Compromised Fruit: Botrytis Bunch Rot
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PROTOCOL RED WINE Compromised Fruit: Botrytis Bunch Rot VARIETY TYPE OF WINE Incidence of 5% or more of Botrytis cinerea and associated secondary Red Varieties infections. CHALLENGES OBJECTIVE • Oxidation • Off-aromas and flavors • Stuck fermentation • Organic acid depletion Mitigate the detrimental wine matrix alterations from fruit compromised by • Depletion of SO 2 Botrytis bunch rot. • Secondary Infection • Filterability • Biogenic amine • Mycotoxins KEY WINEMAKING STEPS WHEN DEALING WITH BOTRYTIS AFFECTED GRAPES: 1. Hand harvest and sort contaminated grapes in the vineyard. 2. Use adequate antioxidant and antioxidase protection to limit browning, color loss and aroma oxidation. 3. Reduce skin contact to limit extraction of off-aromas; avoid extended maceration and cold soak. 4. Supplement must with amino acids and ammonia to ensure complete healthy fermentation. 5. Press early and select yeast with low nitrogen requirements and fast, complete fermentation kinetics. 6. Promote fast extraction of polyphenols with EnartisZym Color Plus . 7. Remove any spoilage microbes as soon as possible. 8. If there is visual browning, use Enartis Claril SP to remove browning products. 9. Improve color intensity and stability by promoting condensation and co-pigmentation reactions. 10. Adsorb yeast inhibitors and some off-flavors and aromas with Celferm . 11. Balance wine mouthfeel with mannoproteins and fermentation tannins. 12. Late application of certain fungicides can increase elemental sulfur and metal content (Cu); test for metals and use Stabyl Met (PVI/PVP) to fine out excessive heavy metals. The above is achieved to the best of our knowledge and experience. Revision: August 2019 The industrial application of the advice provided does not imply any responsibility on the part of our company. Enartis USA, Inc. 7795 Bell Road | Windsor, CA 95492 | Tel. +1 (707) 838 6312 | Fax + 1 (707) 838 1765 | www.enartis.com PROTOCOL RECOMMENDED VINQUIRY ANALYSIS STAGE ANALYSIS INTERPERTATION Gluconic Acid: • Lower levels of 0.2-0.3 g/L is normal range in wine • 1.0-2.0 g/L indicate Botrytis infection • 2.0-3.0 g/L indicate secondary infections (acetobacter, Juice Botrytis Panel gluconobacter) PCR for Botrytis: • Low risk: < 100 spores/mL • Medium risk: 100-500 spores/mL • High risk: > 500 spores/mL Presence of glucans affect wine filterability and viscosity. Recommend Glucans using EnartisZym Elevage to improve filterability if results are positive. Glycerol Greater than 15 g/L is indicative of infection, mouthfeel effect. After MLF Acetic Acid Volatile Acidity • Normal range in dry table wine: 0.4 g/L • Higher is indicative of spoilage from secondary infections • Filterability Index Before Bottling Filterability Panel • Vmax PROTOCOL WINEMAKING OBJECTIVE ENARTIS RECOMMENDATIONS DOSAGE STAGE Effergran : Effervescent, granulated potassium metabisulfite. Direct addition into gondolas if 125 g/gondolas machine harvested. (5 tons) and Antioxidant, AST : Potassium metabisulfite 50% L-ascorbic acid Antimicrobial 30% Gallic tannin 20%. 10 g/hL (0.8 lb/1000 gal) of Harvest AST provides around 28 mg/L of SO2 and 30 mg/L 100-200 g/ton of ascorbic acid. (At reception) EnartisTan Antibotrytis : Gallic, di-gallic, ellagic and condensed tannins. Antioxidant or 50-200 g/ton EnartisTan Rouge : Micro-granulated blend of gallic, ellagic and condensed tannins. EnartisZym Color Plus : Pectolytic enzyme Enzyme preparation rich in cellulasic and hemicellulasic 20-40 g/ton Grape Reception side activities. EnartisStab Micro M : Pre-activated chitosan from Antimicrobial 80 g/ton Aspergillus niger with purified yeast hulls. The above is achieved to the best of our knowledge and experience. Revision: August 2019 The industrial application of the advice provided does not imply any responsibility on the part of our company. Enartis USA, Inc. 7795 Bell Road | Windsor, CA 95492 | Tel. +1 (707) 838 6312 | Fax + 1 (707) 838 1765 | www.enartis.com PROTOCOL WINEMAKING OBJECTIVE ENARTIS RECOMMENDATIONS DOSAGE STAGE Limit skin contact: no cold soak, no extended maceration Adjust pH and acid at juice stage: pH and Acid Management Panel Maceration EnartisZym Elevage : Pectolytic enzyme preparation with significant ß-glucanase activity. Filterability 5 g/hL Proactive application for improved filterability. Test for glucans post AF. Nutriferm Arom Plus : Autolyzed yeast with an elevated content of free amino acids and survival Yeast Nutrients 40 g/hL factors, thiamine hydrochloride (vitamin B1) (0.15%). EnartisFerm WS Inoculation Yeast EnartisFerm Top 15 20 g/hL (select one) EnartisFerm ES454 EnartisPro Tinto : Blend of Yeast cell walls, grape Polysaccharides seed tannins and ellagic tannins. Improves 20-40 g/hL mouthfeel, promotes color stability and intensity. Nutriferm Advance : DAP, inactivated yeast and Yeast Nutrients 20-40 g/hL cellulose. 1/3 Fermentation Color EnartisTan E : High concentration of mono- Stabilization catechins and low molecular weight condensed 10 g/hL Antioxidant tannins. EnartisML Silver : Oenococcus oeni that ensures ML Malolactic Volume fermentation under difficult conditions due to high Malolactic Bacteria dependent alcohol and polyphenol content. Fermentation Nutriferm ML : Amino acids, vitamins, Nutrients 20-30 g/hL polysaccharides, cellulose and co-factors. Antioxidant Winy : Potassium metabisulfite. 0.5 ppm Antimicrobial Molecular EnartisStab Micro : Pre-activated chitosan from Maturation Antimicrobial 10 g/hL Aspergillus niger. EnartisZym Elevage : Pectolytic enzyme Filterability 5 g/hL preparation with significant ß-glucanase activity. Mouthfeel Enartis maturation and finishing tannins, Surli Velvet Finishing Trial Dependent Structure (soluble yeast mannoproteins) For more information about how to treat Botrytis infected grapes and wine, please contact Enartis Wine Services at (707) 838-6312 ext. 4. The above is achieved to the best of our knowledge and experience. Revision: August 2019 The industrial application of the advice provided does not imply any responsibility on the part of our company. Enartis USA, Inc. 7795 Bell Road | Windsor, CA 95492 | Tel. +1 (707) 838 6312 | Fax + 1 (707) 838 1765 | www.enartis.com PROTOCOL CITATIONS: Cinquanta, L., Albanese, D., De Curtis, F., Malvano, F., Crescitelli, A., & Di Matteo, M. (2015). Rapid assessment of gray mold (Botrytis cinerea) infection in grapes with a biosensor system. American Journal of Enology and Viticulture , 66 (4), 502-508. Couto, J. A., Graça, A. R., Soares-Franco, J. M., & Hogg, T. (2003, June). The use of gluconic acid level as an indicator of the activity of acetic acid bacteria in grapes. In VII Symposium International d’Oenologie, Bordeaux, France . Lopez Pinar, A., Rauhut, D., Ruehl, E., & Buettner, A. (2017). Effects of bunch rot (Botrytis cinerea) and powdery mildew (Erysiphe necator) fungal diseases on wine aroma. Frontiers in chemistry , 5, 20. Steel, C. C., Blackman, J. W., & Schmidtke, L. M. (2013). Grapevine bunch rots: impacts on wine composition, quality, and potential procedures for the removal of wine faults. Journal of agricultural and food chemistry , 61 (22), 5189-5206. Vignault, A., Pascual, O., Jourdes, M., Moine, V., Fermaud, M., Roudet, J., & Zamora, F. (2019). Impact of enological tannins on laccase activity. OENO One , 53 (1). The above is achieved to the best of our knowledge and experience. Revision: August 2019 The industrial application of the advice provided does not imply any responsibility on the part of our company. Enartis USA, Inc. 7795 Bell Road | Windsor, CA 95492 | Tel. +1 (707) 838 6312 | Fax + 1 (707) 838 1765 | www.enartis.com .