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UNIVERSITY OF THE PHILIPPINES PHILIPPINE GENOME CENTER DNA Sequencing Core Facility DDSS Identifying appropriate sequencing platforms that SCAN CODE will efficiently meet your [ ] CCFF research goals Contact Us Funded by the Department of Science and Technology (DOST) - Philippine Council for Industry, Energy, and Emerging Technology ADDRESS Philippine Genome Center Building Research and Development (PCIEERD), DNA Sequencing Core A. Ma. Regidor Street Facility (DSCF) was integrated to the day-to-day operations of the University of the Philippines Diliman University’s Philippine Genome Center by late 2013. Both the DNA Quezon City 1101, Philippines Sequencing Core Facility and the Core Facility for Bioinformatics began offering laboratory atnd technical services pertinent to LANDLINE +63 • 2 • 8981 • 8500 local 4707 genomics and bioinformatics research. EMAIL [email protected] [email protected] WEBSITE https://pgc.up.edu.ph DNA Sequencing Core Facility ABOUT THE FACILITY LOCATION Identifying appropriate sequencing strategies to efficiently meet research goals. DSCF offers a full range of DNA sequencing ATGCTGA T G C T G UGF ACTCCAA C T C C A services from single gene sequencing of capillary sequencers to CATCGATGC A T C G A T G medium and high-throughput sequencing of Illumina, Ion Torrent GAGGAATG A G G A A T and PacBio platforms. It is a central facility that accepts GTAAGAAG T A A G A A TCGATGCT C G A T G C sequencing service requests from clients, conducts quality GGAATGCTG G A A T G C T control tests, analysis and characterization to samples, and AATGGTAA A T G G T A GAATCGG A A T C G provides consultation on the selection of technology platforms in the most cost-effective, reliable and efficient way possible. SERVICES OFFERED APPLICATIONS TECHNOLOGIES + Nucleic Acid Extraction + Whole Genome Sequencing + Ion Torrent PGM (100MB- 1GB) + Nucleic Acid Quantification + Whole Exome Sequencing + Ion Torrent Proton (10GB) + Polymerase Chain Reaction (PCR) + Transcriptome Sequencing (RNA-Seq) + MiSeq RUO (15GB) + PCR Purification + 16S Metagenome Sequencing + MiSeq FGx (15 GB) + Sanger Sequencing + Amplicon / Targeted Sequencing + NovaSeq 6000 (3000 GB) + Library Preparation + Genotyping + NextSeq 500 (120 GB) + Next Generation Sequencing + PacBio Sequel (20GB) + Equipment Use + Affymetrix GeneTitan Microarray + Trainings (available upon request) Sample Preparation Library Sequencing Initial Data Data Analysis WORKFLOWWORKFLOW Preparation Processing.

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Synergistic Cytotoxicity of Renieramycin M and Doxorubicin in MCF-7 Breast Cancer Cells
marine drugs Article Synergistic Cytotoxicity of Renieramycin M and Doxorubicin in MCF-7 Breast Cancer Cells Jortan O. Tun 1,*, Lilibeth A. Salvador-Reyes 1,2 , Michael C. Velarde 3 , Naoki Saito 4 , Khanit Suwanborirux 5 and Gisela P. Concepcion 1,2,* 1 The Marine Science Institute, University of the Philippines Diliman, Quezon City 1101, Philippines; [email protected] 2 Philippine Genome Center, University of the Philippines Diliman, Quezon City 1101, Philippines 3 Institute of Biology, University of the Philippines Diliman, Quezon City 1101, Philippines; [email protected] 4 Graduate School of Pharmaceutical Sciences, Meiji Pharmaceutical University, Tokyo 204-8588, Japan; [email protected] 5 Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Center for Bioactive Natural Products from Marine Organisms and Endophytic Fungi (BNPME), Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand; [email protected] * Correspondence: [email protected] (J.O.T.); [email protected] (G.P.C.); Tel.: +632-922-3959 (J.O.T. & G.P.C.) Received: 23 July 2019; Accepted: 7 August 2019; Published: 16 September 2019 Abstract: Renieramycin M (RM) is a KCN-stabilized tetrahydroisoquinoline purified from the blue sponge Xestospongia sp., with nanomolar IC50s against several cancer cell lines. Our goal is to evaluate its combination effects with doxorubicin (DOX) in estrogen receptor positive MCF-7 breast cancer cells. MCF-7 cells were treated simultaneously or sequentially with various combination ratios of RM and DOX for 72 h. Cell viability was determined using the MTT assay. Synergism or antagonism was determined using curve-shift analysis, combination index method and isobologram analysis.
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Event Programme
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