Kops Research 2015 Tromer

Total Page:16

File Type:pdf, Size:1020Kb

Kops Research 2015 Tromer GBE Widespread Recurrent Patterns of Rapid Repeat Evolution in the Kinetochore Scaffold KNL1 Eelco Tromer1,2,3, Berend Snel3,*,y, and Geert J.P.L. Kops1,2,4,*,y 1Molecular Cancer Research, University Medical Center Utrecht, The Netherlands 2Center for Molecular Medicine, University Medical Center Utrecht, The Netherlands 3Theoretical Biology and Bioinformatics, Department of Biology, Faculty of Science, Utrecht University, The Netherlands 4Cancer Genomics Netherlands, University Medical Center Utrecht, The Netherlands *Corresponding author: E-mail: [email protected]; [email protected]. yThese authors contributed equally to this work. Accepted: July 22, 2015 Abstract The outer kinetochore protein scaffold KNL1 is essential for error-free chromosome segregation during mitosis and meiosis. A critical feature of KNL1 is an array of repeats containing MELT-like motifs. When phosphorylated, these motifs form docking sites for the BUB1–BUB3 dimer that regulates chromosome biorientation and the spindle assembly checkpoint. KNL1 homologs are strikingly different in both the amount and sequence of repeats they harbor. We used sensitive repeat discovery and evolutionary reconstruc- tion to show that the KNL1 repeat arrays have undergone extensive, often species-specific array reorganization through iterative cycles of higher order multiplication in conjunction with rapid sequence diversification. The number of repeats per array ranges from none in flowering plants up to approximately 35–40 in drosophilids. Remarkably, closely related drosophilid species have indepen- dently expanded specific repeats, indicating near complete array replacement after only approximately 25–40 Myr of evolution. We further show that repeat sequences were altered by the parallel emergence/loss of various short linear motifs, including phosphosites, which supplement the MELT-like motif, signifying modular repeat evolution. These observations point to widespread recurrent episodes of concerted KNL1 repeat evolution in all eukaryotic supergroups. We discuss our findings in the light of the conserved function of KNL1 repeats in localizing the BUB1–BUB3 dimer and its role in chromosome segregation. Key words: KNL1, kinetochore, repeat evolution, BUB1, mitosis. Introduction microtubule-binding interface of kinetochores (Foley and Mitotic chromosome segregation in eukaryotes involves the Kapoor 2012; Sacristan and Kops 2015). The focal point of capture and stable attachment of the plus ends of spindle this interplay is KNL1/CASC5/AF15q14/Blinkin (hereafter re- microtubules by all chromosomes in a manner that connects ferred to as KNL1), a largely disordered protein that recruits sister chromatids to opposing spindle poles. Large multiprotein various mitotic regulators to the kinetochore and is able to assemblies on centromeric DNA, known as kinetochores, fa- directly interact with microtubules (Welburn et al. 2011; cilitate such chromosome–spindle interactions (Santaguida Caldas and DeLuca 2014)(fig. 1). and Musacchio 2009). In addition to providing a link between Critical for KNL1’s role in ensuring high fidelity chromo- DNA and the spindle, kinetochores are the signaling hubs for some segregation is the recruitment of the paralogs BUBR1 the spindle assembly checkpoint (SAC) and the target of and BUB1 (BUBs) to the outer kinetochore. Both BUBR1 and attachment-error correction mechanisms (Santaguida and BUB1 are bifunctional proteins, being involved in the SAC as Musacchio 2009; London and Biggins 2014; Sacristan and well as in regulating stability of kinetochore–microtubule in- Kops 2015). The interplay between microtubule attachment, teractions (Bolanos-Garcia and Blundell 2011). Their roles in error-correction, and SAC signaling is centered on the KMN these processes are, however, distinct. BUBR1 is a pseudoki- network (KNL1-C, MIS12-C, and NDC80-C), an outer- nase (Suijkerbuijk et al. 2012) that is a component of a diffus- kinetochore multiprotein complex that forms the ible anaphase inhibitor (Tang et al. 2001; Sudakin et al. 2001; ß The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non- commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact [email protected] Genome Biol. Evol. 7(8):2383–2393. doi:10.1093/gbe/evv140 Advance Access publication August 8, 2015 2383 Downloaded from https://academic.oup.com/gbe/article-abstract/7/8/2383/557758 by University Library Utrecht user on 03 December 2017 Tromer et al. GBE Chao et al. 2012; Han et al. 2013) and regulates stability of kinetochore, which raises the question of the significance of kinetochore–microtubule attachments by localizing the phos- the other 14 repeats. In addition, although pivotal for proper phatase PP2A-B56 to kinetochores (Suijkerbuijk et al. 2012; error-correction and SAC function, preliminary analyses hinted Kruse et al. 2013; Xu et al. 2013). BUB1 regulates error-cor- at a high degree of variation in KNL1 repeat evolution (Vleugel rection by localizing Aurora B kinase to the inner centromere et al. 2012, 2013). Although MELT-like motifs were at the through the phosphorylation of T120 on the Histone 2A tail core of repeat units of most KNL1 orthologs we analyzed, (Kawashima et al. 2010; Yamagishi et al. 2010) and likely by the remainder of the repeat sequences diverged greatly, and localizing BUBR1/PP2A to kinetochores (Johnson et al. 2004; instances were observed where even a MELT-like motif was Klebig et al. 2009; Overlack et al. 2015), yet its role in the SAC indiscernible. is less well identified (Bolanos-Garcia and Blundell 2011). We performed phylogenetic analyses to reconstruct KNL1 These two BUBs directly interact through their respective repeat evolution with the aim to understand its highly TPR (tetratricopeptide repeat) domains with two different KI divergent patterns and the possible implications for BUB motifs in the N-terminus of KNL1 (Bolanos-Garcia et al. 2011; kinetochore recruitment and chromosome segregation in Kiyomitsu et al. 2011; Krenn et al. 2012). These motifs are, eukaryotes. however, not conserved beyond vertebrates and are not es- sential for BUB kinetochore binding in human cells (Vleugel Materials and Methods et al. 2013; Krenn et al. 2014). Rather, the main BUB-recruit- ment site on KNL1 is an array of multiple so-called MELT re- Sequences peats (Met-Glu-Leu-Thr). When phosphorylated by the mitotic Classical homology searches using BLAST (Basic Local kinase MPS1, they form phospho-docking sites for BUB3/ Alignment Search Tool) failed to detect sufficient homology BUB1 dimers, hence directly ensuring localization of BUB1 for KNL1 genes. We therefore performed iterated sensitive and indirectly of BUBR1 to kinetochores (London et al. homology searches with HMMer (Eddy 2011; Finn et al. 2012; Shepperd et al. 2012; Primorac et al. 2013). 2011), using a permissive E value and bitscore cut-off to in- We and others recently reported that the MELT repeats of clude diverged homologs. Given that we detected a single human KNL1 are part of larger repeated units that contain homolog per genome we considered them orthologs. We in- (besides a central MELT-like motif) at least two other motifs cluded orthologs based on the presence of a N-terminal PP1- required for function: A T motif (Txx ;where denotes recruitment motifs (SILK/RVSF), MELT-like repeats, conserved aromatic residues), and a second phospho motif (SHT) C- regions in the C-terminus including a recently discovered RWD terminal to the MELT motif (Vleugel et al. 2013, 2015; domain (Petrovic et al. 2014), and a C-terminal coiled-coil Krenn et al. 2014). Human KNL1 has approximately 20 of region. Incompletely predicted genes were searched against these larger repeats. We showed that only six repeats are whole-genome shotgun contigs (wgs; http://www.ncbi.nlm. capable of recruiting detectable BUB proteins to the nih.gov/genbank/wgs) using tBLASTn. Significant hits were FIG.1.—KNL1 is a hub for signaling at the kinetochore–microtubule interface. Schematic representation of the domain/motif architecture of human KNL1. Phospho motifs (MELTs) in the disordered middle region of KNL1 function as binding sites for various factors involved in SAC activation and error- correction (BUB3–BUB1/BUBR1). KI1 and KI2 increase the affinity of the BUB proteins for repeat 1. In addition this region harbors a basic patch involvedin microtubule binding, as well as SILK/RVSF motifs for recruitment of PP1 phosphatase. PP1 can dephosphorylate the phospho-MELT motifs. The C-terminal region contains a tandem RWD (RING-WD40-DEAD) domain that localizes KNL1 to kinetochores and a coiled-coil that interacts with Zwint-1, a factor involved in recruiting the dynein adaptor RZZ–Spindly complex to kinetochores. 2384 Genome Biol. Evol. 7(8):2383–2393. doi:10.1093/gbe/evv140 Advance Access publication August 8, 2015 Downloaded from https://academic.oup.com/gbe/article-abstract/7/8/2383/557758 by University Library Utrecht user on 03 December 2017 Evolution of KNL1 repeats GBE manually predicted using AUGUST (Stanke et al. 2006)and Repeat arrays in KNL1 orthologs display rapid consensus GENESCAN (Burge and Karlin 1997). For the sequences that sequence evolution and extensive number changes we used in this study,
Recommended publications
  • PARSANA-DISSERTATION-2020.Pdf
    DECIPHERING TRANSCRIPTIONAL PATTERNS OF GENE REGULATION: A COMPUTATIONAL APPROACH by Princy Parsana A dissertation submitted to The Johns Hopkins University in conformity with the requirements for the degree of Doctor of Philosophy Baltimore, Maryland July, 2020 © 2020 Princy Parsana All rights reserved Abstract With rapid advancements in sequencing technology, we now have the ability to sequence the entire human genome, and to quantify expression of tens of thousands of genes from hundreds of individuals. This provides an extraordinary opportunity to learn phenotype relevant genomic patterns that can improve our understanding of molecular and cellular processes underlying a trait. The high dimensional nature of genomic data presents a range of computational and statistical challenges. This dissertation presents a compilation of projects that were driven by the motivation to efficiently capture gene regulatory patterns in the human transcriptome, while addressing statistical and computational challenges that accompany this data. We attempt to address two major difficulties in this domain: a) artifacts and noise in transcriptomic data, andb) limited statistical power. First, we present our work on investigating the effect of artifactual variation in gene expression data and its impact on trans-eQTL discovery. Here we performed an in-depth analysis of diverse pre-recorded covariates and latent confounders to understand their contribution to heterogeneity in gene expression measurements. Next, we discovered 673 trans-eQTLs across 16 human tissues using v6 data from the Genotype Tissue Expression (GTEx) project. Finally, we characterized two trait-associated trans-eQTLs; one in Skeletal Muscle and another in Thyroid. Second, we present a principal component based residualization method to correct gene expression measurements prior to reconstruction of co-expression networks.
    [Show full text]
  • The MIS12 Complex Is a Protein Interaction Hub for Outer Kinetochore Assembly
    JCB: Article The MIS12 complex is a protein interaction hub for outer kinetochore assembly Arsen Petrovic,1 Sebastiano Pasqualato,1 Prakash Dube,3 Veronica Krenn,1 Stefano Santaguida,1 Davide Cittaro,4 Silvia Monzani,1 Lucia Massimiliano,1 Jenny Keller,1 Aldo Tarricone,1 Alessio Maiolica,1 Holger Stark,3 and Andrea Musacchio1,2 1Department of Experimental Oncology, European Institute of Oncology (IEO) and 2Research Unit of the Italian Institute of Technology, Italian Foundation for Cancer Research Institute of Molecular Oncology–IEO Campus, I-20139 Milan, Italy 33D Electron Cryomicroscopy Group, Max Planck Institute for Biophysical Chemistry, and Göttingen Center for Microbiology, University of Göttingen, 37077 Göttingen, Germany 4Consortium for Genomic Technologies, I-20139 Milan, Italy inetochores are nucleoprotein assemblies responsi­ axis of 22 nm. Through biochemical analysis, cross- ble for the attachment of chromosomes to spindle linking–based methods, and negative-stain electron mi­ K microtubules during mitosis. The KMN network, croscopy, we investigated the reciprocal organization of a crucial constituent of the outer kinetochore, creates an the subunits of the MIS12 complex and their contacts with interface that connects microtubules to centromeric chro­ the rest of the KMN network. A highlight of our findings matin. The NDC80, MIS12, and KNL1 complexes form the is the identification of the NSL1 subunit as a scaffold core of the KMN network. We recently reported the struc­ supporting interactions of the MIS12 complex with the tural organization of the human NDC80 complex. In this NDC80 and KNL1 complexes. Our analysis has important study, we extend our analysis to the human MIS12 com­ implications for understanding kinetochore organization in plex and show that it has an elongated structure with a long different organisms.
    [Show full text]
  • Congenital Microcephaly
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Sussex Research Online American Journal of Medical Genetics Part C (Seminars in Medical Genetics) ARTICLE Congenital Microcephaly DIANA ALCANTARA AND MARK O'DRISCOLL* The underlying etiologies of genetic congenital microcephaly are complex and multifactorial. Recently, with the exponential growth in the identification and characterization of novel genetic causes of congenital microcephaly, there has been a consolidation and emergence of certain themes concerning underlying pathomechanisms. These include abnormal mitotic microtubule spindle structure, numerical and structural abnormalities of the centrosome, altered cilia function, impaired DNA repair, DNA Damage Response signaling and DNA replication, along with attenuated cell cycle checkpoint proficiency. Many of these processes are highly interconnected. Interestingly, a defect in a gene whose encoded protein has a canonical function in one of these processes can often have multiple impacts at the cellular level involving several of these pathways. Here, we overview the key pathomechanistic themes underlying profound congenital microcephaly, and emphasize their interconnected nature. © 2014 Wiley Periodicals, Inc. KEY WORDS: cell division; mitosis; DNA replication; cilia How to cite this article: Alcantara D, O'Driscoll M. 2014. Congenital microcephaly. Am J Med Genet Part C Semin Med Genet 9999:1–16. INTRODUCTION mid‐gestation although glial cell division formation of the various cortical layers. and consequent brain volume enlarge- Furthermore, differentiating and devel- Congenital microcephaly, an occipital‐ ment does continue after birth [Spalding oping neurons must migrate to their frontal circumference of equal to or less et al., 2005]. Impaired neurogenesis is defined locations to construct the com- than 2–3 standard deviations below the therefore most obviously reflected clini- plex architecture and laminar layered age‐related population mean, denotes cally as congenital microcephaly.
    [Show full text]
  • Real-Time Dynamics of Plasmodium NDC80 Reveals Unusual Modes of Chromosome Segregation During Parasite Proliferation Mohammad Zeeshan1,*, Rajan Pandey1,*, David J
    © 2020. Published by The Company of Biologists Ltd | Journal of Cell Science (2021) 134, jcs245753. doi:10.1242/jcs.245753 RESEARCH ARTICLE SPECIAL ISSUE: CELL BIOLOGY OF HOST–PATHOGEN INTERACTIONS Real-time dynamics of Plasmodium NDC80 reveals unusual modes of chromosome segregation during parasite proliferation Mohammad Zeeshan1,*, Rajan Pandey1,*, David J. P. Ferguson2,3, Eelco C. Tromer4, Robert Markus1, Steven Abel5, Declan Brady1, Emilie Daniel1, Rebecca Limenitakis6, Andrew R. Bottrill7, Karine G. Le Roch5, Anthony A. Holder8, Ross F. Waller4, David S. Guttery9 and Rita Tewari1,‡ ABSTRACT eukaryotic organisms to proliferate, propagate and survive. During Eukaryotic cell proliferation requires chromosome replication and these processes, microtubular spindles form to facilitate an equal precise segregation to ensure daughter cells have identical genomic segregation of duplicated chromosomes to the spindle poles. copies. Species of the genus Plasmodium, the causative agents of Chromosome attachment to spindle microtubules (MTs) is malaria, display remarkable aspects of nuclear division throughout their mediated by kinetochores, which are large multiprotein complexes life cycle to meet some peculiar and unique challenges to DNA assembled on centromeres located at the constriction point of sister replication and chromosome segregation. The parasite undergoes chromatids (Cheeseman, 2014; McKinley and Cheeseman, 2016; atypical endomitosis and endoreduplication with an intact nuclear Musacchio and Desai, 2017; Vader and Musacchio, 2017). Each membrane and intranuclear mitotic spindle. To understand these diverse sister chromatid has its own kinetochore, oriented to facilitate modes of Plasmodium cell division, we have studied the behaviour movement to opposite poles of the spindle apparatus. During and composition of the outer kinetochore NDC80 complex, a key part of anaphase, the spindle elongates and the sister chromatids separate, the mitotic apparatus that attaches the centromere of chromosomes to resulting in segregation of the two genomes during telophase.
    [Show full text]
  • Supplemental Information
    Supplemental information Dissection of the genomic structure of the miR-183/96/182 gene. Previously, we showed that the miR-183/96/182 cluster is an intergenic miRNA cluster, located in a ~60-kb interval between the genes encoding nuclear respiratory factor-1 (Nrf1) and ubiquitin-conjugating enzyme E2H (Ube2h) on mouse chr6qA3.3 (1). To start to uncover the genomic structure of the miR- 183/96/182 gene, we first studied genomic features around miR-183/96/182 in the UCSC genome browser (http://genome.UCSC.edu/), and identified two CpG islands 3.4-6.5 kb 5’ of pre-miR-183, the most 5’ miRNA of the cluster (Fig. 1A; Fig. S1 and Seq. S1). A cDNA clone, AK044220, located at 3.2-4.6 kb 5’ to pre-miR-183, encompasses the second CpG island (Fig. 1A; Fig. S1). We hypothesized that this cDNA clone was derived from 5’ exon(s) of the primary transcript of the miR-183/96/182 gene, as CpG islands are often associated with promoters (2). Supporting this hypothesis, multiple expressed sequences detected by gene-trap clones, including clone D016D06 (3, 4), were co-localized with the cDNA clone AK044220 (Fig. 1A; Fig. S1). Clone D016D06, deposited by the German GeneTrap Consortium (GGTC) (http://tikus.gsf.de) (3, 4), was derived from insertion of a retroviral construct, rFlpROSAβgeo in 129S2 ES cells (Fig. 1A and C). The rFlpROSAβgeo construct carries a promoterless reporter gene, the β−geo cassette - an in-frame fusion of the β-galactosidase and neomycin resistance (Neor) gene (5), with a splicing acceptor (SA) immediately upstream, and a polyA signal downstream of the β−geo cassette (Fig.
    [Show full text]
  • Molecular Genetics of Microcephaly Primary Hereditary: an Overview
    brain sciences Review Molecular Genetics of Microcephaly Primary Hereditary: An Overview Nikistratos Siskos † , Electra Stylianopoulou †, Georgios Skavdis and Maria E. Grigoriou * Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece; [email protected] (N.S.); [email protected] (E.S.); [email protected] (G.S.) * Correspondence: [email protected] † Equal contribution. Abstract: MicroCephaly Primary Hereditary (MCPH) is a rare congenital neurodevelopmental disorder characterized by a significant reduction of the occipitofrontal head circumference and mild to moderate mental disability. Patients have small brains, though with overall normal architecture; therefore, studying MCPH can reveal not only the pathological mechanisms leading to this condition, but also the mechanisms operating during normal development. MCPH is genetically heterogeneous, with 27 genes listed so far in the Online Mendelian Inheritance in Man (OMIM) database. In this review, we discuss the role of MCPH proteins and delineate the molecular mechanisms and common pathways in which they participate. Keywords: microcephaly; MCPH; MCPH1–MCPH27; molecular genetics; cell cycle 1. Introduction Citation: Siskos, N.; Stylianopoulou, Microcephaly, from the Greek word µικρoκεϕαλi´α (mikrokephalia), meaning small E.; Skavdis, G.; Grigoriou, M.E. head, is a term used to describe a cranium with reduction of the occipitofrontal head circum- Molecular Genetics of Microcephaly ference equal, or more that teo standard deviations
    [Show full text]
  • Discovery of Novel Putative Tumor Suppressors from CRISPR Screens Reveals Rewired 2 Lipid Metabolism in AML Cells 3 4 W
    bioRxiv preprint doi: https://doi.org/10.1101/2020.10.08.332023; this version posted August 20, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Discovery of novel putative tumor suppressors from CRISPR screens reveals rewired 2 lipid metabolism in AML cells 3 4 W. Frank Lenoir1,2, Micaela Morgado2, Peter C DeWeirdt3, Megan McLaughlin1,2, Audrey L 5 Griffith3, Annabel K Sangree3, Marissa N Feeley3, Nazanin Esmaeili Anvar1,2, Eiru Kim2, Lori L 6 Bertolet2, Medina Colic1,2, Merve Dede1,2, John G Doench3, Traver Hart2,4,* 7 8 9 1 - The University of Texas MD Anderson Cancer Center UTHealth Graduate School of 10 Biomedical Sciences; The University of Texas MD Anderson Cancer Center, Houston, TX 11 12 2 - Department of Bioinformatics and Computational Biology, The University of Texas MD 13 Anderson Cancer Center, Houston, TX, USA 14 15 3 - Genetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA, USA 16 17 4 - Department of Cancer Biology, The University of Texas MD Anderson Cancer Center, 18 Houston, TX, USA 19 20 21 22 23 * - Corresponding author: [email protected] 24 25 bioRxiv preprint doi: https://doi.org/10.1101/2020.10.08.332023; this version posted August 20, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.
    [Show full text]
  • Bub1 Positions Mad1 Close to KNL1 MELT Repeats to Promote Checkpoint Signalling
    ARTICLE Received 14 Dec 2016 | Accepted 3 May 2017 | Published 12 June 2017 DOI: 10.1038/ncomms15822 OPEN Bub1 positions Mad1 close to KNL1 MELT repeats to promote checkpoint signalling Gang Zhang1, Thomas Kruse1, Blanca Lo´pez-Me´ndez1, Kathrine Beck Sylvestersen1, Dimitriya H. Garvanska1, Simone Schopper1, Michael Lund Nielsen1 & Jakob Nilsson1 Proper segregation of chromosomes depends on a functional spindle assembly checkpoint (SAC) and requires kinetochore localization of the Bub1 and Mad1/Mad2 checkpoint proteins. Several aspects of Mad1/Mad2 kinetochore recruitment in human cells are unclear and in particular the underlying direct interactions. Here we show that conserved domain 1 (CD1) in human Bub1 binds directly to Mad1 and a phosphorylation site exists in CD1 that stimulates Mad1 binding and SAC signalling. Importantly, fusion of minimal kinetochore-targeting Bub1 fragments to Mad1 bypasses the need for CD1, revealing that the main function of Bub1 is to position Mad1 close to KNL1 MELTrepeats. Furthermore, we identify residues in Mad1 that are critical for Mad1 functionality, but not Bub1 binding, arguing for a direct role of Mad1 in the checkpoint. This work dissects functionally relevant molecular interactions required for spindle assembly checkpoint signalling at kinetochores in human cells. 1 The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark. Correspondence and requests for materials should be addressed to G.Z.
    [Show full text]
  • Differential Expression of ZWINT in Cancers of the Breast
    Differential expression of ZW10 interacting kinetochore protein in cancers of the breast. Shahan Mamoor, MS1 [email protected] East Islip, NY 11730 Breast cancer affects women at relatively high frequency1. We mined published microarray datasets2,3 to determine in an unbiased fashion and at the systems level genes most differentially expressed in the primary tumors of patients with breast cancer. We report here significant differential expression of the gene encoding the ZW10 interacting kinetochore protein, ZWINT, when comparing primary tumors of the breast to the tissue of origin, the normal breast. ZWINT was also differentially expressed in the tumor cells of patients with triple negative breast cancer. ZWINT mRNA was present at significantly higher quantities in tumors of the breast as compared to normal breast tissue. Analysis of human survival data revealed that expression of ZWINT in primary tumors of the breast was correlated with overall survival in patients with basal and luminal A subtype cancer, but in a contrary manner, demonstrating a complex relationship between correlation of primary tumor expression with overall survival based on molecular subtype. ZWINT may be of relevance to initiation, maintenance or progression of cancers of the female breast. Keywords: breast cancer, ZWINT, ZW10 interacting kinetochore protein, systems biology of breast cancer, targeted therapeutics in breast cancer. 1 Invasive breast cancer is diagnosed in over a quarter of a million women in the United States each year1 and in 2018, breast cancer was the leading cause of cancer death in women worldwide4. While patients with localized breast cancer are provided a 99% 5-year survival rate, patients with regional breast cancer, cancer that has spread to lymph nodes or nearby structures, are provided an 86% 5-year survival rate5,6.
    [Show full text]
  • The Genome of Schmidtea Mediterranea and the Evolution Of
    OPEN ArtICLE doi:10.1038/nature25473 The genome of Schmidtea mediterranea and the evolution of core cellular mechanisms Markus Alexander Grohme1*, Siegfried Schloissnig2*, Andrei Rozanski1, Martin Pippel2, George Robert Young3, Sylke Winkler1, Holger Brandl1, Ian Henry1, Andreas Dahl4, Sean Powell2, Michael Hiller1,5, Eugene Myers1 & Jochen Christian Rink1 The planarian Schmidtea mediterranea is an important model for stem cell research and regeneration, but adequate genome resources for this species have been lacking. Here we report a highly contiguous genome assembly of S. mediterranea, using long-read sequencing and a de novo assembler (MARVEL) enhanced for low-complexity reads. The S. mediterranea genome is highly polymorphic and repetitive, and harbours a novel class of giant retroelements. Furthermore, the genome assembly lacks a number of highly conserved genes, including critical components of the mitotic spindle assembly checkpoint, but planarians maintain checkpoint function. Our genome assembly provides a key model system resource that will be useful for studying regeneration and the evolutionary plasticity of core cell biological mechanisms. Rapid regeneration from tiny pieces of tissue makes planarians a prime De novo long read assembly of the planarian genome model system for regeneration. Abundant adult pluripotent stem cells, In preparation for genome sequencing, we inbred the sexual strain termed neoblasts, power regeneration and the continuous turnover of S. mediterranea (Fig. 1a) for more than 17 successive sib- mating of all cell types1–3, and transplantation of a single neoblast can rescue generations in the hope of decreasing heterozygosity. We also developed a lethally irradiated animal4. Planarians therefore also constitute a a new DNA isolation protocol that meets the purity and high molecular prime model system for stem cell pluripotency and its evolutionary weight requirements of PacBio long-read sequencing12 (Extended Data underpinnings5.
    [Show full text]
  • Review Article Molecular and Cellular Basis of Autosomal Recessive Primary Microcephaly
    Hindawi Publishing Corporation BioMed Research International Volume 2014, Article ID 547986, 13 pages http://dx.doi.org/10.1155/2014/547986 Review Article Molecular and Cellular Basis of Autosomal Recessive Primary Microcephaly Marine Barbelanne1,2 and William Y. Tsang1,2,3 1 Institut de Recherches Cliniques de Montreal,´ 110 avenue des Pins Ouest, Montreal,QC,CanadaH2W1R7´ 2 FacultedeM´ edecine,´ UniversitedeMontr´ eal,´ Montreal,QC,CanadaH3C3J7´ 3 Division of Experimental Medicine, McGill University, Montreal,´ QC, Canada H3A 1A3 Correspondence should be addressed to William Y. Tsang; [email protected] Received 16 July 2014; Revised 18 September 2014; Accepted 18 September 2014; Published 8 December 2014 Academic Editor: Saulius Butenas Copyright © 2014 M. Barbelanne and W. Y. Tsang. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Autosomal recessive primary microcephaly (MCPH) is a rare hereditary neurodevelopmental disorder characterized by a marked reduction in brain size and intellectual disability. MCPH is genetically heterogeneous and can exhibit additional clinical features that overlap with related disorders including Seckel syndrome, Meier-Gorlin syndrome, and microcephalic osteodysplastic dwarfism. In this review, we discuss the key proteins mutated in MCPH. To date, MCPH-causing mutations have been identified in twelve different genes, many of which encode proteins that are involved in cell cycle regulation or are present at the centrosome, an organelle crucial for mitotic spindle assembly and cell division. We highlight recent findings on MCPH proteins with regard to their role in cell cycle progression, centrosome function, and early brain development.
    [Show full text]
  • 32-5283: Recombinant Human ZW10 Interacting Kinetochore Protein
    9853 Pacific Heights Blvd. Suite D. San Diego, CA 92121, USA Tel: 858-263-4982 Email: [email protected] 32-5283: Recombinant Human ZW10 Interacting Kinetochore Protein Alternative ZW10 Interacting Kinetochore Protein,ZWINT,ZW10 Interactor,HZwint-1,KNTC2AP,ZWINT1,Human ZW10 Name : Interacting Protein-1,ZW10 Interactor Kinetochore Protein zwint-1,Zwint-1,ZW10-Interacting Protein 1. Description Source : Escherichia Coli. ZWINT Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 253 amino acids (1-230) and having a molecular mass of 27.9 kDa (Molecular size on SDS-PAGE will appear higher).ZWINT is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. ZW10 Interacting Kinetochore Protein (ZWINT) is involved in kinetochore function. ZWINT is part of the MIS12 complex, which is essential for kinetochore formation and spindle checkpoint activity. ZWINT is localized to the cytoplasm during interphase and to kinetochores from late prophase to anaphase. ZWINT interacts with ZW10 (Zeste White 10) and regulates the association between ZW10 and kinetochores. ZWINT gene defects are linked with the pathogenesis of Roberts's syndrome, an autosomal recessive disorder characterized by growth retardation due to premature chromosome separation. Product Info Amount : 10 µg Purification : Greater than 85.0% as determined by SDS-PAGE. The ZWINT solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% Content : glycerol and 1mM DTT. Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of Storage condition : time.
    [Show full text]