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Unnatural Amino Acid Incorporation to Rewrite The
Unnatural Amino Acid Incorporation to Rewrite the Genetic Code and RNA-peptide Interactions Thesis by Xin Qi In partial fulfillment of the requirements for the degree of Doctor of Philosophy California Institute of Technology Pasadena, California 2005 (Defended May 19, 2005) ii © 2005 Xin Qi All Rights Reserved iii Acknowledgments I like to sincerely thank my advisor, Dr. Richard W. Roberts, for his great mentorship over the course of my graduate career here at Caltech. This thesis would have not been possible without his tremendous scientific vision and a great deal of guidance. I am also in debt to Dr. Peter Dervan, Dr. Robert Grubbs, and Dr. Stephen Mayo, who serve on my thesis committee. They have provided very valuable help along the way that kept me on track in the pursuit of my degree. I have been so fortunate to have been working with a number of fantastic colleagues in Roberts’ group. Particularly, I want express my gratitude toward Shelley R. Starck, with whom I had a productive collaboration on one of my projects. Members of Roberts group, including Shuwei Li, Terry Takahashi, Ryan J. Austin, Bill Ja, Steve Millward, Christine Ueda, Adam Frankel, and Anders Olson, all have given me a great deal of help on a number of occasions in my research, and they are truly fun people to work with. I also like to thank our secretary, Margot Hoyt, for her support during my time at Caltech. I have also collaborated with people from other laboratories, e.g., Dr. Cory Hu from Prof. Varshavsky’s group, and it has been a positive experience to have a nature paper together. -
The Analects of Confucius
The analecTs of confucius An Online Teaching Translation 2015 (Version 2.21) R. Eno © 2003, 2012, 2015 Robert Eno This online translation is made freely available for use in not for profit educational settings and for personal use. For other purposes, apart from fair use, copyright is not waived. Open access to this translation is provided, without charge, at http://hdl.handle.net/2022/23420 Also available as open access translations of the Four Books Mencius: An Online Teaching Translation http://hdl.handle.net/2022/23421 Mencius: Translation, Notes, and Commentary http://hdl.handle.net/2022/23423 The Great Learning and The Doctrine of the Mean: An Online Teaching Translation http://hdl.handle.net/2022/23422 The Great Learning and The Doctrine of the Mean: Translation, Notes, and Commentary http://hdl.handle.net/2022/23424 CONTENTS INTRODUCTION i MAPS x BOOK I 1 BOOK II 5 BOOK III 9 BOOK IV 14 BOOK V 18 BOOK VI 24 BOOK VII 30 BOOK VIII 36 BOOK IX 40 BOOK X 46 BOOK XI 52 BOOK XII 59 BOOK XIII 66 BOOK XIV 73 BOOK XV 82 BOOK XVI 89 BOOK XVII 94 BOOK XVIII 100 BOOK XIX 104 BOOK XX 109 Appendix 1: Major Disciples 112 Appendix 2: Glossary 116 Appendix 3: Analysis of Book VIII 122 Appendix 4: Manuscript Evidence 131 About the title page The title page illustration reproduces a leaf from a medieval hand copy of the Analects, dated 890 CE, recovered from an archaeological dig at Dunhuang, in the Western desert regions of China. The manuscript has been determined to be a school boy’s hand copy, complete with errors, and it reproduces not only the text (which appears in large characters), but also an early commentary (small, double-column characters). -
(And Misreading) the Draft Constitution in China, 1954
Textual Anxiety Reading (and Misreading) the Draft Constitution in China, 1954 ✣ Neil J. Diamant and Feng Xiaocai In 1927, Mao Zedong famously wrote that a revolution is “not the same as inviting people to dinner” and is instead “an act of violence whereby one class overthrows the authority of another.” From the establishment of the People’s Republic of China (PRC) in 1949 until Mao’s death in 1976, his revolutionary vision became woven into the fabric of everyday life, but few years were as violent as the early 1950s.1 Rushing to consolidate power after finally defeating the Nationalist Party (Kuomintang, or KMT) in a decades- long power struggle, the Chinese Communist Party (CCP) threatened the lives and livelihood of millions. During the Land Reform Campaign (1948– 1953), landowners, “local tyrants,” and wealthier villagers were targeted for repression. In the Campaign to Suppress Counterrevolutionaries in 1951, the CCP attacked former KMT activists, secret society and gang members, and various “enemy agents.”2 That same year, university faculty and secondary school teachers were forced into “thought reform” meetings, and businessmen were harshly investigated during the “Five Antis” Campaign in 1952.3 1. See Mao’s “Report of an Investigation into the Peasant Movement in Hunan,” in Stuart Schram, ed., The Political Thought of Mao Tse-tung (New York: Praeger, 1969), pp. 252–253. Although the Cultural Revolution (1966–1976) was extremely violent, the death toll, estimated at roughly 1.5 million, paled in comparison to that of the early 1950s. The nearest competitor is 1958–1959, during the Great Leap Forward. -
The Later Han Empire (25-220CE) & Its Northwestern Frontier
University of Pennsylvania ScholarlyCommons Publicly Accessible Penn Dissertations 2012 Dynamics of Disintegration: The Later Han Empire (25-220CE) & Its Northwestern Frontier Wai Kit Wicky Tse University of Pennsylvania, [email protected] Follow this and additional works at: https://repository.upenn.edu/edissertations Part of the Asian History Commons, Asian Studies Commons, and the Military History Commons Recommended Citation Tse, Wai Kit Wicky, "Dynamics of Disintegration: The Later Han Empire (25-220CE) & Its Northwestern Frontier" (2012). Publicly Accessible Penn Dissertations. 589. https://repository.upenn.edu/edissertations/589 This paper is posted at ScholarlyCommons. https://repository.upenn.edu/edissertations/589 For more information, please contact [email protected]. Dynamics of Disintegration: The Later Han Empire (25-220CE) & Its Northwestern Frontier Abstract As a frontier region of the Qin-Han (221BCE-220CE) empire, the northwest was a new territory to the Chinese realm. Until the Later Han (25-220CE) times, some portions of the northwestern region had only been part of imperial soil for one hundred years. Its coalescence into the Chinese empire was a product of long-term expansion and conquest, which arguably defined the egionr 's military nature. Furthermore, in the harsh natural environment of the region, only tough people could survive, and unsurprisingly, the region fostered vigorous warriors. Mixed culture and multi-ethnicity featured prominently in this highly militarized frontier society, which contrasted sharply with the imperial center that promoted unified cultural values and stood in the way of a greater degree of transregional integration. As this project shows, it was the northwesterners who went through a process of political peripheralization during the Later Han times played a harbinger role of the disintegration of the empire and eventually led to the breakdown of the early imperial system in Chinese history. -
The Ideology and Significance of the Legalists School and the School Of
Advances in Social Science, Education and Humanities Research, volume 351 4th International Conference on Modern Management, Education Technology and Social Science (MMETSS 2019) The Ideology and Significance of the Legalists School and the School of Diplomacy in the Warring States Period Chen Xirui The Affiliated High School to Hangzhou Normal University [email protected] Keywords: Warring States Period; Legalists; Strategists; Modern Economic and Political Activities Abstract: In the Warring States Period, the legalist theory was popular, and the style of reforming the country was permeated in the land of China. The Seven Warring States known as Qin, Qi, Chu, Yan, Han, Wei and Zhao have successively changed their laws and set the foundation for the country. The national strength hovers between the valley and school’s doctrines have accelerated the historical process of the Great Unification. The legalists laid a political foundation for the big country, constructed a power framework and formulated a complete policy. On the rule of law, the strategist further opened the gap between the powers of the country. In other words, the rule of law has created conditions for the cross-border family to seek the country and the activity of the latter has intensified the pursuit of the former. This has sparked the civilization to have a depth and breadth thinking of that period, where the need of ideology and research are crucial and necessary. This article will specifically address the background of the legalists, the background of these two generations, their historical facts and major achievements as well as the research into the practical theory that was studies during that period. -
On Confucius's Ideology of Aesthetic Order
Cultural Encounters, Conflicts, and Resolutions Volume 3 Issue 1 Article 6 12-2016 On Confucius’s Ideology of Aesthetic Order Li Wang Northeast Normal University, [email protected] Follow this and additional works at: https://engagedscholarship.csuohio.edu/cecr Part of the Bilingual, Multilingual, and Multicultural Education Commons, Critical and Cultural Studies Commons, Cultural History Commons, Dispute Resolution and Arbitration Commons, Gender, Race, Sexuality, and Ethnicity in Communication Commons, International and Intercultural Communication Commons, Peace and Conflict Studies Commons, and the Social and Cultural Anthropology Commons How does access to this work benefit ou?y Let us know! Recommended Citation Wang, Li (2016) "On Confucius’s Ideology of Aesthetic Order," Cultural Encounters, Conflicts, and Resolutions: Vol. 3 : Iss. 1 , Article 6. Available at: https://engagedscholarship.csuohio.edu/cecr/vol3/iss1/6 This Article is brought to you for free and open access by the World Languages, Literatures, and Cultures Journal at EngagedScholarship@CSU. It has been accepted for inclusion in Cultural Encounters, Conflicts, and Resolutions by an authorized editor of EngagedScholarship@CSU. For more information, please contact [email protected]. On Confucius’s Ideology of Aesthetic Order Abstract Advocating order, order for all things, and taking order as beauty is the core element of Confucius’s aesthetic ideology. Confucius’s thought of aesthetic order is different from others of the “hundred schools of thoughts” in the pre-Qin period, and is also diverse from the Western value of aesthetic order. Confucius’s thought of aesthetic order has its own unique value system, which has become the mainstream value of aesthetic order in the Chinese society for 2000 years until today, after being integrated with the Chinese feudal imperial system in early Han Dynasty. -
Where Was the Western Zhou Capital? a Capital City Has a Special Status in Every Country
Maria Khayutina [email protected] Where Was the Western Zhou Capital? A capital city has a special status in every country. Normally, this is a political, economical, social center. Often it is a cultural and religious center as well. This is the place of governmental headquarters and of the residence of power-holding elite and professional administrative cadres. In the societies, where transportation means are not much developed, this is at the same time the place, where producers of the top quality goods for elite consumption live and work. A country is often identified with its capital city both by its inhabitants and the foreigners. Wherefore, it is hardly possible to talk about the history of a certain state without making clear, where was located its capital. The Chinese history contains many examples, when a ruling dynasty moved its capital due to defensive or other political reasons. Often this shift caused not only geographical reorganization of the territory, but also significant changes in power relations within the state, as well as between it and its neighbors. One of the first such shifts happened in 771 BC, when the heir apparent of the murdered King You 幽 could not push back invading 犬戎 Quanrong hordes from the nowadays western 陜西 Shaanxi province, but fled to the city of 成周 Chengzhou near modern 洛陽 Luoyang, where the royal court stayed until the fall of the 周 Zhou in the late III century BC. This event is usually perceived as a benchmark between the two epochs – the “Western” and “Eastern” Zhou respectively, distinctly distinguished one from another. -
Modes of Membrane Interaction of a Natural Cysteine-Rich Peptide: Viscotoxin A3
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector Biochimica et Biophysica Acta 1559 (2002) 145^159 www.bba-direct.com Modes of membrane interaction of a natural cysteine-rich peptide: viscotoxin A3 Alexandre Coulon a, Emir Berkane a, Anne-Marie Sautereau a, Konrad Urech b, Pierre Rouge¨ a, Andre¨ Lopez a;* a Institut de Pharmacologie et de Biologie Structurale, UMR-CNRS 5089, 205 Route de Narbonne, 31077 Toulouse Cedex 4, France b Verein fu«r Krebsforschung, Hiscia Institut, CH 4144 Arlesheim, Switzerland Received 9 October 2001; received in revised form 15 November 2001; accepted 22 November 2001 Abstract Among the very homologous family of K- and L-thionins, known for their antimicrobial activity, the viscotoxin subfamily differs from other members because it is cytotoxic against tumoral cells but weakly hemolytic. We studied the interactions between the most active of these toxins, viscotoxin A3 (VA3), and model membranes made of phosphatidylcholine and phosphatidylserine (PS), the major zwitterionic and acidic phospholipids found in eukaryotic cells. Monolayer studies showed that electrostatic forces are essential for the interaction and are mainly involved in modulating the embedding of the toxin in the PS head group region. This in turn induces membrane stiffening, as shown by fluorescence polarization assays with 1,6-diphenyl-1,3,5-hexatriene and its derivatives. Moreover, vesicle permeabilization analyses showed that there are two modes of interaction, which are directly related to the stiffening effect and depend on the amount of VA3 bound to the surface of the vesicles. -
Becoming a Vessel Or Not an Analysis of Jun Zi Bu Qi 君子不器
The 3rd BESETO Conference of Philosophy Session 6 Becoming a Vessel or Not An Analysis of jun zi bu qi 君子不器 WU Ning Peking University Abstract The Analects focuses on the theme of how an exemplary person (jun zi 君子) should cultivate himself. In the Analects, the Master speaks of exemplary persons in so many situations that the reader easily obtains an impression of what constitutes an exem- plary person. However, one perplexing passage regarding the exemplary person is found in the Analects (2.12). Due to its extreme succinctness, the Master’s remark, jun zi bu qi 君子不器, which has been interpreted in many ways by ancient Chinese com- mentators and influential English translators, is enigmatic, not merely grammatically, but also literally. The principal aim of this essay is to make theAnalects 2.12 clear and try to offer a constructive interpretation of this passage. In order to show the meaning of jun zi bu qi as a positive attitude of Confucius, I outline the interpretations of some ancient Chinese commentaries and famous English translations, and then propose an interpretation of jun zi bu qi that yields a consistent reading of the Analects after a brief grammatical analysis. The conclusion of this paper is: as the perfect and complete manifestation of Confucian humanism, a jun zi should not be a mere specialist but rather a comprehensive vessel (qi) in li, for once a jun zi is such a specialist, then he is not worth the name of the exemplary person, and he is nothing but an ordinary utensil devoid of characteristics. -
Treatment Principles of Obesity with Chinese Herbal Medicine: Literature Analysis by Text Mining
Engineering, 2013, 5, 7-11 http://dx.doi.org/10.4236/eng.2013.510B002 Published Online October 2013 (http://www.scirp.org/journal/eng) Treatment Principles of Obesity with Chinese Herbal Medicine: Literature Analysis by Text Mining Yunyu Huang1*, Lianjie Wang1*, Shidong Wang1, Feng Cai2, Guang Zheng3, Aiping Lu2,4, Xiuchen Yu1#, Miao Jiang2# 1Beijing Dongzhimen Hospital affiliated to Beijing University of Chinese Medicine, Beijing, China 2Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing, China 3School of Information Science-Engineering, Lanzhou University, Lanzhou, China 4Hong Kong Baptist University School of Chinese Medicine, Kowloon, Hong Kong, China Email: #[email protected], #[email protected] Received September 2012 ABSTRACT Obesity represents a social health problem worldwide, associated with serious health risks and increased mortality. The prevalence of obesity is reported to be increasing in both developed and developing countries. Obesity is associated with a significant range of comorbidities and is linked with increases in mortality, thus the treatment of obesity is very important. Chinese herbal medicine (CHM) has been used for weight management both in China and in western coun- tries for many years, the effectiveness and safety of CHMs in obesity have been proved. Yet the principles of treating obesity with CHMs are hard to manage due to the complexity of TCM theory. In this study, a novel text mining method was developed based on a comprehensive collection of literatures in order to explore the treatment principles more in- tuitively. Networks of TCM patterns and CHMs which are most frequently used in obesity treatment are built-up and analyzed, two major principles are explored in treating obesity: one is resolving phlegm and dampness, the other is clearing heat and reinforcing deficiency. -
The Warring States Period (453-221)
Indiana University, History G380 – class text readings – Spring 2010 – R. Eno 2.1 THE WARRING STATES PERIOD (453-221) Introduction The Warring States period resembles the Spring and Autumn period in many ways. The multi-state structure of the Chinese cultural sphere continued as before, and most of the major states of the earlier period continued to play key roles. Warfare, as the name of the period implies, continued to be endemic, and the historical chronicles continue to read as a bewildering list of armed conflicts and shifting alliances. In fact, however, the Warring States period was one of dramatic social and political changes. Perhaps the most basic of these changes concerned the ways in which wars were fought. During the Spring and Autumn years, battles were conducted by small groups of chariot-driven patricians. Managing a two-wheeled vehicle over the often uncharted terrain of a battlefield while wielding bow and arrow or sword to deadly effect required years of training, and the number of men who were qualified to lead armies in this way was very limited. Each chariot was accompanied by a group of infantrymen, by rule seventy-two, but usually far fewer, probably closer to ten. Thus a large army in the field, with over a thousand chariots, might consist in total of ten or twenty thousand soldiers. With the population of the major states numbering several millions at this time, such a force could be raised with relative ease by the lords of such states. During the Warring States period, the situation was very different. -
The Zhou Dynasty Around 1046 BC, King Wu, the Leader of the Zhou
The Zhou Dynasty Around 1046 BC, King Wu, the leader of the Zhou (Chou), a subject people living in the west of the Chinese kingdom, overthrew the last king of the Shang Dynasty. King Wu died shortly after this victory, but his family, the Ji, would rule China for the next few centuries. Their dynasty is known as the Zhou Dynasty. The Mandate of Heaven After overthrowing the Shang Dynasty, the Zhou propagated a new concept known as the Mandate of Heaven. The Mandate of Heaven became the ideological basis of Zhou rule, and an important part of Chinese political philosophy for many centuries. The Mandate of Heaven explained why the Zhou kings had authority to rule China and why they were justified in deposing the Shang dynasty. The Mandate held that there could only be one legitimate ruler of China at one time, and that such a king reigned with the approval of heaven. A king could, however, loose the approval of heaven, which would result in that king being overthrown. Since the Shang kings had become immoral—because of their excessive drinking, luxuriant living, and cruelty— they had lost heaven’s approval of their rule. Thus the Zhou rebellion, according to the idea, took place with the approval of heaven, because heaven had removed supreme power from the Shang and bestowed it upon the Zhou. Western Zhou After his death, King Wu was succeeded by his son Cheng, but power remained in the hands of a regent, the Duke of Zhou. The Duke of Zhou defeated rebellions and established the Zhou Dynasty firmly in power at their capital of Fenghao on the Wei River (near modern-day Xi’an) in western China.