mass spectrometry Product Specifications Combining the next generation Thermo Scientific Velos Pro ion trap with the new high-field Orbitrap technology, the Thermo Scientific Orbitrap Elite hybrid MS provides the ultimate level of versatility and performance for your research. Thermo Scientific Orbitrap Elite High-Field Orbitrap Hybrid Mass Spectrometer • Novel high-field Orbitrap analyzer The ultrahigh resolution provides • Resolving power > 240,000 FWHM certainty in analytical results by enabling • Advanced signal processing molecular weight determination for intact • Multiple fragmentation techniques: CID, proteins and in-depth analysis of isobaric HCD and optionally ETD species. The unsurpassed resolution of the • Parallel MS and MSn analysis system is especially useful when dealing • Velos Pro ion trap generation II ion optics with complex and low abundance samples, with neutral blocking technology increase in applications such as proteomics, metabo- robustness lomics and lipidomics. The Orbitrap Elite hybrid MS can The Thermo Scientific Orbitrap Elite MS is perform a wide variety of experiments from the industry-leading high performance in-depth discovery based experiments to hybrid mass spectrometer combining the comprehensive qual/quan experiments. The novel high-field Orbitrap™ analyzer with availability of multiple fragmentation tech- the latest dual-pressure linear ion trap, the niques (CID, HCD and optional ETD) offers Thermo Scientific Velos Pro. a new level of versatility and performance The high-field Orbitrap analyzer for the most challenging research applica- increases speed, sensitivity and dynamic tions. range to dig deeper into your samples. Part of Thermo Fisher Scientific Product Specifications Hardware Specifications Velos Pro Dual-Pressure Orbitrap Mass Analyzer Performance Characteristics Linear Trap • Gas-free multipole ion transfer optics • Gas (nitrogen) filled C-Trap Ion Max™ API Source Mass Range m/z 50 - 2,000, m/z 200 - 4,000 • High-transmission ion transfer optics • Enhanced sensitivity and ruggedness Resolution 60,000 at m/z 400 at a scan • Straight multipole collision cell with axial • Sweep Gas™ reduces chemical noise (FWHM) rate of 4 Hz, field for HCD minimum resolution 15,000, • 60° interchangeable ion probe orientation • Next generation high-field Orbitrap maximum resolution • Removable metal ion transfer tube analyzer > 240,000 at m/z 400 provides vent-free maintenance • Active temperature control using a Peltier Mass Accuracy < 3 ppm RMS element with external calibration S-Lens • Differentially pumped vacuum system < 1 ppm RMS • A progressively spaced stacked ring ion with: using internal calibration guide (S-lens) - Rotary vane pumps as fore-vacuum pumps, MS/MS Electrospray Ionization (ESI): • The S-lens is a radio frequency (RF) device three water-cooled 60 L turbomolecular Sensitivity 2 μL of a 50 fg/μL solution of that captures and efficiently focuses the pumps, and one water-cooled 210 L of Ion Trap reserpine (100 femtograms ions in a tight beam turbomolecular pump total) injected at a flow of • Large variable spacing between - Final vacuum under operating conditions 500 μL/min will produce electrodes allows for better pumping < 2 x 10-10 Torr a minimum signal-to-noise ratio efficiency and improves ruggedness - Vacuum control by active Pirani gauge and of 100:1 for the transition of the isolated protonated • Automatic tuning program for optimizing cold ion gauge molecular ions at m/z 609 to transmission • New low noise detect amplifier the largest two product ions, • 14-bit signal digitization m/z 397 and m/z 448, when Transfer Ion Optics • Ultra-fast real-time data acquisition and the mass spectrometer is • Generation II ion optics with neutral block- instrument control system operated at unit resolution in ing technology for improved robustness • Automatic calibration of all ion transfer the full scan MS/MS mode, • High stability and ion transmission scanning the product ion and Orbitrap parameters via instrument [1] efficiency dual-pressure linear ion trap control software spectrum from m/z 165-615. • High-pressure cell for improved precursor Dynamic Range > 5,000 within a single scan ion trapping, isolation and fragmentation guaranteeing specified efficiency Options mass accuracy • Low-pressure cell for improved scan • H-ESI II – Heated Electrospray Ionization MS Scan Power MSn, for n = 1 through 10 speed, resolving power and mass Source for enhanced ionization efficiency accuracy with flow rates of 1 μL/min up to ETD Option 3 μL/min infusion of < 2,000 μL/min (required) a 1 pmol/μL solution of Angiotensin I will produce an • ESI probe compatible with liquid flow Vacuum System electron transfer dissociation rates of < 1 μL/min to 1 mL/min without • Differentially pumped vacuum system fragmentation efficiency -5 splitting to 10 Torr of > 15% • Split-flow turbomolecular pump control- • New NanoSpray Flex Ion Source supports Analog Inputs One (1) analog input (0 - 1 V), ling vacuum in three regions static packed tip and dynamic nanospray One (1) analog (0 - 10 V) • Dual rotary vacuum pump configuration experiments, compatible with liquid flow rates of 50 nL/min to 2 μL/min. Lower [1] Requires H-ESI II • Aluminum high-vacuum analyzer chambers limit is dependent on gauge of needle • Novel electron multipliers with higher used linear dynamic range for improved quanti- • APCI/APPI source compatible with liquid tation capabilities flow rates of 50 μL/min to 2 mL/min • Digital electronic noise discrimination without splitting • Metal needle option for high- and low-flow analyses Schematic of the Orbitrap Elite Hybrid MS Square Electrospray Quadrupole with High-Pressure Low- Quadrupole Ion Source S-Lens Neutral Blocker Octopole Cell Pressure Cell Mass Filter C-Trap HCD Collision Cell Transfer Multipole Reagent Ion Source e- New Detection Electronics New High-Field Orbitrap Mass Analyzer Reagent 1 Reagent 2 Heated Inlet Heated Inlet ETD Option Software Features Data System Exclusive Technologies Advanced Data Dependent • High performance PC with Intel® • Precursor ion selection in the linear Experiments microprocessor ion trap and fragmentation in the new • Data Dependent features trigger n • High resolution LCD color monitor collision cell (HCD) with high resolution acquisition of MS spectra only when accurate mass detection in the Orbitrap a compound of interest is detected • Microsoft Windows® 7 operating system mass analyzer • Isotopic Data Dependent scanning soft- • Microsoft Office 2010 software package • Pulsed-Q Collision Induced Dissociation ware triggers MS/MS scanning only when • Thermo Scientific Xcalibur processing and (PQD) enables trapping of low mass a specified isotopic pattern is detected instrument control software fragment ions • Ion Mapping automatically generates a • FT-Programs software tools: Protein • Unique, patented* Automatic Gain Control three-dimensional MS/MS map, yielding Calculator and Recalibrate Offline (AGC™) ensures that the ion trap is always product ions, precursor ions, and neutral filled with the optimum number of ions for loss information any scan type Operation Modes • Ion Mapping Browser software displays • Dynamic Exclusion allows acquisition data generated by Ion Mapping experi- • Data Dependent Decision Tree – of MS/MS and MSn spectra from lower ments Automatic selection of the optimal intensity ion species n fragmentation technique based on peptide • Data Dependent Ion Tree performs MS ™ properties (charge state, m/z) for highest • WideBand Activation generates more experiments on up to 25 species fragmentation efficiency structurally informative spectra • MSn Browser software displays data ™ • High resolution accurate mass scans at • Normalized Collision Energy for both generated by Data Dependent Ion Tree high repetition rates CID and HCD fragmentation techniques and Ion Mapping experiments providing reproducible data from • Monoisotopic precursor selection for • Precursor ion isolation and instrument to instrument fragmentation in the linear ion trap (CID) Data Dependent MS/MS experiments with high resolution accurate mass • Stepped Normalized Collision Energy (CID • Data Dependent (accurate) Neutral Loss MS/MS and MSn data acquisition in the and HCD) allows for the variation of the - Trigger MS3 scans on only the MS/MS Orbitrap mass analyzer collision energies in MS/MS experiments product ions with a pre-defined neutral • Higher collision energy dissociation (HCD) • Multistage Activation (MSA) generates loss 3 for triple-quadrupole like fragmentation combination of MS/MS spectra and MS spectra based on a user defined neutral • Data Dependent™ scans using both the 46642.586 loss 100 linear ion trap and the Orbitrap mass analyzer 80 • Data Dependent MS/MS with parallel 993.9770 n R=110221 acquisition of multiple MS scans in the 100 994.0401 Charge State 47+ linear ion trap while acquiring a high R=107426 60 90 993.9134 resolution full scan MS spectrum in the R=109564 80 994.0827 Orbitrap analyzer R=107785 40 70 993.8706 • Ion Mapping, Neutral Loss Ion Mapping, R=107582 Parent Ion Mapping, user selectable 60 994.1249 R=106063 20 ™ 993.8284 Dynamic Exclusion , Nth order Triple Play Relative Abundance [%] 50 R=113310 994.1670 R=107915 experiment, Data Dependent Ion Tree 993.7860 0 experiment, Total Ion Map experiment 40 R=106257 46620 46650 Relative Abundance [%] 30 Mass, Da 20 Optional Application-Specific Software 10 0 • Thermo Scientific Proteome Discoverer 993.4 993.6 993.8 994.0 994.2 994.4 994.6 994.8 – Mass informatics platform