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Genetic Diversity and Population Structure of Pummelo (Citrus Maxima) Germplasm in China
Tree Genetics & Genomes (2017) 13: 58 DOI 10.1007/s11295-017-1133-0 ORIGINAL ARTICLE Genetic diversity and population structure of pummelo (Citrus maxima) germplasm in China Huiwen Yu1 & Xiaoming Yang 1 & Fei Guo1 & Xiaolin Jiang1 & Xiuxin Deng1 & Qiang Xu1 Received: 31 July 2016 /Revised: 11 March 2017 /Accepted: 19 March 2017 /Published online: 26 April 2017 # Springer-Verlag Berlin Heidelberg 2017 Abstract Pummelo (Citrus maxima) is one of the basic spe- Keywords Pummelo . Genetic diversity . Population cies of Citrus. It has been cultivated for about 4000 years in structure . Nuclear simple sequence repeat (nSSR) China, and therefore, there are abundant germplasm during the long time of culture. However, there is still a lack of a detailed study of the genetic characteristics of pummelo pop- Introduction ulation. In this study, genetic diversity and population struc- ture among 274 pummelo accessions collected in China were Citrus is one of the most important fruit crops in the world. analyzed using 31 nuclear simple sequence repeat (nSSR) The genetic background of citrus is very complicated because markers. The observed heterozygosity was calculated as of its biological characteristics, such as wide sexual compati- 0.325 and genetic differentiation Fst as 0.077. Genetic struc- bility on interspecies and intergenus levels. The complex ge- ture analysis divided the whole germplasm into three subpop- netic background has hindered the genetic studies in citrus. ulations, Pop-a, Pop-b, and Pop-c. Pop-a was composed of Exploring genetic variation within a single species will facil- accessions mostly from Southeast China, Pop-b was com- itate genetic analysis such as genome-wide association studies posed of accessions from the central region of South China, (GWAS) of important traits. -
Reaction of Tangerines Genotypes to Elsinoe Fawcettiiunder
Reaction of tangerines genotypes to Elsinoe fawcettii under natural infection conditions Crop Breeding and Applied Biotechnology 11: 77-81, 2011 Brazilian Society of Plant Breeding. Printed in Brazil Reaction of tangerines genotypes to Elsinoe fawcettii under natural infection conditions Marcelo Claro de Souza1*, Eduardo Sanches Stuchi2 and Antonio de Goes3 Received 11 February 2010 Accepted 30 September 2010 ABSTRACT - A citrus scab disease, caused by Elsinoe fawcettii, is currently found in all citrus areas throughout Brazil. That being, given the importance of this casual agent, the behavior of tangerines and hybrids influenced by this pathogen was evaluated under natural infection conditions. This study was performed with plants around 15 years old without irrigation; 100 fruits of three plants were collected during harvest season, using a grade scale varying from 0 (absence of symptoms) to 6 (severe symptoms) the level of disease severity was determined. Among the cultivars, citrus scab resistance was observed in Citrus deliciosa, C. tangerina, C. nobilis; a mandarin hybrid (C. nobilis x C. deliciosa) and a satsuma hybrid (C. unshiu x C. sinensis). Among the other genotypes, symptoms were observed with levels of severity ranging from 1 to 3, indicating moderate resistance. Key words: Citrus scab, citrus crop, resistant varieties. INTRODUCTION In Brazil, E. fawcettii is responsible for citrus scab. The disease is widespread in many humid, citrus-cultivating In many citrus production areas around the world, areas around the world and decreases fruit values on the Elsinoe fawcettii is one of the main fungi diseases found. fresh-fruit market (Feichtenberger et al. 1986). In young It attacks a wide variety of citrus species and cultivars, plants or under severe infection, it may cause significant resulting in scab disease on leaves, twigs, and fruits (Timmer fruit drop. -
Canker and Greening – Lessons from South America by Bob Rouse and Fritz Roka
Citrus Expo follow-up Canker and greening – lessons from South America By Bob Rouse and Fritz Roka he 2006 Citrus Expo featured citrus managers from Cecil Taylor described citrus production in the northeast Brazil and Argentina who spoke about their manage - region of Argentina where producers grow common oranges, ment programs to control citrus canker and citrus mandarins and grapefruit. Tgreening diseases. The strategies they described Argentina was devastated by tristeza in the 1940s and in have been successful and hopefully transferable to citrus the 1960s began to see canker. Initial control efforts included production in Florida. eradication, tree defoliation and spraying enough copper that The Florida canker eradication program was abandoned in trees turned blue. These efforts proved fruitless, and growers the spring of 2006 after the USDA predicted that canker demanded an end to any eradication policy. would spread by 100,000 acres before the end of the year. During these early years, windbreaks were not part of any Currently, most of the canker is south of Polk County’s State perceived solution. By the end of the 1980s, they began to re - Road 60. Since the eradication program ended, there have alize, however, that the key to canker control lies in slowing been about 100 new finds monthly. down the wind, even though they did not experience strong Grower self-inspections have accounted for 40 percent of winds. They started with low-growing plants like sugarcane, the new finds. DPI inspectors have identified the remaining maze and sunflower. By the 1990s, they saw the need for 60 percent of new canker finds. -
Rangpur Lime X Troyer Citrange, a Hybrid Citrus Rootstock for Closely Spaced Trees
Proc. Fla. State Hort. Soc. 99:33-35. 1986. RANGPUR LIME X TROYER CITRANGE, A HYBRID CITRUS ROOTSTOCK FOR CLOSELY SPACED TREES W. S. Castle A combination of diseases, repeated freezes, and other University of Florida, IFAS factors has reemphasized the importance of rootstocks in Citrus Research and Education Center Florida. Moreover, the effects of these factors illustrate the 700 Experiment Station Road inherent weaknesses in virtually all citrus rootstocks and Lake Alfred, FL 33850 the need to continually search for new, improved ones. Another recent trend related to rootstocks has been C. O. YOUTSEY the shift toward more closely spaced trees, particularly FDACS, Division of Plant Industry within the row (7,8). Rootstocks well-suited for dense plan Citrus Budwood Registration Bureau tings have not been available although such stocks are 3027 Lake Alfred Road being evaluated and one appears particularly promising Winter Haven, FL 33881 (1, 3, 4, 8). It is a hybrid of Rangpur lime and Troyer D. J. Hutchison citrange (RxT) and has been under study in Florida for 18 United States Department of Agriculture yr. During this period, trees on RxT have demonstrated Agricultural Research Service sufficient commercial potential to justify our presentation 2120 Camden Road in this report of their performance and a description of Orlando, FL 32803 RxT and its characteristics. Additional index words. Blight, tristeza, tree size control. History Dr. J. R. Furr, formerly a plant breeder with the U.S. Abstract. A hybrid of Rangpur lime (Citrus limonia Osb.) and Department of Agriculture (USDA) at Indio, California, Troyer citrange [ C. sinensis (L) Osb. -
Known Host Plants of Huanglongbing (HLB) and Asian Citrus Psyllid
Known Host Plants of Huanglongbing (HLB) and Asian Citrus Psyllid Diaphorina Liberibacter citri Plant Name asiaticus Citrus Huanglongbing Psyllid Aegle marmelos (L.) Corr. Serr.: bael, Bengal quince, golden apple, bela, milva X Aeglopsis chevalieri Swingle: Chevalier’s aeglopsis X X Afraegle gabonensis (Swingle) Engl.: Gabon powder-flask X Afraegle paniculata (Schum.) Engl.: Nigerian powder- flask X Atalantia missionis (Wall. ex Wight) Oliv.: see Pamburus missionis X X Atalantia monophylla (L.) Corr.: Indian atalantia X Balsamocitrus dawei Stapf: Uganda powder- flask X X Burkillanthus malaccensis (Ridl.) Swingle: Malay ghost-lime X Calodendrum capense Thunb.: Cape chestnut X × Citroncirus webberi J. Ingram & H. E. Moore: citrange X Citropsis gilletiana Swingle & M. Kellerman: Gillet’s cherry-orange X Citropsis schweinfurthii (Engl.) Swingle & Kellerm.: African cherry- orange X Citrus amblycarpa (Hassk.) Ochse: djerook leemo, djeruk-limau X Citrus aurantiifolia (Christm.) Swingle: lime, Key lime, Persian lime, lima, limón agrio, limón ceutí, lima mejicana, limero X X Citrus aurantium L.: sour orange, Seville orange, bigarde, marmalade orange, naranja agria, naranja amarga X Citrus depressa Hayata: shiikuwasha, shekwasha, sequasse X Citrus grandis (L.) Osbeck: see Citrus maxima X Citrus hassaku hort. ex Tanaka: hassaku orange X Citrus hystrix DC.: Mauritius papeda, Kaffir lime X X Citrus ichangensis Swingle: Ichang papeda X Citrus jambhiri Lushington: rough lemon, jambhiri-orange, limón rugoso, rugoso X X Citrus junos Sieb. ex Tanaka: xiang -
Tropical Horticulture: Lecture 32 1
Tropical Horticulture: Lecture 32 Lecture 32 Citrus Citrus: Citrus spp., Rutaceae Citrus are subtropical, evergreen plants originating in southeast Asia and the Malay archipelago but the precise origins are obscure. There are about 1600 species in the subfamily Aurantioideae. The tribe Citreae has 13 genera, most of which are graft and cross compatible with the genus Citrus. There are some tropical species (pomelo). All Citrus combined are the most important fruit crop next to grape. 1 Tropical Horticulture: Lecture 32 The common features are a superior ovary on a raised disc, transparent (pellucid) dots on leaves, and the presence of aromatic oils in leaves and fruits. Citrus has increased in importance in the United States with the development of frozen concentrate which is much superior to canned citrus juice. Per-capita consumption in the US is extremely high. Citrus mitis (calamondin), a miniature orange, is widely grown as an ornamental house pot plant. History Citrus is first mentioned in Chinese literature in 2200 BCE. First citrus in Europe seems to have been the citron, a fruit which has religious significance in Jewish festivals. Mentioned in 310 BCE by Theophrastus. Lemons and limes and sour orange may have been mutations of the citron. The Romans grew sour orange and lemons in 50–100 CE; the first mention of sweet orange in Europe was made in 1400. Columbus brought citrus on his second voyage in 1493 and the first plantation started in Haiti. In 1565 the first citrus was brought to the US in Saint Augustine. 2 Tropical Horticulture: Lecture 32 Taxonomy Citrus classification based on morphology of mature fruit (e.g. -
Classification and Cultivars
1 Classification and Cultivars 2 Two Tribes • Clauseneae • Citreae has 3 Subtribes –Triphasiinae –Balsamocitrineae –Citrinae 3 Fortunella • Four species - Small trees and shrubs. • Flowers later than Citrus. • Freeze - hardy • Small fruit –‘Meiwa’ and ‘Marumi’ - round –‘Nagami’ ovate 4 Poncirus • Two trifoliate spp. –trifoliata ‘Flying Dragon’ –poyandra • Deciduous • Thorny, Cold hardy, long thorns • Makes great hedges , rootstocks 5 Microcitrus • Northeastern rainforest Australia • Moderate-sized trees. • Leaves are unifoliate dimorphic • Microcitrus australasica –Resistant to burrowing nematode and phytophthora • Micro leaves, flowers, and fruit 6 Clymenia • Unifoliate acuminate leaves tapering into very short petiole. • Branches are thornless. • Style shorter than other true Citrus and stigma is larger and flattened • Fruit - ovoid, thin peeled, many oil glands, many small seeds. 7 Eremocitrus • Xerophytic native of Australia • Spreading long drooping branches • Leaves unifoliate, greyish green, thick, leatherly, and lanceolate. • Sunken stomata, freeze hardy • Ideal xeroscape plant. 8 Citrus - Subgenus Eucitrus • Vesicles - no acrid or bitter oil • C. medica (Citrons) –Uses - candied peel, • Jewish ceremony • Exocortis indicator 9 Citrus limon (Lemons) • Commerce –‘Lisbon’ and ‘Eureka’ • Dooryard –Meyer (Lemon hybrid) • Rough Lemon –Rootstock 10 Lemon Hybrids • Lemonage (lemon x sweet orange) • Lemonime (lemon x lime) • Lemandrin (lemon x mandarin) • Eremolemon (Eremocitrus x lemon) - Australian Desert Lemon 11 Citrus aurantifolia (Limes) • ‘Key’ or ‘Mexican’ limes • ‘Tahiti’ or ‘Persian’ limes some are triploids and seedless • C. macrophylla (lime-like fruit) –Rootstock in California • Lemonimes (lime x lemon) • Limequats (lime x kumquat) 12 • Not grown either in Tahiti or Persian (Iran) • Seedless and marketed when still dark green 13 C. aurantium - Sour Orange • ‘Seville’ in Southern Europe –Orange marmalade • ‘Bouquet’ & ‘Bergamot’ • - Italy –Essential oil • Many forms like ‘Bittersweet’ –Rootstock - High quality fruit. -
Rangpur Lime Disease and Its Relatiomship to Exocortis
RANGPUR LIME DISEASE AND ITS RELATIOMSHIP TO EXOCORTIS Instituto Agronomico, Campinas, Sgo Paulo, Brazil INTRODUCTION The Rangpur lime has been used in Brazil more or less extensively as a rootstock for several orange varieties since the early citrus plantings were established. When the tristeza disease invaded and destroyed those Brazilian citrus groves which were budded on sour orange- and sweet lime (lima da Persia), it was demonstrated (5)*, that the Rangpur lime was tolerant to tristeza virus. Since then, nurserymen and growers have favored its use as rootstock for many kinds of citrus plants. However, the young plant- ing~soon showed symptoms of a "new disease" that seemed to be related to the Rangpur lime rootstock. The behavior of Poncirus trifoliata and Rana~ur-1 lime as rootstocks for some sweet orange, grapefruit, and mandarin clones in the tests at the Limeira, RibeirSo Preto, and Tiet6 Stations gave us evidence that the "new disease" was caused by the exocortis virus (6).Olson and Shull (9),in Texas, and Reitz and Knorr (lo), in Florida, were able to confirm that thesis. Olson and Shull demonstrated also that Rangpur l&e shows xyloporosis symptoms when as a rootstock it has been budded with infected scion varieties. Grant et al. (3) reported later that they found xyloporosis symptoms on Rangpur- - lime in Brazil. This paper reports on additional studies made in Brazil on transmission of exocortis virus, symptomat~log~of affected trees, and the effects of the disease on fruit produc- tion. MATERIALS AND RESULTS Incubation Period in Young Trees. One hundred Rangpur lime seedlings were budded in the nursery in May, 1950, with clonal buds from the following varieties (20 each) : Bahianinha, Bargo, and Pera oranges, Marsh seedless grapefruit, and Eureka lemon. -
Citrus from Seed?
Which citrus fruits will come true to type Orogrande, Tomatera, Fina, Nour, Hernandina, Clementard.) from seed? Ellendale Tom McClendon writes in Hardy Citrus Encore for the South East: Fortune Fremont (50% monoembryonic) “Most common citrus such as oranges, Temple grapefruit, lemons and most mandarins Ugli Umatilla are polyembryonic and will come true to Wilking type. Because most citrus have this trait, Highly polyembryonic citrus types : will mostly hybridization can be very difficult to produce nucellar polyembryonic seeds that will grow true to type. achieve…. This unique characteristic Citrus × aurantiifolia Mexican lime (Key lime, West allows amateurs to grow citrus from seed, Indian lime) something you can’t do with, say, Citrus × insitorum (×Citroncirus webberii) Citranges, such as Rusk, Troyer etc. apples.” [12*] Citrus × jambhiri ‘Rough lemon’, ‘Rangpur’ lime, ‘Otaheite’ lime Monoembryonic (don’t come true) Citrus × limettioides Palestine lime (Indian sweet lime) Citrus × microcarpa ‘Calamondin’ Meyer Lemon Citrus × paradisi Grapefruit (Marsh, Star Ruby, Nagami Kumquat Redblush, Chironja, Smooth Flat Seville) Marumi Kumquat Citrus × sinensis Sweet oranges (Blonde, navel and Pummelos blood oranges) Temple Tangor Citrus amblycarpa 'Nasnaran' mandarin Clementine Mandarin Citrus depressa ‘Shekwasha’ mandarin Citrus karna ‘Karna’, ‘Khatta’ Poncirus Trifoliata Citrus kinokuni ‘Kishu mandarin’ Citrus lycopersicaeformis ‘Kokni’ or ‘Monkey mandarin’ Polyembryonic (come true) Citrus macrophylla ‘Alemow’ Most Oranges Citrus reshni ‘Cleopatra’ mandarin Changshou Kumquat Citrus sunki (Citrus reticulata var. austera) Sour mandarin Meiwa Kumquat (mostly polyembryonic) Citrus trifoliata (Poncirus trifoliata) Trifoliate orange Most Satsumas and Tangerines The following mandarin varieties are polyembryonic: Most Lemons Dancy Most Limes Emperor Grapefruits Empress Tangelos Fairchild Kinnow Highly monoembryonic citrus types: Mediterranean (Avana, Tardivo di Ciaculli) Will produce zygotic monoembryonic seeds that will not Naartje come true to type. -
Known Host Plants of Huanglongbing (HLB) and Asian Citrus Psyllid
Known Host Plants of Huanglongbing (HLB) and Asian Citrus Psyllid Diaphorina Liberibacter citri Plant Name asiaticus Citrus Huanglongbing Psyllid Aegle marmelos (L.) Corr. Serr.: bael, Bengal quince, golden apple, bela, milva X Aeglopsis chevalieri Swingle: Chevalier’s aeglopsis X X Afraegle gabonensis (Swingle) Engl.: Gabon powder-flask X Afraegle paniculata (Schum.) Engl.: Nigerian powder- flask X Artocarpus heterophyllus Lam.: jackfruit, jack, jaca, árbol del pan, jaqueiro X Atalantia missionis (Wall. ex Wight) Oliv.: see Pamburus missionis X X Atalantia monophylla (L.) Corr.: Indian atalantia X Balsamocitrus dawei Stapf: Uganda powder- flask X X Burkillanthus malaccensis (Ridl.) Swingle: Malay ghost-lime X Calodendrum capense Thunb.: Cape chestnut X × Citroncirus webberi J. Ingram & H. E. Moore: citrange X Citropsis gilletiana Swingle & M. Kellerman: Gillet’s cherry-orange X Citropsis schweinfurthii (Engl.) Swingle & Kellerm.: African cherry- orange X Citrus amblycarpa (Hassk.) Ochse: djerook leemo, djeruk-limau X Citrus aurantiifolia (Christm.) Swingle: lime, Key lime, Persian lime, lima, limón agrio, limón ceutí, lima mejicana, limero X X Citrus aurantium L.: sour orange, Seville orange, bigarde, marmalade orange, naranja agria, naranja amarga X Citrus depressa Hayata: shiikuwasha, shekwasha, sequasse X Citrus grandis (L.) Osbeck: see Citrus maxima X Citrus hassaku hort. ex Tanaka: hassaku orange X Citrus hystrix DC.: Mauritius papeda, Kaffir lime X X Citrus ichangensis Swingle: Ichang papeda X Citrus jambhiri Lushington: rough lemon, jambhiri-orange, limón rugoso, rugoso X X Citrus junos Sieb. ex Tanaka: xiang cheng, yuzu X Citrus kabuchi hort. ex Tanaka: this is not a published name; could they mean Citrus kinokuni hort. ex Tanaka, kishu mikan? X Citrus limon (L.) Burm. -
Caribbean Fruit Fly Host List
1 Caribbean Fruit Fly Host List Common Name Botanical Name Akee Blighia sapida Allspice Pimenta dioica Ambarella Spondias cytherea Atemoya Annona cherimola X A. squamosa Apple Malus sylvestris, Malus domestica Malus spp. Autumn Maple Tree Bischofia javanica Avocado, except commercial fruit Persea americana Balsam Apple Momordica balsamina Barbados Cherry Malpighia glabra Bell Pepper, except commercial fruit Capsicum frutescens, Capsicum annuum Birchberry Eugenia ligustrina Blackberry Rubus hybrid Box Orange Severinia buxifolia Brazil Cherry Eugenia dombeyi Cabeluda Plinia glomerata Calabur Muntingia calabura Calamondin X Citrofortunella mitis Carambola Averrhoa carambola Ceylon Gooseberry Dovyalis hebecarpa Cherry of the Rio Grande Eugenia aggregata Clementine Citrus reticulata Cocoplum Chrysolbalanus icaco Custard Apple, Sugar Apple Annona squamosa, Annona reticulata Egg Fruit Pouteria campechiana Date Palm Phoenix dactylifera Fig Ficus carica Garcinia aristata Garcinia aristata Garcinia Garcinia spp. Governor's Plum Flacourtia indica Grapefruit Citrus paradisi 2 Caribbean Fruit Fly Host List Grumichama Eugenia brasiliensis Guava (all) Psidium spp. Guiana Plum Drypetes lateriflora Hog Plum Spondias mombin Imbe Garcinia livingstonei Jaboticaba Myrciaria cauliflora Jack Orangequat Citrus nobilis 'unshu' x Fortunella sp. Jambolan Plum Syzygium cumini Jamboisier Rouge Eugenia pyriformis Cambess. var. uvalha Japanese Pear Pyrus pyrifolia Japanese Persimmon Diospyros kaki Java Apple Syzygium samarangense Kei Apple Dovyalis caffra Kieffer Pear -
Improvement of Subtropical Fruit Crops: Citrus
IMPROVEMENT OF SUBTROPICAL FRUIT CROPS: CITRUS HAMILTON P. ÏRAUB, Senior Iloriiciilturist T. RALPH ROBCNSON, Senior Physiolo- gist Division of Frnil and Vegetable Crops and Diseases, Bureau of Plant Tndusiry MORE than half of the 13 fruit crops known to have been cultivated longer than 4,000 years,according to the researches of DeCandolle (7)\ are tropical and subtropical fruits—mango, oliv^e, fig, date, banana, jujube, and pomegranate. The citrus fruits as a group, the lychee, and the persimmon have been cultivated for thousands of years in the Orient; the avocado and papaya were important food crops in the American Tropics and subtropics long before the discovery of the New World. Other types, such as the pineapple, granadilla, cherimoya, jaboticaba, etc., are of more recent introduction, and some of these have not received the attention of the plant breeder to any appreciable extent. Through the centuries preceding recorded history and up to recent times, progress in the improvement of most subtropical fruits was accomplished by the trial-error method, which is crude and usually expensive if measured by modern standards. With the general accept- ance of the Mendelian principles of heredity—unit characters, domi- nance, and segregation—early in the twentieth century a starting point was provided for the development of a truly modern science of genetics. In this article it is the purpose to consider how subtropical citrus fruit crops have been improved, are now being improved, or are likel3^ to be improved by scientific breeding. Each of the more important crops will be considered more or less in detail.