New Testament Purple Dye
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Making Basic Period Pigments at Home
Making Basic Period Pigments at Home KWHSS – July 2019 Barony of Coeur d’Ennui Kingdom of Calontir Mistress Aidan Cocrinn, O.L., Barony of Forgotten Sea, Kingdom of Calontir Mka Holly Cochran [email protected] Contents Introduction .................................................................................................................................................. 3 Safety Rules: .................................................................................................................................................. 4 Basic References ........................................................................................................................................... 5 Other important references:..................................................................................................................... 6 Blacks ............................................................................................................................................................ 8 Lamp black ................................................................................................................................................ 8 Vine black .................................................................................................................................................. 9 Bone Black ................................................................................................................................................. 9 Whites ........................................................................................................................................................ -
Use of Orcein in Detecting Hepatitis B Antigen in Paraffin Sections of Liver
J Clin Pathol: first published as 10.1136/jcp.35.4.430 on 1 April 1982. Downloaded from J Clin Pathol 1982;35:430-433 Use of orcein in detecting hepatitis B antigen in paraffin sections of liver P KIRKPATRICK From the Department ofHistopathology, John Radcliffe Hospital, Headington, Oxjord OX3 9DU SUMMARY This study has shown that different supplies/batches of orcein perform differently and may fail. The "natural" forms generally performed better although the most informative results were obtained with a "synthetic" product. Orcein dye solutions can be used soon after preparation and for up to 7 days without the need for differentiation. After 10 days or so the staining properties become much less selective. Non-specific staining severely reduces contrast and upon differentiation overall contrast is reduced and the staining of elastin is reduced. Copper-associated protein positivity gradually fails and after 14 days is lost. For demonstrating HBsAg in paraffin sections of liver, it is best to use orcein dye preparations that are no older than 7 days and to test each batch of orcein against a known positive control. Orcein dye solutions are now commonly used for the was evaluated. detection of hepatitis B surface antigen (HBsAg) Eight samples of orcein were supplied by: Sigma copyright. and copper-associated protein in paraffin sections London Chemical ("natural" batch Nos 89C-0264 of liver.' It is generally believed that orcein dyes and 59C-0254 and "synthetic" batch Nos 31 F-0441); from a single source should be used. 2-6 Variable Raymond A Lamb ("natural" batch No 5094); results are obtained with different reagents perhaps Difco Laboratories ("natural" batch No 3220); because of different manufacturing procedures or BDH Chemicals ("synthetic" batch No 5575420A); significant batch variations. -
Chromosomal Staining Comparison of Plant Cells with Black Glutinous Rice (Oryza Sativa L.) and Lac (Laccifer Lacca Kerr)
© 2010 The Japan Mendel Society Cytologia 75(1): 89–97, 2010 Chromosomal Staining Comparison of Plant Cells with Black Glutinous Rice (Oryza sativa L.) and Lac (Laccifer lacca Kerr) Praween Supanuam1, Alongkoad Tanomtong1,*, Sirilak Thiprautree1, Somret Sikhruadong2 and Bhuvadol Gomontean3 1 Department of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen 40002, Thailand 2 Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Muang, Mahasarakham 44000, Thailand 3 Department of Biology, Faculty of Science, Mahasarakham University, Kantarawichai, Maha Sarakam, 44150, Thailand Received October 10, 2009; accepted February 8, 2010 Summary The study on chromosomal staining comparison of plant cells with natural dyes was carried out to compromise the use of expensive dyes. Dyes from black glutinous rice (Oryza sativa L.) and Lac (Laccifer lacca Kerr) were extracted using acetic acid, ethanol, butanol and hexane with the concentration levels of 30%, 45% and 60%, respectively. The pH was then adjusted from 1 to 7, the natural extracted dyes were used to stain the chromosomes of spider lily (Hymenocallis littoralis Salisb.) root cells, which were ongoing mitotic cell division, using the squash technique. The results showed that the natural extract dyes were capable of chromosome staining and cell division observing. Natural dyes which showed well-stained chromosome included 45% acetic acid-extracted black glutinous rice dye (pH 1–3), 45% butanol-extracted black glutinous rice dye (pH 1–3) and 60% ethanol-extracted Lac dye (pH 1–3). We also concluded that all other extracts have no significant quality as chromosomal staining indication. Key words Natural dye, Chromosome staining, Black glutinous rice (Oryza sativa L.), Lac (Laccifer lacca Kerr), Spider lily (Hymenocallis littoralis Salisb). -
Student Safety Sheets Dyes, Stains & Indicators
Student safety sheets 70 Dyes, stains & indicators Substance Hazard Comment Solid dyes, stains & indicators including: DANGER: May include one or more of the following Acridine orange, Congo Red (Direct dye 28), Crystal violet statements: fatal/toxic if swallowed/in contact (methyl violet, Gentian Violet, Gram’s stain), Ethidium TOXIC HEALTH with skin/ if inhaled; causes severe skin burns & bromide, Malachite green (solvent green 1), Methyl eye damage/ serious eye damage; may cause orange, Nigrosin, Phenolphthalein, Rosaniline, Safranin allergy or asthma symptoms or breathing CORR. IRRIT. difficulties if inhaled; may cause genetic defects/ cancer/damage fertility or the unborn child; causes damages to organs/through prolonged or ENVIRONMENT repeated exposure. Solid dyes, stains & indicators including Alizarin (1,2- WARNING: May include one or more of the dihydroxyanthraquinone), Alizarin Red S, Aluminon (tri- following statements: harmful if swallowed/in ammonium aurine tricarboxylate), Aniline Blue (cotton / contact with skin/if inhaled; causes skin/serious spirit blue), Brilliant yellow, Cresol Red, DCPIP (2,6-dichl- eye irritation; may cause allergic skin reaction; orophenolindophenol, phenolindo-2,6-dichlorophenol, HEALTH suspected of causing genetic PIDCP), Direct Red 23, Disperse Yellow 7, Dithizone (di- defects/cancer/damaging fertility or the unborn phenylthiocarbazone), Eosin (Eosin Y), Eriochrome Black T child; may cause damage to organs/respiratory (Solochrome black), Fluorescein (& disodium salt), Haem- HARMFUL irritation/drowsiness or dizziness/damage to atoxylin, HHSNNA (Patton & Reeder’s indicator), Indigo, organs through prolonged or repeated exposure. Magenta (basic Fuchsin), May-Grunwald stain, Methyl- ene blue, Methyl green, Orcein, Phenol Red, Procion ENVIRON. dyes, Pyronin, Resazurin, Sudan I/II/IV dyes, Sudan black (Solvent Black 3), Thymol blue, Xylene cyanol FF Solid dyes, stains & indicators including Some dyes may contain hazardous impurities and Acid blue 40, Blue dextran, Bromocresol green, many have not been well researched. -
The Prison Epistles”
A PASSAGE THROUGH THE NEW TESTAMENT Part Seven “The Prison Epistles” by J.S. Smith The Prison Epistles Author: Paul • 61-64 A.D. Ephesians The book is addressed to the saints in Ephesus, the capital of the Roman province of Asia in the first century. She was surrounded by mountains and the sea and intimate with Greek trade, art and science. Ephesus was the famed home of the Temple of Diana, one of the seven wonders of the world. The temple was 425 feet long by 225 feet wide and supported by 127 marble columns, 60 feet high. Ephesus was a point of travel convergence along a communication line stretching from Rome to the East. The church at Ephesus traces its start to the sixteenth chapter of the Acts of the Apostles. The Holy Spirit hindered Paul from speaking in Asia on his second missionary journey. After taking the gospel to Europe, Paul made a quick visit to Ephesus (Acts 18:19) with Priscilla and Aquila who stayed on there for several years after Paul’s departure. The author is the apostle Paul. In the spring of 54 A.D., Paul came back to Ephesus and joined Priscilla and Aquila. The purpose of this epistle does not seem to be occasioned by any special circumstances or outstanding event. Rather, it seems to be a convenient opportunity for paul to correspond with his beloved brethren at ephesus. A large part of this missive is about unity. Paul did not want to see or engender a split among jewish churches and Gentile churches. -
Lesson 49 Acts of the Apostles 14 – 28 Pattern Was to Enter a Community and Seek out the Local Synagogue
Lesson 49 Acts of the Apostles 14 – 28 pattern was to enter a community and seek out the local synagogue. There he, as a rabbinical visitor, would be asked to speak after first STUDY QUESTIONS treading from the sacred books. He lost no time in proclaiming that 1. Which apostle dominates the last part of Acts? What is his the long awaited Messiah had come, had died at the hands of the Jews background? See Galatians 1. What does Jesus say of him in Acts 9:15? and Romans, but had risen from the dead. He preached convincingly 2. Follow each missionary journey of Paul on the map so that the names that there was no other name under heaven by which a man could be and their locations become familiar. Atlas B32‐33 saved. This would invariably split the congregation. All would be 3. What is the controversy raised in the Jerusalem church because of astounded at this word, a few would believe, but after a day or two, Paul’s missionary success? How is it solved? vigorous opposition would arise. From city after city he was forced to 4. What is Paul’s pattern of evangelism when he enters a new town? flee. Sometimes he would leave Silas or Timothy behind to finish Compare Paul’s preaching in ch. 13 to the preaching of Peter 10:36‐48. organizing a church before they, too, were forced on. Isaiah 56:6‐8 or 49:1‐6 and prayer. The opening readings from Isaiah Initiation of Gentiles into the new Church had to be threshed stress that the Messiah will bring salvation to the ends of the earth out. -
The Sword of the Spirit the Sword of the Spirit
The Sword of The Spirit The Sword of The Spirit May, 2016 Saint Paul’s Church www.SaintPaulsBrookfield.com (203) 775-9587 There is plenty of more room in our growing family Transforming Lives Through Jesus A True Family looked, and there before me was a great multitude that no one could count, from every nation, tribe, people and language, standing be- fore the throne and before the Lamb. Vol. LXIII-No. 5 (Revelation 7:9) The Sword of the I Spirit was started in 1954 by the Rev. Dear Family of St. Paul's, A. Pierce Middleton n our diversity, our unity is in Christ. We are what the Bible I calls the household of God, a true family. Jesus redefined family by emphasizing the doing of God's will as the unifying force and determining sign of our tie that binds. As we worship the Lord, we capture a foretaste of a heavenly banquet awaiting us, one of transcendent unity, where our outward differences are drawn up into a oneness that only God can give. In our togetherness God is revealed, and here we begin to experience the coming of heaven even now. As God's divine tapestry continues to be woven in, through and among us, may we be that 'imago dei,' God's image, for all the world to see. There is plenty of more room in our growing family. May Christ's love continue to make us St. Paul’s Church one. 174 Whisconier Road Brookfield, Connecticut 06804 Faithfully, (203) 775-9587 www.SaintPaulsBrookfield.com The Rev. -
Degaulle Church of Christ
1 August 19, 2018 CHURCH OF CHRIST 4700 GENERAL DEGAULLE DRIVE NEW ORLEANS, LA 70131 www.DegaulleDriveCOC.com FOR JUST God will move heaven and earth rather than allow a SCHEDULE OF SERVICES soul to miss heaven. A case in point is the providence SUNDAY involved in the conversion of Lydia of Thyatira (Acts 16). Morning Bible Class 9:00 AM Providence refers to “that continuous activity of God Morning Worship 10:00 AM whereby He makes all the events (that happen in the Pew Packers 5:30 PM world) work out His purposes.”1 Paul wrote, “We know Evening Worship 6:00 PM ALLEN WEBSTER that all things work together for good to those who love MID-WEEK God, to those who are the called according to His purpose” (Romans 8:28). Tuesday Ladies Class 10:00 AM (September – May) Kingdoms, lives, and events cooperated unwittingly to bring about human Wednesday Bible Class 7:00 PM salvation (Galatians 4:4). Daily events are altered to reach people with the _____________________ gospel. If we saw the circumstances around us as God sees them, we would ELDERS doubtless admire His wisdom in working the spiritual world around the Wes Arabie 504-858-2881 physical one (cf. Isaiah 55:8–9; Romans 11:33). Howard Goodale 504-319-3572 Steve Tucker 504-812-2617 In this case, remarkably, four of God’s most important resources—Paul, Silas, Luke, and Timothy—were allocated to the task of winning one woman a EVANGELISTS continent away. Scott Cain 504-392-4110 Wes Arabie 504-858-2881 GOD’S PROVIDENCE IS SEEN IN CLOSED DOORS. -
Survey of the New Testament Is a Course Designed for Students with Little Knowledge in New Testament History and Background
Village Missions Ch. Website: www.vmcdi.com Contenders Discipleship Initiative E-mail: [email protected] New Testament Village Missions Matthew Contenders Discipleship Initiative Mark Luke Synoptics/Parables John Survey of the New Archaeology Acts & Epistles Testament Paul Revelation Local Instructor Guide Contenders Discipleship Initiative – New Testament Survey – Local Instructor Guide TRAINING MODULE SUMMARY Course Name New Testament Course Number in Series 3 Creation Date March 2016 Created By: Vernal Wilkinson Last Date Modified April 2017 Version Number 3.0 Copyright note: The Contenders program is provided free of charge and it is expected that those who receive freely will in turn give freely. Permission for non-commercial use is hereby granted but re-sale is prohibited. Contenders Bible School was a tuition-free two-year ministry equipping program started in 1995 by Pastor Ron Sallee at Machias Community Church, Snohomish, WA. It is now run as a tuition-free online equipping ministry by Village Missions. The full Contenders Discipleship Initiative program with pdf copies of this guide and corresponding videos can be found at www.vmcontenders.org. Copyright is retained by Village Missions with all rights reserved to protect the integrity of this material and the Village Missions Contenders Discipleship Initiative. Contenders Discipleship Initiative Disclaimer The views and opinions expressed in the Contenders Discipleship Initiative courses are those of the instructors and authors and do not necessarily reflect the official position of Village Missions. The viewpoints of Village Missions may be found at www.villagemissions.org/doctrinal-statement Village Missions may be found at www.villagemissions.org/doctrinal-statement LOCAL INSTRUCTORS using the CDI in a classroom setting are encouraged to fill in any gaps and add to the discussion of content provided by our ONLINE INSTRUCTORS. -
Acts V2 Master Edit
acts a commentary Volume 2 (acts 15-28) by dr doug mcintosh lilburn, georgia 170 Copyright © 2015 P. Douglas McIntosh. This data file/manuscript is the sole property of the copy- right holder and may be copied only in its entirety for circulation freely without charge. All copies of this data file/manuscript must contain the above copyright notice. This data file/manuscript may not be copied in part (except for small quotations used with citation of source), edited, revised, copied for resale, or incorporated in any commercial publications, recordings, broadcasts, performances, displays, or other products offered for sale without the written permission of the copyright holder. Requests for permission should be made in writing and addressed to Dr. Doug McIntosh, Senior Pastor, Cornerstone Bible Church, 869 Cole Drive, Lilburn, GA 30047. 171 172 introduction to the second half of acts Why did Luke write the Acts? What sources did he use in his composition? The Zondervan Ency- clopedia of the Bible supplies answers to these questions: “(1) Major portions of the second half of the book are the report of an eyewitness and a partici- pant in events. The account of the visit to Philippi in Acts 16 and that of the two voyages in chs. 20–21 and 27–28 read like a personal diary. The use of the first-person pronoun claims this au- thenticity, and the style and detail of the narrative in no way impugn the claim. (2) Events between Acts 21 and 27, where no personal participation is implied or claimed, never- theless took place while Luke was in contact with the situation, and these are recounted with a detail and a sureness of touch that mark them either as the work of an eyewitness or one in im- mediate contact with reliable witnesses. -
Slaves and Early Christianity Serving God Rather Than Human Masters
Post Augustum 2 (2018) Άρθρα Slaves and early Christianity Serving God rather than human masters Dimitris J. Kyrtatas Professor of Ancient History University of Thessaly Περίληψη: Το άπθπο αςηό αζσολείηαι με ηη θέζη ηυν δούλυν ζηιρ ππώηερ σπιζηιανικέρ κοινόηηηερ και ηιρ ανηιλήτειρ ηυν ππώηυν σπιζηιανών γύπυ από ηον θεζμό ηηρ δοςλείαρ. Πποζκομίζει μαπηςπίερ πος δείσνοςν όηι μολονόηι οι σπιζηιανοί δεν επέδειξαν ιδιαίηεπο ζήλο ζηον πποζηλςηιζμό ηυν δούλυν και δεν ςποζηήπιξαν ηη σειπαθέηηζή ηοςρ, ςπήπσαν απκεηοί δούλοι ζηιρ ππώηερ σπιζηιανικέρ κοινόηηηερ πος αθοζιώθηκαν ζηη νέα ηοςρ θπηζκεία, αποδεσόμενοι ακόμα και ηον μαπηςπικό θάναηο ζε πεπιόδοςρ διυγμών. Summary: This paper deals with the position of slaves within the early Christian communities and the views of the early Christians in respect to slavery as an institution. The testimonies produced suggest that although Christians did not give any priority to the conversion of slaves and did not promote their emancipation, there were several slave in the early Christian communities devoted to their new religion, that went as far as accepting martyrdom. Λέξεις κλειδιά: δούλοι, δοςλεία, σειπαθέηηζη, μαπηύπιο Key words: slaves, slavery, emancipation, martyrdom The early Christians were not happy with the distributing alms became an obligation to them, an world they lived in. Besides rejecting the religious essential part of their religious duties. beliefs and practices of their contemporaries, they Nevertheless, although surrounded by slaves, they were critical about the predominant morals and were never particularly concerned with slavery as their social consequences, and about the use of an institution, and never felt obliged to condemn it wealth and the way power was exercised. -
Revelation 2:18-29 the Church at Thyatira Chavurah Shalom Friday 11/18/16
Revelation 2:18-29 The Church at Thyatira Chavurah Shalom Friday 11/18/16 Our text begins, as each of the letters, with a statement of the author of the letter: Thus says The Son of God, who has eyes like a flame of fire, and His feet are like burnished bronze. The word "burnished" basically means that which is highly pol- ished, and shiny. We might say "reflective," and thus radiating the brilliance of the light shining out from Him. Thyatira (modern Ahisar) was located about thirty-five miles inland, between Pergamon and Sardis in northern Lydia, or Mysia (Strabo 13.4). According to le- gend, Thyatira was first established as a shrine to the sun god Tyrimnus and named Pelopia. ... Lydia of Thyatira, who lived in Philippi, was Paul’s first Christian convert in Europe (Acts 16:14–15, 40) and sold purple goods as a trade she had probably learned in Thyatira, which was particularly known for its dye- ing process and had a strong guild of dyers (Magie, Roman Rule 1:48; 2:812 n. 80)....One of the significant characteristics of Thyatira was the prominence of various trade guilds, including associations of clothiers, bakers, tanners, potters, linen workers, wool merchants, slave traders, shoemakers, dyers, and copper smiths (Magie, Roman Rule 1:48; 2:812 n. 78; A. H. M. Jones, Cities, 83; Hemer, BurH 11 [1975] 110).--David E. Aune, Word Biblical Commentary, p. 201. Some commentators suggest a specific contrast of "Son of God" with Zeus's son Apollo, a patron deity of Thyatira, with whom the deified emperors were linked.