SNX15 Rabbit Pab

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Leader in Biomolecular Solutions for Life Science SNX15 Rabbit pAb Catalog No.: A9158 Basic Information Background Catalog No. This gene encodes a member of the sorting nexin family. Members of this family contain A9158 a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. Overexpression of this gene results in a decrease in the Observed MW processing of insulin and hepatocyte growth factor receptors to their mature subunits. 50kDa This decrease is caused by the mislocalization of furin, the endoprotease responsible for cleavage of insulin and hepatocyte growth factor receptors. This protein is involved in Calculated MW endosomal trafficking from the plasma membrane to recycling endosomes or the trans- 29kDa/38kDa Golgi network. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ADP-ribosylation factor- Category like 2 (ARL2) gene. Primary antibody Applications WB,IF Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 29907 Q9NRS6 IF 1:50 - 1:200 Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human SNX15 (NP_037438.2). Synonyms SNX15;HSAF001435 Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using SNX15 antibody (A9158) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. Immunofluorescence analysis of C6 cells Immunofluorescence analysis of HeLa cells Immunofluorescence analysis of L929 cells using SNX15 antibody (A9158) at dilution using SNX15 antibody (A9158) at dilution using SNX15 antibody (A9158) at dilution of 1:100. Blue: DAPI for nuclear staining. of 1:100. Blue: DAPI for nuclear staining. of 1:100. Blue: DAPI for nuclear staining. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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Sorting Nexins in Protein Homeostasis Sara E. Hanley1,And Katrina F
Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 6 November 2020 doi:10.20944/preprints202011.0241.v1 Sorting nexins in protein homeostasis Sara E. Hanley1,and Katrina F. Cooper2* 1Department of Molecular Biology, Graduate School of Biomedical Sciences, Rowan University, Stratford, NJ, 08084, USA 1 [email protected] 2 [email protected] * [email protected] Tel: +1 (856)-566-2887 1Department of Molecular Biology, Graduate School of Biomedical Sciences, Rowan University, Stratford, NJ, 08084, USA Abstract: Sorting nexins (SNXs) are a highly conserved membrane-associated protein family that plays a role in regulating protein homeostasis. This family of proteins is unified by their characteristic phox (PX) phosphoinositides binding domain. Along with binding to membranes, this family of SNXs also comprises a diverse array of protein-protein interaction motifs that are required for cellular sorting and protein trafficking. SNXs play a role in maintaining the integrity of the proteome which is essential for regulating multiple fundamental processes such as cell cycle progression, transcription, metabolism, and stress response. To tightly regulate these processes proteins must be expressed and degraded in the correct location and at the correct time. The cell employs several proteolysis mechanisms to ensure that proteins are selectively degraded at the appropriate spatiotemporal conditions. SNXs play a role in ubiquitin-mediated protein homeostasis at multiple levels including cargo localization, recycling, degradation, and function. In this review, we will discuss the role of SNXs in three different protein homeostasis systems: endocytosis lysosomal, the ubiquitin-proteasomal, and the autophagy-lysosomal system. The highly conserved nature of this protein family by beginning with the early research on SNXs and protein trafficking in yeast and lead into their important roles in mammalian systems.
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Article in Press
G Model YSCDB-1564; No. of Pages 10 ARTICLE IN PRESS Seminars in Cell & Developmental Biology xxx (2014) xxx–xxx Contents lists available at ScienceDirect Seminars in Cell & Developmental Biology j ournal homepage: www.elsevier.com/locate/semcdb Review The role of the cytoskeleton and molecular motors in endosomal dynamics 1 1 ∗ ∗∗ Elizabeth Granger , Gavin McNee , Victoria Allan , Philip Woodman Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK a r t i c l e i n f o a b s t r a c t Article history: The endocytic pathway is essential for processes that define how cells interact with their environment, Available online xxx including receptor signalling, cell adhesion and migration, pathogen entry, membrane protein turnover and nutrient uptake. The spatial organisation of endocytic trafficking requires motor proteins that tether Keywords: membranes or transport them along the actin and microtubule cytoskeletons. Microtubules, actin fila- Endosome ments and motor proteins also provide force to deform and assist in the scission of membranes, thereby Lysosome facilitating endosomal sorting and the generation of transport intermediates. Recycling © 2014 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license Dynein Kinesin (http://creativecommons.org/licenses/by/3.0/). Myosin Contents 1. The endosomal system: an overview. 00 2. Motor proteins . 00 3. Uptake from the plasma membrane: the role of actin . 00 4. Transport away from the cell cortex . 00 5. Endosome motility . 00 5.1. The molecular basis for dynein-driven endosome/lysosome motility . 00 5.2. The molecular basis for outward microtubule-based endosome/lysosome motility .
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