Second-Generation Electron Transfer Dissociation (ETD) on the Thermo
Second-Generation Electron Transfer Dissociation (ETD) on the Thermo Scientifi c Orbitrap Fusion Mass Spectrometer with Improved Functionality, Increased Speed, and Improved Robustness of Data Christopher Mullen,1 Lee Earley,1 Jean-Jacques Dunyach,1 John E.P. Syka,1 Philip D. Compton,2 Dina L. Bai,3 Jefferey Shabanowitz,3 and Donald F. Hunt3 1Thermo Fisher Scientifi c, San Jose, CA; 2Kelleher Lab, Northwestern University, Evanston, IL; 3Department of Chemistry, University of Virginia, Charlottesville, VA Second-Generation Electron Transfer Dissociation (ETD) on the Thermo Scientific Orbitrap Fusion Mass Spectrometer with Improved Functionality, Increased Speed, and Improved Robustness of Data Christopher Mullen1, Lee Earley1, Jean-Jacques Dunyach1, John E.P. Syka1, Philip D. Compton2, Dina L. Bai3, Jefferey Shabanowitz3, and Donald F. Hunt3 1Thermo Fisher Scientific, San Jose, CA; 2Kelleher Lab, Northwestern University, Evanston, IL; 3Department of Chemistry, University of Virginia, Charlottesville, VA TABLE 1. Calculated charge state dependent reaction times based on a Verification of the Calibration Unique ETD Capabilities Overview Results saturated reaction rate coefficient of 58.2 sec–1 for charge state 3+, based on 5% The calibrated ETD reaction conditions are verified by using an infusion of ubiquitin The location of the ion-routing multipole within the Orbitrap Fusion mass spectrometer of the precursor remaining after reaction. Purpose: Improve ETD robustness, functionality, and speed on the Thermo Calibrating the reaction kinetics ensures that the ETD fragmentation efficiency is from bovine erythrocytes. Spectra of the 12+ charge state at 714.7 m/z were obtained allows for a parallel ITMS2 acquisition mode, enabling ITMS2 CID and HCD spectral Scientific™ Orbitrap Fusion™ Tribrid™ mass spectrometer optimized and that the maximum duty cycle for ETD can be accomplished.
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