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FBXO32 Promotes Microenvironment Underlying Epithelial-Mesenchymal Transition Via Ctbp1 During Tumour Metastasis and Brain Development

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FBXO32 Promotes Microenvironment Underlying Epithelial-Mesenchymal Transition Via Ctbp1 During Tumour Metastasis and Brain Development FBXO32 promotes microenvironment underlying epithelial- mesenchymal transition via CtBP1 during tumour metastasis and brain development Sahu, S. K., Tiwari, N., Pataskar, A., Zhuang, Y., Borisova, M., Diken, M., Strand, S., Beli, P., & Tiwari, V. K. (2017). FBXO32 promotes microenvironment underlying epithelial-mesenchymal transition via CtBP1 during tumour metastasis and brain development. Nature Communications, 8(1), [1523]. https://doi.org/10.1038/s41467-017-01366-x Published in: Nature Communications Document Version: Publisher's PDF, also known as Version of record Queen's University Belfast - Research Portal: Link to publication record in Queen's University Belfast Research Portal Publisher rights Copyright 2017 the authors. 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Sep. 2021 ARTICLE DOI: 10.1038/s41467-017-01366-x OPEN FBXO32 promotes microenvironment underlying epithelial-mesenchymal transition via CtBP1 during tumour metastasis and brain development Sanjeeb Kumar Sahu1, Neha Tiwari2, Abhijeet Pataskar1, Yuan Zhuang1, Marina Borisova1, Mustafa Diken3, Susanne Strand4, Petra Beli1 & Vijay K. Tiwari1 1234567890 The set of events that convert adherent epithelial cells into migratory cells are collectively known as epithelial–mesenchymal transition (EMT). EMT is involved during development, for example, in triggering neural crest migration, and in pathogenesis such as metastasis. Here we discover FBXO32, an E3 ubiquitin ligase, to be critical for hallmark gene expression and phenotypic changes underlying EMT. Interestingly, FBXO32 directly ubiquitinates CtBP1, which is required for its stability and nuclear retention. This is essential for epigenetic remodeling and transcriptional induction of CtBP1 target genes, which create a suitable microenvironment for EMT progression. FBXO32 is also amplified in metastatic cancers and its depletion in a NSG mouse xenograft model inhibits tumor growth and metastasis. In addition, FBXO32 is essential for neuronal EMT during brain development. Together, these findings establish that FBXO32 acts as an upstream regulator of EMT by governing the gene expression program underlying this process during development and disease. 1 Institute of Molecular Biology (IMB), Ackermannweg 4, 55128 Mainz, Germany. 2 Institute of Physiological Chemistry, University Medical Center, Johannes Gutenberg University, 55131 Mainz, Germany. 3 TRON - Translational Oncology at the University Medical Center of the Johannes Gutenberg University gGmbH, Freiligrathstrasse 12, 55131 Mainz, Germany. 4 Department of Internal Medicine I, University Medical Center, Johannes Gutenberg University, Obere Zahlbacher Straße 63, 55131 Mainz, Germany. Correspondence and requests for materials should be addressed to V.K.T. (email: [email protected]) NATURE COMMUNICATIONS | 8: 1523 | DOI: 10.1038/s41467-017-01366-x | www.nature.com/naturecommunications 1 ARTICLE NATURE COMMUNICATIONS | DOI: 10.1038/s41467-017-01366-x pithelial cells remain in close contact with their neighbors reorganization and loss of cell–cell junctions. This allows epi- and maintain an apical–basal axis of polarity by the thelial cells to escape from their original location by acquiring a E 2 sequential arrangement of adherent junctions, desmosomes, migratory, mesenchymal identity . Such a dramatic change in cell and tight junctions1. Following the induction of epithelial to fate is essential for key processes during embryonic development, mesenchymal transition (EMT) program, cells undergo molecular such as embryo implantation, embryonic layer formation, gas- and phenotypic remodeling that involves changes like cytoskeletal trulation and neural tube formation. In adults, this change in cell a bcd NMuMG RNA-seq EMT timecourse (NMuMG) NMuMG HMEC TGF-β Analysis of differentially expressed genes 15 ** Untreated d1 d4 d7 100 *** 46 kDa ** FBXO32 Investigation of genes induced during EMT 10 *** 42 kDa based on: literature, localization, tumor datasets * *** Lamin-B1 80 kDa 50 mRNA 5 68 kDa Expression validation in other EMT models: mRNA primary human HMEC cells, EpRAS, Neuronal EMT, e HMEC 0 (normalized to CTCF) Invasive/ non-invasive cancer cell lines (normalized to Ctcf) 0 β d1 d4 d1 d4 TGF- d7 d7 TGF-β TGF-β Untreated d1 d4 d7 Phenotype and molecular characterization 46 kDa FBXO32 upon depletion of candidate genes during EMT Untreated Untreated 42 kDa β-actin 42 kDa 32 kDa f NMuMG E-cadherin ZO-1 Paxillin Phalloidin N-cadherin Fibronectin Bright field Untrearted β siControl d4 TGF- β d4 TGF- siFbxo32-1 β d4 TGF- siFbxo32-2 g h NMuMG HMEC Untreated * Untreated β ** β d4 TGF- siControl 2000 ** 200 d4 TGF- siControl d4 TGF-β siFbxo32-1 d4 TGF-β siFBXO32-1 ** β d4 TGF-β siFbxo32-2 100 d4 TGF- siFBXO32-2 ** * 20 6 * 20 0.04 0.20 0.020 1500 ** 0.8 * ** ** ** ** ** * * ** 0.4 ** ** 15 15 0.15 * 0.6 0.03 0.015 ** 4 1000 0.2 ** * ** ** * ** ** ** ** 0.4 10 10 0.02 0.10 0.010 0.02 2 500 0.2 5 5 0.01 0.05 0.005 0.01 mRNA (normalized to CTCF) 0 0 0 0 0 0 0 0 0 mRNA (normalized to Ctcf) Fbxo32 Cdh1 Cdh2 Fn1 Snai1 Zeb2 Twist1 FN1 CDH2 SNAI1 SNAI2 ZEB1 FBXO32 CLDN3 TWIST2 2 NATURE COMMUNICATIONS | 8: 1523 | DOI: 10.1038/s41467-017-01366-x | www.nature.com/naturecommunications NATURE COMMUNICATIONS | DOI: 10.1038/s41467-017-01366-x ARTICLE fate is important for processes like tissue regeneration and wound indications of its role in cancer, the function of FBXO32 in EMT – healing1 4. However, aberrant activation of the EMT program is progression, metastasis and its contributions to the gene reg- associated with disease phenotypes such as organ fibrosis5 and ulatory circuitry underlying these processes remain completely tumor metastasis4, 6. unknown. A number of signaling pathways, such as TGF-β, FGF, EGF, Here we discover FBXO32 to be required in various contexts of HGF, Wnt/β-catenin and Notch are known to induce EMT1, 7. EMT, including in disease and development. We report that Among these, TGF-β is known to be the most potent and a FBXO32 is substantially induced during EMT and plays a critical prototypic inducer of EMT in various contexts, including devel- role in the transition towards mesenchymal identity by governing opment and cancer metastasis, whereas the others have more the required gene expression program. We show that FBXO32- context-specific functions2, 8. A large number of evidences have dependent K63-linked ubiquitination of CtBP1 is required for its established that the microenvironment plays a critical role during nuclear retention, which is essential for mediating transcriptional initiation and progression of EMT9. However, the effectors, changes via epigenetic reprogramming of EMT target genes. through which TGF-β mediates remodeling of the micro- These include various chemokines, chemokine receptors, and environment to promote EMT, remain poorly explored. It is also matrix metalloproteinases, thus promoting a suitable environ- well established that EMT relies on defined, genome-wide tran- ment promoting EMT progression. FBXO32 is also highly scriptional reprogramming10, 11. A number of transcription fac- amplified in a large panel of cancers, and its depletion severely tors are implicated in this process, including SNAIL, ZEB, and impairs the metastatic properties of cancer cells both in vitro and several basic helix-loop-helix proteins8, 10, 12. Importantly, these in vivo. Furthermore, depletion of FBXO32 during brain devel- transcription factors modulate gene expression in partnership opment impairs neuronal EMT. Together, these observations – with co-regulator proteins such as CTBPs13 15. Several studies establish FBXO32 among the most potent regulator of EMT by have reported that CtBPs form complexes with a variety of epi- governing the gene expression program that underlies this pro- genetic regulators or corepressor complexes that recruit epige- cess during both development and disease. netic regulators13, 15, 16. CtBPs are also known to undergo dynamic posttranslational modifications which influence their stability or subcellular localization14. Recent studies have sug- Results gested dispensability of the established key EMT transcription FBXO32 is essential for epithelial–mesenchymal transition.To factors in driving metastasis and further vouched for a need of – identify so far unknown regulators of EMT, we employed an exploring more potent factors driving this process17 19. established model system in which normal murine mammary Advances in proteomics have begun to highlight the role of gland epithelial cells (NMuMG) undergo TGF-β-induced EMT post-translational modifications during EMT and their enormous with high synchrony
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