processes Review Ultrafiltration Method for Plasma Protein Binding Studies and Its Limitations Camelia-Maria Toma 1,2, Silvia Imre 1,2,3,*, Camil-Eugen Vari 4 , Daniela-Lucia Muntean 3 and Amelia Tero-Vescan 5 1 Center of Advanced Medical and Pharmaceutical Research, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 38 Gheorghe Marinescu Street, RO-540142 Targu Mures, Romania;
[email protected] 2 Doctoral School of Medicine and Pharmacy, I.O.S.U.D., George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 38 Gheorghe Marinescu Street, RO-540142 Targu Mures, Romania 3 Department of Analytical Chemistry and Drug Analysis, Faculty of Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 38 Gheorghe Marinescu Street, RO-540142 Targu Mures, Romania;
[email protected] 4 Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 38 Gheorghe Marinescu Street, RO-540142 Targu Mures, Romania;
[email protected] 5 Department of Chemistry and Medical Biochemistry, Faculty of Medicine in English, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 38 Gheorghe Marinescu Street, RO-540142 Targu Mures, Romania;
[email protected] * Correspondence:
[email protected] Abstract: Plasma protein binding plays a critical role in drug therapy, being a key part in the charac- terization of any compound. Among other methods, this process is largely studied by ultrafiltration based on its advantages. However, the method also has some limitations that could negatively influence the experimental results.