DOCSLIB.ORG

Impact of Nutritional Management Strategies on Semen Quality of Developing Bulls

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Impact of Nutritional Management Strategies on Semen Quality of Developing Bulls University of Tennessee, Knoxville TRACE: Tennessee Research and Creative Exchange Masters Theses Graduate School 8-2019 Impact of nutritional management strategies on semen quality of developing bulls Jarret Proctor University of Tennessee, [email protected] Follow this and additional works at: https://trace.tennessee.edu/utk_gradthes Recommended Citation Proctor, Jarret, "Impact of nutritional management strategies on semen quality of developing bulls. " Master's Thesis, University of Tennessee, 2019. https://trace.tennessee.edu/utk_gradthes/5538 This Thesis is brought to you for free and open access by the Graduate School at TRACE: Tennessee Research and Creative Exchange. It has been accepted for inclusion in Masters Theses by an authorized administrator of TRACE: Tennessee Research and Creative Exchange. For more information, please contact [email protected]. To the Graduate Council: I am submitting herewith a thesis written by Jarret Proctor entitled "Impact of nutritional management strategies on semen quality of developing bulls." I have examined the final electronic copy of this thesis for form and content and recommend that it be accepted in partial fulfillment of the equirr ements for the degree of Master of Science, with a major in Animal Science. Jason Smith, Major Professor We have read this thesis and recommend its acceptance: Justin Rhinehart, Lew Strickland Accepted for the Council: Dixie L. Thompson Vice Provost and Dean of the Graduate School (Original signatures are on file with official studentecor r ds.) Impact of nutritional management strategies on semen quality of developing bulls A Thesis Presented for the Master of Science Degree The University of Tennessee, Knoxville Jarret Allen Proctor August 2019 i ACKNOWLEDGMENTS First and foremost, I want to thank my parents for their continued support and encouragement to chase my dreams, wherever that may take me. Secondly, my siblings, along with Shelbi Perry were tremendous supporters throughout this process and I will be eternally grateful for each of them. The friendships I have made throughout my time in Tennessee are those that will last well beyond my years at this institution, and I am thankful for the connections I have developed. Without my research assistants, consisting of Connor Biehler, Kyla-Jo Farmer, Nate Long, Abigayle Pollock, John Robert Snoddy, Kelly Melvin, and Monica Creazzo, these projects would not have been possible. Additionally, I want to express my gratitude to the Department of Animal Science at the University of Tennessee for funding this opportunity to expand my educational and technical skillset. Additionally, my committee members, Dr. Justin Rhinehart and Dr. Lew Strickland were immensely valuable to my program and I will continue to value their knowledge and insight within their respective fields of study. Most importantly, I want to thank Dr. Jason Smith, my mentor, for allowing me to serve as his first graduate student. I am grateful that his guidance and passion for animal agriculture has pushed my education and development further than I ever thought possible, the knowledge and experience I have accrued under his direction will last a lifetime. Finally, I want to express my gratitude towards the Tennessee Beef Industry Council and Jonathan Perry of Deer Valley Farms for their support of these research projects. ii ABSTRACT Nutritional management and semen collection method are thought to influence semen quality parameters evaluated during a breeding soundness examination. Therefore, the objective of this experiment was to evaluate the effects of two feeding strategies on growth performance and semen quality of developing bulls. A secondary objective was to quantify the effects of two semen collection methods on semen quality. Angus bulls (n = 48; 332 ± 47 d of age) were stratified into one of sixteen drylot pens before random assignment to one of two iso-caloric diets: a total mixed ration (TMR; n = 8 pens) or a component-based ration (COMP; n = 8 pens). Bulls were assigned to either electroejaculation (EE; n = 24) or trans-rectal massage (RM; n = 24) and each collection method occurred twice throughout an 84-d feeding period. Rumen pH was continuously monitored via rumen boluses and dry matter intake (DMI) was measured daily. Backfat thickness and hoof conformation were measured on d 0 and 84, with body weight measured and semen collected at 21-d intervals. Semen was assessed for progressive motility, total sperm per ejaculate, and sperm cell morphology. Bulls fed the COMP ration had a greater DMI (P < 0.01) and ADG (P < 0.01), but similar feed efficiency (P = 0.19) when compared to TMR-fed bulls. Bulls fed COMP had a lower mean rumen pH (P < 0.01). Additionally, COMP-fed bulls had greater backfat thickness (P < 0.01) but similar hoof conformation scores (P ≥ 0.55) when compared to TMR-fed bulls on d 84. Bulls fed the COMP had greater semen volume (P < 0.05) but feeding management did not affect progressive motility (P = 0.42), total sperm per ejaculate (P = 0.86), or percent morphologically-normal sperm (P = 0.59). However, bulls collected via RM had greater total sperm per ejaculate (P < 0.05), and semen volume (P < 0.01), but lower percent morphologically-normal sperm (P < 0.05) and progressive motility (P < 0.01). Neither diet nor semen collection method affected BSE passage rate (P ≥ 0.34), suggesting minimal influence on BSE outcomes in developing bulls. iii TABLE OF CONTENTS CHAPTER 1: Literature review ....................................................................................... 1 Introduction ..................................................................................................................... 2 Etiology of SARA ........................................................................................................... 3 Overview ..................................................................................................................... 3 Energy and starch digestion ......................................................................................... 3 Organic acid by-products of ruminal fermentation ..................................................... 6 Rumen pH as an indicator of acidosis ....................................................................... 10 Rumen temperature as an indicator of acidosis ......................................................... 13 Rumen osmolality and damage to ruminal papillae .................................................. 14 Identification and implications of SARA ..................................................................... 15 Parakeratosis, rumenitis, and liver abscesses ............................................................ 16 Laminitis/lameness .................................................................................................... 18 Fecal composition ...................................................................................................... 21 Variation in DMI ....................................................................................................... 22 Treatment and prevention of SARA ............................................................................. 25 Ration composition and physically-effective fiber .................................................. 26 Transition diets, ration blending, and bunk and feeding management .................... 28 Ionophores................................................................................................................ 30 iv Buffers...................................................................................................................... 32 Nutritional influences on bull reproduction .................................................................. 33 Pre-weaning nutritional management ...................................................................... 34 Post-weaning development ...................................................................................... 39 SARA in developing bulls ....................................................................................... 43 References ..................................................................................................................... 46 CHAPTER 2: Impact of feeding management strategies on growth performance, rumen conditions, and semen quality of developing bulls .................................................. 66 Abstract ......................................................................................................................... 67 Introduction ................................................................................................................... 68 Materials and methods .................................................................................................. 69 Experimental design and feeding management ....................................................... 70 Feed intake, sampling, re-formulation, and analysis ............................................... 72 Growth performance and hoof conformation .......................................................... 73 Rumen pH and temperature ..................................................................................... 74 Scrotal circumference, semen collection, and semen quality .................................. 74 Statistical analyses ................................................................................................... 77 Results and discussion .................................................................................................
Load more

Recommended publications
  • Novel Low-Voltage Electro-Ejaculation Approach for Sperm Collection from Zoo Captive Lanyu Miniature Pigs (Sus Barbatus Sumatranus)
    animals Article Novel Low-Voltage Electro-Ejaculation Approach for Sperm Collection from Zoo Captive Lanyu Miniature Pigs (Sus barbatus sumatranus) 1,2, 3, 3,4, 3 5 Yu-Hsin Chen y, Jane-Fang Yu y, Yu-Jia Chang y, Shih-Chien Chin , Lih-Chiann Wang , Hsiu-Lien Lin 1 and Pei-Shiue Tsai 4,5,6,* 1 Livestock Research Institute—Xinhua Branch, Council of Agriculture, Executive Yuan, Tainan 71246, Taiwan; [email protected] (Y.-H.C.); [email protected] (H.-L.L.) 2 Department of Animal Science, National Chiayi University, Chiayi 60004, Taiwan 3 Conservation and Research Center, Taipei Zoo, 30 Xinguang Road, Section 2, Wenshan, Taipei 11656, Taiwan; [email protected] (J.-F.Y.); [email protected] (Y.-J.C.); [email protected] (S.-C.C.) 4 Graduate Institute of Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan 5 Department of Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan; [email protected] 6 Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, 10617 Taipei, Taiwan * Correspondence: [email protected]; Tel.: +886-2-33661290; Fax: +886-2-23661475 These authors contributed equally to this work. y Received: 11 September 2020; Accepted: 3 October 2020; Published: 7 October 2020 Simple Summary: Preservation of genetic materials from zoo animals is important to improve genetic diversity and to prolong the existence of endangered species for future retrieval. Methods to acquire sperm samples from the animals can be achieved by hand penile massage or by rectal stimulation with electric probe. Traditional electrical stimulation applies relatively high voltage to achieve sufficient stimulation; however, this method causes stress and discomfort to the animal.
    [Show full text]
  • Instructions for Semen Collection
    For Semen analysis or cryopreservation Semen Collection Instruction and Requisition Form 1. Semen Analysis appointments are at the USF Health South Tampa Campus (2 Tampa General Circle, 4th floor, Tampa, FL 33606) from 9:50 to 11:10 am on Tuesday, Wednesday and Friday. 2. To book an appointment call 813-259-0692 for scheduling (press 2). Please let the scheduler know if you will be dropping off or collecting your specimen at our facility when you book the appointment. MUST PROVIDE PHOTO ID AT TIME OF VISIT. 3. You need to refrain from ejaculation by either intercourse or masturbation for 2-5 days prior to the collection. Shorter or longer periods of abstinence may cause abnormal results. 4. If you collect the semen at home, time from collection to dropping off should be no more than 60 minutes. 5. If you do not have insurance coverage, the charge for the semen analysis is $100. Payment is due at the time of dropping off. Patients with insurance will have this test submitted by our Financial Specialist to their insurance carrier. 6. We recommend booking at least two weeks in advance. 7. Results will be obtained with your next Physician appointment Please use following instructions to collect your semen sample: 1. Complete the information below. 2. Wash hands with alcohol wipes. 3. Clean your penis with the Alcohol-Prep (provided) and dry well. 4. Produce sample by manual masturbation. 5. Collect all semen in the specimen container provided. 6. Place lid back on the container, make sure lid is secure. 7.
    [Show full text]
  • Surrogacy & Egg Donor Journeys Amidst Travel Restrictions
    Sam Everingham Director Growing Families Surrogacy & Egg Donor Journeys amidst Travel Restrictions January 2021 Are clinics even open for IVF & surrogacy? We need an egg donor, but cant travel to provide sperm, Can we ship sperm across borders? I have Donor Embryos Stuck Overseas Can I bring them home? Can I travel for embryo transfer? January 2021 I have embryos in my home state, but need a surrogate Can I ship them for surrogacy? I don’t have embryos ready, Can I make them at home and ship overseas? What are the costs and timelines? January 2021 Are OS destinations open for IVF/Surrogacy? Surrogacy Open Ease of shipping Destinations IVF Destinations US √ More complex for sperm South Africa √ Canada √ More complex for sperm Spain √ Colombia √ straightforward Georgia X Greece √ More complex Ukraine √ Significant paperwork Mexico √ Expensive Cyprus √ More complex Albania √ unknown January 2021 Can overseas donors still travel to Australia? No Can I ship eggs? Yes, but thaw rates are not so good Better to ship embryos or sperm January 2021 I have Donor Embryos Stuck Overseas January 2021 Our embryos are in the US/ Greece/ Spain/ South Africa…. Can we ship the embryos back to Australia? Australian IVF clinics wont accept embryos unless • Donor is identity release/known (No flexibility here) • Donor was altruistic • Donor has had counselling offered If in Victoria, your application needs to go to VARTA board meeting In other states at the discretion of each IVF clinic January 2021 Our embryos are in the US/ Greece/ Spain/ South Africa…. Can I travel overseas or interstate to have an embryo transfer? Yes Growing Families has assisted >15 intended mums to travel in the last 3 months to US, Spain, Greece or South Africa January 2021 Our embryos are in the US/ Greece/ Spain/ South Africa….
    [Show full text]
  • Patient Instructions Semen Collection Information Sheet Please Read and Follow the Instructions Carefully
    Patient Instructions Semen Collection Information Sheet Please read and follow the instructions carefully. Sperm are very time and temperature sensitive, so strict adherence to the instructions below is mandatory, or there is a risk of the sample being rejected. Post Vasectomy: It is recommended that the first post vasectomy semen sample be collected after at least 12 ejaculations and 8-16 weeks post vasectomy. Fertility Testing: Specimen must be received within one (1) hour of collection by the drop off location. Note: Not all LifeLabs locations perform this testing. Please call our Customer Care Centre at 1-877-849-3637 to schedule a time, date and a drop-off location for your specimen. Specimens received without appointment will be rejected. Date and Time of Appointment and Location: ____________________________________ Collection Instruction for Seminal Fluid 1. Do Not have sex or masturbate for 48 hours before collecting the sample. 2. Abstain from sex or masturbation for no more than seven (7) days. 3. Using only the container provided, produce specimen by masturbation, collecting the entire ejaculate directly into the container. Note: Specimen containers, other than that which is provided, are unacceptable for collection and will be rejected. Do not use a sheath/condom or lubricants for collection, as they are harmful to sperm. Masturbation is the only acceptable method of collection. If a portion of the specimen is lost, the sample should be discarded and collection attempted at a later date using a new kit. 4. Seal the container immediately after specimen collection; make sure that the lid is on properly and tightly.
    [Show full text]
  • Guide to Male Infertility
    P (678) 344 8900 F (678) 666 5201 [email protected] Guide to Male Infertility MALE INFERTILITY: MANY CAUSES, MANY TREATMENTS Approximately 15% of couples have difficulty achieving a pregnancy during their reproductive years. For nearly half of these couples, a male factor is involved — either as the sole cause, or as a contributing cause of infertility. Male infertility stems from a variety of sources, and today can be corrected with a number of treatments. Significant progress has been made in the past decade in the diagnosis and treatment of male infertility. Many men who just a few years ago had little chance of fathering a child, can now have their problems corrected and have a biological child of their own. Should I Be Evaluated? In the past, couples experiencing fertility problems were advised to postpone a diagnostic workup until they’d been trying to conceive for at least a year. Generally, when they did begin infertility testing, only the female was evaluated. Today we know that this kind of delay can waste valuable time and lessen a couple’s chances for conceiving. Couples are now encouraged to be evaluated as soon as they suspect a fertility problem, especially if the wife is over 35 years old, and men should begin their diagnostic workup at the same time as their female partner. The man’s workup should be performed by a male fertility specialist — usually a urologist who focuses on male fertility, and has some postgraduate training in this area. Evaluating the Male Patient: First Steps Taking a Medical History A male fertility evaluation starts with a thorough medical history.
    [Show full text]
  • ARTIFICIAL INSEMINATION(For Veterinarians) 3) Introduction and Semen Quality(For Final Year Students) by Prof.Dr
    1) Successful application of AI in cattle By Prof.Dr. Sayed Hattab Prof. of Theriogenology Alex Universityِ 2) ARTIFICIAL INSEMINATION(for veterinarians) 3) Introduction and Semen Quality(for final year students) By Prof.Dr. AHMED MAMDOUH OSMAN Department of Theriogenology Faculty of Veterinary Medicine Assiut University 4) ARTIFICIAL INSEMINATION By Prof.Dr.Afyfi El-Monofy Faculty of Veterinary Medicine Cairo University 1 copyright@ E V E T C -O59-I0 Prof.Dr.M.El Naggar (PM) ARTIFICIAL INSEMINATION (BY Prof.Dr.Sayed hattab) Definition Artificial insemination is a process by which sperm are collected from male, processed, stored and artificially introduced into the female reproductive tract for the purpose of conception. History of A.I 1322, Arab Chieftain stole semen from stallion owned by an enemy 1677, Leeuwenhook used microscope to see sperm 1780, Spallanzani reported a successful AI in dog. 1900, Ivanov of Russia Pioneered AI research in livestock, he was the first to successfully inseminate cattle 1936, Denmark was the first to establish an AI cooperative association 1938 USA first AI cooperative association at New Jersey. 1960, 1970, become popular in cattle. 1990, expansion o horse and swine AI. Objectives of A.I Genetic improvement Diseases control mechanism Possible to increase fertility Decrease breeding expense Advantages of A.I Genetic improvement wide spread use and availability of genetically superior sires. One bull can breed 500,000 cows in a life time 2 copyright@ E V E T C -O59-I0 Prof.Dr.M.El Naggar (PM)
    [Show full text]
  • Artificial Insemination in Marsupials
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by ResearchOnline at James Cook University Available online at www.sciencedirect.com Theriogenology 71 (2009) 176–189 www.theriojournal.com Artificial insemination in marsupials John C. Rodger a,*, Damien B.B.P. Paris b, Natasha A. Czarny a, Merrilee S. Harris a, Frank C. Molinia c, David A. Taggart d, Camryn D. Allen e, Stephen D. Johnston e a School of Environmental and Life Sciences, The University of Newcastle, NSW 2308, Australia b Department of Equine Sciences, Faculty of Veterinary Medicine, Universiteit Utrecht, 3584 CM Utrecht, The Netherlands c Landcare Research, Private Bag 92170, Auckland 1142, New Zealand d Royal Zoological Society of South Australia, Frome Rd, Adelaide, SA 5000, Australia e School of Animal Studies, The University of Queensland, Gatton 4343, Australia Abstract Assisted breeding technology (ART), including artificial insemination (AI), has the potential to advance the conservation and welfare of marsupials. Many of the challenges facing AI and ART for marsupials are shared with other wild species. However, the marsupial mode of reproduction and development also poses unique challenges and opportunities. For the vast majority of marsupials, there is a dearth of knowledge regarding basic reproductive biology to guide an AI strategy. For threatened or endangered species, only the most basic reproductive information is available in most cases, if at all. Artificial insemination has been used to produce viable young in two marsupial species, the koala and tammar wallaby. However, in these species the timing of ovulation can be predicted with considerably more confidence than in any other marsupial.
    [Show full text]
  • Collection, Analysis and Cryopreservation of Semen from Malayan Gaur (Bos Gaurus Hubbacki): a Preliminary Study
    Open Veterinary Journal, (2012), Vol. 2: 109-114 ISSN: 2226-4485 (Print) ISSN: 2218-6050 (Online) Original Article _____________________________________________________________________________________ Submitted: 05/07/2012 Accepted: 07/10/2012 Published: 31/10/2012 Collection, analysis and cryopreservation of semen from Malayan gaur (Bos gaurus hubbacki): A preliminary study M.I. Iswadi1,*, Z.F. Ann1, M.M. Hafiz2, M.D. Hafiz2, F.J. Fahrul2, H. Hajarian3, H. Wahid3, I. Zawawi4, M.S. Khairiah5 and O.A. Mazni6 1Veterinary Research Institute (VRI), Department of Veterinary Services, 59, Jalan Sultan Azlan Shah, 31400 Ipoh, Perak, Malaysia 2Agro-Biotechnology Institute, Malaysia (ABI), Ministry of Science, Technology and Innovation, P.O Box 341, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia 3Veterinary Hospital, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia 4National Institute of Veterinary Biodiversity, Department of Veterinary Services, Bukit Dinding, 27000 Jerantut, Pahang, Malaysia 5Department Of Wildlife and National Parks, Pahang, Bangunan Asia Life, Jalan Teluk Sisek, 25000 Kuantan, Pahang, Malaysia 6Malaysian Agricultural Research and Development Institute (MARDI), 43400 Serdang, Selangor, Malaysia _____________________________________________________________________________________________ Abstract The Malayan gaur (Bos gaurus hubbacki) or Seladang is classified as vulnerable by the International Union for Conservation of Nature and Natural Resources (IUCN). The Malayan
    [Show full text]
  • Introduction: Being a Sperm Donor
    INTRODUCTION BEING A SPERM DONOR perm donation is probably one of reproductive biomedicine’s Smost long-standing endeavors. On offi cial record since at least the middle of the eighteenth century (Ombelet and Robays 2010), the practice of collecting semen in a container through masturbation for purposes of artifi cial insemination has been around for about 250 years. Together with donor insemination, it has ever since func- tioned as a low-tech solution for childlessness and infertility requir- ing no laboratory or clinical equipment. Having a man willing and able to masturbate into a container, a woman wanting to undergo insemination, and a person prepared to carry out the insemination procedure is all that is required, and while today’s sperm donation and donor insemination involve semen collection and testing at sperm banks and insemination procedures at either a clinic or at home with specialized insemination kits, sperm donation and donor insemination can also be carried out using everyday objects such as cups and turkey basters. The relatively ready availability of donor semen and sperm do- nation’s low-tech status are certainly part of what made it into a viable success. At the same time, however, sperm donation is also reproductive biomedicine’s stepchild, so to say, since the use of do- nor semen in reproductive biomedicine goes against at least three long-standing Euro-American social taboos: masturbation, infi del- ity, and multilineal kinship. It is sperm donation’s reliance on men masturbating in order to produce semen, its invocation of infi del relations between sperm donor and donor semen recipient, and its disturbance of bilineal kinship that stir moral concerns about the use of donor semen in reproductive biomedicine.
    [Show full text]
  • Welcome to ACRM!
    1 ACRM Welcome to ACRM! Thanks for making an appointment! Your doctor has requested we assist them in evaluating your sperm count by doing a semen analysis. Each of our offices perform semen analysis. We will provide the test result to your doctor within a few days of your visit. The following pages outline what you’ll need to know about doing this test. Beyond basic medical care and the full range of Assisted Reproductive Technologies (IVF, Donor Egg, Frozen Egg), we also offer psychological support, in- house acupuncture, and nutrition services should you need them. To make things easier and more successful for you, we have 4 locations, 7 physicians, 14 nurses, 3 embryologists and whole team of support staff available to you. Our website provides more information about us (www.acrm.com). We look forward to your visit! Lisa Hasty, MD Andre Denis, MPH, MD Jim Toner, MD, PhD Robin Fogle, MD Sue Ellen Carpenter, MD David Keenan, MD Kathryn C. Calhoun, MD Steven A. Voelkel, PhD, HCLD 2 ACRM Office Hours, Locations, & Emergency Contact Johns Creek 6470 East Johns Crossing Suite 200 Johns Creek, GA 30097 OPEN: Mon to Fri, 8 am to 4 pm Marietta 711 Canton Road Suite 410 Atlanta Center for Marietta, GA 30060 Reproductive Medicine OPEN: Mon to Fri, 8 am to 4 pm Perimeter (main) 5909 Peachtree Dunwoody Road Suite 600 Atlanta, GA 30328 OPEN: Mon to Fri, 8 am to 4 pm Sat, Sun, holidays, 8 am to noon Buckhead 1800 Howell Mill Road Suite 675 Atlanta, GA 30318 OPEN: Mon to Fri, 8 am to 4 pm Note: We are open on weekends and most holidays until noon (only for certain appointment types) Phone: (770) 928-2276 (for all offices) Emergencies / After Hours: (770) 928-2276 3 ACRM For your visit… Prior to your first visit with us, please: ☐Review the information about your semen analysis ☐Fill out the Patient Information Sheet, and fax to (678) 303-0482 or email to: [email protected].
    [Show full text]
  • The Process of Sperm Cryopreservation, Thawing and Washing Techniques
    Chapter 14 The Process of Sperm Cryopreservation, Thawing and Washing Techniques Sajal Gupta, Rakesh Sharma, and Ashok Agarwal Introduction Reproduction is a key need which is central to human experience and drives quality of life. There is a growing list of conditions wherein there is a detrimental impact on fertility as a result of the disease itself or the therapeutic interventions directed towards the treatment of the disease conditions such as cancer or other extreme medical conditions. Sperm cryopreservation is used for these men, as they may desire to have children in the future, but current circumstances prevent the certainty that they will be able to conceive through traditional methods. This can be because of dangerous military deployment overseas, cancer or other toxic treatments which cause infertility, and many other situations. Sperm cryopreservation procedure involves the collection of the sample, freezing and long-term change storing to storage of the sample in extremely low temperature under liquid nitrogen at −196° C for possible use in the future to help achieve parenthood. All men who undergo treatments detrimental to repro- ductive function or face the challenge of their fertility being impacted by chemo- therapy or radiation therapy should exercise the option of semen collection for cryopreservation [1]. Cryopreservation of human semen is an important proce- dure for preservation of gametes in patients undergoing gonadotoxic treatment. Even some fertile couples can experience difficulties in conceiving due to geo- graphical or professional challenges and a general deterioration of male repro- ductive health over time is also an important indication for sperm banking. In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) have radi- cally advanced the treatment of male-factor infertility.
    [Show full text]
  • SEMEN COLLECTION PROTOCOL Instructions for a Patient Who Is to Collect a Semen Specimen for the Study of Male Fertility
    Department of Pathology and Laboratory Medicine SEMEN COLLECTION PROTOCOL Instructions for a patient who is to collect a semen specimen for the study of male fertility. 1. The patient must pick up the specimen cup and protocol from the Outpatient Laboratory, Ground Floor at the North Entrance of the hospital or the doctor’s office before collecting the specimen. 2. The patient should have no sexual relations or ejaculations for at least 2-7 days before collecting the specimen. 3. The optimal method for specimen collection is by masturbation directly into the container provided. Do not use a condom unless you are certain that it contains no spermicides or sperm inhibiting chemicals. You may be assured that such a single masturbation is not harmful in any way. It is very important that all of the semen from the first ejaculation be collected; loss of a portion will give a misleading analysis. 4. Immediately after collection, screw the lid firmly on the container. Write your name, social security number and the date and time the specimen was collected on the container. 5. Immediately bring the specimen to the Outpatient Laboratory for billing and paperwork processing; testing must begin within one hour after collection. Failure to examine the specimen within one hour of collection may result in a misleading analysis. 6. During transportation, do not agitate the container in any way. Avoid direct sunlight, extreme heat or cold. The sample can be kept warm by keeping the container close to the body. IMPORTANT Specimens will be accepted daily from 7:00 am to 3:00 pm.
    [Show full text]