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A Multi-Mode Nano Isolation System for Preferential Hemoglobin

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A Multi-Mode Nano Isolation System for Preferential Hemoglobin Electronic Supplementary Material (ESI) for Journal of Materials Chemistry B. This journal is © The Royal Society of Chemistry 2016 Supporting Information An Octamolybdate-Metal Organic Framework Hybrid for the Efficient Adsorption of Histidine-rich Proteins Qing Chen, Meng-Meng Wang, Xue Hu, Xu-Wei Chen*, Jian-Hua Wang* Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China *E-mail address: [email protected] (X.W. Chen), [email protected] (J.H. Wang). 1 Table S1. The number of total proteins identified in human plasma sample after depleting high abundant proteins by the Mo8O26@MIL-101 hybrid. No. Peptides≥2. Protein Name MW [kDa] calc. pI Afamin OS=Homo sapiens 1 P43652 69.024 5.9 GN=AFM PE=1 SV=1 Alpha-1-acid glycoprotein 1 2 P02763 OS=Homo sapiens GN=ORM1 23.497 5.02 PE=1 SV=1 Alpha-1-acid glycoprotein 2 3 P19652 OS=Homo sapiens GN=ORM2 23.588 5.11 PE=1 SV=2 Alpha-1-antitrypsin OS=Homo 4 A0A024R6I7 sapiens GN=SERPINA1 PE=1 46.679 5.59 SV=1 Alpha-1-antitrypsin OS=Homo 5 P01009 sapiens GN=SERPINA1 PE=1 46.707 5.59 SV=3 Alpha-2-antiplasmin 6 P08697 OS=Homo sapiens 54.531 6.29 GN=SERPINF2 PE=1 SV=3 Alpha-2-macroglobulin 7 P01023 OS=Homo sapiens GN=A2M 163.188 6.46 PE=1 SV=3 Amyloid lambda 6 light chain variable region NEG 8 Q96JD2 11.901 4.94 (Fragment) OS=Homo sapiens PE=2 SV=1 Amyloid lambda 6 light chain variable region SAR 9 Q96JD0 12.287 5.01 (Fragment) OS=Homo sapiens PE=2 SV=1 Angiotensinogen variant 10 Q59EP2 (Fragment) OS=Homo sapiens 53.744 6.42 PE=2 SV=1 Anti-folate binding protein 11 A2NYQ9 (Fragment) OS=Homo sapiens 13.274 8.44 GN=HuVH8B VH PE=2 SV=1 Anti-H1N1 influenza HA 12 G3GAU4 11.599 7.96 kappa chain variable region 2 (Fragment) OS=Homo sapiens PE=2 SV=1 Anti-Influenza A hemagglutinin heavy chain 13 G1FM85 14.402 8.41 variable region (Fragment) OS=Homo sapiens PE=2 SV=1 Anti-mucin1 heavy chain 14 A2JA14 variable region (Fragment) 13.9 5.41 OS=Homo sapiens PE=2 SV=1 Anti-mucin1 light chain 15 A2JA19 variable region (Fragment) 11.651 8.91 OS=Homo sapiens PE=2 SV=1 Anti-mucin1 light chain 16 A2JA16 variable region (Fragment) 11.614 8.46 OS=Homo sapiens PE=2 SV=1 Anti-streptococcal/anti-myosin immunoglobulin kappa light 17 Q96SA9 chain variable region 11.513 8.85 (Fragment) OS=Homo sapiens PE=2 SV=1 Anti-streptococcal/anti-myosin immunoglobulin lambda light 18 Q96SB0 chain variable region 11.587 6.54 (Fragment) OS=Homo sapiens PE=2 SV=1 Anti-thyroglobulin heavy chain 19 Q8IZD7 variable region (Fragment) 13.893 8.46 OS=Homo sapiens PE=2 SV=1 Anti-TN-C scFv (Fragment) 20 A2KBC4 25.148 8.41 OS=Homo sapiens PE=2 SV=1 APOL1 protein (Fragment) 21 A5PL32 OS=Homo sapiens 48.486 7.17 GN=APOL1 PE=2 SV=1 Apolipoprotein A-I OS=Homo 22 P02647 sapiens GN=APOA1 PE=1 30.759 5.76 SV=1 Apolipoprotein A-II OS=Homo 23 V9GYM3 sapiens GN=APOA2 PE=1 14.905 8.27 SV=1 Apolipoprotein A-IV 24 P06727 OS=Homo sapiens 45.371 5.38 GN=APOA4 PE=1 SV=3 Apolipoprotein C-I, isoform 25 A0A024R0T8 9.326 8.47 CRA_a OS=Homo sapiens 3 GN=APOC1 PE=4 SV=1 Apolipoprotein D (Fragment) 26 C9JF17 OS=Homo sapiens GN=APOD 24.142 5.6 PE=1 SV=1 Apolipoprotein E OS=Homo 27 P02649 sapiens GN=APOE PE=1 36.132 5.73 SV=1 Apolipoprotein M OS=Homo 28 O95445 sapiens GN=APOM PE=1 21.239 6.01 SV=2 Attractin OS=Homo sapiens 29 O75882 158.432 7.31 GN=ATRN PE=1 SV=2 Beta-2-glycoprotein 1 30 P02749 OS=Homo sapiens GN=APOH 38.273 7.97 PE=1 SV=3 Biotinidase OS=Homo sapiens 31 P43251 61.093 6.25 GN=BTD PE=1 SV=2 Cadherin 5, type 2 preproprotein variant 32 Q59EA3 89.868 5.48 (Fragment) OS=Homo sapiens PE=2 SV=1 Carboxylic ester hydrolase 33 D3DNN4 OS=Homo sapiens GN=BCHE 72.836 7.55 PE=3 SV=1 CD5 antigen-like OS=Homo 34 O43866 sapiens GN=CD5L PE=1 38.063 5.47 SV=1 cDNA FLJ14473 fis, clone MAMMA1001080, highly similar to Homo sapiens 35 Q96K68 53.054 6.86 SNC73 protein (SNC73) mRNA OS=Homo sapiens PE=2 SV=1 CDNA FLJ26301 fis, clone 36 Q6ZP85 DMC07540 OS=Homo sapiens 25.787 8.38 PE=2 SV=1 CDNA FLJ26936 fis, clone 37 Q6ZNX5 RCT06808 OS=Homo sapiens 17.663 7.52 PE=2 SV=1 cDNA FLJ35730 fis, clone TESTI2003131, highly similar 38 B3KS79 to ALPHA-1- 50.596 5.63 ANTICHYMOTRYPSIN OS=Homo sapiens PE=2 SV=1 4 cDNA FLJ41981 fis, clone SMINT2011888, highly 39 Q6ZVX0 similar to Protein Tro alpha1 52.841 5.92 H,myeloma OS=Homo sapiens PE=2 SV=1 cDNA FLJ53691, highly 40 B4E1B2 similar to Serotransferrin 74.783 7.12 OS=Homo sapiens PE=2 SV=1 cDNA FLJ55606, highly similar to Alpha-2-HS- 41 B7Z8Q2 46.597 6.28 glycoprotein OS=Homo sapiens PE=2 SV=1 cDNA FLJ58124, highly similar to Complement factor I 42 B4DRF2 66.651 7.52 (EC 3.4.21.45) OS=Homo sapiens PE=2 SV=1 cDNA FLJ75881, highly similar to Homo sapiens 43 A8K6Q8 transferrin receptor (p90, 84.82 6.61 CD71) (TFRC), mRNA OS=Homo sapiens PE=2 SV=1 cDNA FLJ76826, highly similar to Homo sapiens 44 A8K5A4 ceruloplasmin (ferroxidase) 122.095 5.74 (CP), mRNA OS=Homo sapiens PE=2 SV=1 cDNA FLJ77835, highly similar to Homo sapiens complement component 1, s 45 A8K2N0 76.563 4.98 subcomponent (C1S), transcript variant 2, mRNA OS=Homo sapiens PE=2 SV=1 cDNA FLJ78071, highly similar to Human MHC class 46 A8K8Z4 III complement component C6 104.649 6.62 mRNA OS=Homo sapiens PE=2 SV=1 cDNA FLJ78207, highly similar to Human complement 47 A8K2T4 93.346 6.51 protein component C7 mRNA OS=Homo sapiens PE=2 SV=1 cDNA FLJ78387 OS=Homo 48 A8K008 51.564 8.16 sapiens PE=1 SV=1 5 cDNA FLJ90170 fis, clone MAMMA1000370, highly 49 Q8NCL6 similar to Ig alpha-1 chain C 53.19 6.52 region OS=Homo sapiens PE=2 SV=1 cDNA, FLJ93141, highly similar to Homo sapiens 50 B2R6V9 coagulation factor XIII, A1 83.189 6 polypeptide (F13A1), mRNA OS=Homo sapiens PE=2 SV=1 cDNA, FLJ93387, highly similar to Homo sapiens 51 B2R7D1 transglutaminase 4 (prostate) 77.08 6.76 (TGM4), mRNA OS=Homo sapiens PE=2 SV=1 cDNA, FLJ93914, highly similar to Homo sapiens 52 B2R8I2 histidine-rich glycoprotein 59.475 7.44 (HRG), mRNA OS=Homo sapiens PE=2 SV=1 cDNA, FLJ94361, highly similar to Homo sapiens serine (or cysteine) proteinase inhibitor, clade A(alpha-1 53 B2R9F2 45.066 6.04 antiproteinase, antitrypsin), member 6 (SERPINA6), mRNA OS=Homo sapiens PE=2 SV=1 cDNA, FLJ94686, highly similar to Homo sapiens 54 B2RA39 complement factor H-related 5 64.349 7.06 (CFHL5), mRNA OS=Homo sapiens PE=2 SV=1 Clusterin OS=Homo sapiens 55 P10909 52.461 6.27 GN=CLU PE=1 SV=1 Coagulation factor X 56 P00742 OS=Homo sapiens GN=F10 54.697 5.94 PE=1 SV=2 Coiled-coil domain-containing 57 Q8NCX0 protein 150 OS=Homo sapiens 128.681 7.02 GN=CCDC150 PE=1 SV=2 Cold agglutinin FS-1 L-chain 58 A2NB45 (Fragment) OS=Homo sapiens 12.38 8.48 PE=2 SV=1 6 Cold agglutinin FS-2 H-chain 59 A2NB44 (Fragment) OS=Homo sapiens 12.753 7.11 GN=IGH@ PE=2 SV=1 Complement C4-A OS=Homo 60 A0A0G2JPR0 192.754 7.03 sapiens GN=C4A PE=1 SV=1 Complement component 9, 61 A0A024R035 isoform CRA_a OS=Homo 63.163 5.52 sapiens GN=C9 PE=4 SV=1 Complement component C8 62 P07357 alpha chain OS=Homo sapiens 65.121 6.47 GN=C8A PE=1 SV=2 Cryocrystalglobulin CC1 heavy chain variable region 63 B1N7B6 13.184 8.48 (Fragment) OS=Homo sapiens PE=2 SV=1 Cryocrystalglobulin CC2 lambda light chain variable 64 B1N7B9 11.308 7.97 region (Fragment) OS=Homo sapiens PE=2 SV=1 C-type lectin domain family 3, A0A024R2Q member B, isoform CRA_a 65 22.522 5.67 7 OS=Homo sapiens GN=CLEC3B PE=4 SV=1 Cysteine-rich secretory protein 66 J3KPA1 3 OS=Homo sapiens 30.955 7.61 GN=CRISP3 PE=1 SV=1 Dermcidin OS=Homo sapiens 67 P81605 11.277 6.54 GN=DCD PE=1 SV=2 Dopamine beta-hydroxylase 68 P09172 OS=Homo sapiens GN=DBH 69.021 6.42 PE=1 SV=3 Dynein heavy chain 14, 69 Q0VDD8 axonemal OS=Homo sapiens 399.64 6.93 GN=DNAH14 PE=2 SV=3 Epididymis luminal protein 180 (Fragment) OS=Homo 70 B6EDE2 13.28 5.45 sapiens GN=HEL180 PE=2 SV=1 Epididymis luminal protein 71 V9HW68 214 OS=Homo sapiens 51.683 7.75 GN=HEL-214 PE=2 SV=1 Epididymis secretory sperm 72 V9HWD8 binding protein Li 163pA 54.22 5.86 OS=Homo sapiens GN=HEL- 7 S-163pA PE=2 SV=1 Epididymis secretory sperm binding protein Li 62p 73 V9HWA9 187.03 6.4 OS=Homo sapiens GN=HEL- S-62p PE=2 SV=1 F5-20 (Fragment) OS=Homo 74 A0N7I9 13.411 9.1 sapiens GN=F5-20 PE=2 SV=1 A0A0M3KK Fab Hu 15C1 Heavy chain 75 OS=Homo sapiens PE=1 SV=1 24.059 8.85 W6 Fibulin-1 OS=Homo sapiens 76 P23142 77.162 5.22 GN=FBLN1 PE=1 SV=4 FLJ00382 protein (Fragment) 77 Q8NF20 OS=Homo sapiens 52.892 8.66 GN=FLJ00382 PE=2 SV=1 FLJ00385 protein (Fragment) 78 Q8NF17 OS=Homo sapiens 56.075 7.59 GN=FLJ00385 PE=1 SV=1 Full-length cDNA clone CS0DD006YL02 of 79 Q86TT1 Neuroblastoma of Homo 41.247 6.79 sapiens (human) OS=Homo sapiens PE=2 SV=1 Gelsolin OS=Homo sapiens 80 P06396 85.644 6.28 GN=GSN PE=1 SV=1 Glutathione peroxidase 3 81 P22352 OS=Homo sapiens GN=GPX3 25.537 8.13 PE=1 SV=2 Haptoglobin OS=Homo 82 P00738 45.177 6.58 sapiens GN=HP PE=1 SV=1 Haptoglobin-related protein 83 P00739 OS=Homo sapiens GN=HPR 39.005 7.09 PE=2 SV=2 Heavy chain Fab (Fragment) 84 A2NYU7 13.983 9.25 OS=Homo sapiens PE=2 SV=1 Heavy chain Fab (Fragment) 85 A2NYU8 12.667 9.2 OS=Homo sapiens PE=2 SV=1 Heavy chain Fab (Fragment) 86 A2NYV1 14.271 6.54 OS=Homo sapiens PE=2 SV=1 Heavy chain Fab (Fragment) 87 A2NYV0 13.25 9.29 OS=Homo sapiens PE=2 SV=1 Heavy chain of factor I 88 Q6LAM1 (Fragment) OS=Homo sapiens 35.859 7.59 PE=2 SV=1 8 Heavy chain variable region 89 A2NXP8 (Fragment) OS=Homo sapiens 13.825 9.72 PE=2 SV=1 Hemoglobin, beta OS=Homo 90 D9YZU5 15.988 7.28 sapiens GN=HBB PE=3 SV=1 Hemopexin OS=Homo sapiens 91 P02790 51.643 7.02 GN=HPX PE=1 SV=2 Heparin cofactor 2 OS=Homo 92 P05546 sapiens GN=SERPIND1 PE=1 57.034
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