List of Viruses Presenting at the Wild State a Biological Risk for Plants
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Recombination-Based Generation of the Agroinfectious Clones of Peanut
Journal of Virological Methods 237 (2016) 179–186 Contents lists available at ScienceDirect Journal of Virological Methods journal homepage: www.elsevier.com/locate/jviromet Recombination-based generation of the agroinfectious clones of Peanut stunt virus 1 1 ∗ Barbara Wrzesinska´ , Przemysław Wieczorek , Aleksandra Obrepalska-St˛ eplowska˛ Interdepartmental Laboratory of Molecular Biology, Institute of Plant Protection – National Research Institute, Władysława Wegorka˛ 20 St, 60-318, Poznan,´ Poland a b s t r a c t Article history: Full-length cDNA clones of Peanut stunt virus strain P (PSV-P) were constructed and introduced into Nico- Received 2 June 2016 tiana benthamiana plants via Agrobacterium tumefaciens. The cDNA fragments corresponding to three Received in revised form 5 September 2016 PSV genomic RNAs and satellite RNA were cloned into pGreen binary vector between Cauliflower mosaic Accepted 15 September 2016 virus (CaMV) 35S promoter and nopaline synthase (NOS) terminator employing seamless recombina- Available online 19 September 2016 tional cloning system. The plasmids were delivered into A. tumefaciens, followed by infiltration of hosts plants. The typical symptoms on systemic leaves of infected plants similar to those of wild-type PSV- Keywords: P were observed. The presence of the virus was confirmed by means of RT-PCR and Western blotting. Peanut stunt virus Re-inoculation to N. benthamiana, Phaseolus vulgaris, and Pisum sativum resulted in analogous results. Viral infectious clones cDNA Generation of infectious clones of PSV-P enables studies on virus-host interaction as well as revealing Agrobacterium tumefaciens viral genes functions. Seamless recombination © 2016 Elsevier B.V. All rights reserved. Isothermal recombination 1. Introduction stunting, vein clearing as well as the infection might be latent (Obrepalska-St˛ eplowska˛ et al., 2008a). -
Abacca Mosaic Virus
Annex Decree of Ministry of Agriculture Number : 51/Permentan/KR.010/9/2015 date : 23 September 2015 Plant Quarantine Pest List A. Plant Quarantine Pest List (KATEGORY A1) I. SERANGGA (INSECTS) NAMA ILMIAH/ SINONIM/ KLASIFIKASI/ NAMA MEDIA DAERAH SEBAR/ UMUM/ GOLONGA INANG/ No PEMBAWA/ GEOGRAPHICAL SCIENTIFIC NAME/ N/ GROUP HOST PATHWAY DISTRIBUTION SYNONIM/ TAXON/ COMMON NAME 1. Acraea acerata Hew.; II Convolvulus arvensis, Ipomoea leaf, stem Africa: Angola, Benin, Lepidoptera: Nymphalidae; aquatica, Ipomoea triloba, Botswana, Burundi, sweet potato butterfly Merremiae bracteata, Cameroon, Congo, DR Congo, Merremia pacifica,Merremia Ethiopia, Ghana, Guinea, peltata, Merremia umbellata, Kenya, Ivory Coast, Liberia, Ipomoea batatas (ubi jalar, Mozambique, Namibia, Nigeria, sweet potato) Rwanda, Sierra Leone, Sudan, Tanzania, Togo. Uganda, Zambia 2. Ac rocinus longimanus II Artocarpus, Artocarpus stem, America: Barbados, Honduras, Linnaeus; Coleoptera: integra, Moraceae, branches, Guyana, Trinidad,Costa Rica, Cerambycidae; Herlequin Broussonetia kazinoki, Ficus litter Mexico, Brazil beetle, jack-tree borer elastica 3. Aetherastis circulata II Hevea brasiliensis (karet, stem, leaf, Asia: India Meyrick; Lepidoptera: rubber tree) seedling Yponomeutidae; bark feeding caterpillar 1 4. Agrilus mali Matsumura; II Malus domestica (apel, apple) buds, stem, Asia: China, Korea DPR (North Coleoptera: Buprestidae; seedling, Korea), Republic of Korea apple borer, apple rhizome (South Korea) buprestid Europe: Russia 5. Agrilus planipennis II Fraxinus americana, -
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viruses Article The Phylogeography of Potato Virus X Shows the Fingerprints of Its Human Vector Segundo Fuentes 1, Adrian J. Gibbs 2 , Mohammad Hajizadeh 3, Ana Perez 1 , Ian P. Adams 4, Cesar E. Fribourg 5, Jan Kreuze 1 , Adrian Fox 4 , Neil Boonham 6 and Roger A. C. Jones 7,* 1 Crop and System Sciences Division, International Potato Center, La Molina Lima 15023, Peru; [email protected] (S.F.); [email protected] (A.P.); [email protected] (J.K.) 2 Emeritus Faculty, Australian National University, Canberra, ACT 2600, Australia; [email protected] 3 Plant Protection Department, Faculty of Agriculture, University of Kurdistan, Sanandaj 6617715175, Iran; [email protected] 4 Fera Science Ltd., Sand Hutton York YO41 1LZ, UK; [email protected] (I.P.A.); [email protected] (A.F.) 5 Departamento de Fitopatologia, Universidad Nacional Agraria, La Molina Lima 12056, Peru; [email protected] 6 Institute for Agrifood Research Innovations, Newcastle University, Newcastle upon Tyne NE1 7RU, UK; [email protected] 7 UWA Institute of Agriculture, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia * Correspondence: [email protected] Abstract: Potato virus X (PVX) occurs worldwide and causes an important potato disease. Complete PVX genomes were obtained from 326 new isolates from Peru, which is within the potato crop0s main Citation: Fuentes, S.; Gibbs, A.J.; domestication center, 10 from historical PVX isolates from the Andes (Bolivia, Peru) or Europe (UK), Hajizadeh, M.; Perez, A.; Adams, I.P.; and three from Africa (Burundi). Concatenated open reading frames (ORFs) from these genomes Fribourg, C.E.; Kreuze, J.; Fox, A.; plus 49 published genomic sequences were analyzed. -
Peanut Stunt Virus Infecting Perennial Peanuts in Florida and Georgia1 Carlye Baker2, Ann Blount3, and Ken Quesenberry4
Plant Pathology Circular No. 395 Fla. Dept. of Agric. & Consumer Serv. ____________________________________________________________________________________July/August 1999 Division of Plant Industry Peanut Stunt Virus Infecting Perennial Peanuts in Florida and Georgia1 Carlye Baker2, Ann Blount3, and Ken Quesenberry4 INTRODUCTION: Peanut stunt virus (PSV) has been reported to cause disease in a number of economically important plants worldwide. In the southeastern United States, PSV is widespread in forage legumes and is considered a major constraint to productivity and stand longevity (McLaughlin et al. 1992). It is one of the principal viruses associated with clover decline in the southeast (McLaughlin and Boykin 1988). In 2002, this virus (Fig. 1) was reported in the forage legume rhizoma or perennial peanut, Arachis glabrata Benth. (Blount et al. 2002). Perennial peanut was brought into Florida from Bra- zil in 1936. In general, the perennial peanut is well adapted to the light sandy soils of the southern Gulf Coast region of the U.S. It is drought-tolerant, grows well on low-fertility soils and is relatively free from disease or insect pest problems. The rela- tively impressive forage yields of some accessions makes the perennial peanut a promising warm-sea- son perennial forage legume for the southern Gulf Coast. Due to its high-quality forage, locally grown perennial peanut hay increasingly competes for the million plus dollar hay market currently satisfied by imported alfalfa (Medicago sativa L). There are ap- proximately 25,000 acres of perennial peanut in Ala- bama, Georgia and Florida combined. About 1000 acres are planted as living mulch in citrus groves. Fig. 1. A field of ‘Florigraze’ showing the yellowing symptoms of Peanut Popular forage cultivars include ‘Arbrook’ and Stunt Virus. -
Quarantine Regulation for Importation of Plants
Quarantine Requirements for The Importation of Plants or Plant Products into The Republic of China Bureau of Animal and Plant Health Inspection and Quarantine Council of Agriculture Executive Yuan In case of any discrepancy between the Chinese text and the English translation thereof, the Chinese text shall govern. Updated November 26, 2009 更新日期：2009 年 12 月 16 日 - 1 - Quarantine Requirements for The Importation of Plants or Plant Products into The Republic of China A. Prohibited Plants or Plant Products Pursuant to Paragraph 1, Article 14, Plant Protection and Quarantine Act 1. List of prohibited plants or plant products, countries or districts of origin and the reasons for prohibition: Plants or Plant Products Countries or Districts of Origin Reasons for Prohibition 1. Entire or any part of the All countries and districts 1. Rice hoja blanca virus following living plants (Tenuivirus) (excluding seeds): 2. Rice dwarf virus (1) Brachiaria spp. (Phytoreovirus) (2) Echinochloa spp. 3. Rice stem nematode (3) Panicum spp. (Ditylenchus angustus (4) Paspalum spp. Butler) (5) Oryza spp., Leersia hexandra, Saccioleps interrupta (6) Rottboellia spp. (7) Triticum aestivum 2. Entire or any part of the Asia and Pacific Region West Indian sweet potato following living plants (1) Palau weevil (excluding seeds) (2) China (Euscepes postfasciatus (1) Calystegia spp. (3) Cook Islands Fairmaire) (2) Dioscorea japonica (4) Federated States of Micronesia (3) Ipomoea spp. (5) Fiji (4) Pharbitis spp. (6) Guam (7) Kiribati (8) New Caledonia (9) Norfolk Island -
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9* PSEUDORECOMBINANTS OF CHERRY LEAF ROLL VIRUS by Stephen Michael Haber B.Sc. (Biochem.), University of British Columbia, 1975 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE in THE FACULTY OF GRADUATE STUDIES (The Department of Plant Science) We accept this thesis as conforming to the required standard THE UNIVERSITY OF BRITISH COLUMBIA July, 1979 ©. Stephen Michael Haber, 1979 In presenting this thesis in partial fulfilment of the requirements for an advanced degree at the University of British Columbia, I agree that the Library shall make it freely available for reference and study. I further agree that permission for extensive copying of this thesis for scholarly purposes may be granted by the Head of my Department or by his representatives. It is understood that copying or publication of this thesis for financial gain shall not be allowed without my written permission. Department of Plant Science The University of British Columbia 2075 Wesbrook Place Vancouver, Canada V6T 1W5 Date Jul- 27. 1Q7Q ABSTRACT Cherry leaf roll virus, as a nepovirus with a bipartite genome, can be genetically analysed by comparing the properties of distinct 'parental' strains and the pseudorecombinant isolates generated from them. In the present work, the elderberry (E) and rhubarb (R) strains were each purified and separated into their middle (M) and bottom (B) components by sucrose gradient centrifugation followed by near- equilibrium banding in cesium chloride. RNA was extracted from the the separated components by treatment with a dissociation buffer followed by sucrose gradient centrifugation. Extracted M-RNA of E-strain and B-RNA of R-strain were mixed and inoculated to a series of test plants as were M-RNA of R-strain and B-RNA of E-strain. -
The Family Closteroviridae Revised
Virology Division News 2039 Arch Virol 147/10 (2002) VDNVirology Division News The family Closteroviridae revised G.P. Martelli (Chair)1, A. A. Agranovsky2, M. Bar-Joseph3, D. Boscia4, T. Candresse5, R. H. A. Coutts6, V. V. Dolja7, B. W. Falk8, D. Gonsalves9, W. Jelkmann10, A.V. Karasev11, A. Minafra12, S. Namba13, H. J. Vetten14, G. C. Wisler15, N. Yoshikawa16 (ICTV Study group on closteroviruses and allied viruses) 1 Dipartimento Protezione Piante, University of Bari, Italy; 2 Laboratory of Physico-Chemical Biology, Moscow State University, Moscow, Russia; 3 Volcani Agricultural Research Center, Bet Dagan, Israel; 4 Istituto Virologia Vegetale CNR, Sezione Bari, Italy; 5 Station de Pathologie Végétale, INRA,Villenave d’Ornon, France; 6 Imperial College, London, U.K.; 7 Department of Botany and Plant Pathology, Oregon State University, Corvallis, U.S.A.; 8 Department of Plant Pathology, University of California, Davis, U.S.A.; 9 Pacific Basin Agricultural Research Center, USDA, Hilo, Hawaii, U.S.A.; 10 Institut für Pflanzenschutz im Obstbau, Dossenheim, Germany; 11 Department of Microbiology and Immunology, Thomas Jefferson University, Doylestown, U.S.A.; 12 Istituto Virologia Vegetale CNR, Sezione Bari, Italy; 13 Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan; 14 Biologische Bundesanstalt, Braunschweig, Germany; 15 Deparment of Plant Pathology, University of Florida, Gainesville, U.S.A.; 16 Iwate University, Morioka, Japan Summary. Recently obtained molecular and biological information has prompted the revision of the taxonomic structure of the family Closteroviridae. In particular, mealybug- transmitted species have been separated from the genus Closterovirus and accommodated in a new genus named Ampelovirus (from ampelos, Greek for grapevine). -
Papaya Ringspot Virus (Pry): a Serious Disease of Papaya
HAWAII COOPERATIVE EXTENSION SERVICE Hawaii Institute of Tropical Agriculture and Human Resources University of Hawaii at Manoa COMMODITY FACT SHEET PA-4(A) FRUIT PAPAYA RINGSPOT VIRUS (PRY): A SERIOUS DISEASE OF PAPAYA M. S. Nishina, Extension Agent, mTAHR, Hawaii County W. T. Nishijima, Extension Specialist, Plant Pathology F. Zoo, Curator, National Clonal Germplasm Repository, Hilo C. L. Chia, Extension Specialist, Horticulture R. F. L. Mau, Extension Specialist, Entomology D. O. Evans, Researeh Associate, Horticulture INTRODUCTION column. PRY infection will reduce the size of fruits; newer fruits at the top of the column will be Papaya Ringspot Virus (PRV) causes a smaller than normal. deadly disease of papaya that severely reduces production and kills the plants. Stems PRY is found in some areas of Hawaii but not Symptoms include: in others. It is very important to suppress out • "Water-soaked" spots and streaks on breaks of PRY where it occurs and to keep it from green stems and leaf petioles (Figure 4). invading new areas. PRY has no chemical cure. Control is by InsectVectors prevention, primarily through sanitation. Sani Indicators are: tation includes controlling the aphid vectors of • Aphids feeding on the younger papaya the disease and removing and destroying all plant tissues. plants infected with the disease, including • Aphids in quantity on other crops nearby. papaya plants and alternate host plants. All papaya growers, _including home gar Alternate Host Plants deners and commercial growers, need to be on The principal alternate hosts are cucurbits, guard against PRY. Anyone observing suspected such as: PRY symptoms should call the nearest Depart • Watermelon (Citrullus vulgaris Thunb.) ment of Agriculture or Cooperative Extension • Cucumber (Cucumis sativa L.) Service office. -
Idaho State Department of Agriculture Division of Plant Industries
IDAHO STATE DEPARTMENT OF AGRICULTURE DIVISION OF PLANT INDUSTRIES 2011 SUMMARIES OF PLANT PESTS, INVASIVE SPECIES, NOXIOUS WEEDS, PLANT LAB, NURSERY AND FIELD INSPECTION PROGRAMS WITH SURVEY RESULTS INTRODUCTION - ISDA’s Division of Plant Industries derives its statutory authority from multiple sections of Idaho Code, Title 22, including the Plant Pest Act, the Noxious Weed Law, the Nursery and Florist Law, and the Invasive Species Act. These laws give the Division of Plant Industries clear directives to conduct pest surveys and manage invasive species and plant pests with the purpose of protecting Idaho’s agricultural industries, which include crops, nursery and ranching, and is valued at over $4 billion. The Division also cooperates with other agencies, such as the Idaho Department of Lands (IDL), the University of Idaho (UI), the United States Forest Service (USFS), the United States Department of Agriculture (USDA), Plant Protection and Quarantine (PPQ), county governments, Cooperative Weed Management Areas (CWMA), and industry groups to protect all of Idaho’s landscapes and environments from invasive species. Finally, the Division of Plant Industries helps accomplish the broader mission of the Department of Agriculture to serve consumers and agriculture by safeguarding the public, plants, animals and the environment through education and regulation. This report summarizes the comprehensive and cooperative programs conducted during 2011 to enforce Idaho Statutes and fulfill the broader mission of the Department. APPLE MAGGOT (AM) (Rhagoletis pomonella Walsh) - In 1990, ISDA established by Administrative Rule an AM-free regulated area (the “Apple Maggot Free Zone” or AMFZ) that contains the major apple production areas of the state. -
NBPGR Okf"Kzd Izfrosnu ANNUAL REPORT 2012-2013
ISSN NO 0971-2572 NBPGR okf"kZd izfrosnu ANNUAL REPORT 2012-2013 jk"Vªh; ikni vkuqOakf'kd Laklk/u C;wjks (Hkkjrh; Ñf"k vuqLak/ku ifj"kn) iwlk ifjlj] ubZ fnYyh&110 012 NATIONAL BUREAU OF PLANT GENETIC RESOURCES (Indian Council of Agricultural Research) Pusa Campus, New Delhi - 110 012 Citation : Anonymous (2013). Annual Report of the National Bureau of Plant Genetic Resources 2012-2013, NBPGR, Pusa Campus, New Delhi, India, 186+vi p. Compiled and Edited by : Dr. Arjun Lal, Principal Scientist Dr. (Mrs.) Kavita Gupta, Principal Scientist Dr. (Mrs.) Vandana Tyagi, Principal Scientist Dr. (Mrs.) Sangita Yadav, Senior Scientist This report includes unprocessed or semi-processed data, which would form the basis of scientific papers in due course. The material contained in the report therefore may not be made use of without the written permission of the Director, National Bureau of Plant Genetic Resources, New Delhi except for quoting it for scientific reference. Published by the Director, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi-110 012, and Printed at Alpha Printographics (India), New Delhi-110 028. Tel.: 9999039940, 9811199620 CONTENTS Preface Executive Summary 1 Introduction 9 NBPGR Headquarters, New Delhi 1. Plant Exploration and Germplasm Collection 13 2. Germplasm Evaluation 19 3. Germplasm Conservation 40 4. Plant Quarantine 45 5. Germplasm Exchange 53 6. Tissue Culture and Cryopreservation 60 7. PGR Policy Planning 65 8. Agricultural Knowledge Management 67 9. Genomic Resources 70 NBPGR Regional Stations/ Base Centers 10. Regional Station, Akola 86 11. Regional Station, Bhowali 91 12. Base Center, Cuttack 96 13. -
Analysis of the Role of Bradysia Impatiens (Diptera: Sciaridae) As a Vector Transmitting Peanut Stunt Virus on the Model Plant Nicotiana Benthamiana
cells Article Analysis of the Role of Bradysia impatiens (Diptera: Sciaridae) as a Vector Transmitting Peanut Stunt Virus on the Model Plant Nicotiana benthamiana Marta Budziszewska, Patryk Fr ˛ackowiak and Aleksandra Obr˛epalska-St˛eplowska* Department of Molecular Biology and Biotechnology, Institute of Plant Protection—National Research Institute, Władysława W˛egorka20, 60-318 Pozna´n,Poland; [email protected] (M.B.); [email protected] (P.F.) * Correspondence: [email protected] or [email protected] Abstract: Bradysia species, commonly known as fungus gnats, are ubiquitous in greenhouses, nurs- eries of horticultural plants, and commercial mushroom houses, causing significant economic losses. Moreover, the insects from the Bradysia genus have a well-documented role in plant pathogenic fungi transmission. Here, a study on the potential of Bradysia impatiens to acquire and transmit the peanut stunt virus (PSV) from plant to plant was undertaken. Four-day-old larvae of B. impatiens were exposed to PSV-P strain by feeding on virus-infected leaves of Nicotiana benthamiana and then transferred to healthy plants in laboratory conditions. Using the reverse transcription-polymerase chain reaction (RT-PCR), real-time PCR (RT-qPCR), and digital droplet PCR (RT-ddPCR), the PSV RNAs in the larva, pupa, and imago of B. impatiens were detected and quantified. The presence of PSV Citation: Budziszewska, M.; genomic RNA strands as well as viral coat protein in N. benthamiana, on which the viruliferous larvae Fr ˛ackowiak,P.; were feeding, was also confirmed at the molecular level, even though the characteristic symptoms of Obr˛epalska-St˛eplowska,A. -
Viral Diseases of Cucurbits
report on RPD No. 926 PLANT December 2012 DEPARTMENT OF CROP SCIENCES DISEASE UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN VIRAL DISEASES OF CUCURBITS Most common viral diseases of cucurbits in Illinois are cucumber mosaic (Cucumber mosaic virus), papaya ringspot (Papaya ringspot virus), squash mosaic (Squash mosaic virus), watermelon mosaic (Watermelon mosaic virus), and zucchini yellow mosaic (Zucchini yellow mosaic virus). Depends on the time of infection, viral diseases could cause up to 100% yield losses in cucurbit fields in Illinois. Statewide surveys and laboratory and greenhouse tests conducted during 2004-2006 showed that Watermelon mosaic virus (WMV) was the most prevalent virus in commercial gourd, pumpkin, and squash fields in Illinois. Squash mosaic virus (SqMV) was the second most prevalent virus in commercial gourd, pumpkin, and squash fields. SqMV was detected in more counties than any other five viruses. Cucumber mosaic virus (CMV), Papaya ringspot virus (PRSV), and Zucchini yellow mosaic virus (ZYMV) were less prevalent in commercial gourd, pumpkin, and squash fields. All of five viruses were present alone and mixed in the samples tested. Earlier in the growing seasons (July and early August), single-virus infections were detected. Mixed infections were more common from mid August until the end of the growing season in October. Dual infection of WMV and SqMV was the most prevalent mixed virus infection detected in the fields. Most viruses infecting pumpkin and squash showed similar symptoms. The most common symptoms observed in the commercial fields and in the greenhouse studies were light- and dark- green mosaic, puckering, veinbanding, veinclearing, and deformation of leaves of gourd, pumpkin, and squash.