Unraveling the structure of the Mycobacterial Envelope Mamadou Daffe, Hedia Marrakchi To cite this version: Mamadou Daffe, Hedia Marrakchi. Unraveling the structure of the Mycobacterial Envelope. Micro- biology Spectrum, American Society for Microbiology, 2019. hal-02400597 HAL Id: hal-02400597 https://hal.archives-ouvertes.fr/hal-02400597 Submitted on 9 Dec 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Unraveling the structure of the Mycobacterial Envelope Mamadou Daffé* and Hedia Marrakchi Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, Department of Tuberculosis and Infection Biology, BP 64182, 205 Route de Narbonne, 31077 Toulouse cedex 04 (France) *
[email protected] Running title: The Mycobacterial cell envelope 1 The mycobacterial cell envelope consists of a typical plasma membrane of lipid and protein, surrounded by a complex cell wall composed of carbohydrate and lipid. In pathogenic species, such as Mycobacterium tuberculosis, an outermost ‘capsule’ layer surrounds the cell wall. This wall embraces a fundamental, covalently linked ‘cell-wall skeleton’ composed of peptidoglycan, solidly attached to arabinogalactan whose penta-saccharide termini are esterified by very long-chain fatty acids (mycolic acids). These fatty acids form the inner leaflet of an outer membrane, called mycomembrane, whose outer leaflet consists of a great variety of non-covalently linked lipids and glycolipids.