leader in labeling and societies, over 125,000 researchers, and every lot.Our major products are ELISA kits, CLIA detection technologies across research and is recognized as the policy voice of life kits, Antibodies, Proteins,Labeling kits and related diagnostic markets. Our Cellestial® assays science researchers. Join us at our Science reagents. and probes have been optimized for the most Research Conferences – a series of intimate, demanding imaging applications including interdisciplinary conferences that explore new fluorescence microscopy, flow cytometry, and approaches in research undergoing rapid scientific high content screening. advances.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 145 EXHIBITORS
Faxitron 1434 Flexcell International Corporation 622 Gene Tools, LLC 1428 3440 East Britannia Drive 2730 Tucker Street, Suite 200 1001 Summerton Way Suite 150 Burlington, NC 27215 Philomath, OR 97370 Tucson, AZ 85706 Phone: (919) 732-1591 Phone: (541) 929-7840 Phone: (520) 399-8180 www.flexcellint.com www.gene-tools.com www.faxitron.com Flexcell International specializes in products to Gene Tools manufactures Morpholino oligos Faxitron is the world’s largest dedicated cabinet apply mechanical load, (tension, compression, for blocking translation, modifying splicing or X-ray company. As a specialist in Imaging and and fluid shear), to cells in monolayer and inhibiting miRNA activity. Morpholinos are used Irradiation, Faxitron offers compact bench-top 3D culture… specifically, equipment and in cell cultures, embryos or, as Vivo-Morpholinos, cellular irradiation systems, as well as high consumables for creating environments to in adult animals. Morpholinos are effective, powered alternatives to gamma radiation. Faxitron stretch cells in monolayer as well creating and specific, stable and non-toxic. Backed by Ph.D.- provides safe and reliable biological X-ray stretching 3D cell-seeded constructs. Flexcell level customer support, Gene Tools designs and irradiation, bringing the core lab to your lab. offers microscopy devices for viewing real-time synthesizes Morpholinos and offers cytosolic response. delivery options. Fibercell Systems, Inc. 527 905 West 7th Street Suite 334 Fluicell AB 1036 GeneCopoeia, Inc. 835 Frederick, MD 21701 Hugo Grauers Gata 3B 9620 Medical Center Drive Phone: (301) 865-6374 Gothenburg, SE-411 33 Sweden Suite 101 www.fibercellsystems.com www.fluicell.com Rockville, MD 20850 Phone: (301) 762-0888 FiberCell Systems supplies hollow fiber Fluicell AB develops and provides the first lab- www.genetex.com bioreactors for easy cell culture scale-up with on-a-tip tool, the BioPen, enabling unparalleled 100X productivity. Applications include antibody control of the local environment about individual GeneCopoeia products include largest collection and protein production, endothelial cell culture, cells, or surface regions, without contaminating of ready-to-express clones for ORF cDNA, stem cell culture and exosome production. CDM any other region within the sample dish. Current shRNA, promoters, microRNA precursors, HD is a chemically defined serum replacement application areas are focused within; single- inhibitors and microRNA 3’ UTR targets; lentiviral optimized for hollow fiber cell culture. A better way cell biology, organelle biology, enzymology, clones, packaging kits and pre-made lentivirus; to grow cells. pharmacology, tissue physiology and bioprinting. recombinant proteins and antibodies; qPCR products and Genome editing (CRISPR, TALEN/ Fine Science Tools 1112 Funakoshi Co., Ltd. 420 TALE-TF) tools. 373-G Vintage Park Drive 9-7 Hongo 2-Chome Foster City, CA 94404 Bunkyo-ku GeneTex 1106 Phone: (800) 521-2109 Tokyo, 113-0033 Japan 2456 Alton Parkway finescience.com Phone: +81-3-5684-6296 Irvine, CA 92606 www.funakoshi.co.jp Phone: (949) 553-1900 Fine Science Tools™ offers more than 900 www.genetex.com high-quality European surgical and microsurgical Funakoshi Co., Ltd. distributes research reagents instruments for research scientists and other and instruments for researchers in the life science GeneTex produces high-quality antibodies and professionals. Whatever you need – spring fields since its foundation. Our mission is to research reagents validated through extensive scissors, forceps, scalpels and more – we provide products to broad range of customers research, development and testing. GeneTex carry only the best. Visit us for a free copy of throughout our world. currently offers more than 57,000 products our complete catalog, or order online at www. covering 11 different research areas. Our products finescience.com. Garland Science 1216 have earned the trust of the research community 711 Third Avenue for their quality and reliability for over 15 years. Finger Lakes Instrumentation LLC 1113 8th Floor 1250 Rochester St New York, NY 10017 Genetic Engineering & Biotechnology News 1001 Lima, NY 14485 Phone: (917) 351-7138 140 Huguenot Street Phone: (585) 624-3760 www.garlandscience.com 3rd Floor www.flicamera.com New Rochelle, NY 10801 Please visit us at Booth 1216 to examine the Phone: (914) 740-2180 MicroLine cooled CCD cameras and High Speed new Sixth Edition of Molecular Biology of the Cell www.genengnews.com Filter Wheels (23 msec change time) under and the Fourth Edition of Essential Cell Biology, MicroManager / ImageJ software control. The both by Alberts et al. Also, browse our other Genetic Engineering and Biotechnology News compact deep-cooled MicroLine is a favorite publications such as The Biology of Cancer by (GEN) is the longest-running, most widely read, for both OEM and Research Labs for low-light Robert A. Weinberg. and largest circulated global biotechnology applications. MicroLines support over 40 different news publication. Published 21 times a year and CCDs including Sony ICX694 (6MP) and ON Semi GE Healthcare 325 recently redesigned to focus on Biobusiness, KAI-08050 (8MP). 800 Centennial Avenue OMICS, Drug Discovery, Bioprocessing, and PO Box 1327 Translational Medicine, GEN reports on key Fitzgerald Industries International 429 Piscataway, NJ 08854 news developments and technology trends in the 30 Sudbury Road, Suite 1A North Phone: (732) 457-8111 bioindustry. Acton, MA 01720 www.ge.com Phone: (978) 371-6446 www.fitzgerald-fii.com GE Healthcare provides expertise and tools for a wide range of applications, including basic Fitzgerald is a manufacturer and supplier of research of cells and proteins, drug discovery Primary and Secondary Antibodies, purified research, and tools to support large-scale Proteins, ultra-sensitive ELISA Kits and a manufacturing of biopharmaceuticals. By broad range of biological reagents. Our product combining our knowledge, talent, and resources, ranges span multiple research areas such as we deliver innovative products and solutions that Cell Biology, Cancer, Neuroscience, Infectious help our customers achieve their goals. Disease, Cardiac Markers, Cytokines/Growth Factors, and much more.
146 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting EXHIBITORS
Getson & Schatz, P.C. 728 Human Frontier Science Program 1321 Infinite Trading Inc. 1520 230 S. Broad Street 12 quai Saint-Jean 1810 E. Sahara Ave. Suite 1001 Strasbourg, 67080 France Suite 1482 Philadelphia, PA 19102 www.hfsp.org Las Vegas, NV 89104 Phone: (215) 520-4000 http://www.click4immigration.com/ The Human Frontier Science Program is an Digital massagers will be on display. international program of research support based We are an immigration law firm in Philadelphia, in Strasbourg, France. Its aims are to promote Innova Biosciences 1317 Pennsylvania, offering conference travel awards. intercontinental collaboration and training in Babraham Hall We proudly represent scientific researchers cutting-edge, interdisciplinary research focused on Babraham in filing green card petitions in the EB-1A the life sciences. Cambridge, CB22 3AT UK extraordinary ability aliens, EB-1B outstanding Phone: 0044-1223-496170 professors and researchers, and national interest Huygens Software - SVI 503 www.innovabiosciences.com waiver categories. We also represent companies Laapersveld 63 filing H-1B visa petitions for immigrant employees. Hilversum, 1213VB Netherlands Innova Biosciences is a rapidly growing, dynamic Phone: 011-31-3564-2162 business based in Cambridge, UK. At the core GORYO Chemical, Inc. 1003 www.svi.nl/FrontPage of Innova’s business are bioconjugation and Hokudai Business-Spring nanoparticle technologies including Lightning- 2F, Kita 21 Nishi 12-2, Kita-ku Huygens Software (SVI) is renowned for its Link®, InnovaCoat® and Thunder-Link® Sapporo, 001-0021 Japan excellent deconvolution and noise removal for brands - easy to use kits which overcome many http://goryochemical.com/ ALL types of light-microscopic images (WF, CF, of the problems associated with traditional SP, MPh, STED, SPIM). Be sure to analyse right bioconjugation. GORYO Chemical, Inc. provides novel fluorescent and apply Huygens GPU-driven deconvolution probes for cellular analysis, assay, and imaging. first. Also for Colocalization, Object Analysis Innovative Cell Technologies 1329 ProteoGREEN™-gGlu can detect only tumor and Tracking, Stitching, Big data processing, 6790 Top Gun St in ex-vivo. AcidiFluor™ ORANGE has high S/N Corrections Chromatic-Shifts, Cross-Talk, Ste. 1 ratio on acidic state. Our new fluorescent probe instability. San Diego, CA 92121 can detect cancer for fluorescent image guided www.accutase.com surgery at pre-clinical and clinical trials in Japan. ibidi USA Inc. 1121 510 Charmany Drive, Suite 268 ICT manufactures and sells Accutase and Greiner Bio-One 620 Madison, WI 53719-1235 Accumax cell detachment solutions. These 4238 Capital Drive Phone: (608) 845-1502 products are direct replacements for trypsin and Monroe, NC 28110 www.ibidi.com collagenase with superior viability and higher Phone: (800) 884-4703 cell recovery. They work for all types of stem cell www.gbo.com ibidi provides solutions for bio-microscopy. passaging and spheroid dissociation. Stop by our Imaging slides and dishes offer unique booth for a free sample. Setting the industry standard with innovative solutions for angiogenesis, chemotaxis, life science consumables, Greiner Bio-One wound healing, shear stress and flow assays, Institut Curie 1535 manufactures for tissue culture, molecular biology, immunofluorescence and cell culture. ibidi also 26, rue d’Ulm immunology and HTS. Featuring AutoFlask™ for offers specialized equipment for live cell imaging, Training Unit automated cell-culture, Light Protection Tubes such as heating and incubation systems, and flow Paris, 75005 France for light-sensitive reactions, Shorty Pipettes ideal pump systems to perform long-term flow assays. www.curie.fr for benchtop-hoods, ThinCert™ TC inserts for cell modeling, migration/invasion, and µClear®, iBiology 1224 Institut Curie’s 3 missions: Hospital / Research / lumox™, and SensoPlates™ for imaging/HCS. University of California, San Francisco Training 4 axes of research: -Biology & chemistry San Francisco, CA 94158 of radiation, cell signaling & cancer -Development, Hamamatsu Corporation 313 www.ibiology.org cancer, genetics, & epigenetics -Integrative tumor 360 Foothill Road biology, immunology & environment -Multi-scale Bridgewater, NJ 08807 iBiology (www.ibiology.org) is a growing collection physics, biology & chemistry: molecules, cells, Phone: (908) 231-0960 of free online videos by scientists about research, tissues, & organisms Goal:Fight against cancer, www.hamamatsu.com discoveries and other topics in the life sciences. improve patient care Visit our booth to learn about our videos and Hamamatsu Corporation is the North American educator resources. This project is funded by ISS 436 subsidiary of Hamamatsu Photonics K.K. (Japan), NSF, NIGMS, and HHMI, and supported by the 1602 Newton Drive a leading manufacturer of devices for the ASCB and UCSF. Champaign, IL 61822 generation and measurement of infrared, visible, Phone: (217) 359-8681 UV light, and x-rays. These devices include Implen, Inc. 400 www.iss.com sCMOS cameras, EM-CCD cameras, and CCD 31194 La Baya Drive cameras for microscopy. The company also offers Suite 104 ISS manufactures research-grade detectors and image sensors. Westlake Village, CA 91362 spectrofluorometers (PC1, K2, Chronos) for Phone: (818) 748-6400 time-resolved and steady-state measurements Horizon Discovery 602 www.implen.com and the Alba line of single-molecule confocal 7100 Cambridge Research Park microscopes for live cells measurements using Waterbeach Implen is a privately held corporation that is a one- and multi-photon excitation. Applications Cambridge, CB25 9TL UK leading supplier of spectroscopy instruments and include Fluorescence Resonance Energy Phone: 011-44-1223-655580 consumables for the non-destructive analysis of Transfer (FRET), Fluorescence Lifetime Imaging www.horizondiscovery.com ultra low volume samples. The company focuses (FLIM), Fluorescence Fluctuation Spectroscopy on biological, chemical, and pharmaceutical (FCS, FCCS, PCH, scanning FCS, Number & Horizon combines long scientific heritage in laboratories in industry and research. Brightness). translational research with a precision gene- editing platform incorporating rAAV, CRISPR and ZFN technologies. Horizon supplies genetically- defined cell lines, in vivo models, gene-editing tools and services, custom cell line generation, molecular reference standards, and contract research services.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 147 EXHIBITORS
Jackson ImmunoResearch Laboratories, Inc. 529 Keystone Symposia on Molecular 1523 LifeSensors, Inc. 1124 872 West Baltimore Pike and Cellular Biology 271 Great Valley Parkway West Grove, PA 19390 P.O. Box 1630 Malvern, PA 19355 Phone: (800) 367-5296 160 U.S. Highway 6, Suite 200 Phone: (610) 644-8845 www.jacksonimmuno.com Silverthorne, CO 80498 www.lifesensors.com Phone: (970) 262-2692 Specializing in affinity-purified secondary www.keystonesymposia.org LifeSensors, Inc. is a biotechnology company antibodies (many adsorbed against other species) leading the development of innovative research conjugated with Alexa Fluor®, DyLight™, and Through a range of diversity initiatives, Keystone tools and services for the ubiquitin and ubiquitin- Cyanine fluorescent dyes; PerCP; and other Symposia’s Diversity in Life Science Programs like protein pathways. In addition, LifeSensors detection ligands. Other products include anti-IgG, (DLSP) actively promotes participation of provides prokaryotic and eukaryotic protein Light Chain specific for Western blotting after IP, underrepresented (UR) investigators using a expression platforms: SUMOpro and SUMOstar. Alexa Fluor® 680 and 790 for highly sensitive variety of professional development activities, These products are used worldwide in academia, Western blots. ISO 9001:2008 registered. including, but not limited to, the Keystone Fellows government institutions, and pharmaceutical and program; Early-Career Investigator Travel Awards; biotechnology companies. Jones & Bartlett Learning 1222 the ABRCMS Scholarship program, and the Peer- 5 Wall Street to-Peer mentoring program. Lipotype GmbH 1223 Burlington, MA 01803 Tatzberg 47 Phone: (978) 443-5000 Labroots Inc 1039 Dresden, 01307 Germany www.jblearning.com 18340 Yorba Linda Blvd. Phone: +49 351 796 5344 Suite 107 PMB 427 www.lipotype.com/ Jones & Bartlett Learning is a world-leading Yorba Linda, CA 92886 provider of instructional, assessment, and www.labroots.com Lipotype is a spin-off company from the Kai learning-performance management solutions Simons and Andrej Shevchenko labs of the Max- for the secondary education, post-secondary LabRoots is the leading scientific social Planck-Institute of Molecular Cell Biology and education, and professional markets. Our networking website and producer of educational Genetics in Dresden, Germany. Lipotype delivers educational programs and services improve virtual events and webinars. Contributing to the comprehensive, absolutely quantitative and learning outcomes and enhance student advancement of science through content sharing structural lipid analysis services for clinical and achievement by combining authoritative content capabilities, LabRoots is a powerful advocate in biological samples on a high-throughput scale. with innovative, proven, and engaging technology amplifying global networks and communities. applications. Logos Biosystems, Inc. 534 LabX Media Group 1315 Doosan Venturedigm Ste 930 JPK Instruments AG 341 478 Bay Street 415 Heungan-daero, Dongan-gu Colditzstraße 34-36 Midland, ON, L4R 1K9 Canada Anyang-si, Gyeonggi-do 431-755 Berlin, 12099 Germany Phone: (705) 528-6888 Republic of Korea www.jpk.com www.leica-microsystems.com Phone: 82-10-2226-7295 www.logosbio.com JPK Instruments is a world-leading manufacturer LabX Media Group is a leading provider of timely of nanoanalytic instrumentation - particularly and essential news, analysis, research, data and Logos Biosystems is dedicated to the Atomic Force Microscopes and optical tweezers. insights for members of the research / life science development and commercialization of innovative First applying AFM technology, JPK recognized community, and related fields in lab professional technologies to support the life science research the opportunities within nanotechnology for services and technology through its two community. Since its founding in 2008, Logos transforming life sciences and soft matter publications – The Scientist and Lab Manager. Biosystems has been developing a series of research which led to JPK’s success in uniting the automated systems and imaging instruments for worlds of nanotechnology tools and life science Leica Microsystems 913 laboratories engaging in research with a cellular applications 1700 Leider Lane and molecular emphasis. Buffalo Grove, IL 60089 KEYENCE Corporation 1115 Phone: (800) 248-0123 Lumencor, Inc. 1100 1100 North Arlington Heights www.leica-microsystems.com 14964 NW Greenbrier Parkway Itasca, IL 60143 Beaverton, OR 97006 Phone: (888) 539-3623 Leica Microsystems offers infinite possibilities in Phone: (503) 358-8145 www.keyence.com Cell Biology Research. The Leica DMi8 inverted www.lumencor.com microscope grows to meet your ever-changing KEYENCE Corporation is a world leader needs for live cell imaging. We will show widefield, Lumencor is a leading manufacture of lighting for in advanced microscopes for imaging and confocal, and super-resolution platforms including clinical instruments including microscopes. Unlike measurement applications. Our latest all-in- the Leica TCS SP8 with Digital Light Sheet traditional lamps, our bright, light engines are one fluorescence microscope, the BZ-X700, is imaging, new Leica EM ICE high-pressure freezer, energy efficient and mercury free. revolutionizing the way that researchers perform and more. experiments, allowing them to collect better data Matsunami Glass USA Inc. 937 and publish results more quickly. Stop by to see Lifeline Cell Technology 516 1971 Midway Lane and test a system for yourself. 8415 Progress Dr Suite K Suite T Bellingham, WA 98226 Frederick, MD 21701 www.matsunami-usa.com www.lifelinecelltech.com Matsunami Glass USA is a leading manufacturer Lifeline Cell Technology provides Normal of superior quality laboratory glass products Human Cells and Optimized Serum-Free or including microscope slides, diagnostic slides, Low-Serum Media. The Lifeline Cell Culture specialty cover glass and bioscience products. Systems provide excellent in vitro models for many applications including drug screening, cytotoxicity studies, cancer research, wound healing studies, gene therapy, infectious disease studies, and regenerative medicine studies . www. lifelinecelltech.com
148 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting EXHIBITORS
MatTek Corporation 404 Mirus Bio LLC 607 Nacalai USA, Inc. 1313 200 Homer Ave 545 Science Drive 10225 Barnes Canyon Road Ashland, MA 01721 Madison, WI 53711 Suite A103 www.mattek.com Phone: (888) 530-0801 San Diego, CA 92121 www.mirusbio.com Phone: (858) 404-0403 MatTek Corporation is an in vitro life science https://www.nacalaiusa.com/ company providing innovative products and For 20 years, Mirus has led the way in gene assays. MatTek’s product portfolio includes delivery innovation. With the introduction of Nacalai USA strives to bring stringently quality- microphysiological 3D tissue models, primary CHOgro® Expression System, a transient controlled biochemicals and research tools from human cells & media and glass bottom dishes & transfection platform that produces high protein Japan. We provide the highest quality research multiwell plates offering the optics of glass in a titers, we expand our expertise in nucleic products for the Life Science such as Molecular petri dish ideal for fixed or live cell imaging. acid delivery and look to enhance output in Biology and Proteomics. We also provide biotherapeutic protein production, lentivirus Cosmosil brand HPLC columns including specialty Meso Scale Discovery 1417 production and genome editing. columns such as the Cholester (cholesteryl group) 1601 Research Blvd and core-shell columns. Rockville, MD 20850 MO BIO Laboratories, Inc. 321 Phone: (240) 314-2600 2746 Loker Avenue West nanoAnalytics GmbH 1035 www.meso-scale.com Carlsbad, CA 92010 Heisenbergstr. 11 www.mobio.com 48149 Münster, Germany Meso Scale Discovery (MSD®) is a leader in high Phone: +49(0)251.53406300 performance, multiplex-enabled biomarker assays MO BIO Laboratories, Inc. is a global leader in www.nanoanalytics.com for cytokines, toxicology, neurodegeneration and solutions for nucleic acid purification, offering metabolic disease research, used by leading innovative tools for research in molecular biology. nanoAnalytics presents the new cellZscope2, biopharmaceutical and academic researchers MO BIO’s line of soil, plant and microbial isolation a device for measuring the transepithelial/- in human and animal model studies. MSD’s kits are now the method of choice among endothelial electrical resistance (TER) and proprietary electrochemiluminescence (ECL) environmental and microbiology researchers capacitance of cell layers in standard cell culture platform delivers exceptional sensitivity, wide studying microbial DNA and RNA. inserts. cellZscope2 allows automated, long-term dynamic range and rapid turnaround. experiments under physiological conditions. It Montana Molecular 1320 is ideal for monitoring cell growth to confluence, Michigan Diagnostics, LLC 1513 810 N. Wallace Ave differentiation and studying the influence of drugs, 2611 Parmenter Blvd Suite B toxins etc. Royal Oak, MI 48073 Bozeman, MT 59715 Phone: (248) 435-4472 montanamolecular.com NanoEnTEK USA, Inc. 1126 www.michdiag.com 5627 Stoneridge Dr. Montana Molecular develops robust, genetically- Suite #304 We are one of the world’s leading manufacturers encoded fluorescent biosensors for live cell Pleasanton, CA 94588 of chemiluminescent diagnostic reagents and assay and imaging. Our products are used in www.nanoentek.com kits for research and clinical applications. We are drug discovery and to observe and measure cell committed to applying the highest quality and signaling processes. We enable researchers Nanoentek introduces the JuLI Stage, a compact, innovative solutions to service our clients’ needs. to measure biology at the molecular level in fully-automated, digital fluorescence imaging Please visit us at www.michdiag.com for more relevant cells including cardiomyocytes, neurons, analyzer for live-cell imaging from inside your information about our products and services. pancreatic islets and standard cell lines. incubator! Perfect for assay optimization, proliferation, cytotoxicity, cell migration studies, Mightex Systems 413 Motic Instruments 1038 HCS screening and other applications. Capable 2343 Brimley Road 130-4611 Viking Way of image stitching, producing time lapse movies, Suite 868 Richmond, BC, V6V 2K9 Canada and real time cell growth curve in GFP/RFP/DAPI/ Toronto ON, M1S 3L6 Canada Phone: (604) 303-9033 Brightfield formats. Phone: (416) 840-4991 www.motic-america.com www.mightex.com Nanolive SA 1322 Motic Instruments provides high quality Chemin de la Dent d’Oche 1a Mightex is a leading developer of advanced microscopes accessible to any budget. Motic’s Ecublens, Vaud 1024 Switzerland microscopy illumination sources including refined microscope design combines high www.nanolive.ch patterned and wide-field systems. Featured is the quality optics, ergonomic operation, and robust Polygon400 patterned illuminator which enables construction with a wide range of upgrades Nanolive developed the 3D Cell Explorer, the users to control light in 5 dimensions: 2-D Spatial, and accessories to complete its full line of Elite only holographic tomographic microscope that Intensity, Time, and Wavelength. Polygon is easy microscopes and digital imaging products for Life delivers quantitative cell tomography of living cells to use and simple to integrate into all commercial Science and Live Cell applications. (and tissues) in real-time based on the accurate microscopes. detection of the refractive index of each cell part. MTI-GlobalStem 517 The 3D Cell Explorer’s market launch will take Miltenyi Biotec, Inc. 1435 200 Perry Parkway place Monday night, 5:30 pm, in Theater 2. 2303 Lindbergh Street Suite 1 Auburn, CA 95602 Gaithersburg, MD 20877 Nanomedical Diagnostics Inc. 320 Phone: (530) 887-5311 www.globalstem.com 6185 Cornerstone Court Suite 110 www.miltenyibiotec.com San Diego, CA 92121 GlobalStem develops high-quality, standardized www.nanomedicaldiagnostics.com Miltenyi Biotec’s mission is to improve scientific stem cell reagents and innovative technologies understanding and medical progress. We provide for life science and neurobiology research. Our Based in San Diego, Nanomed is a biotech products and services that advance biomedical products include primary and iPSC-derived neural company developing and commercializing research and cellular therapy. Honoring this cells, neuronal culture media & supplements, bioelectronics for use in health care and life mission drives our commitment to support the pluripotent stem cells and stem cell culture media, science R&D. Our product, AGILE Research, is translation of basic research into therapy in the feeder cells, and transfection reagents for hard-to- a new label-free, quantitative, high-throughput areas of immunology, cancer, neuroscience and transfect cells. biosensor that is easy to use, portable, and stem cell biology. affordable. Stop by for a free beer and learn more!
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 149 EXHIBITORS
NanoSurface Biomedical 1221 New England Biolabs 521 Olympus 813 4005 15th Ave NE 240 County Road 48 Woerd Ave. Apt. 703 Ipswich, MA 01938 Waltham, MA 02453 Seattle, WA 98105 Phone: (978) 380-7352 Phone: (800) 446-5967 nsurfacebio.com www.neb.com www.olympus-lifesciencescom
NanoSurface Biomedical’s versatile biomimetic NEB, a leader in the discovery and production of Olympus Scientific Solutions Americas, a part cultureware technologies closely mimic the tools for molecular biology, offers a wide selection of the global Olympus life science and industrial topography of cellular micro-environments. Our of reagents for PCR, cloning, expression and instrumentation business, delivers solutions for cultureware products feature anisotropically purification. In addition to enzymes for DNA work, clinical, educational, and research microscopes, nanofabricated substrata (ANFS), which describes NEB offers expression systems, enzymes for nondestructive testing equipment, and analytical the parallel array of polymer ridges and grooves protein modification,competent cells, and tools for instruments. Designed to contribute to the safety, covering the culture area of these products. epigenetics research. security, quality and productivity of society, These groundbreaking tools raise the bar for Olympus strives to enhance people’s lives every physiological relevance in vitro. NIH Center for Scientific Review 1237 day. 6701 Rockledge Drive NASA Ames Research Center 1338 Room 1118 On-Chip 724 Mail Stop 247-9 Bethesda, MD 20892 7098 Miratech Dr. Ste 100 Moffett Field, CA 94035 Phone: (301) 435-1115 San Diego, CA 92121 www.nasa.gov www.csr.nih.gov www.on-chipbio.com
NASA space life sciences executes high CSR will be sharing exhibitor space with NIH/ Cryocell system-Programmable Deep Freezer has quality, high value research and application NIGMS for the 2015 ASCB Meeting in San a compact design for desktop use as well as the activities developing technologies that will allow Diego, CA, to display CSR publications/posters functionality of refrigeration to -80?. It freezes your humans to travel safely and productively in the & network throughout the meeting. The exhibit important cells while keeping high viability rate in environment of space. Come learn about grant space will be located in the Learning Center, a short time without using additional refrigerants. funding opportunities for spaceflight research and which is the networking hub of the meeting. Setting custom freezing programs is available. postdoctoral fellowships. Nikon Instruments Inc. 701 One World Lab 1314 National Institute of 1235 1300 Walt Whitman Road 6450 Lusk Blvd. General Medical Sciences Melville, NY 11747 Suite E103 45 Center Drive Phone: 1-800-52-NIKON San Diego, CA 92121 MCS 6200 www.nikoninstruments.com Phone: (855) 695-5227 Bethesda, MD 20892 www.oneworldlab.com Phone: (301) 496-7301 Nikon debuts N-STORM 4.0 for live-cell STORM www.nigms.nih.gov and N-SIM-E, the personal super-resolution One World Lab exclusively represents dozens system; a new LED-based tissue culture of original manufacturers and provides over The National Institute of General Medical microscope TS2; and new intermediate-size 100,000 products in test size amounts to give Sciences (NIGMS) is one of the National inverted microscope, TS2R. Also showcasing a researchers the ability to create affordable custom Institutes of Health, the principal biomedical suite of modular illumination devices; solid-state panels of antibodies and other reagents that can research agency of the Federal Government. laser combiners; High Content imaging system; be rated and reviewed to crowd-source product NIGMS supports basic research that increases and A1Rsi Confocal and Multiphoton system. performance which creates additional savings for understanding of biological processes and lays the end-user. the foundation for advances in disease diagnosis, Nortis, Inc. 334 treatment and prevention. 17280 Woodinville-Redmond Rd NE Open Imaging, Inc. 1312 Woodinville, WA 98072 1030 El Monte Ave #12 Nature Publishing Group 1212 www.nortisbio.com/ Mountain View, CA 94040 75 Varick Street https://open-imaging.com 9th Floor Nortis is a biotech company that has developed New York, NY 10013 tissue-engineering techniques and organ-on- Open Imaging develops, distributes, and provides Phone: (212) 726-9200 chip systems for the creation of human tissue support services for µManager (Micro-Manager), www.nature.com microenvironments within small, disposable, the free and open source optical microscopy microfluidic chips. Our technology enables living image acquisition and device automation The NPG portfolio combines the excellence models of human organs that will drastically software platform. µManager supports a wide of Nature, its associated research and review accelerate the discovery and introduction of new range of microscopes and peripheral devices, journals, and leading academic and society therapies. provides an intuitive user interface to run complex journals in the life, physical and clinical sciences. experiments, and is highly extensible and Open access options are offered through Novogene Bioinformatics 437 customizable. the academic and society journals, Nature Technology Co., Ltd Communications and through a new fully open 17 F, Tower B, Jinma Building OriGene 636 access publication: Scientific Reports. No.38 Xueqing Road, Haidian District 9620 Medical Center Drive Beijing, China Suite 200 Neogen Corporation 1424 Phone: 86-15811045793 Rockville, MD 20850 944 Nandino Blvd. www.novogene.com/ Phone: (240) 620-0285 Lexington, KY 40511 www.origene.com www.neogen.com/ Okolab SRL 700 Via G. Di Prisco, 152 OriGene is the world’s leading supplier of genome Neogen’s Life Science products include ELISA Ottaviano, 80044 Italy wide products for biomedical and diagnostic test kits for multiple research applications, Phone: 011-39-081-806-2624 research. Its comprehensive offering includes immunoassay reagents, and ready-to-use www.oko-lab.com cDNA clones, proteins, antibodies, CRISPR, liquid substrates for microwell and membrane RNAi and tissues. OriGene’s flagship product, applications. Our research assays are able Okolab manufactures superior performance UltraMAB® monoclonal antibodies, is the only to detect levels of hormones, steroids, microscope incubators and temperature/humidity/ class of antibodies that have undergone validation prostaglandins, leukotrienes, thromboxanes, gas controllers. We’ll be displaying our new against over 10,000 human protein antigens. cyclic nucleotides, lipoxins, cytokines, growth and Multi-User Centralized Gas Blender ideal for core angiogenic factors. facility.
150 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting EXHIBITORS
Otto Trading, Inc. 1125 Photometrics 921 Polyplus-transfection 837 1921 Carnegie Ave Suite C 3440 East Britannia Drive Bioparc Santa Ana, CA 92705 Suite 100 850 Bd S Brant www.unimedmassager.com Tucson, AZ 85706 Illkirch, 67400 France Phone: (520) 889-9933 Phone: 011-33-390-406187 Manufacturer and distributor of hand-held portable www.photometrics.com www.polyplus-transfection.com digital massager, TENS unit. Photometrics is a premier designer and Polyplus-transfection SA is a biotechnology Pacific Immunology 1335 manufacturer of high-performance, state-of-the- company that develops, manufactures, markets 1672 Main St Ste E 171 art CMOS, EMCCD cameras, CCD cameras and and sells innovative solutions for the in vivo, Ramona, CA 92065 multichannel imaging systems. As the original in vitro and ex vivo delivery of nucleic acids www.pacificimmunology.com architect of the world’s first scientific-grade in research, bioproduction and therapeutics. EMCCD camera, Photometrics has supported life Polyplus-transfection supplies customers Pacific Immunology is a leading global antibody science research for decades, enabling significant with its proprietary range of DNA, siRNA and manufacturer based in San Diego, California scientific contributions and advancements oligonucleotide transfection reagents worldwide. | USA that specializes in the development of globally. Monospecific, Phosphospecific and Sequence- PromoCell GmbH 537 Defined Rabbit Recombinant Monoclonal PI (Physik Instrumente) 316 Sickingenstrasse 63/65 Antibodies. We’ve helped countless scientists Piezo Nano Positioning Heidelberg, 69126 Germany publish their research, develop diagnostic tests 16 Albert Street Phone: 011-49-6221-649-340 and determine targets for therapeutic applications. Auburn, MA 01501 www.promocell.com Phone: (508) 832-3456 PeproTech, Inc. 1421 www.pi-usa.us PromoCell is a premier manufacturer of cell 5 Crescent Avenue culture and cell biology products, including P.O. Box 275 Leading manufacturer of precision motion control human primary cells, stem and blood cells, cell Rocky Hill, NJ 08553 equipment, piezo motors, air bearing stages & culture media, cell analysis kits, cell transfection Phone: (800) 436-9910 hexapod parallel-kinematics for semiconductor reagents, and many more. You can count on our www.peprotech.com applications, photonics, bio-nano-technology high quality products backed by experienced and medical engineering. PI has been developing friendly technical customer service! Since 1988, PeproTech has grown into a global and manufacturing standard & custom precision enterprise manufacturing an extensive line of products with piezoceramic and electromagnetic ProteinSimple 1136 Recombinant Human, Murine and Rat Cytokines, drives for 4 decades. PI provides innovative, high- 3001 Orchard Parkway Animal-Free Recombinant Cytokines, Monoclonal quality solutions for OEM & research. San Jose, CA 95134 Antibodies, Affinity Purified Polyclonal Antibodies, Phone: (408) 510-5558 Affinity Purified Biotinylated Polyclonal Antibodies, PicoQuant Photonics North America, Inc. 500 www.proteinsimple.com ELISA Development Kits, Cell Culture Media 9 Trinity Dr Products and GMP Cytokines. West Springfield, MA 01089 Our goal is to make protein analysis simpler, more Phone: (413) 562-6161 quantitative and affordable. Our comprehensive PhalanxBio, Inc. 528 www.picoquant-usa.com portfolio of tools includes Simple Western systems 6150 Lusk Blvd that quantify protein expression and Biologics Suite B100 Instrumentation for time-resolved fluorescence systems that probe the structure and purity of San Diego, CA 92121 and single photon counting: fluorescence lifetime protein-based therapeutics. Phone: (650) 320-8669 systems (confocal microscopes, spectrometers), www.phalanxbiotech.com FLIM and FCS upgrade kits for laser scanning Proteintech Group Inc. 417 microscopes, picosecond/nanosecond pulsed and 2201 West Campbell Park Drive Phalanx Biotech’s expertise in Expression modulated diode lasers, PC modules for Time- Chicago, IL 60612 Profiling and Genomic Analysis stems from its Correlated Single Photon Counting, detectors. Phone: (312) 455-8498 excellence as an original design manufacturer of www.ptglab.com OneArray® gene expression microarrays. The Platypus Technologies, LLC 522 OneArray® service lab has a top notch technical 5520 Nobel Drive, Suite 100 Proteintech is the original manufacturer of over team to help advance your genomic research Madison, WI 53711 11,000 antibodies validated in WB and IHC on using NGS, microarray and qPCR services. Phone: (608) 237-1270 primary tissues and cell lysates. Whole protein www.platypustech.com antigens produce antibodies with unparalleled Phasefocus 435 high quality working in more species and Electric Works Platypus Technologies provides assays for applications. Locations (US, Europe, China, Sheffield Digital Campus quantitative analysis of 3D cell invasion and 2D Japan), all antibodies in stock, next-day delivery. Sheffield, S1 2BJ UK cell migration in 96-well and 384-well formats. Phone: 011-44-1142-966377 These Oris™ assays use exclusion zone QImaging 923 www.phasefocus.com technology to generate unambiguous, statistically 19535 56th Avenue robust data in real time. Assay results can be Suite 101 Phasefocus will exhibit an innovative microscopy acquired with plate readers, microscopes or high Surrey, BC, V3S 6K3 Canada system for label-free, extended live cell imaging. content imagers. Phone: (604) 530-5800 The system uses the technique of ptychography www.qimaging.com to deliver quantitative, high contrast images, PLOS: Public Library of Science 1244 enabling accurate segmentation and tracking of 1160 Battery Street QImaging designs and manufactures user- single cells and heterogeneous cell populations. Suite 100 friendly Scientific CMOS, CCD, EMCCD and San Francisco, CA 94111 micropublisher cameras for life science research. Phone: (415) 624-1200 Compact and easy-to-use, QImaging cameras https//:www.plos.org are affordable, feature-rich solutions that provide outstanding versatility, ease of use and reliability. PLOS (Public Library of Science) is a nonprofit Customers use QImaging cameras to perform Open Access publisher, innovator and advocacy quantitative image analysis and acquire high- organization dedicated to accelerating progress in resolution images for publication. science and medicine by leading a transformation in research communication. The PLOS suite of influential journals contain rigorously peer- reviewed Open Access research articles from all areas of science and medicine.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 151 EXHIBITORS
RayBiotech 940 RPMC Lasers, Inc. 1441 ScienCell Research Laboratories 1416 3607 Parkway Lane 203 Joesph Street 6076 Corte Del Cedro Suite 100 O’Fallon, MO 63366 Carlsbad, CA 92011 Norcross, GA 30092 Phone: (636) 272-7227 Phone: (760) 602-8549 Phone: (770) 729-2992 www.rpmclasers.com www.sciencellonline.com www.raybiotech.com RPMC Lasers, Inc. offers innovative Diode ScienCell Research Laboratories is an expanding RayBiotech, Inc. is the pioneer and industry leader Pumped Solid-State Lasers and Laser Diode biotechnology company whose mission is the of antibody and protein array technologies which Modules in wavelengths in the IR, visible, and research and development of cell and cell-related allow efficient, cost-effective detection of multiple near-UV spectrums with market-leading output products for experimental and therapeutic use. disease-related proteins from any liquid biological power. The DPSS lasers feature a monolithic ScienCell provides a variety of high quality normal sample. Our technologies hold great promise resonator technology that ensures true single- human and animal cells, cell culture media, for the discovery of disease mechanisms, novel frequency emission along with excellent power, reagents, growth supplements, DNA, RNA, and biomarkers, and drug targets. wavelength, and pointing stability. assay kits.
Reagency Pty Ltd 942 RURO Inc. 939 Seahorse Bioscience, Inc. 1512 PO Box 9242 321 Ballenger Center Drive 16 Esquire Rd South Yarra, Victoria 3141 Australia Suite 102 North Billerica, MA 01862 Phone: +61 (0) 43 109 1329 Frederick, MD 21703 www.seahorsebio.com http://reagency.co/ Phone: (888) 881-7876 www.ruro.com Seahorse Bioscience XF metabolic analyzers Reagency gives you rapid, anonymous access and stress test kits are the industry standard for to custom synthesis of the latest literature RURO, Inc. brings our partners the most modern measuring cell metabolism. XF Extracellular Flux compounds and custom antibodies production solutions for their information management needs. Analyzers enable the simultaneous measurement at discounted prices. You decide what we make, RURO systems decrease staff time requirements of the two major energy pathways of the cell - the cost is shared with other scientists. Complete for data entry, compilation, and reporting by a mitochondrial respiration and glycolysis – in live security guaranteed. We’re not a catalogue or third, and reduce user input errors by up to 97%. cells, in real time. fee-for-service company. We’re changing the way For sample management, LIMS or ELN, RURO is researchers buy reagents. Laboratory Information Bliss. SETA BioMedicals 423 2014 Silver Ct East Reveal Biosciences 1215 San Diego Blood Bank 525 Urbana, IL 61801 6195 Cornerstone Ct E Ste 110 3636 Gateway Center Ave www.setabiomedicals.com San Diego, CA 92121 Suite 100 www.revealbio.com San Diego, CA 92102 SETA BioMedicals produces, commercializes and www.sandiegobloodbank.org licenses proprietary fluorescent detection reagents Reveal Biosciences specializes in advanced (fluorescent dyes, probes, labels, tandems for tissue technologies. Our fully automated San Diego Blood Bank provides consented, high flow cytometry, and fluorescent standards) for facility and experienced scientists offer in situ quality blood-related products and services to life a broad range of applications in the biomedical, hybridization (ISH), immunohistochemistry (IHC), science researchers. For example, Clearsate™, pharmaceutical, and environmental research image analysis and digital pathology expertise. a human platelet lysate, is a highly effective cell fields. Our ISH technology combines a highly sensitive, growth media supplement for a variety of cell multiplexed mRNA assay with our proprietary lines, and a viable alternative to FBS. Stop by to SGI-DNA, A Synthetic Genomics, Inc. 1000 imaging software to provide quantitative ISH learn more. Company localization data. 11099 North Torrey Pines Rd. Suite 150 Sarstedt, Inc. 1029 La Jolla, CA 92037 Rockefeller University Press 1139 P.O. Box 468 http://sgidna.com/ 950 Third Avenue Newton, NC 28658 Floor 2 Phone: (800) 257-5101 SGI-DNA, a Synthetic Genomics, Inc. company, New York, NY 10022 www.sarstedt.com was founded in 2013 and is headquartered in Phone: (212) 327-7945 La Jolla, California. Building on the scientific www.rupress.org Sarstedt provides laboratory consumables and advancements and breakthroughs from leading equipment, including supplies for cell culture, PCR scientists such as J. Craig Venter, Hamilton The Rockefeller University Press is committed and molecular biology. Our new and improved cell Smith, and Dan Gibson, SGI-DNA provides to quality and integrity in scientific publishing. culture products are redesigned with input from powerful and versatile tools for DNA design and Our goal is to publish excellent science using the our customers for superior cell growth, ease of assembly. latest technologies. We carry out rigorous peer use, and traceability. Ask about FREE samples of review, applying the highest standards of novelty, these new flasks, dishes, and plates. Sino Biological Inc. 840 mechanistic insight, data integrity, and general 14 Zhong He Street, BDA interest. Science/AAAS 1128 Bejing, 100176 China 1200 New York Avenue, NW Phone: 4008909903 Royal Society Publishing 1236 Washington, DC 20005 www.sinobiological.com/ 6-9 Carlton House Terrace Phone: (202) 326-6417 London, SW1Y 5AG UK www.aaas.org Leading global biological solution specialist, http://royalsocietypublishing.org offering a comprehensive set of value-added and Since 1848, AAAS and its members have worked cost-effective premium quality solutions (services The Royal Society publishes three journals together to advance science and serve society. As and reagents) to accelerate life science research aimed at cell biologists. Open Biology publishes part of these efforts, AAAS publishes Science, a and biological product development worldwide. outstanding open access research and review multidisciplinary peer-reviewed journal, and offers articles; Philosophical Transactions B publishes programs focused on science policy, international topical theme issues; Royal Society Open Science cooperation, science education, diversity, and publishes high quality, open access research on career development for scientists. the basis of objective peer review. Find out more at booth 1236.
152 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting EXHIBITORS
SmarAct Inc 1025 Sutter Instrument Company 901 TESCAN USA Inc. 1020 2140 Shattuck Ave One Digital Dive 765 Commonwealth Dr. Ste 1103 Novato, CA 94949 Suite 101 Berkeley, CA 94704 Phone: (415) 883-0128 Warrendale, PA 15086 www.smaract.com www.sutter.com www.tescan-usa.com
SmarAct offers smallest high performance Introducing the IPA™ Integrated Patch Amplifier, TESCAN USA is a leading supplier in North nano-manipulators, compact positioning systems a new state-of-the-art instrument combining a America of Scanning Electron Microscopes,Light with micro- and nanometer resolution for micro low-noise patch clamp amplifier, onboard data Microscopes, and Focused Ion Beam and nano-world; advanced control systems and acquisition via high-speed USB connectivity, workstations. The quality, performance and micro-manipulating tools. SmarAct also offers and SutterPatch™, a comprehensive software reliability of our products are the foundation of our complete miniaturized manipulation systems package built on the foundation of IGOR Pro 7. business, serving customers in academia, industry and expertise for complex assembling tasks. See also the Lambda HPX-L5 high-output LED and the government sector. Featured Product: PicoScale Laser interferometer light source, and QUAD 4-axis micromanipulator. for highest demands in resolution accuracy and The Allen Institute for Cell Science 1120 compactness. Sysmex America, Inc 524 551 N. 34th Street 577 Aptakisic Road Seattle, WA 98103 Society for Neuroscience 1525 Lincolnshire, IL 60069 https://www.alleninstitutecellscience.org 1121 14th Street NW www.takasago-fluidics.com Suite 1010 Launched with a contribution from Paul G. Allen in Washington, DC 20005 Sysmex America, Inc. is a leader in laboratory 2014, the Allen Institute for Cell Science studies Phone: (202) 962-4000 solutions, including clinical diagnostics, the cell as an integrated system. The Institute will www.sfn.org automation and information systems. Serving produce novel visual, dynamic, predictive models laboratories for 40 years in more than 150 of the cell that will accelerate cell biology and The Society for Neuroscience is the world’s countries, Sysmex focuses on extending the biomedical research. largest organization of scientists and physicians boundaries of diagnostic science while providing devoted to understanding the brain and nervous the management information tools that make a The Company of Biologists 1220 system. Founded in 1969, SfN has nearly 38,000 real difference. Bidder Building members in more than 90 countries and 150 Station Road, Histon chapters worldwide. The Society publishes The Takasago Fluidic Systems 747 Cambridge, Cambs CB24 9LF UK Journal of Neuroscience and the open-access 66 Kakitsubata Phone: 011-44-1223-424430 journal eNeuro. Narumi-cho, Midori-ku www.biologists.com Nagoya, 458-8522 Japan Sony Biotechnology Inc 438 Phone: +81-52-891-2301 The Company of Biologists is a not for profit 1730 North First Street www.tandfonline.com publishing organisation dedicated to supporting San Jose, CA 95112 and inspiring the biological community through Phone: (408) 352-4256 Takasago Fluidic Systems offers comprehensive scientific journals, meetings and grants. The www.sonybiotechnology.com fluid control systems for tissue engineering, cell Company publishes five specialist peer-reviewed culture and regenerative medicine applications. journals: Development, Journal of Cell Science, Sony Biotechnology Inc. is dedicated to helping Exhibits include a portable medium exchange Journal of Experimental Biology, Disease Models the immunology community of scientists, system, an automated perfusion culture & Mechanisms and Biology Open. researchers, laboratory professionals, and system, a palmtop culture medium control institutions achieve the best scientific results system, embeddable disposable pumps, etc. Thermo Fisher Scientific 613 possible. By leveraging Sony’s comprehensive We specialize in customization, integration and 5791 Van Allen Way expertise in electronics innovation and design and miniaturization. Carlsbad, NY 92008 with our technological assets we are accelerating Phone: (800) 955-6288 development of next-generation cell analysis Taylor & Francis 1123 www.lifetechnologies.com systems. 530 Walnut Street, Suite 850 Philadelphia, PA 19106 Thermo Fisher Scientific is the world leader in St. Jude Children’s Research Hospital 1034 www.tandfonline.com serving science. Our mission is to enable our 262 Danny Thomas Place customers to make the world healthier, cleaner MS 276 Taylor & Francis is committed to the publication and safer. Through our Thermo Scientific, Memphis, TN 38105 of scholarly research and publishes a variety of Applied Biosystems, and Invitrogen brands, we Phone: (901) 595-2750 journals concerning the field of cell biology. Visit offer an unmatched combination of innovative www.stjude.org/postdoc us online at www.tandfonline.com or visit the technologies, purchasing convenience and Taylor & Francis Journals booth to learn about support. A non-profit biomedical research institution our products and services, and to request FREE in Memphis, Tennessee, where researchers sample copies. Tokai Hit Co., Ltd. 925 investigate the molecular basis of both normal and 306-1 Gendoji-cho diseased cellular processes. Our collaborative Tecan 1438 Fujinomiya-shi research environment is optimal for inter- 9401 Globe Center Dr. Shizuoka-ken, 418-0074 Japan disciplinary, translational research, and our Suite 140 Phone: 81-544-24-6699 postdoctoral fellows have access to state-of-the- Morrisville, NC 27560 www.tokaihit.com art and well-staffed core facilities. Visit our booth Phone: (919) 361-5200 to discuss postdoctoral opportunities. www.tecan.com Tokai Hit provides small Incubators that enable time-lapse-imaging on microscopes for Wide Strex 722 Tecan is a leading global provider of automated temperature range Heating/Cooling. Perfusion and 7098 Miratech Dr. Ste 100 workflow solutions for laboratories in the life Drug-administration. No more cage incubator is San Diego, CA 92121 sciences sector. Its clients include pharmaceutical required. It is efficient for confocal, fluorescence, www.strexcell.com and biotechnology companies, university SIM and TIRF, favored in Live-Cell-Imaging field. research, forensic and clinical diagnostic We also introduce Clear-Glass-Heaters to assist Cryocell system-Programmable Deep Freezer laboratories. your treatment of live specimens by producing has a compact design for desktop use as well as optimal temperature. the functionality of refrigeration to -80?. It freezes your important cells while keeping high viability rate in a short time without using additional refrigerants. Setting custom freezing programs is available.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 153 EXHIBITORS
Tucsen Photonics Co., Ltd 401 VitaCyte LLC 514 World Precision Instruments, Inc. 1007 6F No.1 Building Cai mao Clothing Zone 1102 Indiana Avenue 175 Sarasota Center Blvd 756# Qi’an Road.Gaishan Town, Cangshan Area Indianapolis, IN 46202-3206 Sarasota, FL 34240 Fuzhou, Fujian China www.vitacyte.com Phone: (941) 371-1003 www.tucsen.com www.wpiinc.com VitaCyte manufactures and sells defined Since 2006 Tucsen has been developing and collagenase and neutral protease enzymes, World Precision Instruments, Inc. (WPI) has been manufacturing progressively more sophisticated minimizing the need to pre-qualify lots prior to a provider of laboratory solutions for the Life cameras for application in life science imaging. In use for cell isolation. These products include a Sciences for nearly 50 years. Our Cell Biology mid-2015, Tucsen launched a range of sCMOS low cost, defined enriched collagenase (5 types); display features Live Cell Imaging incubation for cameras including a version featuring 90% purified enzyme mixtures for specific cell isolation Stagetop and Full Microscope enclosures, Gas quantum efficiency, establishing themselves as applications; and individually purified collagenase Mixers, Environment Controllers and our NEW a technology leader in the life sciences imaging and neutral protease enzymes. Biofluorometer for fluorescent imaging studies. market. VitaScientific 1213 Worthington Biochemical Corporation 628 Union Biometrica, Inc. 513 8400 Baltimore Ave., Suite 302 730 Vassar Avenue 84 October Hill Road College Park, MD 20740 Lakewood, NJ 08701 Holliston, MA 01746 www.vitascientific.com Phone: (732) 942-1660 Phone: (508) 893-3115 www.Worthington-Biochem.com www.unionbio.com VitaScientific is a vibrant biological company serving the life science research community. Worthington is an ISO9001-Certified primary Union Biometrica Large Particle Flow Cytometers Through our premier web portal, VitaScientific. supplier of Collagenases and other enzymes automate the analysis & dispensing of objects too com, we supply innovative lab equipment, for primary & stem cell isolation, proteomics, big/fragile for traditional flow cytometers. Samples supplies, reagents, and kits, such as the X-Clarity, molecular and cell biology research. New! Animal include large delicate cells (adipocytes, plant the first commercial electrophoretic tissue clearing Free Collagenases, STEMxyme® Collagenase/ protoplasts), cell clusters (EBs, islets), spheroids device on the market. Neutral Protease Blends, new Catalog and Cell (neurospheres, tumorspheres), hydrogel- Isolation/Tissue Guide are now available. Take encapsulated samples, and others. COPAS and VWR International, LLC 1037 our survey for a chance to win an iPAD! BioSorter instruments cover the full 10-1500µm 100 Matsonford Road size range. Radnor, PA 19087 ZenBio, Inc. 1135 www.vwr.com 3200 East Highway 54 UVP LLC 1114 Suite 100 2066 West 11th Street At VWR, we work hard to keep your research RTP, NC 27709 Upland, CA 91786 on the cutting edge by providing you with over 1 Phone: (919) 547-0692 Phone: (909) 946-3197 million products from best-in-class manufacturers. www.zyagen.com uvp.com Our product offering is continually expanding to include foremost manufacturers as well as our ZenBio is a leading global provider of cell- UVP LLC, An Analytik Jena Company, will exhibit collection of brands, providing quality products at based solutions and services to the life science, selection of gel/chemiluminescence imaging the right price. cosmetics, and personal care communities. systems including ChemiDoc-ItTS2 with touch Founded in 1995, it was a pioneer in adipose screen for streamlined image capture. UVP W.H. Freeman & Company 1214 derived stem cells. Our mission is to provide the manufactures ultraviolet lamps, PCR hoods, 41 Madison Avenue highest quality human cell systems, reagents and transilluminators, crosslinkers and hybridization New York, NY 10010 contract services to our research partners. ovens. UVP offers Analytik Jena products Phone: (212) 375-7154 including thermal cyclers, qPCR/standard PCR www.macmillanhighered.com Zyagen 941 systems, homogenization equipment and DNA/ 10225 Barnes Canyon Road RNA isolation/extraction kits. W.H. Freeman — a Macmillan Education Suite A205 Company — publishes high-quality textbooks San Diego, CA 92121 Vector Laboratories 1429 and digital tools for the education community www.zyagen.com 30 Ingold Road worldwide. Stop by our booth to review some of Burlingame, CA 94010 our outstanding titles and learn more about our For 15 years, Zyagen has been manufacturing Phone: (650) 697-3600 innovative LaunchPad media. and marketing quality life science products such www.vectorlabs.com as RNA, DNA, cDNA, Protein, tissue sections and Wiley 1238 antibodies. Zyagen also offer custom research U.S. based manufacturer of protein and nucleic 350 Main Street services in histology, biochemistry, and molecular/ acid labeling and detection systems since 1976. Malden, MA 02148 cell biology. Our products and services enable We make reagents of the highest quality for Phone: (201) 748-6000 scientists to accelerate exploration leading to immunohistochemistry, immunofluorescence, www.wiley.com discoveries that make life even better. in situ hybridization, blotting applications, ELISAs, neuronal tracing, glycobiology and flow Wiley is a global provider of knowledge and Zymo Research Corporation 1028 cytometry. Innovative products include Vectastain knowledge-enabled services that improve 17062 Murphy Avenue ABC Kits, ImmPRESS Polymer Reagents, and outcomes in areas of research, professional Irvine, CA 92614 VECTASHIELD Mounting Media. practice, and education. Visit www.wiley.com for Phone: (949) 679-1190 more information. https://www.zymoresearch.com Vision Engineering 742 570 Danbury Road Since 1994, Zymo Research has been offering New Milford, CT 06776 innovative, quality, and easy-to-use tools for www.isioneng.us Epigenetics research and DNA/RNA purification. As “The Epigenetics Company” Zymo Research Vision Engineering designs and manufactures is an industry leader in epigenetic product and ergonomic, “eyepieceless” stereo inspection service development. Our products are well microscopes, used by many in the cell biology known for their quality, affordability, efficiency, and field. Since its formation in 1958, Vision unparalleled technical and customer support. Engineering has become one of the world’s most innovative and dynamic microscope companies.
154 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting EXHIBITOR PRODUCT INDEX EXHIBITOR PRODUCT INDEX
B-Bridge International, Inc. Biological cultures Blood collecting Bio-Rad Laboratories Allele Biotechnology systems A Bio-Techne MatTek Corporation Sarstedt, Inc. Acrylamide Bioss, Inc. ScienCell Research EMD Millipore Cell Signaling Technology Laboratories Blood proteins VitaScientific Inc. Cosmo Bio Co., Ltd ZenBio, Inc. ZenBio, Inc.
EBioscience, An Affymetrix Adjuvants Company Biological stains and Blood separation media Pacific Immunology EMD Millipore indicators ZenBio, Inc.
Elabscience Biotechnology Abcam, Inc. Affinity purification kits Co., Ltd Cell Signaling Technology Books and journals EMD Millipore FiberCell Systems, Inc. Electron Microscopy AAAS Funakoshi Company, Ltd. Funakoshi Company, Ltd. Sciences BioTechniques Nacalai USA, Inc. Innova Biosciences ibidi, LLC EMBO, EMBO Press
LifeSensors, Inc. ScienCell Research LABX Media Group Affinity purified Miltenyi Biotec, Inc. Laboratories Labroots, Inc. antibodies Nacalai USA, Inc. VitaScientific, Inc. Nature Publishing Group Abbiotec Pacific Immunology ZenBio, Inc. The Company of Biologists Cell Signaling Technology Reagency Pty Ltd. Molecular Biology of the Cell Cytoskeleton, Inc. Sony Biotechnology, Inc. Biologicals CBE—Life Sciences EMD Millipore Vector Laboratories BioChain Institute, Inc. Education Elabscience Biotechnology VitaScientific, Inc. Reagency Pty Ltd. Co., Ltd. ScienCell Research Buffers Funakoshi Company, Ltd. Autoclaves Laboratories Cell Signaling Technology Jackson ImmunoResearch World Precision Instruments, Worthington Biochemical EMD Millipore Laboratories, Inc. Inc. Corporation Electron Microscopy Pacific Immunology ZenBio, Inc. Sciences Vector Laboratories Automated sample MO BIO Laboratories, Inc.
preparation workstation Bioreactors Nacalai USA, Inc. Agarose BioChain Institute, Inc. EMD Millipore ScienCell Research EMD Millipore TECAN FiberCell Systems, Inc. Laboratories MO BIO Laboratories, Inc. GE Healthcare ZenBio, Inc. VitaScientific, Inc. Automatic samplers
BioChain Institute, Inc. Biosenors Alkaline phosphatase World Precision Instruments, conjugated antibody Inc. EBioscience, An Affymetrix C Company Cameras- CCD Biotin-conjugated Innova Biosciences 3i Intelligent Imaging B antibodies Jackson ImmunoResearch Balances Innovations AAT Bioquest, Inc. Laboratories, Inc. VitaScientific, Inc. Andor Technology Abcam, Inc. Vector Laboratories Echo Laboratories Cell Signaling Technology Biochemical reagents Finger Lakes Instrumentation EBioscience, An Affymetrix Amino acids AAT Bioquest, Inc. LLC Company Funakoshi Company, Ltd. Abcam, Inc. GE Healthcare EMD Millipore Accurate Chemical & Hamamatsu Corporation Elabscience Biotechnology Amplifiers Scientific Corporation Mightex Systems Co., Ltd. Funakoshi Company, Ltd. EMD Millipore Nikon Instruments, Inc. Vector Laboratories Sutter Instrument Company Elabscience Biotechnology Olympus America
World Precision Instruments, Co., Ltd. Photometrics Biotinylated DNA probes Inc. LifeSensors, Inc. QImaging Biosearch Technologies, Inc. Nacalai USA, Inc.
Analytical services ScienCell Research Cameras- cooled CCD Biotinylated antibody Lipotype GmbH Laboratories 3i Intelligent Imaging AAT Bioquest, Inc. VitaScientific, Inc. Innovations Abbiotec Animal tissues Andor Technology Abcam, Inc. BioChain Institute, Inc. Biochemicals Finger Lakes Instrumentation Bioss, Inc. AAT Bioquest, Inc. LLC Cell Signaling Technology Antibodies Abcam, Inc. Hamamatsu Corporation EBioscience, An Affymetrix Abbiotec Worthington Biochemical Nikon Instruments, Inc. Company Abcam, Inc. Corporation Olympus America EMD Millipore Accurate Chemical & ZenBio, Inc. Photometrics Elabscience Biotechnology Scientific Corporation QImaging Co., Ltd. Allele Biotechnology Bioimaging analyzer UVP LLC Jackson ImmunoResearch Atlas Antibodies AB Sony Biotechnology, Inc. Laboratories, Inc. Union Biometrica, Inc. Vector Laboratories
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 155 EXHIBITOR PRODUCT INDEX
Cameras- digital Cell culture carrier Reagency Pty Ltd. Conferences systems ScienCell Research ScienCell Research Labroots, Inc. 3i Intelligent Imaging Laboratories Laboratories SBCF (French Society for Innovations Cell Biology) Echo Laboratories Cell culture media Chemiluminescence Hamamatsu Corporation B-Bridge International, Inc. reagents Conjugation of Nikon Instruments, Inc. BioChain Institute, Inc. Cell Signaling Technology antibodies Olympus America EMD Millipore Elabscience Biotechnology Abcam, Inc. QImaging FiberCell Systems, Inc. Co.,Ltd Cell Signaling Technology Funakoshi Company, Ltd. NEOGEN CORPORATION Innova Biosciences Cameras- specialty CCD MatTek Corporation Nacalai USA, Inc. SETA BioMedicals 3i Intelligent Imaging Miltenyi Biotec, Inc. Vector Laboratories Innovations Mirus Bio LLC Constant temperature Echo Laboratories Nacalai USA, Inc. Chromatograph tubes or circulator Finger Lakes Instrumentation ScienCell Research columns VitaScientific, Inc. LLC Laboratories Nacalai USA, Inc. Hamamatsu Corporation ZenBio, Inc. Contract and R&D Olympus America Chromatographs- affinity- Cellecta, Inc. Photometrics Cell cultures matrices PhalanxBio, Inc. QImaging B-Bridge International, Inc. EMD Millipore BioChain Institute, Inc. Control serums Carboyhdrates Cosmo Bio Co., Ltd. Chromatographs- liquid- ZenBio, Inc. Accurate Chemical & Funakoshi Company, Ltd. accessories Scientific Corporation MatTek Corporation EMD Millipore Cross-linkers Mirus Bio LLC UVP LLC Cell biology products Polyplus-transfection Chromatographs- liquid- AAT Bioquest, Inc. SGI-DNA, A Synthetic high pressure Cryogenic vials Allele Biotechnology Genomics, Inc. Company EMD Millipore Sarstedt, Inc. Applied BioPhysics, Inc. ScienCell Research VitaScientific, Inc. B-Bridge International, Inc. Laboratories Chromatography columns Bio-Rad Laboratories ZenBio, Inc. and tubes Culture apparatus Biosearch Technologies, Inc. Bio-Rad Laboratories NanoAnalytics GmbH Cell Signaling Technology Cell membrane labeling GE Healthcare Takasago Fluidic Systems Cosmo Bio Co., Ltd. Cell Signaling Technology Nacalai USA, Inc. Cytoskeleton, Inc. Culture media Echo Laboratories Cell separation medium Chromatography Allele Biotechnology MatTek Corporation Accurate Chemical & systems B-Bridge International, Inc. NanoAnalytics GmbH Scientific Corporation Bio-Rad Laboratories Funakoshi Company, Ltd. Nanolive SA GE Healthcare MatTek Corporation Reagency Pty Ltd. Cell separator MO BIO Laboratories, Inc. Seahorse Bioscience, Inc. B-Bridge International, Inc. Chromogenic substrates ScienCell Research Sarstedt, Inc. Miltenyi Biotec, Inc. Vector Laboratories Laboratories ScienCell Research ZenBio, Inc. Laboratories Cell sorters Cloning vectors Sony Biotechnology, Inc. Bio-Rad Laboratories B-Bridge International, Inc. Cultured cells VitaScientific, Inc. Beckman Coulter EMD Millipore Allele Biotechnology ZenBio, Inc. EMD Millipore Funakoshi Company, Ltd. B-Bridge International, Inc. Miltenyi Biotec, Inc. GeneCopoeia, Inc. BioChain Institute, Inc. Cell culture apparatus Sony Biotechnology, Inc. ScienCell Research EMD Millipore Union Biometrica, Inc. Colloidal gold Laboratories Electron Microscopy complexes ZenBio, Inc. Sciences Centrifugation media and Innova Biosciences FiberCell Systems, Inc. blood separation media Custom PCR service ibidi, LLC Axis-Shield PoC Colony counters B-Bridge International, Inc. Logos Biosystems, Inc. Cosmo Bio Co., Ltd UVP LLC ZenBio, Inc. Nacalai USA, Inc. NanoAnalytics GmbH Centrifuge tubes Competent cells Custom antibody Nanolive SA Sarstedt, Inc. Allele Biotechnology Abcam, Inc. Okolab SRL VitaScientific, Inc. New England Biolabs Allele Biotechnology ScienCell Research VitaScientific, Inc. Cell Signaling Technology Laboratories Centrifuges Elabscience Biotechnology VitaScientific, Inc. Beckman Coulter Computer-3-D Co., Ltd. VitaScientific, Inc. reconstruction Pacific Immunology Cell culture bags Nikon Instruments, Inc. Funakoshi Company, Ltd. Chemicals Custom peptides EMD Millipore Computers Abbiotec Funakoshi Company, Ltd. Institut Curie LifeSensors, Inc. Nacalai USA, Inc. Nikon Instruments, Inc. Pacific Immunology
156 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting EXHIBITOR PRODUCT INDEX
Custom screening EMD Millipore Diagnostic aids ScienCell Research BioChain Institute, Inc. Funakoshi Company, Ltd. ScienCell Research Laboratories Cytoskeleton, Inc. MO BIO Laboratories, Inc. Laboratories Scientific Volume Imaging LifeSensors, Inc. ScienCell Research ZenBio, Inc. Laboratories Diagnostic monoclonal Electrochemical antibodies instrumentation Custom synthesis DNA sequencer Allele Biotechnology UVP LLC AAT Bioquest, Inc. Novogene Bioinformatics Innova Biosciences Biosearch Technologies, Inc. Technology Co., Ltd. Electrodes GORYO Chemical, Inc. Diagnostic test kits World Precision Instruments, SETA BioMedicals DNA synthesis AAT Bioquest, Inc. Inc. SGI-DNA, A Synthetic Innova Biosciences Cuvettes- plastic Genomics, Inc. Company NEOGEN CORPORATION Electron microscopes Sarstedt, Inc. ScienCell Research Nikon Instruments, Inc. VitaScientific, Inc. DNA vectors Laboratories EMD Millipore Electrophoresis Cytokines SGI-DNA, A Synthetic Dialysis equipment accessories Bio-Rad Laboratories Genomics, Inc. Company EMD Millipore GE Healthcare Funakoshi Company, Ltd. Innova Biosciences Miltenyi Biotec, Inc. Data acquisition Diamond knives Nacalai USA, Inc. NEOGEN CORPORATION 3i Intelligent Imaging Electron Microscopy Nacalai USA, Inc. Innovations Sciences Electrophoresis cells Applied BioPhysics, Inc. World Precision Instruments, Nacalai USA, Inc. Echo Laboratories Inc. Nikon Instruments, Inc. Electrophoresis D Dispenser- automatic equipment DNA assay kits Data acquisition TECAN Applied BioPhysics, Inc. BioChain Institute, Inc. equipment Union Biometrica, Inc. Bio-Rad Laboratories ScienCell Research 3i Intelligent Imaging GE Healthcare Laboratories Innovations Dispensers- microliter and Logos Biosystems, Inc.
Echo Laboratories injection Nacalai USA, Inc. DNA assay system NanoAnalytics GmbH World Precision Instruments, Andor Technology Nikon Instruments, Inc. Inc. Electrophoresis media- ScienCell Research Union Biometrica, Inc. gels Laboratories Dissecting instruments MO BIO Laboratories, Inc.
Data analysis software Fine Science Tools Nacalai USA, Inc. DNA concentrators 3i Intelligent Imaging World Precision Instruments, Nacalai USA, Inc. Innovations Inc. Electrophoresis size
Andor Technology markers DNA isolation kits and DRVision Technologies LLC Dissecting microscopes Nacalai USA, Inc. supplies ibidi, LLC World Precision Instruments, B-Bridge International, Inc. Lipotype GmbH Inc. Electrophoresis- high BioChain Institute, Inc. Nikon Instruments, Inc. voltage EMD Millipore Photometrics Dissolved oxygen meter Nacalai USA, Inc. Funakoshi Company, Ltd. QImaging World Precision Instruments, MO BIO Laboratories, Inc. Sutter Instrument Company Inc. Electrophysiological Nacalai USA, Inc. instruments UVP LLC Data processing Dyed particles Olympus America
systems AAT Bioquest, Inc. Sutter Instrument Company DNA libraries DRVision Technologies LLC World Precision Instruments, B-Bridge International, Inc. Dyes and labeling Inc. BioChain Institute, Inc. Density gradient media reagents Novogene Bioinformatics Axis-Shield PoC AAT Bioquest, Inc. Electroporation system Technology Co., Ltd GE Healthcare Cell Signaling Technology VitaScientific, Inc. SGI-DNA, A Synthetic Cytoskeleton, Inc. World Precision Instruments, Genomics, Inc. Company Detection systems Innova Biosciences Inc.
3i Intelligent Imaging Sony Biotechnology, Inc. DNA modification Innovations Vector Laboratories Elisa plates/strips reagents EMD Millipore B-Bridge International, Inc. Mirus Bio LLC Nacalai USA, Inc. Bio-Rad Laboratories
ScienCell Research Cell Signaling Technology DNA probes Laboratories EMD Millipore AAT Bioquest, Inc. Vector Laboratories E Elabscience Biotechnology Biosearch Technologies, Inc. Educational material Co., Ltd. iBiology Detectors- light Innova Biosciences DNA purification kits Jones & Bartlett Learning 3i Intelligent Imaging LifeSensors, Inc. B-Bridge International, Inc. Keystone Symposia Innovations NEOGEN CORPORATION BioChain Institute, Inc. Labroots, Inc. Hamamatsu Corporation ScienCell Research Laboratories
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 157 EXHIBITOR PRODUCT INDEX
VitaScientific, Inc. Enzymes Flow cytometry reagents ISS, Inc. B-Bridge International, Inc. AAT Bioquest, Inc. KEYENCE CORPORATION Elisa testing BioChain Institute, Inc. Abcam, Inc. Olympus America BioChain Institute, Inc. Bio-Techne BD Biosciences Cell Signaling Technology EMD Millipore Beckman Coulter Fluorescent antibody Elabscience Biotechnology Funakoshi Company, Ltd. Cell Signaling Technology Abbiotec Co., Ltd. LifeSensors, Inc. EMD Millipore Bioss, Inc. Innova Biosciences Nacalai USA, Inc. Elabscience Biotechnology Cell Signaling Technology LifeSensors, Inc. ScienCell Research Co., Ltd. EMD Millipore NEOGEN CORPORATION Laboratories Innova Biosciences Innova Biosciences VitaCyte LLC Miltenyi Biotec, Inc. Jackson ImmunoResearch Eliza plates- specialty VitaScientific, Inc. SETA BioMedicals Laboratories, Inc. LifeSensors, Inc. Worthington Biochemical Sony Biotechnology, Inc. LifeSensors, Inc. Corporation Vector Laboratories Miltenyi Biotec, Inc. Employment SETA BioMedicals SBCF (French Society for Expression vectors Flow cytometry Vector Laboratories Cell Biology) EMD Millipore standards VitaScientific, Inc. ScienCell Research LifeSensors, Inc. BD Biosciences Laboratories Sony Biotechnology, Inc. Fluorescent particles St. Jude Children’s Research Extracellular matrix AAT Bioquest, Inc. Hospital products Fluorescence filter sets Allele Biotechnology B-Bridge International, Inc. Chroma Technology Corp Environmental chambers Funakoshi Company, Ltd. Echo Laboratories Fluorometers ibidi, LLC ZenBio, Inc. Nikon Instruments, Inc. ISS, Inc. Nikon Instruments, Inc. Olympus America World Precision Instruments, Okolab SRL Sutter Instrument Company Inc. UVP LLC World Precision Instruments, F Fluorescence microscopy Freezer Fetal bovine Inc. lamp unit BINDER, Inc. EMD Millipore 3i Intelligent Imaging FiberCell Systems, Inc. Enzyme immunoassay Innovations ScienCell Research reagents/kits Chroma Technology Corp Laboratories B-Bridge International, Inc. CoolLED Ltd G ZenBio, Inc. Gel electrophoresis Cell Signaling Technology Echo Laboratories equipment Elabscience Biotechnology GE Healthcare Film processing Accurate Chemical & Co., Ltd. Nikon Instruments, Inc. supplies Scientific Corporation Jackson ImmunoResearch Sutter Instrument Company EMD Millipore Bio-Rad Laboratories Laboratories, Inc. GE Healthcare LifeSensors, Inc. Fluorescence reagents Filter bottom plates NEOGEN CORPORATION AAT Bioquest, Inc. EMD Millipore Gel extraction kit ScienCell Research Allele Biotechnology B-Bridge International, Inc. Laboratories Biosearch Technologies, Inc. Filter membranes MO BIO Laboratories, Inc. Vector Laboratories Cell Signaling Technology EMD Millipore GORYO Chemical, Inc. VitaScientific, Inc. Gene cloning and expression Enzyme inhibitors Innova Biosciences products Cell Signaling Technology Montana Molecular Filter paper B-Bridge International, Inc. LifeSensors, Inc. SETA BioMedicals GE Healthcare Cellecta, Inc. MO BIO Laboratories, Inc. Sony Biotechnology, Inc. LifeSensors, Inc. Nacalai USA, Inc. Vector Laboratories Filter pipet tips SGI-DNA, A Synthetic Reagency Pty Ltd. Sarstedt, Inc. Genomics, Inc. Company ScienCell Research Fluorescence systems
Laboratories 3i Intelligent Imaging Filters Genomic DNA purification Innovations Chroma Technology Corp kit Enzyme reagents Bruker Corporation EMD Millipore B-Bridge International, Inc. AAT Bioquest, Inc. Caliber Imaging and MO BIO Laboratories, Inc. BioChain Institute, Inc. LifeSensors, Inc. Diagnostics, Inc. Sarstedt, Inc. GE Healthcare MO BIO Laboratories, Inc. CoolLED Ltd. MO BIO Laboratories, Inc. NEOGEN CORPORATION Echo Laboratories Flow cytometer ScienCell Research Nacalai USA, Inc. ISS, Inc. Beckman Coulter Laboratories KEYENCE CORPORATION EMD Millipore UVP LLC Enzyme substrates Nikon Instruments, Inc. GE Healthcare AAT Bioquest, Inc. UVP LLC Miltenyi Biotec, Inc. Genomic DNAs LifeSensors, Inc. Sony Biotechnology, Inc. BioChain Institute, Inc. NEOGEN CORPORATION Fluorescence systems- Union Biometrica, Inc. GE Healthcare Nacalai USA, Inc. quantitative SGI-DNA, A Synthetic Vector Laboratories 3i Intelligent Imaging Genomics, Inc. Company Innovations ScienCell Research Laboratories
158 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting EXHIBITOR PRODUCT INDEX
Worthington Biochemical KEYENCE CORPORATION Image intensifiers BioChain Institute, Inc. Corporation Nanolive SA Hamamatsu Corporation Biosearch Technologies, Inc. ZenBio, Inc. Nikon Instruments, Inc. Olympus America Image processors Incubators Glassware Phase Focus Limited Echo Laboratories BINDER, Inc. World Precision Instruments, Photometrics KEYENCE CORPORATION Inc. QImaging Imaging - Ex vivo Okolab SRL ScienCell Research Caliber Imaging and UVP LLC Glycosidase inhibtors Laboratories Diagnostics, Inc. VitaScientific, Inc. EMD Millipore Sony Biotechnology, Inc. Echo Laboratories Reagency Pty Ltd. Union Biometrica, Inc. MatTek Corporation Incubators- refrigerated Phase Focus Limited BINDER, Inc. Gradient makers Image analysis software Accurate Chemical & 3i Intelligent Imaging Imaging-In vivo Information services Scientific Corporation Innovations 3i Intelligent Imaging Labroots, Inc. Bio-Rad Laboratories Innovations SBCF (French Society for Growth factors Bitplane, Inc. Caliber Imaging and Cell Biology) Abbiotec DRVision Technologies LLC Diagnostics, Inc. ScienCell Research Cell Signaling Technology GE Healthcare Echo Laboratories Laboratories EMD Millipore Hamamatsu Corporation MatTek Corporation Miltenyi Biotec, Inc. ibidi, LLC Miltenyi Biotec, Inc. Inhibitors ScienCell Research KEYENCE CORPORATION Olympus America EMD Millipore Laboratories Micro-Manager By Open Photometrics Funakoshi Company, Ltd. ZenBio, Inc. Imaging Sutter Instrument Company LifeSensors, Inc. Nanolive SA Nacalai USA, Inc. Nikon Instruments, Inc. Imaging-x-ray Reagency Pty Ltd. Olympus America Hamamatsu Corporation H Photometrics Injectors HPLC column QImaging Immunoassay system- Sutter Instrument Company EMD Millipore Sony Biotechnology, Inc. automated World Precision Instruments, Nacalai USA, Inc. UVP LLC NEOGEN CORPORATION Inc.
Hand protectors Image analyzer Immunochemicals Intensified cameras Funakoshi Company, Ltd. KEYENCE CORPORATION EMD Millipore Andor Technology MO BIO Laboratories, Inc. Motic Instruments Funakoshi Company, Ltd.
Phase Focus Limited Innova Biosciences Interference filters Homogenizers- tissue ScienCell Research Jackson ImmunoResearch Chroma Technology Corp MO BIO Laboratories, Inc. Laboratories Laboratories, Inc.
Sony Biotechnology, Inc. Reagency Pty Ltd. Ion-exchange resins Hot plates UVP LLC EMD Millipore Okolab SRL Immunohistochemistry World Precision Instruments, Image analyzer- FISH reagents/kits Isolators Inc. applications AAT Bioquest, Inc. World Precision Instruments,
KEYENCE CORPORATION Abcam, Inc. Inc. Hybridization incubator/ Nikon Instruments, Inc. Atlas Antibodies AB ovens Sony Biotechnology, Inc. B-Bridge International, Inc. Isotope-labeled UVP LLC BioChain Institute, Inc. compounds
Image analyzer- high Bioss, Inc. Atlas Antibodies AB Hybridization reagents resolution Cell Signaling Technology AAT Bioquest, Inc. ASI/Applied Scientific Cosmo Bio Co., Ltd BioChain Institute, Inc. Instrumentation Elabscience Biotechnology Biosearch Technologies, Inc. GE Healthcare Co.,Ltd J Vector Laboratories Job fairs KEYENCE CORPORATION Funakoshi Company, Ltd. AAAS Nikon Instruments, Inc. Innova Biosciences Hybridomas Labroots, Inc. Sony Biotechnology, Inc. Jackson ImmunoResearch FiberCell Systems, Inc. SBCF (French Society for Laboratories, Inc. Cell Biology) Image analyzer- high LifeSensors, Inc.
speed Nacalai USA, Inc. I ASI/Applied Scientific Vector Laboratories Image analysis Instrumentation VitaScientific, Inc. 3i Intelligent Imaging KEYENCE CORPORATION K Innovations Sony BiotechnologyInc. Immunological substances- Kinases/phosphatases Allele Biotechnology natural/human B-Bridge International, Inc. Bio-Rad Laboratories Image analyzer- ratiometric Funakoshi Company, Ltd. Cell Signaling Technology Bitplane, Inc. dyes Nacalai USA, Inc. Cosmo Bio Co., Ltd. DRVision Technologies LLC Nikon Instruments, Inc. ZenBio, Inc. Nacalai USA, Inc. Etaluma, Inc. Reagency Pty Ltd. GE Healthcare In situ Worthington Biochemical Allele Biotechnology Corporation
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 159 EXHIBITOR PRODUCT INDEX
L Liquid handling- Membrane filters Microscopes automated EMD Millipore 3i Intelligent Imaging Laboratory apparatus- Beckman Coulter VitaScientific, Inc. Innovations miscellaneous TECAN ASI/Applied Scientific Electron Microscopy Takasago Fluidic Systems Micro- incubators Instrumentation Sciences Okolab SRL Bruker Corporation MO BIO Laboratories, Inc. Low-level counting Caliber Imaging and NanoAnalytics GmbH systems Microcarriers Diagnostics, Inc. PicoQuant Photonics North ISS, Inc. Union Biometrica, Inc. Echo Laboratories America, Inc. Etaluma, Inc. SmarAct GmbH Luminescence imaging Microdissecting KEYENCE CORPORATION VitaScientific, Inc. Echo Laboratories instruments Leica Microsystems, Inc.
Olympus America World Precision Instruments, Logos Biosystems, Inc. Laboratory furniture VitaScientific, Inc. Inc. Mightex Systems VitaScientific, Inc. Motic Instruments
Luminometer Microforges Nanolive SA Laboratory workstation- TECAN World Precision Instruments, Nikon Instruments, Inc. automated Inc. Olympus America Beckman Coulter Phase Focus Limited GE Healthcare Microinjector Sony Biotechnology, Inc.
M ASI/Applied Scientific Sutter Instrument Company Laminar flow equipment Magnetic particles Instrumentation TESCAN USA, INC. ibidi, LLC Accurate Chemical & Sutter Instrument Company VitaScientific, Inc. Scientific Corporation World Precision Instruments, World Precision Instruments, Laser scanning BioChain Institute, Inc. Inc. Inc. ISS, Inc. EMD Millipore
Nikon Instruments, Inc. MO BIO Laboratories, Inc. Micromanipulators Microscopes- atomic Olympus America Nacalai USA, Inc. ASI/Applied Scientific force
Instrumentation JPK Instruments AG Lasers Magnetic particles Nikon Instruments, Inc. 3i Intelligent Imaging processor SmarAct GmbH Microscopes- automated Innovations MO BIO Laboratories, Inc. World Precision Instruments, scanning Nikon Instruments, Inc. Inc. 3i Intelligent Imaging PicoQuant Photonics North Magnetic particles Innovations America, Inc. separator Microplate fluorometer ASI/Applied Scientific EMD Millipore BMG Labtech Instrumentation Light boxes MO BIO Laboratories, Inc. TECAN Etaluma, Inc. UVP LLC Nacalai USA, Inc. GE Healthcare VitaScientific, Inc. Microplate readers KEYENCE CORPORATION Magnetic stirrers BMG Labtech Leica Microsystems,Inc. Light sources VitaScientific, Inc. TECAN Nikon Instruments, Inc. Chroma Technology Corp World Precision Instruments, Sutter Instrument Company CoolLED Ltd. Inc. Microplate washers TESCAN USA, INC. Hamamatsu Corporation TECAN ISS, Inc. Mass spectral analysis Microscopes- confocal Mightex Systems Cell Signaling Technology Micropositioners 3i Intelligent Imaging Nikon Instruments, Inc. Funakoshi Company, Ltd. ASI/Applied Scientific Innovations Sutter Instrument Company Instrumentation Andor Technology UVP LLC Mass spectrometers SmarAct GmbH Bruker Corporation World Precision Instruments, Lipotype GmbH World Precision Instruments, Caliber Imaging and Inc. Inc. Diagnostics, Inc. Meetings GE Healthcare Lipids AAAS Microscope slides ISS, Inc. Accurate Chemical & Abcam, Inc. ibidi, LLC KEYENCE CORPORATION Scientific Corporation Institut Curie MatTek Corporation Leica Microsystems, Inc. Funakoshi Company, Ltd. Keystone Symposia Nikon Instruments, Inc. Lipotype GmbH Labroots, Inc. Microscope slides- Olympus America SBCF (French Society for printed TESCAN USA, INC. Lipids- concentrated Cell Biology) ibidi, LLC Lipotype GmbH ScienCell Research PhalanxBio, Inc. Microscopes- motorized Laboratories stages Liposome Microscope slides- 3i Intelligent Imaging Lipotype GmbH Membership specialty Innovations AAAS EMD Millipore ASI/Applied Scientific Liquid dispensers Keystone Symposia ibidi, LLC Instrumentation TECAN Labroots, Inc. Nanolive SA Etaluma, Inc. SBCF (French Society for PhalanxBio, Inc. KEYENCE CORPORATION Cell Biology) Leica Microsystems, Inc.
160 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting EXHIBITOR PRODUCT INDEX
Nikon Instruments, Inc. SGI-DNA, A Synthetic PCR purification kit SmarAct GmbH Genomics, Inc. Company B-Bridge International, Inc. Sony Biotechnology, Inc. Vector Laboratories O BioChain Institute, Inc. Oligonucleotide modification Sutter Instrument Company Worthington Biochemical MO BIO Laboratories, Inc. reagents Corporation ScienCell Research AAT Bioquest, Inc. Microscopes- optical & Laboratories Innova Biosciences accessories Molecular biology
3i Intelligent Imaging software PCR reagents Oligonucleotide probes- Innovations SGI-DNA, A Synthetic AAT Bioquest, Inc. custom labeled ASI/Applied Scientific Genomics, Inc. Company B-Bridge International, Inc. AAT Bioquest, Inc. Instrumentation Bio-Rad Laboratories Biosearch Technologies, Inc. CoolLED Ltd. Monochromators BioChain Institute, Inc.
Echo Laboratories ISS, Inc. Funakoshi Company, Ltd. Oligonucleotide Etaluma, Inc. TECAN GeneCopoeia, Inc. synthesizer Leica Microsystems, Inc. MO BIO Laboratories, Inc. SGI-DNA, A Synthetic Logos Biosystems, Inc. Monoclonal antibodies Nacalai USA, Inc. Genomics, Inc. Company Nikon Instruments, Inc. Abbiotec New England Biolabs
Okolab SRL Abcam, Inc. ScienCell Research Oligonucleotides Sutter Instrument Company Accurate Chemical & Laboratories AAT Bioquest, Inc. Scientific Corporation Biosearch Technologies, Inc. Microscopes- scanning Allele Biotechnology Particle counters PhalanxBio, Inc. electron Cell Signaling Technology Beckman Coulter SGI-DNA, A Synthetic Nikon Instruments, Inc. EMD Millipore Genomics, Inc. Company TESCAN USA, INC. Elabscience Biotechnology Particle size analyzers
Co., Ltd. Beckman Coulter Online services Microscopes- scanning LifeSensors, Inc. KEYENCE CORPORATION Labroots, Inc. probe Pacific Immunology Union Biometrica, Inc. ScienCell Research Bruker Corporation Reagency Pty Ltd. Laboratories JPK Instruments AG VitaScientific, Inc. Peptides- pharmaceutical
grade Optical filters Microscopes- scanning Monoclonal antibodies- large VitaScientific, Inc. Chroma Technology Corp tunnelling scale production Nikon Instruments, Inc. Bruker Corporation Abbiotec Peptides- synthetic Olympus America Cell Signaling Technology Abbiotec Sutter Instrument Company Microscopes- stereo LifeSensors, Inc. World Precision Instruments, Leica Microsystems, Inc. Inc. Motic Instruments Perfusion system
Nikon Instruments, Inc. N EMD Millipore NMR spectroscopy Optical interference Olympus America Takasago Fluidic Systems Innova Biosciences filters World Precision Instruments, Chroma Technology Corp Inc. Peroxidase conjugated Neurochemicals Nikon Instruments, Inc. antibody Abcam, Inc. Olympus America Microscopy accessories- Cell Signaling Technology Bio-Techne Sutter Instrument Company fluorescence filters Innova Biosciences Reagency Pty Ltd. 3i Intelligent Imaging Jackson ImmunoResearch Organic laboratory Innovations Laboratories, Inc. Nitric oxide reagents chemicals Chroma Technology Corp Vector Laboratories AAT Bioquest, Inc. EMD Millipore CoolLED Ltd.
Echo Laboratories Petri dishes Nucleic acid blotting Ovens Logos Biosystems, Inc. ibidi, LLC EMD Millipore BINDER, Inc. Motic Instruments MatTek Corporation Vector Laboratories VitaScientific Inc. Nikon Instruments, Inc. Sarstedt, Inc.
Olympus America VitaScientific, Inc. Nucleic acid isolations Oxygen meters Photometrics B-Bridge International, Inc. Okolab SRL QImaging Pharmaceutical development BioChain Institute, Inc. Sutter Instrument Company equipment MO BIO Laboratories, Inc. Takasago Fluidic Systems PhalanxBio, Inc. Mixers UVP LLC P Sarstedt, Inc. PCR kits Phenols
AAT Bioquest, Inc. EMD Millipore Nucleic acid synthesizer Molecular biology B-Bridge International, Inc. SGI-DNA, A Synthetic reagents BioChain Institute, Inc. Photographic equipment- Genomics, Inc. Company AAT Bioquest, Inc. Funakoshi Company, Ltd. time lapse moving
B-Bridge International, Inc. ScienCell Research picture Nucleotides Biosearch Technologies, Inc. Laboratories KEYENCE CORPORATION AAT Bioquest, Inc. Cellecta, Inc. UVP LLC EMD Millipore GeneCopoeia, Inc. MO BIO Laboratories, Inc.
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 161 EXHIBITOR PRODUCT INDEX
Photometers Protein purification Radioimmunoassay St. Jude Children’s Research TECAN system service Hospital Accurate Chemical & ZenBio, Inc. Photomicrographic Scientific Corporation Restriction enzymes equipment Bio-Rad Laboratories Reagent grade water New England Biolabs Etaluma, Inc. BioChain Institute, Inc. system Nikon Instruments, Inc. EMD Millipore ScienCell Research Robotic slide handler LifeSensors, Inc. Laboratories Nikon Instruments, Inc. Phycoexythrim conjugated Nacalai USA, Inc. antibodies Reagent- dog Robotics Jackson ImmunoResearch Pumps Cell Signaling Technology SmarAct GmbH Laboratories, Inc. Takasago Fluidic Systems ScienCell Research TECAN World Precision Instruments, Laboratories Pipette pullers Inc. Sony Biotechnology, Inc. Sutter Instrument Company World Precision Instruments, Pumps- peristaltic Reagent- rat S Safety equipment Inc. Takasago Fluidic Systems Bio-Rad Laboratories MO BIO Laboratories, Inc. World Precision Instruments, Cell Signaling Technology
Pipettes Inc. ScienCell Research Sample preparation- Sarstedt, Inc. Laboratories equipment Sutter Instrument Company Pumps- vacuum Sony Biotechnology, Inc. TECAN VitaScientific, Inc. World Precision Instruments,
Inc. Reagents Scientific information Pipettes- automatic AAT Bioquest, Inc. services Fluicell AB Purification of Abcam, Inc. Keystone Symposia antibodies Bio-Rad Laboratories Labroots, Inc. Pipettes- micro Abbiotec Bio-Techne SBCF (French Society for Sutter Instrument Company Elabscience Biotechnology Cell Signaling Technology Cell Biology) World Precision Instruments, Co., Ltd. Cytoskeleton, Inc. SGI-DNA, A Synthetic Inc. Innova Biosciences EMD Millipore Genomics, Inc. Company Nacalai USA, Inc. Funakoshi Company, Ltd. Scientific Volume Imaging Plasma Innova Biosciences
ZenBio, Inc. Purified proteins Jackson ImmunoResearch Scientific software Abbiotec Laboratories, Inc. 3i Intelligent Imaging Plastic laboratory ware Atlas Antibodies AB Miltenyi Biotec, Inc. Innovations Sarstedt, Inc. B-Bridge International, Inc. NEOGEN CORPORATION Echo Laboratories VitaScientific, Inc. Cosmo Bio Co., Ltd Nacalai USA, Inc. Micro-Manager By Open Cytoskeleton, Inc. Polyplus-transfection Imaging Professional societies EMD Millipore Reagency Pty Ltd. Scientific Volume Imaging AAAS LifeSensors, Inc. Seahorse Bioscience, Inc. Sutter Instrument Company Keystone Symposia Nacalai USA, Inc. ScienCell Research
VitaCyte LLC Laboratories Serum substitutes Protein binding studies Worthington Biochemical Sony Biotechnology, Inc. ScienCell Research Nacalai USA, Inc. Corporation Vector Laboratories Laboratories VitaScientific, Inc. ZenBio, Inc. Protein blotting ZenBio, Inc.
Cell Signaling Technology Serum-free media EMD Millipore Q Reagents- mouse Quantitation kits Funakoshi Company, Ltd. Nacalai USA, Inc. Bio-Rad Laboratories Cytoskeleton, Inc. ScienCell Research Vector Laboratories Cell Signaling Technology Elabscience Biotechnology Laboratories ScienCell Research Co., Ltd. ZenBio, Inc. Protein expression Laboratories Funakoshi Company, Ltd. Allele Biotechnology Sony Biotechnology, Inc. ScienCell Research Serums Cell Signaling Technology Laboratories EMD Millipore Cosmo Bio Co., Ltd Recruiting Jackson ImmunoResearch Cytoskeleton, Inc. Institut Curie Laboratories, Inc. Elabscience Biotechnology ScienCell Research Co.,Ltd Refrigerated centrifuges R Laboratories FiberCell Systems, Inc. RNA isolation VitaScientific, Inc. Vector Laboratories LifeSensors, Inc. Allele Biotechnology ZenBio, Inc. Mirus Bio LLC B-Bridge International, Inc. Refrigerators
New England Biolabs BioChain Institute, Inc. VitaScientific, Inc. Shakers MO BIO Laboratories, Inc. MO BIO Laboratories, Inc. Protein kinase assay kit Nacalai USA, Inc. Research awards- Sarstedt, Inc. Cell Signaling Technology PhalanxBio, Inc. postdoctoral VitaScientific, Inc. ScienCell Research Keystone Symposia
Laboratories SBCF (French Society for UVP LLC Cell Biology)
162 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting EXHIBITOR PRODUCT INDEX
Signal transduction PhalanxBio, Inc. Tissue cultures Vibration isolation reagents ScienCell Research Accurate Chemical & equipment Montana Molecular Laboratories Scientific Corporation World Precision Instruments, Scientific Volume Imaging Allele Biotechnology Inc. Software ZenBio, Inc. B-Bridge International, Inc. DRVision Technologies LLC MatTek Corporation Video microscopy Micro-Manager By Open Television microscope Mirus Bio LLC systems Imaging systems Polyplus-transfection Caliber Imaging and Nikon Instruments, Inc. Echo Laboratories ZenBio, Inc. Diagnostics, Inc. Scientific Volume Imaging KEYENCE CORPORATION Echo Laboratories Tissue dissociation KEYENCE CORPORATION Solvents for HPLC Temperature controllers enzymes Nikon Instruments, Inc. EMD Millipore Okolab SRL Nacalai USA, Inc. Sony Biotechnology, Inc. World Precision Instruments, VitaCyte LLC VitaScientific, Inc. Spectral bio-imaging Inc. Worthington Biochemical Nikon Instruments, Inc. Corporation Viral reagents Sutter Instrument Company Temperature probes Allele Biotechnology World Precision Instruments, Tissue processors Cellecta, Inc. Spectrofluorometers Inc. Miltenyi Biotec, Inc. ISS, Inc. Viruses TECAN Test tube racks Transfection kits Allele Biotechnology Sarstedt, Inc. ibidi, LLC Spectrometers Mirus Bio LLC Andor Technology Thermal cycler ScienCell Research Mightex Systems Bio-Rad Laboratories Laboratories W Water bath shakers NanoAnalytics GmbH UVP LLC VitaScientific, Inc. VitaScientific, Inc. TECAN
World Precision Instruments, Tissue culture CO2 Tube rotators Water baths Inc. incubator Miltenyi Biotec, Inc. VitaScientific, Inc. ibidi, LLC
Spectrophotometer cells KEYENCE CORPORATION Tubings Western blotting World Precision Instruments, Okolab SRL Accurate Chemical & equipment Inc. Scientific Corporation Bio-Rad Laboratories Tissue culture apparatus Cytoskeleton, Inc. Spectrophotometer light FiberCell Systems, Inc. Tyrosine kinase EMD Millipore sources ibidi, LLC inhibitors GE Healthcare Hamamatsu Corporation KEYENCE CORPORATION Cell Signaling Technology UVP LLC World Precision Instruments, MatTek Corporation Reagency Pty Ltd. Inc. NanoAnalytics GmbH Takasago Fluidic Systems Spectrophotometer- micro Tissue culture inserts U UV fluorescence diagnostic Hamamatsu Corporation ibidi, LLC equipment NanoAnalytics GmbH Echo Laboratories Spectrophotometers
EMD Millipore Tissue culture labware Ultracentrifuge TECAN Applied BioPhysics, Inc. Beckman Coulter FiberCell Systems, Inc.
Spin columns ibidi, LLC Ultrasonic cleaners EMD Millipore MatTek Corporation World Precision Instruments, MO BIO Laboratories, Inc. Nacalai USA, Inc. Inc. VitaScientific, Inc. Sarstedt, Inc. VitaScientific, Inc. Stimulators World Precision Instruments, Tissue culture media V Inc. B-Bridge International, Inc. Vacuum system FiberCell Systems, Inc. accessories Surgical instruments Funakoshi Company, Ltd. SmarAct GmbH Fine Science Tools MatTek Corporation World Precision Instruments, Mirus Bio LLC Valves Inc. Nacalai USA, Inc. Takasago Fluidic Systems ZenBio, Inc. Vectors T Tissue culture sera- human Cellecta, Inc. based EMD Millipore Technical consulting ZenBio, Inc. 3i Intelligent Imaging
Innovations Lipotype GmbH
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 163 EXHIBITOR PRODUCT INDEX Notes
164 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting ABSTRACT AUTHOR INDEX ABSTRACT AUTHOR INDEX Oral Presentations The names listed below are authors of abstract-based oral presentations. Poster presentation authors are listed separately in the Poster Guide. The numbers below refer to the presentation numbers listed in this Program. E=Microsymposium Talk; M=Minisymposium Talk; S=Symposium Talk; A=Award Talk (including Kaluza Minisymposia); G=All Other Talks
Baserga, Susan J...... M30 Brugués, Agustí ...... M162 A Bathe, Mark ...... E28,E36 Brunet, Anne ...... M227 Abdullah, Christopher ...... M55 Battaglia, Giuseppe ...... M127 Bryant, Kirsten ...... M87 Adam, Kuspa ...... E54 Bauer, Hilary ...... M197 Bryant, Zev ...... M42 Adam, Stephen A...... E75,M95 Baumann, Matthias ...... M79 Bryce, Nicole S...... E95 Adelstein, Robert S...... M157 Baumeister, Wolfgang ...... M147 Buchwalter, Abigail L...... E74 Adler, Jacob J...... M56 Bausch, Andreas R...... M44 Buck, Kenneth B...... M154 Aguet, Francois ...... E15,M180 Bays, Jennifer L...... E120 Budin, Itay ...... M74 Ahmed, Ijaz ...... M177 Bazellierres, Elsa ...... M162 Budnar, Srikanth ...... M164 Aitchison, John D...... E62 Bearer, Elaine L...... M170 Bun, Philippe ...... M156 Albert, Sahradha ...... M147 Becalska, Agata N...... M175 Burdyniuk, Mariia ...... E66 Alberti, Simon ...... M102,M148 Beck, Moriah R...... E94 Bureau, Cecile ...... E91 Albiges-Rizo, Corinne ...... M165 Beckham, Yvonne ...... E34 Burgess, Jason ...... M76 Aleman, Lourdes M...... M59 Belcher, Angela ...... S12 Burke, Thomas A...... M199 Alieva, Naila O...... E125 Belmont, Andrew S...... M31 Busch, David J...... M38 Almassalha, Luay ...... M95 Belmont, Lisa D...... M111 Bush, Jeffrey O...... M228 Alper, Joshua ...... M86 Bement, William M...... M121 Byrne, Alexandra B...... M171 Alushin, Gregory M...... E3 Ben-David, Uri ...... A5 Byron, Adam ...... E92 Anastasiadis, Panos Z...... E85 Bergman, Jared ...... E45 Anderson, Trevor ...... M54 Bergson, Clare ...... E58 Angel, Stephanie ...... E99 Berman, Judith ...... A4 Anselmi, Claudio ...... M33 Bershadsky, Alexander D...... E124,E125 C Cabral, Gabriela ...... M109 Anseth, Kristi ...... S13 Betzig, Eric ...... E15,M169, Calderon, Abram ...... M184 Anthony, Sarah R...... E14 M180,M47,M68,S1 Calizo, RhodoraCristina �������������������������������������E41 Antonescu, Costin N...... M73 Bewersdorf, Joerg ...... E21,M50 Camacho-Perez, Marcial ����������������������������������� M34 Ao, Mingfang ...... E1 Bezanilla, Magdalena ...... E97 Campbell, Sharon L...... A11 Appukutty, Abhinav ...... M49 Biggs, Ronald J...... M8 Canman, Julie C...... E24 Arakel, Eric C...... M213 Biris, Octavian ...... M170 Carr, Jennifer M...... E85 Aravamudhan, Pavithra ...... A6,E115 Biro, Maté ...... M155,M160 Carrasco, Silvia ...... M191 Archambault, Vincent ...... M183 Black, Ben E...... M184,M21 Case, Lindsay B...... A11 Arellano-Santoyo, Hugo ...... M82 Blackstone, Craig D...... M169 Caspari, Oliver D...... M101 Arnaoutov, Alexei ...... M18 Blainey, Paul ...... E36 Cassani, Davide ...... M158 Arndt, Greg M...... E95 Blanchoin, Laurent ...... M46 Castracane, James ...... E4 Arnold, Torey R...... M125 Blehm, Benjamin H...... M140 Castro, Natalia ...... E121 Arpağ, Göker ...... M43 Bloom, Kerry S...... E117 Ceraj, Ivan ...... M59 Arumugam, Senthil ...... E15 Bloomekatz, Joshua ...... M4 Cervero, Pasquale ...... E7 Asan-Liski, Hilal ...... E93 Blum, Thorsten ...... M33 Cetera, Maureen ...... M226 Asano, Shoh ...... M147 Boden, Annett ...... M155 Cha, Diana J...... E42 Asbury, Charles L...... M45 Bodin, Stephane ...... M162 Chait, Brian T...... M28 Ast, Tslil ...... M213 Boggetti, Barbara ...... M222 Chandhok, Mana ...... M6 Ataullakhanov, Fazly I...... M184 Boitano, Scott ...... E9 Chanet, Soline ...... M137 Athirasala, Avathamsa ...... E108 Boke, Elvan ...... M148 Chang, Yi-Ren ...... E39 Audhya, Anjon ...... M37 Bokil, Nilesh J...... M93 Chao, Jesse T...... M182 Auer, Manfred ...... M3 Bone, Leslie N...... M73 Chapman, Edwin R...... E29 Augenlicht, Alice ...... E92 Bonne, Gisele ...... E70 Charest, Pascale G...... M13 Auld, Alexander L...... E71 Bonventre, Joseph V...... E55 Charras, Guillaume ...... M155 AustinII, Jotham ...... M205 Boothby, Thomas C...... M115 Chatzifrangkeskou, Maria �����������������������������������E70 Aviram, Naama ...... M213 Borek, Bartlomiej ...... E67 Chau, Deming ...... M176 Awe, Adam M...... E29 Botelho, Roberto J...... M73 Chavali, Arvind K...... M190 Axelrod, Jeffrey D...... M229 Bouiossou, Anais ...... E7 Chaves, Frances ...... M170 Ayres, Virginia M...... M177 Bovellan, Miia ...... M155 Che, Daphne ...... M152 Boyden, Ed ...... E36 Cheerambathur, Dhanya K...... M19,M81 Boyer, Thomas G...... E79 Cheeseman, Iain M...... E28,M63 Bradford, Dan ...... A4 Chen, Baohui ...... M130 B Bradshaw, Niels ...... M12 Baba, Satoshi ...... M203 Chen, Bi-Chang ...... M180,M47 Brady, Owen A...... M92 Bachir, Alexia ...... E123 Chen, Guangbo ...... A4 Brand, Cristoph A...... M166 Backman, Vadim ...... M95 Chen, Jieyan V...... M61 Brangwynne, Clifford P...... E40,M149 Bahmanyar, Shirin ...... E21 Chen, Renjie ...... E115 Braun, Erez ...... M141 Bahrami-Nejad, Zahra ...... M188 Chen, Simeng ...... M62 Brauneis, Alison L...... M59 Baird, Michelle A...... M157,A11 Chen, Vivian K...... M101 BravoCordero, JoseJavier ...... E56,M163 Bajar, Bryce ...... M191 Chen, Wei-Sheng ...... E13 Breckels, Lisa M...... M144 Baker, David ...... E6 Chen, Yalei ...... E46 Brewer, Bryson M...... E1 Balla, Tamas ...... M72 Chen, Yu ...... M31 Brian, Galletta J...... M60 Balland, Martial ...... M165 Chen, Yunfeng ...... E121 Brieher, William M...... M153 Ballister, Edward R...... M184 Cheng, Richard ...... M231 Brill, Julie A...... M76 Balogi, Zsolt ...... M133 Cheng, Xiutang ...... M216 Brinkman, Eva ...... M31 Banigan, Edward ...... M95 Cheng-Zhong, Zhang ...... M27 Brouwers, Nathalie ...... M204 Baranger, Anne ...... M57 Chew, SingYian Y...... M159 Brown, Alex H...... M72 Barlan, Kari ...... M226 Chi, Qiuyi ...... E16 Brown, James B...... E65 Barnhart, Meghan C...... M24 Chiaruttini, Nicolas ...... M122 Brownell, Sara E...... M51 Barry, Zachary ...... E28 Chien, Yuan-Hung ...... E90 Broyer, Risa M...... M116 Bartles, James R...... M161 Chiew, Geraldine Giap Ying �������������������������������E52 Brozena, Alexandra H...... M115
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 165 ABSTRACT AUTHOR INDEX
Chmatal, Lukas ...... A7,M21 Denker, Annette ...... M219 Choi, Soo H...... E100 Dent, Erik W...... E29 F Chook, YuhMin ...... E109 Der, Channing ...... M87 Fachinetti, Daniele ...... M22 Chou, Chia-Fu ...... E39 DeRisi, Joe ...... M14 Fagerstrom, Carey J...... M60 Chowdary, Praveen ...... M39 Derivery, Emmanuel ...... M129 Failes, Tim ...... E95 Christensen, Jenna R...... M161 Derksen, Patrick W...... M124 Faklaris, Orestis ...... E38 Christoforou, Andy ...... M144 Dernburg, Abby F...... M29 Falk, Samantha J...... M21 Chuartzman, Silvia G...... M213 Derrer, Carina ...... M97 Falzone, Tomas ...... M170 Chugh, Priyamvada ...... M119, Desai, Arshad B...... E111,M19, Fan, Chen-Ming ...... M7 M155,M158 M8,M81 Fang, Mark ...... M99 Chung, Jeeyun ...... M71 DeSantis, Morgan ...... M78 Faraldo-Gomez, Jose ...... M33 Churchman, Stirling ...... M218 Descroix, Stéphanie ...... E91 Feeser, Elizabeth ...... M43 Cimini, Daniela ...... M17 Destaing, Olivier ...... M165 Feldman, Jessica L...... M108 Clark, Alison D...... E79 Deutscher, Roswell C...... M75 Felt, Kristen D...... E119 Cleveland, Don W...... E114,M22 DeVico, Anthony L...... M173 Feric, Marina ...... M149 Coccuci, Emmanuel ...... M180 Devine, Alexus ...... M140 Fernandez-Gonzalez, Rodrigo ���������������������������E50 Coffey, Robert J...... E42 Devos, Damien ...... M207 Ferraro, Davide ...... E91 Cogswell, Carol J...... M62 Di, Yingtong ...... M131 Fessenden, Tim ...... E34 Collinet, Claudio ...... M136 Diab, Heba I...... M92 Fetters, Halie ...... M118 Collins, Mary A...... E72 Didovyk, Andriy ...... E67 Firtel, Richard A...... M198 Comunale, Franck ...... M162 Dillard, Pierre ...... E122 Fischer, Robert S...... M157 Condeelis, John S...... E35,E4, Ding, Wandi ...... M189 Fletcher, Daniel A...... M193 E56,E64,M163 Ding, Xiao ...... M131 Folker, Eric S...... E71,E72 Conkright, Juliana ...... A4 Dinshaw, Kayla M...... M125 Foltz, D.Daniel ...... M24 Conte, Vito ...... M225 Disatnik, Marie-Helene ...... E11 Fontoura, Beatriz M...... M98 Coombes, Courtney E...... M86 Discher, Dennis E...... E108 Fornasiero, Eugenio F...... M219 Cooper, Robert M...... M174 Dmitrieff, Serge ...... M156 Forsburg, Susan L...... M26 Corbett, Kevin D...... M29 Dodd, Rebecca J...... E12 Forscher, Paul ...... M154 Cormack, Robert H...... M62 Dolat, Lee ...... E103 Forth, Scott ...... M68 Cornejo, Elias ...... M212,S16 Dominguez, Roberto ...... M199 Foster, Peter J...... E43 Cornils, Hauke ...... M27 Dong, Zigang ...... M25 Francis, Joshua M...... M27 Costa, Elizabeth A...... M213 Donnelly, Sara K...... M163 Franco, Omar E...... E1 Coutinho, Kester ...... M3 Doudna, Jennifer A...... S11, M99 Franzmann, Titus M...... M102,M220 Couvillion, Mary ...... M218 Douglas, Temple A...... M17 Freedman, Benjamin S...... E55 Covert, Markus W...... M191 Draper, Bruce W...... S17 Freeman, Scott ...... M51 Cowdrey, Diana A...... E29 Drechsel, David N...... M148 FreemanRosenzweig, Elizabeth S. ����������������� M101 Cox, Dianne ...... E64,M58 Drechsler, Maik ...... E49 Friedman, Jonathan R...... M105 Creamer, Matthew S...... M154 Drexler, Hannes C...... M79 Fritz-Laylin, Lillian ...... M196 Cross, Frederick R...... M200 Driver, Jonathan W...... M45 Fritzsche, Marco ...... M155 Crowe, Alison ...... M51,M56 Drubin, David G...... M39 Fu, Afu ...... E52 Cruz, Anthony ...... E20 Duan, Liting ...... M152 Fuchs, Elaine ...... A12,E114,M230 Cui, Bianxiao ...... E10,M152,M39 Dudin, Omaya ...... M15 Fürthauer, Sebastian ...... E43 Cui, Yi ...... M39 Dudley, Andrew C...... E78 Fusetti, Fabrizia ...... M198 Cyert, Martha S...... M90 Dufresne, Eric R...... M154 Czapla, Heather ...... M71 Dultz, Elisa M...... M100 Dumesic, Phillip A...... A8 Dumont, Julien ...... E24 G Gaillard, Jeremie ...... M46 Dumont, Sophie ...... E116 Gallego, Oriol ...... M207 D Dunn, Ariel ...... M4 Daeden, Alicia ...... M129 Galperin, Emilia ...... E8 Dutton, Rachel J...... S7 Damle, Nikhil ...... M90 Gandhi, Saumil ...... E56 Dye, Natalie A...... M224 Dammermann, Alexander �������������������������������� M109 Ganguly, Sujoy ...... E49 Dang, Suparna ...... E53 Gao, Xing ...... S17 Dange, Thomas ...... M97 GarcíaAznar, José M...... M225 Danglot, Lydia A...... E38 E Garcia-Sastre, Adolfo ...... M98 Danuser, Gaudenz ...... E123, Eaton, Suzanne ...... M224 Garde, Ryan J...... E86 M168,M180 Eddy, Robert J...... E35,E4,E64 Gardel, Margaret L...... E34,M166 daSilvaFerreira, Fernando ����������������������������������S17 Edes, Kornelia ...... M179 Gardner, Melissa ...... M86 Dasso, Mary ...... M18 Edwardson, J.Michael ...... M37 Gasic, Ivana ...... M67 Daum, Bertram ...... M33 Efremov, Artem K...... E125 Gatto, Laurent ...... M144 Davey, Norman E...... M90 Egelhofer, Thea ...... A9 Gaudet, Suzanne ...... M190 Davidson, Lance A...... E89,M137 Eggert, Ulrike S...... E23 Gaudin, Raphael ...... E15,M180,M36 Davidson, Michael W...... A11 Eichel, Kelsie ...... E104 Gautam, N ...... M88 Davies, Karen M...... M33 Elbaum-Garfinkle, Shana �����������������������������������E40 Gauthier, Nils C...... M159 Davies, Tim ...... E24 Elosegui-Artola, Alberto ...... E121 Gauthier-Rouviere, Cecile ������������������������������� M162 Davis, Allyson ...... M118 Emery, Gregory ...... M183 Gavrilman, Anna ...... M59 Davoudi, Sadegh ...... M231 Emr, Scott ...... M133 Gaydos, Laura J...... A9 Day, Charles ...... M25 Engel, Benjamin D...... M147 Geffken, Kurt ...... E77 Day, Kasey J...... M205 Erdogan, Begum ...... E1 Geimer, Stefan ...... M101 Dayam, Roya M...... M73 Erdument-Bromage, Hediye �������������������������������E82 Geladaki, Aikaterini ...... M144 Debnath, Jayanta ...... E105 Erickson, Brian K...... M151 Gelfand, Vladimir I...... M84 DeCamilli, Pietro ...... M71 Erickson, Harold P...... M202 Gerlich, Daniel W...... M122 DeFea, Kathryn A...... E9 Erickson, Jared R...... M197 Gerson, Adina ...... M8 deGroot, Jolien ...... M124 Ermentrout, Bard ...... M137 Gerson-Gurwitz, Adina ...... E111 Deguchi, Takahiro ...... E92 Escribano, Jorge ...... M225 Gerst, Jeffrey E...... E106 deJong, Danielle ...... M124 Eskin, Julian ...... E96 Gertsik, Natalya ...... M176 Dekker, Cees ...... E22 Eskin, Tania L...... M40 Geyer, Elisabeth ...... M45 DeLaurentiis, Annamaria ������������������������������������E95 Ettinger, Andreas W...... E48 Ghiretti, Amy E...... E44 DelBel, Lauren M...... M76 Ewald, Andrew J...... M3 Ghosh, Abhishek ...... E88 delCastillo, Urko ...... M84 Gilbert, Penney M...... M231 Deline, Marshall ...... E21 Gillette, Jennifer M...... E12 DeMali, Kris A...... E120 Girard, Juliet R...... M178 Deng, Zhaoxuan ...... M66 Denic, Vlad ...... M110
166 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting ABSTRACT AUTHOR INDEX
Gittzus, Jordan ...... M175 Harvey, Kirsten ...... M169 Islam, AFM T...... M13 Glick, Benjamin S...... M205 Haßdenteufel, Sarah ...... M213 Itakura, Alan ...... M101 Glieder, Anton ...... E100 Hassen, Wafa ...... E69 Ito, Ami ...... M64 Glotzer, Michael ...... M120,M166 Hasty, Jeff ...... E67,M174 Ito, Hidenori ...... E51 Go, AllysaMarie M...... E118 Haswell, Elizabeth S...... M201 Ivanova, Pavlina T...... M72 Godzi, Maxim ...... M184 Hatch, Anna ...... M107 Ivanovska, Irena L...... E108 Gokce, Ozgun ...... E81 Haug, Jeffrey ...... A4 Ivarsson, Ylva ...... M90 Goldfarb, Alan A...... A6 Hauser, Meghan ...... M146 Iwamoto, Ikuko ...... E51 Goldman, Anne E...... E75 Hayden, Carl C...... M38 Iwata-Otsubo, Aiko ...... M21 Goldman, Robert D...... E75,M95 Hayes, James B...... M189 Iyengar, Ravi ...... E41 Goldstein, Bob ...... M115 Hayward, Simon W...... E1 Iyer, Jyoti ...... E26 Gomez, Guillermo A...... M164 He, Kangmin ...... M180,M36 Gonzalez-Gaitan, Marcos �������������������������������� M129 Heald, Rebecca ...... M23,S14 Goode, Bruce L...... E96 Heemskerk, Idse ...... M143 Goodenough, Ursula ...... M101 Heidema, Christy ...... E120 J Jackson, Emily K...... M27 Gorbsky, Gary J...... M20 Heisenberg, Carl-Philipp ...... M135 Jackson, Timothy R...... E89 Gordon, Deborah M...... S5 Henne, Mike ...... M133 Jacobs, Russell E...... M170 Gordonov, Simon ...... E36 Hennen, Jared ...... E76 Jacobs-Wagner, Christine ������������������������������� M150 Gorfe, Alex ...... M77 Hennig, Katharina ...... M165 Jacquemet, Guillaume ...... E92 Gorur, Amita ...... M203 Henty-Ridilla, Jessica L. �������������������������������������E96 Jahed, Zeinab ...... E98 Goryachev, Andrew ...... M121 Herranz-Pérez, Vicente ...... E101 Jalilian, Iman ...... E31 Goshima, Gohta ...... M64 Hershey, David ...... S16 Jan, Calvin H...... M213 Gough, Albert H...... M112 Hertz, Nicholas T...... E61 Janczyk, Pawel ...... M20 Goul, Claire ...... M103 Hess, Harold F...... A11 Jang, EunRyoung ...... E8 Grassart, Alexandre ...... E38 Hetzer, Martin W...... E110,E74,M124 Jang, Hyein ...... E8 Gray, Matt ...... E112 Heusinkveld, Lauren E...... M189 Jaqaman, Khuloud ...... E75 Greco, Valentina ...... A10 Higashi, Tomohito ...... M125 Jarvis, Erich D...... A1 Green, Amelia ...... M138 Higgs, Henry ...... M107 Jawerth, Louise ...... M220 Green, Marc ...... M26 Hinchcliffe, Edward H...... M25 Jegou, Antoine ...... M155 Green, Rebecca A...... E111,M8,M81 Hiscock, Tom ...... M138 Jensen, Grant J...... M212,S16 Greenberg, Roger A...... E108 Ho, Theodore T...... E105 Jensen, Gregory S...... M201 Grenfell, Andrew W...... M23 Hocky, Glen M...... E25,M161 Jeoung, Myoungkun ...... E8 Grewal, Savraj S...... E88 Hodgson, Louis ...... E56, M163 Ji, Weike ...... M107 Griffiths, Howard...... M101 Hoekstra, Jake ...... M214 Jian, Youli ...... M131 Grishchuk, Ekaterina L...... M184 Hoffman, Brenton D...... E33 Jiang, Qing ...... M66 Groen, Aaron C...... M151 Höing, Susanne ...... M79 Joglekar, Ajit P...... A6,E115 Grunwald, David ...... M97 Holland, Andrew ...... E114 Johannes, Ludger ...... E15 Guck, Jochen ...... M102 Hollenberg, Morley D...... E9 Johansson, Staffan ...... M123 Guetta-Terrier, Charlotte ...... M159 Hollyer, Ian ...... M78 John, Karin ...... M46 Guitierrez, Abraham ...... M208 Holt, Liam J...... M150 Johnson, Natalie K...... M41 Gujral, Taran ...... E84 Holtzer, Laurent ...... M129 Jones, Liesl B...... M58 Gunning, Peter W...... E17,E31,E95 Holzbaur, Erika L...... E44,M104 Jones, Patrick ...... E5 Guo, Shunling ...... E10,M152 Hong, SunHae ...... M39 Jonikas, Martin C...... M101 Guo, Syuan-Ming ...... E36 Hopman, Mark ...... M97 Jordan, Holly A...... M60 Gupta, Deepesh K...... M123 Horne-Badovinac, Sally ...... M226 Jorgensen, Erik ...... M34 Gurel, Pinar ...... E3 Horwitz, Alan R...... E123 Joseph, Aviva ...... M97 Gurung, Ritu ...... E94 Hoshino, Daisuke ...... E42,M5 Joyner, Ryan P...... M100 Guttierez, J.Ignacio ...... M150 Houser, Justin R...... M38 Julicher, Frank ...... M129 Güttler, Thomas ...... M151 Housman, Max ...... M202 Jullié, Damien ...... E104 Gutzman, Jennifer H...... E86 Howard, Jonathon ...... M86 Gygi, Steven P...... M151 Hsieh, Chung-Han ...... M215 Hsieh, Wan-Ying ...... E82 Hu, Chi-Kuo ...... M227 K Hu, Shiqiong ...... E125 Kachaner, David ...... M183 H Hu, Shuiqing ...... E18 Kaiser, Chris A...... M59 Hacker, William ...... E120 Hu, Xindao ...... E29 Kaksonen, Marko ...... M207,M35 Hahn, Klaus M...... M87,E48 Huang, Chiu-Hui ...... M214 Kale, Shiv D...... M17 Haimovich, Gal ...... E106 Huang, Fang ...... M50 Kalyanaraman, Vani ...... M88 Hall, Alan ...... M168 Huang, Fei ...... E30 Kambara, Taketoshi ...... M48 Hamada, Nanako ...... E51 Huang, Kerwyn C...... M194 Kamranvar, Siamak A...... M123 Hamasaki, Maho ...... M134 Huang, Sui ...... M30 Kanamori, Toshiyuki ...... M113 Hameed, Feroz M...... E124 Huang, Xiahe ...... M131 Kantzler, Courtney L...... M17 Hamilton, Eric S...... M201 Huet, Guillaume ...... E93 Kapoor, Tarun M...... M28,M68,M85 Hamilton, Nicholas A...... M164 Hughey, Jacob ...... M191 Karginov, Andrei V...... M89 Hammarlund, Marc ...... M171 Huisman, Maximillian ...... M97 Karlen, Karin L...... E12 Han, Sangyoon J...... E123 Humphries, Martin J...... E92 Kassambara, Alboukadel ������������������������������������E69 Han, TaeHee ...... M223 Hurley, James ...... S16 Kato, Ryuji ...... M113 Han, Weiping ...... E17 Hurrell, Tracey ...... M144 Keasling, Jay D...... M74 Hancock, William O...... E46 Huyot, Vincent ...... M89 Keber, Felix C...... M44 Handly, Linda N...... M186 Hyatt, Dylan M...... M75 Kee, Anthony J...... E17 Hanna, Michael ...... M37 Hyman, Anthony A...... S10,E26,M122, Keizer-Gunnink, Ineke ...... M198 Hannezo, Edouard ...... E87,M160 M148,M220 Keller, Lani C...... M56 Hänninen, Pekka ...... E92 Hyrskyluoto, Alise ...... E93 Keller, Ray ...... E90 Hanson, Lindsey A...... M39 Kelley, Charlotte F...... M40 Hao, Xiaojiang ...... M131 Kennedy, Kristopher J...... M150 Hardeman, Edna C...... E17,E95 Kennedy, Scott R...... M214 Hariadi, Rizal F...... M49 I Kenny, Sam J...... M146,M203 Hariharan, Iswar K...... M221 Inoue, Takanari ...... M117 Keren, Kinneret ...... M141 Harker, Alyssa J...... M161 Insinna, Christine ...... E60 Kerkhoff, Eugen ...... M199 Harmon Hines, Deborah ���������������������������������������A2 Irastorza, Ibai ...... M207 Kern, David M...... M63 Harper, J. W...... M214 Irianto, Jerome ...... E108 Kestner, Cortney A...... M24 Harris, J. A...... M195 Irudayaraj, Joseph ...... E112 Ketcham, Stephanie A...... M79 Harris, Robin E...... M221 Ishihara, Keisuke ...... M151 Ketova, Tatiana ...... M5 Keymer, Juan E...... E22 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 167 ABSTRACT AUTHOR INDEX
Khaliullin, Renat ...... M8 Leander, Rachel ...... M189 Luo, Jia ...... M66 Khan, Meraj H...... E92 LeBorgne-Rochet, Mailys ...... M162 Luo, Kathy Q...... E52 Kim, DaeJoong ...... E78 Lechtreck, Karl F...... M195 Luo, Lin ...... M93 Kim, HyeYoung ...... E89 Lecuit, Thomas ...... M136 Luxton, G.W.Gant ...... E76,M197,M86 Kim, Philip M...... M90 Leda, Marcin ...... M121 Kim, Raehyun ...... M70 LeDour, Caroline ...... E70 Kim, YeunJu ...... M72 Lee, AhYoung ...... M22 Kim, Yumi ...... M29 Lee, Intaek ...... M206 M Ma, Jian ...... M31 King, Megan C...... E107 Lee, Jaclyn S...... E101 Ma, Le ...... E32 Kini, Shloka ...... M59 Lee, Jason E...... M106 Ma, Minghe ...... M36 Kintner, Christopher ...... E90 Lee, Kyu J...... E9 Ma, Shan ...... M114 Kirchhausen, Tom ...... E15,M180,M36 Lee, Robin E...... M190 Mackinder, Luke C...... M101 Kirschner, Marc W...... E84,M151 Lee, Tina H...... M209 Macquart, Coline ...... E70 Kittisopikul, Mark ...... E75 Lefebvre, Matthew F...... M143 Maddox, Paul S...... E117 Klar, Sascha ...... E126 Leffler, Hakon...... E13 Madhani, Hiten D...... A8 Klebl, Bert ...... M79 Legant, Wesley R...... M169,M180, Maejima, Ikuko ...... M134 Klein, Bernard ...... E69 M47,M68 Magon, Monika S...... M127 Kleiner, Ralph E...... M28 Leland, Bryan A...... M56 Mahadevan, L...... M138 Klitzing, Haley A...... M70 Lelievre, Sophie A...... E112 Mahajan, Divyanshu ...... M210 Klomp, Jennifer B...... M89 Lemaitre, Regis ...... M148 Maiato, Helder J...... E28 Klose, Christian ...... E19 Lemanski, Larry F...... M118 Maksaev, Grigory ...... M201 Kner, Peter ...... M195 Lemire, Joan ...... M142 Malengo, Gabriele ...... M126 KnotheTate, Melissa L...... E31 Lénárt, Péter ...... E66,M156, Malhotra, Vivek ...... M204 Kochegarov, Andrei ...... M118 M181 Malik, Asrar B...... E30 Koga, Nobuyasu ...... E6 Lenne, Pierre-François ...... M136 Malinovska, Liliana ...... M102 Köhler, Simone ...... M29 Lerit, Dorothy A...... M60 Mandigo, Torrey ...... E72 Kohrman, Abraham Q...... M6 Leung, Edison ...... E4,E64 Mangeol, Pierre J...... M80 Kolber, Benedict J...... M56 Levental, Ilya ...... E19,M77 Manning, MLisa ...... E34 Kollman, Justin M...... M193 Levental, Kandice R...... E19,M77 Manor, Uri ...... M7 Komarova, Yulia A...... E30 Levin, Michael ...... M142 ManuelGarcia-Verdugo, Jose ���������������������������E101 Komeili, Arash ...... M212,S16 Levorse, John ...... M230 Mao, Yinghui ...... M96 Kondo, Shigeru ...... S15 Lewis, George K...... M173 Marcus, Adam ...... M2 Konen, Jessica M...... M2 Li, Cheng ...... M210 Maridonneau-Parini, Isabelle ��������������������������������E7 König, Julia ...... M122 Li, Deyu ...... E1 Marinkovich, Peter ...... E53 Koo, Peter ...... E107 Li, Dong ...... M169 Marino, Eric ...... M180 Koren, Amnon ...... E113 Li, Rong ...... A4 Marjon, Kristopher D...... E12 Kortholt, Arjan ...... M198 LI, Ting ...... M131 Markmiller, Sebastian J...... M99 Kosak, Steven T...... M32 Li, Wan ...... M146 Marko, John F...... M95 Koshland, Douglas E...... M115 Li, Yan ...... S17 Markossian, Sarine ...... M18 Kosmalska, Anita ...... E121 Li, Yang ...... M131 Marks, Jessica R...... M39 Kostow, Nora ...... M29 Li, Yuan ...... M130 Marshall, Wallace F...... M14 Kourtidis, Antonis ...... E85 Li, Yueming ...... M176 Martens, Allison M...... E119 Kovar, David R...... E25,M161, Lie, Richard ...... M55 Martin, Adam ...... M137,M167 M199 Lieu, Zi Zhao Robert ...... E124 Martin, Sophie G...... M15 Kozminski, Keith G...... M75 Lilley, Kathryn S...... M144 Martina, Jose A...... M92 Kraft, Mary L...... M70 Lim, Jeong-A ...... M92 Martinez-Arias, Alfonso ...... M144 Kremneva, Elena ...... E92 Limozin, Laurent ...... E122 Masai, Kaori ...... M71 Krndija, Denis ...... E87 Lin, Mei-Yao ...... E63,M216 Masedunskas, Andrius ...... E17 Kruse, Kevin J...... E30 Lin, Xubo ...... M77 Mason, Frank M...... M167 Kucharavy, Andrei ...... A4 Lindblom, Jael ...... M179 Mast, Fred D...... E62 Kühlbrandt, Werner ...... M33 Linder, Stefan ...... E7 Mathew, Joseph K...... E6,M69 Kuhn, Jonathan A...... E116 Linke, Marco ...... M166 Matos, Irina ...... M230 Kuhn, Richard J...... E99 Lippincott-Schwartz, Jennifer ...... E20,M7,M169, Matsubayashi, Yutaka ...... M9 KuhnCuellar, Luis ...... M147 M211,M223 Matsui, Hirofumi ...... M113 Kulesa, Tony ...... E36 Lisa, Weingarten ...... M59 Matus, David Q...... M6 Kulukian, Anita ...... E114 Liu, Chenshu ...... M96 Mayo, Alyssa M...... M184 Kwas, Constance ...... E86 Liu, Haijiao ...... M231 Mayor, Roberto ...... S4 Liu, Jing ...... E112 Mayor, Satyajit ...... E6,M132,M69 Liu, Kai ...... M131 McAlister, Graeme C...... M151 Liu, Limin ...... M24 McCaffery, J.Michael ...... M105 L Liu, Shiwei ...... M27 LaBarge, Mark A...... E65 McClellan, Mark ...... M86 Liu, Tsung-Li ...... M180 Lacal, Jesus ...... M198 McClelland, Erin E...... M189 Liu, Xuezhao ...... M131 LaCroix, Andrew S...... E33 McConnell, Michael J...... E77 Liu, Yi ...... M195 Ladouceur, Anne-Marie ...... E117 McCoy-Simandle, Kessler ��������������������������������� M58 Liu, Youtao ...... M198 Ladoux, Benoit ...... M159 McCroskey, Scott ...... A4 Livshitz, Anton ...... M141 Lafer, Eileen M...... M38 McDowell, Gary S...... M142 Lobingier, Braden ...... M208 Lagache, Thibault ...... E38 McGuire, Allister ...... E10 Loftus, Patrick D...... M179 Lahav, Galit ...... M187 McKenzie, Andrew J...... E42 Logarinho, Elsa ...... M17 Lakkaraju, Aparna ...... E68 McKim, Kim S...... E118 Loiseau, Etienne ...... M44 Lamason, Rebecca ...... E102 McMahon, Moira ...... M22 Long, Hongyan ...... M159 Lambert, Talley ...... E6 McVicker, Derrick P...... E29 Lord, Matthew J...... E25 Lampson, Michael A...... A7,M184,M21 McWhirter, Rebecca D...... M171 Lord, Samuel ...... M196 Lanman, Jason K...... E99 Medalsy, Izhar ...... E18 Lorent, Joseph H...... E19,M77 Laos, Triin ...... M109 Medina, Christopher S...... M170 Losick, Richard ...... M12 Laplante, Caroline ...... M50 Megason, Sean G...... M138,M180 Loubéry, Sylvain ...... M129 Lappalainen, Pekka ...... E92 Megraw, Timothy L...... M61 Lu, Dan ...... M178 Lari, Azra ...... M97 Mehsen, Haytham ...... M183 Lu, Lei ...... M210 Larionov, Vladimir ...... M18 Mehta, Shalin ...... E6 Lu, Quanlong ...... E60 Larouche, Myreille ...... M183 Mela, Ioanna ...... M37 Lu, Shaoying ...... M91 Larson, Andrew R...... M228 Mellinghoff, Ingo K...... E82 Lu, Wen ...... M84 Lasker, Keren ...... M10 Melville, David B...... E57 Lucas, Christine A...... E17 Lawrimore, Joshua G...... E117 Meng, Zhaojing ...... E60 Lucast, Louise ...... M71 Lazarova, Zelmira ...... E53 Mengistu, Meron ...... M173
168 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting ABSTRACT AUTHOR INDEX
Meraldi, Patrick ...... M67 Narasimha, Maithreyi ...... M139 Pare, Jean-Francois ...... M142 Mertz, Aaron F...... M230 Narayanaswamy, Pradeep �������������������������������� M71 Park, Bridget S...... M211 Merz, Alexey J...... M208 Natarajan, Meenubharathi ��������������������������������E125 Park, Danielle ...... M124 Mesa, Kailin R...... A10 Nayak, Jayakar V...... M229 Park, Demian ...... E36 Messelaar, Emily ...... M40 Nazarko, Taras Y...... E100 Parker, Laurie ...... E112 Mettler-Altmann, Tabea ...... M101 Neal, Michael S...... M118 Paschal, Bryce M...... E77 Meyer, Moritz T...... M101 Nechemia-Arbely, Yael ...... M22 Passegue, Emmanuelle ...... E105 Meyerson, Matthew L...... M27 Nedelec, François ...... M156 Patel, Avinash ...... M220 Midtvedt, Daniel ...... M102 Needleman, Daniel J...... E43 Patel, Tushar ...... E85 Mierzwa, Beata ...... M122 Neibart, Shane S...... E33 Patton, James G...... E42 Miga, Karen H...... M22 Nelles, David A...... M99 Peel, Nina ...... E26 Mihelc, Elaine ...... E99 Nelson, Celeste M...... M1 Pe’er, Dana ...... M185 Mijalkovic, Jona ...... M80 Nelson, David E...... M189 Peifer, Mark ...... E83,M60 Miller, Ann L...... M121,M125 Nemhauser, Jennifer ...... M16 Pelaez, Nancy ...... M54 Miller, Callie ...... M137 Nicholls, Peter K...... M63 Pellman, David S...... M27,M82 Miller, Leslie R...... E62 Nichols, Heddie L...... E9 Pereira, Antonio J...... E28 Miller, Yury I...... E100 Nickerson, Daniel P...... M208 Pérez-González, Carlos ...... E121 Miller III, David M...... M171 Niessen, Carien M...... M222 Peshkin, Leonid ...... M151 Miller-Jensen, Kathryn ...... M190 Niethamer, Terren K...... M228 Peterman, Erwin J...... M80 Mimori-Kiyosue, Yuko ...... M47 Nightingale, Daniel J...... M144 Peterson, Laura ...... M184 Ming, Monica ...... E9 Nilsson, Ulf J...... E13 Pfeffer, Stefan ...... M147 Mitchell, Lena ...... M118 Nishimura, Satoshi ...... M172 Pfeifer, Charlotte R...... E108 Mitchell, Madeline C...... M101 Niwa, Maho ...... M182 Phandthong, Rattapol ...... E37 Mitchison, Timothy J...... M148,M151, Niwa, Shinsuke ...... M83 Pi, FuWei ...... E122 M193 Nixon-Abell, Jonathon ...... M169 Picco, Andrea ...... M35,M207 Miyano, Masaru ...... E65 Nordenfelt, Pontus ...... E6 Pickrell, Alicia M...... M214 Miyata, Makoto ...... M192 Noree, Chalongrat ...... M116 Piel, Matthieu ...... E3 Mizrak, Arda ...... M178 Normandin, Karine ...... M183 Pigino, Gaia ...... E47 MochlyRosen, Daria ...... E11 Nunnari, Jodi ...... M105 Pignatelli, Jeanine ...... E56 Mochrie, Simon ...... E107 Nüske, Elisabeth ...... M102 Pilko, Anna ...... M186 Moerner, William E...... S3 Nussenzweig, Andre ...... E3 Pina-Nunez, Francisco ...... M182 Mofrad, Mohammad ...... E98 Pinch, Peter ...... M59 Mogilner, Alex ...... M159,S17 Piotrowski, Alexandra S. �������������������������������������� M1 Molloy, Kelly R...... M28 Planchon, Damien ...... M162 Monfort, Elena ...... M116 Pleiss, Jeff ...... M133 Monnier, Nilah ...... E66 O Plemel, Rachael ...... M208 Oakes, Patrick W...... M166 Montpetit, Ben ...... M97 Plitzko, Jürgen M...... M147 Obara, Christopher J...... M169 Monzo, Pascale ...... M159 Plooster, Melissa ...... M86 Ochoa, Stacy D...... M8,M81 Moore, Andrew S...... M104 Plutoni, Cedric ...... M162 O’Connell, Kevin F...... E26 Moore, Travis I...... E6 Polak, Lisa ...... M230 O’Connell, Mitchell R...... M99 Mor, Amir ...... M98 Polevoy, Gordon ...... M76 Odorizzi, Greg ...... M41 Moreaux, Jérome ...... E69 Polka, Jessica K...... M193 Oegema, Karen ...... E111,M19,M8, Morgan, David O...... M178 Poll, Brian ...... M32 M81 Morganthaler, Alisha N...... M161 Pollard, Luther W...... E25 Okada, Yasushi ...... M48 Mori, Masashi ...... E66,M156, Pollard, Thomas D...... M50 Oktay, Maja ...... E56 M181 Polley, Danny ...... E9 Oldenbroek, Marieke ...... E79 Morin, Nathalie S...... M162 Popa, Gabriel ...... E8 Oldfield, Richard ...... E31 Morishita, Rika ...... E51 Poser, Ina ...... M122 Oldrini, Barbara ...... E82 Morita, Masahiko ...... M47 Poulton, John S...... M60 Olivo-Marin, Jean-Christophe �����������������������������E38 Morrison, Deborah ...... E60 Pouwels, Jeroen ...... E92 On, Vincent ...... E37 Mosaliganti, Kishore R...... M138,M180 Presler, Marc ...... M151 Onami, Shuichi ...... E80 Mothes, Walther ...... M190 Prevo, Bram ...... M80,M81 ONeill, Audrey K...... M228 Moulding, Dale ...... M155 Pringle, John R...... M200 O’Neill, Patrick R...... M88 Mourier, Arnaud ...... M105 Priya, Rashmi ...... M164 Ong, Qunxiang ...... E10,M152 Muchir, Antoine ...... E70 Probst, Dimitri ...... M166 Onishi, Masayuki ...... M200 Mueller, Irina A...... M103 Pronobis, Mira ...... E83 Oostdyk, Luke T...... E77 Mueller, Joachim D...... E76 Prunuske, Amy J...... M56 Ordureau, Alban ...... M214 Mühleip, Alexander W...... M33 Puertollano, Rosa ...... M92 Oria, Roger ...... E121 Mühlhaus, Timo ...... M101 Punzo, Claudio ...... M114 Osawa, Masaki ...... M202 Muliyil, Sonia ...... M139 Pyrpassopoulos, Serapion �������������������������������� M43 Ostap, E. M...... M43 Mulla, Wahid A...... A4 Ostrem, Jonathan M...... E61 Müller-Reichert, Thomas ...... M122 Osunbayo, Olaolu ...... E45 Mullins, R. D...... M196 Oswald, Felix ...... M80 Mulvey, Claire M...... M144 Q Otey, Carol A...... E78 Qu, Qianhui ...... M20 Mund, Markus ...... M35 Ott, Carolyn M...... M223 Quake, Stephen ...... E81 Munder, Matthias C...... M102 Otto, Oliver ...... M102 Quintin, Sophie ...... M81 Muñoz, JoseJ ...... M225 Ouspenskaia, Tamara ...... M230 Qvit, Nir ...... E11 Munoz-Moreno, Raquel ...... M98 Ouwehand, Anette ...... M97 Murugesan, Sricharan ...... M56 Overholtzer, Michael ...... M168 Muthukrishnan, Gayatri ...... M132 Muthusamy, Nagendran �������������������������������������E58 R Myers, Kenneth A...... E5 Rabadán, MAngeles ...... M168 P Raben, Nina ...... M92 Paciorkowski, Alex R...... E77 Rachubinski, Richard A. �������������������������������������E62 Page, David C...... M63 RacineThiam, Hawa ...... E3 N Pal, Kasturi ...... E9 Radford, Sarah J...... E118 Nachury, Maxence V...... E101,M46 Palacios, Isabel M...... E49 Rafelski, Susanne M...... M103 Nadkarni, Ambika ...... M153 Paliulis, Leocadia V...... E119 Ramey, Christopher J...... M52 Naegeli, Kaleb M...... E16 Pallesen, Leif ...... M101 Ramirez-SanJuan, Guillermina R. ����������������������E34 Nafizi, Jabiz ...... M222 Paluch, Ewa K...... M119,M155, Ranatunga, Nimna S...... M26 Nagata, Koh-Ichi ...... E51 M158 Rangamani, Padmini ...... E41 Nager, Andrew R...... E101 Pamula, Melissa C...... M68,M85 Rankova, Aneliya ...... E96 Naik, Anar ...... E26 Panjwani, Noorjahan ...... E13 Raote, Ishier ...... M204 Nakatsu, Fubito ...... M71 Papanikou, Effrosyni ...... M205 Rauzi, Matteo ...... M136 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 169 ABSTRACT AUTHOR INDEX
Ray, Anne-Marie ...... M89 Schekman, Randy W...... E57,M203 Sokolova, Olga ...... M40 Razavi, Shiva ...... M117 Schillers, Hermann ...... E18 Soldati, Thierry ...... E54 Rechtsteiner, Andreas ...... A9 Schmid, Sandra L...... M128 Sommer, Frederik ...... M101 Reck-Peterson, Samara L...... E59,M78 Schneider, Jochen A...... M44 Sondalle, Samuel B...... M30 Redd, Michael J...... M179 Schöler, Hans R...... M79 Song, Eli ...... M36 Redwine, William B...... M78 Schoonderwoert, Vincent T. ����������������������������� M154 Song, Zhiqin ...... M131 Reeves, Gregory ...... M101 Schoonmaker, Judith N...... M52 Soniat, Michael ...... E109 Regot, Sergi ...... M191 Schorr, Stefan ...... M213 Sonnett, Matthew ...... M151 Reid, Brian ...... S17 Schreiner, Sarah M...... E107,M56 Sood, Pranidhi ...... M14 Reiff, Sarah B...... M14 Schroda, Michael ...... M101 Sourjik, Victor ...... M126 Reis, Carlos R...... M128 Schroeder, Courtney M. �������������������������������������E61 Spaeth, Jason M...... E79 Ren, Bing ...... M22 Schroeder, Lena K...... E21 Spatz, Joachim P...... E126 Ren, Xuefeng ...... S16 Schroer, Trina A...... M79 Specht, Suzanne ...... E60 Renou, Christophe ...... M150 Schuldiner, Maya ...... M213 Specht, Tanja ...... M207 Rice, Luke M...... M45 Schultz, Richard M...... A7 Spektor, Alexander ...... M27 Richardson, Tiffany M...... M149 Schurdak, Mark ...... M112 Spiliotis, Elias T...... E103 Richon, Sophie ...... E87 Schwappach, Blanche ...... M213 Springer, Timothy A...... E6 Richters, Karl ...... E29 Schwartz, Matthew L...... M208 Squatrito, Massimo ...... E82 Ries, Jonas ...... M35 Schwarz, Ulrich S...... M166 Stachowiak, Jeanne C...... M38 Ritt, Daniel ...... E60 Schweizer, Nina ...... E28 Stamatov, Rumen ...... M184 Rizzoli, Silvio O...... M219 Sedzinski, Jakub ...... M160 Stampfer, Martha ...... E65 Robinson, Douglas N...... M117 Seemann, Joachim ...... M65 Stanishneva-Konovolova, Tatyana �������������������� M40 Roca-Cusachs, Pere ...... E121,M225 Segura-Pena, Dario ...... M184 Stanley, Geoff ...... E81 Rodal, Avital A...... M175,M40 Seidel, Chris ...... A4 Staunton, Jack R...... M140 Rodriguez, Marbelys ...... M11 Seigler, Michael D...... E9 Stefan, Chris ...... M133 Rog, Ofer ...... M29 Sekine, Yuichi ...... M171 Stehn, Justine R...... E95 Roh, Minna ...... E56 Sen, Maya ...... M68 Steinman, Theodore I...... E55 Rohwer, Forest ...... S8 Sengupta, Kheya ...... E122 Stepanek, Ludek ...... E47 Rojas, Enrique R...... M194 Sengupta, Prabuddha ...... E20 Stephens, Andrew D...... M95 RomanoSpica, Natalia ...... E24 Sengupta, Ranjan ...... E99 Stephenson, Rachel E...... M125 Romeo, Yves ...... M155 Serbus, Laura R...... S6 Sterneckert, Jared L...... M79 Romet-Lemonne, Guillaume ��������������������������� M155 Serpe, Mihaela ...... M223 Stewart-Ornstein, Jacob ...... M187 Rosen, Michael K...... M199,S9 Setiawan, Linda ...... M221 Stitt, Mark ...... M101 Rosenberg, Scott C...... M29 Seum, Carole ...... M129 Stoiber, Marcus ...... E65 Rosenblatt, Jody ...... M179 Shaltouki, Atossa ...... M215 Stokasimov, Ema ...... M82 Rosenmund, Christian ...... M34 Shamir, Eliah R...... M3 Stone, Elisa M...... M57 Rossouw, Ammeret ...... M97 Shams, Hengameh ...... E98 Stow, Jennifer L...... M93 Rost, Benjamin ...... M34 Shapiro, Lucy ...... M10 Stramer, Brian M...... M9 Roth, Robyn ...... M101 Sharma, Ved P...... E35,E4,E64 Streichan, Sebastian J...... M143 Roth, Zak ...... E58 Shaw, James E...... E18 Strittmatter, Stephen M...... M171 Rothenberg, Katheryn E. ������������������������������������E33 Sheetz, Michael P...... E124 Strome, Susan ...... A9 Rothman, James E...... M206 Shelley, Michael J...... E43 Strzelecka, Magdalena ...... M23,S14 Rougerie, Pablo ...... E64 Shen, Jie-Pan ...... E39 Studer, Sean ...... E57 Roux, Aurélien ...... M122 Shen, Kang ...... M83 Stukenberg, P.Todd ...... M20,M24 Roux, Philippe P...... M155 Sheng, Zu-Hang ...... E63,M216 Stypula-Cyrus, Yolanda ...... M95 Roy, Jagoree ...... M90 Sherman, Michael B...... M38 Su, Kuan-Chung ...... E28 Rubinstein, Amir ...... M53 Sherwood, David R...... E16 Su, Xiaolei ...... M82 Rubinstein, Boris ...... A4 Shi, Liang ...... E58 Suarez, Cristian ...... M161 Rübsam, Matthias ...... M222 Shi, Ping ...... E8 Subramani, Suresh ...... E100 Ruby, Graham ...... M14 Shimi, Takeshi ...... E75 Subramanian, Poorna ...... M212,S16 Rusan, Nasser M...... E83,M60 Shirasu-Hiza, Mimi ...... E24 Sudhof, Thomas ...... E81 Rutledge, Samuel D...... M17 Shivashankar, G.V...... M94 Sugiura, Sinji ...... M113 Shook, David ...... E90 Sulkowski, Mikolaj J...... M223 Shraiman, Boris ...... M143 Sullivan, William T...... S6 Shreiber, David I...... M177 Sun, Lu ...... M92 Shtengel, Gleb ...... A11 Sun, Yanan ...... M130 S Shuman, Henry ...... M43 Sun, Yaohui ...... S17 Saba, Nakhle S...... M18 Shun, TongYing ...... M112 Sun, Ying ...... E30 Sabatinos, Sarah A...... M26 Sideris, Dionisia P...... M214 Sun, Yuxin ...... S17 Sahai, Erik ...... M124 Siefert, Joseph C...... E113 Sung, BongHwan ...... M5 Sahu, Srishti U...... E86 Siegel, Richard ...... E20 Sunyer, Raimon ...... M225 SaitoReis, Chelsea A...... E12 Silver, Pamela A...... M193 Surma, Michal A...... E19 Salbreux, Guillaume ...... M44,M119, Sim, Janice H...... E115 Surovtsev, Ivan V...... M150 M158 Simmons, Craig ...... M231 Swaminathan, Vinay ...... E6 Saleem, Ramsey A...... E62 Simon, Anthony ...... E87,E91 Sweet, Matt J...... M93 Salogiannis, John ...... E59 Singer, Robert H...... E106,E56 Swinburne, Ian A...... M138 Salomaa, Siiri ...... E92 Sirka, Katarina ...... M3 Szuhai, Karoly ...... M124 Sanders, Joseph ...... E4 Sivakumar, Sushama ...... M20 Sansam, Christopher L...... E113 Sivaramakrishnan, Sivaraj ��������������������������������� M49 Santoro, Francesca ...... E10 Skau, Colleen T...... E3 Santos, Antonio J...... M204 Skinkle, Allison ...... E19 T Saravanan, Surat ...... M139 Tabata, Hidenori ...... E51 Skruzny, Michal ...... M126 Sarro-Schwartz, Aliyah Y. �����������������������������������E10 Takayama, Jun ...... E80 Slabodnick, Mark ...... M14 Sathe, Mugdha ...... M132 Talbot, Prue ...... E37 Slade, Andrea L...... E18 Satpute-krishnan, Prasanna ���������������������������� M211 Tam, Arvin B...... M182 Slone, Samuel ...... E14 Saul, Joshua ...... M221 Tamura, Masato ...... M113 Smindak, Richard J...... M75 Saunders, Cosmo A...... E76,M197 Tan, LiXuan ...... E68 Smith, Carlas ...... M97 Sauvonnet, Nathalie ...... E38 Tang, Guihua ...... M131 Smith, Deanna ...... E58 Sawaguchi, Akira ...... M172 Tang, Jeffrey H...... M100 Smith, Elizabeth M...... E76 Scarcelli, Nikolas ...... M118 Tang, Vivian ...... M153 Smith, Lydia ...... E117 Scarpa, Margherita ...... M204 Tani, Tomomi ...... E6 Smith, Mathew ...... M119 Schaedel, Laura ...... M46 Tanner, Kandice ...... M140 Smith, Matthew B...... M158 Schaefer, Andrew W...... M154 Tanner, Lukas B...... M71 Snow, Chelsi ...... E77 Schaffer, Miroslava ...... M147 Tapia, Hugo ...... M115 Sohn, Mira ...... M72 170 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting ABSTRACT AUTHOR INDEX
Taylor, D. L...... M112 Vershinin, Michael ...... E45 Wolfenson, Haguy ...... E124 Taylor, Nicole ...... E40 Viana, Matheus P...... M103 Wollman, Roy ...... M186 Tebbs, Irene R...... M50 Vidi, Pierre-Alexandre ...... E112 Wolyniak, Michael J...... M56 Tellkamp, Frederik ...... M222 Vignjevic, Danijela M...... E87,E91 Wong, Victor C...... M190 Teranishi, Hirofumi ...... M134 Viita, Tiina ...... E93 Wong, Yvette C...... M104 Termini, Christina M...... E12 Vijayakumar, Soundarapandian ��������������������������E53 Wood, Ashley M...... M32 Terry, Stephen J...... E23 Viovy, Jean-Louis ...... E91 Woolums, Brian M...... M197 Teruel, Mary N...... M188 Viplav, Abhiyan ...... M219 Worman, Howard J...... E70 Thattai, Mukund ...... M132 Virtanen, Jori ...... E93 Wu, Di ...... M81 Theriot, Julie A...... M194 Visetsouk, Mike R...... E86 Wu, Fabai ...... E22 Théry, Manuel ...... M46 Vitre, Benjamin D...... E114 Wu, Shu-Zon ...... E97 Thibault, Sarah ...... E119 Vladar, Eszter K...... M229 Wühr, Martin ...... M151 Thompson, E A...... E85 Voeltz, Gia ...... M106 Wunder, Cristian ...... E15 Thrasher, Adrian J...... M155 Vogel, Stephen M...... E30 Wynn, R.Max ...... M65 Ti, Shih-Chieh ...... M85 Vogts, Angela ...... M219 Tianzhi, Luo ...... M117 vonDassow, George ...... M121 Tie, HiengChiong ...... M210 vonSchie, Sabine ...... M188 Ting, Alice Y...... M145 VonZastrow, Mark E...... E104 X Xia, Jingjing ...... M222 Tiryaki, Volkan M...... M177 Vorhagen, Susanne ...... M222 Xia, Lijun ...... E13 Titus, Margaret A...... M197 Xiao, Lin ...... E78 Tkach, Karen ...... M188 Xie, Shicong ...... M167 Toops, Kimberly A...... E68 Xie, Yuxiang ...... E63 Torres, Vicente E...... E53 W Wagner, Elizabeth ...... M120,M166 Xing, Jian ...... M62 Torta, Federico ...... M71 Waldmann, Herbert ...... M79 Xu, Ke ...... M146,M203 Tour, Ella ...... M55 Walia, Vijay ...... E60 Xu, Meng ...... M131 Toyama, Brandon H...... E110 Walker, Graham ...... M59 Xue, Alice ...... E64 Tran, Jospeh ...... E75 Wall, Adam A...... M93 Tranter, Michael ...... E14 Wallace, Darren P...... E53 Trepat, Xavier ...... E121 ,M162, Wallingford, John B...... M160 M225 Walsh, Rylie B...... M175 Y Treutlein, Barbara ...... E81 Yadav, Rahul ...... E94 Wang, Aibing ...... M157 Trimbuch, Thorsten ...... M34 Yagisawa, Fumi ...... M182 Wang, Chen ...... M30 Troeste, Melissa ...... E78 Yamada, Justin ...... M105 Wang, Hui ...... E48,M87 Truckenbrodt, Sven ...... M219 Yamamoto, Ami ...... M86 Wang, Peipei ...... M159 Trujillo, Caleb M...... M54 Yamashita, Norio ...... M47 Wang, Peng ...... M183 Truongquang, Binhan ...... M119 Yan, Irene K...... E85 Wang, PeterYingxiao ...... M91 Tsai, Hung-Ji ...... A4 Yanagisawa, Masumi ...... M113 Wang, Shaohe ...... E111,M8,M81 Tseng, Yun-Yu ...... M168 Yang, Biying ...... M66 Wang, Shiwei ...... E63 Tsimring, Lev ...... E67,M174 Yang, Bo ...... E124 Wang, Xiaochen ...... M130 Tu, Fan ...... M160 Yang, Chen ...... M131 Wang, Xin ...... M130 Tubbs, Anthony ...... E3 Yang, Chonglin ...... M130,M131 Wang, Xinnan ...... M215 Tuzel, Erkan ...... M43 Yang, Chun-Song ...... E77 Wang, Yarong ...... E56,E64 Tworoger, Michael ...... M167 Yang, Karren ...... A7 Wang, Yingchun ...... M131 Tyler, Jolien S...... M62 Yang, Pinfen ...... M195 Wang, Yuchuan ...... M31 Tymanskyj, Stephen ...... E32 Yang, Renzhi ...... M108 Wang, Yu-li ...... E2 Yap, Alpha S...... M164 Wangsa, Darawalee ...... M17 Yates III, John ...... M24 Warr, Matthew R...... E105 Ye, Fan ...... E101 Wasserman, Steven A...... M40 U Yee, Michael C...... E9 Ulbricht, Elke ...... M102 Watanabe, Shigeki ...... M34 Yeh, Ting-Yu ...... M79 Umbreit, Neil T...... M27 Waterman, Clare M...... A11,E6,M157 Yelon, Deborah ...... M4 Upadhyayula, Srigokul ...... E15,M180 Waterman, Clare M...... E3 Yeo, Gene ...... M99 Weaver, Alissa M...... E42,M5 Ying, Michael ...... S17 Webb, Donna J...... E1 Yoder, Bradley K...... E53 Weber, Peter K...... M70 Yokota, Hideo ...... M47 Webster, Micah T...... M7 Yonis, Amina ...... M155 V Weems, Lauren ...... A4 Vaghela, Malti ...... M155 Yoshimori, Tamotsu ...... M134 Wei, Ho-Chun ...... M76 Vahey, Michael D...... M193 Youle, Richard J...... M214,M217 Wei, Jen-Hsuan ...... M65 Vaidya, Nilesh ...... E40, M149 Young, Jennifer L...... E126 Weidmann, Maxwell D...... E35 Vale, Ronald D...... E61 Yu, Hongtao ...... M20 Weigel, Aubrey V...... M169 VanDerBliek, Alexander M. ������������������������������ M217 Yu, Jiun-Yann ...... M62 Weis, Karsten ...... M100,M97 vanderWall, Elsken ...... M124 Yuan, Ji-Ping ...... M26 Weissman, Jonathan S...... A3,M213 vandeVen, Robert ...... M124 Yuan, Lin ...... M203 Welch, Matthew D...... E102, G1 vanDiest, Paul J...... M124 Yue, Zhou ...... M159 Wenderoth, MaryPat ...... M51 vanDomselaar, Robert ...... M124 Wenk, Markus R...... M71 vanHaastert, Peter J...... M198 Wesolowska, Natalia ...... M181 VanHaren, Jeffrey ...... E48 West, Matthew ...... M41 vanSteensel, Bas ...... M31 Z Westlake, Christopher J. ������������������������������������E60 Zaburdaev, Vasily ...... M102 Varjosalo, Markku ...... E93 Whatley, Zakiya ...... M56 Zahedi, Atena ...... E37 Varner, Victor D...... M1 Wheeler, Richard ...... M220 Zahreddine, Ramzi N...... M62 Vartiainen, Maria K...... E93 White, Alex ...... M98 Zaritsky, Assaf ...... M168 Vasquez, Claudia ...... M137 White, Pam ...... S6 Zasadzinska, Ewelina ...... M24 Vaughan, Kevin T...... M25 Wick, Susan M...... M56 Zaytsev, Anatoly V...... M184 Vaughan, Megan ...... M4 Wieschaus, Eric ...... M143 Zeng, Cody ...... E10 Vaughn, Gregory ...... M118 Wigington, Callie P...... M90 Zenklusen, Daniel ...... M97 Vazquez, Gabriella ...... E72 Wilhelm, James E...... M116 Zerbib, Lital ...... M141 Veltman, Douwe M...... M198 Wilk, Ronit ...... M76 Zhang, Chuanmao ...... M66 Veneziano, Remi ...... E36 Williams, James ...... E4 Zhang, Jian ...... E2 Venkatesh, Aditya ...... M114 Wilson, Amy L...... E23 Zhang, Kai ...... E10,M152 Verde, Fulvia ...... M11 Winding, Michael ...... M84 Zhang, Liguo ...... M31 Verheyen, Esther M...... M223 Winey, Mark ...... M62 Zhang, Ruyan ...... E10 Verhulsel, Marine ...... E91 Winkelman, Jonathan D...... M161 Zhang, Wan ...... M6 Verma, Priyanka ...... M28,M68 Winsor, James ...... M209 Zhang, Xuezhi ...... E54 Verma, Suzie ...... M164 Wittmann, Torsten ...... E48 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 171 ABSTRACT AUTHOR INDEX
Zhang, Yang ...... M31 Zhou, Houjiang ...... M144 Zick, Michael ...... M208 Zhang, Zhengyang ...... M146 Zhou, Jing ...... E55 Zijlstra, Andries ...... M5 Zhang, ZiChao ...... M65 Zhou, Ming ...... E60 Zimmerberg, Joshua ...... M70 Zhao, Michael L...... M188 Zhu, Cheng ...... E121 Zimmerman, Amber J...... M170 Zhao, Min ...... S17 Zhu, Jie ...... M159 Zimmermann, Dennis ...... E25 Zhao, Wenting ...... M39 Zhu, Kan ...... S17 Zimmermann, Richard ...... M213 Zhao, Yao ...... E107 Zhu, Lu ...... M133 Zou, Wei ...... M130 Zhao, Zhiling ...... M8 Zhuang, Xiaowei ...... S2 Zulueta-Coarasa, Teresa ������������������������������������E50 Zheng, Yixian ...... E75 Zhuchenko, Olga ...... E54 Zunitch, Matthew J...... M175 Zhou, Bing ...... E63,M216 Zhuravlev, Yelena ...... E24
ABSTRACT author disclosures
The ASCB requires that audiences be informed of presenters’ (speakers, authors, and contributors) academic and professional affiliations, and disclosure of any significant financial interest or other relationship with the manufacturer(s) of any commercial product(s) discussed in a presentation. This policy allows the listener/attendee to be fully informed in evaluating the information being presented. Disclosure includes any relationship that may bias one’s presentation or which, if known, could give the perception of bias. These situations may include, but are not limited to: 1) stock options or bond holdings in a for-profit corporation or self-directed pension plan, 2) research grants, 3) employment (full-or part-time), 4) ownership or partnership, 5) consulting fees or other remuneration, 6) non-remunerative positions of influence such as officer, board member, trustee, or public spokesperson, 7) receipt of royalties, and 8) speakers bureau.
Author Disclosure–Institution
Belmont, Lisa Employment (full or part-time)-Genentech, Inc. Biro, Maté Research Grant-Cancer Australia Chen, Baohui Stock options or bond holdings in a for-profit corporation or self-directed pension plan-National Institute of Biological Sciences DeVico, Anthony Research Grant-Bill and Melinda Gates Foundation Fuchs, Elaine Research Grant-National Institute of Arthritis, Musculoskeletal and Skin Diseases Research Grant-New York State Stem Cell Initiative Gujral, Taran Research Grant-Ellison Foundation Grant Gunning, Peter Non-remunerative positions of influence such as officer, board member, trustee, or public spokesperson-Novogen Hannezo, Edouard Research Grant-Trinity College Hu, Shuiqing Employment (full or part-time)-Bruker Nano Surfaces Johansson, Staffan Research Grant-Medical Biochemistry and Microbiology, Uppsala University, Uppsala Sweden Klose, Christian Employment (full or part-time)-Lipotype Kondo, Shigeru Research Grant-JST Lewis, George Research Grant-Bill and Melinda Gates Foundation Li, Yuan Stock options or bond holdings in a for-profit corporation or self-directed pension plan-National Institute of Biological Sciences Lu, Shaoying Ownership or partnership-Cell E&G Ma, Lisa Employment (full or part-time)-Affymetrix eBioscience Medalsy, Izhar Employment (full or part-time)-Bruker Nano Surfaces Mengistu, Meron Research Grant-Bill and Melinda Gates Foundation Meyerson, Matthew Stock options or bond holdings in a for-profit corporation or self-directed pension plan-Foundation Medicine Patel, Avinash Stock options or bond holdings in a for-profit corporation or self-directed pension plan-Max Planck Institute of Molecular Cell Biology and Genetics Sedzinski, Jakub Research Grant-University of Texas Shaw, James Employment (full or part-time)-Bruker Nano Surfaces Slade, Andrea Employment (full or part-time)-Bruker Nano Surfaces Strzelecka, Magdalena Employment (full or part-time)-Qiagen Sullivan, William Research Grant-NSF Sun, Yanan Stock options or bond holdings in a for-profit corporation or self-directed pension plan-National Institute of Biological Sciences
172 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Author Disclosure–Institution
Surma, Michal Employment (full or part-time)-Lipotype Tu, Fan Research Grant-University of Texas Wallingford, John Research Grant-University of Texas Wang, Peter Yingxiao Ownership or partnership-Cell E&G Wang, Xiaochen Stock options or bond holdings in a for-profit corporation or self-directed pension plan-National Institute of Biological Sciences Wang, Xin Stock options or bond holdings in a for-profit corporation or self-directed pension plan-Institute of Genetics and Developmental Biology Yang, Chonglin Stock options or bond holdings in a for-profit corporation or self-directed pension plan-Institute of Genetics and Developmental Biology Zou, Wei Stock options or bond holdings in a for-profit corporation or self-directed pension plan-National Institute of Biological Sciences
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 173 Notes
174 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting Notes
The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting 175 Notes
176 The 2015 ASCB Annual Meeting l www.ascb.org/2015meeting
Learn greater control in cell culture using Cell-Ess® Serum Replacement
Cell-Ess® serum replacement for more relevant cell culture results
Performance Achieve consistent cell performance not attainable with FBS Convenience More time for discoveries. No more lot testing, heat inactivation or thawing Control Defined components. Animal component free. No variability
Visit us at Booth # 74 8 and attend our Tech Talk
Talk Title Variables in FBS Impact Cell Performance, Reproducibility and Relevant Experimental Results Date Tuesday Dec. 15, 2015 2:00 –2:45 PM Room Exhibit Hall, Theatre #2 Let it Grow
www.cell-ess.com | www.essentialpharma.com
ASCB_Program.indd 1 Cell-Ess Ad 1015 for ASCB Program 10/14/15 11:33 AM