DOCSLIB.ORG

An Integrative Taxonomic Assessment of Procamallanus (Spirocamallanus

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
An Integrative Taxonomic Assessment of Procamallanus (Spirocamallanus Parasitology Research (2019) 118:2819–2829 https://doi.org/10.1007/s00436-019-06429-0 FISH PARASITOLOGY - ORIGINAL PAPER An integrative taxonomic assessment of Procamallanus (Spirocamallanus) huacraensis (Nematoda: Camallanidae), infecting the freshwater catfish Trichomycterus spegazzinii (Siluriformes: Trichomycteridae) in Argentina Lorena G. Ailán-Choke1 & Dora A. Davies1 & Luiz E.R. Tavares2 & Felipe B. Pereira2 Received: 2 May 2019 /Accepted: 15 August 2019 /Published online: 28 August 2019 # Springer-Verlag GmbH Germany, part of Springer Nature 2019 Abstract Procamallanus (Spirocamallanus) huacraensis infecting the catfish Trichomycterus spegazzinii from Escoipe River, Salta province (Argentina), is redescribed and genetically characterised for the first time, based on three genetic markers (nuclear 18S and 28S rRNA; cytochrome c oxidase subunit I [cox1] mtDNA). The phylogeny of Camallanidae was also discussed. Morphological evaluation of P.(S.) huacraensis using light and scanning electron microscopy revealed the previously undescribed features: location of deirids, accurate morphology of larvae (L1) and ovijector in females, as well as phasmids in males. Differences were found comparing the newly collected material and the type specimens, probably because the original description lacked detailing. Unfortunately, type specimens of P.(S.) huacraensis were no available for loan. The results of morphological and genetic analyses supported the validity of P.(S.) huacraensis. Inconsistencies regarding the taxonomic identification of species of Camallanidae in GenBank database were noted. Based on the current genetic database of Camallanidae, phylogenetic reconstructions using the 18S rRNA sequences were most consistent, due to the inclusion of higher number of taxa. Procamallanus (S.) huacraensis appeared as sister group of P.(S.) rarus, also isolated from a catfish in a neighbouring region. The order and habitat of hosts were also similar within some well-supported parasite lineages, but without common geographic origin. However, it is still premature to make definitive affirmations regarding the role of such features in the phylogenetic patterns of Camallanidae, given the scarcity of genetic data. The phylogenetic reconstructions also confirmed the artificiality of the morphology-based systematics of the family. Keywords Nematode parasite . Genetic characterisation . Phylogeny . Neotropical region Introduction Section Editor: Guillermo Salgado-Maldonado Camallanidae Railliet & Henry, 1915 is a monophyletic fam- Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00436-019-06429-0) contains supplementary ily of nematodes that infect marine and freshwater fishes, material, which is available to authorized users. exhibiting broad global distribution (Anderson et al. 2009; Černotíková et al. 2011). Despite its species richness, propor- * Felipe B. Pereira tionally few taxa within the Camallanidae has been genetically [email protected] characterised (Wu et al. 2008, 2009;Kuzminetal.2011; Gaither et al. 2013), and their phylogenetic relationships are 1 Instituto para el Estudio de la Biodiversidad de Invertebrados, Facultad de Ciencias Naturales, Universidad Nacional de Salta, Av. poorly understood (Sardella et al. 2017). Bolivia 5150, (4400), Salta, Argentina The morphological taxonomy of camallanids is problematic, 2 Programa de Pós-Graduação em Biologia Animal, Instituto de especially regarding species of Procamallanus Baylis, 1923 Biociências, Universidade Federal de Mato Grosso do Sul, Av. Costa (Sardella et al. 2017). This genus has been subdivided into sev- e Silva s/n°,, Campo Grande, MS 79070-900, Brazil eral subgenera based on the structure of the buccal capsule, a fact 2820 Parasitol Res (2019) 118:2819–2829 that generates debate (see Moravec and Sey 1988; Moravec and The systematic classification of nematodes follows the pro- Thatcher 1997; Rigby and Adamson 1997; Anderson et al. 2009; posal of Moravec and Thatcher (1997). Voucher specimens Moravec and Van As 2015). The current genetic database does were deposited in the Parasitological Collection of the not support the resolution of such systematic problems Museo Argentino de Ciencias Naturales ‘Bernardino (Černotíková et al. 2011;Sardellaetal.2017). Rivadavia’, Buenos Aires, Argentina (MACN-Pa). Procamallanus (Spirocamallanus) Olsen, 1952, is the most specious subgenera of Procamallanus; however, several spe- Genetic procedures cies remain poorly described and only a few representatives were genetically characterised, also resulting in taxonomic Genomic DNA was isolated using DNeasy Blood & Tissue problems (Moravec and Justine 2017;Sardellaetal.2017). Kit (QIAGEN, Hilden, Germany), following the manufac- In this sense, improvement of the morphological and genetic turer’s instructions. Three genetic regions were amplified: knowledge pertaining to these parasites is necessary. the 5′ end of the 18S nuclear rRNA, the D2 and D3 domains During parasitological examinations in Trichomycterus of the nuclear 28S rRNA and the cox1 of the mtDNA. All spegazzinii (Berg) (Siluriformes: Trichomycteridae) from PCR reactions, cycling conditions and primers are detailed River Escoipe, province of Salta, Argentina, some specimens in Online Resource 1. PCR products were purified through of nematodes were recovered from the anterior intestine of the an enzymatic treatment with ExoProStar™ (GE Helathcare) fish. Detailed observation revealed that the specimens were and sent for sequencing at ACTGene (Ludwig Biotec, Rio similar to P.(S.) huacraensis Ramallo 2008 (a species with Grande do Sul, Brazil) with the same primers used in PCR poorly detailed morphological description). Here, we reactions. redescribed P.(S.) huacraensis, using light and scanning elec- Contiguous sequences were assembled in Geneious tron microscopy and provided its first genetic characterisation (Geneious ver. 9.1.5 created by Biomatters, available from based on nuclear and mitochondrial genetic markers. http://www.geneious.com/) and deposited in the GenBank Additionally, phylogenetic reconstructions were used to eval- (see taxonomic summary for accession numbers). uate the phylogenetic position of P.(S.) huacraensis within Preliminary BLAST search on GenBank database (https:// Camallanidae and its relationships with other taxa. Some as- www.ncbi.nlm.nih.gov/genbank/) was performed to confirm pects of the phylogeny of Camallanidae were also discussed. the genetic proximity between the present sequences and those from representatives of Camallanidae. Materials and methods Phylogenetic analyses of molecular data Collection and examination of nematodes The phylogenetic reconstructions were based on two different datasets, one containing sequences of the 18S rRNA and one During February 2017 to March 2019, 111 specimens of containing those of cox1 mtDNA. Due to the small number of T. spegazzinii (standard length 33.00–162.79 mm) were sequences of the 28S rRNA, phylogenies based on this genetic caught using fishing rods in Escoipe River (25°06′49″S; region were provided as Online Resource 2 and were not 65°36′0.4″W), municipality of Chicoana, Salta province, discussed here. Sequences were chosen according to the fol- Argentina. Fish were kept alive in small water tanks and lowing criteria: genetic regions congruent with those obtained brought to the laboratory prior to helminthological examina- in the present study and minimum length of 744 bp and 355 bp tions. Host nomenclature and classification were updated ac- for the 18S and the cox1, respectively (for details see Table 1). cording to Froese and Pauly (2019). Nematodes were found Sequences from the same isolate, clones or with 100% of alive, washed in saline, fixed in hot 4% formaldehyde solution genetic similarity were mostly not considered. The outgroup and preserved in 70% ethanol. For morphological observa- was chosen according to previous phylogenies of tions, nematodes were cleared in glycerine. Two males had Camallanidae (see Černotíková et al. 2011). Sequences were the mid body excised and fixed in molecular-grade 96–99% aligned using M-Cofee (Notredame et al. 2000), then evaluat- ethanol for genetic studies; anterior and posterior parts were ed by the transitive consistency score, to verify the reliability fixed from morphological identification. of aligned positions and, based on score values, ambiguous Drawings were made using a drawing tube attached to a aligned positions were trimmed (Chang et al. 2014). Datasets microscope Olympus BX51. Measurements are given in were subjected to maximum likelihood (ML) and Bayesian micrometres, unless otherwise stated. Specimens used for inference (BI) analyses, using PHYML and MrBayes, respec- SEM (2 males and 2 females) were dehydrated through a tively (Huelsenbeck and Ronquist 2001; Guindon and graded ethanol series, dried by evaporation with Gascuel 2003). The model of evolution (nucleotide substitu- hexamethyldisilazane, coated with gold and observed in a tion) and its fixed parameters for each dataset were chosen and JEOL JSM 6460-LV, at an accelerating voltage of 15 kV. estimated under the Akaike information criterion with Parasitol Res (2019) 118:2819 Table 1 Species whose sequences were obtained from GenBank and used in phylogenetic reconstructions, associated with their hosts (habitat), geographic origin, accession number and genetic regions. The 18S and 28S refer to the rRNA gene and the cox1 to the cytochrome c oxidase subunit I mtDNA. Superscript
Load more

Recommended publications
  • Universidade Federal De Pernambuco Centro De Biociências Programa De Pós-Graduação Em Biologia Animal
    UNIVERSIDADE FEDERAL DE PERNAMBUCO CENTRO DE BIOCIÊNCIAS PROGRAMA DE PÓS-GRADUAÇÃO EM BIOLOGIA ANIMAL CAMILA NASCIMENTO DE OLIVEIRA HISTÓRIA NATURAL E ASPECTOS ECOLÓGICOS DE COLEODACTYLUS MERIDIONALIS (BOULENGER, 1888) EM UM FRAGMENTO DE MATA ATLÂNTICA, PERNAMBUCO, NORDESTE, BRASIL RECIFE 2016 CAMILA NASCIMENTO DE OLIVEIRA HISTÓRIA NATURAL E ASPECTOS ECOLÓGICOS DE COLEODACTYLUS MERIDIONALIS (BOULENGER, 1888) EM UM FRAGMENTO DE MATA ATLÂNTICA, PERNAMBUCO, NORDESTE, BRASIL Dissertação apresentada ao Programa de Pós-Graduação em Biologia Animal, Área de Concentração Zoologia, da Universidade Federal de Pernambuco, como requisito parcial para obtenção do título de mestre em Biologia Animal. Orientadora: Drª. Míriam Camargo Guarnieri Co-orientador: Dr. Samuel Cardozo Ribeiro RECIFE 2016 Catalogação na fonte Elaine Barroso CRB 1728 Oliveira, Camila Nascimento de História natural e aspectos ecológicos de Coleodactylus meridionalis (Boulenger, 1888) em um fragmento de Mata Atlântica, Pernambuco, Nordeste, Brasil / Camila Nascimento de Oliveira- Recife: O Autor, 2016. 88 folhas: il., fig., tab. Orientadora: Miriam Camargo Guarnieri Coorientador: Samuel Cardozo Ribeiro Dissertação (mestrado) – Universidade Federal de Pernambuco. Centro de Biociências. Biologia Animal, 2016. Inclui referências e apêndice 1. Lagartos 2. Florestas tropicais 3. Ecologia I. Guarnieri, Miriam Camargo (orientadora) II. Ribeiro, Samuel Cardozo (coorientador) III. Título 597.95 CDD (22.ed.) UFPE/CB-2017-275 CAMILA NASCIMENTO DE OLIVEIRA HISTÓRIA NATURAL E ASPECTOS ECOLÓGICOS DE COLEODACTYLUS MERIDIONALIS (BOULENGER, 1888) EM UM FRAGMENTO DE MATA ATLÂNTICA, PERNAMBUCO, NORDESTE, BRASIL Dissertação apresentada ao Programa de Pós-Graduação em Biologia Animal, Área de Concentração Zoologia, da Universidade Federal de Pernambuco, como requisito parcial para obtenção do título de mestre em Biologia Animal. Aprovada em: 29/07/2016 COMISSÃO EXAMINADORA ___________________________ ____________________________ Dr.
    [Show full text]
  • Some Aspects of the Taxonomy and Biology of Adult Spirurine Nematodes Parasitic in Fishes: a Review
    FOLIA PARASITOLOGICA 54: 239–257, 2007 REVIEW ARTICLE Some aspects of the taxonomy and biology of adult spirurine nematodes parasitic in fishes: a review František Moravec Institute of Parasitology, Biology Centre, Academy of Sciences of the Czech Republic, Branišovská 31, 370 05 České Budějovice, Czech Republic Key words: Nematoda, Spirurina, Cystidicolidae, Rhabdochonidae, parasites, fish, taxonomy, biology Abstract. About 300 species belonging to four superfamilies (Gnathostomatoidea, Habronematoidea, Physalopteroidea and Thelazioidea) of the nematode suborder Spirurina are known as the adult parasites of freshwater, brackish-water and marine fishes. They are placed in four families, of which the Gnathostomatidae, including Echinocephalus with a few species and the monotypic Metaleptus, are parasites of elasmobranchs, whereas Ancyracanthus contains one species in teleosts; the Physalopteri- dae is represented in fish by four genera, Bulbocephalus, Heliconema, Paraleptus and Proleptus, each with several species in both elasmobranchs and teleosts. The majority of fish spirurines belongs to the Rhabdochonidae, which includes 10 genera (Beaninema, Fellicola, Hepatinema, Heptochona, Johnstonmawsonia, Megachona, Pancreatonema, Prosungulonema, Rhabdo- chona and Vasorhabdochona) of species parasitizing mainly teleosts, rarely elasmobranchs, and the Cystidicolidae with about 23 genera (Ascarophis, Caballeronema, Capillospirura, Comephoronema, Crenatobronema, Cristitectus, Ctenascarophis, Cyclo- zone, Cystidicola, Cystidicoloides, Johnstonmawsonoides,
    [Show full text]
  • Luth Wfu 0248D 10922.Pdf
    SCALE-DEPENDENT VARIATION IN MOLECULAR AND ECOLOGICAL PATTERNS OF INFECTION FOR ENDOHELMINTHS FROM CENTRARCHID FISHES BY KYLE E. LUTH A Dissertation Submitted to the Graduate Faculty of WAKE FOREST UNIVERSITY GRADAUTE SCHOOL OF ARTS AND SCIENCES in Partial Fulfillment of the Requirements for the Degree of DOCTOR OF PHILOSOPHY Biology May 2016 Winston-Salem, North Carolina Approved By: Gerald W. Esch, Ph.D., Advisor Michael V. K. Sukhdeo, Ph.D., Chair T. Michael Anderson, Ph.D. Herman E. Eure, Ph.D. Erik C. Johnson, Ph.D. Clifford W. Zeyl, Ph.D. ACKNOWLEDGEMENTS First and foremost, I would like to thank my PI, Dr. Gerald Esch, for all of the insight, all of the discussions, all of the critiques (not criticisms) of my works, and for the rides to campus when the North Carolina weather decided to drop rain on my stubborn head. The numerous lively debates, exchanges of ideas, voicing of opinions (whether solicited or not), and unerring support, even in the face of my somewhat atypical balance of service work and dissertation work, will not soon be forgotten. I would also like to acknowledge and thank the former Master, and now Doctor, Michael Zimmermann; friend, lab mate, and collecting trip shotgun rider extraordinaire. Although his need of SPF 100 sunscreen often put our collecting trips over budget, I could not have asked for a more enjoyable, easy-going, and hard-working person to spend nearly 2 months and 25,000 miles of fishing filled days and raccoon, gnat, and entrail-filled nights. You are a welcome camping guest any time, especially if you do as good of a job attracting scorpions and ants to yourself (and away from me) as you did on our trips.
    [Show full text]
  • Parasitic Nematodes of Pool Frog (Pelophylax Lessonae) in the Volga Basin
    Journal MVZ Cordoba 2019; 24(3):7314-7321. https://doi.org/10.21897/rmvz.1501 Research article Parasitic nematodes of Pool Frog (Pelophylax lessonae) in the Volga Basin Igor V. Chikhlyaev1 ; Alexander B. Ruchin2* ; Alexander I. Fayzulin1 1Institute of Ecology of the Volga River Basin, Russian Academy of Sciences, Togliatti, Russia 2Mordovia State Nature Reserve and National Park «Smolny», Saransk, Russia. *Correspondence: [email protected] Received: Febrary 2019; Accepted: July 2019; Published: August 2019. ABSTRACT Objetive. Present a modern review of the nematodes fauna of the pool frog Pelophylax lessonae (Camerano, 1882) from Volga basin populations on the basis of our own research and literature sources analysis. Materials and methods. Present work consolidates data from different helminthological works over the past 80 years, supported by our own research results. During the period from 1936 to 2016 different authors examined 1460 specimens of pool frog, using the method of full helminthological autopsy, from 13 regions of the Volga basin. Results. In total 9 nematodes species were recorded. Nematode Icosiella neglecta found for the first time in the studied host from the territory of Russia and Volga basin. Three species appeared to be more widespread: Oswaldocruzia filiformis, Cosmocerca ornata and Icosiella neglecta. For each helminth species the following information included: systematic position, areas of detection, localization, biology, list of definitive hosts, the level of host-specificity. Conclusions. Nematodes of pool frog, excluding I. neglecta, belong to the group of soil-transmitted helminthes (geohelminth) and parasitize in adult stages. Some species (O. filiformis, C. ornata, I. neglecta) are widespread in the host range.
    [Show full text]
  • DNA Barcode) De Espécies De Bagres (Ordem Siluriformes) De Valor Comercial Da Amazônia Brasileira
    UNIVERSIDADE DO ESTADO DO AMAZONAS ESCOLA DE CIÊNCIAS DA SAÚDE PROGRAMA DE PÓS-GRADUAÇÃO EM BIOTECNOLOGIA E RECURSOS NATURAIS DA AMAZÔNIA ELIZANGELA TAVARES BATISTA Código de barras de DNA (DNA Barcode) de espécies de bagres (Ordem Siluriformes) de valor comercial da Amazônia brasileira MANAUS 2017 ELIZANGELA TAVARES BATISTA Código de barras de DNA (DNA Barcode) de espécies de bagres (Ordem Siluriformes) de valor comercial da Amazônia Brasileira Dissertação apresentada ao Programa de Pós- Graduação em Biotecnologia e Recursos Naturais da Amazônia da Universidade do Estado do Amazonas (UEA), como parte dos requisitos para obtenção do título de mestre em Biotecnologia e Recursos Naturais Orientador: Prof Dra. Jacqueline da Silva Batista MANAUS 2017 ELIZANGELA TAVARES BATISTA Código de barras de DNA (DNA Barcode) de espécies de bagres (Ordem Siluriformes) de valor comercial da Amazônia Brasileira Dissertação apresentada ao Programa de Pós- Graduação em Biotecnologia e Recursos Naturais da Amazônia da Universidade do Estado do Amazonas (UEA), como parte dos requisitos para obtenção do título de mestre em Biotecnologia e Recursos Naturais Data da aprovação ___/____/____ Banca Examinadora: _________________________ _________________________ _________________________ MANAUS 2017 Dedicatória. À minha família, especialmente ao meu filho Miguel. Nada é tão nosso como os nossos sonhos. Friedrich Nietzsche AGRADECIMENTOS A Deus, por me abençoar e permitir que tudo isso fosse possível. À Dra. Jacqueline da Silva Batista pela orientação, ensinamentos e pela paciência nesses dois anos. À CAPES pelo auxílio financeiro. Ao Programa de Pós-Graduação em Biotecnologia e Recursos Naturais da Amazônia MBT/UEA. À Coordenação do Curso de Pós-Graduação em Biotecnologia e Recursos Naturais da Amazônia.
    [Show full text]
  • Phylogenetic Relationships of the South American Doradoidea (Ostariophysi: Siluriformes)
    Neotropical Ichthyology, 12(3): 451-564, 2014 Copyright © 2014 Sociedade Brasileira de Ictiologia DOI: 10.1590/1982-0224-20120027 Phylogenetic relationships of the South American Doradoidea (Ostariophysi: Siluriformes) José L. O. Birindelli A phylogenetic analysis based on 311 morphological characters is presented for most species of the Doradidae, all genera of the Auchenipteridae, and representatives of 16 other catfish families. The hypothesis that was derived from the six most parsimonious trees support the monophyly of the South American Doradoidea (Doradidae plus Auchenipteridae), as well as the monophyly of the clade Doradoidea plus the African Mochokidae. In addition, the clade with Sisoroidea plus Aspredinidae was considered sister to Doradoidea plus Mochokidae. Within the Auchenipteridae, the results support the monophyly of the Centromochlinae and Auchenipterinae. The latter is composed of Tocantinsia, and four monophyletic units, two small with Asterophysus and Liosomadoras, and Pseudotatia and Pseudauchenipterus, respectively, and two large ones with the remaining genera. Within the Doradidae, parsimony analysis recovered Wertheimeria as sister to Kalyptodoras, composing a clade sister to all remaining doradids, which include Franciscodoras and two monophyletic groups: Astrodoradinae (plus Acanthodoras and Agamyxis) and Doradinae (new arrangement). Wertheimerinae, new subfamily, is described for Kalyptodoras and Wertheimeria. Doradinae is corroborated as monophyletic and composed of four groups, one including Centrochir and Platydoras, the other with the large-size species of doradids (except Oxydoras), another with Orinocodoras, Rhinodoras, and Rhynchodoras, and another with Oxydoras plus all the fimbriate-barbel doradids. Based on the results, the species of Opsodoras are included in Hemidoras; and Tenellus, new genus, is described to include Nemadoras trimaculatus, N.
    [Show full text]
  • Hlístice Vybraných Druhů Studenokrevných Obratlovců Západní Afriky Diplomová Práce
    MASARYKOVA UNIVERZITA Přírodovědecká fakulta Ústav botaniky a zoologie Hlístice vybraných druhů studenokrevných obratlovců západní Afriky Diplomová práce Brno 2008 autor: Bc. Šárka Mašová Vedoucí DP: RNDr. Božena Koubková, Ph.D. PROHLÁŠENÍ Souhlasím s uložením této diplomové práce v knihovně Ústavu botaniky a zoologie PřF MU v Brně, případně v jiné knihovně MU, s jejím veřejným půjčováním a využitím pro vědecké, vzdělávací nebo jiné veřejně prospěšné účely, a to za předpokladu, že převzaté informace budou řádně citovány a nebudou využívány komerčně. Brno, 19. května 2008 …………………………….. PODĚKOVÁNÍ Ráda bych poděkovala vedoucí mé diplomové práce RNDr. Boženě Koubkové, Ph.D. za její odborné vedení a praktické rady. Velice děkuji svému konzultantovi prof. Ing. Vlastimilu Barušovi, DrSc. za cenné rady a pomoc při zpracování problematiky taxonomie nematod, prof. RNDr. Františkovi Tenorovi, DrSc. za pomoc s determinací tasemnic, dále Mgr. Ivetě Matějusové, Ph.D. za molekulární analýzy, Mgr. Ivetě Hodové za zasvěcení do SEM a Mgr. Radimovi Sonnekovi za zasvěcení do CLSM a v neposlední řadě Doc. RNDr. Petru Koubkovi, CSc. za poskytnutí studijního materiálu. Také děkuji všem, kteří mi jakýmkoliv způsobem pomohli při zpracování této diplomové práce a svým nejbližším za podporu. Práce byla finančně podporována Grantovou agenturou AV ČR, grant číslo IAA6093404 a výzkumným záměrem Masarykovy university v Brně číslo MSM 0021622416. ABSTRAKT Za účelem studia parazitických hlístic ryb Senegalu bylo v letech 2004 – 2006 vyšetřeno 330 jedinců náležejících ke 49 sladkovodním druhům ryb. Většina vyšetřených ryb pocházela z národního parku Nikolo Koba ve východním Senegalu. Celkem byly determinovány 3 rody parazitických hlístic ve 24 druzích ryb (prevalence 71%) z 9 čeledí. Nalezená fauna hlístic se skládala většinou ze zástupců čeledi Camallanidae.
    [Show full text]
  • A Revision of the Genus Oxysomatium Railliet & Henry, ]916
    Bull. Mus. nain. Hist. nat., Paris, 4e sér., 2, 1980, section A, n° 3 : 707-718. A revision of the genus Oxysomatium Railliet & Henry, ]916 (Nematoda, Cosmocercidae) by Michael R. BAKER * Résumé. — Révision du genre Oxysomatium Railliet (f Henry, 1916 [Nematoda, Cosmocer- cidae). — Le genre Neoxysomatium Ballesteros-Mârquez, 1945, est synonyme A'Oxysomatium Bailliet & Henry, 1916 ; ces deux genres ont la même espèce-type. Oxysomatium comprend trois espèces : O. brevicaudatum (Zeder, 1800) [= O. contortum (Linstow, 1906) Baylis, 1927 ; = O. longe- spiculum Railliet & Henry, 1916] ; O. caucasicum (Sharpilo, 1974) n. comb. [= Neoxysomatium caucasicum et O. dollfusi n. sp. O. dollfusi se distingue des autres espèces par les dimensions et la morphologie des spicules et par la répartition géographique. Toutes les autres espèces classées précédemment dans ce genre doivent être assignées à des genres différents. La femelle ^Oxysoma- tium waltoni Skrjabin, Schikhobalova & Mozgovoi, 1951, est inconnue et cette espèce est laissée incertae sedis. Oxysomatium dogieli Skrjabin, 1916, est species dubia. O. tibetanum Baylis, 1927, est transféré dans le genre Cosmocercoides et O. baylisi Walton, 1933, est transféré dans le genre Railliet' nema. Oxysomatium georgianum Reiber, Byrd & Parker, 1940, est un synonyme de Cosmocercoides dukae (Holl). Abstract. — The genus Neoxysomatium Ballesteros-Mârquez, 1945, is shown to be synonymous with Oxysomatium Railliet & Henry, since the type species are the same. Oxysomatium contains only the three following species : O. brevicaudatum (Zeder, 1800) [= O. contortum (Linstow, 1906) Baylis, 1927 ; = O. longespiculum Railliet & Henry, 1916] ; O. caucasicum (Sharpilo, 1974) n. comb. [= Neoxysomatium caucasicum] ; O. dollfusi n. sp. O. dollfusi differs from the other two species in the size and morphology of the spicules and in geographical distribution.
    [Show full text]
  • Nematoda; Cosmocercidae
    An Acad Bras Cienc (2020) 92(2): e20180499 DOI 10.1590/0001-3765202020180499 Anais da Academia Brasileira de Ciências | Annals of the Brazilian Academy of Sciences Printed ISSN 0001-3765 I Online ISSN 1678-2690 www.scielo.br/aabc | www.fb.com/aabcjournal BIOLOGICAL SCIENCES A new species of Cosmocercoides Running title: NEW SPECIES OF Cosmocercoides IN Leptodactylus latrans (Nematoda; Cosmocercidae) and other helminths in Leptodactylus latrans (Anura; Academy Section: Health Sciences Leptodactylidae) from Argentina e20180499 REGINA DRAGHI, FABIANA B. DRAGO & LÍA I. LUNASCHI Abstract: Cosmocercoides latrans n. sp. (Cosmocercidae) from the small intestine of 92 (2) Leptodactylus latrans (Anura: Leptodactylidae) from Northeastern Province of Buenos 92(2) Aires, Argentina is described. The new species can be distinguished from their congeners by a combination of the characters, among which stands out the number of rosette papillae, the lack of gubernaculum and the presence of lateral alae in both sexes. There are over 20 species in the genus Cosmocercoides, and Cosmocercoides latrans n. sp. represents the third species from the Neotropical realm and the second for Argentina. Additionally, seven previously known taxa are reported; Pseudoacanthocephalus cf. lutzi, Catadiscus uruguayensis, Rauschiella palmipedis, Aplectana hylambatis, Cosmocerca parva, Schrankiana sp. and Rhabdias elegans; providing literature records and information on distribution and host-parasite relationships. Key words: helminths, Leptodactylus latrans, Cosmocercoides latrans n. sp., anura, Argentina. INTRODUCTION Venezuela, the savanna areas of Guyana, Brazil, northeastern Bolivia, eastern Paraguay, Previous reports of endoparasites in Argentina, and Uruguay (Heyer et al. 2010). Being Leptodactylus latrans (Steffen, 1815) have been an opportunistic feeder, its diet is generalist summarized in checklists from South America and determined by the availability of prey in the (Campião et al.
    [Show full text]
  • Guide to the Parasites of Fishes of Canada Part V: Nematoda
    Wilfrid Laurier University Scholars Commons @ Laurier Biology Faculty Publications Biology 2016 ZOOTAXA: Guide to the Parasites of Fishes of Canada Part V: Nematoda Hisao P. Arai Pacific Biological Station John W. Smith Wilfrid Laurier University Follow this and additional works at: https://scholars.wlu.ca/biol_faculty Part of the Biology Commons, and the Marine Biology Commons Recommended Citation Arai, Hisao P., and John W. Smith. Zootaxa: Guide to the Parasites of Fishes of Canada Part V: Nematoda. Magnolia Press, 2016. This Book is brought to you for free and open access by the Biology at Scholars Commons @ Laurier. It has been accepted for inclusion in Biology Faculty Publications by an authorized administrator of Scholars Commons @ Laurier. For more information, please contact [email protected]. Zootaxa 4185 (1): 001–274 ISSN 1175-5326 (print edition) http://www.mapress.com/j/zt/ Monograph ZOOTAXA Copyright © 2016 Magnolia Press ISSN 1175-5334 (online edition) http://doi.org/10.11646/zootaxa.4185.1.1 http://zoobank.org/urn:lsid:zoobank.org:pub:0D054EDD-9CDC-4D16-A8B2-F1EBBDAD6E09 ZOOTAXA 4185 Guide to the Parasites of Fishes of Canada Part V: Nematoda HISAO P. ARAI3, 5 & JOHN W. SMITH4 3Pacific Biological Station, Nanaimo, British Columbia V9R 5K6 4Department of Biology, Wilfrid Laurier University, Waterloo, Ontario N2L 3C5. E-mail: [email protected] 5Deceased Magnolia Press Auckland, New Zealand Accepted by K. DAVIES (Initially edited by M.D.B. BURT & D.F. McALPINE): 5 Apr. 2016; published: 8 Nov. 2016 Licensed under a Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0 HISAO P. ARAI & JOHN W.
    [Show full text]
  • Ahead of Print Online Version Phylogenetic Relationships of Some
    Ahead of print online version FOLIA PARASITOLOGICA 58[2]: 135–148, 2011 © Institute of Parasitology, Biology Centre ASCR ISSN 0015-5683 (print), ISSN 1803-6465 (online) http://www.paru.cas.cz/folia/ Phylogenetic relationships of some spirurine nematodes (Nematoda: Chromadorea: Rhabditida: Spirurina) parasitic in fishes inferred from SSU rRNA gene sequences Eva Černotíková1,2, Aleš Horák1 and František Moravec1 1 Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, Branišovská 31, 370 05 České Budějovice, Czech Republic; 2 Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic Abstract: Small subunit rRNA sequences were obtained from 38 representatives mainly of the nematode orders Spirurida (Camalla- nidae, Cystidicolidae, Daniconematidae, Philometridae, Physalopteridae, Rhabdochonidae, Skrjabillanidae) and, in part, Ascaridida (Anisakidae, Cucullanidae, Quimperiidae). The examined nematodes are predominantly parasites of fishes. Their analyses provided well-supported trees allowing the study of phylogenetic relationships among some spirurine nematodes. The present results support the placement of Cucullanidae at the base of the suborder Spirurina and, based on the position of the genus Philonema (subfamily Philoneminae) forming a sister group to Skrjabillanidae (thus Philoneminae should be elevated to Philonemidae), the paraphyly of the Philometridae. Comparison of a large number of sequences of representatives of the latter family supports the paraphyly of the genera Philometra, Philometroides and Dentiphilometra. The validity of the newly included genera Afrophilometra and Carangi- nema is not supported. These results indicate geographical isolation has not been the cause of speciation in this parasite group and no coevolution with fish hosts is apparent. On the contrary, the group of South-American species ofAlinema , Nilonema and Rumai is placed in an independent branch, thus markedly separated from other family members.
    [Show full text]
  • Redescription and Molecular Characterisation Of
    Russian Journal of Nematology, 2019, 27 (1), 57 – 66 Redescription and molecular characterisation of Comephoronema werestschagini Layman, 1933 (Nematoda: Cystidicolidae) from the endemic Baikal fish Cottocomephorus grewingkii (Dybowski, 1874) (Scorpaeniformes: Cottocomephoridae) with some comments on cystidicolid phylogeny Sergey G. Sokolov, Ekaterina L. Voropaeva and Svetlana V. Malysheva Centre of Parasitology, A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninskii Prospect, 33, Moscow, 119071, Russia e-mail: [email protected] Accepted for publication 12 September 2019 Summary. The nematode Comephoronema werestschagini (Chromadorea: Cystidocolidae) is redescribed from the Baikal yellowfin Cottocomephorus grewingkii (Dybowski, 1874). Important morphological features, such as the presence of four submedian cephalic papillae, four well developed bilobed sublabia, two large lateral pseudolabia, pairs of rounded deirids, as well as the position of phasmids are reported for the first time in this species. The SSU rDNA-based phylogeny of the cystidicolids is defined according to the sequences obtained for C. werestschagini, C. oshmarini and Capillospirura ovotrichuria. The polyphyly of the Cystidocolidae is herein confirmed. All three studied species appear as members of Cystidicolidae s. str. clade; however, C. werestschagini and C. oshmarini are not phylogenetically related. Key words: 18S rDNA, Capillospirura ovotrichuria, Cystidicolidae, nematodes, phylogeny, SEM. The genus Comephoronema Layman, 1933 scorpaeniform fishes, but a number of authors have (Nematoda: Chromadorea: Cystidicolidae) includes noticed this species in some other Baikal fishes, in five species of nematodes, parasitic in freshwater particular Brachymystax lenok (Pallas, 1773) fish of Eurasia, as well as some marine fish of the (Salmonidae), Lota lota (Linnaeus, 1758) (Lotidae) and Atlantic and Antarctic Oceans (Moravec et al., Thymallus arcticus (Pallas, 1776) (Salmonidae) s.
    [Show full text]