International Journal of Molecular Sciences Article Dual RNA Sequencing of Vitis vinifera during Lasiodiplodia theobromae Infection Unveils Host–Pathogen Interactions Micael F. M. Gonçalves 1 , Rui B. Nunes 1, Laurentijn Tilleman 2 , Yves Van de Peer 3,4,5 , Dieter Deforce 2, Filip Van Nieuwerburgh 2, Ana C. Esteves 6 and Artur Alves 1,* 1 Department of Biology, CESAM, University of Aveiro, 3810-193 Aveiro, Portugal;
[email protected] (M.F.M.G.);
[email protected] (R.B.N.) 2 Laboratory of Pharmaceutical Biotechnology, Campus Heymans, Ottergemsesteenweg 460, B-9000 Ghent, Belgium;
[email protected] (L.T.);
[email protected] (D.D.);
[email protected] (F.V.N.) 3 Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium;
[email protected] 4 Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium 5 Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Pretoria 0028, South Africa 6 Faculty of Dental Medicine, Center for Interdisciplinary Research in Health (CIIS), Universidade Católica Portuguesa, Estrada da Circunvalação, 3504-505 Viseu, Portugal;
[email protected] * Correspondence:
[email protected]; Tel.: +351-234-370-766 Received: 28 October 2019; Accepted: 29 November 2019; Published: 3 December 2019 Abstract: Lasiodiplodia theobromae is one of the most aggressive agents of the grapevine trunk disease Botryosphaeria dieback. Through a dual RNA-sequencing approach, this study aimed to give a broader perspective on the infection strategy deployed by L. theobromae, while understanding grapevine response. Approximately 0.05% and 90% of the reads were mapped to the genomes of L.