Recombinant VACV Fc Chimera (A.A

Recombinant VACV Fc Chimera (a.a. 17-258, 18- 258, 100-330) [His] (DAG2641) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Product Overview Recombinant Viral CCI Fc Chimera antigen, was expressed in Spodoptera frugiperda, Sf 21 (baculovirus). N-terminus Viral CCI (Met17-Val258) & (Pro18-Val258) (Accession # , P19063), C- terminus Human IgG1 (Pro100-Lys330) and 6-His tag, Disulfide-linked homod Nature Recombinant Expression System Insect cells Species VACV Purity > 90%, by SDS-PAGE under reducing conditions and visualized by silver stain. Conjugate His Procedure None Format Lyophilized from a 0.2 μm filtered solution in PBS Preservative None Storage 2-8°C short term, -20°C long term BACKGROUND Introduction Vaccinia virus is a large, complex, enveloped virus belonging to the poxvirus family. It has a linear, double-stranded DNA genome approximately 190 kbp in length, and which encodes for approximately 250 genes. The dimensions of the virion are roughly 360 × 270 × 250 nm, with a mass of approximately 5-10 fg. Vaccinia virus is well known for its role as a vaccine (its namesake) that eradicated the smallpox disease, making it the first human disease to be successfully eradicated by science. This endeavour was carried out by the World Health Organization under the Smallpox Eradication Program. Post eradication of smallpox, scientists study Vaccinia virus to use as a tool for delivering genes into biological tissues (gene therapy and genetic engineering). Keywords Vaccinia virus vCCI protein, Human IgG1 protein; Orthopoxvirus; Vaccinia virus; 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved Chordopoxvirinae; VACV; VV 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 2 © Creative Diagnostics All Rights Reserved.

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Patentability of Human-Animal Chimeras Ryan Hagglund
Santa Clara High Technology Law Journal Volume 25 | Issue 1 Article 4 2008 Patentability of Human-Animal Chimeras Ryan Hagglund Follow this and additional works at: http://digitalcommons.law.scu.edu/chtlj Part of the Law Commons Recommended Citation Ryan Hagglund, Patentability of Human-Animal Chimeras, 25 Santa Clara High Tech. L.J. 51 (2012). Available at: http://digitalcommons.law.scu.edu/chtlj/vol25/iss1/4 This Article is brought to you for free and open access by the Journals at Santa Clara Law Digital Commons. It has been accepted for inclusion in Santa Clara High Technology Law Journal by an authorized administrator of Santa Clara Law Digital Commons. For more information, please contact [email protected]. PATENTABILITY OF HUMAN-ANIMAL CHIMERAS Ryan Hagglundt Abstract The chimera was a mythological creature with a lion 's head, goat's body, and serpent's tail. Because of recent advances in biotechnology, such permutations on species are no longer the stuff of myth and legend The term "chimera " has come to describe a class of genetically engineered creatures composed of some cells from one species, which thus contain genetic material derived entirely from that species, and some cells from another species, containing only genetic material from that species. Scientists have created a goat- sheep chimera or "geep" which exhibits physical characteristicsof both animals. Likewise, scientists have also used the tools of modern molecular biology to create human-animal chimeras containing both human and animal cells. While none of the human-animal chimeras hitherto created have exhibited significant human characteristics,the synthesis of human-animal chimeras raises significant ethical concerns.
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Molecular Phylogenetic Analysis
Assignment #2 Physics 498: Statistical Physics of Biological Complexity and Information Molecular Phylogenetic Analysis Date of Submission: 31th October, 2001 Rahul Roy Center for Biophysics and Computational Biology University of Illinois at Urbana-Champaign email: [email protected] Evolve by Borrowing ? Rahul Roy Center for Biophysics and Computational Biology University of Illinois at Urbana Champaign Introduction Molecular evolution over a period of four billion years has resulted in the development of present myriad of species from a hot soup of molecules. It has resulted in the evolution of complex multicellular organisms (Eukaryotes) along with single-celled organisms like prokaryotes at the same time. The question that has baffled scientists for long is: how did this incredible transition from a soup to this complex state take place? The discussion, that still continues, as how life started from the primordial soup is not the emphasis of the present debate, though Stanley Miller showed in, as back as 1950s, that organic chemicals can be synthesized from completely inorganic inputs. It has been proposed based on structural and functional complexity and fossil evidence that prokaryotes must have predated the eukaryotes by at least 1.0-2.0 billion years [Knoll, 1992]. This has led to the notion that eukaryotic cells have evolved from more simple prokaryotic organisms with which they share numerous common (or related) molecules [Margulis, 1970; Zillig, 1991]. Indeed, studies have shown that a number of eukaryotic organelles (namely mitochondria and chloroplasts) bear a close evolutionary relationship to specific groups of prokaryotes (namely α-proteobacteria and cyanobacteria, respectively) [Gray, 1992] So, how did the complex multicellular eukaryotes evolve from prokaryotes? To understand the origin of the eukaryotic cells a number of theories have been proposed.
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BWPP Biological Weapons Reader
BWPP Biological Weapons Reader Edited by Kathryn McLaughlin and Kathryn Nixdorff Geneva 2009 About BWPP The BioWeapons Prevention Project (BWPP) is a global civil society activity that aims to strengthen the norm against using disease as a weapon. It was initiated by a group of non- governmental organizations concerned at the failure of governments to act. The BWPP tracks governmental and other behaviour that is pertinent to compliance with international treaties and other agreements, especially those that outlaw hostile use of biotechnology. The project works to reduce the threat of bioweapons by monitoring and reporting throughout the world. BWPP supports and is supported by a global network of partners. For more information see: http://www.bwpp.org Table of Contents Preface .................................................................................................................................................ii Abbreviations .....................................................................................................................................iii Chapter 1. An Introduction to Biological Weapons ......................................................................1 Malcolm R. Dando and Kathryn Nixdorff Chapter 2. History of BTW Disarmament...................................................................................13 Marie Isabelle Chevrier Chapter 3. The Biological Weapons Convention: Content, Review Process and Efforts to Strengthen the Convention.........................................................................................19
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Formation and Longevity of Chimeric and Duplicate Genes in Drosophila Melanogaster
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Infectious Enveloped RNA Virus Antigenic Chimeras (Alphaviruses/Random Mutagenesis/Virus Vectors/Protein Engineering/Glycoproteins) STEVEN D
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Chimeric Brains Consisted of Primatologists and Other Scientists, Ethicists, and Lawyers
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bioRxiv preprint doi: https://doi.org/10.1101/283127; this version posted March 15, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Long-read DNA metabarcoding of ribosomal rRNA in the analysis of fungi from aquatic environments Felix Heeger1,2†, Elizabeth C. Bourne1,2†, Christiane Baschien3, Andrey Yurkov3, Boyke Bunk3, Cathrin Spröer3, Jörg Overmann3, Camila J. Mazzoni2,4‡, Michael T. Monaghan1,2‡ 1Leibniz Institute of Freshwater Ecology and Inland Fisheries (IGB), Müggelseedamm 301, 12587 Berlin, Germany 2Berlin Center for Genomics in Biodiversity Research, Königin-Luise-Str. 6-8, 12489 Berlin, Germany 3Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7 B, 38124 Braunschweig, Germany 4Leibniz Institute of Zoo- and Wildlife Research (IZW), Alfred-Kowalke-Straße 17, 10315 Berlin, Germany † these authors contributed equally to this work ‡ these authors contributed equally to this work KEYWORDS: aquatic, biodiversity, CCS, chimera formation, eukaryotes, freshwater, fungi, isolates, long DNA barcodes, metabarcoding, mock community, Pacifc Biosciences, SMRT ABSTRACT DNA metabarcoding is now widely used to study prokaryotic and eukaryotic microbial diversity. Technological constraints have limited most studies to marker lengths of ca. 300-600 bp. Longer sequencing reads of several 5 thousand bp are now possible with third-generation sequencing. The increased marker lengths provide greater taxonomic resolution and enable the use of phylogenetic methods of classifcation, but longer reads may be subject to higher rates of sequencing error and chimera formation. In addition, most well- established bioinformatics tools for DNA metabarcoding were originally 10 designed for short reads and are therefore not suitable.
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Downloaded from genome.cshlp.org on September 26, 2021 - Published by Cold Spring Harbor Laboratory Press Resource Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons Brian J. Haas,1,9 Dirk Gevers,1 Ashlee M. Earl,1 Mike Feldgarden,1 Doyle V. Ward,1 Georgia Giannoukos,1 Dawn Ciulla,1 Diana Tabbaa,1 Sarah K. Highlander,2,3 Erica Sodergren,4 Barbara Methe´,5 Todd Z. DeSantis,6 The Human Microbiome Consortium, Joseph F. Petrosino,2,3 Rob Knight,7,8 and Bruce W. Birren1 1Genome Sequencing and Analysis Program, The Broad Institute, Cambridge, Massachusetts 02142, USA; 2Human Genome Sequencing Center, Baylor College of Medicine, Houston, Texas 77030, USA; 3Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030, USA; 4The Genome Center, Washington University School of Medicine, St. Louis, Missouri 63108, USA; 5Human Genomic Medicine, J. Craig Venter Institute, Rockville, Maryland 20850, USA; 6Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA; 7Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, USA; 8Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado 80309, USA Bacterial diversity among environmental samples is commonly assessed with PCR-amplified 16S rRNA gene (16S) se- quences. Perceived diversity, however, can be influenced by sample preparation, primer selection, and formation of chimeric 16S amplification products. Chimeras are hybrid products between multiple parent sequences that can be falsely interpreted as novel organisms, thus inflating apparent diversity. We developed a new chimera detection tool called Chimera Slayer (CS). CS detects chimeras with greater sensitivity than previous methods, performs well on short se- quences such as those produced by the 454 Life Sciences (Roche) Genome Sequencer, and can scale to large data sets.
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Detection of a New Resistance-Mediating Plasmid Chimera in a Blaoxa-48-Positive Klebsiella Pneumoniae Strain at a German University Hospital
microorganisms Article Detection of a New Resistance-Mediating Plasmid Chimera in a blaOXA-48-Positive Klebsiella pneumoniae Strain at a German University Hospital Julian Schwanbeck 1,† , Wolfgang Bohne 1,†, Ufuk Hasdemir 2 , Uwe Groß 1, Yvonne Pfeifer 3, Boyke Bunk 4, Thomas Riedel 4,5, Cathrin Spröer 4, Jörg Overmann 4,5, Hagen Frickmann 6,7,‡ and Andreas E. Zautner 1,*,‡ 1 Institute for Medical Microbiology, University Medical Center Göttingen, 37075 Göttingen, Germany; [email protected] (J.S.); [email protected] (W.B.); [email protected] (U.G.) 2 Department of Medical Microbiology, School of Medicine, Marmara University, 34854 Istanbul, Turkey; [email protected] 3 Robert Koch Institute, FG13 Nosocomial Infections and Antibiotic Resistance, 38855 Wernigerode, Germany; [email protected] 4 Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, Germany; [email protected] (B.B.); [email protected] (T.R.); [email protected] (C.S.); [email protected] (J.O.) 5 German Center for Infection Research (DZIF), Partner Site Hannover-Braunschweig, 38124 Braunschweig, Germany 6 Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, 20359 Hamburg, Germany; [email protected] Citation: Schwanbeck, J.; Bohne, W.; 7 Institute for Medical Microbiology, Virology and Hygiene, University Medicine Rostock, Hasdemir, U.; Groß, U.; Pfeifer, Y.; 18057 Rostock, Germany Bunk, B.; Riedel, T.; Spröer, C.; * Correspondence: [email protected]; Tel.: +49-551-39-65927 Overmann, J.; Frickmann, H.; et al. † These authors contributed equally to this work. Detection of a New ‡ These authors contributed equally to this work. Resistance-Mediating Plasmid Chimera in a blaOXA-48-Positive Abstract: Mobile genetic elements, such as plasmids, facilitate the spread of antibiotic resistance Klebsiella pneumoniae Strain at a genes in Enterobacterales.
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Human-Animal Chimeras
Issue Brief June 2021 | No. IF21F01 Human-Animal Chimeras: Unethical and Unnecessary by Mary Szoch, M.Ed. Key Points n the United States, the ethical considerations regarding Human-animal chimeras are human-animal chimeras and human embryo research are creatures that are part human and part animal. Since 1979, intertwined. Understanding the regulations on human- there has been a restriction, Ianimal chimeras the United States ought to have requires a known as the 14-Day Rule, on developing this type of review of the United States’ policies on human embryo creature past 14 days gestation research, an overview of what human-animal chimeras are, an in vitro. analysis of the purported benefits of human-animal chimera In 2016, after scientists gained the ability to develop an research, and a synopsis of the ethical questions posed by embryo in vitro beyond nine creating these creatures. days, the National Institutes of Health revisited the 14-Day Rule, but under the pro-life Trump administration, the Rule The serious ethical concerns about the creation of human- was kept in place. animal chimeras and the lack of any beneficial scientific After a partnership with China advancements from chimera experiments make it clear that created the first human- primate chimera, the NIH, human-animal chimeras are unethical and unnecessary. under the Biden administration, opted to once again review the 14-Day Rule. Senators recently voted along party lines against banning the creation of human- animal chimeras through legislation. This report can be read online at frc.org/chimeras 801 G. St. NW Washington, D.C.
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Construction of Intertypic Chimeric Dengue Viruses by Substitution Of
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