Volume 10, Issue 1, 2020, 4811 - 4820 ISSN 2069-5837 Biointerface Research in Applied Chemistry www.BiointerfaceResearch.com https://doi.org/10.33263/BRIAC101.811820 Original Research Article Open Access Journal Received: 30.09.2019 / Revised: 16.12.2019 / Accepted: 20.12.2019 / Published on-line: 28.12.2019 Thermoflavimicrobium dichotomicum as a novel thermoalkaliphile for production of environmental and industrial enzymes Osama M. Darwesh 1,2,* , Islam A. Elsehemy 3, Mohamed H. El-Sayed 4,5 , Abbas A. El-Ghamry 4 , Ahmad S. El-Hawary 4 1Agricultural Microbiology Dept., National Research Centre, Dokki, Cairo, Egypt 2School of Material Sciences and Engineering, Hebei University of Technology, 300130 Tianjin, China 3Department of Natural and Microbial Products Chemistry, National Research Centre, Dokki, Cairo, Egypt 4Botany and Microbiology Dept., Faculty of Science (Boys); Al-Azhar University, Nasr city, Cairo, Egypt 5Department of Biology, Faculty of Science and Arts, Northern Border University (Rafha), Kingdom of Saudi Arabia *corresponding author e-mail address:
[email protected] | Scopus ID 56128096000 ABSTRACT Thermoalkaliphilic actinomycetes enzymes have many important applications in many industrial, biotechnological and environmental aspects. So, the current study aimed to obtain the thermoalkali-enzymes producing actinomycetes. A novel thermoalkaliphilic actinomycete strain was isolated from Egyptian Siwa oasis and identified according to its morphological, physiological and biochemical characters as Thermoflavimicrobium dichotomicum. And then confirmed by phylogenetic analysis and the partial sequence was deposited in GenBank under accession number of KR011193 and name of Thermoflavimicrobium dichotomicum HwSw11. It could produce amylase, cellulase, lipase, pectinase and proteinase enzymes. Also, this strain exhibited anti-bacterial activities against P. aeruginosa and E.