Comparative Plastomics of Amaryllidaceae: Inverted Repeat
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Specific Binding of Transposase to Terminal Inverted Repeats Of
Proc. Natl. Acad. Sci. USA Vol. 84, pp. 8220-8224, December 1987 Biochemistry Specific binding of transposase to terminal inverted repeats of transposable element Tn3 (transposon/DNA binding protein/DNase I "footprinting") HITOSHI ICHIKAWA*, KAORU IKEDA*, WILLIAM L. WISHARTt, AND EIICHI OHTSUBO*t *Institute of Applied Microbiology, University of Tokyo, Bunkyo-ku, Yayoi 1-1-1, Tokyo 113, Japan; and tDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544 Communicated by Norman Davidson, July 27, 1987 ABSTRACT Tn3 transposase, which is required for trans- containing the Tn3 terminal regions when 8 mM ATP is position of Tn3, has been purified by a low-ionic-strength- present (14). precipitation method. Using a nitrocellulose filter binding In this paper, we study the interaction of Tn3 transposase, assay, we have shown that transposase binds to any restriction which has been purified by a low-ionic-strength-precipitation fragment. However, binding of the transposase to specific method, with DNA. We demonstrate that the purified fragments containing the terminal inverted repeat sequences of transposase is an ATP-independent DNA binding protein, Tn3 can be demonstrated by treatment of transposase-DNA which has interesting properties that may be involved in the complexes with heparin, which effectively removes the actual transposition event of Tn3. transposase bound to the other nonspecific fragments at pH 5-6. DNase I "footprinting" analysis showed that the MATERIALS AND METHODS transposase protects an inner 25-base-pair region of the 38- base-pair terminal inverted repeat sequence of Tn3. This Bacterial Strains and Plasmids. The bacterial strains used protection is not dependent on pH. -
Liliaceae S.L. (Lily Family)
Liliaceae s.l. (Lily family) Photo: Ben Legler Photo: Hannah Marx Photo: Hannah Marx Lilium columbianum Xerophyllum tenax Trillium ovatum Liliaceae s.l. (Lily family) Photo: Yaowu Yuan Fritillaria lanceolata Ref.1 Textbook DVD KRR&DLN Erythronium americanum Allium vineale Liliaceae s.l. (Lily family) Herbs; Ref.2 Stems often modified as underground rhizomes, corms, or bulbs; Flowers actinomorphic; 3 sepals and 3 petals or 6 tepals, 6 stamens, 3 carpels, ovary superior (or inferior). Tulipa gesneriana Liliaceae s.l. (Lily family) “Liliaceae” s.l. (sensu lato: “in the broad sense”) - Lily family; 288 genera/4950 species, including Lilium, Allium, Trillium, Tulipa; This family is treated in a very broad sense in this class, as in the Flora of the Pacific Northwest. The “Liliaceae” s.l. taught in this class is not monophyletic. It is apparent now that the family should be treated in a narrower sense and some of the members should form their own families. Judd et al. recognize 15+ families: Agavaceae, Alliaceae, Amarylidaceae, Asparagaceae, Asphodelaceae, Colchicaceae, Dracaenaceae (Nolinaceae), Hyacinthaceae, Liliaceae, Melanthiaceae, Ruscaceae, Smilacaceae, Themidaceae, Trilliaceae, Uvulariaceae and more!!! (see web reading “Consider the Lilies”) Iridaceae (Iris family) Photo: Hannah Marx Photo: Hannah Marx Iris pseudacorus Iridaceae (Iris family) Photo: Yaowu Yuan Photo: Yaowu Yuan Sisyrinchium douglasii Sisyrinchium sp. Iridaceae (Iris family) Iridaceae - 78 genera/1750 species, Including Iris, Gladiolus, Sisyrinchium. Herbs, aquatic or terrestrial; Underground stems as rhizomes, bulbs, or corms; Leaves alternate, 2-ranked and equitant Ref.3 (oriented edgewise to the stem; Gladiolus italicus Flowers actinomorphic or zygomorphic; 3 sepals and 3 petals or 6 tepals; Stamens 3; Ovary of 3 fused carpels, inferior. -
Summary of Offerings in the PBS Bulb Exchange, Dec 2012- Nov 2019
Summary of offerings in the PBS Bulb Exchange, Dec 2012- Nov 2019 3841 Number of items in BX 301 thru BX 463 1815 Number of unique text strings used as taxa 990 Taxa offered as bulbs 1056 Taxa offered as seeds 308 Number of genera This does not include the SXs. Top 20 Most Oft Listed: BULBS Times listed SEEDS Times listed Oxalis obtusa 53 Zephyranthes primulina 20 Oxalis flava 36 Rhodophiala bifida 14 Oxalis hirta 25 Habranthus tubispathus 13 Oxalis bowiei 22 Moraea villosa 13 Ferraria crispa 20 Veltheimia bracteata 13 Oxalis sp. 20 Clivia miniata 12 Oxalis purpurea 18 Zephyranthes drummondii 12 Lachenalia mutabilis 17 Zephyranthes reginae 11 Moraea sp. 17 Amaryllis belladonna 10 Amaryllis belladonna 14 Calochortus venustus 10 Oxalis luteola 14 Zephyranthes fosteri 10 Albuca sp. 13 Calochortus luteus 9 Moraea villosa 13 Crinum bulbispermum 9 Oxalis caprina 13 Habranthus robustus 9 Oxalis imbricata 12 Haemanthus albiflos 9 Oxalis namaquana 12 Nerine bowdenii 9 Oxalis engleriana 11 Cyclamen graecum 8 Oxalis melanosticta 'Ken Aslet'11 Fritillaria affinis 8 Moraea ciliata 10 Habranthus brachyandrus 8 Oxalis commutata 10 Zephyranthes 'Pink Beauty' 8 Summary of offerings in the PBS Bulb Exchange, Dec 2012- Nov 2019 Most taxa specify to species level. 34 taxa were listed as Genus sp. for bulbs 23 taxa were listed as Genus sp. for seeds 141 taxa were listed with quoted 'Variety' Top 20 Most often listed Genera BULBS SEEDS Genus N items BXs Genus N items BXs Oxalis 450 64 Zephyranthes 202 35 Lachenalia 125 47 Calochortus 94 15 Moraea 99 31 Moraea -
Asphodelus Fistulosus (Asphodelaceae, Asphodeloideae), a New Naturalised Alien Species from the West Coast of South Africa ⁎ J.S
Available online at www.sciencedirect.com South African Journal of Botany 79 (2012) 48–50 www.elsevier.com/locate/sajb Research note Asphodelus fistulosus (Asphodelaceae, Asphodeloideae), a new naturalised alien species from the West Coast of South Africa ⁎ J.S. Boatwright Compton Herbarium, South African National Biodiversity Institute, Private Bag X7, Claremont 7735, South Africa Department of Botany and Plant Biotechnology, University of Johannesburg, P.O. Box 524, Auckland Park 2006, Johannesburg, South Africa Received 4 November 2011; received in revised form 18 November 2011; accepted 21 November 2011 Abstract Asphodelus fistulosus or onionweed is recorded in South Africa for the first time and is the first record of an invasive member of the Asphodelaceae in the country. Only two populations of this plant have been observed, both along disturbed roadsides on the West Coast of South Africa. The extent and invasive potential of this infestation in the country is still limited but the species is known to be an aggressive invader in other parts of the world. © 2011 SAAB. Published by Elsevier B.V. All rights reserved. Keywords: Asphodelaceae; Asphodelus; Invasive species 1. Introduction flowers (Patterson, 1996). This paper reports on the presence of this species in South Africa. A population of A. fistulosus was The genus Asphodelus L. comprises 16 species distributed in first observed in the early 1990's by Drs John Manning and Eurasia and the Mediterranean (Días Lifante and Valdés, 1996). Peter Goldblatt during field work for their Wild Flower Guide It is superficially similar to the largely southern African to the West Coast (Manning and Goldblatt, 1996). -
Phylogenetic Reconstruction of the Evolution of Stylar Polymorphisms in Narcissus (Amaryllidaceae)1
American Journal of Botany 91(7): 1007±1021. 2004. INVITED SPECIAL PAPER PHYLOGENETIC RECONSTRUCTION OF THE EVOLUTION OF STYLAR POLYMORPHISMS IN NARCISSUS (AMARYLLIDACEAE)1 SEAN W. G RAHAM2,4 AND SPENCER C. H. BARRETT3 2UBC Botanical Garden and Centre for Plant Research, 6804 SW Marine Drive, The University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4; and 3Department of Botany, University of Toronto, 25 Willcocks Street, Toronto, Ontario, Canada M5S 3B2 We investigated the origin of stylar polymorphisms in Narcissus, which possesses a remarkable range of stylar conditions and diverse types of ¯oral morphology and pollination biology. Reconstruction of evolutionary change was complicated by incomplete resolution of trees inferred from two rapidly evolving chloroplast regions, but we bracketed reconstructions expected on the fully resolved plastid- based tree by considering all possible resolutions of polytomies on the shortest trees. Stigma-height dimorphism likely arose on several occasions in Narcissus and persisted across multiple speciation events. As proposed in published models, this rare type of stylar polymorphism is ancestral to distyly. While there is no evidence in Narcissus that dimorphism preceded tristyly, a rapid transition between them may explain the lack of a phylogenetic footprint for this evolutionary sequence. The single instances of distyly and tristyly in Narcissus albimarginatus and N. triandrus, respectively, are clearly not homologous, an evolutionary convergence unique to Amaryllidaceae. Floral morphology was likely an important trigger for the evolution of stylar polymorphisms: Concentrated-changes tests indicate that a long, narrow ¯oral tube may have been associated with the emergence of stigma-height dimorphism and that this type of tube, in combination with a deep corona, likely promoted, or at least was associated with, the parallel origins of heterostyly. -
Complete Article
The EMBO Journal Vol. I No. 12 pp. 1539-1544, 1982 Long terminal repeat-like elements flank a human immunoglobulin epsilon pseudogene that lacks introns Shintaro Ueda', Sumiko Nakai, Yasuyoshi Nishida, lack the entire IVS have been found in the gene families of the Hiroshi Hisajima, and Tasuku Honjo* mouse a-globin (Nishioka et al., 1980; Vanin et al., 1980), the lambda chain (Hollis et al., 1982), Department of Genetics, Osaka University Medical School, Osaka 530, human immunoglobulin Japan and the human ,B-tubulin (Wilde et al., 1982a, 1982b). The mouse a-globin processed gene is flanked by long terminal Communicated by K.Rajewsky Received on 30 September 1982 repeats (LTRs) of retrovirus-like intracisternal A particles on both sides, although their orientation is opposite to each There are at least three immunoglobulin epsilon genes (C,1, other (Lueders et al., 1982). The human processed genes CE2, and CE) in the human genome. The nucleotide sequences described above have poly(A)-like tails -20 bases 3' to the of the expressed epsilon gene (CE,) and one (CE) of the two putative poly(A) addition signal and are flanked by direct epsilon pseudogenes were compared. The results show that repeats of several bases on both sides (Hollis et al., 1982; the CE3 gene lacks the three intervening sequences entirely and Wilde et al., 1982a, 1982b). Such direct repeats, which were has a 31-base A-rich sequence 16 bases 3' to the putative also found in human small nuclear RNA pseudogenes poly(A) addition signal, indicating that the CE3 gene is a pro- (Arsdell et al., 1981), might have been formed by repair of cessed gene. -
BURIED TREASURE Summer 2019 Rannveig Wallis, Llwyn Ifan, Porthyrhyd, Carmarthen, UK
BURIED TREASURE Summer 2019 Rannveig Wallis, Llwyn Ifan, Porthyrhyd, Carmarthen, UK. SA32 8BP Email: [email protected] I am still trying unsuccessfully to retire from this enterprise. In order to reduce work, I am sowing fewer seeds and concentrating on selling excess stock which has been repotted in the current year. Some are therefore in quite small numbers. I hope that you find something of interest and order early to avoid any disappointments. Please note that my autumn seed list is included below. This means that seed is fresher and you can sow it earlier. Terms of Business: I can accept payment by either: • Cheque made out to "R Wallis" (n.b. Please do not fill in the amount but add the words “not to exceed £xx” ACROSS THE TOP); • PayPal, please include your email address with the order and wait for an invoice after I dispatch your order; • In cash (Sterling, Euro or US dollar are accepted, in this case I advise using registered mail). Please note that I can only accept orders placed before the end of August. Parcels will be dispatched at the beginning of September. If you are going to be away please let me know so that I can coordinate dispatch. I will not cash your cheque until your order is dispatched. If ordering by email, and following up by post, please ensure that you tick the box on the order form to avoid duplication. Acis autumnalis var pulchella A Moroccan version of this excellent early autumn flowerer. It is quite distinct in the fact that the pedicels and bracts are green rather than maroon as in the type variety. -
Patterns of Plant Diversity and Endemism in Namibia
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Stellenbosch University SUNScholar Repository Bothalia 36,2: 175-189(2006) Patterns of plant diversity and endemism in Namibia P. CRAVEN* and P VORSTER** Keywords: Namibia, phytogeography, plant endemism ABSTRACT Species richness, endemism and areas that are rich in both species and endemic species were assessed and mapped for Namibia. High species diversity corresponds with zones where species overlap. These are particularly obvious where there are altitudinal variations and in high-lying areas. The endemic flora o f Namibia is rich and diverse. An estimated 16% of the total plant species in Namibia are endemic to the country. Endemics are in a wide variety o f families and sixteen genera are endemic. Factors that increase the likelihood o f endemism are mountains, hot deserts, diversity o f substrates and microclimates. The distribution of plants endemic to Namibia was arranged in three different ways. Firstly, based on a grid count with the phytogeographic value of the species being equal, overall endemism was mapped. Secondly, range restricted plant species were mapped individually and those with congruent distribution patterns were combined. Thirdly, localities that are important for very range-restricted species were identified. The resulting maps of endemism and diversity were compared and found to correspond in many localities. When overall endemism is compared with overall diversity, rich localities may consist o f endemic species with wide ranges. The other methods identify important localities with their own distinctive complement of species. INTRODUCTION (1994). It was based on distributional data per magiste rial district following Merxmiiller (1966-1972), as well Species diversity was traditionally measured by count as other literature. -
Seminum 2017 Tisk
FRONT COVER: Campanula cochleariifolia Lam. SK-0-PLZEN-4260-98-20 (No. 75) Slovakia, Nízké Tatry Mts., Demänovská dolina, near hotel Repiská, calcareous rocks Photo: Jaroslav Vogeltanz INDEX SEMINUM 2017 PLZE Ň 2017 1 ZOOLOGICAL AND BOTANICAL GARDEN OF THE CITY PLZEN Pod Vinicemi 9, 301 00 Plzen Czech Republic Telephone: +420/378038301 Fax: +420/378038302 E-mail: [email protected] Area: 21,5 ha Geographical location: Latitude: 49 o 44’ N Longitude: 13 o23’ E Altitude: 330 m Annual average temperature: 7,4 oC Highest annual temperature: 40 o C Lowest annual temperature: - 27 oC Annual rainfall: 512 mm Director: Ing. Ji ří Trávní ček Curator of Botany: Ing. Tomáš Peš Seed collectors: Mgr. Václava Pešková, Radka Matulová, Petra Vonášková, Lenka Richterová, Hana Janouškovcová 2 Please notice: Complying with article 15 of the Convention on Biological Diversity (CBD, 1992), the Zoological and Botanical Garden of Plzen provides seeds and any other plant material only for botanical gardens and other scientific institutions using this materiál according to the CBD. We are part of the IPEN network (International Plant Exchange Network) and can exchange material with other IPEN-members without further bi-lateral agreements.For a list of gardens currently registered with IPEN and for additional information, please refer to the BGCI website. Non IPEN-members have to return the Agreement on the supply of living plant material for non-commercial purposes leaving the International Plant Exchange Network , which must be signed by authorized staff. The IPEN number given with the seed material consists of four elements: 1. The first two characters are the international iso 3166-1-alpha-2-code of the country of origin ("XX" for unknown origin) 2. -
Current Plant Availability List, Including Descriptions 2021 Issue No 6: Final Autumn Stock Pelham Plants Nursery Ltd
Current plant availability list, including descriptions 2021 Issue no 6: Final autumn stock Pelham Plants Nursery Ltd Listed below are the plants currently available. Please use this list to order from us by email at [email protected] or over the phone on 07377 145970. Please use the most recent version of this list as more varieties are being added all the time. Some cultivars produced in small numbers may also sell out. We are proud of ‘home growing’ all our plants. The list will grow and change substantially as many new varieties become available week by week. It is also advisable to book to visit the nursery in person for the best range and advice. It can be difficult to keep this list up to date at our busiest times or when batches are small. We reserve the right to withdraw plants or changes prices without notice. Full explanation, delivery charges and terms and conditions are listed on our website www.pelhamplants.co.uk Plants currently Approx Price Description available pot size Acis autumnalis. AGM. 0.5L £4.50 A little 'Leucojum' now renamed Acis. Little white bonnets in autumn over grassy foliage and stems. Ideal for a focal pot. 10cm. Aconitum 'Blue Opal'. 2.0L £8.50 Opalescent violet-blue flowers in late summer. Aconitum carmichaelii 2.0L £8.50 syn. Late Vintage. Originally a seed strain, this is a valuable late 'Spätlese'. summer flowering selection with lilac-purple flowers from pale green buds. Aconitum carmichaelii 2.0L £8.50 Late summer flowering in a particularly good cobalt blue. -
Complete Chloroplast Genomes Shed Light on Phylogenetic
www.nature.com/scientificreports OPEN Complete chloroplast genomes shed light on phylogenetic relationships, divergence time, and biogeography of Allioideae (Amaryllidaceae) Ju Namgung1,4, Hoang Dang Khoa Do1,2,4, Changkyun Kim1, Hyeok Jae Choi3 & Joo‑Hwan Kim1* Allioideae includes economically important bulb crops such as garlic, onion, leeks, and some ornamental plants in Amaryllidaceae. Here, we reported the complete chloroplast genome (cpDNA) sequences of 17 species of Allioideae, fve of Amaryllidoideae, and one of Agapanthoideae. These cpDNA sequences represent 80 protein‑coding, 30 tRNA, and four rRNA genes, and range from 151,808 to 159,998 bp in length. Loss and pseudogenization of multiple genes (i.e., rps2, infA, and rpl22) appear to have occurred multiple times during the evolution of Alloideae. Additionally, eight mutation hotspots, including rps15-ycf1, rps16-trnQ-UUG, petG-trnW-CCA , psbA upstream, rpl32- trnL-UAG , ycf1, rpl22, matK, and ndhF, were identifed in the studied Allium species. Additionally, we present the frst phylogenomic analysis among the four tribes of Allioideae based on 74 cpDNA coding regions of 21 species of Allioideae, fve species of Amaryllidoideae, one species of Agapanthoideae, and fve species representing selected members of Asparagales. Our molecular phylogenomic results strongly support the monophyly of Allioideae, which is sister to Amaryllioideae. Within Allioideae, Tulbaghieae was sister to Gilliesieae‑Leucocoryneae whereas Allieae was sister to the clade of Tulbaghieae‑ Gilliesieae‑Leucocoryneae. Molecular dating analyses revealed the crown age of Allioideae in the Eocene (40.1 mya) followed by diferentiation of Allieae in the early Miocene (21.3 mya). The split of Gilliesieae from Leucocoryneae was estimated at 16.5 mya. -
Plant Life MagillS Encyclopedia of Science
MAGILLS ENCYCLOPEDIA OF SCIENCE PLANT LIFE MAGILLS ENCYCLOPEDIA OF SCIENCE PLANT LIFE Volume 4 Sustainable Forestry–Zygomycetes Indexes Editor Bryan D. Ness, Ph.D. Pacific Union College, Department of Biology Project Editor Christina J. Moose Salem Press, Inc. Pasadena, California Hackensack, New Jersey Editor in Chief: Dawn P. Dawson Managing Editor: Christina J. Moose Photograph Editor: Philip Bader Manuscript Editor: Elizabeth Ferry Slocum Production Editor: Joyce I. Buchea Assistant Editor: Andrea E. Miller Page Design and Graphics: James Hutson Research Supervisor: Jeffry Jensen Layout: William Zimmerman Acquisitions Editor: Mark Rehn Illustrator: Kimberly L. Dawson Kurnizki Copyright © 2003, by Salem Press, Inc. All rights in this book are reserved. No part of this work may be used or reproduced in any manner what- soever or transmitted in any form or by any means, electronic or mechanical, including photocopy,recording, or any information storage and retrieval system, without written permission from the copyright owner except in the case of brief quotations embodied in critical articles and reviews. For information address the publisher, Salem Press, Inc., P.O. Box 50062, Pasadena, California 91115. Some of the updated and revised essays in this work originally appeared in Magill’s Survey of Science: Life Science (1991), Magill’s Survey of Science: Life Science, Supplement (1998), Natural Resources (1998), Encyclopedia of Genetics (1999), Encyclopedia of Environmental Issues (2000), World Geography (2001), and Earth Science (2001). ∞ The paper used in these volumes conforms to the American National Standard for Permanence of Paper for Printed Library Materials, Z39.48-1992 (R1997). Library of Congress Cataloging-in-Publication Data Magill’s encyclopedia of science : plant life / edited by Bryan D.