Bone Induction of Human Tooth and Bone Crushed by Newly Developed Automatic Mill
Journal of the Ceramic Society of Japan 118 [6] 434-437 2010 Paper Bone induction of human tooth and bone crushed by newly developed automatic mill Masaru MURATA,³ Toshiyuki AKAZAWA,* Masahiko TAKAHATA,** Manabu ITO,** Junichi TAZAKI,*** Jun HINO,*** Katsuo NAKAMURA,* Norimasa IWASAKI,** Takanori SHIBATA*** and Makoto ARISUE Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Tobetsu, Hokkaido 061–0293 *Materials Technology, Hokkaido Industrial Research Institute, Kita 19 Nishi 11, Kita-ku, Sapporo, Hokkaido 060–0819 **Orthopaedic Surgery, Hokkaido University Graduate School of Medicine, Kita 14 Nishi 7, Kita-ku, Sapporo, Hokkaido 060–8586 ***Reconstructive Surgery for Oral and Maxillofacial Region, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Tobetsu, Hokkaido 061-0293 A novel automaticmill of teeth and bone has been developed for bone engineering. A human frozen-tooth and/or a human frozen-bone block were put into the Zirconium oxide (ZrO2) ceramics vessel of the machine, and crushed for 1 minwith 20 saline- 3 ice blocks (1 © 1 © 1cm /block) at 12000 rpm of ZrO2 blade. The crushed granules were demineralized completely in2% HNO3 solution for 20 min, and rinsed incoldsaline. We named each biomaterial after the acid treatment and washing, demineralized dentin matrices (DDM), demineralized bone matrices (DBM). Five wisdom teeth (total wet volume: 10.0 g) were crushed, decalcified, and lyophilized. The distribution offreeze-dried DDM granules was fine granules (0.51.0 mm: 0.27 g), moderate (1.02.0 mm: 0.46 g), and large (2.05.0 mm: 0.64 g). The fine granules of human DDM or DBM were implanted into the subcutaneous tissue of 4 week-old nude mice, and theirtissue-inductive properties were estimated at 4 weeks after implantation histologically.
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