TFF1 Rabbit Polyclonal Antibody – TA322883 | Origene

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TFF1 Rabbit Polyclonal Antibody – TA322883 | Origene

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for TA322883 TFF1 Rabbit Polyclonal Antibody Product data: Product Type: Primary Antibodies Applications: IHC, WB Recommended Dilution: ELISA: 1:2000-5000, WB: 1:500-2000,IHC: 1:25-100 Reactivity: Human, Mouse, Rat Host: Rabbit Isotype: IgG Clonality: Polyclonal Immunogen: Fusion protein corresponding to a region derived from 25-84 amino acids of human trefoil factor 1 Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol Concentration: lot specific Purification: Antigen affinity purification Conjugation: Unconjugated Storage: Store at -20°C as received. Stability: Stable for 12 months from date of receipt. Predicted Protein Size: 9 kDa Gene Name: trefoil factor 1 Database Link: NP_003216 Entrez Gene 21784 MouseEntrez Gene 117270 RatEntrez Gene 7031 Human P04155 Background: Members of the trefoil family are characterized by having at least one copy of the trefoil motif; a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined; but they may protect the mucosa from insults; stabilize the mucus layer; and affect healing of the epithelium. This gene; which is expressed in the gastric mucosa; has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.? Synonyms: BCEI; D21S21; HP1.A; HPS2; pNR-2; pS2 This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 2 TFF1 Rabbit Polyclonal Antibody – TA322883 Protein Families: Druggable Genome, Secreted Protein Product images: Predicted band size: 9 kDa. Positive control: Mouse intestinum tenue tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%+12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Predicted cell location: Cytoplasm. Positive control: Human cervical cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin- embedded human cervical cancer tissue using TFF1 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x200) This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 2.

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Anti-Ps2 / Trefoil Factor 1 Antibody (ARG41084)
Product datasheet [email protected] ARG41084 Package: 100 μl anti-pS2 / Trefoil factor 1 antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes pS2 / Trefoil factor 1 Tested Reactivity Hu Tested Application FACS, ICC/IF, IHC-P, IP, WB Host Rabbit Clonality Polyclonal Isotype IgG Target Name pS2 / Trefoil factor 1 Antigen Species Human Immunogen Synthetic peptide derived from Human pS2 / Trefoil factor 1. Conjugation Un-conjugated Alternate Names D21S21; pNR-2; Trefoil factor 1; PNR-2; BCEI; hP1.A; HPS2; pS2; Polypeptide P1.A; HP1.A; Protein pS2; Breast cancer estrogen-inducible protein Application Instructions Application table Application Dilution FACS 1:50 ICC/IF 1:50 - 1:200 IHC-P 1:50 - 1:200 IP 1:50 WB 1:500 - 1:2000 Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Calculated Mw 9 kDa Observed Size 11 kDa Properties Form Liquid Purification Affinity purified. Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. Preservative 0.02% Sodium azide Stabilizer 50% Glycerol www.arigobio.com 1/2 Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. Note For laboratory research only, not for drug, diagnostic or other use. Bioinformation Gene Symbol TFF1 Gene Full Name trefoil factor 1 Background Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides.
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Location Analysis of Estrogen Receptor Target Promoters Reveals That
Location analysis of estrogen receptor ␣ target promoters reveals that FOXA1 defines a domain of the estrogen response Jose´ e Laganie` re*†, Genevie` ve Deblois*, Ce´ line Lefebvre*, Alain R. Bataille‡, François Robert‡, and Vincent Gigue` re*†§ *Molecular Oncology Group, Departments of Medicine and Oncology, McGill University Health Centre, Montreal, QC, Canada H3A 1A1; †Department of Biochemistry, McGill University, Montreal, QC, Canada H3G 1Y6; and ‡Laboratory of Chromatin and Genomic Expression, Institut de Recherches Cliniques de Montreál, Montreal, QC, Canada H2W 1R7 Communicated by Ronald M. Evans, The Salk Institute for Biological Studies, La Jolla, CA, July 1, 2005 (received for review June 3, 2005) Nuclear receptors can activate diverse biological pathways within general absence of large scale functional data linking these putative a target cell in response to their cognate ligands, but how this binding sites with gene expression in specific cell types. compartmentalization is achieved at the level of gene regulation is Recently, chromatin immunoprecipitation (ChIP) has been used poorly understood. We used a genome-wide analysis of promoter in combination with promoter or genomic DNA microarrays to occupancy by the estrogen receptor ␣ (ER␣) in MCF-7 cells to identify loci recognized by transcription factors in a genome-wide investigate the molecular mechanisms underlying the action of manner in mammalian cells (20–24). This technology, termed 17␤-estradiol (E2) in controlling the growth of breast cancer cells. ChIP-on-chip or location analysis, can therefore be used to deter- We identified 153 promoters bound by ER␣ in the presence of E2. mine the global gene expression program that characterize the Motif-finding algorithms demonstrated that the estrogen re- action of a nuclear receptor in response to its natural ligand.
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Molecular Alterations in the Stomach of Tffl-Deficient Mice
International Journal of Molecular Sciences Article Molecular Alterations in the Stomach of Tff1-Deficient Mice: Early Steps in Antral Carcinogenesis Eva B. Znalesniak, Franz Salm and Werner Hoffmann * Institute of Molecular Biology and Medicinal Chemistry, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120 Magdeburg, Germany; [email protected] (E.B.Z.) * Correspondence: werner.hoff[email protected] Received: 2 December 2019; Accepted: 14 January 2020; Published: 18 January 2020 Abstract: TFF1 is a peptide of the gastric mucosa co-secreted with the mucin MUC5AC. It plays a key role in gastric mucosal protection and repair. Tff1-deficient (Tff1KO) mice obligatorily develop antropyloric adenoma and about 30% progress to carcinomas. Thus, these mice represent a model for gastric tumorigenesis. Here, we compared the expression of selected genes in Tff1KO mice and the corresponding wild-type animals (RT-PCR analyses). Furthermore, we systematically investigated the different molecular forms of Tff1 and its heterodimer partner gastrokine-2 (Gkn2) in the stomach (Western blot analyses). As a hallmark, a large portion of murine Tff1 occurs in a monomeric form. This is unexpected because of its odd number of seven cysteine residues. Probably the three conserved acid amino acid residues (EEE) flanking the 7th cysteine residue allow monomeric secretion. As a consequence, the free thiol of monomeric Tff1 could have a protective scavenger function, e.g., for reactive oxygen/nitrogen species. Furthermore, a minor subset of Tff1 forms a disulfide-linked heterodimer with IgG Fc binding protein (Fcgbp). Of special note, in Tff1KO animals a homodimeric form of Gkn2 was observed. In addition, Tff1KO animals showed strongly reduced Tff2 transcript and protein levels, which might explain their increased sensitivity to Helicobacter pylori infection.
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Appendix 2. Significantly Differentially Regulated Genes in Term Compared with Second Trimester Amniotic Fluid Supernatant
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International Journal of Molecular Sciences Review Trefoil Factor Family (TFF) Peptides and Their Diverse Molecular Functions in Mucus Barrier Protection and More: Changing the Paradigm Werner Hoffmann Institute ofReview Molecular Biology and Medicinal Chemistry, Otto-von-Guericke University Magdeburg, LeipzigerT Str.refoil 44, 39120 Factor Magdeburg, Family Germany; (TFF werner.ho) Peptidesff[email protected] and Their Received:Diverse 19 May 2020; Molecular Accepted: 19 June Functions 2020; Published: in 25 JuneMucus 2020 Barrier Abstract:ProtectionTrefoil factor family and peptidesMore: (TFF1,Chang TFF2,ing TFF3) the are Paradigm typically co-secreted together with mucins. Tff1 represents a gastric tumor suppressor gene in mice. TFFs are also synthesized in minute Werner Hoffmann amounts in the immune and central nervous systems. In mucous epithelia, they support rapid repair byInstitute enhancing of Molecul cellar migrationBiology and Medicinal (“restitution”) Chemistry, Otto via-von their-Guericke weak University chemotactic Magdeburg and, anti-apoptotic Leipziger Str. 44, 39120 Magdeburg, Germany; [email protected] effects. For a long time, as a paradigm, this was considered as their major biological function. Within Received: 19 May 2020; Accepted: 19 June; Published: 25 June 2020 recent years, the formation of disulfide-linked heterodimers was documented for TFF1 and TFF3, e.g., withAbstract: gastrokine-2 Trefoil andfactor IgGfamily Fc peptides binding (TFF1, protein TFF2, TFF3) (FCGBP). are typically Furthermore, co-secreted lectintogether activities with were recognizedmucins as enabling. Tff1 represents binding a gastric to atumor lipopolysaccharide suppressor gene in mice of .Helicobacter TFFs are also synthesized pylori (TFF1, in minute TFF3) or to a amounts in the immune and central nervous systems.
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Down Syndrome Congenital Heart Disease: a Narrowed Region and a Candidate Gene Gillian M
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(12) Patent Application Publication (10) Pub. No.: US 2004/0191783 A1 Leclercq Et Al
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