bioRxiv preprint doi: https://doi.org/10.1101/164681; this version posted July 17, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 ORIGINAL ARTICLE 2 DNA barcoding British Euphrasia reveals deeply divergent polyploids but lack of 3 species-level resolution 4 Xumei Wang1, Galina Gussarova2,3,4, Markus Ruhsam5, Natasha de Vere6, Chris Metherell7, 5 Peter M. Hollingsworth5, Alex D. Twyford8* 6 1 School of Pharmacy, Xi'an Jiaotong University, 76 Yanta West Road, Xi'an, 710061, 7 P.R.China 8 2 Tromsø University Museum, UiT The Arctic University of Norway, PO box 6050 Langnes, 9 NO-9037, Tromsø, Norway 10 3 CEES-Centre for Ecological and Evolutionary Synthesis, Dept. of Biosciences, University 11 of Oslo, PO BOX 1066, Blindern, NO-0316, Norway 12 4 Dept. of Botany, Faculty of Biology, St Petersburg State University, Universitetskaya nab. 13 7/9, 199034 St Petersburg, Russia 14 5 Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh, EH3 5LR, UK 15 6 National Botanic Garden of Wales, Llanarthne, Carmarthenshire, SA32 8HG, UK and 16 Institute of Biological, Environmental & Rural Sciences (IBERS), Aberystwyth University, 17 Penglais, Aberystwyth, Ceredigion, SY23 3DA, UK 18 7 57 Walton Rd, Bristol BS11 9TA 19 8 University of Edinburgh, Institute of Evolutionary Biology, West Mains Road, Edinburgh, 20 EH3 9JT, UK 21 22 Running title: Complex patterns of diversity in Euphrasia 23 For correspondence:
[email protected] 24 1 bioRxiv preprint doi: https://doi.org/10.1101/164681; this version posted July 17, 2017.