DOCSLIB.ORG

Development and Characterization of SSR Markers for Trichloris Crinita Using Sequence Data from Related Grass Species

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Development and Characterization of SSR Markers for Trichloris Crinita Using Sequence Data from Related Grass Species SSRRev. FCAmarkers UNCUYO for. Trichloris2018. 50(1): crinita 1-16. ISSN impreso 0370-4661. ISSN (en línea) 1853-8665. Development and characterization of SSR markers for Trichloris crinita using sequence data from related grass species Desarrollo y caracterización de marcadores moleculares SSR para Trichloris crinita usando secuencias de gramíneas filogenéticamente cercanas Perla Carolina Kozub 1, Karina Barboza 2, Juan Bruno Cavagnaro 1, Pablo Federico Cavagnaro 2, 3 Originales: Recepción: 04/12/2017 - Aceptación: 07/05/2018 Abstract Trichloris crinita is among the most important native forage grasses in arid regions of America. Despite its importance, molecular resources and sequence data are extremely scarce in this species. In the present study, SSR markers were developed using available DNA sequences from grass taxa phylogenetically-related to Trichloris (Eleusine coracana, Cynodon dactylon and ‘Cynodon dactylon x Cynodon transvaalensis’). Marker transferability was evaluated in a panel of eight T. crinita of expected size in T. crinita, whereas transferability to other grass speciesaccessions ranged and from five 12closely-related (in Chloris castilloniana species. Of )the to 28105 SSRs SSR (in primer Eleusine pairs coracana evaluated,). Six 16 of theamplified 16 SSR products markers successfully transferred to T. crinita (37.5%) were polymorphic, and were further used to assess genetic diversity in eight T. crinita accessions. The analysis revealed a total of 23 SSR alleles (3.83 alleles/locus), allowing the discrimination of all T. crinita accessions, (and range) values for observed (Ho) and expected heterozygosity (He) were 0.53 (0.0-1.0)with pair-wise and 0.63 genetic (0.48-0.79), similarities respectively. ranging from 0.35 to 0.81 (Jaccard coefficient). Mean Keywords Chloridoideae • forage grass • genetic diversity • marker transferability • microsatellites 1 Universidad Nacional de Cuyo (UNCuyo). Facultad de Ciencias Agrarias (FCA). Instituto de Biología Agrícola de Mendoza (IBAM). Consejo Nacional de de Coria. Mendoza M5528AHB. Argentina. Investigaciones Científicas y Técnicas (CONICET). Almirante Brown 500. Chacras 2 40Consejo Km 96. Nacional San Carlos. de Investigaciones Mendoza 5567. Científicas Argentina. y [email protected]écnicas (CONICET); and 3 UniversidadInstituto Nacional Nacional de deTecnología Cuyo. Facultad Agropecuaria de Ciencias (INTA) Agrarias. E.E.A. Instituto La Consulta. de Horticultura. Ex ruta Tomo 50 • N° 1 • 2018 1 P. C. Kozub et al. Resumen Trichloris crinita es una importante gramínea forrajera, nativa de regiones áridas del continente americano. A pesar de su importancia, no existen herramientas moleculares ni marcadores moleculares SSR (“simple sequence repeats”) a partir de secuencias nucleo- secuencias nucleotídicas disponibles para esta especie.Trichloris En este (Eleusine estudio, coracana se desarrollaron, Cynodon dactylon y ‘Cynodon dactylon x Cynodon transvaalensis’) y se evaluó su transferibilidad entídicas ocho de accesiones especies filogenéticamentede T. crinita y cinco cercanas especies a de gramíneas cercanamente emparen- esperado en T. crinita, mientras que la transferibilidad a otras especies varió entre 12 (entadas. Chloris De los castilloniana 105 pares de) y cebadores28 SSRs (en evaluados, Eleusine coracana16 amplificaron). De los productos 16 SSRs transferibles del tamaño a T. crinita, seis . - tieron diferenciarfueron todas polimórficos las accesiones y se de utilizaron T. crinita, para analizar el grado de diversidad entregenética pares en ocho de accesiones accesiones de de 0,35esta especiea 0,81 (Jaccard).El análisis Se reveló obtuvieron 23 alelos, valores los cuales medios permi de heterocigosidad observada y esperada de 0,53 y 0,63, conrespectivamente. valores de similitud genética Palabras clave Chloridoideae • gramínea forrajera • diversidad genética • transferibilidad • microsatélites Introduction Trichloris crinita (Chloridoideae, phenotypes from natural populations, Poaceae) is one of the most important followed by their characterization based native grass species in arid regions of South on traits of interest, such as biomass America, due to its extensive area of distri- production (5), forage quality (7, 27), and bution (25), good forage quality (7, 27), drought resistance (12). Data from a recent and resistance to drought (13), tram- study (16), demonstrating that T. crinita is a pling and grazing by wild and domestic sexually-propagated autogamous species, animals (4). These characteristics and encourages the practice of conventional its competing aggressiveness among breeding strategies in the species. Along other native grasses (22), have led to with this, the possibility of developing a widespread utilization of T. crinita in molecular markers for assisting selection range grazing and revegetation projects in may accelerate T. crinita breeding goals. arid environments (24). Molecular resources in T. crinita are Despite its importance, advances in very scarce. To date, only two studies genetic research and breeding of T. crinita regarding the use of molecular markers have been limited, mainly due to the in this species have been published scarcity of molecular resources and the fact that the species mode of reproduction was unknown until very recently. The genetic(5, 16). Thediversity first study,in a T. publishedcrinita germplasm in 2006, main approach used to improve T. crinita collectionused AFLP (4), markers whereas for a characterizingmore recent has been the selection of interesting study by Kozub et al. (2017) used simple 2 Revista de la Facultad de Ciencias Agrarias SSR markers for Trichloris crinita sequence repeats (SSR) markers for Objectives inferring about the mode of reproduction of this species. The present work reports 1) To develop SSR markers for on the development and evaluation, at Trichloris crinita using sequence data various levels, of the SSR markers used in from Eleusine and Cynodon species. the latter report. 2) To evaluate marker transferability SSRs are robust and informative across T. crinita-related taxa. PCR-based markers and they are generally 3) To assess SSR polymorphism and genetic diversity in T. crinita accessions. level of polymorphism, reproducibility andfavored codominant over AFLPs dueinheritance to their higher(18). These markers have been successfully Materials and methods used for multiple genetic and breeding purposes in many crop species, including Source of sequence data, grapevine (19), carrot (6), alfalfa (12) and microsatellites search and primer design soybean (3). However, the development All the available genomic of SSR markers generally requires DNA sequences (GSS) of Eleusine coracana sequence data to search and detect SSR Cynodon dactylon (633 sequences; 0.4 Mbp), SSR. In the case of T. crinita, using sequence and ‘Cynodon dactylon x Cynodon databasesmotifs, and to design identify primers SSRs is flankingnot feasible the transvaalensis (404 sequences; 0.2 Mbp) since very few sequences are available for were downloaded from the NCBI database ’ (92 sequences; 0.04 Mbp) the species (only 34 T. crinita sequences on April 15, 2016. These species were are available at the NCBI database). selected based on their phylogenetic The use of sequence data from related proximity to T. crinita and the availability taxa for developing SSR markers in a of sequences in the database. species lacking sequence information has been widely used in plants, with program MISA (31). Only perfect micro- variable degrees of success, generally satellites,SSR motifs with awere basic identified motif of 2-6 using nt, andthe increasing success rate with the phylo- a minimum length of 12 nt (for di-, tri-, genetic proximity between SSR donor and tetranucleotides), 15 nt (for pentanu- and target species (15). This approach cleotides), and 18 nt (for hexanucleotides) has been particularly successful in the were considered. Poaceae family, where SSR markers were The position of the detected SSRs in developed for numerous orphan species, the genomic sequence was recorded, and using sequences from economically important cereal crops (34, 35). This designed. These SSR loci were selected strategy may also be effective for becauseprimers theypairs had flanking larger number105 SSRs of repeat were developing SSR markers for T. crinita units, as this feature has been associated using the available sequence data from with higher polymorphism rate (6). phylogenetically related genera, such as Eleusine and Cynodon. Tomo 50 • N° 1 • 2018 3 P. C. Kozub et al. For primer design, the software Primer3 v.4.0.0 (32) was used, using parameters to generate amplicons dNTPs (2.5 mM each), 1 μl 5μM of each of 200-500 bp, and primer length of wereprimer, programmed 0.3 μl Taq aspolymerase follows: initial at 3 dena U/μl- 22-30 nt with Tm of 55-62°C and GC turationand 2.5 μl at of 94°C genomic for 30DNA. sec, Thermocyclers followed by content of 30-50 %. Other parameters 40 cycles of 94°C for 45 sec, appropriate used the program default values. annealing temperature for 30 sec, and 72°C Plant materials Agarose (3%) gel electrophoresis used Trichloris crinita accessions for 1 min; and a final step of 72°C for 4 min. for visualization of the amplicons. A (CynodonEight dactylon, Eleusine coracana, 100ethidium bp ladder bromide (Invitrogen) (4 ul/100 ml wasof TAE used buffer) as Eleusineand five indica, related Chloris grass gayana species, and size marker. Denaturing polyacrylamide Chloris castilloniana) from the Germplasm (6%) gel electrophoresis
Load more

Recommended publications
  • Improved Conservation Plant Materials Released by NRCS and Cooperators Through December 2014
    Natural Resources Conservation Service Improved Conservation Plant Materials Released by Plant Materials Program NRCS and Cooperators through December 2014 Page intentionally left blank. Natural Resources Conservation Service Plant Materials Program Improved Conservation Plant Materials Released by NRCS and Cooperators Through December 2014 Norman A. Berg Plant Materials Center 8791 Beaver Dam Road Building 509, BARC-East Beltsville, Maryland 20705 U.S.A. Phone: (301) 504-8175 prepared by: Julie A. DePue Data Manager/Secretary [email protected] John M. Englert Plant Materials Program Leader [email protected] January 2015 Visit our Website: http://Plant-Materials.nrcs.usda.gov TABLE OF CONTENTS Topics Page Introduction ...........................................................................................................................................................1 Types of Plant Materials Releases ........................................................................................................................2 Sources of Plant Materials ....................................................................................................................................3 NRCS Conservation Plants Released in 2013 and 2014 .......................................................................................4 Complete Listing of Conservation Plants Released through December 2014 ......................................................6 Grasses ......................................................................................................................................................8
    [Show full text]
  • Cattle-Breeding Valley Plains and C4 Spontaneous Grasses in North Patagonia
    Journal of Environmental Science and Engineering A9 (2020) 266-272 doi:10.17265/2162-5298/2020.06.006 D DAVID PUBLISHING Cattle-Breeding Valley Plains and C4 Spontaneous Grasses in North Patagonia M. Guadalupe Klich Universidad Nacional de Río Negro, Escuela de Veterinaria y Producción Agroindustrial, CIT-UNRN-CONICET, Choele Choel 8360, Argentina Abstract: The climate of North Patagonia (Argentina) is semiarid and the region periodically suffers severe droughts that may last several years, decreasing forage offer and consequently cow livestock and productivity. In most of the cattle fields extensive grazing is usually continuous through the year-long. The absence of pasture rotational schemata affects rangeland health changing the composition of plants communities in detriment of the valuable species. When under a severe drought, the appreciated forage Leptochloa crinita (= Trichloris crinita) stopped reproduction and the population became scarce, a grazing schedule was designed in a cattle farm to avoid foraging during spring and summer in a paddock located in the valley plains, where the species was disappearing. Besides L. crinita populations, the sympatric presence of the Poaceae Aristida mendocina, Distichlis spicata and Distichlis scoparia is expected, each one in slightly different patches within the same area. The forage value differs between species but all of them are eaten by bovines. For ten years the plant communities were studied with the aims of determining the incidence of the patches on the paddock carrying capacity in early autumn and estimating the contribution of the four C4 species to bovine diet by microhistology. Free of grazing during its growing period, L. crinite enhanced the area of its patches and the biomass production of its good quality forage and was consumed preferently.
    [Show full text]
  • Plant Ecology of Arid-Land Wetlands; a Watershed Moment for Ciénega Conservation
    Plant Ecology of Arid-land Wetlands; a Watershed Moment for Ciénega Conservation by Dustin Wolkis A Thesis Presented in Partial Fulfillment of the Requirements for the Degree Master of Science Approved February 2016 by the Graduate Supervisory Committee: Juliet C Stromberg, Chair Sharon Hall Andrew Salywon Elizabeth Makings ARIZONA STATE UNIVERSITY May 2016 ABSTRACT It’s no secret that wetlands have dramatically declined in the arid and semiarid American West, yet the small number of wetlands that persist provide vital ecosystem services. Ciénega is a term that refers to a freshwater arid-land wetland. Today, even in areas where ciénegas are prominent they occupy less than 0.1% of the landscape. This investigation assesses the distribution of vascular plant species within and among ciénegas and address linkages between environmental factors and wetland plant communities. Specifically, I ask: 1) What is the range of variability among ciénegas, with respect to wetland area, soil organic matter, plant species richness, and species composition? 2) How is plant species richness influenced locally by soil moisture, soil salinity, and canopy cover, and regionally by elevation, flow gradient (percent slope), and temporally by season? And 3) Within ciénegas, how do soil moisture, soil salinity, and canopy cover influence plant species community composition? To answer these questions I measured environmental variables and quantified vegetation at six cienegas within the Santa Cruz Watershed in southern Arizona over one spring and two post-monsoon periods. Ciénegas are highly variable with respect to wetland area, soil organic matter, plant species richness, and species composition. Therefore, it is important to conserve the ciénega landscape as opposed to conserving a single ciénega.
    [Show full text]
  • Low Water Use Drought Tolerant Plant List
    LowLowLowLow WaterWaterWaterWater UseUseUseUse DroughtDroughtDroughtDrought TolerantTolerantTolerantTolerant PlantPlantPlantPlant ListListListList OfficialOfficial RegulatoryRegulatory ListList forfor thethe ArizonaArizona DepartmentDepartment ofof WaterWater Resources,Resources, TucsonTucson ActiveActive ManagementManagement AreaArea 400400 W.W. Congress,Congress, SuiteSuite 518518 (520)(520) 770-3800 770-3800 Tucson,Tucson, AZAZ 8570185701 www.azwater.govwww.azwater.gov Photo - Christina Bickelmann 2004 D ROP YOUR WATER USE!! Tucson is Educating Consumers to Drop their Water Use . The Tucson office of the Arizona Department of Water Resources (ADWR) has developed a new pilot program, Drop Your Water Use, to educate retail nursery customers on how to plan and maintain a healthy and water efficient landscape. The program is a voluntary collaboration between ADWR and local nurseries that uses a simple water drop system to identify plants with similar water requirements. Plants are labeled one through three, distinguished with corresponding water drops and numbers. All of the labeled plants are Xeriscape plants currently on the Tucson AMA -Low Water Use/ Drought Tolerant plant list. The one through three numbering system correlates with the plant list. A “number one” indicates very low water use mostly native Sonoran and Chihuahuan desert plants, Two is low water use, Three is moderate water use. By choosing plants with the same number a gardener can more successfully group plants by water needs. Although the plants are all low-water-using, the frequency that they need to be irrigated varies from approximately once a week to once a month after the plants are established (in about 2-3 years). ADWR hopes that consumers will use separate valves on their irrigation system to water each zone and irrigate trees separately, enabling them to manage their irrigation water use more efficiently.
    [Show full text]
  • SELECCIÓN ENTRE Panicum Urvilleanum, Pappophorum Caespitosum, Y CUATRO GENOTIPOS DE Leptochloa Crinita, BAJO SALINIDAD Y SEQUÍA, CON PERSPECTIVA DE REVEGETACIÓN
    SELECCIÓN ENTRE Panicum urvilleanum, Pappophorum caespitosum, Y CUATRO GENOTIPOS DE Leptochloa crinita, BAJO SALINIDAD Y SEQUÍA, CON PERSPECTIVA DE REVEGETACIÓN. TESIS DE GRADO INGENIERÍA EN RECURSOS NATURALES RENOVABLES 2020 Tesista: Yanina Cristina, Buccolini Lucas Directora: Sartor, Carmen Elena Codirectora: Kozub, Perla Carolina Facultad de Ciencias Agrarias Universidad Nacional de Cuyo Almirante Brown 500, Chacras de Coria, Mendoza M5528AHB- (54 261) 4135000. SELECCIÓN ENTRE Panicum urvilleanum, Pappophorum caespitosum, Y CUATRO GENOTIPOS DE Leptochloa crinita, BAJO SALINIDAD Y SEQUÍA, CON PERSPECTIVA DE REVEGETACIÓN. Tesista: Yanina Cristina, Buccolini Lucas [email protected] Directora: Dra. Sartor, Carmen Elena [email protected] Codirectora: Dra. Kozub, Perla Carolina [email protected] Jurado: Mg. Ing. Greco, Silvina Alicia [email protected] Dra. Fernández, María Emilia [email protected] Ing. Domínguez, Deolindo Luis Esteban [email protected] ii RESUMEN: La desertificación es una de las preocupaciones ambientales más relevantes en la Argentina, con el 75% del territorio bajo condiciones áridas y semiáridas. Es por ello que las prácticas productivas en estas zonas áridas y semiáridas, se desarrollan en oasis bajo riego, en los cuales el 40% de su superficie presenta problemas de degradación, provocados en gran medida por la salinización. En este contexto, los oasis mendocinos utilizan íntegramente los caudales de los ríos, mientras que las áreas deprimidas del desierto no reciben aportes hídricos superficiales. La vegetación evidencia estos impactos, y es ella quien brinda recursos fundamentales para la recuperación de estos ambientes. Siendo la revegetación con especies nativas, una de las herramientas más promisorias para la restauración de estos ambientes sensibles y de baja recuperación natural.
    [Show full text]
  • Flora of Southwestern Arizona
    Felger, R.S., S. Rutman, and J. Malusa. 2014. Ajo Peak to Tinajas Altas: A flora of southwestern Arizona. Part 6. Poaceae – grass family. Phytoneuron 2014-35: 1–139. Published 17 March 2014. ISSN 2153 733X AJO PEAK TO TINAJAS ALTAS: A FLORA OF SOUTHWESTERN ARIZONA Part 6. POACEAE – GRASS FAMILY RICHARD STEPHEN FELGER Herbarium, University of Arizona Tucson, Arizona 85721 & Sky Island Alliance P.O. Box 41165, Tucson, Arizona 85717 *Author for correspondence: [email protected] SUSAN RUTMAN 90 West 10th Street Ajo, Arizona 85321 JIM MALUSA School of Natural Resources and the Environment University of Arizona Tucson, Arizona 85721 [email protected] ABSTRACT A floristic account is provided for the grass family as part of the vascular plant flora of the contiguous protected areas of Organ Pipe Cactus National Monument, Cabeza Prieta National Wildlife Refuge, and the Tinajas Altas Region in southwestern Arizona. This is the second largest family in the flora area after Asteraceae. A total of 97 taxa in 46 genera of grasses are included in this publication, which includes ones established and reproducing in the modern flora (86 taxa in 43 genera), some occurring at the margins of the flora area or no long known from the area, and ice age fossils. At least 28 taxa are known by fossils recovered from packrat middens, five of which have not been found in the modern flora: little barley ( Hordeum pusillum ), cliff muhly ( Muhlenbergia polycaulis ), Paspalum sp., mutton bluegrass ( Poa fendleriana ), and bulb panic grass ( Zuloagaea bulbosa ). Non-native grasses are represented by 27 species, or 28% of the modern grass flora.
    [Show full text]
  • Development and Characterization of SSR Markers for Trichloris Crinita Using Sequence Data from Related Grass Species
    SSRRev. FCAmarkers UNCUYO for. Trichloris2018. 50(1): crinita 1-16. ISSN impreso 0370-4661. ISSN (en línea) 1853-8665. Development and characterization of SSR markers for Trichloris crinita using sequence data from related grass species Desarrollo y caracterización de marcadores moleculares SSR para Trichloris crinita usando secuencias de gramíneas filogenéticamente cercanas Perla Carolina Kozub 1, Karina Barboza 2, Juan Bruno Cavagnaro 1, Pablo Federico Cavagnaro 2, 3 Originales: Recepción: 04/12/2017 - Aceptación: 07/05/2018 Abstract Trichloris crinita is among the most important native forage grasses in arid regions of America. Despite its importance, molecular resources and sequence data are extremely scarce in this species. In the present study, SSR markers were developed using available DNA sequences from grass taxa phylogenetically-related to Trichloris (Eleusine coracana, Cynodon dactylon and ‘Cynodon dactylon x Cynodon transvaalensis’). Marker transferability was evaluated in a panel of eight T. crinita of expected size in T. crinita, whereas transferability to other grass speciesaccessions ranged and from five 12closely-related (in Chloris castilloniana species. Of )the to 28105 SSRs SSR (in primer Eleusine pairs coracana evaluated,). Six 16 of theamplified 16 SSR products markers successfully transferred to T. crinita (37.5%) were polymorphic, and were further used to assess genetic diversity in eight T. crinita accessions. The analysis revealed a total of 23 SSR alleles (3.83 alleles/locus), allowing the discrimination of all T. crinita accessions, (and range) values for observed (Ho) and expected heterozygosity (He) were 0.53 (0.0-1.0)with pair-wise and 0.63 genetic (0.48-0.79), similarities respectively.
    [Show full text]
  • Ecoregions of Texas
    ECOREGIONS OF TEXAS Glenn Griffith, Sandy Bryce, James Omernik, and Anne Rogers ECOREGIONS OF TEXAS Glenn Griffith1, Sandy Bryce2, James Omernik3, and Anne Rogers4 December 27, 2007 1Dynamac Corporation 200 SW 35th Street, Corvallis, OR 97333 (541) 754-4465; email: [email protected] 2Dynamac Corporation 200 SW 35th Street, Corvallis, OR 97333 (541) 754-4788; email: [email protected] 3U.S. Geological Survey c/o U.S. Environmental Protection Agency National Health and Environmental Effects Research Laboratory 200 SW 35th Street, Corvallis, OR 97333 (541) 754-4458; email: [email protected] 4Texas Commission on Environmental Quality Surface Water Quality Monitoring Program 12100 Park 35 Circle, Building B, Austin, TX 78753 (512) 239-4597; email [email protected] Project report to Texas Commission on Environmental Quality The preparation of this report and map was financed in part by funds from the U.S. Environmental Protection Agency Region VI, Regional Applied Research Effort (RARE) and Total Maximum Daily Load (TMDL) programs. ABSTRACT Ecoregions denote areas of general similarity in ecosystems and in the type, quality, and quantity of environmental resources. Ecoregion frameworks are valuable tools for environmental research, assessment, management, and monitoring of ecosystems and ecosystem components. They have been used for setting resource management goals, developing biological criteria and establishing water quality standards. In a cooperative project with the Texas Commission on Environmental Quality, the U.S. Environmental Protection Agency, the U.S. Department of Agriculture, and other interested state and federal agencies, we have defined ecological regions of Texas at two hierarchical levels that are consistent and compatible with the U.S.
    [Show full text]
  • Cross-Species Transfer of SSR Markers in Setaria Sphacelata and Trichloris Crinita Sp
    Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp. Transferencia cruzada de marcadores SSR en Setaria sphacelata y Trichloris crinita sp. Cecilia Paola Randazzo1*, Andrea Matilde Ferri1, Leonela Carabajal Paladino2, Adriana Noemi Andres3, and Lorena Romina Ingala1 ABSTRACT RESUMEN Setaria sphacelata and Trichloris crinita are subtropical Setaria sphacelata y Trichloris crinita son especies forrajeras forage species that are important for livestock breeding in subtropicales, estratégicas para el desarrollo de la actividad ga- Argentina. Genomic information is scarce for these species, nadera argentina. Para estas especies, la información genómica and there are no molecular markers designed for them; this es escasa y no existen marcadores moleculares desarrollados limits the development of genetic improvement programs. en las mismas, por lo cual el desarrollo de programas de mejo- We performed a cross-species transfer of SSR markers from ramiento genético se ve limitado. En este contexto, realizamos several Poaceae species. In S. sphacelata, 8 SSR markers were una transferencia de marcadores SSR de varias especies de transferred from Setaria italica (40% transfer rate), exhibiting poáceas. En S. sphacelata, se transfirieron 8 marcadores de- 83% polymorphism. Kazungula, Splenda and Narok cultivars sarrollados en Setaria italica (tasa de transferencia del 40%), were genetically differentiated and the experimental material mostrando un 83% de polimorfismo. Los cultivares Kazungula, “Selección INTA” was separated from Narok, from which it was Splenda y Narok se diferenciaron genéticamente y el material derived. For T. crinita, 19 microsatellites were transferred from experimental “Selección INTA” se separó de Narok, del cual 5 Poaceae species (7.3% transfer rate), with 69% polymorphism.
    [Show full text]
  • Reproductive Biology of the Native Forage Grass Trichloris Crinita
    Received Date : 30-Sep-2016 Revised Date : 07-Dec-2016 Accepted Date : 25-Jan-2017 Article type : Research Paper Editor : J.Arroyo Reproductive biology of the native forage grass Trichloris crinita (Poaceae, Chloridoideae) Perla Carolina Kozub1, Karina Barboza2, Florencia Galdeano3, Camilo Luis Quarin3, Juan Bruno Cavagnaro1, Pablo Federico Cavagnaro2, 4* Article 1 Instituto de Biología Agrícola de Mendoza (IBAM), Facultad de Ciencias Agrarias (FCA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Cuyo (UNCuyo), Mendoza, Argentina. 2 Instituto Nacional de Tecnología Agropecuaria (INTA) E.E.A. La Consulta; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza, Argentina. 3 Instituto de Botánica del Nordeste (IBONE), Facultad de Ciencias Agrarias (FCA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional del Nordeste (UNNE), Corrientes, Argentina. 4 Instituto de Horticultura, Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, Argentina. * Corresponding author: [email protected] This article has been accepted for publication and undergone full peer review but has not Accepted been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1111/plb.12549 This article is protected by copyright. All rights reserved. Abstract Trichloris crinita is a perennial forage grass species native to arid regions of the American continent. Due to its extensive area of distribution, good forage quality, and resistance to drought and grazing, this species is widely utilized as forage and for revegetation purposes in environments with low water availability. Despite its importance, genetic improvement of T.
    [Show full text]
  • Chloridoideae Paul M
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Scholarship@Claremont Aliso: A Journal of Systematic and Evolutionary Botany Volume 23 | Issue 1 Article 43 2007 Classification and Biogeography of New World Grasses: Chloridoideae Paul M. Peterson National Museum of Natural History, Smithsonian Institution, Washington, D.C. J. Travis Columbus Rancho Santa Ana Botanic Garden, Claremont, California Susan J. Pennington National Museum of Natural History, Smithsonian Institution, Washington, D.C. Follow this and additional works at: http://scholarship.claremont.edu/aliso Part of the Botany Commons, and the Ecology and Evolutionary Biology Commons Recommended Citation Peterson, Paul M.; Columbus, J. Travis; and Pennington, Susan J. (2007) "Classification and Biogeography of New World Grasses: Chloridoideae," Aliso: A Journal of Systematic and Evolutionary Botany: Vol. 23: Iss. 1, Article 43. Available at: http://scholarship.claremont.edu/aliso/vol23/iss1/43 Aliso 23, pp. 580–594 ᭧ 2007, Rancho Santa Ana Botanic Garden CLASSIFICATION AND BIOGEOGRAPHY OF NEW WORLD GRASSES: CHLORIDOIDEAE PAUL M. PETERSON,1,3 J. TRAVIS COLUMBUS,2 AND SUSAN J. PENNINGTON1 1Department of Botany, National Museum of Natural History, Smithsonian Institution, Washington, D.C. 20013-7012, USA; 2Rancho Santa Ana Botanic Garden, 1500 North College Avenue, Claremont, California 91711-3157, USA 3Corresponding author ([email protected]) ABSTRACT Subfamily Chloridoideae (Poaceae) in the New World includes 72 genera (61 native, 11 introduced), 678 species (607 native), and, including intraspecific taxa, 817 total taxa. The five largest genera are Muhlenbergia (147 species), Eragrostis (111), Sporobolus (76), Bouteloua (57), and Chloris (35). Three tribes are recognized in this study: Cynodonteae, Eragrostideae, and Zoysieae, with ten, three, and two subtribes, respectively.
    [Show full text]
  • Environmental Assessment Supporting the Mechanical Control of Carrizo Cane in the Rio Grande Basin in Texas
    Draft Environmental Assessment Supporting the Mechanical Control of Carrizo Cane in the Rio Grande Basin in Texas Department of Homeland Security U.S. Customs and Border Protection July 2016 Abbreviations and Acronyms BMP best management practice NHPA National Historic Preservation CBP U.S. Customs and Border Act Protection NO2 nitrogen dioxide CEQ Council on Environmental NOx nitrogen oxide Quality NPS National Park Service CFR Code of Federal Regulations NRHP National Register of Historic CO carbon monoxide Places CWA Clean Water Act O3 ozone dBA A-weighted decibel P.L. Public Law DHS Department of Homeland Pb lead Security percent g percent of the force of gravity EA Environmental Assessment PM2.5 particulate matter less than or EIS Environmental Impact equal to 2.5 microns in diameter Statement PM10 particulate matter less than or EO Executive Order equal to 10 microns in diameter ESA Endangered Species Act RCRA Resources Conservation and ESP Environmental Stewardship Recovery Act Plan ROW right-of-way FEMA Federal Emergency SO2 sulfur dioxide Management Agency TCEQ Texas Commission on FM&E Facilities Management and Environmental Quality Engineering TPWD Texas Parks and Wildlife FONSI Finding of No Significant Department Impact tpy tons per year FPPA Farmland Protection Policy Act TXDOT Texas Department of FR Federal Register Transportation HUC hydrologic unit code USC United States Code mph miles per hour USACE U.S. Army Corps of Engineers msl mean sea level USBP U.S. Border Patrol NAAQS National Ambient Air Quality USEPA U.S. Environmental Protection Standards Agency NAGPRA Native American Graves USFWS U.S. Fish and Wildlife Service Protection and Repatriation Act VOC volatile organic compounds NEPA National Environmental Policy Act Cover Sheet Draft Environmental Assessment Supporting the Mechanical Control of Carrizo Cane in the Rio Grande Basin in Texas Responsible Agencies: Department of Homeland Security, U.S.
    [Show full text]