PODXL Antibody

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Efficient Professional Protein and Antibody Platforms PODXL Antibody Basic information: Catalog No.: UMA60669 Source: Mouse Size: 50ul/100ul Clonality: Monoclonal Concentration: 1mg/ml Isotype: Mouse IgG1 Purification: Protein G affinity purified Useful Information: Applications: ICC:1:200-1:500 Reactivity: Human Specificity: This antibody recognizes PODXL protein. Immunogen: recombinant protein Podocalyxin, a sialoglycoprotein, is thought to be the major constituent of the glycocalyx of podocytes. It is a member of the CD34 family of trans- membrane sialomucins. It is involved in the regulation of both adhesion and cell morphology and cancer progression. It functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Podocalyxin acts as a Description: pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integ- rin-dependent manner. Sialylated, O-glycosylated glycoforms of podocalyxin expressed by colon carcinoma cells possess L-selectin and E-selectin binding activity, and may be pivotal to the metastatic spread of colon carcinoma cells. Uniprot: O00592 BiowMW: 58kDa Buffer: 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data: ICC staining PODXL in HCT116 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] .

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Podocalyxin Is Required for Maintaining Blood–Brain Barrier Function During Acute Inflammation
Podocalyxin is required for maintaining blood–brain barrier function during acute inflammation Jessica Caita, Michael R. Hughesa,1, Matthew R. Zeglinskib,c,d, Allen W. Chane,f,g,h,i, Sabrina Osterhofa, R. Wilder Scotta,j, Diana Canals Hernaeza, Alissa Caita, A. Wayne Voglj,k, Pascal Bernatchezd,l, T. Michael Underhilla,j, David J. Granvilleb,c,d, Timothy H. Murphye,f,g, Calvin D. Roskelleyj,k, and Kelly M. McNagnya,1 aBiomedical Research Centre, University of British Columbia, Vancouver, BC, Canada V6T 1Z3; bICORD Centre, University of British Columbia, Vancouver, BC, Canada V5Z 1M9; cDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada V6T 2B5; dCentre for Heart and Lung Innovation, St Paul’s Hospital, Vancouver, BC, Canada V6Z 1Y6; eDepartment of Psychiatry, University of British Columbia, Vancouver, BC, Canada V6T 2A1; fKinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, BC, Canada V6T 1Z4; gDjavad Mowafaghian Center for Brain Health, University of British Columbia, Vancouver, BC, Canada V6T 1Z3; hNeuroscience and Mental Health Institute, University of Alberta, Edmonton, AB, Canada T6G 2R3; iNeurochemical Research Unit, Department of Psychiatry, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada T6G 2R3; jDepartment of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada V6T 1Z4; kLife Sciences Institute, University of British Columbia, Vancouver, BC, Canada V6T 1Z4; and lDepartment of Anaesthesiology, Pharmacology & Therapeutics, University of British Columbia, Vancouver, BC, Canada V6T 1Z3 Edited by Lawrence Steinman, Stanford University School of Medicine, Stanford, CA, and approved January 15, 2019 (received for review September 6, 2018) Podocalyxin (Podxl) is broadly expressed on the luminal face of and plasma factors to effect repair.
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Exome Sequencing and in Vitro Studies Identified Podocalyxin As a Candidate Gene for Focal and Segmental Glomerulosclerosis
Exome sequencing and in vitro studies identified podocalyxin as a candidate gene for focal and segmental glomerulosclerosis The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Barua, Moumita, Eric Shieh, Johannes Schlondorff, Giulio Genovese, Bernard S Kaplan, and Martin R Pollak. 2013. “Exome sequencing and in vitro studies identified podocalyxin as a candidate gene for focal and segmental glomerulosclerosis.” Kidney international 85 (1): 124-133. doi:10.1038/ki.2013.354. http://dx.doi.org/10.1038/ ki.2013.354. Published Version doi:10.1038/ki.2013.354 Citable link http://nrs.harvard.edu/urn-3:HUL.InstRepos:12717580 Terms of Use This article was downloaded from Harvard University’s DASH repository, and is made available under the terms and conditions applicable to Other Posted Material, as set forth at http:// nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of- use#LAA NIH Public Access Author Manuscript Kidney Int. Author manuscript; available in PMC 2014 July 01. NIH-PA Author ManuscriptPublished NIH-PA Author Manuscript in final edited NIH-PA Author Manuscript form as: Kidney Int. 2014 January ; 85(1): 124–133. doi:10.1038/ki.2013.354. Exome sequencing and in vitro studies identified podocalyxin as a candidate gene for focal and segmental glomerulosclerosis Moumita Barua1,2,+, Eric Shieh2,+, Johannes Schlondorff1,2, Giulio Genovese3,4,5, Bernard S Kaplan6, and Martin R Pollak1,2 1Division of Nephrology, Dept. of Medicine, Beth Israel Deaconess
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Anti-PODXL Antibody (ARG41386)
Product datasheet [email protected] ARG41386 Package: 100 μl anti-PODXL antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes PODXL Tested Reactivity Hu Tested Application FACS, ICC/IF, IHC-P, WB Host Rabbit Clonality Polyclonal Isotype IgG Target Name PODXL Antigen Species Human Immunogen Synthetic peptide derived from Human PODXL. Conjugation Un-conjugated Alternate Names Gp200; GCTM-2 antigen; PCLP; Podocalyxin; PC; PCLP-1; Podocalyxin-like protein 1 Application Instructions Application table Application Dilution FACS 1:100 ICC/IF 1:100 - 1:500 IHC-P 1:50 - 1:200 WB 1:500 - 1:2000 Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Calculated Mw 59 kDa Properties Form Liquid Purification Affinity purified. Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. Preservative 0.02% Sodium azide Stabilizer 50% Glycerol Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. www.arigobio.com 1/2 Note For laboratory research only, not for drug, diagnostic or other use. Bioinformation Gene Symbol PODXL Gene Full Name podocalyxin-like Background This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane.
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Inline-Supplementary-Material-6.Pdf
Table S3. Transcriptome analysis page 1 Table S3. Transcriptomic analysis of cultured mutant and wildtype skin fibroblast. Gene Protein name GeneCards annotation OMIM Locus Fold FDR PATIENTS CONTROLS - change (n=2) (n=4) ment apoptosis apoptosis Keratinocyte Keratinocyte inflammation differentiation Neurodevelop cell growth and growth cell FPKM [SD] FPKM [SD] genes up-regulated in patient cells ESRP1 epithelial ESRP1 is an mRNA splicing factor that regulates the *612959, the gene product is an 8:95653301 - present - 0.011 0.3547 0.0038 0.0000 0.0000 splicing formation of epithelial cell-specific isoforms. epithelial cell-type-specific splicing 95719694 absent regulatory Specifically regulates the expression of FGFR2-IIIb, regulator. Mutations in ESRP1 are protein 1 an epithelial cell-specific isoform of FGFR2. Also potentially associated with autosomal regulates the splicing of CD44, CTNND1, ENAH, recessive deafness type 109. three transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). CERS3 ceramide CERS3 is a member of the ceramide synthase family #615023, autosomal recessive 15:100913143 - present - 0.011 0.1844 0.0118 0.0000 0.0000 synthase 3 of genes. The ceramide synthase enzymes regulate congenital ichthyosis type 9, 101085200 absent sphingolipid synthesis by catalyzing the formation characterized by collodian membrane at of ceramides from sphingoid base and acyl-CoA birth, acanthosis, orthohyperkeratosis, substrates. This family member is involved in the fine erythrodermic scales, palmoplantar synthesis of ceramides with ultra-long-chain acyl- hyperlinearity. moieties (ULC-Cers), important to the epidermis in its role in creating a protective barrier from the environment. GJB6 gap junction GJB6 encodes one of the connexin proteins.
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