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Orbitrap Elite Hybrid MS Provides the Ultimate Level of Versatility and Performance for Your Research

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Orbitrap Elite Hybrid MS Provides the Ultimate Level of Versatility and Performance for Your Research mass spectrometry Product Specifications Combining the next generation Thermo Scientific Velos Pro ion trap with the new high-field Orbitrap technology, the Thermo Scientific Orbitrap Elite hybrid MS provides the ultimate level of versatility and performance for your research. Thermo Scientific Orbitrap Elite High-Field Orbitrap Hybrid Mass Spectrometer • Novel high-field Orbitrap analyzer The ultrahigh resolution provides • Resolving power > 240,000 FWHM certainty in analytical results by enabling • Advanced signal processing molecular weight determination for intact • Multiple fragmentation techniques: CID, proteins and in-depth analysis of isobaric HCD and optionally ETD species. The unsurpassed resolution of the • Parallel MS and MSn analysis system is especially useful when dealing • Velos Pro ion trap generation II ion optics with complex and low abundance samples, with neutral blocking technology increase in applications such as proteomics, metabo- robustness lomics and lipidomics. The Orbitrap Elite hybrid MS can The Thermo Scientific Orbitrap Elite MS is perform a wide variety of experiments from the industry-leading high performance in-depth discovery based experiments to hybrid mass spectrometer combining the comprehensive qual/quan experiments. The novel high-field Orbitrap™ analyzer with availability of multiple fragmentation tech- the latest dual-pressure linear ion trap, the niques (CID, HCD and optional ETD) offers Thermo Scientific Velos Pro. a new level of versatility and performance The high-field Orbitrap analyzer for the most challenging research applica- increases speed, sensitivity and dynamic tions. range to dig deeper into your samples. Part of Thermo Fisher Scientific Product Specifications Hardware Specifications Velos Pro Dual-Pressure Orbitrap Mass Analyzer Performance Characteristics Linear Trap • Gas-free multipole ion transfer optics • Gas (nitrogen) filled C-Trap Ion Max™ API Source Mass Range m/z 50 - 2,000, m/z 200 - 4,000 • High-transmission ion transfer optics • Enhanced sensitivity and ruggedness Resolution 60,000 at m/z 400 at a scan • Straight multipole collision cell with axial • Sweep Gas™ reduces chemical noise (FWHM) rate of 4 Hz, field for HCD minimum resolution 15,000, • 60° interchangeable ion probe orientation • Next generation high-field Orbitrap maximum resolution • Removable metal ion transfer tube analyzer > 240,000 at m/z 400 provides vent-free maintenance • Active temperature control using a Peltier Mass Accuracy < 3 ppm RMS element with external calibration S-Lens • Differentially pumped vacuum system < 1 ppm RMS • A progressively spaced stacked ring ion with: using internal calibration guide (S-lens) - Rotary vane pumps as fore-vacuum pumps, MS/MS Electrospray Ionization (ESI): • The S-lens is a radio frequency (RF) device three water-cooled 60 L turbomolecular Sensitivity 2 μL of a 50 fg/μL solution of that captures and efficiently focuses the pumps, and one water-cooled 210 L of Ion Trap reserpine (100 femtograms ions in a tight beam turbomolecular pump total) injected at a flow of • Large variable spacing between - Final vacuum under operating conditions 500 μL/min will produce electrodes allows for better pumping < 2 x 10-10 Torr a minimum signal-to-noise ratio efficiency and improves ruggedness - Vacuum control by active Pirani gauge and of 100:1 for the transition of the isolated protonated • Automatic tuning program for optimizing cold ion gauge molecular ions at m/z 609 to transmission • New low noise detect amplifier the largest two product ions, • 14-bit signal digitization m/z 397 and m/z 448, when Transfer Ion Optics • Ultra-fast real-time data acquisition and the mass spectrometer is • Generation II ion optics with neutral block- instrument control system operated at unit resolution in ing technology for improved robustness • Automatic calibration of all ion transfer the full scan MS/MS mode, • High stability and ion transmission scanning the product ion and Orbitrap parameters via instrument [1] efficiency dual-pressure linear ion trap control software spectrum from m/z 165-615. • High-pressure cell for improved precursor Dynamic Range > 5,000 within a single scan ion trapping, isolation and fragmentation guaranteeing specified efficiency Options mass accuracy • Low-pressure cell for improved scan • H-ESI II – Heated Electrospray Ionization MS Scan Power MSn, for n = 1 through 10 speed, resolving power and mass Source for enhanced ionization efficiency accuracy with flow rates of 1 μL/min up to ETD Option 3 μL/min infusion of < 2,000 μL/min (required) a 1 pmol/μL solution of Angiotensin I will produce an • ESI probe compatible with liquid flow Vacuum System electron transfer dissociation rates of < 1 μL/min to 1 mL/min without • Differentially pumped vacuum system fragmentation efficiency -5 splitting to 10 Torr of > 15% • Split-flow turbomolecular pump control- • New NanoSpray Flex Ion Source supports Analog Inputs One (1) analog input (0 - 1 V), ling vacuum in three regions static packed tip and dynamic nanospray One (1) analog (0 - 10 V) • Dual rotary vacuum pump configuration experiments, compatible with liquid flow rates of 50 nL/min to 2 μL/min. Lower [1] Requires H-ESI II • Aluminum high-vacuum analyzer chambers limit is dependent on gauge of needle • Novel electron multipliers with higher used linear dynamic range for improved quanti- • APCI/APPI source compatible with liquid tation capabilities flow rates of 50 μL/min to 2 mL/min • Digital electronic noise discrimination without splitting • Metal needle option for high- and low-flow analyses Schematic of the Orbitrap Elite Hybrid MS Square Electrospray Quadrupole with High-Pressure Low- Quadrupole Ion Source S-Lens Neutral Blocker Octopole Cell Pressure Cell Mass Filter C-Trap HCD Collision Cell Transfer Multipole Reagent Ion Source e- New Detection Electronics New High-Field Orbitrap Mass Analyzer Reagent 1 Reagent 2 Heated Inlet Heated Inlet ETD Option Software Features Data System Exclusive Technologies Advanced Data Dependent • High performance PC with Intel® • Precursor ion selection in the linear Experiments microprocessor ion trap and fragmentation in the new • Data Dependent features trigger n • High resolution LCD color monitor collision cell (HCD) with high resolution acquisition of MS spectra only when accurate mass detection in the Orbitrap a compound of interest is detected • Microsoft Windows® 7 operating system mass analyzer • Isotopic Data Dependent scanning soft- • Microsoft Office 2010 software package • Pulsed-Q Collision Induced Dissociation ware triggers MS/MS scanning only when • Thermo Scientific Xcalibur processing and (PQD) enables trapping of low mass a specified isotopic pattern is detected instrument control software fragment ions • Ion Mapping automatically generates a • FT-Programs software tools: Protein • Unique, patented* Automatic Gain Control three-dimensional MS/MS map, yielding Calculator and Recalibrate Offline (AGC™) ensures that the ion trap is always product ions, precursor ions, and neutral filled with the optimum number of ions for loss information any scan type Operation Modes • Ion Mapping Browser software displays • Dynamic Exclusion allows acquisition data generated by Ion Mapping experi- • Data Dependent Decision Tree – of MS/MS and MSn spectra from lower ments Automatic selection of the optimal intensity ion species n fragmentation technique based on peptide • Data Dependent Ion Tree performs MS ™ properties (charge state, m/z) for highest • WideBand Activation generates more experiments on up to 25 species fragmentation efficiency structurally informative spectra • MSn Browser software displays data ™ • High resolution accurate mass scans at • Normalized Collision Energy for both generated by Data Dependent Ion Tree high repetition rates CID and HCD fragmentation techniques and Ion Mapping experiments providing reproducible data from • Monoisotopic precursor selection for • Precursor ion isolation and instrument to instrument fragmentation in the linear ion trap (CID) Data Dependent MS/MS experiments with high resolution accurate mass • Stepped Normalized Collision Energy (CID • Data Dependent (accurate) Neutral Loss MS/MS and MSn data acquisition in the and HCD) allows for the variation of the - Trigger MS3 scans on only the MS/MS Orbitrap mass analyzer collision energies in MS/MS experiments product ions with a pre-defined neutral • Higher collision energy dissociation (HCD) • Multistage Activation (MSA) generates loss 3 for triple-quadrupole like fragmentation combination of MS/MS spectra and MS spectra based on a user defined neutral • Data Dependent™ scans using both the 46642.586 loss 100 linear ion trap and the Orbitrap mass analyzer 80 • Data Dependent MS/MS with parallel 993.9770 n R=110221 acquisition of multiple MS scans in the 100 994.0401 Charge State 47+ linear ion trap while acquiring a high R=107426 60 90 993.9134 resolution full scan MS spectrum in the R=109564 80 994.0827 Orbitrap analyzer R=107785 40 70 993.8706 • Ion Mapping, Neutral Loss Ion Mapping, R=107582 Parent Ion Mapping, user selectable 60 994.1249 R=106063 20 ™ 993.8284 Dynamic Exclusion , Nth order Triple Play Relative Abundance [%] 50 R=113310 994.1670 R=107915 experiment, Data Dependent Ion Tree 993.7860 0 experiment, Total Ion Map experiment 40 R=106257 46620 46650 Relative Abundance [%] 30 Mass, Da 20 Optional Application-Specific Software 10 0 • Thermo Scientific Proteome Discoverer 993.4 993.6 993.8 994.0 994.2 994.4 994.6 994.8 – Mass informatics platform
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