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Effects of Temperature and Tissue Type on the Development of Megaselia Scalaris (Diptera: Phoridae)

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Effects of Temperature and Tissue Type on the Development of Megaselia Scalaris (Diptera: Phoridae) Journal of Medical Entomology Advance Access published March 29, 2016 Journal of Medical Entomology, 2016, 1–7 doi: 10.1093/jme/tjw019 Development, Life History Research article Effects of Temperature and Tissue Type on the Development of Megaselia scalaris (Diptera: Phoridae) Joshua K. Thomas,1,2 Michelle R. Sanford,3 Michael Longnecker,4 and Jeffery K. Tomberlin1 1Department of Entomology, Texas A&M University, College Station, TX 77843 ([email protected]; [email protected]), 2Corresponding author, e-mail: [email protected], 3Harris County Institute for Forensic Science, Houston, TX 77030 ([email protected]) and 4Department of Statistics, 77843 Texas A&M University, College Station, TX ([email protected]) Received 5 January 2016; Accepted 18 February 2016 Downloaded from Abstract The scuttle fly, Megaselia scalaris (Loew) (Diptera: Phoridae), is of medical, veterinary, and forensic importance. In the case of the latter, M. scalaris is commonly associated with indoor death or neglect cases of humans or household animals, and its larvae are useful in determining time of colonization (TOC). This study is the first to examine the effects of different temperatures and tissues from two vertebrate species on the growth rate and http://jme.oxfordjournals.org/ larval length of M. scalaris. A preliminary validation of these data was also conducted. Immatures of M. scalaris were reared on either bovine or porcine biceps femoris at 24 C, 28 C, and 32 C. Temperature significantly im- pacted immature development time, including egg eclosion, eclosion to pupation, and pupation to adult emer- gence, to favor faster development at higher temperatures. From ovipostion to eclosion, development rate was 32.1% faster from 24 Cto28C, 13.9% faster from 28 Cto32C, and 45.5% faster from 24 Cto32C. Development from eclosion to pupation displayed similar results with differences of 30.3% between 24 C and 28 C, 15.4% between 28 C and 32 C, and 45.2% between 24 C and 32 C. Development from pupation to adult emergence, likewise, displayed a 44.4% difference from 24 C and 28 C, 7.3% from 28 Cto32C, and 51.2% from 24 Cto32 C. From oviposition to adult emergence, M. scalaris needed 32.7% more hours to complete by guest on March 30, 2016 development when reared at 24 C than 28 C, 8.5% when reared at 28 C rather than 32 C, and 38.4% more time when reared at 24 C over 32 C. Tissue type did not significantly impact development. A preliminary validation study was conducted in four indoor environments (two attics, a closet, and a bathroom) spanning two different buildings. Utilizing minimum and mean lengths, time of colonization estimates were underestimated in all instances. The predicted range encompassed the actual TOC for two of the four environ- ments. On average, when using minimum length, time of colonization was underestimated by 45%, but overes- timated by only 2% when using maximum development range. Data generated from this research could be useful when estimating a TOC of decomposing vertebrate remains. Future research will need to examine devel- opment for each stadium in order to increase precision of such estimates. Key words: forensic entomology, development data, postmortem interval, coffin fly Forensic entomology is the utilization of insects and other arthro- (Dipera: Sarcophagidae) (Singh and Sharma 2008), and phorids pods as evidence in both civil and criminal investigations (Smith (Disney 2008) collected from decedents as well as the living 1986), as well as within noncriminal investigations of death or myia- (Anderson and Huitson 2004). Although similar development times sis. The broad scope of this field can be broken into three subdiv- have been documented in pre-existing data sets for a variety of blow isions of urban, stored-product, and medicolegal forensic fly species, some variations can be found within species (Tarone and entomology (Archer 2007). Medicolegal forensic entomology relates Foran 2006, Gallagher et al. 2010, Owings et al. 2014). Variation to necrophagous arthropods colonizing and feeding on living and could be due to a number of factors, such as experiment design, en- necrotic human and animal tissue. Insects found on vertebrate re- vironment, and genetic variation (Tarone and Foran 2006). mains may be utilized to estimate the time of colonization (TOC; Most development data utilized by forensic entomologists are Amendt et al. 2007). obtained from studies examining the growth of larvae fed liver of Forensic entomologists depend on development data from la- various mammalian species (Clark et al. 2006). However, it has boratory studies to estimate the TOC for insects, such as blow flies been demonstrated that the host species on which the larvae feed (Diptera: Calliphoridae) (Tarone and Foran 2006), flesh flies may significantly alter larval growth rate. For example, development VC The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: [email protected] 1 2 Journal of Medical Entomology, 2016, Vol. 0, No. 0 of Lucilia sericata (Meigen) (Diptera: Calliphoridae) fed lung, liver, the Harris County Institute of Forensic Sciences in October 2014. and heart, from both cows and swine were compared (Clark et al. Larvae were provided 50 g of fresh bovine liver within their trans- 2006). Larvae grew significantly faster on porcine than on bovine portation container and allowed to pupate. tissue. Furthermore, development varied when reared on lung and Resulting adult flies were housed in a 30- by 30- by 30-cm nylon heart compared with liver of the same animal. For forensic ento- mesh arthropod-rearing cage (BioQuip Productes Inc., Rancho mologists, such results emphasize the need for development data sets Dominguez, CA) and provided a 50:50 sugar:powdered milk mix- across tissue types as well as across species tissue source. The im- ture and fresh water ad libitum. The room was maintained at 24– portance of recording the location from which insect larvae are 28 C and a photoperiod of 12:12 (L:D) h in the Forensic removed from a corpse was also demonstrated (Clark et al. 2006). Laboratory of Investigative Entomological Sciences (F.L.I.E.S.) Such information can allow for refined estimates of the TOC. Facility at Texas A&M University, College Station, Texas, USA. Megaselia scalaris (Loew) (Diptera: Phoridae) is widely encoun- Approximately 50 g of bovine liver in a 140 mm wide by 20 mm tered in forensic entomology investigations; however, little is known deep petri dish was provided to the colony for three hours as an ovi- about the biology of this species. Phoridae are commonly known as position substrate. Resulting eggs and associated liver were placed hunchback flies, scuttle flies, or coffin flies, due in part to their in a 236.5-ml, wide mouth Kerr Mason jar (Kerr Group Inc., morphology, behavior, and food source, respectively. Cosmopolitan Lancaster, PA) and reared under the same conditions previously by nature, M. scalaris can be a scavenger (Disney 2008), parasite mentioned. Emerged adults were collected daily and placed in a cage (Miranda-Miranda et al. 2011), or detritivore (Koch et al. 2012). similar to the one previously described. Utilizing diverse habitats from tropical rain forests to heavily urban- ized areas, the wide distribution of M. scalaris makes it both a resi- Source Downloaded from dential pest, and an excellent candidate for forensic entomology The biceps femoris muscle from remains of two bovines, Bos taurus casework (Disney 2012). This species has been encountered on (L.), and two porcines, Sus scrofa (L.), was utilized in this develop- human remains located indoors in a sealed building, where the small ment study. All tissue was acquired from a local butcher shop size of the phorids allowed them access to a corpse where access by (Readfield Meats and Deli, College Station, TX), and were free of blow flies was restricted (Reibe and Madea 2010). barbiturates or other materials used for euthanization. Each muscle http://jme.oxfordjournals.org/ Though development data exist for M. scalaris, these experiments was cut into 50-g pieces varying in shape and stored frozen at are based on populations either from other countries besides the USA, À20 C in appropriately labelled Ziploc bags (S.C. Johnson and Son such as Mexico (Miranda-Miranda et al. 2011) and Malaysia (Zuha Inc., Racine, WI). All protocols were approved by the Texas A&M and Omar 2014), or from regions in North America other than Texas, University Institutional Animal Care and Use Committee (IACUC) USA (Illinois: Greenberg and Wells 1998,Illinois:Prawirodisastro and and Animal Welfare Assurance Program. Benjamin 1979, Virginia: Trumble and Pienkowski 1979). Likewise, there are concerns that genetic differences between populations may result in development differences and caution should be exercised Experiment Design Methods were based on those of Boatright and Tomberlin (2010). when applying these data to cases outside these regions and climates. by guest on March 30, 2016 Such differences have already been recorded with oriental latrine flies Tissue samples were removed from the freezer and allowed to thaw Chrysomya megacephala (F.) (Diptera: Calliphoridae), where popula- and reach room temperature prior to use. Approximately 50 g of ei- tions from different regions in China along the same latitudinal gradi- ther bovine or porcine tissue was placed in a 236.5-ml wide mouth ent within different environments demonstrated slowed or increased Kerr Mason jar filled a quarter way with sand (Quikrete Premium overall development times (Hu et al. 2010). The same has been deter- Play Sand, Quikrete Companies Inc., Atlanta, GA) to prevent pool- mined for Cochliomyia macellaria (F.) (Diptera: Calliphoridae) from ing of decomposition fluids. Previous observations showed that 50 g different regions in Texas, USA (Owings et al. 2014)andL. sericata of the respective tissues were more than adequate for rearing pur- from different regions of the USA (Gallagher et al.
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