Published Research Articles in International Journals 2014-2015
Suez Canal University Post-Graduate Studies &Research Sector
Published Research Articles in International Journals
Suez Canal University (Abstracts)
2014, 2015
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Published Research Articles in International Journals 2014-2015
جامعة قناة السويس
قطاع الدراسات العليا والبحوث
ملخص االبحـاث العلميـــة المنشــورة بالدوريات العلمية العالمــية
جامعة قناة السويس
2015-2014
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Published Research Articles in International Journals 2014-2015
كلمة السيد االستاذ الدكتور رئيس جامعة قناة السويس
يعد البحث العلمى أداة األمم للتقدم وصناعة الحضارة واالرتقاء بالشعوب وتحقيق رفاهيتها ، ويعد ما تمتلكه أى أمة من أبحاث علمية متقدمة وما تمتلكه من تراث علمى دقيق أحد المعايير المهمة للحكم على تقدم األمة ، ولذا يشهد العالم سباقا وتعاونا فى هذا المجال حتى يستطيع االنسان تسخير قوى الطبيعة وثرواتها لراحته وسعادته .
كما يعد البحث العلمى الدعامة االساسية لالقتصاد والتطور وقناة مهمة الثراء المعرفة االنسانية فى ميادينها كافة ، لذا فإن ما تمتلكه األمة من علماء يعتبر ثروة تفوق كل الثروات الطبيعية .
ولذلك تحرص جامعة قناة السويس على تشجيع النشر الدولى الذى سيضع الجامعة فى موقع ألئق ضمن التصنيف العالمى للجامعات ، والذى يعتمد من بين معاييره على عدد االبحاث العلمية المنشورة بالدوريات العلمية العالمية ، وتنتهج الجامعة طريقا لتنمية االبداع والتفكير العلمى لدى الشباب حتى يمكن تحقيق التقدم وبناء مستقبل مشرق .
وفقنا هللا لما فيه الخير لمصرنا الحبيبة أ.د/ ممدوح مصطفى غراب رئيس جامعة قناة السويس
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Published Research Articles in International Journals 2014-2015
أصبح البحث العلمى واحد من المجاالت الهامة التى تجعل الدول تتطور بسرعة هائلة وتتغلب على المشكالت التى تواجهها بطرق علمية حيث ان البحث العلمى فى حياة االنسان ينبع من مصدرين هامين وهما : المصدر االول:- يتمثل فى االنتفاع بفوائد تطبيقية حيث تقوم الجهات المسئولة بتطبيق هذه الفوائد التى نجمت عن االبحاث. المصدر الثانى:- يتمثل فى االسلوب العلمى فى البحث الذى يبنى عليه جميع المكتشفات واالختراعات .
ويعتبر النشر الدولى منتج اساسى للبحث العلمى الجيد لما له من اهمية كبيرة لدى الجامعات والمراكز البحثية على مستوى العالم، والنشر الدولى هو نشر نتائج االبحاث العلمية فى الدوريات العلمية العالمية المحكمة من قبل اساتذة متخصصين فى فروع العلوم واالّداب المختلفة بينما المدلول الفعلى واالكثر اهمية للنشر الدولى من وجه نظر الباحثين والعلماء على مستوى العالم هو وصول نتائج االبحاث لكافة المتخصصين والباحثين والعلماء فى ذلك الفرع من العلم .
كما اصبح تمويل المشروعات البحثية فى معظم انحاء العالم يعتمد ايضا على عدد االبحاث الدولية المنشورة للباحثين والمجموعات البحثية ولذلك اتجهت الجامعات المصرية فى االّونة االخيرة الى تحفيز باحثيها وعلمائها للنشر الدولى بمنح جوائز مالية تعتمد على تصنيف الدوريات العلمية ومعامل تأثير Impact Factor لكل مجلة مما ادى الى زيادة واضحة فى معدل النشر الدولى للباحثين بالجامعات المصرية ، كذلك ومنذ عام 2009 اصبح النشر الدولى فى الدوريات العلمية المحكمة من اهم عوامل تقييم المتقدمين لجوائز الدولة المختلفة ) تشجيعية – تفوق – تقديرية ( .
ونظرا لموقع جامعة قناة السويس الذى يعتبر الركيزة العلمية االساسية فى المنطقة والتى تعتبر من اهم المناطق التنموية فى الوقت الحالى ونظرا للخبرات المتعددة للسادة االساتذة اعضاء هيئة التدريس بالجامعة فى الكثير من المجاالت لذا تشجع الجامعة وخاصة قطاع الدراسات العليا والبحوث السادة الباحثين من الشباب على النشر بالطريقة السليمة وذلك من خالل عمل دورات متخصصة فى الموضوعات االتية : . طرق الكتابة العلمية المتخصصة . كيفية النشر العلمى بالطريقة الصحيحة . كيفية كتابة المشروعات . اخالقيات البحث العلمى
ولذا فان الهدف االساسى للجامعة هو بناء مجتمع مبتكر يمتاز بالقدرات العلمية والتكنولوجية وتوجيه شباب الباحثين الى التطور واالبتكار لتحقيق اعظم النتائج .
ا.د/ ناهد محمد مصطفى على نائب رئيس الجامعة للدراسات العليا والبحوث
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Published Research Articles in International Journals 2014-2015
A Word by Suez Canal University President
Scientific Research is considered the tool for achieving progress, making civilization and elevating nations. For that reason, what each nation what each nation gains from advanced scientific research and scientific legacy is one of the important criterions to judge its progress. That is why the world today is witnessing a scientific race, as well as scientific cooperation, so that humans can employ the forces and wealth of nature for both comfort and happiness.
Scientific research is also considered the main pillar for economy and development and an important channel to enrich human knowledge in all its fields.
That is why the Suez Canal University is keen to encourage international publication to have a prestigious position at the international ranking of universities, based on the number of scientific Publications in international peer-reviewed journals, books and conferences, Suez Canal University is well on its way to promote creativity and scientific thinking in the youth, so they can achieve progress and build a bright future.
May Allah guide us to the welfare of our beloved Egypt
Prof.MamdouhmostafaGhourab Suez Canal University President Pag 5
Published Research Articles in International Journals 2014-2015
The scientific research has become one of the main fields that make the countries develop rapidly and scientifically overcome problems that face them. The scientific research in human life comes from two main resources: - First resource: - is to benefit from the applied outcomes, and the responsible agencies to apply those resulted benefits. Second resource: - is the scientific approach in research, upon which all discoveries and inventions are depending. And the international publication is considered a main production of the good scientific research. Also for the great importance it holds at universities and the research centers all around the world. The international publication is to publish the scientific research in international scientific periodicals, which is being judged by special professors in different science and arts fields. But the main contribution for the international publication, from the scientists and researchers point of view, is to reach all the results of the latest researches for all the specialists, scientists, and researchers all around the globe. Also the scientific projects finance, everywhere around the world, is depending on the number of published scientific researches for researchers and research groups. And so, recently, the Egyptian universities started to encourage their own researchers and scientists to internationally publish by giving financial awards depending on the periodicals rating and the Impact Factor for each one, which lead to a great increase to the international publication rate for the Egyptian universities researchers. Also since the year 2009, the international publication in judged scientific periodicals has become one of the main qualifications for candidates for different state awards (encouragement – excellence - discretionary). And for the important position of the Suez Canal university, which is the main educational pillar for a whole region being considered one of the most important development regions in the present, and for the different experiences of the faculty‟s professors in all different fields, the university, and especially the researches and graduate studies sector, encourages the youth researchers to publish in the right way. And that will be through holding specialized courses in the following subjects: - - specialized scientific writing methods - how to correctly publish a scientific research - how to write projects - scientific research ethics And so the main goal for the university is to build an innovative society with scientific and technological capabilities and to direct young researchers to develop and innovate to achieve the greatest results.
Prof.Nahed Mohamed Mostafa Ali Vice-President for Postgraduate Studies and Research Suez Canal University Pag 6
Published Research Articles in International Journals 2014-2015
List of Content
NAME OF FACULTY NO.
8-17 Faculty of Medicine
Faculty of Vet. Medicine 18-36
Faculty of Pharmacy 37-50
Faculty of Agriculture 51-66
Faculty of Computing and Informatics 67-71
Faculty of Engineering – Ismailia 72-74
Faculty of Science 75-110
Faculty of Science – El Arish 111-114
Faculty of Agriculture Science – El Arish 115-118
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Therapeutics and Clinical Risk Management 2015 )11( 279–288
Effect of vitamin D therapy on interleukin-6, visfatin, and hyaluronic acid levels in chronic hepatitis C Egyptian patients.
Dina Sabry1 Mohamed ASAl-Ghussein2 Gehan Hamdy3 Amr Abul-Fotouh4 Tarek Motawi5 Amany Y El Kazaz6 Ahmed Eldemery7 Marwa Shaker8, 9
6Medical Biochemistry Department, Faculty of Medicine, Suez Canal University, Ismaileya, Egypt.
Abstract
Objectives: We aimed to compare serum levels of interleukin-6, visfatin, and hyaluronic acid in chronic hepatitis C Egyptian patients who received standard of care (SOC) therapy for chronic hepatitis C virus (HCV) consisting of pegylated interferon (PEG-IFN) and ribavirin (RBV) and in those who received SOC with vitamin D (vit D) for 48 weeks in HCV genotype 4a subjects. Design and methods: One hundred chronic HCV patients were classified into two groups: study 50 patients received SOC therapy PEG-IFN/RBV + vit D and control 50 patients received SOC PEG-IFN/RBV without vit D. Both groups were followed up at 12 weeks, 24 weeks, and 48 weeks of treatment. Results: Results showed a significant elevation in vit D levels in the group treated with SOC and vit D compared to SOC group and a reduction in HCV RNA from the 12th week to reach zero level in the 24th week. Interleukin-6, visfatin, and hyaluronic acid levels were also reduced significantly. Alanine transaminase and aspartate transaminase biomarkers were significantly reduced, indicating decreased liver injury. Conclusion: SOC PEG-IFN/RBV + vit D therapy for chronic HCV led to reduced interleukin- 6, visfatin, and hyaluronic acid levels and follow up liver biochemical biomarkers as aspartate transaminase and alanine transaminase indicates proper liver healing and monitoring. Keywords: hepatitis C virus, HCV 4a RNA, pegylated interferon, ribavirin, AST, ALT
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Published Research Articles in International Journals 2014-2015
Molecular Diagnosis & Therapy 2015 19:365–373
MicroRNA-21 expression in primary breast cancer tissue among female patients and its correlation with chromosome 17 aneusomy
1 1 2 1 Noura R. Abdel-hamid , Eman A. Mohammed , Ashraf H. Abbas , Fouad M. Badr
1 Genetics Unit, Department of Histology and Cell Biology, 2Department of Plastic Surgery, Faculty Of Medicine, Suez Canal University, Ismailia, Egypt.
Abstract
Breast cancer (BC) is the most common cancer worldwide and amongst Egyptian women. Micro-RNA-21 (miR-21), on chromosome 17q21.3, is one of the most up-regulated microRNAs in cancer that silences multiple target genes involved in cancer-signaling pathways. The study assessed the correlation between the miR-21expression profile and numerical aberrations of chromosome 17 in BC tissues in female Egyptian patients. The study included 37 female patients with sporadic primary breast carcinoma, their age ranged from31 to 65 years. Fresh breast tissue specimens were evaluated for miR-21 expression levels using reverse transcription-polymerase chain reaction technology and cytogenetic fluorescent in situ hybridization analysis for chromosome 17 aneusomy. miR- 21 was up-regulated 12.9-fold in BC tissues and over-expression was significantly associated with several clinico-pathologic characteristics; as tumor size, tumor grade, advanced stage, and poor prognostic index. In addition, chromosome 17 aneusomy was, profoundly, observed in BC patients. However, the large majority (73 %) of patients had heterogeneous cell populations. Chromosome 17 copy number heterogeneity in cell populations were significantly associated with advanced clinical stage and higher miR-21 expression profile in BC tissues. In conclusion, in breast cancer, expression of microRNA-21, located on 17q21.3, was correlated with the chromosome copy number. Chromosome 17 aneusomy and microRNA- 21 levels can serve as potential prognostic biomarkers in breast cancer tissues. Chromosome 17 aneusomy analysis by cell heterogeneity gives more useful information than analysis by copy number variation alone.
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Published Research Articles in International Journals 2014-2015
Molecular Diagnosis & Therapy 2015 19:179–190
Pilot study of Serum MicroRNA-21 as a Diagnostic and Prognostic Biomarker in Egyptian Breast Cancer Patients
Eman A. Toraih1, Eman A. Mohammed1, Sherif Farrag2, Nevene Ramsis3, Somaya Hosny4.
1 Genetics Unit, Department of Histology and Cell Biology, 2Department of Surgical Oncology, 3 Department of Clinical Pathology, 4 Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Abstract Background: MicroRNAs are small RNA molecules that bind to complementary sequence of target messenger RNAs and down-regulate their translation to protein or degrade them. MicroRNAs play critical roles in many different cellular processes. Hence, aberrant microRNA expression is common in a variety of disorders, including cancer. Patients and Methods: In this work, we quantified serum microRNA-21 (miR-21) expression levels in 30 breast cancer patients, 30 cancer-free individuals with risk factors for developing breast cancer, and another 30 controls without risk factors, in order to test its role as a possible diagnostic and prognostic biomarker in breast cancer. Results: Our results indicated that miR-21 expression was remarkably elevated in asymptomatic high-risk individuals (2.98-fold) compared to healthy non-risky controls (p<0.001), and was highly increased in almost all sera of cancer patients (12.72-fold) compared to healthy controls (p<0.001). Higher levels of serum miR-21 were also correlated with tumors of higher grades, more nodal involvement, distal metastasis and advanced clinical stages (p<0.01). Furthermore, over-expression levels declined towards normal after surgical tumor resection (p<0.001). Conclusion: In conclusion, our findings demonstrate that serum miR-21 expression profile may serve as a potential non-invasive diagnostic and prognostic biomarker for BC.
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Published Research Articles in International Journals 2014-2015
International Immunopharmacology 2015 24: 80-87
Combination of Coenzyme Q10 with methotrexate suppresses Freund's Complete Adjuvant -induced synovial inflammation with reduced hepatotoxicity in rats: effect on oxidative stress and inflammation
Mona K. Tawfik*
Departments of Pharmacology*, Faculty of Medicine, Suez Canal University
Abstract
Methotrexate (MTX) is a cornerstone disease modifying anti-rheumatic drug. One of its major drawbacks is hepatotoxicity, resulting in poor compliance of therapy. Coenzyme Q10 (CoQ10) is an antioxidant and anti-inflammatory compound, possessing both anti- arthritic and hepatoprotective potential. The present study was carried out to evaluate the effect of CoQ10 (10 mg/kg) alone and in combination with MTX (2 mg/kg) on the progression of adjuvant-induced arthritis in rats, and to elucidate the potential properties of CoQ10 in ameliorating MTX-induced liver damage in rats. Rats were assigned to; normal, arthritic, MTX treated, CoQ10 treated or a combination of MTX and CoQ10. CoQ10 administration potentiated the antiarthritic effect of MTX. Moreover, the combination therapy was effective in attenuating the severity of MTX-induced liver damage displayed by the improvement in hepatospecific serum markers and confirmed by the histo- pathological evaluation. Additionally, it attenuated the hepatic oxidative stress and the intensity of inflammatory mediators associated with MTX administration as evident by the regulation of oxidant/anti-oxidant balance and the inhibitory effects on TNF-α and IL-6 levels. These results revealed that CoQ10 can serve as a useful adjuvant and promote the safe use of MTX in the management of arthritis, not only by potentiating the antiarthritic effect of MTX, but also by alleviating MTX-induced hepatocellular injury.
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Published Research Articles in International Journals 2014-2015
BMJ Open Diabetes Research and Care 2015 3:e000111
Impact of an individualized type 2diabetes education program on clinicaloutcomes during Ramadan. Laura N McEwen,1 Mahmoud Ibrahim,2 Nahed M Ali,3 Samir H Assaad-Khalil,4HyamRefaat Tantawi,5 Gamela Nasr,3Shayan Mohammadmoradi,6 Aly A Misha‟l,7Firas A Annabi,7 Ebtesam M Ba-Essa,8 Suhad M Bahijri,9 Jaakko Tuomilehto,10Linda A Jaber,11 William H Herman,1 3 School of Medicine, Suez Canal University, Ismailia, Egypt
Abstract
Objective: To determine if individualized educationbefore Ramadan results in a safer fast for people withtype 2 diabetes. Methods: Patients with type 2 diabetes who receivedcare from participating clinics in Egypt, Iran, Jordanand Saudi Arabia and intended to fast during Ramadan2014 were prospectively studied. Twelve clinicsparticipated. Individualized education addressed mealplanning, physical activity, blood glucose monitoringand acute metabolic complications and when deemednecessary, provided an individualized diabetestreatment plan. Results: 774 people met study criteria, 515 receivedindividualized education and 259 received usual care.Those who received individualized education weremore likely to modify their diabetes treatment planduring Ramadan (97% vs 88%, p<0.0001), to perform self-monitoring of blood glucose at least twice dailyduring Ramadan (70% vs 51%, p<0.0001), and tohave improved knowledge about hypoglycemic signsand symptoms (p=0.0007). Those who receivedindividualized education also reduced their body mass index (−1.1±2.4 kg/m2 vs−0.2±1.7 kg/m2, p<0.0001)and glycatedhaemoglobin (−0.7±1.1% vs−0.1±1.3%,p<0.0001) during Ramadan compared those whoreceived usual care. There were more mild (77% vs67%, p=0.0031) and moderate (38% vs 19%,p<0.0001) hypoglycemic events reported byparticipants who received individualized education than those who received usual care, but fewer reportedsevere hypoglycemic events during Ramadan (23% vs34%, p=0.0017). Conclusions: This individualized education anddiabetes treatment program helped patients with type 2diabetes lose weight, improve glycemic control andachieve a safer fast during Ramadan.
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Published Research Articles in International Journals 2014-2015
The Journal of Laryngology & Otology JLO 2015 (11):1069-72
Outcome evaluation of clarithromycin,metronidazole and lansoprazole regimens in Helicobacter pylori positive or negative children with resistant otitis media with effusion.
D MEL-HENNAWI, M R AHMED Otolaryngology Unit, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
Abstract
Objective: To compare the efficacy of two treatment regimens among Helicobacter pylori stool antigen positive children suffering from resistant otitis media with effusion.
Methods: The study comprised 258 children with bilateral otitis media with effusion; 134 were positive for H pylori stool antigen, and were equally and randomly allocated to the control group or study group. Thecontrol group received standard otitis media with effusion therapy (amoxicillin and clavulanate), while the study group received standard H pylori triple therapy (clarithromycin, metronidazole and lansoprazole).
Results: In the control group, there was a marked clinical response to treatment in 33 of the 67 children (49.3 per cent). In the study group, there was a marked response in a significantly higher number of children (46 out of 67, 68.7 per cent). The 124 H pylori stool antigen negative children not included in the 2 aforementioned groups received amoxicillin and clavulanate, and a marked response in symptoms was evident in 98 of these children (79 per cent).
Conclusion: H pylori infection may lead to resistance to traditional otitis media with effusion treatment in some cases. H pylori eradication is associated with a high cure rate.
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Published Research Articles in International Journals 2014-2015
Knee Surgery Sports Traumatology Arthroscopy 2015 (2):386-392
Biomechanical evaluation of suture–tendon interface and tissue holding of three suture configurations in torn and degenerated versus intact human rotator cuffs. Ashraf Abdelkafy
Orthopaedic Surgery and Traumatology Department, Faculty of Medicine, Suez Canal University, Circular Road, Ismailia 41522, Egypt.
Abstract
Purpose: The purpose of this study was to biomechanically evaluate suture–tendon interface and tissue holding of three sutures in torn and degenerated versus intact human rotator cuffs. Methods: Sixty-three human rotator cuff tendons were divided into torn degenerated group (TDG), n=21 and intact group (IG), n=42. Ultimate tension load (UTL) and cyclic loading were tested for three arthroscopic sutures: simple, horizontal, and massive cuff sutures (MCS). Results: Ultimate tension load was significantly higher(p\0.05) for the MCS (194±68 N) in comparison with the simple (105±48 N) and horizontal sutures (141 ±49 N) in IG. In TDG, UTL was not significantly higher (n.s.) for MCS (118±49 N), simple (79±30 N), and horizontal sutures (107±28 N) in comparison with IG. MCS (118±49 N) showed no significantly superiorUTL in comparison with the simple and horizontal sutures in the TDG. MCA elongation was 0.6 ±0.7 mm in the IG and 1.3±0.7 mm in the TDG, while horizontal suture elongation was 0.7±0.4 mm in the IG and 1.3±0.5 mm in the TDG. Simple suture elongation was 1.1 ±0.5 mm in the IG and 1.6 ±0.7 mm in the TDG. Conclusion: Human torn and degenerated rotator cuffs have poor tissue quality, significantly lower UTL andhigher cyclic elongation in comparison with intact cuffs regardless of the type of suture used for repair, which invites the need for repair techniques that grasps greater tissue volume in addition to augmentation techniques. Clinical relevance: Clinicians better use repair techniques that grasp greater tissue volume (e.g. MCS, modified Mason–Allen cross bridge, double-row cross bridge, etc.) when repairing the torn and degenerated rotator cuffs. Keywords: Human rotator cuffs, Biomechanical testing, Ultimate tension load, Cyclic loading, Rotator cuff tears, Augmentation techniques.
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The Journal of Laryngology & Otology 2015 129, 337–341
Vestibular dysfunction in patients with post-mumps sensorineural hearing loss
M M EL-BADRY1 , A ABOUSETTA2 , R M A KADER1 1 Audiology Unit, Otolaryngology Department, Minia University, and 2 Audiology Unit, Otolaryngology Department, Suez Canal University, Egypt.
Abstract
Objective: To study the possible damage to the vestibular system in patients with post- mumps sensorineural hearing loss. Methods: Nineteen patients with recent mumps infection participated in the study. All patients had unilateral profound sensorineural hearing loss or total hearing loss. Patients were subjected to video-nystagmography and vestibular-evoked myogenic potential testing. Results: Eight patients (42.1 per cent) had normal video-nystagmography results and intact vestibular-evoked myogenic potentials on both sides, whereas the other 11 patients (57.9 per cent) had vestibular lesions in the form of marked canal weakness and absent vestibular-evoked myogenic potential responses on the same side as hearing loss. The overall findings indicated a peripheral site for the lesions. Conclusion: The majority of patients with post-mumps sensorineural hearing loss had peripheral vestibular pathology in the same ear as hearing loss. Further research should be directed to saving the inner ear following mumps infection.
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Published Research Articles in International Journals 2014-2015
Pharmacological reports 2015 )67(102-114
Myelosuppressive and hepatotoxic potential of leflunomide and methotrexate combination in a rat model of rheumatoid arthritis
Shymaa E. Bilasya, Soha S. Essawyb, Mohamed F. Mandourc, Eman A.I. Alid, Sawsan A. Zaitone A Department of Biochemistry, Fuclty of Pharmacy, Suez Canal University, Ismailia, Egypt. b Department of Pharmacology, Fuclty of Medicine, Suez Canal University, Ismailia, Egypt. c Department of Clinical pathology, Fuclty of Medicine, Suez Canal University, Ismailia, Egypt. d Department of Histology, Fuclty of Medicine, Suez Canal University, Ismailia, Egypt. e Department of Pharmacology & Toxicology, Fuclty of Pharmacy, Suez Canal University, Ismailia, Egypt.
Abstract
Background: Safety of the combination of leflunomide and methotrexate was examined in several studies with inconclusive results. The present study was designed to compare the efficacy and safety of the combination of leflunomide and methotrexate in adjuvant-induced arthritis (AIA) in rats focusing on immunosuppressive and hepatotoxic effects. Methods: Eighty four rats were divided into seven groups. Group 1: Sham control, group 2: the vehicle control, group 3: methotrexate group, group 4-5: leflunomide (5 & 10 mg/kg/day) groups, group 6-7: combination 1&2 [methotrexate + leflunomide (5 & 10 mg/kg/day)] groups, respectively. Results: The current results indicated that combination therapies improved the ankle circumference and clinical scores compared to monotherapies; histopathological examination confirmed these findings. The myelosuppressive effect of leflunomide (10 mg/kg/day) was comparable to that produced by methotrexate as indicated by the complete blood count and bone marrow cellularity; however their combinationresulted in greater toxicity. Furthermore, methotrexate greatly affected the splenic histopathology compared to leflunomide and the combination therapy produced a greater effect compared to leflunomide not methotrexate. Differently, assessment of the hepatotoxic potential of the two drugs highlighted that leflunomide induced a dose-dependent increase in the fibrosis score which was higher in their magnitude than that induced by methotrexate. Leflunomide (10 mg/kg/day) and combination 2 groups showed the greatest degree of liver fibrosis. Conclusions: In rats with AIA, current drug combinations provided higher therapeutic benefit compared to monotherapies, however, greater toxicities were observed. Therefore, continuous monitoring of hematologic parameters and liver function will be recommended in clinical settings.
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Journal of Reproduction and Development 2015 )61(54-60
Persistence of uterine bacterial infection, and its associations with endometritis and ovarian function in postpartum dairy cows
Mohamed ElshabrawyGhanema,b, ErisaTezukaa, BhuminandDevkotaa,Yoshiaki Izaikeaand Takeshi Osawaa aDepartment of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan bDepartment of Theriogenology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
Abstract
We investigated the relationship between the persistence of uterine bacterial infections with cytologically determined endometritis and ovarian function in 65 postpartum Holstein cows. Vaginal mucus discharges were collected, and endometrialsmear samples (n = 130) were collected for cytological and bacteriological examinations from the cows at weeks 5 and 7 postpartum (pp). Blood samples were collected at weeks 3, 5 and 7 pp to determine plasma progesterone concentrations to monitor ovarian activity. According to the bacteriological examination, cows were classified into four groups.The first group (n=32; 49%) comprised cows negative for bacteria at weeks 5 and 7 pp. The second group (n=11; 17%) comprised cows with bacterial infections at week 5 ppbut that were clear of infection at week 7 pp. The third group (n=12; 19%) comprised cows without bacteria at week 5 ppbut that acquired an infection by week 7 pp. The fourth group (n=10; 15%) comprised cows with bacterial infections at weeks 5 and 7pp (persistence of infection). A positive correlation (P<0.001) was noted between the severity of cytologically determined endometritis, purulent vaginal discharge and the persistence of infection. Cows with persistent infections had a significantly (P< 0.01) prolonged luteal phase compared with cows without infection. In conclusion, the prevalence of cytologically determined endometritis and prolonged luteal phase were significantly increased in cows with persistent infections.
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Published Research Articles in International Journals 2014-2015
Life Sciences 2015 193–201(141)
Chemopreventive Effect of Leflunomide Against Ehrlich's Solid Tumor Grown in Mice: Effect on EGF and EGFR Expression and Tumor Proliferation
HodaI.Bahra, EmanA.Toraihb,EmanA.Mohammedb,HalaM.F.Mohammadc,EmanA.I.Alid,SawsanA. e Zaitone aDepartmentofBiochemistry,FacultyofVeterinaryMedicine,SuezCanalUniversity,Ismailia,Egypt b Genetics Unit,DepartmentofHistology andCell Biology, Facultyof Medicine,SuezCanalUniversity,Ismailia,Egypt C DepartmentofPharmacology,FacultyofMedicine,SuezCanal University, Ismailia, Egypt dDepartmentofHistology,FacultyofMedicine,SuezCanalUniversity,Ismailia,Egypt eDepartmentofPharmacology&Toxicology,FacultyofPharmacy,SuezCanalUniversity,Ismai lia41522,Egypt
Abstract
Aims: The current study aimed to examine the effect of leflunomide on tumoral expression of epidermal growth factor and its receptor (EGFR) in Ehrlich's ascites carcinoma (EAC) grown in mice. ii) Materials and methods: Mice were injected subcutaneously with EAC cells and allocated into four groups; Group i: EAC control group. Groups ii-iv: mice treated with leflunomide (3, 10 or 30mg/kg/day, p.o.), respectively. Pharmacologic treatments were initiated at day 8 and continued for 14days. iii) Key findings: Treatment with leflunomide evoked antitumor properties as indicated by reduction in tumor mass, histopathological score, number of intratumoral PCNA immunopositive nuclei. Leflunomide (3, 10 or 30mg/kg) exerted an anti-inflammatory effect as indicated by the reduction in serum tumor necrosis factor-α. Furthermore, leflunomide demonstrated anti-angiogenic activity which was expressed as a decline in serum vascular endothelial growth factor and down-regulation of intratumoral EGF protein and mRNA expression as well as EGFR expression in addition to suppression of immunostaining for the endothelial marker, CD31. iv) Significance: Taken together, the present results demonstrated that leflunomide possessed anti- angiogenic and anti-proliferative activity against EAC solid tumors that might be correlated to down regulation of EGF and EGFR. Further, the current data indicated that leflunomide may have utility in the management of human cancer. Pag 20
Published Research Articles in International Journals 2014-2015
Journal of HELMINTHOLOGY 2015 1-8
The life cycle of HexangiumsiganiGoto& Ozaki, 1929(Digenea: Microscaphidiidae) from the Red Sea E.M Youssef3 3 Department of Parasitology, Fuclty of Vetrinary Medicine, Suez Canal University, Egypt.
Abstract
The microscaphidiidHexangiumsigani Goto&Ozaki,1929 was found in the intestine of Siganusrivulatus, a siganid fish permanently resident in a lagoon within the mangrove swamps on the Egyptian coast of the Gulf of aqaba. Intra-molluscan stages of this trematode(mother sporocysts, redia and cercariae) were not found in the gonads and digestive gland of Nassariuspullus (Gastropoda: Nassariidae), a common snail in the same lagoon. Consequently, the life cycle of H.sigani was elucidated under natural conditions: eggs are directly ingested by the snail; mother sporocysts and rediae reach maturity 5-7 and 16-17 weeks post-infection; rediae contain 18-26 developing cercariae; fully developed cercariae are monostome, without penetration glands, emerge from the snail during the night 18-19 weeks post-infection and rapidly encyst on aquatic vegetation(there is no second intermediate host); encysted metacercariae are not progenetic; 2-day-old metacercariae encysted on filamentous algae fed to S. rivulatusdeveloped into fully mature worms 5-6weeks post-infection. The cycle was completed in about 24 weeks. The intra molluscan stages are very similar to those of DictyangiumchelydraeStunkard, 1943, the only described intra molluscan stages of any microscaphidiid. However, they are also similar to those of the family Mesometridae. The present study ofH.siganidescribes the first complete microscaphidiid life cycle, and implicitly supports the phyelogenetic relationship of this family with the mesometridae inferred from molecular phylogenetic studies.
Pag 21
Published Research Articles in International Journals 2014-2015
Pharmacognosy Magazine 2015 )42(93-101
Toxicological and biochemical studies on Schinusterebinthifolius concerning its curative and hepatoprotective effects against carbon tetrachloride-induced liver injury. Abdou RH1, Saleh SY2, Khalil WF3.
1Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, University of Suez Canal, Ismailia 41522, Egypt. 2Department of Biochemistry, Faculty of Veterinary Medicine, University of Suez Canal, Ismailia 41522, Egypt. 3Department of Pharmacology, Faculty of Veterinary Medicine, University of Suez Canal, Ismailia 41522, Egypt.
Abstract
The present study was conducted to investigate the curative and protective effects of Schinusterbenthifoliusethanolic extract against CCl4 -induced acute hepatotoxicity in rats. MATERIALS AND METHODS: S. terbenthifolius extract was orally administered in a dose of 350 mg dried extract/kg b.wt. before and after intoxication with CCl4 for curative and protective experiments, respectively. A group of hepatotoxicity indicative enzymes, oxidant-antioxidant capacity, DNA oxidation, and apoptosis markers were measured. RESULTS: CCl4 increased liver enzyme leakage, oxidative stress, hepatic apoptosis, DNA oxidation, and inflammatory markers. Administration of S. terebinthifolius, either before or after CCl4 intoxication, significantly decreased elevated serum liver enzymes and reinstated the antioxidant capacity. Interestingly, S. terebinthifolius extract inhibited hepatocyte apoptosis as revealed by approximately 20 times down-regulation in caspase-3 expression when compared to CCl4 untreated group. On the other hand, there was neither protective nor curative effect of S. terebinthifolius against DNA damage caused by CCl4. CONCLUSION: The present study suggests that S. terebinthifolius extract could be a substantially promising hepatoprotective agent against CCl4 toxic effects and may be against other hepatotoxic chemical or drugs.
Pag 22
Published Research Articles in International Journals 2014-2015
J. Vet. Med. Sci. 2015 )10(1331–1334
Oral pharmacokinetics of acetaminophen to evaluate gastric emptying profiles of Shiba goats
Mohamed ELBADAWY1,2), Kazuaki SASAKI1), Yuji MIYAZAKI1), Mohamed ABOUBAKR2), WaleedFathy KHALIL3) and Minoru SHIMODA1)
1) Laboratory of Veterinary Pharmacology, Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183–8509, Japan 2) Pharmacology Department, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, Elqaliobiya, 13736, Egypt. 3) Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.
Abstract
The pharmacokinetics of acetaminophen was investigated following oral dosing to Shiba goats in order to evaluate the properties of gastric emptying. Acetaminophen was intravenously and orally administered at 30 mg/kg body weight to goats using a crossover design with a 3-week washout period. The stability of acetaminophen in rumen juice was also assessed. Acetaminophen concentrations were measured by HPLC. Since acetaminophen was stable in rumen juice for 24 hr, the extremely low bioavailability (16%) was attributed to its hepatic extensive first-pass effect. The mean absorption time and absorption half-life were unexpectedly short (4.93 and 3.35 hr, respectively), indicating its marked absorption from the forestomach, which may have been due to its smaller molecular weight. Therefore, acetaminophen was considered to be unsuitable for evaluating gastric emptying in Shiba goats.
Pag 23
Published Research Articles in International Journals 2014-2015
International journal of morphology 2015 (1):267-274
Histology and Scanning Electron Microscopy of the Lower Respiratory Tract in the Adult Red Fox (Vulpesvulpes)
Eid A. Moussa* & Said A. Hassan*
*Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
Abstract
The study was conducted on a total of three adult healthy freshly killed red foxes of both sexes weighing about 4- 6 kg collected from AbouRawwash, Giza, Egypt. The wall of trachea and bronchi formed of mucosa, submucosa and adventitia. The mucosa formed of pseudostratified columnar epithelium and lamina propria. The respiratory epithelium composed of tall columnar ciliated, goblet cells, basal cells, and neuroendocrine cells. Goblet cells account for about 20 to 30% of cells in the more proximal. Basal cells are relatively small triangular cells whose bases are attached to the basement membrane. The primary bronchiole is lined by simple columnar nonciliated to cuboidal epithelium containing some bronchiolar exocrine cells and some goblet cells. Neuroendocrine cells constitute about 4 to 5% of bronchial epithelial cells, attached at their bases to the basement membrane and have tapering apices. The lamina propria consists principally of a network of capillaries, a meshwork of connective tissue fibers continuous with the basement membrane. The submucosa formed of connective tissue elements and blood vessels and devoid of Tracheobronchial glands in red fox. The adventitia contain tracheal cartilage and muscle in trachea and bronchial cartilages and muscles in bronchi. Tracheal muscle is transverse bundles attached to the outer perichondrium. In the bronchi, muscle is organized in transverse bundles close to the epithelium adjacent to the lamina propria and longitudinal bundles close to the cartilages. Alveoli are demarcated by septa composed of a continuous layer of epithelial cells overlying a thin interstitium. The epithelial cells consist principally of type I and type II pneumocytes. SEM revealed that, the mucosal surface of both trachea and bronchi was completely covered by cilia. There were few glandular openings or goblet cell.
Pag 24
Published Research Articles in International Journals 2014-2015
Journal of biological chemistry 2015 (21(:13605-21
Crucial genes and pathways in chicken germ stem cells differentiation
Ahmed KamelElsayed 1, 4†,
1. College of Animal Science and Technology, Yangzhou University, Yangzhou, China 3 Anatomy and Embryology department, College of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
Abstract
Male germ cell differentiation is a subtle and complex regulatory process. Currently, its regulatory mechanism is still not fully understood. In our experiment, we performed the first comprehensive genome and transcriptome-wide analyses of the crucial genes and signaling pathways in three kinds of crucial cells (embryonic stem cells, primordial germ cell, and spermatogonial stem cells) that are associated with the male germ cell differentiation. We identified thousands of differentially expressed genes in this process, and from these we chose 173 candidate genes, of which 98genes were involved in cell differentiation, 19 were involved in the metabolic process, and 56 were involved in the differentiation and metabolic processes, like GAL9, AMH, PLK1, and PSMD7 and so on. In addition, we found that 18 key signaling pathways were involved mainly in cell proliferation, differentiation, and signal transduction processes like TGF-β, Notch, and Jak-STAT. Further exploration found that the candidate gene expression patterns were the same between in vitro induction experiments and transcriptome results. Our results yield clues to the mechanistic basis of male germ cell differentiation and provide an important reference for further studies.
Pag 25
Published Research Articles in International Journals 2014-2015
Parasites & Vectors 2015 8: 501
Molecular detection of vector-borne agents in dogs from ten provinces of China
Heba S. El-Mahallawy
Faculty of Veterinary Medicine, Suez Canal University
Abstract
BACKGROUND: Although many vector-borne agents are potential zoonoses and cause substantial morbidity and mortality in dogs worldwide, there are limited data on these organisms in dogs of China. METHODS: Quantitative PCRs for vector-borne agents were performed to investigate their prevalences in convenience whole blood samples obtained from 1114 dogs from 21 veterinary clinics and a commercial dog breeding facility in ten provinces of China. In addition, the PCRs were performed on 146 Rhipicephalussanguineussensolato and 37 Linognathussetosus collected from dogs in the commercial dog breeding facility. RESULTS:DNAs of Babesiagibsoni and B. vogeli (1.2 %), Ehrlichiacanis (1.3 %), Hepatozooncanis (1.8 %) and Theileriaorientalis (0.1 %) or a closely related organism were detected in the bloods of the dogs studied, and Babesiavogeli (3.4 %) and Ehrlichiacanis (4.1 %) in R. sanguineussensolato. The qPCRs for Anaplasma spp., Dirofilariaimmitis and Leishmania spp. were negative for all blood samples, ticks and lice. At least one vector- borne agent was found in dogs from 5 of the 10 provinces investigated in this study. Overall, 4.4 % (49/1117) of the dogs studied were positive for at least one vector-borne agent with the prevalence being highest in the commercial breeding colony (24/97; 24.7 %). CONCLUSIONS: Our study confirms that B. vogeli, B. gibsoni, H. canis, and E. canis occur in China. Also, we present evidence that T. orientalis or a closely related organism can infect dogs.
Pag 26
Published Research Articles in International Journals 2014-2015
Cytokine 2015 (73(:283–287
Tumor Necrosis Factor-a, Interleukins-12(p40), 6, and 10 levelsin cerebrospinal fluid and outcome prediction in Ossimi sheepwith encephalitic listeriosis.
Ossama A. Abdlla a, Mohamed E. Elboshyb,d, Engy F. Reisha b, Hossam A. Gadlla b, Sabry A. El-Khodery c,⇑ a Department of Clinical Pathology, Faculty of Veterinary Medicine, Suez Canal University, Egypt b Department of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, 35516, Egypt c Department of Internal Medicine, Infectious and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt d Department of Laboratory Medicine, Faculty of Applied Medical Sciences, AUM, Al-qura University Makka, Saudi Arabia
Abstract
Encephalitic listeriosis in sheep is a life-threatening disease. However, little is known about the cytokineresponse and their predictive value in this disease. The aim of present study was to assess the prognosticsignificance of Tumor Necrosis Factor-a (TNF-a), Interleukin-12(p40) (IL-12 p40), Interleukin-6 (IL-6),and Interleukin 10 (IL-10) levels in cerebrospinal fluid (CSF) in sheep with encephalitic listeriosis. Fifty-nine ewes in 14 flocks were diagnosed clinically as having listeriosis. CSF was collected andsubjected to bacteriological examination and estimation of selected cytokines. Twenty-eight ewes wereconfirmed to be infected with Listeria monocytogenes. Based on antimicrobial sensitivity test, sheep weretreated and the outcome was recorded as survivors (n = 10) and non-survivors (n = 18). Cutoff points forCSF cytokines were determined by Receiver operating characteristic analysis (ROC). Association betweenlevels of CSF cytokines and outcome of listeriosis was assessed by logistic regression. TNF-a, IL-6 andIL-12(p40) levels as well as TNF-a/IL-10 ratio were significantly higher in non-survivors than survivors(p = 0.002, 0.0021, 0.0033, and 0.001, respectively). However, IL-10 level was significantly lower innon-survivors than survivors (p = 0.0058). ROC analysis revealed that IL-6 and TNF-a/IL-10 ratio hadthe highest AUC values (0.98, 0.984, respectively). Final multivariate logistic regression model showedthat TNF-a/IL-10 ratio was the only variable that has predictive value for mortality in diseased sheep(p: 0.001; OR: 7.2; 95% CI: 5.7–9.8). TNF-a showed a positive correlation with IL-12b (r = 0.917) andIL-6 (r = 0.965). IL-12 (p40) showed also a positive correlation with IL-6 (r = 0.906). However, IL-10showed a negative correlation with TNF-a (r = _0.915), IL-12(p40) (r = _0.790), and IL-6 (r = _0.902). In conclusion, TNF-a/IL-10 ratio may provide predictive information about outcome of encephaliticlisteriosis in sheep.
Pag 27
Published Research Articles in International Journals 2014-2015
Journal of Animal and Feed Sciences 2015 (24(:302–307
Effect of probiotic strains mixture administration on serum interleukins concentration lymphocyte proliferation and DNA damage in rams
H.M.A. Abdelrazek1, M S. Yusuf2, S.A. Ismail4 and R.A.R. Elgawish3,5
Suez Canal University, Faculty of Veterinary Medicine, Ismailia 41522, Egypt 1 Department of Physiology, 2 Department of Nutrition and Clinical Nutrition, 3 Department of Forensic Medicine and Toxicology 4 Zagazig University, Faculty of Veterinary Medicine, Department of Clinical Pathology, Zagazig 44111, Egypt.
Abstract
Probiotics are live bacteria that could exert health beneficial effects; however, their immunological effects in sheep are still scarce. The aim of this study was to investigate the immunomodulatory role of probiotics in 12 rams weighing 30–35 kg. The animals were divided into 2 groups (6 animals each), one group served as a control and the other as a probiotic treated one. Treated rams were given orally commercial probiotic strains (Sacharomycescerevisiae, Lactobacillus acidophilus, Lactobacillus plantarum and Enterococcus faecalis) mixture in a dose of 10 g/head/day for one month. Blood samples were collected from control and treated rams at the end of the whole experimental period. Total and differential leukocyte counts (TLC and DLC), interleukin 10 (IL-10) and 12 (IL-12) serum concentration, IL-10/IL-12 ratio, lymphocyte transformation and DNA damage (comet assay) were determined. TLC in the blood of treated rams decreased significantly (P = 0.009), the serum levels of IL-10 and IL-12 were not significantly changed, while IL- 10/IL-12 ratio was significantly (P = 0.03) increased after probiotic mixture administration in rams. The administration of probiotic mixture significantly (P = 0.0001) enhanced the transformation of lymphocytes in rams‟ blood. After probiotic mixture administration, the percent of DNA in the comet tail declined significantly (P = 0.02) from 2.75% to 2.57%. The present study demonstrated that leukocytes, cytokine production, lymphocyte transformation and DNA integrity were differentially influenced by the tested probiotic strains mixture. The biological importance of specific effects of probiotics in sheep remains to be determined.
Pag 28
Published Research Articles in International Journals 2014-2015
Journal of Immunopharmacology and Immunotoxicology
2015 (2), 126-139
Anti-inflammatory and immunomodulatory effects of Spirulinaplatensisin comparison to Dunaliellasalina in acetic acid-induced rat experimental colitis.
Mohamed M. Abdel-Daim1, Sameh M. Farouk2, Fedekar F. Madkour3, and Samar S. Azab4
1Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt, 2Cytology & Histology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt, 3Marine Science Department, Faculty of Science, Port Said Univeristy, Port Said, Egypt, and 4Pharmacology & Toxicology Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
Abstract
Context: Spirulinaplatensis (SP) is used as a source of protein and vitamin supplement in humans without any significant side-effects. Dunaliellasalina (DS) is also regarded as one of the richest natural producers of carotenoid, thus used as a source of antioxidants to protect cells from oxidative damage. Objective: The aim of the present study is to compare the ameliorative effect of Spirulina and Dunaliella in experimental colitis. Materials and methods: Spirulina and Dunaliella were investigated at the same dose of 500 mg/kg body weight for their modulatory effect against acetic-acid induced ulcerative colitis (UC) in rats. The colonic lesion was analyzed by examining macroscopic damage, bloody diarrhea scores, colon weight/length and change in body weight of tested rats. Colon lipid peroxidation and oxidative stress markers were examined by evaluating malondialdehyde (MDA), protein carbonyl (PCO), catalase (CAT), reduced glutathione (GSH) and superoxide dismutase (SOD). Colon inflammatory markers; myeloperoxidase (MPO) and prostaglandin (PGE2) as well as proinflammatory cytokines; tumor necrosis factor (TNF-a) and interleukins (IL-1b, IL-6) were also studied. Results: The colonic mucosal injury, biochemical and histopathologic results suggest that both SP and DS exhibit significant modulatory effect on acetic acid-induced colitis in rats, which may be due to a significant increase of antioxidant enzymes activity and significant inhibition of lipid peroxidation and inflammation markers. Discussion: Results showed that in comparison to Sulfasalazine, SP exhibited better therapeutic and safety profile than DS against acetic acid-induced UC. Conclusion: This study suggests potential benefits of SP and DS in an experimental model of colitis.
Pag 29
Published Research Articles in International Journals 2014-2015
Small Ruminant Research 2015 (133), 1-6
Investigation the mRNA expression of KISS1 and localization ofkisspeptin in the testes of Shiba goats and its relationship with thepuberty and steriodogenic enzymes.
Haney Samira,b,KentaroNagaokaa, AlyKarenc, EmanAhmedd, Mohamed El Sayedb,GenWatanabea aLaboratory of Veterinary Physiology, Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan. bDepartment of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt cDepartment of Theriogenology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 13561, Egypt dDepartment of Pharmacology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
Abstract
The objectives of the present study were to investigate the mRNA expression of KISS1 and localization ofkisspeptin in the testes of Shiba goats. Additionally, its relationship with the puberty was investigated.Testes from prepubertal (1 month; n = 3), and postpubertalShiba goats (17 ± 1.5 months; n = 4) werecollected by surgical castration. Plasma testosterone (T) and estradiol (E2) were measured just beforethe castration using radioimmunoassay. Testicular expression of mRNAs encoding KISS1, GPR54 and thefollowingsteroidogenic enzymes: cytochrome P450 side-chain cleavage (P450scc;CYP11A1 gene), 3beta- hydroxysteroid dehydrogenase (3ˇHSD; HSD3B1 gene), cytochrome P450, 17alpha- hydroxylase/17, 20lyase (P450c17; CYP17 gene), and cytochrome P450 aromatase (P450arom; CYP19 gene) were quantifiedby real time PCR. The localization of kisspeptin in testes was determined by immunohistochemistry.Results revealed high plasma T and E2 in postpubertal goats compared to prepubertal goats. High signif-icant increases (P < 0.05) of mRNAs encoding KISS1, GPR54, CYP11A1, HSD3B1, CYP17, and CYP19 genes inthe testes of postpubertal goats compared to prepubertal goats. In addition, kisspeptin was immunolo-calized in Leydig cells of the testis. The staining was mild in the interstitial cells of the prepubertalgoatswhile strong staining was found in the postpubertal goats. In conclusion: Leydig cells are responsible forkisspeptin production in the goat testis and this expression is puberty dependent.
Pag 30
Published Research Articles in International Journals 2014-2015
Journal of RENAL FAILURE 2015 (37), 1198-1207
Protective role of polyphenols from Bauhinia hookeriagainst carbon tetrachloride-induced hepato- and nephrotoxicity in mice
Eman Al-Sayeda, Mohamed M. Abdel-Daimb, Omnia E. Kilanyc, MaaritKaronend&JariSinkkonend
Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo, Egypt, b Department of Pharmacoloy, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt, c Department of Clinical Pathology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt, and d Laboratory of Organic Chemistry and Chemical Biology, Department of Chemistry, University of Turku, Turku, Finland
Abstract
The hepatoprotective and nephroprotective activity of a polyphenol-rich fraction (BHPF) obtained from Bauhinia hookeri was investigated against CCl4-induced acute hepatorenal toxicity in mice. BHPF was administered (100, 200 and 400 mg/kg/day) for 5 days, then CCl4 was administered. BHPF pretreatment significantly (p50.001) inhibited the CCl4- induced increase in ALT, AST, ALP, LDH, total bilirubin, cholesterol, creatinine, uric acid, urea and malondialdehydein a dose-dependent manner. In contrast, BHPF pretreatment markedly increased the contents of glutathione and superoxide dismutase in the liver and kidney tissues, indicating the strong invivo antioxidant activity of BHPF. Pretreatment with BHPF preserved the hepatic architecture and conferred marked protection against necrosis and ballooning degeneration. Pretreatment with BHPF reduced the inflammatory cell aggregation and degenerative changes in the lining epithelium of the kidney tubules. It can be concluded that BHPF has a remarkable hepato- and nephroprotective activity by enhancing the antioxidant defense status, reducing lipid peroxidation and protecting against the histopathological changes induced by CCl4 in the liver and kidney tissues.
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Published Research Articles in International Journals 2014-2015
Kidney international 2015 (89), 317–326
Histone deacetylase mediated silencing of AMWAP expression contributes to cisplatin nephrotoxicity.
Rania Hamad
Department of Pathology, Suez Canal University, Ismailia, Egypy
Abstract
Cisplatin-induced acute kidney injury is a serious problem in cancer patients who receive chemotherapy for the treatment of solid tumors. Currently, there are no therapies available to treat or prevent cisplatin nephrotoxicity. Agents that can promote cisplatin anti-tumor activity and at the same time suppress nephrotoxicity is a desirable form of treatment. Since histone deacetylase (HDAC) inhibitor is known to augment cisplatin anti-tumor activity, we tested whether HDAC inhibitors can prevent cisplatin-induced nephrotoxicity and determined the underlying mechanism. Administration of cisplatin up-regulated several HDACs in the kidney. Inhibition of HDAC with a clinically used inhibitor, trichostatin A (TSA), suppressed cisplatin-induced kidney injury, inflammation and epithelial cell apoptosis. Moreover, TSA up-regulated a novel anti-inflammatory protein called activated microglia/macrophage WAP domain protein (AMWAP) in epithelial cells, which was enhanced with cisplatin treatment. Interestingly, HDAC1 and -2 specific inhibitors are sufficient to up-regulate AMWAP potently in epithelial cells. Administration of recombinant AMWAP or epithelial cell-specific overexpression reduced cisplatin- induced kidney dysfunction. Moreover, AMWAP treatment suppressed epithelial cell apoptosis, and siRNA-based knockdown of AMWAP expression abolished TSA-mediated suppression of epithelial cell apoptosis in vitro. Our data suggest that HDAC-mediated silencing of AMWAP may contribute to cisplatin nephrotoxicity. Therefore, HDAC1 and -2 specific inhibitors or AMWAP could be a useful therapeutic agent for the prevention of cisplatin nephrotoxicity.
Pag 32
Published Research Articles in International Journals 2014-2015
Ecotoxicology and Environmental Safety 2015 (111), 146-152
Antagonistic activity of dietary allicin against deltamethrin-induced oxidative damage in freshwater Nile tilapia; Oreochromisniloticus
Mohamed M.Abdel-Daima.NevienK.M.Abdelkhalekb.AhmedM.Hassanc aPharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt bDepartment of Internal Medicine, Infectious and Fish Diseases, Faculty of Veterinary Medicine, El Mansoura University, Egypt cDepartment of Hygiene, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt
Abstract
Allicin, the main biologically active component of garlic clove extracts, has been evaluated for its′ efficacy in preventing deltamethrin-induced oxidative damage in Nile tilapia; Oreochromisniloticus. Fish were fed on 2 different doses of 0.5 g and 1 g of allicin/ kg diet for 28 days. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), cholesterol, urea, uric acid, creatinine, total protein, albumin and globulin were estimated. Moreover, the level of malonaldehyde (MDA) was analyzed as a lipid peroxidation marker. In addition, reduced glutathione (GSH), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) were analyzed as antioxidant biomarkers in liver, kidney and gills. Results show that deltamethrinsubacute intoxication (1.46 µg/L for 28 days) increased serum AST, ALT, ALP, cholesterol, urea, uric acid, creatinine and tissue MDA. At the same time, serum total protein and albumin as well as tissue level of GSH, GSH-Px, SOD and CAT were reduced. Allicin supplemented diets enhanced all the altered serum biochemical parameters as well as tissues′ lipid peroxidation and antioxidant biomarkers in a dose-dependent manner. The results suggest that feeding allicin can ameliorate deltamethrin-induced oxidative stress and might have some therapeutic properties to protect Nile tilapia on subacutedeltamethrin toxicity.
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Published Research Articles in International Journals 2014-2015
Canadian Journal of Physiology and Pharmacology 2015 (1): 45-51
Synergistic Protective Role of Mirazid (CommiphoraMolmol) and Ascorbic Acid againstTilmicosin-Induced Cardiotoxicity in Mice.
Mohamed M. Abdel-Daim,a Emad W. Ghazy,b MostafaFayeza aPharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt. bDepartment of Clinical Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
Abstract
Tilmicosin (TIL) is a long-acting macrolide antibiotic approved for the treatment of cattle with Bovine Respiratory Disease. However, overdose of TIL has been reported to induce cardiotoxicity. The purpose of our experiment was to evaluate the protective effects of Commiphora molmol (mirazid (MRZ); myrrh) and (or) ascorbic acid (AA) against TIL- induced cardiotoxicity in mice. MRZ and AA were orally administered using stomach gavage, either alone or in combination for 5 consecutive days, followed with a single TIL overdose. TIL overdose induced a significant increase in serum levels of cardiac damage biomarkers (AST, LDH, CK, CK-MB, and cTnT), as well as cardiac lipid peroxidation, but cardiac levels of antioxidant biomarkers (GSH, SOD, CAT, and TAC) were decreased. Both MRZ and AA tended to normalize the elevated serum levels of cardiac injury biomarkers. Furthermore, MRZ and AA reduced TIL-induced lipid peroxidation and oxidative stress parameters. MRZ and AA combined produced a synergistic cardioprotective effect. We conclude that myrrh and (or) vitamin C administration minimizes the toxic effects of TIL through their free-radical-scavenging and potent antioxidant activities.
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Published Research Articles in International Journals 2014-2015
ONCLOGY REPORTS 2015 (1): 495–503
3-(2-Bromoethyl)-indole inhibits the growth of cancer cells and NF-κB activation.
RAMY ELGENDY Department of Pharmacology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
Abstract
Indole-3-carbinol (I3C) and diindolylmethane (DIM), found in cruciferous vegetables, have chemopreventive and anticancer properties. In the present study, 14 substituted indoles were tested for activity against SW480 colon cancer cells. Among these, 3-(2-bromoethyl)- indole, named BEI-9, showed the greatest inhibition. The effects of BEI-9 on cancer cells were analyzed by MTS and CellTiter-Glo assays for effects on cell viability, by microscopy for phenotypic changes, by scratch wound assays for effects on migration, by flow cytometry for changes in the cell cycle, by immunoblotting for cyclin D and A to assess effects on cell cycle regulation, and by NF-κB reporter assays for effects on basal and drug-induced NF-κB activation. BEI-9 inhibited the growth of SW480 and HCT116 colon cancer cells at concentrations of 12.5 and 5 µM, respectively. BEI-9 also inhibited cell motility as determined with scratch wound assays, and reduced the levels of cyclin D1 and A. Furthermore, in reporter cells, BEI-9 (0.8 µM) inhibited basal and induced NF-κB activation and increased cell death when combined with the cytokine TNFα or the drug camptothecin (CPT), both of which activate NF-κB. Preliminary experiments to identify a safe dose range for immunodeficient mice showed that BEI-9, administered intraperitoneally, was tolerable at doses below 10 mg/kg. Thus, BEI-9 and other indole derivatives may be useful in chemoprevention or as chemosensitizers. Since NF-κB activation is implicated in carcinogenesis and in reducing sensitivity to anticancer drugs, BEI-9 should be investigated in combination with drugs such as CPT, which activate NF- κB.
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Published Research Articles in International Journals 2014-2015
Environmental Science and Pollution Research 2015 (22): 3023–3031
Pharmacodynamic interaction of Spirulinaplatensis and deltamethrin in freshwater fish Nile tilapia, Oreochromisniloticus: impact on lipid peroxidation and oxidative stress Nevien K. M. Abdelkhalek&Emad W. Ghazy&Mohamed M. Abdel-Daim
Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt
Abstract
Spirulinaplatensis (SP) is one of the most commonly used dietary supplements in human and many animal species, including fish. Recently, it has gained more attention in fish not only for its growth-promoting and immunomodulatory effects but also for its antioxidant potential. The present study was conducted to investigate the protective role of two different dietary levels of SP on freshwater Nile tilapia; Oreochromisniloticus exposed to subacutedeltamethrin (DLM) intoxication. Spirulina was supplemented at levels of 0.5 and 1 % in the diet along with DLM at a concentration of 1.46 μg/l for 28 days. Serum biochemical parameters, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein, albumin, cholesterol, urea, uric acid and creatinine, were estimated. In addition, the level of malondialdehyde (MDA) was analysed as a lipid peroxidation marker. Reduced glutathione (GSH) content and glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) activities were analysed as antioxidant biomarkers in liver, kidney and gills. The results revealed that DLM intoxication increased serum AST, ALT, ALP, cholesterol, urea, uric acid, creatinine and tissue MDA, while decreased serum total protein and albumin as well as tissue GSH level and GSH-Px, SOD and CAT activities. SP supplementation at the two tested levels enhanced all altered serum biochemical parameters as well as tissue lipid peroxidation and antioxidant biomarkers. Therefore, it could be concluded that SP administration could minimize DLMinduced toxic effects by its free radical scavenging and potent antioxidant activity.
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Published Research Articles in International Journals 2014-2015
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Published Research Articles in International Journals 2014-2015
Journal of Controlled Release 2014 (189): 19-24
In vivo proof of concept of oral insulin delivery based on a co- administration strategy with the cell-penetrating peptide penetratin
El-SayedKhafagy
Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Suez Canal University, Ismailia 415-22, Egypt
Abstract
Oral delivery of insulin is blocked by low intestinal absorption caused by the poor permeability of insulin across cellular membranes and the susceptibility to enzymatic degradation in the gastrointestinal tract. Cell-penetrating peptides (CPPs) have been investigated for a number of years as oral absorption enhancers for hydrophilic macromolecules. Penetratin, a cationic and amphipathic CPP, effectively enhances insulin absorption and we were able to alleviate the enzymatic barrier by using the enzymatic resistant D-form of penetratin. In this study, mice were dosed orally with a physical mixture of insulin and penetratin. Blood glucose concentrations were measured and a pharmacological availability (PA) of 18.2% was achieved in mice dosed with insulin and D-penetratin. Following the promising data, we investigated the degradation parameters of insulin and penetratin in rat intestinal fluid. As expected, L-penetratin was degraded rapidly whereas D-penetratin had a halflife of 67 ± 7 min in 10-fold diluted gastrointestinal fluid. Insulin degradation was slowed by the presence of penetratin in intestinal fluid. The half-life of insulin increased from 24.9 ± 4.5 min to 55.6 ± 14 min and 90.5 ± 11.8 min in the presence of L- and D-penetratin respectively. In conclusion, both Land D-penetratin acted as oral absorption enhancers at select CPP concentrations for insulin and the current study is the first solid evidence of pharmacological activity of oral insulin delivery systems based on non-covalent intermolecular interactions with penetratin.
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Published Research Articles in International Journals 2014-2015
Analyst 2015 )140( 8148-8156
Quinones as novel chemiluminescent probesforthe sensitive and selective determination ofbiothiols in biologicalb fluids
Mohamed Saleh Elgawish Medicinal Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt
Abstract
Altered plasma aminothiol concentrations are thought to be a valuable risk indicator and are interestingly utilized for routine clinical diagnosis and for the monitoring of various metabolic disorders and human diseases, and accordingly there is a need for an accurate and reliable assay capable of simultaneously determining aminothiols including glutathione (GSH), N-acetylcysteine (NAC), homocysteine (Hcys), and cysteine (Cys) in human plasma. Herein, a highly sensitive, selective, and very fast HPLC– chemiluminescence (HPLC–CL) coupled method is reported, exploiting for the first time the strong nucleophilicity and high reactivity of aminothiols toward quinones for a CL assay. The unique redox-cycling capability of quinone and/or Michael addition adducts, thioether–quinone conjugates, was utilized to establish a novel analytical method based on the reaction of adducts with dithiothreitol (DTT) to liberate reactive oxygen species (ROS), which are detected by using a luminol-CL assay. Specimen preparation involved the derivatization of aminothiols with menadione (MQ) for 5 minutes at room temperature. A unique green chemistry synthesis of thioether–quinones in HEPES buffer (pH 8.5) was introduced by using our reaction methodology without needing any hazardous organic solvent or catalyst. The aminothiol–MQ adducts were separated using solid-phase extraction followed by isocratic elution on an ODS column. Linearity was observed in the range of 2.5–500, 5–500, 10–1500, and 20–2000 nM with detection limits (S/N of 3) of 3.8, 4.2, 8, and 16 (fmol per injection) for GSH, NAC, Hcys, and Cys, respectively. The method was successfully applied for the selective determination of aminothiols in human plasma from healthy people and patients with rheumatic arthritis and diabetes mellitus. The obtained results postulated the usefulness of our method for investigating the relationship between aminothiol metabolism and related human disorders.
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Published Research Articles in International Journals 2014-2015
Angiogenesis 2015 (18): 137–150
Candesartan stimulates reparative angiogenesis in ischemic retinopathy model: role of hemeoxygenase-1 (HO-1)
Mona F. El-Azab
Faculty of PharmacySuez Canal UniversityIsmailiaEgypt
Abstract
Ischemic diseases such as stroke and proliferative retinopathy are characterized by hypoxia-driven release of angiogenic factors such as vascular endothelial growth factor (VEGF). However, revascularization of the ischemic areas is inadequate, resulting in impaired neuro-vascular function. We aim to examine the vascular protective effects of candesartan, an density, number of tip cells, and perfusions of capillaries were assessed. Activation of Muller glial cells and levels of peroxynitrite, VEGF, VEGFR2, inducible nitric oxide synthase, hemeoxygenase-1 (HO-1) were assessed. Proangiogenic effects of candesartan were examined in human endothelial cells (EC) that were cultured in normoxia or hypoxia and transduced with siRNA against HO-1. Candesartan (1 mg/kg) and (10 mg/kg) decreased hypoxia-induced neovascularization by 67 and 70 %, respectively. Candesartan (10 mg/kg) significantly stimulated the number of tip cells and physiological revascularization of the central retina (45 %) compared with untreated pups. The effects of candesartan coincided with reduction of hypoxia-induced Muller glial activation, iNOS expression and restoration of HO-1 expression with no significant change in VEGF levels. In vitro, silencing HO-1 expression blunted the ability of candesartan to induce VEGF expression under normoxia and VEGFR2 activation and angiogenic response under both normoxia and hypoxia. These findings suggest that candesartan improved reparative angiogenesis and hence prevented pathological angiogenesis by modulating HO-1 and iNOS levels in ischemic retinopathy. HO-1 is required for VEGFR2 activation and proangiogenic action of candesartan in EC. Candesartan, an FDA-approved drug, could be repurposed as a potential therapeutic agent for the treatment of ischemic diseases.
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Published Research Articles in International Journals 2014-2015
International Journal of Biological Macromolecules 2014 (70): 222-229
Halophilic alkali- and thermostable amylase from a novel polyextremophilic Amphibacillus sp. NM-Ra2
Noha M.Mesbah Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt
Abstract
Extracellular gluco-amylo-pullulanase from Amphibacillus sp. NM-Ra2 was purified to homogeneity by ethanol precipitation, anion exchange chromatography and gel filtration chromatography. Molecular mass of the enzyme was 50 kDa (SDS-PAGE). The enzyme showed maximal activity at 1.9 M NaCl, pH50 °C 8.0 and 54 °C and was active from 0 to 4.3 M NaCl and 37 to 65 °C. The enzyme was inhibited by EDTA and was stable and active 2+ in the presence of PMSF, DTT, H2O2, Triton-X-100, Tween 20 and Tween 80. Ca is inessential for activity. The amylase was stimulated with K+ and inhibited with Cu2+ and Mg2+. Hg2+, Zn2+ and Fe2+ had no effect on activity. Amylase was stable and active in the presence of ethanol, methanol and benzene (25%, v/v). The enzyme hydrolyzed linear and branched polysaccharides including pullulan, glycogen and amylopectin, and hydrolyzed raw wheat starch and raw corn starch (14.6% and 13.5% over 2 h). Amylase activity was inhibited by soluble starch concentrations greater than 0.3%. The major products of soluble starch hydrolysis were maltose and maltotriose. The amylase, being halophilic and alkali-thermostable, in addition to being resistant to surfactants, oxidizing agents and organic solvents, can find applications in the starch processing, pharmaceutical, food and paper/pulp industries.
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Published Research Articles in International Journals 2014-2015
Bioorganic Chemistry 2015 (60):1-12
Discovery of novel tricyclic pyrido [3′, 2′:4, 5] thieno [3, 2-d] pyrimidin-4- amine derivatives as VEGFR-2 inhibitors
Yasmine M.Abdel Aziza.Mohamed M.Saida.Hosam A.El Shihawya.Khaled A.M.Abouzidb aPharmaceutical Organic Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt bPharmaceutical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt
Abstract
In an effort to develop ATP-competitive VEGFR-2 selective inhibitors, a novel series of tricyclic pyrido[3′,2′:4,5]thieno[3,2-d]pyrimidin-4-amine derivatives were designed and synthesized. These compounds were characterized by IR, 1H NMR, 13C NMR, elemental and mass spectral analyses. Docking studies have given a partial insight into the molecular determinants of the activity of this novel series in VEGFR-2 kinase active site. Moreover, these compounds were assessed at 10 μM for their selective inhibitory activities over a panel of 6 human kinases, namely VEGFR-1/Flt-1, VEGFR-2/KDR, EGFR, CDK5/p25, GSK3α and GSK3β. Compound N-(4,6-dimethylthieno[2,3-b]pyridine)-7,9- dimethylpyrido[3′,2′:4,5]thieno[3,2-d]pyrimidin-4-amine (9d) exhibited the most potent and selective inhibitory activity against VEGFR-2/KDR over the six human kinases, with an
IC50 value 2.6 μM. The identification of this hit candidate could aid the design of new tricyclic-based VEGFR-2 kinase modulators.
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Published Research Articles in International Journals 2014-2015
Archives of Physiology and Biochemistry 2015 (121):13-18
Relation of myeloperoxidase-463G/A polymorphism with metabolic syndrome and its component traits in Egyptian women
Eman T. Mehanna1, Samy M. Saleh2, Noha M. Mesbah3 & Dina M. Abo-Elmatty4
1Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt. 2Department of Biochemistry, Faculty of Pharmacy,Suez Canal University, Ismailia, Egypt. 3Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt. 4Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
Abstract
Context: Myeloperoxidase is a heme protein secreted by activated macrophages and generates intermediates that oxidize lipoproteins. Myeloperoxidase-463G/A is a functional polymorphism involved in regulation of myeloperoxidase expression.
Objective: The aim of this study is to assess the relation of myeloperoxidase-463G/A polymorphism with metabolic syndrome and its component traits in Egyptian women from the Suez Canal area.
Methods: The study includes 100 healthy female subjects and 100 metabolic syndrome patients. The component traits of metabolic syndrome are determined and the genotypes of the polymorphisms assessed using the PCR-RFLP technique.
Results: There was no significant difference in the allele frequencies between the metabolic syndrome and control groups. However, the GA and AA genotypes were associated with lower total cholesterol, LDL-C, systolic and diastolic blood pressure in the patients.
Conclusion: Myeloperoxidase-463G/A polymorphism is not associated with the incidence of metabolic syndrome.
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Published Research Articles in International Journals 2014-2015
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2015 (141):278-286
Chemometric-assisted spectrophotometric methods and high performance liquid chromatography for simultaneous determination of seven β-blockers in their pharmaceutical products: A comparative study
Eman A.Abdel Hameed.Randa A.Abdel Salam.Ghada M.Hadad Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt
Abstract
Chemometric-assisted spectrophotometric methods and high performance liquid chromatography (HPLC) were developed for the simultaneous determination of the seven most commonly prescribed β-blockers (atenolol, sotalol, metoprolol, bisoprolol, propranolol, carvedilol and nebivolol). Principal component regression PCR, partial least square PLS and PLS with previous wavelength selection by genetic algorithm (GA-PLS) were used for chemometric analysis of spectral data of these drugs. The compositions of the mixtures used in the calibration set were varied to cover the linearity ranges 0.7– 10 μg ml−1 for AT, 1–15 μg ml−1 for ST, 1–15 μg ml−1 for MT, 0.3–5 μg ml−1 for BS, 0.1– 3 μg ml−1 for PR, 0.1–3 μg ml−1 for CV and 0.7–5 μg ml−1 for NB. The analytical performances of these chemometric methods were characterized by relative prediction errors and were compared with each other. GA-PLS showed superiority over the other applied multivariate methods due to the wavelength selection. A new gradient HPLC method had been developed using statistical experimental design. Optimum conditions of separation were determined with the aid of central composite design. The developed HPLC method was found to be linear in the range of 0.2–20 μg ml−1 for AT, 0.2– 20 μg ml−1 for ST, 0.1–15 μg ml−1 for MT, 0.1–15 μg ml−1 for BS, 0.1–13 μg ml−1 for PR, 0.1– 13 μg ml−1 for CV and 0.4–20 μg ml−1 for NB. No significant difference between the results of the proposed GA-PLS and HPLC methods with respect to accuracy and precision. The proposed analytical methods did not show any interference of the excipients when applied to pharmaceutical products.
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Published Research Articles in International Journals 2014-2015
Natural Product Research 2015 (29):593-601
Validated HPLC method for simultaneous estimation of khellol glucoside, khellin and visnagin in Ammi visnaga L. fruits and pharmaceutical preparations
Jihan M. Badr1, Ghada M. Hadad2, Khaled Nahriry3 & Hashem A. Hassanean4
1Department of Pharmacognsoy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt. 2Department of Pharmaceutical Analytical chemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt. 3Department of Pharmacognsoy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt. 4Department of Pharmacognsoy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
Abstract Tea bags including fruits of Ammi visnaga L. are used in Egypt as remedy for the treatment of kidney stones. Our study focuses on developing simple and rapid method utilising HPLC for quantitative estimation of khellol glucoside (KG), khellin (KH) and visnagin (VS) simultaneously. Their concentrations were determined in A. visnaga L. fruits at different developmental stages and in pharmaceutical formulations together with following up them during shelf life. Separation was accomplished using HPLC. Perfect resolution between KG, KH and VS was possible through using a mobile phase consisting of water:methanol:tetrahydrofuran (50:45:5, v/v/v). Peaks were detected at 245 nm. The suggested method for the determination of KG, KH and VS was successful in determining the analytes of interest without any interference of other compounds and matrix. All validation parameters were satisfactory and the procedure was relatively easy and fast as extracts are evaluated without previous steps of purification.
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Published Research Articles in International Journals 2014-2015
Journal of Chromatography A 2015 (1399):32-44
Development of a fast high performance liquid chromatographic screening system for eight antidiabetic drugs by an improved methodology of in- silico robustness simulation.
Randa A.Abdel Salam.Ghada M.Hadad Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
Abstract
Robustness of RP-HPLC methods is a crucial method quality attribute which has gained an increasing interest throughout the efforts to apply quality by design concepts in analytical methodology. Improvement to design space modeling approaches to represent method robustness was the goal of many previous works. Modeling of design spaces regarding to method robustness fulfils quality by design essence of ensuring method validity throughout the design space. The current work aimed to describe an improvement to robustness modeling of design spaces in context of RP-HPLC method development for screening of eight antidiabetic drugs. The described improvement consisted of in-silico simulation of practical robustness testing procedures thus had the advantage of modeling design spaces with higher confidence in estimated of method robustness. The proposed in-silico robustness test was performed as a full factorial design of simulated method conditions deliberate shifts for each predicted point in knowledge space with modeling error propagation. Design space was then calculated as zones exceeding a threshold probability to pass the simulated robustness testing. Potential design spaces were mapped for three different stationary phases as a function of gradient elution parameters, pH and ternary solvent ratio. A robust and fast separation for the eight compounds within less than 6min was selected and confirmed through experimental robustness testing. The effectiveness of this approach regarding definition of design spaces with ensured robustness and desired objectives was demonstrated.
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Published Research Articles in International Journals 2014-2015
Canadian Journal of Physiology and Pharmacology 2015 (8): 695-708
Boswellic acids synergize antitumor activity and protect against the cardiotoxicity of doxorubicin in mice bearing Ehrlich‟s carcinoma.
Shimaa A. Ali,a Sawsan A. Zaitone,b Yasser M. Moustafab aSuez Canal Authority hospital, Ismailia, Egypt. bDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.
Abstract
This study aimed to test whether boswellic acids add to the antitumor effects of doxorubicin against solid tumors of Ehrlich‟s ascites carcinoma (EAC) grown in mice, and to investigate the protective effects of boswellic acids against doxorubicin-induced cardiotoxicity. Sixty-four female Swiss albino mice bearing EAC solid tumors were distributed among 8 groups as follows: group 1, EAC control group; group 2, doxorubicin treatment group [mice were injected with doxorubicin (6 mg·(kg body mass)−1·week−1) for 3 weeks]; groups 3–5, these mice were treated with boswellic acids (125, 250, or 500 mg·kg−1·day−1), respectively; groups 6–8, these mice were treated with a combination of doxorubicin and boswellic acids (125, 250, or 500 mg·kg−1·day−1), respectively, for 3 weeks. The results indicated that boswellic acids synergized the antitumor activity of doxorubicin. Doxorubicin-treated mice showed elevated serum activities of lactate dehydrogenase and creatine kinase isoenzyme MB as well as cardiac malondialdehyde. Further, decreases in cardiac levels of reduced glutathione, superoxide dismutase, and catalase activities were observed. These effects were accompanied by an increase in cardiac expression of caspase 3. Thus, treatment with boswellic acids attenuated doxorubicin-evoked disturbances in the above-mentioned parameters, highlighting antioxidant and antiapoptotic activities. Therefore, boswellic acids could be potential candidates for ameliorating the cardiotoxicity of doxorubicin.
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Published Research Articles in International Journals 2014-2015
Planta Medica 2015 (05): 382-387
Antichlamydial Sterol from the Red Sea Sponge Callyspongia aff.implexa
Usama Ramadan Abdelmohsen1, 5, Cheng Cheng1, Anastasija Reimer2, Vera Kozjak- Pavlovic2, Amany K. Ibrahim3, Thomas Rudel2, Ute Hentschel1, RuAngelie Edrada-Ebel4, Safwat A. Ahmed3
1Department of Botany II, Julius-von-Sachs Institute for Biological Sciences, University of Würzburg, Würzburg, Germany 2Department of Microbiology, Biocenter, University of Würzburg, Würzburg, Germany 3Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt 4Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK 5Permanent address: Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt
Abstract Marine sponges are rich sources of natural products exhibiting diverse biological activities. Bioactivity-guided fractionation of the Red Sea spongeCallyspongia aff. implexa led to the isolation of two new compounds, 26,27-bisnorcholest-5,16-dien-23-yn-
3β,7α-diol, gelliusterol E (1) and C27-polyacetylene, callimplexen A (2), in addition to the known compound β-sitosterol (3). The structures of the isolated compounds were determined by 1D- and 2D-NMR techniques as well as high-resolution tandem mass spectrometry and by comparison to the literature. The three compounds (1–3) were tested against Chlamydia trachomatis, an obligate intracellular gram-negative bacterium, which is the leading cause of ocular and genital infections worldwide. Only gelliusterol E (1) inhibited the formation and growth of chlamydial inclusions in a dose-dependent manner with an IC50 value of 2.3 µM.
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Published Research Articles in International Journals 2014-2015
Phytochemistry 2015 (117): 194-199
Minor oxygenated cannabinoids from high potency Cannabis sativa L.
Safwat A.Ahmed Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
Abstract
Nine oxygenated cannabinoids were isolated from a high potency Cannabis sativa L. variety. Structure elucidation was achieved using spectroscopic techniques, including 1D and 2D NMR, HRMS and GC–MS. These minor compounds include four hexahydrocannabinols, four tetrahydrocannabinols, and one hydroxylated cannabinol, namely 9α-hydroxyhexahydrocannabinol, 7-oxo-9α-hydroxyhexa-hydrocannabinol, 10α- hydroxyhexahydrocannabinol, 10aR-hydroxyhexahydrocannabinol, Δ9-THC aldehyde A, 8-oxo-Δ9-THC, 10aα-hydroxy-10-oxo-Δ8-THC, 9α-hydroxy-10-oxo-Δ6a,10a-THC, and 1′S- hydroxycannabinol, respectively. The latter compound showed moderate anti-MRSa
(IC50 10.0 μg/mL), moderate antileishmanial (IC50 14.0 μg/mL) and mild antimalarial activity against Plasmodium falciparum (D6 clone) and P. falciparum (W2 clone) with IC50 values of 3.4 and 2.3 μg/mL, respectively.
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Published Research Articles in International Journals 2014-2015
Behavioural Pharmacology 2015 (4): 369–382
Fluvoxamine alleviates seizure activity and downregulates hippocampal GAP-43 expression in pentylenetetrazole-kindled mice: role of 5-
HT3 receptors.
Zaitone, Sawsan A.; Moustafa, Yasser M.
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
Abstract
Epilepsy has been documented to lead to many changes in the nervous system including cell loss and mossy fiber sprouting. Neuronal loss and aberrant neuroplastic changes in the dentate gyrus of the hippocampus have been identified in the pentylenetetrazole (PTZ) kindling model. Antiseizure activity of selective serotonin reuptake inhibitors has been reported in several studies. In the current study, the protective effect of fluvoxamine against PTZ-kindling was investigated in terms of seizure scores, neuronal loss, and regulation of hippocampal neuroplasticity. Further, the role of 5-HT3 receptors was determined. Kindling was induced by repeated injections of PTZ (35 mg/kg) thrice weekly, for a total of 13 injections. One hundred male albino mice were allocated into 10 groups: (1) saline, (2) PTZ, (3) diazepam (1 mg/kg)+PTZ, (4–6) fluvoxamine (5, 10 or 20 mg/kg)+PTZ, (7) ondansetron+fluvoxamine (20 mg/kg)+PTZ, (8) ondansetron+PTZ group, (9) ondansetron (2 mg/kg, i.p.)+saline, and (10) fluvoxamine (20 mg/kg)+saline. PTZ- kindled mice showed high seizure activity, hippocampal neuronal loss, and expression of growth-associated phosphoprotein (GAP-43) compared with saline-treated mice. Repeated administration of fluvoxamine (20 mg/kg) in PTZ-kindled mice suppressed seizure scores, protected against hippocampal neuronal loss, and downregulated GAP-43 expression, without producing any signs of the 5-HT syndrome in healthy rats.
Importantly, pretreatment with a selective 5-HT3receptor blocker (ondansetron) attenuated the aforementioned effects of fluvoxamine. In conclusion, the ameliorating effect of fluvoxamine on hippocampal neurons and neuroplasticity in PTZ-kindled mice was, at least in part, dependent on enhancement of hippocampal serotoninergic transmission at 5-HT3 receptors.
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Published Research Articles in International Journals 2014-2015
Journal of Apicultural Research 2015 (54): 121-122
Presence and infestation rate of Senotainia tricuspis (Meigen) (Diptera, Sarcophagidae) on honey bees in the Mediterranean Region.
Mohamed A. Shebld
dFaculty of Agriculture, Department of Plant Protection, Suez Canal University, Ismailia, Egypt.
Abstract
Our investigations show that the S. tricuspis is found in four countries out of the seven sampled ones. This pest was recorded in Egypt, Jordan, Algeria, and Italy, Samples from Iraq, Lebanon and Palestine were negative for infestation. We confirmed the presence of S. tricuspis in the southern and western areas of the Mediterranean sea, and diagnosed its existence for the first time in Egypt, but the big variation in the infestation level comparing the northern countries of the sea to the southern countries, propose two hypotheses: the first one is that this parasite prefers the wet areas rather than dray ones. The second hypothesis is that Apis mellifera ligustica bees are more susceptible than the bees of the southern and eastern countries of the Mediterranean region. To confirm such hypotheses further investigations in the region need to be conducted by collecting bigger sample size from the region. We also propose that further DNA studies are needed in order to build a phylogenic table to understand the genetic diversity and population genetics of S. tricuspis in the region. A wide Mediterranean North -South collaboration in technology transfer, extension and awareness program will be very helpful for the beekeeper sector in this region.
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Published Research Articles in International Journals 2014-2015
Postharvest Biology and Technology 2015 (100): 205-211
Storability, shelf-life and quality assurance of sugar snap peas (cv. super sugar snap) using modified atmosphere packaging
Mohammed W.M.Elwana..Ibrahim N.Nasefa.Samir K.El-Seifia..Mahmoud A.Hassana. Rawia E.Ibrahimb. a Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt bVegetable Handling Research Department, Agriculture Research Center, Giza, Egypt
Abstract This investigation was aimed at selecting the most suitable package to maintain quality of sugar snap peas pods. The effectiveness of five types of polypropylene packages: highly perforated (HPPP), non-perforated (NPPP) and micro-perforated with 6, 12 and 24 holes (MPPP6, MPPP12 and MPPP24) on storability of pods was studied during cold storage at 0 °C with 90–95% RH for 7, 14, 21 days and simulating shelf-life conditions at10 °C with 80–85% RH for 2 or 4 days after 21 days at 0 °C. O2 and CO2concentrations, weight loss, visual quality, off odors, decay, color, firmness, crispness, taste, total chlorophyll, vitamin C, SSC, and total sugar contents were measured. Results revealed that O2 decreased and CO2 increased slowly inside MPPP6, MPPP12 and MPPP24 bags, however, the reduction in O2 and the increments in CO2 in NPPP bags were very sharp and accompanied with high levels of off odors. HPPP had the highest weight loss compared with other bags. MPPP12 bags maintained quality during storage and simulated shelf-life, in terms of higher scores for visual quality, firmness, crispness and taste as well as highest contents of chlorophyll, vitamin C and sugars. NPPP bags had the worst values for quality. At the end of storage and shelf-life, an increment in h* was observed in samples stored in MPPP6, MPPP12 and MPPP24 bags (more green color) in comparison with those in NPPP bags.
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Published Research Articles in International Journals 2014-2015
Food Chemistry 2015 (176): 99–105
Compositional analysis of genetically modified corn events (NK603, MON88017 × MON810 and MON89034 × MON88017) compared to conventional corn.
Ahmed M. Rayan a
a Food Technology Department, Faculty of Agriculture, Suez Canal University, Ismailia 41522, Egypt
Abstract
Compositional analysis of genetically modified (GM) crops continues to be an important part of the overall evaluation in the safety assessment for these materials. The present study was designed to detect the genetic modifications and investigate the compositional analysis of GM corn containing traits of multiple genes (NK603, MON88017 × MON810 and MON89034 × MON88017) compared with non-GM corn. Values for most biochemical components assessed for the GM corn samples were similar to those of the non-GM control or were within the literature range. Significant increases were observed in protein, fat, fiber and fatty acids of the GM corn samples. The observed increases may be due to the synergistic effect of new traits introduced into corn varieties. Furthermore, SDS–PAGE analysis showed high similarity among the protein fractions of the investigated corn samples. These data indicate that GM corn samples were compositionally equivalent to, and as nutritious as, non-GM corn.
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Published Research Articles in International Journals 2014-2015
Applied Entomology and Zoology 2015 (50): 175-182
Tomato treatment with chemical inducers reduces the performance of Spodoptera littoralis (Lepidoptera: Noctuidae).
Islam S. Sobhy, Nasser S. Mandour, Awad A. Sarhan Department of Plant Protection, Faculty of Agriculture, Public Service Center of Biological Control (PSCBC), Suez Canal University, Ismailia, Egypt.
Abstract The evolving understanding of plant signaling pathways has promoted the possibility of using chemical inducers as an effective tactic for crop protection. In this study, under greenhouse conditions, we conducted a growth assay of Spodoptera littoralis (Boisduval) larvae on tomato plants treated with BTH (S-methyl benzo [1, 2, 3] thiadiazole 7 carbothioate) as a salicylic acid mimic, PDJ (propyl [1RS, 2RS]-[3-oxo-2- pentylcyclopentyl] acetate) as a jasmonic acid-mimic or both chemicals as a combined treatment. The larval body weight of S. littoralis was drastically reduced with each chemical compared to control plants, and there was a significant synergistic interaction. Overall, the total weight gain of surviving larvae fed on treated plants was distinctly tenfold less than for those fed on control plants. Moreover, incorporating the chemical inducers in artificial diets had no direct or toxic impact on the larval body weight of S. littoralis under laboratory conditions. Larval survival rates were significantly lower (35–40 %) on treated plants with either combined or independent inducers‟ treatments compared with control plants after 15-day feeding. In contrast, incorporating the chemical inducers in artificial diets had no direct effect on larval survival rates under laboratory conditions. The applied concentrations of BTH and PDJ had no detectable phytotoxicity to tomato plants. Our results demonstrate that BTH and PDJ can act synergistically when applied to tomato to reduce the performance of S. littoralis. These findings stress that the application of chemical inducers could provide an environment-friendly tactic to help manage insect pests and thereby play multiple roles in improving the overall plant resistance to herbivore pests.
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Published Research Articles in International Journals 2014-2015
Transactions of the ASABE 2015 (3): 849-859
Development of a multi-scale model for deep-bed drying of rice
Ramadan ElGamal, Frederik Ronsse, Gamal ElMasry, Jan G. Pieters
Department of Agricultural Engineering, Suez Canal University, Ismailia, Egypt.
Abstract
The drying behavior of rough rice in a deep bed was analyzed numerically by solving the heat and moisture transfer equations using a novel deep-bed model. The model consisted of two scales. A computational fluid dynamics (CFD) model was used first to predict the convective coefficients of heat and mass transfer between an individual rough rice kernel and air in the rough rice bed. The predicted heat and mass transfer coefficients were then used for coupling the moisture and heat fluxes inside the rough rice kernel with the external convective heat and mass transfers at the kernels‟ surfaces in a complete rough rice bed model using the Comsol Multiphysics simulation environment. The developed model was used to predict the grain moisture contents and temperatures at different heights in the bed during drying. The theoretical predictions of moisture profiles inside a deep bed of rough rice were validated against experimental data from literature. The results revealed that the mean relative deviation between predicted and experimental values varied between 3.1% and 6.8%. Most importantly, the novel approach used in this study allowed the deep-bed model to predict the moisture and temperature distributions inside the individual rough rice kernels at different heights in the rough rice bed.
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Published Research Articles in International Journals 2014-2015
Euphytica 2015 (201): 389-400
Evaluation of cauliflower genebank accessions under organic and conventional cultivation in Southern Germany.
Eltohamy A. Yousef, Christian Lampei and Karl J. Schmid
Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt.
Abstract In recent years, public attention increased towards products from organic farming due to their presumed higher quality and health benefits. Frequently, organic farming is characterized by lower yields than conventional farming. One reason may be the use of varieties that were bred for conventional cultivation and are not adapted to organic farming. This raises the question if high yielding varieties differ in their performance under different cultivation methods allowing the selection of varieties with superior performance in organic cultivation. To answer this question and to identify suitable genotypes we evaluated a collection of 178 cauliflower genebank accessions under organic and conventional farming conditions. Two traits (curd width and time to budding) were evaluated for mean and stability. We observed a significant genotype × cultivation method interaction because genotypes differed in their performance between cultivation methods. Of the two traits investigated, curd width showed a lower heritability (H2org= 0.26, H²conv= 0.37) and low genotypic correlation between organic and conventional systems, compared to days to budding that show high heritability (H²org= 0.86, H²conv= 0.87) and a high correlation between the two farming systems. Our results demonstrate that the selection for curd width should be preferably conducted under organic conditions, whereas selection for number of days can be carried out under organic or conventional conditions. The evaluation of genotypes at both environments identified genotypes that may be used as parental lines for breeding under organic conditions.
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Published Research Articles in International Journals 2014-2015
Plant Protect. Sci. 2015 51: 39–45
Laboratory evaluation of the entomopathogenic fungus, Hirsutella thompsonii and Paecilomyces fumosoroseus against the citrus brown mite, Eutetranychus orientalis (Acari: Tetranychidae).
Hamdy Mahmoud El-Sharabasy
Plant Protection Department, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt.
Abstract
The citrus brown mite, Eutetranychus orientalis (Klein) is a serious pest attackscitrus in Egypt. Formulations of the entomopathogenic fungi Hirsutella thompsonii and Paecilomyces fumosoroseus were tested against all stages of this pest under laboratory conditions. Three concentrations: 0.5×109, 1.0×109 and 2.0×109 conidia/ml were sprayed on leaf discs containing, larvae, nymphs and adults, while a single dosage 1.0×109 wassprayed on eggs as ovicides. All the life stages were susceptible to both fungal pathogens at the tested concentrations. Larval and nymphal stages were generally less susceptible than adults. Based on probit analysis, H.thompsonii was the highest virulent with its LC50 and LT50 (358, 290 and 146 conidia/ml and 7.78, 7.11 and 6.92 days) from P. fumosoroseus(597, 589 and 339 conidia/ml and 7.49, 7.14 and 4.31 days) for larvae, nymphs and adults, respectively. However the two pathogens were evaluated proved their superiority over water spraying and untreated control treatments. Egg hatchability was reduced at the different concentrations compared with controls. H. thompsonii with its
LC50 apparently more infectious to the mite eggs. Also, females in the control laid more eggs than those of fungus-treated. The entomopathogenic fungi H. thompsonii and P. fumosoroseus could be considered as an environmentally friendly alternative for biocontrol of E. orientalis.
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Published Research Articles in International Journals 2014-2015
Egyptian Journal of Biological Pest Control 2015 51: 39–45
Efficacy of two phytoseiid predators and a biopesticide against Tetranychus cucurbitacearum (Sayed) (Acari: Tetranychidae) on Eggplant at Ismailia Governorate, Egypt.
El-Saiedy,E.M.; M.F. Hassan; A.F. El Bahrawy; G.A. El Kady and Marwa S.Kamel
Plant Protection Dept. National Research Center, Giza, Egypt. Zoology and Agricultural Nematology Dept. Faculty of Agriculture, Cairo University, Giza, Egypt. Plant Protection Dept. Faculty of Agriculture, Suez Canal University, Ismailia, Egypt.
Abstract Efficacy of the two predatory mites species; Amblyseius swirskii Athias-Henriot, Neoseiulis californicus (McGregor) and the biopesticide Abamectin 50% EC against the Tetranycid mite, Tetranychus cucurbitacearum (Sayed) when infested six eggplant cultivars (White, Black stream beity, Baladi, Black King, Roma and Taska) was evaiuated under open field and greenhouse conditions at Ismailia Governorate, Egypt in two plantation (Summer and Nili of year 2012). Highly reduction percentage was achieved by the predatory mite N. californicus, followed by the biopesticide (Abamectin) and the leastone was given by A. swirskii. Release of N.californicus and application of Abamectin showed a combination of a successful control management strategy for T.cucurbitacecrum on eggplant.
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Published Research Articles in International Journals 2014-2015
Egyptian Journal of Biological Pest Control 2015 2: 355-358
Evaluation of Entomopathogenic Nematode, Steirnernema carpocapsae against the German Cockroach, Blattella germanica(L.) under Laboratory Conditions.
El-Kady, G. A.; Y. S. El-Badry; A. F. El-Bahrawy; H. M. El-Sharabasy and M. F.Mahmoud
Plant Protection Dept., Faculty of Agriculture, Suez Canal University, 41522 Ismailia, Egypt.
Abstract
Efficacy of the two predatory mites species; Amblyseius swirskii Athias-Henriot, Neoseiulis californicus (McGregor) and the biopesticide Abamectin 50% EC against the Tetranycid mite, Tetranychus cucurbitacearum (Sayed) when infested six eggplant cultivars (White, Black stream beity, Baladi, Black King, Roma and Taska) was evaiuated under open field and greenhouse conditions at Ismailia Governorate, Egypt in two plantation (Summer and Nili of year 2012). Highly reduction percentage was achieved by the predatory mite N. californicus, followed by the biopesticide (Abamectin) and the leastone was given by A. swirskii. Release of N.californicus and application of Abamectin showed a combination of a successful control management strategy for T.cucurbitacecrum on eggplant.
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Published Research Articles in International Journals 2014-2015
Biocontrol Science and Technology 2015 5, 525–542
Resistance induction in pumpkin Cucurbita maxima L. againstWatermelon mosaic potyvirus by plant growth-promoting rhizobacteria
Essam. K.F. Elbeshehya,b, Sahar A. Youssefb and Ahmed M. Elazzazya,c*
Department of Biological Sciences, Faculty of Science (North Jeddah), King Abdul Aziz. University, Jeddah, Saudi Arabia; bDepartment of Botany, Faculty of Agriculture, Suez Canal University, Ismaïlia, Egypt; cDepartment of Chemistry of Natural and Microbial Products, National Research Centre, Division of Pharmaceutical Industries, Giza, Egypt.
Abstract
Watermelon mosaic potyvirus (WMV) is a serious threat to pumpkin production inEgypt. To date, few reports have examined the potential of plant growthpromotingrhizobacteria (PGPR) to induce systemic resistance against diseases ofseveral plant viruses. The aim of this study was to determine the potential ofselected PGPR strains on induction of host systemic resistance within pumpkinplants (Cucurbita maxima L.) against WMV infection. The WMV was isolatedfrom naturally infected pumpkin and squash plants in Jeddah, Saudi Arabia.Typical symptoms of WMV were observed after mechanical inoculation. Viral identification was confirmed by double antibody sandwich enzyme-linkedimmuno-sorbent assay and reverse transcription polymerase chain reaction.Two PGPR strains designated as Bacillus subtilis 281 (B1) strain and Bacilluspumilus 293 (B2) were tested individually and in combination for their effectivenessagainst the WMV infection. The results of greenhouse experiments withpumpkin plants were challenged by mechanical inoculation, and demonstratedthat separate or combined treatment with PGPR strains B1 and B2, applied at 1 ×108 colony-forming unit/ml, significantly (P < 0.05) enhanced the plant height andfresh weight, while clearly lowering the disease severity rating and virusconcentrations, in all repeated greenhouse trials compared to the non-treatedcontrol. Seedlings treated with B2 strain demonstrated significantly lower levels ofdisease than B1 + B2 or B1, respectively, in all trials compared to control. Ourfindings demonstrated the potential of selected PGPR strains to induce systemicresistance for WMV infection.
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Egyptian Journal of Biological Pest Control 2015 1, 113-120
Evaluation of Two Protective Strategies for Controlling the Tomato Leaf miner Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) in Egypt.
Mohamed A. M. Osman Plant Protection Department, Faculty of Agriculture, Suez Canal University 41522, Ismailia, Egypt
Abstract
The tomato leafminer (TLM), Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), is an invasive pest to Egypt since 2010. It has become a serious threat to tomato crop either in open fields or in greenhouses at Ismailia Governorate and elsewhere in Egypt. Efficacy of two different control strategies; bio-rational insecticides and sex pheromone mass trapping was evaluated. Six bio-rational insecticides; Avaunt, Coragen, Demerone, Proclaim, Radient and Runner at their applied rates were evaluated against TLM under laboratory and field conditions. Two kinds of sex pheromone dispensers (Tutacap and Pherodis) with two types of traps (Delta trap and Water-pane trap) were also evaluated under field conditions. Results indicated that the tested insecticides showed various toxicity to egg hatchability, second and third instar larvae of TLM. Radient, Proclaim and Coragen achieved highest accumulated mortalities in TLM larvae (100%), 5 days post treatment. Demerone and Runner showed moderate toxicity toward second and third instars. Under field conditions, the same trend of effectiveness was also observed being highest in Proclaim, followed by Radient, Coragen and Avaunt. Results of mass trapping revealed that the attractant response of TLM males to pheromone-baited traps was affected by the weather conditions, especially ambient temperature. Tutacap dispenser with water-pane traps was more efficient than Pherodis dispenser with delta or water-pane traps. Mass trapping as a control strategy against TLM was effective in reducing the level of infestation, to a much lesser extent than the use of bio-rational insecticides.
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Published Research Articles in International Journals 2014-2015
Talanta 2015 143, 145–156
Freshnessestimationofintactfrozen fish using fluorescencespectro- scopyandchemometricsofexcitation–emission matrix
Gamal ElMasry a,b,n, HirotoNagai a, KeisukeMoria c, NahoNakazawa c, MizukiTsuta d, Junichi Sugiyama d, EmikoOkazaki c, ShigekiNakauchi
a Department of Computer Science and Engineering, Toyohashi University of Technology, Toyohashi, Aichi, Japan b Suez Canal University, Faculty of Agriculture, Agricultural Engineering Department, Ismailia, Egypt c Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, Japan d Food Engineering Division, National Food Research Institute, National Agriculture and Food Research Organization, Tskuba, Japan
Abstract The current study attempted to provide a convenient, non-invasive and time-saving method to estimate the freshness of intact horse mackerel (Trachurus japonicus) fish in a frozen state using autofluorescence spectroscopy in tandem with multivariate analysis of fluorescence excitation–emission matrices (EEM). The extracted fluorescence data from different freshness conditions were pretreated, masked and reorganized to resolve fish fluorescence spectra from overlapping signals and scattering profiles for detecting and characterizing freshness changes. The real freshness values of the examined fish samples were then traditionally determined by the hard chemical analysis using the high performance liquid chromatography (HPLC) method and expressed as K-values. The fluorescence EEM data and the real freshness values were modeled using partial least square (PLS) regression and a novel algorithm was proposed to identify the ideal combinations of excitation and emission wavelengths being used as perfect predictors. The results revealed that freshness of frozen fish could be accurately predicted with R2 of 0.89 and root mean square error estimated by cross validation (RMSECV) of 9.66%. This work substantially demonstrated that the autofluorescence spectroscopy associated with the proposed technical approaches has a high potential in non-destructive sensing of fish freshness in the frozen state.
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ANNALS OF MICROBIOLOGY
2015
Evaluation of the extent of spreading of virulence factors and antibiotic resistance in Enterococci isolated from fermented and unfermented foods
Mohamed Abouelnaga2& Alexandre Lamas1 & Marcos Quintela-Baluja1 &Magdy Osman2& José Manuel Miranda1 & Alberto Cepeda1 & Carlos Manuel Franco1
1 Department of Analytical Chemistry, Nutrition and Food Science,School of Veterinary Sciences, University of Santiago deCompostela, Campus Universitario, 27002 Lugo, Spain 2 Dairy Department, Faculty of Agriculture, Suez Canal University,41522 Ismailia, Egypt
Abstract The aim of this work was to evaluate the impact of fermentation processes on the incidence of virulence and antibiotic resistance determinants in Enterococcus strains from food origin (milk, fermented milk, cheese, fresh meat and fermented meat) with regards to potential pathogenicity. A total of 167 enterococcal strains were used in this study. Of those, 2 were reference strains, and 165 were isolated and identified by molecular methods and screened for virulence factors including ace, agg, ccf, cpd, esp, efaAfm and gelE as well as resistance against ten antibiotics. Enterococcal isolates were identified as Enterococcus faecium (54), Enterococcus faecalis (86), Enterococcus casseliflavus (10) and other Enterococcus spp. (15). Only 3 out of 88 fermented food isolates were free from virulence determinants while approximately 16 % of strains isolated from unfermented foods were free from virulence determinants. Unusually, the Ace gene was detected in E. faecium, especially in fermented foods. In contrast, antibiotic resistance in enterococci was not potentiated by the fermentation process. This study has revealed the important role that may be played by fermentation processes in virulence gene incidence and the potential of such processes to disseminate these traits throughout the enterococci food chain.
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Published Research Articles in International Journals 2014-2015
Chemosphere 2015 (120) 457–461
Residues of organochlorine and synthetic pyrethroid pesticides in honey,an indicator of ambient environment, a pilot study
Farag M. Malhat a,⇑, Mohamed N. Haggag a, Naglaa M. Loutfy b, Mohamed A.M. Osman b,Mohamed Tawfic Ahmed b,⇑ a Pesticide Residues and Environmental Pollution Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center, Dokki, Giza 12618, Egypt b Faculty of Agriculture, Suez Canal University, Ismailia 41522, Egypt
Abstract
Study meant to examine the quality of honey, and to use honey as a bioindicator of environmentalcontamination. Residue levels were determined by gas chromatography (GC-lECD). Samples had a widespectrum of organochlorine and synthetic pyrethroids pesticides, with hexachlorobenzene (HCB) asthe most frequently detected organochlorine, followed by permethrin, heptachlor epoxide. Only onesample had a concentration of c- HCH higher than maximum residue limit of honey (0.01 mg kg_1). Residues of organochlorines detected, indicate the presence of some fresh supplies, despite the banimposed on their use. The study confirmed that honey bee and beehive matrices could be used as gaugefor monitoring environment contamination. From public health point of view, the observed levels ofpesticide residues in honey do not pose a serious health risk to the consumers, but raises questions ofthe source of organochlorines.
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Published Research Articles in International Journals 2014-2015
Regulatory Toxicology and Pharmacology 2015 1-7
Hazard index, a tool for a long term risk assessment of pesticide residues in some commodities, a pilot study
Sohair A. Gad Alla a, Naglaa M. Loutfy b, Amr H. Shendy a, Mohamed Tawfic Ahmed b, * a Central Laboratory of Residue Analysis of Pesticides and Heavy Metals in Food (QCAP), Agricultural Research Center, Giza, Egypt b Faculty of Agriculture, Suez Canal University, Ismailia 41522, Egypt
Abstract
Samples of some vegetables were analyzed for pesticides residues using the accredited (QuEChERS)method. The method allowed the determination of 215 compounds of different pesticide chemicalgroups. LC-MS/MS and GCeMS/MS were used for residues quantification. In a total number of 116samples, no pesticides residues were detected in 34 samples (29.3%), while 82 samples (70.7%) haddetectable pesticide residues, with some samples exceeding the MRLs levels established by the CodexAlimentarius Commission. The hazard index (HI %), representing the long e term risk assessment was inthe range of 0.01%e15.04% of the ADI's. The highest exposure was observed for ethion, followed bychlorpyifos, both of them are organophosphates, at15.04% and 2.45% of ADI respectively. The acute(short-term) exposure was also estimated. Results showed a potential risk for children posed by 3pesticides, meanwhile, residues of one pesticides showed potential risk to adults (>100% of ARfD). Thepresent work is an attempt to provide a model for the use of WHO template for calculating the short termintake. This model is especially useful for developing countries where information about consumptionrate is rather meager.
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Published Research Articles in International Journals 2014-2015
Applied Mathematics and Computation 2015 (251) 169–179
On Bertrand duopoly game with differentiated goods
E. Ahmed 1 , A.A. Elsadany 2,3 , Tonu Puu 4
1. Department of Mathematics, Faculty of Science, Mansoura University, Egypt 2. Department of Basic Science, Faculty of Computers and Informatics, Ismailia 41522, Suez Canal University, Egypt 3.Department of Mathematics, Shanghai University, Shanghai 200444, China 4. Umea University, Faculty of Social Sciences, Centre for Regional Science (CERUM), 90187 Umea, Sweden
Abstract
The paper investigates a dynamic Bertrand duopoly with differentiated goods in which boundedly rational firms apply a gradient adjustment mechanism to update their price in each period. The demand functions are derived from an underlying CES utility function. We investigate numerically the dynamical properties of the model. We consider two specific parameterizations for the CES function and study the Nash equilibrium and its local stability in the models. The general finding is that the Nash equilibrium becomes unstable as the speed of adjustment increases. The Nash equilibrium loses stability through a period-doubling bifurcation and the system eventually becomes chaotic either through a series of period-doubling bifurcations or after a Neimark–Sacker bifurcation.
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Published Research Articles in International Journals 2014-2015
Applied Soft Computing 2015 (26) 418–427
An edge detection scheme based on least squares support vectormachine in a contourlet HMT domain.
Gh.S. El-tawela, ∗, A.K. Helmyb aComputer Science, Faculty of Computers and Informatics, Suez Canal University, EgyptbNational Authority of Remote Sensing and Space Sciences, Cairo, Egypt
Abstract tIn this paper, we have presented a new and effective edge detection scheme based on least squaressupport vector machine (LS-SVM) classification in a contourlet Hidden Markov Tree Model (contourletHMT). First, the input noisy image is decomposed into coarser and finer coefficients using a contourletHMT transform to derive an efficient multiscale and multidirectional image representation. Second, thefeature vector is performed through spatial regularity in a contourlet HMT domain, and the coarsercoefficients classified using LS-SVM classifier into two classes: noise coefficients and edge coefficients.Next, all noisy contourlet HMT coefficients are well denoised by the BayesShrink method.Finally, the denoised coefficients and edge coefficients are fused using the weighted average rule, andthe inverse contourlet HMT is applied to obtain the edge image.Experimental results demonstrate that our scheme can attain improved performance over state-of-the-art edge detection approaches, both qualitatively and quantitatively. Tests were performed on severalimages from the Berkeley dataset corrupted with Gaussian noise and on other images such as a camera-man, pepper and medical images. The results illustrate that the performance of the proposed scheme isstable.
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Published Research Articles in International Journals 2014-2015
Wulfenia Journal 2015
On study of MHD flow of a viscous fluid on nonlinear porous shrinking sheet. A. M. Salem a , M. M. Allan b) and Samah M Darderyb) a Department of Mathematics, Faculty of computer and information, Suez Canal University, Egypt. b Department of Mathematics, Faculty of Science, Zagazig University, Egypt
Abstract
The present article aims to investigate the analytical and numerical solution of a magnetohydrodynamic (MHD) flow of a viscous fluid fluid towards a nonlinear porous shrinking sheet. The analytical solution of this problem is computed by the Adomian decomposition method (ADM). The ADM is provided an analytical solution in the form of an infinite power series. The governing equation of the MHD flow is simplified to boundary value problem (BVP) by similarity transformations and solved numerically using the Rung- Kutta method with the shooting technique. The pertinent parameters appearing in the problem are discussed graphically and presented in tables. Convergent [m,m] Pad‟e approximations are obtained and compared with the numerical results. It is found that the Pad‟e approximants agree well with the numerical results.
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Progress in Nuclear Energy 2015 (73)11-20
Liapunov's stability on autonomous nuclear reactor dynamical systems
Yasser MohamedHamada Department of Basic Science, Faculty of Computers and Informatics, Suez Canal University, 41522 Ismailia, Egypt
Abstract Point kinetics model for nuclear reactor physics leads to a stiff system of ordinary differential equations. Stability conditions for linear and nonlinear nuclear reactor point kinetics equations are determined without explicitly solving these systems. Three different techniques, linearization, Liapunov's direct, and variable gradient methods, are applied to investigate the stability of the equilibrium solutions of the proposed systems. Linear system is solved analytically to check the results obtained by Liapunov's direct and variable gradient methods. For the autonomous nonlinear system with Newtonian feedback, linearization technique is used to discuss the stability in a small vicinity of the equilibrium solution, while variable gradient method is applied to establish asymptotic stability. The nonlinear system is close to the linear system, so that it has been proved to be almost linear. Generalized power series method (GPWS) with variable step sizes is used as a numerical method to solve the nonlinear system. Results of numerical calculations are presented in the form of diagrams that confirm the validity of stability conditions obtained by Liapunov's methods. Our results indicate that no trajectories go to infinity as the time tends to infinity. Also, it is proved that the system is conservative by constructing a Liapunov's function, which is valid everywhere without any restrictions, associated with zero Liapunov's derivative function.
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Solar Energy 2015 (122)900–909
Thermal analysis of a conical solar still performance: An experimentalstudy
H.E. Gad a, Sh. Shams El-Din b, A.A. Hussien b,⇑, Kh. Ramzy c a Department of Mechanical Power Engineering, Faculty of Engineering, Mansoura University, Egypt b Department of Mechanical Power Engineering, Faculty of Engineering, Menoufiya University, Egypt c Department of Mechanical Engineering, Faculty of Engineering, Suez-Canal University, Egypt
Abstract In this paper, an attempt is made to estimate the heat transfer coefficients of a conical solar still. Many researches and developmentworks tried to enhance the productivity of solar stills using different methods. So in this study the productivity enhancement of solar stillby decreasing the shadow effect and maximize utilization of solar radiation is discussed. A conical solar still was designed and manufacturedat faculty of engineering Sheben El-Kom – Egypt (latitude 30.56 N and longitude 31.01 E). The still base area was 0.8 m2, and theacrylic cover of still inclined at 31_ which equal to the city latitude. The experimental results of conical solar still were compared with aconventional type solar still which has the same area. The results showed that, the daily productivity for conical and conventional solarstills was 3.38 and 1.93 L/m2 day, respectively. Heat and mass transfer coefficients were evaluated and the Nusselt and Sherwood numberswere calculated with the aid of both evaporation measurements and Chilton–Colburn analogy. The maximum value of the total heattransfer coefficient were 66 and 32 W/m2 _C for conical and conventional solar stills, respectively. The analogy between heat and masstransfer coefficients was also investigated.
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Renewable Energy
2015
(78)484-497
Numerical and experimental studies on updraft gasifier HTAG
T.M. Ismail a, *, M. Abd El-Salam b a Mechanical Engineering Department, Suez Canal University, Ismailia, Egypt b Basic Science Department, Cairo University, Giza, Egypt
Abstract
In the present work, the performance of an updraft gasifier was investigated experimentally andnumerically. This study aimed to use a two-dimensional model using a code, namely COMMENT code, toinvestigate the gasification and combustion processes while taking into consideration the process ratewithin the gasifier. In the present model the kinetic theory of granular flow (KTGF) was introduced tosimulate the solid phase, while gas phase turbulence was modeled under ke 3 model. The predictedtemperature and gas composition profiles are in good agreement with the experimental work obtainedfrom the updraft gasifier at the Royal Institute of Technology (KTH). These results show that the presentmodel is a promising tool for simulating the gasification/combustion process of biomass within thegasifier.
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J Coast Conserv 2015 19:589–608
Implications of climate change on the groundwater flow regime and geochemistry of the Nile Delta, Egypt
Mohamed H. Geriesh1& Klaus-Dieter Balke2 &Ahmed E. El-Rayes1&Basma M. Mansour1
1Geology Department, Faculty of Science, Suez Canal University,Ismailia, Egypt 2 Faculty of Mathematic and Natural Sciences, Tuebingen University,Tuebingen, Germany
Abstract Environmental isotope analyses in conjunction with the hydro-geochemical investigations and tentative reviewing of the paleoclimatic sea level changes are carried out to fingerprint the implications of climatic changes on the groundwater flow regime and geochemistry at the Nile Delta. Following up the footprints of groundwater flow history, it is observed that the Pleistocene, main groundwater aquifer of the Nile Delta was drained and refilled with Nile water several times due to the eustatic sea level propagations between dry and wet periods. Therefore, the present-day groundwater flow regime could be affected by the latest Holocene phase of climate changes during which no significant dramatic sea level changes were recorded. After the time slice of the Mediterranean humid phase, 8000-5500 BP, the sea level started to rise steeply from –15 m to the present-day level. Under this rising rate, the seawater invaded most of the northern delta lope and several 10ths of kilometres inland via the mouths of the ancient Nile branches. During these arid conditions, deterioration of the Pleistocene unconfined aquifer could take place. This finding matches a famine and economic instabilities during the arid periods and flourishing and economic stabilities during the humid pluvial periods along the history of the ancient Egyptian civilization. In accordance to the latest active sea level rise stage in conjunction with the delta subsidence, a contagious groundwater level rise with a recent order of 3 cm/year is taking place leading to form several lake-like lagoons, water logging and soil salinization along the coastal plain and the eastern low lands. The Nile Delta is expected to suffer extreme soil salinization and gradual merging under the groundwater logging and seawater transgression especially, along the eastern coastal zone which suffers a high subsidence rate of about 5 mm/year. In contrast to previous studies, our findings show that, the present groundwater composition and salinity in the Nile Delta aquifers cannot be attributed to a recent seawater intrusion. The physico-chemical processes that explain this composition are combination of salt dissolution (mainly from Holocene fluviomarine aquifer), flushing by recent Nile water, ion exchange and evaporation.
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Arabian J. Geosciences 2015 8:5631-5647
Hydrogeochemical and seismological exploration for geothermal resources in South Sinai, Egypt utilizing GIS and remote sensing.
AHMED EL-RAYES,MOHAMED O. ARNOUS,HAMDY A. ABOULELA Faculty of Science, Suez Canal University, Ismailia, Egypt
Abstract
Geographic information system and remote sensing techniques are used to determine the spatial association between the hydrogeochemical and seismological evidences of well-known geothermal fields in South Sinai. Manipulation, combination, overlay, integration and reattribution GIS functions have been used to explore the possible new occurrences of geothermal resources in South Sinai. Moreover, surface observations and lineament analysis of satellite images are used to delineate the active geothermal areas. The distribution of recent earthquake epicenters over South Sinai shows three main active trends (N 6° E, N 40°–75° E and N 50°–60° W) conformable with the long fault extensions extracted from ETM+7 and ASTER GDEM satellite images and comprise the well-known hot springs in South Sinai. It is found that wherever the N 6° E trend intersects both of N 50°–60° W or N 40°–75° E, the temperature of spring waters is remarkably increased. This feature could be attributed to the deep penetration of groundwater along the N 6° E fault trend (Gulf of Aqaba trend). The seismicity patterns of well-known and newly predicted geothermal sites show that the majority of earthquakes have focal depth range between 3 and 33 km (within the upper Earth crust) and have micro to moderate magnitude (0.5≥Mb≤4.5). In addition, the maximum focal depth of events reached up to 50 km at the Hammam Faroun area revealing the deep-seated thermal source. The main chemical type of the known thermal water of South Sinai is Cl-Na, indicating a long flow pathway of this water. The high calcium contents of thermal water of South Sinai are accompanied with remarkably low sulphates. This could be attributed to the microbial or thermal reduction of sulphates in contact with organic carbon which is confirmed by the emission of H2S gas observed at the known thermal field sites. As a result, thermal waters of the Hammam Faroun and Hammam Musa are undersaturated with respect to gypsum and anhydrite. The hydrochemistry of the newly predicted thermal sites exhibit same chemical pattern similar to the known thermal waters. Such observations are used as indicators to locate six predicted new occurrences of geothermal fields in South Sinai
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Arabian Journal of Geosciences 2015 8:7899-7918
Heavy metals risk assessment in water and bottom sediments of the eastern part of Lake Manzala, Egypt, based on remote sensing and GIS
Mohamed O. Arnous* &Mohamed A. A. Hassan
*Department of Geology, Faculty of Science,Suez Canal University, Ismailia, Egypt Soil and Water Department, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt
Abstract The contamination of natural environment components such as soil, sediment, water and biota by heavy metals is a major, worldwide concern. The concentrations of Zn, Cd, Cu, Mn and Pb in water and bottom sediments of the Eastern Lake Manzala (ELM) are collected and analyzed. The geostatistical, change detection and land use/land cover analyses in addition to the geospatial mapping and GIS data integration are carried out in the present study to monitor and assess the different types of pollution. The findings indicated that the sediments and water of ELM constitute an ultimate sink for heavy metals in ELM aquatic system. During the last few years, the industrial together with the other anthropogenic activities has had a negative consequence for the natural environment of the ELM. This unfavourable condition also impacts the aquatic ecosystems. The contaminated water body of ELM with heavy metals greatly affected the lake food, organisms, and, hence, humans. The heavy metal concentration in ELM is found in riskier levels than the world permissible limits, which poses a serious environmental concern. The results revealed an agreement with abandoned heavy metal concentration effluents from human activities such as industrial, agricultural and domestic wastes in addition to sewage water and commercial activities. The ELM area is considered as a risky area contaminated with heavy metals, and it requires the establishment of awareness campaigns, periodic monitoring and evaluation along most communities of the lake margin. It is concluded that it is essential for planners and decision makers to seriously consider taking appropriate action now concerning the recommendations of the present study results establishing future sustainable development plan of the ELM.
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Published Research Articles in International Journals 2014-2015
Sensors and Actuators B: Chemical 2015 220:22–26
Humidity sensing properties of reduced humic acid El-shazly M. Duraiaa,b,∗, Gary W. Bealla,c aTexas State University-San Marcos, Department of Chemistry and Biochemistry, 601 University Dr., San Marcos, TX 78666, USA
bSuez Canal University, Faculty of Science, Physics Department, Ismailia, Egypt cFaculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
Abstract Humidity sensors are utilized as part of the control system in all air conditioning equipment in theworld. These important sensors can be divided into three main types including capacitive, resistance,and thermal conductivity. Of these the resistive type are the most cost effective, smallest in size, bestinterchangeability in the field, and long term stability. For the first time, we report the use of reducedhumic acid as a resistive humidity sensor (RH 12.4% up to 97.1%). This material is promising due to thelow cost, high sensitivity and environmentally friendliness. Moreover, this material has a fast responseand recovery time compared to the graphene oxide. In contrast to graphene oxide it was found that theresistance of the sensor decreases as the relative humidity increases. Humidity sensing mechanism ofthe reduced humic acid sensor has been discussed.
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Published Research Articles in International Journals 2014-2015
Spectrochimica Acta Part A: Molecular andBiomolecular
Spectroscopy 2015 220:22–26
Influence of homo buffer layer thickness on the quality of ZnO epilayers
E.A. Eid a, A.N. Fouda b,⇑ a Department of Basic Science, Higher Technological Institute, 44629 10th of Ramadan City, Egypt b Physics Department, Faculty of Science, Suez Canal University, 41522 Ismailia, Egypt
Abstract
ZnO buffer layers with different thicknesses were deposited on a-plane sapphire substrates at 300 _C. ZnOepilayers were grown on ZnO buffers at 600 _C by radio- frequency magnetron sputtering and vacuumannealed at 900 _C for an hour. Influence of nucleation layer thickness on the structural and quality ofZnO thin films was investigated using X-ray diffraction (XRD), atomic force microscopy (AFM), andRaman spectroscopy. The best ZnO film quality was obtained with the ZnO buffer layer of 45 nm thickwhich provided the smoothest surface with RMS value of 0.3 nm. X-ray diffraction measurements revealthat the films have a single phase wurtzite structure with (0001) preferred crystal orientation. As evidentfrom narrow FWHM of ZnO (0002) rocking curve, ZnO buffer can serve as a good template for the growthof high-quality ZnO films with little tilt. In addition, the micro-Raman scattering measurements at roomtemperature revealed the existence of Raman active phonon modes of ZnO; A1(TO), A1(LO) and E2(high). The latter two modes were not observed in thin buffer layer beside the dis-appearance of E2(low) modein all films.
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Published Research Articles in International Journals 2014-2015
Solar Energy Materials and Solar Cells 2015 133:69–75
Ferroelectric Bi3.25La0.75Ti3O12 photodiode for solar cell applications
. F.Yakuphanoglua,c.A.Tataroğlub..Ahmed A.Al-Ghamdic..R.K.Guptad.YusufAl- Turkie.Z.Şerbetçif. Saad BinOmrang.FaridEl-Tantawyh. aDepartment of Physics, Faculty of Science, Fırat University, Elazığ, Turkey bDepartment of Physics, Faculty of Science, Gazi University, Ankara, Turkey cDepartment of Physics, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia dDepartment of Chemistry, Pittsburg State University, Pittsburg, KS 66762, USA eDepartment of Electrical and Computer Engineering, Faculty of Engineering, King Abdulaziz University, Jeddah, Saudi Arabia fDepartment of Chemistry, Faculty of Arts and Sciences, Bingol University, Bingol, Turkey gDepartment of Physics and Astronomy, College of Science, King Saud University, Riyadh, Saudi Arabia hDepartment of Physics, Faculty of Science, Suez Canal University, Ismailia, Egypt
Abstract
Bi3.25La0.75Ti3O12 thin film was prepared on p-type silicon substrate by sol-gel spin coating method. The film indicates a ferroelectric behavior with a remanent polarization of ~18.2 μC/cm2. Electronic parameters such as ideality factor and barrier height of the diode were calculated to be 3.80 and 0.75 eV, respectively. The photocurrent results in the reverse bias of the diode indicate that photocurrent under illumination is higher than the dark current. The diode exhibited a substantial visible-light photovoltaic effect. The capacitance-voltage and conductance-voltage measurements were carried out in the frequency range of 10 kHz–1 MHz. The observed decrease in the capacitance and increase in the conductance with the increasing frequency were explained on the basis of interface states. The interface states density of the structure was determined using Hill- Coleman method and observed to decrease with increasing frequency. The obtained results indicate that ferroelectric Bi3.25La0.75Ti3O12/p-type silicon junction is a new class of photoconductive devices with low-cost fabrication and high photoresponsivity.
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Published Research Articles in International Journals 2014-2015
Synthetic Metals 2015 210:288-296
Logarithmic organic photodetectors
Elsayed Elgazzara. MehmetOzdemirb.HakanUstab.Ahmed A.Al-Ghamdic. A.Dered. FaridEl- Tantawya.F.Yakuphanogluc,d,e aDepartment of Physics, Faculty of Science, Suez Canal University, Ismailia, Egypt bDepartment of Materials Science and Nanotechnology Engineering, Abdullah Gül University, Kayseri, Turkey cPhysics Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia dDepartment of Physics, Faculty of Science, Fırat University, Elazig, Turkey eNanoscience and Nanotechnology Laboratory, Fırat University, Elazig, Turkey
Abstract
A novel photodetector of Al/GO:C8-BTBT/n-Si/Au with various graphene oxide (GO) contents has been investigated. The electrical properties of the diodes were characterized by current–voltage (I–V) and capacitance–voltage (C–V) measurements. The values of barrier height, ideality factor, and series resistance of the diodes were determined from I–V characteristic curves by using Norde‟s equations. The photocurrent properties of the diode were studied under various illumination intensities. The photoconducting mechanism of the diodes is controlled by the traps. The photoresponse properties of the diodes are increased with GO contents. The obtained results indicate that graphene oxide doped 2,7-dioctyl[1]benzothieno[3,2-b][1]benzothiophene/n-Si heterojunctions can be used as a photodetector for optoelectronic applications.
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Published Research Articles in International Journals 2014-2015
Journal of Geophysics and Engineering 2015 12:897–908
GPR background removal using a directional total variation minimisation approach Essam A Rashed
Image Science Lab., Department of Mathematics, Faculty of Science, Suez Canal University, Ismailia
Abstract
Ground penetrating radar (GPR) is a leading geophysical subsurface imaging tool for various purposes. This efficiency, however, is compromised by the interference of different types of noise. Background noise (clutter) is one of the nagging types of noise that undermines thehigh-resolution imaging capabilities of GPR. This study presents the experience of applyinga directional total variation minimisation (DTVM) filter to attenuate clutter in GPR data. The application of DTVM to both synthetic and field GPR data proves its great capability to attenuate clutter without affecting the features of interest of a GPR section. The results alsoshow that the proposed DTVM method affords superior image quality than both the mostcommonly used and the most recently published background removal techniques.
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Published Research Articles in International Journals 2014-2015
Russian Journal of General Chemistry 2014 84:1841–1846
Synthesis and Spectroscopic Characterization of Zn(II), Cd(II), and Hg(II) Ciprofloxacin Complexes
Mahmoud Salmana, Moamen S. Refatb,c, Sabry A. El-Korashyd, and Mostafa A. Hussienb
a Department of Chemistry, Faculty of Science, Al-Hussein Bin Talal University, P.O. Box 20 Ma‟an, Jordan b Chemistry Department, Faculty of Science, Port-Said University, Port-Said, Egypt e-mail: [email protected] c Department of Chemistry, Faculty of Science, Taif University, Al-Haweiah, P.O. Box 888, Zip Code 21974, Taif, Saudi Arabia dChemistry Department, Faculty of Sciences, Suez Canal University, Ismailia, Egypt
Abstract
Ciprofloxacin metal complexes with general formula [M(CPF)2]X2·nH2O [M = Zn(II), Cd(II), and Hg(II)] have been synthesised and characterized using elemental analysis (CHN), spectroscopic (UV-Vis, IR, MS, and 1H NMR) and thermogravimetric (TG and DTA) data. Using the Coats–Redfern and Horowitz–Metzeger methods, kinetic analysis of the thermogravimetric data had been performed.
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Published Research Articles in International Journals 2014-2015
Journal of Applied Geophysics 2014 106: 154–163
lavomer dnuorgkcab levon A :)SMB( noitcartbus xirtam dnuorgkcaB atad RPG rof mhtirogla.
Mohamed Rashed a,b,⁎, Hussein Harbi a a Department of Geophysics, King Abdulaziz University, Jeddah, Saudi Arabia b Geology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt
Abstract Background noise is a common type of coherent noise that severely compromises the integrity of the high- resolution images provided by ground penetrating radar survey. Several existing techniques employ different approaches to attenuate background noise. In this study, we present the background matrix subtraction (BMS) as an alternative technique to remove horizontal background noise and we compare its efficiency to that of the conventional background removal technique. Instead of calculating an average trace that is subtracted from the GPR data in the conventional background removal methods, the BMS technique is based on calculating a complete background matrix of the same size of the GPR section. The background matrix is created through a series of windowing, sample exclusion, weighting, and iteration. This series of processes guarantees that the background matrix is least affected by target response and is composed purely of horizontal background noise. The computed background matrix is then subtracted from the GPR data to remove horizontal events. Results of experiments conducted on both synthetic and real GPR data show that the BMS technique yields better results than the commonly used background removal technique.
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Published Research Articles in International Journals 2014-2015
Frontiers in Microbiology 2015
Trichoderma volatiles effectingArabidopsis: from inhibition toprotection against phytopathogenicfungi
Metwally Kottb 1 †, Tamara Gigolashvili 2, Dominik K. Großkinsky 3, 4 and Birgit Piechulla 1*
1 Institute for Biological Sciences, University of Rostock, Rostock, Germany, 2 Biocenter, Botanical Institute and Cluster ofExcellence on Plant Sciences, University of Cologne, Cologne, Germany, 3 Department of Plant and Environmental Sciences,Copenhagen Plant Science Centre, University of Copenhagen, Taastrup, Denmark, 4 Institute of Plant Sciences, University ofGraz, Graz, Austria
Abstract
Trichoderma species are present in many ecosystems and some strains have the abilityto reduce the severity of plant diseases by activating various defense pathways viaspecific biologically active signaling molecules. Hence we investigated the effects of lowmolecular weight volatile compounds of Trichoderma asperellum IsmT5 on Arabidopsisthaliana. During co-cultivation of T. asperellum IsmT5 without physical contact toA. thaliana we observed smaller but vital and robust plants. The exposed plants exhibitincreased trichome numbers, accumulation of defense-related compounds such asH2O2, anthocyanin, camalexin, and increased expression of defense-related genes. Weconclude that A. thaliana perceives the Trichoderma volatiles as stress compoundsand subsequently initiates multilayered adaptations including activation of signalingcascades to withstand this environmental influence. The prominent headspace volatileof T. asperellum IsmT5 was identified to be 6-pentyl-a-pyrone (6PP), which was solelyapplied to A. thaliana to verify the growth and defense reactions. Most noticeable is thatA. thaliana preexposed to 6PP showed significantly reduced symptoms when challengedwith Botrytis cinerea and Alternaria brassicicola, indicating that defense-activated plantssubsequently became more resistant to pathogen attack. Together, these results supportthat products that are based on Trichoderma volatiles have the potential being a usefulbiocontrol agent in agriculture.
Pag 86
Published Research Articles in International Journals 2014-2015
International Journal of Pest Management 2015 (61) 121-125
The acaricidal activity of some essential and fixed oils against the two- spotted spider mite in relation to different temperatures.
Manal S.M. Ismaila, Maha F.M. Solimanb*, Ahmed H. Abo-Ghaliab& Mona M.A. Ghallaba bDepartment of Zoology, Suez Canal University, Ismailia, Egypt
Abstract
The acaricidal activity of some essential and fixed oils against the two-spotted spider mite, Tetranychus urticae Koch were evaluated at 15 °C, 27 °C and 35 °C under laboratory conditions. The essential oils namely, rosemary (0.125%), garlic (0.5%), jojoba (0.25%) and the fixed vegetable oil (soyabean and sunflower oil (0.125%)) were tested in this study. The acaricidal activity was evaluated by studying the mortality, fecundity and fertility rates of the adult females at three different temperatures. Results showed that highest mortality rates among T. urticae were recorded for garlic oil followed by vegetable, jojoba and rosemary oils at 15 °C and for garlic, jojoba, rosemary and vegetable oils at 35 °C. Interestingly, no mortality rates were recorded for all the tested oils at 27 °C. At 15 °C, garlic oil caused a significant effect on both the fecundity and fertility rates as compared to the other oils. On the other hand, at 27 °C and 35 °C, most of the tested oils caused significant effects on the fecundity and fertility rates. In conclusion, the temperature is apparently a key factor regulating the acaricidal activity of the tested oils and attention must be given to this factor during field applications.
Pag 87
Published Research Articles in International Journals 2014-2015
Environ Earth Sci
2015
Optimization of municipal landfill siting in the Red Sea coastaldesert using geographic information system, remote sensingand an analytical hierarchy process.
Hesham Abd-El Monsef Suez Canal University, Ismailia, Egypt
Abstract
This paper describes an analytical approach tothe use of geographic information system (GIS), remotesensing, and an Analytical Hierarchy Process (AHP) toidentify and locate suitable locations for new landfill sitesin a rapidly growing tourist destination along coastal desertregions of the Red Sea in Egypt. The study took into accountfactors including the proximity of municipal landfillsites to transportation routes, hotels, tourist resorts, airports,the coast, groundwater, high flood risk zones, faultsand fractures, and residential communities. These factorsor criteria were categorized into three regularity groups.Experts from governmental agencies, non-governmentalorganizations, and academic institutions scored the criteriagrids and determined the relative importance ratio of eachpair of criteria inside regularity groups and the relativeimportance of each pair of the regularity groups. Then, anAHP ArcGIS extension was applied using pairwise comparisonmatrices to determine the weight of individualcriterion in each regularity group and the weight of theregularity groups. The weighted scored grid of municipallandfill siting criteria was used to locate regions that werehighly conformal with all the criteria. Three suitable municipalsites were delineated out of these regions by a fieldsurvey.
Pag 88
Published Research Articles in International Journals 2014-2015
BMC Complementary and
Alternative Medicine 2015 15:252
Hepatoprotective effect of mulberry (Morusnigra) leaves extract against methotrexateinduced hepatotoxicity in male albino rat.
Hend M. Tag Zoology Department, Faculty of Sciences, Suez Canal University, Ismaillia 41522, Egypt
Abstract Background: Drug-induced liver injury is a major health problem that challenges not only health care professionals but also the pharmaceutical industry and drug regulatory agencies. The possible hepatoprotective effect of the administration of mulberry ethanolic extract (MUL) leaves against hepatotoxic effect of the anti-rheumatic drug, methotrexate (MTX) was evaluated in this study both vivo (using animal models) and in vitro (human hepatoma HepG2 cells). Methods: In the in-vivo study, twenty male albino rats were equally assigned into four groups; control group received distilled water orally; MUL treated-group received 500 mg/kg/day of MUL extract; MTX treated- group was injected with a single dose of 20 mg/kg MTX intraperitoneally on the 4th day; MUL-MTX treated- group received the previously mentioned doses of MUL and MTX (both control and MUL treated groups were administered a single dose of a physiological saline i.p.). At the end of the experimental period (14 days) activities of alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) as well as total serum protein (TP) and albumin (ALB) levels were evaluated to assess liver function. Results: A marked reduction in the viability of HepG2 cells was observed after 48 h with IC50 equal to 14.5 μg/mL of MUL administration. Treating the animals with MUL in combination with MTX mitigated liver injury, causing a significant reduction in activities of AST, ALT, ALP and LDH as compared to the MTX-group. The liver architecture revealed more or less normal appearance with the combined treatment when compared with MTX treatment alone. Conclusions: This study recommends that the co-administration of MUL with MTX hat may have therapeutic benefits against MTX-hepato-cytotoxicity.
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Published Research Articles in International Journals 2014-2015
Letters in Drug Design and Discovery 2015 12, 211-218
Biological Effect of Glycosyl-Oxadiazolinethione and Glycosyl-sulfanyloxadiazole Derivatives through their in vitro Inhibition of Glycosidases from Bacteria and Normal or Diabetic Rats.
Ahmed T. Boraei Department og Chemistry, Faculty of Science, Suez Canal University. Ismailia, Egypt
Abstract
The inhibition of glycosidases from bacteria and the liver of normal and diabetic rats by 2- (tetra-O-acetyl-_-D-glucopyranosylsulfanyl)-5-(1-benzyl-1H-indol-2-yl)-1,3,4-oxadiazole nM- 3B;3-(2,3,4,6-tetra-O-acetyl-_-D-galacto-pyranosyl)-5-(1H-indol-2-yl)-1,3,4-oxadiazole- 2(3H)- thione MTB-4A; 3-(2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-_-Dglucopyranosyl)-5-(1-benzyl- 1H-indol-2-yl)-1,3,4-oxadiazole-2(3H)-thioneBnN-5A has been investigated. In vitro treatmentofhepatic-amylaseand-glucuronidasefromcontrolandstreptozotocin- induceddiabeticratsby S-andN- glycosylanaloguesfromoxadiazolinethionederivativesexhibitedasignificantdose- dependentdecreaseon thespecificactivityofboth -amylase and -glucuronidase.Moreover, these compounds also exhibited a significant decrease on the specific activity of_- amylase and_-glucosidaseproducedby BacillussubtilisAH.
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Published Research Articles in International Journals 2014-2015
Journal of Petroleum Geology 2014 (2), 183-195
PETROPHYSICAL CHARACTERISTICS OF THE MESSINIAN ABU MADI FORMATION IN BALTIM EAST AND NORTH FIELDS, OFFSHORE NILE DELTA, EGYPT
M. I. Abdel-Fattah*
* Geology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt.
Abstract Baltim East and North fields in the offshore Nile Delta produce from gas-condensate accumulations located in the northern portion of the Abu Madi palaeovalley. Gas accumulations in Abu Madi Formation occur in sandstone reservoir units referred to as the Level III Main and Level III Lower. The petrophysical characteristics of these reservoir units in the Baltim area are described using data from wireline logs (gamma-ray, density, neutron, sonic and resistivity) from fourteen wells and core data from one well.
Results of wireline log and core analyses indicate that the Level III Main can subdivided into two sandstone-dominated intervals (both interpreted as sandbar deposits) separated by a shale-rich interval which is a partial barrier to fluid flow. Effective porosity is 9-18.5% and permeability 40-100 mD. Sandstones in the Level III Lower are interpreted as braided channel facies and have effective porosity of 12.5-22% and permeability of 100 - 500 mD.
Iso-petrophysical maps for the Abu Madi Formation sandstone reservoirs based on log and core interpretations show the impact of depositional facies on petrophysical characteristics to assess thepossible future exploration and development targets.
Pag 91
Published Research Articles in International Journals 2014-2015
Environmental Science and Polluttion Research 2015 22:3082–3097
Assessment of natural and artificial radioactivity levels and radiation hazards and their relation to heavy metals in the industrial area of Port Said city, Egypt
T. E. Attia &E. H. Shendi& M. A. Shehata Geology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt
Abstract A detailed gamma ray spectrometry survey was carriedout to make an action in environmental impact assessmentof urbanization and industrialization on Port Said city, Egypt.The concentrations of the measured radioelements U-238, Th-232 in ppm, and K-40 %, in addition to the total counts of threeselected randomly dumping sites (A, B, and C) were mapped.The concentration maps represent a base line for the radioactivityin the study area in order to detect any future radioactivecontamination. These concentrations are ranging between 0.2and 21 ppm for U-238 and 0.01 to 13.4 ppm for Th-232 as wellas 0.15 to 3.8 % for K-40, whereas the total count values rangefrom 8.7 to 123.6 uR. Moreover, the dose rate was mappedusing the same spectrometer and survey parameters in order toassess the radiological effect of these radioelements. The doserate values range from 0.12 to 1.61 mSv/year. Eighteen soilsamples were collected from the sites with high radioelementconcentrations and dose rates to determine the activity concentrations of Ra-226, Th-232, and K-40 using HPGe spectrometer.The activity concentrations of Ra- 226, Th-232, and K-40 in themeasured samples range from 18.03 to 398.66 Bq kg−1, 5.28 to 75.7 Bq kg−1, and 3,237.88 to 583.12 Bq kg−1, respectively. Inaddition to analyze heavy metal for two high reading samples(a1 and a10) which give concentrations of Cd and Zn elements(a1 40 ppm and a10 42 ppm) and (a1 0.90 ppm and a100.97 ppm), respectively, that are in the range of phosphatefertilizer products that suggested a dumped man-made wastein site A. All indicate that the measured values for the soilsamples in the two sites of three falls within the world rangesof soil in areas with normal levels of radioactivity, while site Ashows a potential radiological risk for human beings, and it isimportant to carry out dose assessment program with a specificallydetailed monitoring program periodically.
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Published Research Articles in International Journals 2014-2015
Journal of Molecular Liquids 2015 211:126–134
Synthesis, surface properties and inhibition behavior of novel cationic gemini surfactant for corrosion of carbon steel tubes in acidic solution.
M.A. Hegazya,*, S. Rashwan, H. b, M. Kamelb, M.S. – El Kotbb aEgyptian Petroleum Research Institute (EPRI), Nasr City, Cairo, Egypt bChemistry Dept., Faculty of Science, Suez canal University, Ismailia, Egypt
Abstract
In the present investigation nnovel gemini surfactantnamely: diethyl oxalatebis (N,N- dimethyldodecyl bromide), was synthesized, characterized, and tested as a corrosion inhibitor for carbon steel in 1 M HCl solution. The corrosion inhibition efficiency was determined by using electrochemical impedance spectroscopy (EIS), potentiodynamic polarization, and weight loss methods. From the results,it was clear that the synthesized inhibitor is an excellent inhibitor for carbon steel in 1 M HCl solution. The inhibition efficiency increases by increasing the concentration of the inhibitor. Thermodynamic and activation parameters were discussed. The synthesized inhibitor‟s adsorption was found to follow the Langmuir‟s adsorption isotherm.
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Published Research Articles in International Journals 2014-2015
Experimental and Toxicologic Pathology 2014 67: 65–76
Effect of vitamin E and selenium separately and in combination on biochemical, immunological and histological changes induced by sodium azide in male mice.
Nahla S. El-Shenawya,∗, Mohammad S. AL-Harbib, Reham Z. Hamzac
Zoology Department, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt, bBiology Department, Faculty of Science, Taif University, Taif 888, Saudi Arabia, cZoology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt .
Abstract Sodium azide (SA) is used as an active ingredient to control a broad spectrum of soil borne pathogens including insects, weeds, nematodes, fungi, and bacteria. The purpose of this study was to evaluate the ameliorator property of vitamin E (Vit E) or/and selenium (Se) against SA-induced injury in male mice at the biochemical, immunological and histological levels. The mice were divided into nine groups (10/group). The first three groups were served as control, Vit E and Se while, the second three groups were treated with three different doses of SA. The last three groups were treated with high dose of SA with Vit E or Se or Vit E and Se and all animals were treated for a period of 30 days. Exposure to SA at the three doses to mice led to an alternation of liver and kidney functions, decrease the testosterone concentration, decreased IgG and IgM levels as well as the increasing the TNF- α. The effects of SA on thebiochemical parameters of mice were dose-dependent. Administration of Se or/and Vit E to SA-treated mice attenuates the toxicity of this compound, objectified by biochemical and histological improvement of liver, kidney and testis. But, the alleviation is more pronounced with the both antioxidants. Thus, the synergistic effect of Se and Vit E is most powerful in reducing the toxicity induced by SA and improving the humoral immune response of mice.
Pag 94
Published Research Articles in International Journals 2014-2015
Journal of Arid Environments 2015 112: 170-176
Snapshots of scorpion venomics.
Mohamed A. Abdel-Rahman*a, Patrick L. Harrison b, Peter N. Strong b aZoology Department, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt bBiomedical Research Centre, Biosciences Division, Sheffield Hallam University, Sheffield S1 1WB, UK
Abstract Scorpions are particularly well adapted to survival in extreme habitats (especially arid and semi-arid environments) and their ability to produce and deliver venoms is an important factor in this success. Scorpion venoms are very complex mixtures of different proteins and peptides. Previous venomics studies revealed that each one of scorpion species may contain more than 100 different peptides. Scorpion venom peptides can be classified into two main types: disulfide-bridged peptides (DBPs) and non-disulfide- bridged peptides (NDBPs). The vast majority of DBPs are neurotoxic peptides that specifically interact with various types of ion-channels. The NDBPs have been shown to variously possess bradykinin-potentiating, antimicrobial, hemolytic, cellular signaling and immune-modulating activities. Recently, venom proteomics have been extensively applied in assessing the diversity of scorpion venom from various species. More insights about scorpion venom compositions were also gained through transcriptomic approach. It has provided an opportunity to obtain an overview of the content of scorpion venoms and to compare the relative abundance of toxin transcripts. More importantly, transcriptomics can reflect the biological processes occurring in venom gland cells. This review will highlight recent proteomic and transcriptomic studies to explore the venome of scorpions from different habitats, focusing on desert scorpions from North Africa.
Pag 95
Published Research Articles in International Journals 2014-2015
Gondwana Research
2015
Time constraints on deformation of the Ajjaj branch of one of the largest Proterozoic shear zones on Earth: The Najd Fault System.
Mahmoud HASSAN Faculty of Science, Suez Canal University, Ismailia, EGYPT
Abstract
In active tectonic regions, shear zones play an important role in re-configuring the structure of the lithosphere. One of the largest shear zones on Earth is the Najd Fault System of the Arabian-Nubian Shield. The main active phase of this shear zone was during the last stages of the Pan-African Orogeny (ca. 630 - 540 Ma). Six samples of intrusive rocks that were emplaced into the shear zone at different stages during its active phase are used to illustrate the progressive evolution of the Ajjaj shear zone. A sample of coarse- grained diorite, with an intercept U-Pb zircon age of 696 ± 6 Ma, shows very weak deformation. Two samples from deformed granodiorite-tonalite intrusions at the border of the Ajjaj shear zone show conspicuous degrees of deformation, and define two U-Pb clusters of concordia ages at 747 ± 12 Ma - 668 ± 8 Ma and 742 ± 5 Ma - 702 ± 12 Ma. Two samples are granites show mylonitic foliation with flattened quartz and biotite parallel to the trend of the shear zone. These samples yield U-Pb ages of 601± 3 Ma - 584 ± 3 Ma. Another granite sample is undeformed and shows cross-cutting relations with the shear foliation of the Ajjaj shear zone. It yields a concordia age of 581 ± 4 Ma. The metamorphic rocks of the Hamadat complex host the Ajjaj shear zone, and have been useful in determining the metamorphic PT conditions attending the activity of the shear zone. The peak metamorphism of the Hamdat Complex is 505 - 700 ºC at two ranges of pressure 8 - 11 and 14.5 ± 2 kbar. New data confine the activation of the Ajjaj shear zone in a limited period of time between 604 Ma and 581 Ma and the operation at different crustal levels with a maximum depth of 58 km.
Pag 96
Published Research Articles in International Journals 2014-2015
Botanical Studies 2014 55:53
Differentiation between two strains of microalga Parachlorella kessleri using modern spectroscopic method
Marwa M Saleh13*, Dmitry N Matorin2, Bolatkhan K Zayadan1, Daria A Todorenko2, Evgenii P Lukashov2 and Mona M Gaballah3
1Department of Biotechnology, Al-Farabi Kazakh National University, 71,Al-Farabi, ave, Almaty 050040, Kazakhstan, 2 Faculty of Biology, Moscow StateUniversity, Vorobyevi Gory 119992, Moscow, Russia,3 Botany Department, Faculty of Science, Suez Canal University, Ismaillia41522, Egypt.
Abstract
Background: The differentiation between wild type of Parachlorella kessleri and its mutant strains PCMut2, PCMut4 by using the Multi-functional Plant Efficiency Analyzer (М-РЕА-2) was studied. Mutant algal cells of P. kessleri have been obtained by UV-C during 3 and 10 min respectively. Results: Light-induced kinetics of prompt fluorescence (OJIP transients), delayed fluorescence and modulated reflection at 820 nm (redox transitions of P700 in PSI) showed disturbance of electron transport flow in photosystem II (PSII) and an increase fraction of non-reducing centers of secondary quinone acceptors of electron (QB). In addition, the amplitudes of the fast and slow peak in the kinetics of the delayed light emission and non- photochemical fluorescence quenching (NPQ) were significantly reduced in mutant cells, indicating low level of the membrane energization of photosynthetic membranes. Changes of photosynthetic reactions of mutants may lead to an increase of the carotenoids content, which protect cells against the light stress. Conclusion: It is suggested to use parameters of induction curves of prompt and delayed fluorescence to characterize mutant algal cells in biotechnological studies.
Pag 97
Published Research Articles in International Journals 2014-2015
Journal of Environmental Management 2015 (169)202-209
Extensive management of field margins enhances their potential for off-site soil erosion mitigation
Hamada E. Ali a, b, *, Bj€orn Reineking a, c, d a Biogeographical Modelling, Bayreuth Center of Ecology and Environmental Research BayCEER, University of Bayreuth, Universit€atsstraße 30, D-95440 Bayreuth, Germany b Botany Department, Faculty of Science, Suez Canal University, 41522 Ismailia, Egypt c Irstea, UR EMGR, 2 rue de la Papeterie-BP 76, F-38402 St-Martin-d'H_eres, France d Univ. Grenoble Alpes, F-38402 Grenoble, France
Abstract Soil erosion is a widespread problem in agricultural landscapes, particularly in regions with strong rainfall events. Vegetated field margins can mitigate negative impacts of soil erosion off-site by trapping eroded material. Here we analyse how local management affects the trapping capacity of field margins in a monsoon region of South Korea, contrasting intensively and extensively managed field margins on both steep and shallow slopes. Prior to the beginning of monsoon season, we equipped a total of 12 sites representing three replicates for each of four different types of field margins (“intensive managed flat”, “intensive managed steep”, “extensive managed flat” and “extensive managed steep”) with Astroturf mats. The mats (n = 15 / site) were placed before, within and after the field margin. Sediment was collected after each rain event until the end of the monsoon season. The effect of management and slope on sediment trapping was analysed using linear mixed effects models, using as response variable either the sediment collected within the field margin or the difference in sediment collected after and before the field margin. There was no difference in the amount of sediment reaching the different field margin types. In contrast, extensively managed field margins showed a large reduction in collected sediment before and after the field margins. This effect was pronounced in steep field margins, and increased with the size of rainfall events. We conclude that a field margin management promoting a dense vegetation cover is a key to mitigating negative off-site effects of soil erosion in monsoon regions, particularly in field margins with steep slopes. Pag 98
Published Research Articles in International Journals 2014-2015
Chemical and Pharmaceutical bulletin of Japan 2014 (2)675-694
Molecular modeling studies and synthesis of novel methyl 2-(2-(4-oxo-3- aryl-3,4-dihydroquinazolin-2-ylthio)acetamido) alkanoates with potential anti-cancer activity as inhibitors for methionine synthase
Ismail Mahmoud Elfekki1), Walid Fathalla Mohamed Hassan2), Hosam Eldin Abd Elhamed Elshihawy3), Ibrahim Ahmed Ibrahim Ali1),Elsayed Hussein Mostafa Eltamany1)
1) Department of Chemistry, Faculty of Science, Suez Canal University 2) Department of Physics and Math. Engineering, Faculty of Engineering, Port-Said University 3) Department of Organic Chemistry, Faculty of Pharmacy, Suez Canal University
Abstract
Cobalamin-dependant cytosolic enzyme methionine synthase (MetS) catalyses the transfer of a methyl group from the methyltetrahydrofolate (MTHF) to homocysteine (Hcy) to produce methionine and tetrahydrofolate (THF). MetS is over-expressed in the cytosol of certain breast and prostate tumour cells. Methionine used as a source of one carbon atom for the building of the DNA of the tumour cells, structural protein and enzymes. In this study, we designed, synthesized and evaluated the cytotoxic activity of a series of substituted methyl 2-(2-(4-oxo-3-aryl-3,4-dihydroquinazolin-2-ylthio)acetamido)acetate and dipeptide that mimic the substructure of MTHF. These inhibitors w ere docked in to the MTHF binding domain in such the same way as MTHF in its binding domain. The free energies of the binding were calculated and compared to the
IC50 values. This series has been developed by dicyclohexylcarbodiimide (DCC) and azide coupling methods of amino acid esters with carboxylic acid derivatives, respectively. Compound methyl 3-hydroxy-2-(2-(3-(4-methoxyphenyl)-4-oxo-3,4-dihydroquinazolin-2- ylthio) acetamido) propanoate exhibited the highest IC50 value 20 µg/mL against PC-3 cell line and scored the lowest free energy of the binding (−207.19 kJ/mol)
Pag 99
Published Research Articles in International Journals 2014-2015
Pakistan Journal pf Botany 2015 (3): 995-1006
GENOTOXIC EFFECT OF PEGANUM HARMALA EXTRACTS ON THE GROWTH OF VICIA FABA L. AND DNA USING NUCLEAR MICROSATELLITES
LAILA MEKKI*1,MOHAMMED A. HUSSEIN2 AND HASSAN MANSOUR1
1Department of Botany, Faculty of Science, Suez Canal University, Ismailia Egypt 2Department of Botany, Faculty of Agriculture, Suez Canal University Ismailia, Egypt
Abstract Assessment of genotoxins-induced DNA damage and mutations at molecular level is important in ecogenotoxicology. Treatment with Peganum harmala crude extract can result in cytotoxic, carcinogenic and mutagenic effects. Mutagenesis is indicative of genetic instability and can be assayed using microsatellites or simple sequence repeats (SSRs). The application of SSR marker analysis using capillary electrophoresis (CE) apparatus showed differences in SSR profiles within the extract as well as between the two extracts. All the 12 SSR primer pairs used produced amplification products which showed 100% polymorphism. The total number of scoreable loci obtained with the 12 used primers were 112 alleles. Of the 112 alleles detected 12 alleles (10.7 %) were considered as zero alleles. Three alleles produced by various primers were detected at locus VfG_22 to 15 at locus VfG_693 and VfG_873, with an average of 9.3 bands/SSR. The discrimination power of each SSR locus was estimated by the PIC, which ranged from 0.47 for locus VfG_22 to 0.88 for locus VfG_693 with a mean of 0.70. About 83.3% of the 18 alleles observed in untreated samples using the 12 primers disappeared from almost all the treatments with water and ethanol extracts. Statistical analysis of morphometric traits showed significant differences between the two extracts in all studied traits except the percentage of germination and root dry weight which were non significant. However, on the other hand significant differences were observed between treatments and the control for all the traits studied.
Pag100
Published Research Articles in International Journals 2014-2015
PLOS ONE 2015
DJ-1 Protects Pancreatic Beta Cells from Cytokine- and Streptozotocin- Mediated Cell Death
Amir E. Mehana4,5 4 Division of Endocrinology and Diabetology, Department of Internal Medicine II, University Hospital of Freiburg, Freiburg, Germany, 5 Department of Zoology, Faculty of Science, Suez Canal University, Ismailia, Egypt,
Abstract A hallmark feature of type 1 and type 2 diabetes mellitus is the progressive dysfunction and loss of insulin-producing pancreatic beta cells, and inflammatory cytokines are known to trigger beta cell death. Here we asked whether the anti-oxidant protein DJ-1 encoded by the Parkinson‟s disease gene PARK7 protects islet cells from cytokine- and streptozotocin mediated cell death. Wild type and DJ-1 knockout mice (KO) were treated with multiple low doses of streptozotocin (MLDS) to induce inflammatory beta cell stress and cell death. Subsequently, glucose tolerance tests were performed, and plasma insulin as well as fasting and random blood glucose concentrations were monitored. Mitochondrial morphology and number of insulin granules were quantified in beta cells. Moreover, islet cell damage was determined in vitro after streptozotocin and cytokine treatment of isolated wild type and DJ-1 KO islets using calcein AM/ethidium homodimer-1 staining and TUNEL staining. Compared to wild type mice, DJ-1 KO mice became diabetic following MLDS treatment. Insulin concentrations were substantially reduced, and fasting blood glucose concentrations were significantly higher in MLDS-treated DJ-1 KO mice compared to equally treated wild type mice. Rates of beta cell apoptosis upon MLDS treatment were twofold higher in DJ-1 KO mice compared to wild type mice, and in vitro inflammatory cytokines led to twice as much beta cell death in pancreatic islets from DJ-1 KO mice versus those of wild type mice. In conclusion, this study identified the anti-oxidant protein DJ-1 as being capable of protecting pancreatic islet cells from cell death induced by an inflammatory and cytotoxic setting.
Pag101
Published Research Articles in International Journals 2014-2015
Earth-Science Reviews 2014 (136)289–300
The Use of GRACE Data to Monitor Natural and Anthropogenic Induced Variations in Water Availability Across Africa
Mohamed Ahmed a,b, Mohamed Sultan a,⁎, John Wahr c, Eugene Yand a Department of Geosciences, Western Michigan University, 1903 West Michigan Avenue, Kalamazoo, MI 49008, USA b Department of Geology, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt c Department of Physics, University of Colorado at Boulder, 2000 Colorado Avenue, Boulder, CO 80309, USA d Environmental Science Division, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439, USA
Abstract Inter-annual trends in terrestrialwater storage (TWS)were extracted frommonthly (01/2003– 09/2012) GravityRecovery and Climate Experiment (GRACE) data acquired over Africa and correlated (in a geographic informationsystem [GIS] environment) with relevant temporal remote sensing, geologic, and hydrologic datasets. Findingsinclude the following: (1) large sectors of Africa are undergoing statistically significant TWS variations(+44 mm/yr to −15 mm/yr) due to natural and anthropogenic causes; (2) warming of the tropical AtlanticOcean apparently intensified Atlantic monsoons and increased precipitation and TWS over western and centralAfrica; (3) warming in the central Indian Ocean decreased precipitation and TWS over eastern Africa; (4) thehigh frequency of flooding events increased TWS over the Zambezi and Okavango basins; (5) extraction of fossilgroundwater decreased TWS over the Saharan aquifers; (6) deforestation decreased TWS in three subbasins(Ubangi, Congo, and Sangha) of the Congo River Basin; and (7) the construction of dams increased TWS inthe Blue Nile and Atbara subbasins. Given the 10 years of monthly GRACE data acquired on the subbasinscale across the globe, as well as the plans underway for deployment of a GRACE-FO and GRACE-II, usingGRACE-derived TWS data should be considered an alternative, viable index for measuring temporal and spatialvariations in water availability.
Pag102
Published Research Articles in International Journals 2014-2015
BMC Complementary and Alternative Medicine 2015 15:301
Anticancer and apoptotic effects on cellproliferation of diosgenin isolated from Costus speciosus (Koen.) Sm
Samy Selim1,3* and Soad Al Jaouni2 1Department of Clinical Laboratory Sciences, College of Applied MedicalSciences, Aljouf University, Sakaka P.O. 2014, Saudi Arabia 3Microbiology and Botany Department, Faculty of Science, Suez CanalUniversity, Ismailia P.O. 41522, Egypt
Abstract Background: Diosgenin, a naturally occurring steroid saponin found abundantly in C. speciosus, is a well-known precursor of various synthetic steroidal drugs that are extensively used in the pharmaceutical industry. Methods: The present study was conducted to evaluate the in vitro anticancer and apoptotic effects on cell proliferation of diosgenin isolated from C. speciosus (Koen.) Sm. Results: The results indicated that the treatment of HepG2 cells with the sample resulted in a cytotoxic effect as concluded from the IC50 value 32.62 μg/ml, while the treatment of
HepG2 cells with paclitaxel, a known anti-cancer drug, resulted in an IC50 value of 0.48 μg/ml. The treatment of MCF-7 cells with the tested sample resulted in high inhibition in the cell viability, and resulted in an IC50 value of 11.03 μg/ml, while the treatment of MCF-
7 cells with paclitaxel resulted in an IC50 value of 0.61 μg/ml. The levels of DR4 and caspase-3 were significantly increased (P < 0.01) in MCF-7 cells treated with the tested sample compared to untreated cells and possessed a similar activity of paclitaxel in DR4 induction but lower induction in caspase-3. On the other hand the treatment of macrophages or lymphocytes with diosgenin (250 μg/ml) resulted in an induction in the cell proliferation up to 3.2-fold and 2.1-fold of control, respectively. Conclusions: The results presented here may suggest that diosgenin isolated from C. speciosus possess anticancer and apoptotic effects on cell proliferation, and therefore, can be used as pharmaceuticals drugs.
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Published Research Articles in International Journals 2014-2015
Molecules 2015 20, 13620-13641
Metabolic Analysis of Various Date Palm Fruit (Phoenix dactylifera L.) Cultivars from Saudi Arabia to Assess Their Nutritional Quality
Ismail Hamad 1,6,†, Hamada AbdElgawad 2,3,†, Soad Al Jaouni 4, Gaurav Zinta 2, Han Asard 2, Sherif Hassan 1,3, Momtaz Hegab 3, Nashwa Hagagy 5 and Samy Selim 1,5,*
1 Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Aljouf University, Sakaka 2014, Saudi Arabia; E-Mails: [email protected] (I.H.); [email protected] (S.H.)
5 Microbiology and Botany Department, Faculty of Science, Suez Canal University, Ismailia 41522,Egypt; E-Mail: [email protected]
Abstract Date palm is an important crop, especially in the hot-arid regions of the world. Date palm fruits have high nutritional and therapeutic value and possess significant antibacterial and antifungal properties. In this study, we performed bioactivity analyses and metabolic profiling of date fruits of 12 cultivars from Saudi Arabia to assess their nutritional value. Our results showed that the date extracts from different cultivars have different free radical scavenging and anti-lipid peroxidation activities. Moreover, the cultivars showed significant differences in their chemical composition, e.g., the phenolic content (10.4–22.1 mg/100 g DW), amino acids (37–108 μmol·g−1 FW) and minerals (237–969 mg/100 g DW). Principal component analysis (PCA) showed a clear separation of the cultivars into four different groups. The first group consisted of the Sokary, Nabtit Ali cultivars, the second group of Khlas Al Kharj, Khla Al Qassim, Mabroom, Khlas Al Ahsa, the third group of Khals Elshiokh, Nabot Saif, Khodry, and the fourth group consisted of Ajwa Al Madinah, Saffawy, Rashodia, cultivars. Hierarchical cluster analysis (HCA) revealed clustering of date cultivars into two groups. The first cluster consisted of the Sokary, Rashodia and Nabtit Ali cultivars, and the second cluster contained all the other tested cultivars. These results indicate that date fruits have high nutritive value, and different cultivars have different chemical composition.
Pag104
Published Research Articles in International Journals 2014-2015
Biotechnology & Biotechnological Equipment 2015 1, 152-156
Rapid identification of Pseudomonas aeruginosa by pulsed-field gel electrophoresis
Samy Selima,b*, Iman El Kholyc, Nashwa Hagagyb, Sahar El Alfayb and Mohamed Abdel Azizb aDepartment of Clinical Laboratory Sciences, College of Applied Medical Science, Aljouf University, Sakaka, Saudi Arabia; bDepartment of Botany, Faculty of Science, Suez Canal University, Ismailia, Egypt; cClinical Pathology Department, Ain ShamsUniversity Specialized Hospital, Ain Shams University, Cairo, Egypt
Abstract Twenty clinical Pseudomonas aeruginosa isolates recovered from patients admitted to The General Hospital in Ismailia Governorate (Egypt) were examined in this study. We analysed P. aeruginosa ATCC 9027 (as a control strain) and 19 of the isolates after digestion with SpeI restriction endonuclease. After this we conducted a pulsed-field gel electrophoresis (PFGE) and typed the obtained 10 unique patterns, designated as A, A1, B, B1, C, C1, D, D1, E and F. We evaluated the genetic relatedness between all strains, based on _87% band identity. As a result, the isolates were grouped in the 10 clusters as follows: patterns A, A1, B, B1, C contained two strains each and patterns C1, D, D1, E contained a single strain each; the five remaining strains were closely related (genomic pattern F). One isolate belonged to antibiotype „b‟. The genotype patterns of the P. aeruginosa ATCC 9027 control strain and isolate no. 11 were closely related and had two different antibiotypes „d‟ and „c‟, respectively.
Pag105
Published Research Articles in International Journals 2014-2015
Future Medicinal Chemistry 2015
(15)1971-1980
A series of ceramide analogs modified at the 1-position with potent activity against the intracellular growth of Chlamydia trachomatis
Essa M Saied1,2, Sebastian Banhart3, Sophie E Bürkle3, Dagmar Heuer3 & Christoph Arenz*,1
1Institute of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2,12489 Berlin 2Chemistry Department, Faculty ofScience, Suez Canal University, Ismailia,Egypt 3Junior Research Group „SexuallyTransmitted Bacterial Pathogens‟ (NG 5),Robert Koch Institute, Berlin, Germany
Abstract
Background: Chlamydia trachomatis is an intracellular pathogen that requires different nutrients, including sphingolipids, for survival. Components for the transport and biosynthesis of sphingolipids thus may have a potential as antichlamydial targets. Results: In this study, we synthesized a collection of 24 ceramide derivatives. Three derivatives show pronounced activity with submicromolar IC50. The potency of these compounds was one order of magnitude higher than that of the antibiotic chloramphenicol. We show a detailed structure–activity relationship study for this novel compound class exhibiting a significant effect on the growth of C. trachomatis L2 without penetrating the bacteria itself. Conclusion: The structure–activity relationship presented here defines an important step toward the molecular target of this compound class, which is still elusive.
Pag106
Published Research Articles in International Journals 2014-2015
Plos one 2014
9(10): e108778
High Mortality of Red Sea Zooplankton under Ambient Solar Radiation
Ali M. Al-Aidaroos1*, Mohsen M. O. El-Sherbiny1,2, Sathianeson Satheesh1, Gopikrishna Mantha1, Susana Agustı¯3,4, Beatriz Carreja4, Carlos M. Duarte1,3,4
1 Department of Marine Biology, Faculty of Marine Sciences, King Abdulaziz University, Jeddah, Saudi Arabia, 2 Marine Science Department, Faculty of Science, Suez Canal University, Ismailia, Egypt, 3 The UWA Oceans Institute and School of Plant Biology, University of Western Australia, Crawley, WA, Australia, 4 Department of Global Change Research and LINC Global, IMEDEA (CSIC- UIB) Instituto Mediterra´neo de Estudios Avanzados, Miquel Marque´ s, Esporles, Spain
Abstract High solar radiation along with extreme transparency leads to high penetration of solar radiation in the Red Sea, potentially harmful to biota inhabiting the upper water column, including zooplankton. Here we show, based on experimental assessments of solar radiation dose-mortality curves on eight common taxa, the mortality of zooplankton in the oligotrophic waters of the Red Sea to increase steeply with ambient levels of solar radiation in the Red Sea. Responses curves linking solar radiation doses with zooplankton mortality were evaluated by exposing organisms, enclosed in quartz bottles, allowing all the wavelengths of solar radiation to penetrate, to five different levels of ambient solar radiation (100%, 21.6%, 7.2%, 3.2% and 0% of solar radiation). The maximum mortality rates under ambient solar radiation levels averaged (6standard error of the mean, SEM) 18.465.8% h21, five-fold greater than the average mortality in the dark for the eight taxa tested. The UV-B radiation required for mortality rates to reach Kof maximum values averaged (6SEM) 1265.6 h21% of incident UVB radiation, equivalent to the UV-B dose at 19.262.7 m depth in open coastal Red Sea waters. These results confirm that Red Sea zooplankton are highly vulnerable to ambient solar radiation, as a consequence of the combination of high incident radiation and high water transparency allowing deep penetration of damaging UV-B radiation. These results provide evidence of the significance of ambient solar radiation levels as a stressor of marine zooplankton communities in tropical, oligotrophic waters. Because the oligotrophic ocean extends across 70% of the ocean surface, solar radiation can be a globally-significant stressor for the ocean ecosystem, by constraining zooplankton use of the upper levels of the water column and, therefore, the efficiency of food transfer up the food web in the oligotrophic ocean.
Pag107
Published Research Articles in International Journals 2014-2015
IEEE SYSTEMS JOURNAL 2015 1-11
Integrating Nonfunctional Requirements Into Axiomatic Design Methodology
Mohamed A. Mabrok, Mahmoud Efatmaneshnik, and Michael J. Ryan Department of Mathematics, Faculty of Science, Suez Canal University, 41522 Ismailia, Egypt
Abstract Axiomatic design (AD) theory is a general framework for system design process based on mapping between several domains. In the AD theory, only the functional requirements (system functions) are considered in the design process, since the mapping between requirements and design parameters in AD does not include nonfunctional requirements (system attributes). This paper introduces an extended view of the system design process based on AD theory framework to incorporate nonfunctional requirements into the mapping between the problem domain and the solution domain. A systematic way of including nonfunctional requirements in the design process is presented, and an extended design matrix is introduced, which includes the mapping between a complete set of requirements (functional and nonfunctional) and corresponding design parameters (physical domain). We also present an approach to decide between solutions that satisfy nonfunctional requirements to a varying degree through the employment of the analytical hierarchy process.
Pag108
Published Research Articles in International Journals 2014-2015
Journal of Scheduling 2015 (6)645--653
Approximation algorithms for inventory constrained scheduling on a single machine. Ehab Morsy1,2 · Erwin Pesch3 1 Department of Management Information Science, SiegenUniversity, Holderlinstr. 3, 57068 Siegen, Germany 2 Department of Mathematics, Suez Canal University,Ismailia 22541, Egypt 3 Department of Management Information Science, SiegenUniversity, Kohlbettstr. 15, 57068 Siegen, Germany
Abstract
We consider the problem of scheduling a set of jobs on a single machine subject to inventory constraints, i.e., conditions that jobs add or remove items to or from a centralized inventory, respectively. Jobs that remove items cannot be processed if the required number of items is not available. We focus on scheduling problems on a single machine where the objective is to minimize the total weighted completion time. In this paper, we design 2-approximation algorithms for special cases of the problem that run in polynomial time.
Pag109
Published Research Articles in International Journals 2014-2015
Fractional calculus and Applied nalysis 2015 1252–1276
IMPLICIT DIFFERENCE SCHEME OF THE SPACE-TIME FRACTIONAL ADVECTION DIFFUSION EQUATION
Entsar A. A. Abdel-Rehim Department of Mathematics and Computer Science Faculty of Science, Suez Canal University Ismailia, EGYPT
Abstract
The space-time fractional advection diffusion equations are linear partialpseudo- differential equation with spatial fractional derivatives in timeand in space and are used to model transport at the earth surface. We usethe implicit difference scheme, the theta- method, to find the approximationsolution of these equations in the long run. The proofs of stability of thedifference scheme of each models are given. We compare the numerical resultsof these models for different values of the space and the time fractionalorders and for different values of theta.
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Published Research Articles in International Journals 2014-2015
Pag111
Published Research Articles in International Journals 2014-2015
Environ Monit Assess 2015 187: 583
Assessment of elemental and NROM/TENORM hazard potential from non- nuclear industries in North Sinai, Egypt.
A. F. El-Mekawy & H. M. Badran &M. K. Seddeek&T. Sharshar & T. Elnimr a Physics Department, Faculty of Science, Tanta University, Tanta, Egypt; b Physics Department, Faculty of Science, Tanta University, Tanta, Egypt; c Physics Department, Faculty of Education, Suez Canal University, Al-Arish, Egypt; ;Physics Department, Oklat Al-Skoor College of Science & Arts، Qassim University, Saudi Arabia d Physics Department, Faculty of Science, Kafrelsheikh University, Kafr El-Sheikh, Egypt; ;e Physics Department, Faculty of Science, Taif University, Al-Hawiya،Taif, Saudi Arabia f Physics Department, Faculty of Science, Tanta University, Tanta, Egypt;
Abstract Non-nuclear industries use raw materials containing significant levels of naturally occurring radioactive material (NORM). The processing of these materials may expose workers engaged in or even people living near such sites to technologically enhanced naturally occurring radioactive material (TENORM) above the natural background. Inductively coupled plasma and gamma ray spectrometry have been used to determine major and trace elements and radionuclide concentrations in various samples, respectively, in order to investigate the environmental impact of coal mining and cement plant in North Sinai, Egypt. Generally, very little attention was directed to the large volumes of waste generated by either type of industrial activities. Different samples were analyzed including various raw materials, coal, charcoal, Portland and white cement, sludge, and wastes. Coal mine and cement plant workers dealing with waste and kaolin, respectively, are subjected to a relatively high annual effective dose. One of the important finding is the enhancement of all measured elements and radionuclides in the sludge found in coal mine. It may pose an environmental threat because of its large volume and its use as combustion material. The mine environment may have constituted Al, Fe, Cr, and V pollution source for the local area. Higher concentration of Al, Fe, Mn, B, Co, Cr, Mn, Ni, Sr, V, and TENORM were found in Portland cement and Zn in white cement. Coal has higher concentrations of Al, Fe, B, Co, Cr, and Vaswell as 226Ra and 232Th. The compiled results from the present study and different worldwide investigations demonstrate the obvious unrealistic ranges normally used for 226Ra and 232Th activity concentrations in coal and provided ranges for coal, Portland and white cement, gypsum, and limestone.
Pag112
Published Research Articles in International Journals 2014-2015
Journal of the chemical Society of Pakistan 2014
Hydantoin in Heterocyclic Synthesis: Synthesis of new Imidazopyridine, Iimidazotriazole, Pyrazolopurinone, Pyranoimidazole, Imidazopyridazine and Imidazopyrazole Derivatives
Ibrahim. S. Abdel Hafiz,aMohamed. A. M. Abdel Reheim,a Mahmoud. M. M. Ramiz,b aDepartment of Chemistry, Faculty of Science, Suez Canal University, Arish, Egypt bFaculty of Electronic Engineering, Minufiya University, Menouf, Egypt
Abstract
The chemistry and properties of hydantoins and their derivatives have been investigated for more than 140 years. The hydantoin moiety which is present in various biologically active compounds represents a pharmaceutical importance [1, 2]. They have a wide biological activities such as integrins and kinases inhibitors, [3, 4], anti-convulsants, anti- epileptics [5], fungicides, herbicides [6] anti-bacterial, anti-mycobacterial [7] and potent activity against the herpes simplex virus (HSV) [8], human immune deficiency virus (HIV) [9] and the leukemia subpanel [10]. Hydantoin derivatives can also be found as antiarrhythmics (azimilide), antimicrobial agents (nitrofurantoin), skeletal muscle relaxants (dantrolene) and nonsteroidal antiandrogens (nilutamide), while allantoin is used as a keratolytic, astringent, wound remedy, antacid and antipsoriatic drug [11]. Hydantoins also exhibit antidepressant, antiviral and antithrombotic activities, as well as inhibitory activity against some enzymes (human aldose reductase and human leucocyte elastase [12]. Finally, some herbicides (spirohydantoin, thioxohydantocidin), fungicides (clodantoin) and insecticides also have the hydantoin skeleton in their structure [13, 14]. In the chemical industry various 5,5-disubstituted hydantoins constitute the basis of a new generation of weatherproof high-temperature stable epoxy resins [15]. Furthermore, hydantoin and hydantoin derivatives were used as a monomer for the synthesis of condensation polymers [16, 17]. This led us to prepare a series of the novel structural analogues of these lead compounds. Owing to the above facts and as continuation of our program of identification of new active leads that may be valuable in designing new agents [18, 19], the present work reports the synthesis of new heterocyclic compounds.
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Published Research Articles in International Journals 2014-2015
HETEROCYCLES 2015 (91)134 - 148
SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL ACTIVITY OFSOME NOVEL COUMARIN DERIVATIVES CONTAINING PYRIDINEMOIETY
Ahmed A. Fadda,1* Khaled S. Mohamed,2 Hala M. Refat,3 and Engy E. El-Bialy1 1Department of Chemistry, Faculty of Science, Mansoura University, ET-35516 Mansoura, Egypt 2Engineering Chemistry Department, Higher Institute for Engineering and Technology, New Damietta, Egypt; 3Department of Chemistry, Faculty of Education, Suez Canal University, 45511 Al-Arish, Egypt
Abstract The 2-acetyl-3H-benzo[f]chromen-3-one (1) was used as a key intermediate for the synthesis of 3-(1-amino-3-oxo-3H-benzo[f]chromen-2-yl)-but-2-enenitrile derivatives 3a-d via condensation reactions with activated nitrile derivatives in the presence of ammonium acetate. Moreover, the 3a- d underwent interamolecular cyclization to form 3-alkyl-2-amino- 4-methyl-5- oxo-5H-benzo[5,6]chromeno[4,3-b]pyridine 4a-d. Compound 1 reacts with acetophenone and cyclohexanone in the presence of cyanoacetamide to afford the benzo[5,6]chromeno[3,4-c]pyridin-5-one derivatives 5 and 6, respectively. Also, 4-aryl-6- [benzo[f]coumarin-3-yl]-3-cyano-2-pyridone derivatives 8a-d were synthesized by an efficient and convenient method by the one-pot reaction of 1 with aromatic aldehydes 7a-d and malononitrile in the presence of sodium hydroxide under a solvent free condition. This method has the advantages of mild reaction conditions, easy workup and inexpensive reagents. Moreover, 2-(4,6- diphenylpyridin-2-yl)-3H-benazo[f]chromen-3-one (16) was prepared via reaction of α-pyrdinium salt of methyl ketone of 1 with benzalacetophenone in the presence of ammonium acetate. The structures of the new synthesized compounds were confirmed by spectral data and elemental analyses. New compounds were tested for in vitro cytotoxicity against heptacelluar carcinoma (HepG2) and breast cancer (MCF-7) in addition to their antibacterial evaluation.
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Published Research Articles in International Journals 2014-2015
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Published Research Articles in International Journals 2014-2015
Nanotoxicology 2015 1-6
Sorption of silver nanoparticles to laboratory plastic during (eco)toxicological testing
Anzhela Malysheva1*, Angela Ivask2*, Claudia Hager2, Gianluca Brunetti1, Ezzat R. Marzouk1,3, Enzo Lombi1, and Nicolas H. Voelcker2
1Centre for Environmental Risk Assessment and Remediation, University of South Australia, Mawson Lakes, Australia, 2Mawson Institute, Universityof South Australia, Mawson Lakes, Australia, and 3Division of Soil and Water Sciences, Faculty of Environmental Agricultural Sciences, Suez CanalUniversity, North Sinai, Egypt
Abstract Here, we evaluate the extent of sorption of silver nanoparticles (AgNPs) with different primarysizes (30 and 70 nm) and surface properties (branched polyethylene imine, „„bPEI‟‟ and citrate coating) to laboratory plastic during (eco) toxicological testing. Under conditions of algal growth inhibition assay, up to 97% of the added AgNPs were sorbed onto the test vessels whereas under conditions of in vitro toxicological assay with mammalian cells, the maximum loss of AgNPs was 15%. We propose that the high concentration of proteins and biomolecules in the in vitro toxicological assay originating from serum-containing cell culture medium prevented NP sorption due to steric stabilisation. The sorption of AgNPs to test vessels was clearly concentration dependent. In the conditions of algal growth inhibition assay at 10 ng AgNPs/mL, up to 97% of AgNPs were lost from the test while at higher concentrations (1000 ng AgNPs/mL), the loss of AgNPs was remarkably smaller, up to 64%. Sorption of positively charged bPEI-coated AgNPs was more extensive than the sorption of negatively charged citrate-coated AgNPs and, when calculated on a mass basis, more 70 nm-sized Ag than 30 nm Ag sorbed to plastic surfaces. In summary, this study demonstrates that the loss of AgNPs during (eco)toxicological tests due to sorption on test vessel surfaces is significant, especially in diluted media (e.g. in algal growth medium) and at low NP concentrations. Thus, to ensure the accurate interpretation of (eco)toxicological results, the loss of AgNPs due to adsorption to test vessels should not be overlooked and considered for each specific case.
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Published Research Articles in International Journals 2014-2015
Egyptian Journal of Biological Pest Control 2015 (2)407-413
Biological control and induction of systemic resistance against cucumber Fusarium wilt by plant growth promoting rhizo-organisms
El-Sharkawy, E.E.S. 1, Abdalla, M.Y.2 and El-Shemy, A.O.1
1 Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt 2 Faculty of Environmental Agricultural Sciences at Al-Arish, Suez Canal University, Egypt
Abstract Cucumber Fusarium wilt, caused by Fusarium oxysporum f. sp. cucumerinum (FOC), is one of the most important cucumber diseases in Egypt. The effects of five plant growth promoting rhizo-organisms (PGPRs) namely Pseudomonas fluorescens, Bacillus subtilis, Rhizobium sp., Trichoderma harzianum and T. viride on the linear growth of FOC, controlling Fusarium wilt incidence and inducing systemic resistance were investigated under laboratory and glasshouse conditions using Al-Zaeem cucumber cultivar as a susceptible cultivar and Hayel as a resistant one. Results indicated that P. fluorescens led to the greatest reduction in FOC mycelial growth (87.04%), followed by T. viride and T. harzianum at 83.33 and 80.74%, respectively. The tested PGPRs significantly reduced wilt disease incidence in cucumber plants as compared with control treatment (soil infested only with FOC) in both susceptible and resistant cultivars. The greatest percentages of healthy survived plants and disease reduction were observed in P. fluorescens, T. viride and T. harzianum treatments. The tested PGPRs also increased plant height, root length, leaves number and fresh weight in both studied cultivars as compared with control treatment. Moreover, phenol content, peroxidase (PO) and polyphenol oxidase (PPO) activities in PGPR treated plants were significantly higher than those in control plants either in susceptible or resistant cultivar. Also, phenol contents and the activities of oxidative enzymes were higher in cucumber plants grown in FOC-infested soil than in those grown in non-infested controls irrespective of PGPR treatments.
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Published Research Articles in International Journals 2014-2015
Egyptian Journal of Biological Pest Control 2015 (2)283-288
Survey of Aphid Species and their Associated Parasitoids and Predators on Wild Plants in North Sinai Governorate, Egypt.
Aziza, M. EL-Gantiry; Ahmed, S. A.; El-Bassioni, M. N.; Mahfouz, H. M.; El-Deeb, M. G. A.
Plant Production Department, Faculty of Environmental Agricultural Sciences at Al-Arish, Suez Canal University, Egypt
Abstract A survey of the most abundant aphid species (Homoptera: Aphididae) and their associated parasitoids and predators found on 16 wild plants was conducted in the region of El-Arish, El-Sheikh Zewaid and Rafah in North Sinai, Egypt in two successive years 2011 and 2012. Fourteen aphid species were identified, 7 were recorded for the first time, Dysaphisfoeniculus (Theobald), Aphis compositaeTheoblad, Brachycaudushelichrysi (Kaltenbach), A. spiraecola Patch, Macrosiphumrosae (L.), Toxopteraodinae (Van der Goot) and Maculolachnussubmacula (Walker). Also, Myzuspersicae (Sulzer), A. fabaeScop., B. amygdalinus (Schouteden), A. gossypii Glover., Brevicorynebrassicae (L.), A. craccivora Koch and A. nerii Boyer. Associated parasitoid and predatory species were; 5 primary hymenopterous parasitoid species: AphidiusmatricariaeHaliday, Binodoxysanagelicae (Haliday), EphedruspersicaeFroggatt, Lysiphlebus confuses Tremblay et Eady, L. fabarum (Marshall), 2 hyperparasitoid species: Syrphophagus sp. and Alloxysta sp. and 6 predatory species: Coccinellaundecimpunctata L., C. septempunctataL., HippodamaconvergensGuérin-Méneville, Scymnusinterruptus (Goeze), Chrysoperlacarnea (Steph.) and SyrphuscorollaeFabricius. Occurrence periods, locations, infested plant parts and abundance rates of the surveyed species were recorded.
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Published Research Articles in International Journals 2014-2015
PREPARED BY "Tghreed Mohamed Ahmed" "Doaa Mohamed FaragKhalaf"
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