sign in sign up
<<
Home , Eldana, Fall armyworm

Overview of Fall Armyworm, frugiperda

Jaspreet Sidhu Rangaswamy Muniappan

Virginia Tech FALL ARMY WORM (Spodoptera )

• Genus Spodoptera was described by Guenee in 1852 • Genera - Spodoptera, Laphygma and Prodenia - Synonimized • All three combined to form Spodoptera (Bayer,1960) • 25 species are in this genus. FALL ARMY WORM TAXONOMY (Spdoptera frugiperda)

• First described in 1797 as Phaleana frugiperda • In 1852, frugiperda was placed in genus Laphygma • In 1958 Laphygma and spodoptera were synonymized Different species in the Genus and their common names

• S. eridania :Southern armyworm • S. exigua : Beet armyworm • S. frugiperda : Fall armyworm Prevalent in U.S • S. ornithogalli : Yellowstriped armyworm • S. praefica : Western yellowstriped armyworm

• S. exempta : Nutgrass armyworm • S. littoralis : Egyptian leafworm • S. litura : Taro caterpillar • S. mauritia : Lawn armyworm Current distribution of economically important species

• S. frugiperda: U.S, South America, • S. littoralis : Africa, southern Europe, Western Arabian Peninsula, Islands of Indian ocean, Islands of Atlantic ocean • S. exempta : Africa, Australia, Hawaii, Western Arabian Peninsula • S. litura : Australia, Pacific Islands, Asia • S. mauritia : Madagascar, Saudi Arabia, Asia, Pacific Islands, Hawaii • S. Exigua: Africa, Western Arabian peninsula, Islands of Indian ocean, Islands of Atlantic ocean Current distribution of economically minor important species

• S. pectin: Asia • S. ochrea : Peru • S. marima : Brazil • S. cilium : Africa, Western Arabian Peninsula, Islands of Indian Ocean • S. triturata : Africa

Host Plants for each species

Species Plants S. exempta and Cyperacease S. littoralis 44 families including Leguminosae, Solanaceae, Malvaceae, Moraceae, Asteraceae, Poaceae, Chenopodiaceae, and Cruciferae. S. litura Over 100 hosts, including crucifers, legumes, millets, deciduous fruit trees, and various ornamentals and vegetables. S. mauritia Poaceae, , and Typhaceae S. pectin Poaceae and Cyperaceae S. frugiperda Over 100 host plants but prefers grasses. Spodoptera frugiperda Host Plants

• More than 100 plant species • , , , , cabbage, beet, , soybean,alfalfa, onion, cotton, pasture grasses, millet, tomato, and potato. • One of most damaging pests in the Americas. • In Brazil, annual loss - $400 million.

Fall army worm strains

• Corn Strain: Maize and sorghum • Rice strain: Rice and turfgrass • Morphologically identical • Distinguished by molecular markers: - Allozyme polymorphisms - Genetic polymorphisms - Mitochondrial haplotyping • Molecular differences are consistent with genetically distinct populations • Differ in susceptibility to chemical and biological agents Maize/ Corn Strain

• Florida haplotype profile – Florida and Caribbean population • Texas haplotype profile – Texas through Central America to Argentina

• Florida haplotype migrates north up to east of the Appalachian mountains every summer • Texas haplotype migrates north up to Canada west of the Appalachian mountains every summer Fall Armyworm U.S. Infestation and zone

Modified from R. Nagoshi ppt Fall Armyworm U.S. migration

Modified from R. Nagoshi ppt Maize and Rice Strains

• Both strains occur in Africa • Both strains seem to spread together • Florida haplotype was identified in • Both strains attack maize in Africa INTRODUCTION TO AFRICA (Matthew Cock et al 2017)

• It is intercontinental introduction • Might have happened as eggs, larvae, pupae or adults or combinations of these • Possible pathways of introduction: Unaided dispersal ־ Contaminant of a commodity ־ Stowaway on a vector ־ DISPERSAL (Matthew Cock et al 2017)

• Unaided dispersal Strong flier, Prevailing trade winds from Americas to Africa do not favor • Contaminant of a commodity • European Union interceptions 2012 – 2016 -17 on Capsicum peppers -11 on other Solanum spp. - 8 on other parts of plants • Minimal trade in fresh produce between Americas and Africa indicates chances of transmission through this route is small DISPERSAL (Matthew Cock et al 2017)

• Stowaway on a direct flight More likely

• In a 1950 study, of 9000 aircraft from South America and Caribbean to Miami, 98 had lepidopteran eggs

• Pre-oviposition female could be carried in cargo holds or wheel bays SPREAD WITHIN AFRICA

• Unaided dispersal by flight • Through contaminant • Were there multiple introductions? Biology of S. frugiperda Egg mass

• Eggs are dome shaped

• Dirty white to gray in color.

• Laid in groups of about 10-200 eggs per egg mass

• Eggs may be laid in a single or in two or more layers progressively superimposed on each other Egg mass

• Mostly laid on lower surface of the leaves

• Eggs are covered by a layer of grayish scales or hairs giving it a hairy or moldy appearance.

• Eggs hatch in about three to five days in summer. • Six larval • Fully-grown larvae are 3.1 – 3.8 cm long • Vary in color from pale green to almost black • Three yellowish stripes running down the back • Wider dark stripe and a wavy yellow-red blotched stripe on each side • Predominant white, inverted Y-shaped suture on the head • Larval duration is about 14 days during the summer and 30 days during cool weather Larval Behavior • Initially, newly hatched larvae feed near where the egg mass was laid. • Later, they move up onto the maize plants and consume leaf tissue excluding the veins and midrib. • The larvae exhibit cannibalistic behavior. • Under heavy infestations, the larval densities are often reduced to one or two per plant • Does not undergo • Killed by winter weather

• Pupation normally takes place in the soil at a depth of 2-8 cm.

• The pupa is reddish brown in color.

• Pupal duration is about eight to nine days during the summer, but may be longer than two weeks under winter conditions

• Does not diapause over winter Adult • The adult moths have a wingspan of 32 to 40 mm

• Males have dark gray and brown shaded mottled forewings with conspicuous triangular white spots at the tip and near the center of the wing

• The hind wing is iridescent silver-white with a narrow dark border in both sexes

• Adults are nocturnal, and are most active at dusk for mating

• Adults can live up to an average of 10 days but sometimes the duration extends up to three weeks Damage on Maize

• Prefers young maize plants • Its attacks all stages of maize plant, foliar consumption is the major factor • Generally feeds on foliage, but during heavy infestations, also feed on maize ears • Foliar damage characterized by ragged feeding, and moist sawdust-like frass near the whorl and upper leaves of the plant • Indirect effects on grain production Damage on Maize

• Young larvae feed on one side of leaves leaving the epidermis intact on other side • Older larvae feed by making holes in leaves and eat from the edge of the leaves inward • Larval feeding causes extensive defoliation • Larvae can also burrow into the growing point and affect the growth of plants • Larvae sometimes bore into the ear through the husk and feed on kernels Damage on Maize Economic losses

• Outbreaks of Fall Armyworm have been reported in several countries in Africa

• Around 330,000 hectares of staple , especially maize, have been affected

• The remaining African countries remain at high risk.

• The severity of the impact on regional crop production is yet to be established Monitoring

Often done by using blacklight traps and traps

Pheromone traps are very efficient, suspended at canopy height

Insect catches indicate the presence of moths in the area but not good indicators of density

Once the moths are detected, search for eggs and larvae

To assess the proportion of plants infested, random sampling of 20 plants in five locations, or 10 plants in 10 locations, is considered adequate Chemicals in Pheromone lure

• The FAW sex pheromone was reported by Tumlinson et al. (1986) • A mixture of (Z)-9-tetradecen-1-ol acetate, (Z)-9-14:Ac; (Z)-7-dodecen- 1-ol acetate, (Z)-7-12:Ac; (Z)-9-dodecen-1-ol acetate, (Z)-9-12:Ac and (Z)-11-hexadecen-1-ol acetate, (Z)-11-16:Ac • In the ratio of 81: 0.5: 0.5: 18, respectively Companies producing Pheromone traps

• ISCA Technologies, California, U.S.A. • Russell IPM, England • Biocontrole • Pherobank • Scentry monitoring products • NovAgrica • Evergreen growers supply • Biocontrol Research Laboratory, Bangalore, India Control methods

• Physical control • Cultural practices • Host plant resistance • Biological control • Chemical control • IPM • Handpicking egg masses and larvae • Deep plowing to kill pupae in the soil • Placing sand or ash in the whorls

Cultural control

• Intercropping with beans has shown to reduce the FAW infestations by 20‐30 percent. (Needs to be tested) (Needs to be tested) Chemical Control • main control option: > 25 % plants damaged • Spot treatment for isolated areas effective • Late afternoon or early morning- best time • Recommended insecticides: pyrethroids, carbamates and organophosphates • Granular insecticides for young plants (whorl stage) Chemical Control • Limitations -Concealed larvae -Expensive and not affordable -Appropriate safety procedures may not be implemented on regular basis -Personal protective equipment not available -Increased risk of exposure -Resistance development resistance • First noted in 1979, FAW collected from a maize in Tifton, Georgia (U.S.) was shown to be resistant to carbaryl. • In 1991, a strain of FAW collected from maize in northern Florida (U.S.) showed resistance to commonly used insecticides. • Also shown resistance to current pesticides Insecticide resistance management • Treat when needed: follow pressure and thresholds. • Conservation Biological control • Augmentation biological control • Don’t treat successive generations with products of the same mode of action • Use an approximately 30 day window to conduct sprays of insecticides of the same mode of action • Do not apply products of the same mode of action over more than 50% of the crop cycle Insecticides recommended for use

Active ingredient Trade name Abamectin Abba Acetamiprid Gazelle acetamiprid + lambda‐cyhalothrin Bacillus thuringiensis (Bt) var. kurstaki Thuricide Bifenthrin Capture Beauveria bassiana Carbaryl Sevin Chlorantraniliprole Insecticides recommended for use Active ingredient Trade name Chlorantaniliprole +lambda‐cyhalothrin Besiege beta‐cyfluthrin Baythroid gamma‐cyhalothrin Proaxis lambda‐cyhalothrin Warrior II zeta‐cypermethrin Respect Dimethoate Dimethoate Imidacloprid Admire Pro Indoxacarb + acetamiprid Insecticides recommended for use Active ingredient Trade name Metarhizium anisopliae Malathion Malathion Methoxyfenozide Intrepid Permethrin Ambush Spinetoram Delegate Spinosad Success Bayer crop science

• BELT SC 480 – Flubendiamide *** Cancelled By EPA in U.S. • Larvicide – Effective against FAW • Paralyses caterpillars within 1 - 2 hours of application Host plant resistance

• Transgenic/ BT maize varieties • Several varieties on the market that suppress/control FAW and other lepidopteran pests. • CIMMYT is addressing this aspect Biological control

• Classical: involves introducing natural enemies from a pest's native range into a new area where native natural enemies do not provide control.

• Augmentative: involves the massive production and release of natural enemies to control the pest quickly.

• Conservation: involves conservation of existing natural enemies in an environment as these natural enemies are already adapted to the and to the target pest, and their conservation can be simple and cost-effective Egg of Spodoptera spp. Species Order: family Country Agathis stigmatera Hymenoptera: Argentina

Chelonus spp. Hymenoptera: Braconidae Barbados, Nicaragua Honduras, Mexico, Trinidad, Argentina, Brazil Chile, Colombia, Cuba, Haiti, Puerto Rico, U.S., Uruguay, Venezuela Egg parasitoids of Spodoptera spp. Species Order: family Country remus Hymenoptera: Antigua, Barbados, Brazil, Colombia, Platygastridae Dominican Republic, Guadeloupe, Equador, Guyana, Honduras, Nicaragua, Puerto Rico, Suriname, Trinidad, U.S., Venezuela, Israel, India, Telenomus spp. Hymenoptera: Brazil, Colombia, Cuba, Guadeloupe, Platygastridae Mexico, Southeast and South Asia spp. Hymenoptera: Barbados, Nicaragua, Brazil, Chile, Trichogrammatidae Colombia, Argentina, Cuba, U.S., Guadeloupe, Mexico Larval parasitoids of Spodoptera spp. Species Order: family Country Archytas spp. Diptera: Argentina, Barbados, Honduras, Mexico, Nicaragua, U.S., Venezuela, Brazil, Chile, Puerto Rico, Suriname, Trinidad, Uruguay, Cuba, Ecuador, Guadeloupe, Lesser Antilles, Peru spp. Hymenoptera: Braconidae Barbados, Nicaragua Honduras, Mexico, Trinidad, Argentina, Brazil, Chile, Colombia, Cuba, Haiti, Puerto Rico, U.S., Uruguay, Venezuela Hymenoptera: Braconidae Honduras, Barbados, Nicaragua, Argentina, marginiventris Brazil, Chile, Lesser Antilles, Mexico, Puerto Rico, Suriname, U.S., Uruguay, Venezuela, Trinidad & Tobago, Colombia, Guyana Larval parasitoids of Spodoptera spp. Species Order: family Country Ophion spp. Hymenoptera: Argentina, Uruguay, Chile, U.S., Brazil, Honduras, Mexico, Nicargua, Peru, Euplectrus Hymenoptera: Nicaragua, U.S., Argentina, Puerto Rico, spp. Eulophidae Panama, Honduras, Barbados, Brazil, Chile, Colombia, Cuba, Guyana, Lesser Antilles, Mexico, Trinidad, Venezuela, Honduras Lespesia spp. Brazil, Honduras, U.S., Argentina, Brazil, Chile, Cuba, Guadeloupe, Guatemala, Honduras, Lesser Antilles, Mexico, Nicaragua, Puerto Rico, Uruguay, Venezuela, Colombia Pupal parasitoids of Spodoptera spp. Species Order: family Country Brachymeria spp. Hymenoptera: Chalcididae Argentina, U.S. Trichospilus pupivora Hymenoptera: Eulophidae Barbados Cryptus albitarsis Hymenoptera: Ichneumonidae U.S. Diapetimorpha introita Hymenoptera: Ichneumonidae Honduras, U.S. Ichneumon spp. Hymenoptera: Ichneumonidae U.S. Predators

• Doru luteipes () (Dermaptera: Forficulidae) - Used for the biological control of FAW eggs in Brazil - Recommended by Brazil’s national research agency

• Orius insidiosus (pirate bug) (Hemiptera: Anthocoridae) - Commercially produced - Not clear if used for FAW Parasitoids of Spodoptera frugiperda in Florida, U.S.

Species Order: family Telenomus remus Hymenoptera: Platygastridae Aleiodes laphygmae Hymenoptera: Braconidae Meteorus antographae Hymenoptera: Braconidae Cotesia marginiventris Hymenoptera: Braconidae Euplectrus platyhypenae Hymenoptera: Eulophidae Chelonis insularis Hymenoptera:Braconidae Parasitoids of Spodoptera frugiperda in Florida, U.S. Species Order: family Lespesia archippivora Diptera: Tachinidae Lespesia aletiae Diptera: Tachinidae Eucelatoria rubentis Diptera: Tachinidae Ophion flavidus Hymenoptera: Ichneumonidae Parasitoids of Spodoptera frugiperda in Mexico Species Order: family Chelonis insularis Hymenoptera:Braconidae Aleiodes spp. Hymenoptera: Braconidae Glyptapanteles spp. Hymenoptera: Braconidae Cotesia spp. Hymenoptera: Braconidae

Homolobus spp. Hymenoptera: Braconidae Meteorus antographae Hymenoptera: Braconidae Parasitoids of Spodoptera frugiperda in Mexico Species Order: family Campoletis flavicincta Hymenoptera: Ichneumonidae Eiphosoma vitticole Hymenoptera: Ichneumonidae Ophion flavidus Hymenoptera: Ichneumonidae Pristomerus spinator Hymenoptera: Ichneumonidae

Aprostocetus spp. Hymenoptera: Eulophidae

Euplectrus spp. Hymenoptera: Eulophidae

Horismenus spp. Hymenoptera: Eulophidae Parasitoids of Spodoptera frugiperda in Argentina Species Order: family Archytas spp. Diptera: Tachinidae Ophion spp. Hymenoptera: Ichneumonidae Campoletis grioti Hymenoptera: Ichneumonidae

Chelonus insularis Hymenoptera: Braconidae

EuplectrusPlatyhypenae Hymenoptera: Eulophidae

Incamyia chilensis Diptera: Tachinidae Parasitoids of Spodoptera frugiperda in Hondurus Species Order: family Chelonis insularis Hymenoptera: Braconidae Lespesia spp. Diptera: Tachinidae Ophion flavidus Hymenoptera: Ichneumonidae Eiphosoma vitticole Hymenoptera: Ichneumonidae

Archytas spp. Diptera: Tachinidae

Euplectrus spp. Hymenoptera: Eulophidae Parasitoids of Spodoptera frugiperda in Hondurus Species Order: family Pristomerus spinator Hymenoptera: Ichneumonidae Temecula spp. Hymenoptera: Ichneumonidae Microcharops anticarsiae Hymenoptera: Ichneumonidae Cotesia marginiventris Hymenoptera: Ichneumonidae

Rogas spp. Hymenoptera: Braconidae Parasitoids of Spodoptera frugiperda in Brazil Species Order: family Aleiodes laphygmae Hymenoptera: Braconidae Campoletis sonorensis Hymenoptera: Ichneumonidae) Chelonis insularis Hymenoptera: Braconidae Cotesia marginiventris Hymenoptera: Ichneumonidae

Euplectrus plathypenae Hymenoptera: Eulophidae

Homolobus truncator Hymenoptera: Braconidae Parasitoids of Spodoptera frugiperda in Brazil Species Order: family Meteorus laphygmae Hymenoptera: Ichneumonidae Ophion flavidus Hymenoptera: Ichneumonidae

Euplectrus plathypenae Hymenoptera: Eulophidae

Pristomerus spinator Hymenoptera: Ichneumonidae

Trichogramma atopovirilia Hymenoptera: Trichogrammatidae Egg , Telenomus spp. reported in Africa Telenomus spp. Host Distribution T. procas Antigastra catalaunalis , Sudan T. nemesis Chilo orichalcociliellus , Kenya, , Senegal T. busseolae Busseola fusca, Sesamia Cameroon, Egypt, Ghana, Kenya, , spp., Coneista ignefusalis Reunion, Senegal, , Sudan, Uganda T. creusa Chilo diffusilineus Malawi T. bin i Maliarpha separratella, Ghana, Ivory Coast, Madagascar, Malawi, Chilo spp., Scirpophaga Senegal, Tanzania. spp. T. thestor Chilo orichalcociliellus Ivory Coast, Kenya, Senegal, Uganda, Zaire Egg parasitoid, Telenomus spp. reported in Africa

Telenomus spp. Host Distribution T. soudanensis, Chilo zacconius Niger T. nephele Scirpophaga melanoclista, S. Cameroon, Ghana, Ivory Coast, occidentella, S. subumbrosa Malawi, Mali, Senegal. T. etielliphaga Etiella zinckenella, Senegal T. applanatus Eldana saccharina Gabon, Ghana, Ivory coast T. versicolor Scirpophaga melanoclista Ghana, Ivory Coast, Malawi, Senegal Egg parasitoid, Trichogramma spp. reported in Africa

Trichogramma spp. Host Distribution Trichogramma Helicoverpa armigera Morocco bourarachae

Trichogramma bournieri Chilo partellus Comoros, Kenya, Trichogramma cacoeciae - Morocco Trichogramma chilonis Eldana saccharina, Busseola fusca, South Africa Chilo partellus Trichogramma - Cameroon ethiopicum Egg parasitoid, Trichogramma spp. reported in Africa Trichogramma spp. Host Distribution Trichogramma evanescens Chilo Agamemnon, Egypt, Madagascar Helicoverpa armigera, Pectinophora gossypiella, Spodoptera littoralis Trichogramma japonicum Chilo partellus Malawi Trichogramma kalkae Diopsis macrophthalma Malawi Trichogramma sp. nr kalkae - Zimbabwe Trichogramma kayo - Sudan Trichogramma mandelai Diparopsis watersi Chad Egg parasitoid, Trichogramma spp. reported in Africa Trichogramma spp. Host Distribution Trichogramma sp. mwanzai Chilo diffusilineus, Chilo Malawi, Kenya partellus, Busseola fusca, Eldana saccharina, Sitotroga cerealella Trichogramma ostriniae Busseola fusca, Chilo partellus South Africa Trichogramma papilionidis Angola T. Pretiosum leaf roller South Africa Trichogramma pinneyi Diopsis macrophthalma Malawi Egg parasitoid, Trichogramma spp. reported in Africa Trichogramma spp. Host Distribution Trichogrammatoidea eldanae E. saccharina, Sesamia South Africa, Nigeria, calamistis Kenya Trichogrammatoidea lutea C. partellus, B. fusca, H. South Africa, Kenya, armigera, H. armigera Ivory Coast, , Mali, Mozambique, Senegal Trichogrammatoidea simmondsi Diopsis acrophthalma, C. Malawi, South Africa, partellus, False codling Kenya, Burkina Faso , H. armigera, Atherigona soccata Egg parasitoid, Trichogramma spp. reported in Africa Trichogramma spp. Host Distribution Trichogramma sp. mwanzai Chilo diffusilineus, Chilo Malawi, Kenya partellus, Busseola fusca, Eldana saccharina, Sitotroga cerealella Trichogramma ostriniae Busseola fusca, Chilo partellus South Africa Trichogramma papilionidis Angola T. Pretiosum Apple leaf roller South Africa Trichogramma pinneyi Diopsis macrophthalma Malawi Efficacy of egg parasitoids

• The presence of scales/hairs over the egg masses act as a barrier against by Trichogramma spp.

• This difficulty can be overcome by using a more aggressive parasitoid, capable of breaking the physical barrier imposed by scales on the eggs

• It is essential to know the species/strains present in the agro-ecosystem

• Telenomus remus has proved very effective in South America and Florida. Telenomus remus parasitizing spodoptera eggs Trichogramma spp. parasitizing spodoptera eggs Another parsitoid:

H. Hebetor developing on larvae of the MHM Another parsitoid: Habrobracon hebetor

• Wasp, belonging to family braconidae • Used against head miner • Parasitoids cultured in jute bags and dispersed in millet fields Habrobracon hebetor

• In Africa: Algeria, Burkina Faso, Egypt, Libya, Madagascar, Niger, Senegal, South Africa, Zimbabwe, Mauritius (CABI) • Found in Israel • EPPO Countries: Mediterranean region, Portugal, Germany Parasitoids Being reared

• Telenomus remus (Florida, Brazil) • Chelonus sp. (Florida) • Cotesia marginiventris (Florida) • Habrobracon hebetor (Niger, Senegal) Telenomus remus

• Origin – • 1963 – Introduced to India from Papua New Guinea - established • 1971 to 1972 – Introduced to Barbados; later introduced to Antigua, Dominica, Monserrat, St Kitts, St Vincent, Trinidad and Tobago • 1978 – Released in El Salvador and Nicaragua (not established?) • 1975-76 – Released in Florida • 1989 – Introduced to Venezuela – established • 1991-1994 – Intoduced to Honduras Telenomus remus

• 1982 – Introduced to Australia from S.E. Asia and Dominican Republic • 1966-67 – Introduced to Cyprus • 1969 – Introduced to Israel from Papua New Guinea • 1975 – Introduced to New Zealand from Pakistan • 1982 – Indroduced to Pakistan from Trinidad Cotesia marginiventris

• Introduced to Australia in 1982 from U.S.A. • Occurs in Fiji (Maddison, 1982) • Introduced to India in 1969 from U.S.A. • Introduced to Egypt in 1950s from U.S.A. • Found in Central African Republic and Egypt. (CABI) • Used in EPPO countries :Belgium, France, Netherlands, Spain Telenomus nawai • Introduced to Egypt in 1936-37 from Hawaii

Telenomus spodopterae

• Introduced to Egypt in 1940 from Java

Chelonus insularis

• Found in South Africa and Egypt. (CABI) • Other species found in Israel, Egpyt Other biological control options • S. frugiperda nuclear polyhedrosis virus (SFNPV) - Used in Brazil (Cruz et al. 1997, Barreto et al. 2005) - Increased larval mortality; up to 90 percent, Increased parasitoid populations

• Metarhizium anisopliae , Beauveria bassiana - Tested (in vitro) in Mexico, Brazil (Arenas et al. 2014, Thomazoni et al. 2014) - B. bassiana caused mortality 3-90% eggs, 54-100% (Gutierrez et al 1996)

• Seed treatment with Trichoderma induces defense Other biological control options • Botanicals: Anti-feedant, insecticidal effect • Neem based products: Azadirachtin main component - Align -Azatin -Ecozin -Neemazal -Neememusion -Neemix - Ornazin • Tephrosia Summary

• Biocontrol •Biocontrol • Cultural • - Trap crops •Botanical - Push and pull •Host Plant Resistance •Chemical Egg Larva • Physical FAW Adult Pupa

•Physical/ chemical •Biocontrol - Pheromone traps •Physical - Light traps - Ploughing Researchable Areas for Mitigation

• Induce defense in maize plants by treating the seeds with Trichoderma

• Screen pheromone lures from different sources for their efficacy

• Set up pheromone traps for monitoring

• Set up pheromone traps on an area-wide basis for suppression of the population of FAW Researchable Areas for Mitigation • Adoption of trap crops. (castor plants to attract moths to lay eggs; planting young maize plants in mature crops)

• Application of Neem seed extract or commercial neem formulations.

• Survey of FAW locally recruited natural enemies and recording them on a regional and continental basis.

• Multiplication of effective egg/larval parasitoids in the lab for augmentative release. Researchable Areas for Mitigation • Consideration of classical biological control by introducing effective exotic natural enemies, if local natural enemies proven not effective

• Explore push and pull technique

• Use of Bacillus thuringiensis, Beauveria bassiana, Metarhizium anisopliae, and NPV

• Hand-picking caterpillars in small gardens

• Plow the field after harvest to kill pupae in the soil Researchable Areas for Mitigation • Screening insecticides for efficacy

• Use of selected insecticides

• Use of resistant varieties if available

• Use of GMO’s (where approved) IPM: Integrated Pest Management

• IPM developed for an entire cropping system/ crop • Not focused on one pest/ disease • Integration of management practices developed for FAW into the IPM package developed for Maize.