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Gyrothrix Oleae Fungal Planet Description Sheets 305

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Gyrothrix Oleae Fungal Planet Description Sheets 305 304 Persoonia – Volume 43, 2019 Gyrothrix oleae Fungal Planet description sheets 305 Fungal Planet 983 – 18 December 2019 Gyrothrix oleae Crous, sp. nov. Etymology. Name refers to the host genus Olea from which it was isolated. Notes — The hyphomycete genus Gyrothrix closely resem- bles Circinotrichum (see FP960). Gyrothrix oleae is closely Classification — Incertae sedis, Xylariales, Sordariomycetes. related to Circinotrichum papakurae (setae unbranched, conidia Mycelium consisting of hyaline, smooth, branched, septate, 11–17 × 1.5–2 µm; Hughes & Pirozynski 1971) and Gyrothrix 2–3 µm diam hyphae. Setae erect, 100–150 µm long, 3–4 µm ramosa (setae branched, conidia 14–19 × 2–2.7 µm; Zucconi diam, brown, multiseptate, thick-walled, verruculose to warty, & Onofri 1989), but can be distinguished based on its smaller subcylindrical with apical taper, base bulbous, 4–6 µm diam, conidia. apex spirally curved, apical region frequently with curved lateral Based on a megablast search of NCBIs GenBank nucleotide branches. Conidiophores reduced to conidiogenous cells ar- database, the closest hits using the ITS sequence of CPC 37069 ranged around the base of setae, subcylindrical to ampulliform, had highest similarity to Ascotricha pusilla (strain CBS 132.60, hyaline to subhyaline, smooth, 7–13 × 2–3 µm, proliferating GenBank MH857921.1; Identities = 506/592 (85 %), 29 gaps percurrently at apex. Conidia hyaline, smooth, aseptate, fu- (4 %)), Xylaria liquidambaris (voucher HMJAU 22124, GenBank soid, inequilateral, inner plane flat, outer plane convex, apex JX256826.1; Identities = 506/597 (85 %), 33 gaps (5 %)), and subobtuse, tapering toward inner plane, base with excentric, Virgaria boninensis (strain JCM 18622, GenBank AB670709.1; truncate hilum, tapering towards inner plane, (7–)9–10(–11) Identities = 395/439 (90 %), 15 gaps (3 %)). The ITS sequen- × (1.5–)2 µm. ces of CPC 37063 and 37069 differ with a single nucleotide Culture characteristics — Colonies flat, spreading, with (581/582 bases similar). Closest hits using the LSU sequence of moderate aerial mycelium and smooth, lobate margin, reaching CPC 37069 are Circinotrichum papakurae (strain CBS 101373, 25 mm diam after 2 wk at 25 °C. On MEA and PDA surface and GenBank KR611897.1; Identities = 819/840 (98 %), 2 gaps (0 %)), reverse mouse grey. On OA surface pale purplish grey. Gyrothrix ramosa (strain MUCL 54061, GenBank KC775722.1; Typus. SOUTH AFRICA, Western Cape Province, Knysna, Knysna area, Identities = 781/802 (97 %), 3 gaps (0 %)), and Gyrothrix inops on leaves of Olea capensis subsp. macrocarpa (Oleaceae), 22 Nov. 2018, (strain BE108, GenBank KC775721.1; Identities = 790/814 F. Roets, HPC 2728 (holotype CBS H-24184, culture ex-type CPC 37069 = CBS (97 %), 6 gaps (0 %)). The LSU sequences of CPC 37063 and 146069, ITS and LSU sequences GenBank MN562136.1 and MN567643.1, 37069 differ with a single nucleotide (837/838 bases similar). MycoBank MB832895). Additional material examined. SOUTH AFRICA, Western Cape Province, Knysna, Knysna area, on Diospyros whyteana (Ebenaceae), 22 Nov. 2018, F. Roets, HPC 2720, culture CPC 37063 = CBS 146068, ITS and LSU se- quences GenBank MN562137.1 and MN567644.1. Colour illustrations. Olea capensis subsp. macrocarpa trees in Knysna forest. Conidiogenous cells; conidia; setae. Scale bars = 10 µm. Pedro W. Crous & Johannes Z. Groenewald, Westerdijk Fungal Biodiversity Institute, P.O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: [email protected] & [email protected] Michael J. Wingfield, Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, Private Bag X20, Hatfield 0028, Pretoria, South Africa; e-mail: [email protected] Francois Roets, Department of Conservation Ecology and Entomology, Stellenbosch University, Stellenbosch 7600, South Africa; e-mail: [email protected] Wijnand J. Swart, Department of Plant Sciences (Division of Plant Pathology), University of the Free State, P.O. Box 339, Bloemfontein 9300, South Africa; e-mail: [email protected] © 2019 Naturalis Biodiversity Center & Westerdijk Fungal Biodiversity Institute.
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