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5Th Annual RI SURF Conference Abstract Book, Friday, July 27, 2012

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5Th Annual RI SURF Conference Abstract Book, Friday, July 27, 2012 2012 RHODE ISLAND SUMMER UNDERGRADUATE RESEARCH FELLOWSHIP CONFERENCE Friday, July 27, 2012 8:00 AM CENTER FOR BIOTECHNOLOGY & LIFE SCIENCES UNIVERSITY OF RHODE ISLAND Supported by RI-INBRE & RI EPSCOR SUMMER UNDERGRADUATE RESEARCH FELLOWS (SURF) CONFERENCE FRIDAY, JULY 27, 2012 CENTER FOR BIOTECHNOLOGY & LIFE SCIENCES UNIVERSITY OF RHODE ISLAND KINGSTON, RI 8:00 – 8:45 AM CONTINENTAL BREAKFAST & SURF GROUP A POSTER SET-UP 8:45 – 9:10 AM WELCOMING REMARKS 9:10 – 10:10 AM SURF POSTER SESSION - GROUP A 10:10 -10:20 AM INTERMISSION I & SURF GROUP B POSTER SET-UP 10:20 – 11:20 AM SURF POSTER SESSION - GROUP B 11:20 -11:30 AM INTERMISSION II & SURF GROUP C POSTER SET-UP 11:30 – 12:30 PM SURF POSTER SESSION - GROUP C 12:30 PM LUNCH LIST OF SUMMER RESEARCH FELLOW POSTERS **Please note that the poster numbers listed in the following tables also correspond with the page numbers in the abstract book. Students are listed under the institution where their research was conducted. University of Rhode Island Poster # Summer Fellow Mentor 1 Soliel Doman Aftab Ahmed 100 James Stevenson Clinton Chichester 12 Lauren Boltz Geoffrey Bothun 123 Farid Topchiev 92 Annalisa Sharkey Bongsup Cho 74 Stephen Norris 132 Jillian Zoglio Brenton DeBoef 26 Tori Deschenes Anne DeGroot 18 Jeff Chau 30 Evan Dunphy Caroline Gottschalk Druschke 30 Peter Schooling 45 Allison Holevoet 68 Sarah Merolla Graham Forrester 16 Jeremy Carreiro Art Gold 3 Samantha Nicodemus Niall Howlett 88 Ryann Rossi Brita Jessen 25 Alyssa Dantonio Roberta King 110 Kevin Sun Abraham Kovoor 109 Elizabeth Sullivan 24 Melisa Curran Christopher Lane 85 Alyssa Rogers 108 Madeleine Suits Mindy Levine 116 Kevin Szulak Wei Lu University of Rhode Island (continued) Poster # Summer Fellow Mentor 91 Eric Shabashevich David Nelson 75 Kevin Northup Keykavous Parang 125 Holly Tran Yana Reshetnyak 60 Zachary Lariviere David Rowley 56 Kristen Knoph Navindra Seeram 94 Adam Silva Angela Slitt 10 Emily Bishop Carol Thornber 51 Hannah Jones Hiro Uchida 117 Rachel Thakore Daniel Udwary Brown University Poster # Summer Fellow Mentor 90 Christian Selinski Edward Hawrot 107 Elena Suglia Gary Wessel 119,120,188 Cynthia Gaudet Barbara Stonestreet 67 Nicholas DeLeo John Marshall 11 Eimear Black Amit Basu Bryant University Poster # Summer Fellow Mentor 73 Christina Nadolny Kirsten Hokeness & 70 Ryan Miller Christopher Reid 58 Jacqueline Kratch 129 Zoe White Dan McNally 54 Trevor Kent Julia Crowley Parmentier 46 Garrett Holmes Christopher Reid Providence College Poster # Summer Fellow Mentor 48,86 Matthew Hurton 86 Stephen Rogers Nicanor Austriaco 34 Ryan Frazier 133 Natasha Zupkus Maia Bailey 29,83 Brenna Peters 29,83 Caroline Doyle Christopher Bloom 29,83 Ryan Post 21 Eliza Conaty 78 Ryan Paranal Joseph DeGiorgis 67 Nicholas Marcello 126 Lauren Trotta Patrick Ewanchuk 121 Matthew DeBlois Jeffrey Markert 122 Kelsey Garlick 80 Meaghan Keane 80 Zach Sexton Brett Pellock 80 Matt Goulet 80 Taylor Hunt 61 Alexandra Male 61 Christina Taylor Jennifer Van Reet 61 Christina Lavigne Rhode Island College Poster # Summer Fellow Mentor 19 Kristen Chauvin 35,82 Christopher Funk Karen Almeida 35,82 Cailyn Mather 35,82 Katelyn Pina 31,47,50 Masharee Hopkins-Jones 31,47,50 Sabrina Elgar Deborah Britt 31,47,50 Kyle Inman 22 Kristen Wilkinson 22 Kyle Fernandes Emily Cook 22 Kayla Flynn 39 Kirstie Lepore 39 Kevin Fornari Beverly Goldfield 39 Christina Gencarella 89 Kyle Schoolcraft Brea Govenar 55,66 Lorin Kinney 66,124 Rachel Traghella Thomas Malloy 66 Brandon DeSimone 81 Kaitlyn O’Connor Thomas Meedel 81 Clifford Picket Rhode Island College (continued) Poster # Summer Fellow Mentor 101 Irina Maglysh 101 Sabiha Rahman Sarah Spinette 101 Jacklyn Lata 118,120 Nicholas Lafond 118,120 Katrina Feyerherm Steven Threlkeld 118,120 Molly LaRue 5 Duane Barnes 15 Titalayo Adedji-Campbell John Williams 64 Jose Lora Rhode Island School of Design Poster # Summer Fellow Mentor 102 Mengzhou Li Neal Overstrom 102 Eliza Squibb Roger Williams University Poster # Summer Fellow Mentor 65 Kelsey Lucas Sean Colin 65 Eric Klos 33 Hanna Sobon 33 Kevin Schindelwig Franca Avelina Espinosa 33 Layla Ferland 71 Andrew Mitchell 131 Alicia Wilson Lonnie Guralnick 79 Christopher Pellichero Dale Leavitt 17 Peter Cavedon Kathryn Markey 40,52 Joshua Jones Andrew Rhyne 40,52 Allex Gourlay 41 Catherine Grimm Roxonna Smolowitz 43 Allison Hall 59 Nicholas Kutil David Taylor 62 Garrett LeBlanc 77 Michael Pallotta 105, 106 Laura Stevenson Kerri Warren 105, 106 Janani Subramaniam Salve Regina University Poster # Summer Fellow Mentor 49 Alexandra Igo 8 Nicole Bickford Jameson Chace 57 Olivia Kopin 127 Madison Van Orden James Diamontopoulos Sarah Materese 32 Caitlyn Farragher 93 Sarah Showalter 38 Mallory Goding Susan Meschwitz 13 Taylor Braun 99 Brian Somba 96 Morgan Smith Bernard Munge 4 Alexander Antonopoulos 95 Bridget Smith 23 Tia Crowther 112 Stephanie Beels Alison Shakarian 2,95 Erika Albretsen 23, 44 Lana Hoertz 114,115 Craig Irving 115 Karl Varkey Steven Symington 114 Justin Gay 114 Wayne Bainter Salve Regina University (continued) 9,42, 103 Alexandria Bierce 42, 103 Alyssa Guarracino 9,42, 103 Kelsey Stafstrom 6,14,36 Amy Battocletti John-David Swanson 6,14,36 Shennel Gelin 14 Matthew Breseman PURIFICATION AND PARTIAL CHARACTERIZATION OF SNAKE LEAF-NOSED VIPER (ERISTICOPHIS MACMAHONII ) HEMOGLOBIN Soliel Doman, Department of Biotechnology, Community College of Rhode Island, Warwick, RI; Humera Faraz, Iqbal Chaudhary, HEJ Research Institute of Chemistry, University of Karachi, Karachi, Pakistan; Aftab Ahmed, Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston, RI RI-INBRE Summer Undergraduate Research Fellowship Program Hemoglobin is a respiratory protein present in the erythrocytes of all vertebrae. Typically, it consists of two identical pairs of α and β globin chains. Our preliminary investigation on snake Leaf-Nosed Viper hemoglobin is being conducted with the intent to characterize and better understand the interspecies relationships among various snakes at the molecular level. The Leaf-Nosed Viper is a deadly poisonous snake, classified in the family Viperidae, represented in Pakistan by five genera, seven species, and subspecies. The hemoglobin was isolated from the washed RBCs with physiological saline and the globin was further isolated by treatment in cold acidified acetone. The globin chains were separated by reversed-phase HPLC. The corresponding βII globin chain was oxidized and digested with TPCK treated trypsin. Peptides were separated by RP-HPLC, and homogeneity of the peptides was checked by MALDI-TOF mass spectrometry. Partial amino acid sequence of βII chain was deduced by Edman degradation of the intact globin chain and of the purified tryptic peptides in an automated protein sequencer. The primary structure of the βII chain of the LNV was aligned to other reported βII chain sequences, and it was found to be highly conserved. 1 DETERMINING DIFFERENCES IN PROTEIN EXPRESSION PATTERNS AMONG FOUR DIFFERENT LEISHMANIA SPECIES THROUGH CDNA CLONING AND SEQUENCING Erika Albretsen, Department of Biology, Salve Regina University, Newport, RI; Alison Shakarian, Department of Biology and Biomedical Sciences, Salve Regina University, Newport, RI RI EPSCoR Summer Undergraduate Research Fellowship Program Leishmania is a protozoan parasite of the Trypanasomatidae family that can cause two different disease manifestations in humans. Cutaneous leishmaniasis causes ulcerating skin lesions and is generally found to be associated with L. mexicana and L. major. Visceral leishmaniasis causes inflammation of the liver, spleen, and bone marrow and is found to be associated with L. donovani. On the other hand, it has been found that L. tarentolae is not pathogenic and does not cause either of the two manifestations. The purpose of this research is to determine if there are different proteins expressed by these organisms that may be a contributing factor in the varying pathogenicities associated with the four Leishmania species. The total RNA of each species was isolated and cDNA was synthesized. Amplified Fragment Length Polymorphisms (AFLP) reactions with 6 selective primer sets were completed to identify polymorphic and unique cDNA fragments among the four Leishmania species.. These unique cDNA-AFLP fragments were cloned into pCR- TOPO plasmid vectors using E. coli. Colonies were isolated and subjected to PCR to determine if the size of cDNA fragments matched the originally cloned AFLP fragments. The plasmids were subsequently isolated, sequenced and subjected to a nucleotide BLAST search. Results showed genes for ribosomal and unknown hypothetical proteins expressed by these parasites in a species specific manner. Interestingly, it was also found that the parasites differentially expressed genes for superoxide dismutase, calreticulin, aminopeptidase and an ATP-binding cassette protein. Additional cDNA-AFLP fragments are currently being isolated and sequenced. Future experiments include verification of differential expression of the genes identified by AFLP analysis using real time qPCR. 2 CHARACTERIZATION OF A PUTATIVE HISTONE H4 BINDING DOMAIN IN THE FANCONI ANEMIA D2 PROTEIN Samantha Nicodemus, Department of Cell and Molecular Biology, Community College of Rhode Island, Warwick, RI; Karissa Neira, Nial Howlett, Department of Cell and Molecular Biology, University of Rhode Island, Kingston, RI RI-INBRE Summer Undergraduate Research Fellowship Program Fanconi Anemia (FA) is a rare genetic disorder characterized by bone marrow
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