DOCSLIB.ORG

ESV1) Protein and Its Homologue Like- Early Starvation 1 (LESV) During Starch Degradation

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
ESV1) Protein and Its Homologue Like- Early Starvation 1 (LESV) During Starch Degradation Biochemical studies to determine the role of Early Starvation 1 (ESV1) protein and its homologue Like- Early Starvation 1 (LESV) during starch degradation Dissertation zur Erlangung des akademischen Grades "doctor rerum naturalium" (Dr. rer. nat.) in der Wissenschaftsdisziplin Biochemistry eingereicht an der Mathematisch-Naturwissenschaftlichen Fakultät Institut für Biology/Biochemistry der Universität Potsdam von Shadha Abduljaleel AL-Rawi Potsdam. 2020 Main supervisor: Prof Dr. habil. Joerg Fettke Supervisors: Prof. Dr. Alisdair Fernie Reviewers: Prof. Dr. Christophe D’Hulst Prof. Dr. Oluwatoyin A. Odeku Published online on the Publication Server of the University of Potsdam: https://doi.org/10.25932/publishup- 48395 https://nbn-resolving.org/urn:nbn:de:kobv:517-opus4- 483956 Table of contents Table of contents Table of contents ................................................................................................................ 1 Abstract ............................................................................................................................... 5 Zusammenfassung ............................................................................................................. 7 Acknowledgement .............................................................................................................. 9 List of Figures .................................................................................................................. 11 List of Tables .................................................................................................................... 13 Abbreviation ..................................................................................................................... 14 1. Introduction .................................................................................................................. 18 1.1 Carbohydrates ........................................................................................................... 18 1.2 Classification of carbohydrates .................................................................................. 18 1.2.1 Monosaccharides ........................................................................................................................ 18 1.2.2 Disaccharides .............................................................................................................................. 19 1.2.3 Oligosaccharides ......................................................................................................................... 20 1.2.4 Polysaccharides ........................................................................................................................... 21 1.3 Starch and glycogen are the two important storage biopolymers in nature ............... 22 1.3.1 Glycogen ..................................................................................................................................... 22 1.3.2 Starch .......................................................................................................................................... 23 1.4 Starch synthesis ......................................................................................................... 25 1.4.1 Adenosine 5, diphosphate glucose pyrophosphorylase (AGPase) E.C. 2.7.7.27 ........................ 25 1.4.2 Starch Synthases (SSs) E.C. 2.4.1.21 .......................................................................................... 26 1.4.3 Starch branching enzymes (SBEs) E.C. 2.4.1.18 ....................................................................... 27 1.4.4 Starch debranching enzymes DBEs E.C. 3.2.1.41 ..................................................................... 29 1.5 Disproportionating enzyme D-enzyme E.C. 2.4.1.25 .................................................. 29 1.6 Alpha glucan phosphorylase ...................................................................................... 31 1.7 Starch degradation .................................................................................................... 32 1.8 Metabolism of starch ................................................................................................. 41 1.9 The biological function for complex formation of starch metabolic enzymes ............. 42 1.10 Putative protein-protein interaction within starch granules..................................... 44 1.11 Novel proteins influence starch metabolism ............................................................. 45 1.11.1 Regulatory proteins in starch synthesis ..................................................................................... 45 1.11.2 Regulatory proteins in starch degradation................................................................................. 47 1.12 The aims of the study ............................................................................................... 51 2. Materials and Methods ................................................................................................ 53 2.1 Materials ................................................................................................................... 53 1 Table of contents 2.1.1 Plant material and growth conditions.......................................................................................... 53 2.1.2 Starch isolation ........................................................................................................................... 53 2.1.3 Chemicals ................................................................................................................................... 54 2.1.4 Enzymes ...................................................................................................................................... 54 2.2 Methods ..................................................................................................................... 54 2.2.1 Preparation of E.coli LB21 competent cells ............................................................................... 54 2.2.2 Preparation of E.coli DH5 ɑ competent cells .............................................................................. 54 2.2.3 Preparation of agarose gel electrophoresis .................................................................................. 55 2.2.4 Preparation of SDS-PAGE .......................................................................................................... 55 2.2.5 Western blot ................................................................................................................................ 56 2.2.6 Detection of Like-Early Starvation 1 protein LESV in Arabidopsis thaliana ............................ 56 2.2.7 Sequence identification of Like-Early Starvation 1 protein LESV in Arabidopsis thaliana genome................................................................................................................................................. 57 2.2.8 Cloning of Arabidopsis LESV protein ........................................................................................ 58 2.2.9 Agarose-gel electrophoresis ........................................................................................................ 60 2.2.10 DNA-Extraction from agarose gel (GFX PCR DNA and GEL Band Purification Kit) ............ 60 2.2.11 Ligation of DNA fragments ...................................................................................................... 60 2.2.12 Transformation of plasmid DNA in competent E.coli cells ...................................................... 61 2.2.13 Colony PCR .............................................................................................................................. 61 2.2.14 Plasmid preparation (Miniprep) ................................................................................................ 62 2.2.15 Restriction with endonucleases ................................................................................................. 62 2.2.16 Quantification of DNA ............................................................................................................. 63 2.2.17 Sequencing ................................................................................................................................ 63 2.2.18 Subcloning of LESV sequence into pET23B expression vector ............................................... 63 2.2.18.1 Restriction digestion for cutting the target sequence and vector ....................................... 63 2.2.18.2 Ligation of LESV sequence into pET23b expression vector ............................................. 64 2.2.18.3 Minipreparation ................................................................................................................. 65 2.2.19 Cloning of LESV without transit peptide (tLESV) ................................................................... 65 2.2.20 Expression and small scale purification of LESV and tLESV in E.coli BL21 cells ................. 66 2.2.21 Preparation of glycerol stock for E.coli BL21 cells containing the plasmid with the protein of interest ................................................................................................................................................. 67 2.2.22 Expression and purification of recombinant LESV and tLESV (large scale) ........................... 67 2.2.23 Measuring proteins concentration ............................................................................................. 68 2.2.24 Protein
Load more

Recommended publications
  • Archaeological Starch Preservation and Methodological Parameters: Where Does Qaraqara Fit?
    ARCHAEOLOGICAL STARCH PRESERVATION AND METHODOLOGICAL PARAMETERS: WHERE DOES QARAQARA FIT? Thesis Presented in Partial Fulfillment of the Requirements for the Degree of Master of Arts in the Graduate School of the Ohio State University By Nicole Marie Hernandez, B.A. Department of Anthropology The Ohio State University 2015 Master’s Committee: Professor Julie S. Field, Advisor Professor Kristen J. Gremillion Professor Robert Cook Copyright by Nicole Marie Hernandez 2015 ABSTRACT Starch granule analysis is a relatively new methodology that can aid in various areas of archaeological research, including the determination of subsistence patterns and transitions, mobility, tool use, and ecology. This study examines undecorated ceramic fragments recovered from Qaraqara, Fiji for the presence of adhered starch. Although the three starch extraction methods used were not successful, the research process provides an opportunity to explain the lack of success by investigating ideal archaeological starch preservation parameters and extraction methodologies. Many factors contribute to the rarity of preserved starch granules in archaeological settings, including its bio-chemical structure, the decomposition of microorganisms in soil, chemical interactions, and environmental conditions. To determine ideal environmental conditions for starch preservation, successful extraction methods, and the effect of archaeological materials on success rates for extraction, a meta-analysis was conducted on a sample of archaeological studies that also sought to extract preserved starch. Key environmental variables that support the preservation of starch were identified, including a specific range of average temperatures, rainfall amounts, and altitudes. Using chi-square statistical tests, this study determined that an average temperature of 19-22° C significantly contributes to the preservation of archaeological starch.
    [Show full text]
  • Starch and Glycogen Analyses: Methods and Techniques
    biomolecules Review Starch and Glycogen Analyses: Methods and Techniques Henrike Brust 1, Slawomir Orzechowski 2 and Joerg Fettke 3,* 1 Leibniz Institute for Plasma Science and Technology, Institute of Biochemistry and Biology, Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany; [email protected] 2 Department of Biochemistry and Microbiology, Warsaw University of Life Sciences-SGGW, ul. Nowoursynowska 159 Budynek 37 P/12B, 02-776 Warszawa, Poland; [email protected] 3 Biopolymer Analytics, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25 Building 20, 14476 Potsdam-Golm, Germany * Correspondence: [email protected] Received: 13 May 2020; Accepted: 7 July 2020; Published: 9 July 2020 Abstract: For complex carbohydrates, such as glycogen and starch, various analytical methods and techniques exist allowing the detailed characterization of these storage carbohydrates. In this article, we give a brief overview of the most frequently used methods, techniques, and results. Furthermore, we give insights in the isolation, purification, and fragmentation of both starch and glycogen. An overview of the different structural levels of the glucans is given and the corresponding analytical techniques are discussed. Moreover, future perspectives of the analytical needs and the challenges of the currently developing scientific questions are included. Keywords: starch; glycogen; analytics 1. Introduction Today, several techniques for the analysis of complex carbohydrates exist, though a single all-embracing method is lacking. All methods provide information about glycan samples, but along with this knowledge, there is unfortunately also a partial loss of specific information. As an example, very large and complex glycans can be analyzed only following partial fragmentation.
    [Show full text]
  • ESTABLISHMENT of HIGH-THROUGHPUT TECHNIQUES for STUDYING STARCH FUNCTIONALITIES by Miguel Angel Alvarez Gonzales
    ESTABLISHMENT OF HIGH-THROUGHPUT TECHNIQUES FOR STUDYING STARCH FUNCTIONALITIES by Miguel Angel Alvarez Gonzales A Thesis Submitted to the Faculty of Purdue University In Partial Fulfillment of the Requirements for the degree of Master of Science Department of Food Science West Lafayette, Indiana August 2019 2 THE PURDUE UNIVERSITY GRADUATE SCHOOL STATEMENT OF COMMITTEE APPROVAL Dr. Yuan Yao, Chair Department of Food Science Dr. Bruce Hamaker Department of Food Science Dr. Clifford Weil Department of Agronomy Approved by: Dr. Arun K. Bhunia Head of the Graduate Program 3 Dedicated to my family 4 TABLE OF CONTENTS LIST OF TABLES .......................................................................................................................... 8 LIST OF FIGURES ........................................................................................................................ 9 LIST OF ABREVIATIONS ......................................................................................................... 12 ABSTRACT .................................................................................................................................. 14 CHAPTER 1. LITERATURE REVIEW ................................................................................... 16 1.1 Introduction ....................................................................................................................... 16 1.2 Clean label movement ....................................................................................................... 17
    [Show full text]
  • Quantification of Soluble Starch from Fresh Potatoes Using Photopette
    Application Note SOLUBLE STARCH EDUCATIONAL EXPERIMENT QUANTIFICATION OF SOLUBLE STARCH FROM FRESH POTATOES USING PHOTOPETTE P.Y. Yap, A. Jain and D. Trau, Tip Biosystems Pte Ltd, Singapore • Educational experiment using Photopette® to measure soluble starch in potatoes and other plants. • Simple extraction method combined with iodine reagent allows easy starch quantification. • Bench centrifuge OBJECTIVE Reagents: This application note provides an educational experiment to quantify soluble starch in fresh potatoes using the • Iodine Reagent Photopette® hand held photometer at 600 nm wavelength. • Starch reference solution made from soluble starch powder (Sigma, S9765). INTRODUCTION Material: • Fresh Potato Starch is a carbohydrate based energy storage molecule found in plants. There are two types of starch - amylose METHOD (highly soluble in water) and amylopectin (slightly soluble in Before performing experiments please refer to the risk- water) [1]. Both types of starch are made from glucose assessment and refer to the Photopette® User Manual for monomers but with a different linkage. operating and safety precautions [2]. EXPERIMENTAL PROCEDURE Preparation Iodine Reagent: The preparation of the Iodine reagent is made following the CLEAPSS Recipe. The reagent is prepared by adding 3 g of KI to 2.54 g I2 and toping up with water to 100 mL. Subsequently, the stock solution is diluted Figure 1a: Amylose Figure 1b: Amylopectin 10 times and stored in the dark. The presence of starch can be measured by its reaction with iodine. Starch and iodine form a dark-blue complex with an Starch Reference Solution: A 0.1% starch reference solution absorbance maximum at 600 nm [1]. is made using soluble starch powder (Sigma, S9765).
    [Show full text]
  • Starch Analysis of HLB-Affected and Control Healthy Citrus Leaves Reveal Variations in the Amylose/ Amylopectin Ratio
    Proc. Fla. State Hort. Soc. 124:69–75. 2011. Starch Analysis of HLB-affected and Control Healthy Citrus Leaves Reveal Variations in the Amylose/ Amylopectin Ratio Pedro Gonzalez, Jose reyes, and ed etxeberria* University of Florida, IFAS, Department of Plant Pathology, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850 AdditionAl index words. Citrus sp., plant disease, phloem disease, starch properties, starch accumulation Leaves of HLB (Huanglongbing or citrus greening)-affected branches contain considerably higher levels of starch than those of HLB-unaffected or “healthy trees.” Over-accumulation of starch takes place in photosynthetic cells, vascular parenchyma, ray parenchyma, and even sieve elements. These observations imply strong disturbances in starch me- tabolism and photoassimilate partitioning brought about by HLB. The elevated starch content, appearance of starch granules in phloem elements, and previous reports of the pathogen effect on starch properties lead us to hypothesize that starch from HLB-affected citrus differs morphologically, physically, and/or chemically from starch accumulated in otherwise healthy leaves. We investigated starch morphology using brightfield, polarized light and SEM and found no morphological differences between HLB-induced starch and that from healthy girdled trees. When reacted with 2% I2, whole starch fractions also showed no significant difference in the absorption spectra (λmax for HLB = 604.1 and girdled 606.2 nm, respectively; n = 6; P ≤ 0.05) nor in their amylose/amylopectin ratio (HLB = 1.4 ± 0.17 and girdled = 1.16 ± 0.07, P ≤ 0.05) after chromatographic separation. Nevertheless, λmax for individual fractions of HLB-affected leaves increased between 11 to 14 nm indicating a significant increase in the degree of polymerization of chain lengths estimated between 12 to 45 glucose units.
    [Show full text]
  • Building a Comparative Starch Reference Collection
    Archaeological science under a microscope: studies in residue and ancient DNA analysis in honour of Thomas H. Loy Lentfer: BUILDING A COMPARatIVE StaRCH REFERENCE COLLECTION FOR INDONESIA AND ITS APPLICatION TO PALAEOENVIRONMEntal AND ARCHAEOLOGICAL RESEARCH 7 BUILDING A COMPARATIVE STARCH REFERENCE COLLECTION FOR INDONESIA AND ITS APPLICATION TO PALAEOENVIRONMENTAL AND ARCHAEOLOGICAL RESEARCH Carol J. Lentfer School of Social Science University of Queensland St. Lucia, QLD 4072 Australia Email: [email protected] ABSTRACT Indonesia has a very long record of hominin occupation involving at least three human species. It also has a rich diversity of plants and a suite of economically important starch-rich staples that include taro (Colocasia esculenta), yams (Dioscorea spp.), bananas (Musa spp.) and sago palms. However very little is known about the prehistory of plant exploitation in the island archipelago. Key archaeological issues that are often discussed and debated, but remain poorly understood, include human adaptations and economic strategies used in different and changing environments, how local economies contributed to the development of plant management systems within the southeast Asian/Pacific regions, and how they might have been influenced by plant management systems initiated and developed elsewhere. Crucial to a burgeoning focus on archaeobotany in island Southeast Asia is the establishment of comparative modern reference collections for the flora of the region. This paper discusses the ‘Indonesian Starch Project’, the relevance of a comparative modern starch reference collection to Indonesian archaeology and the methods and procedures used in its development. The collection is broad-based and focuses on economically and ecologically importat plant groups. To date 121 families and 451genera are represented in the collection.
    [Show full text]
  • STARCH IDENTIFICATION (Microscopy)
    Corn Starch Analysis B-25-1 STARCH IDENTIFICATION (Microscopy) PRINCIPLE Starch granules are suspended in aqueous or nonaqueous media for polarized light microscopy, or are mounted dry on a stub for scanning electron microscopy. The botanical source of the starch is identified according to size and shape of granules, granule surface markings, position of hilum iodine stain color and the presence of maltese crosses under polarized light (Note 1). SCOPE The method is applicable to all starches, but is intended especially for the identification of separated, unmodified, native or commercial starches. SPECIAL APPARATUS 1. Light microscope with bright and polarized light capabilities, equipped with 4X, 10X, 20X, 40X objectives and 8 or 10X wide field eyepieces (Note 2) 2. Precision grade stage and eyepiece micrometers to calibrate microscope magnification (Note 3) 3. Scanning electron microscope with variable KV setting and associated accessories (Note 2) 4. Triode-type sputter coater equipped with gold-palladium target and carbon thread attachment REAGENTS 1. Iodine solution (0.5N): Dissolve 95 g of potassium iodide in 60 mL of purified water, add 32 g iodine crystals, stir until completely in solution, transfer to 500 mL volumetric flask with the aid of purified water, dilute to volume and mix. Analytical Methods of the Member Companies of the Corn Refiners Association, Inc. Accepted 6-20-91 Corn Starch Analysis B-25-2 STARCH IDENTIFICATION (Microscopy) ⎯ continued 2. Iodine solution (0.1 N) : Transfer 100 mL of 0.5 N iodine solution into a 500 mL volumetric flask, dilute to mark with purified water and mix. 3. Buffer solution, pH 5.0 4.
    [Show full text]
  • Starch Grain and Phytolith Evidence for Early Ninth Millennium B.P. Maize
    Starch grain and phytolith evidence for early ninth SEE COMMENTARY millennium B.P. maize from the Central Balsas River Valley, Mexico Dolores R. Pipernoa,b,1, Anthony J. Ranereb,c, Irene Holstb, Jose Iriarted, and Ruth Dickauc aArchaeobiology Program, Department of Anthropology, Smithsonian National Museum of Natural History, Washington, DC 20560 ; bSmithsonian Tropical Research Institute, Apartado Postal 0843-03092, Balboa, Republic of Panama; cDepartment of Anthropology, Temple University, Philadelphia, PA 19122; and dDepartment of Archaeology, School of Geography, Archaeology, and Earth Resources, University of Exeter, Exeter EX4 4QJ, United Kingdom Edited by Jeremy A. Sabloff, University of Pennsylvania Museum of Archaeology and Anthropology, Philadelphia, PA, and approved January 23, 2009 (received for review December 9, 2008) Questions that still surround the origin and early dispersals of maize lower montane (low mountain) forest occurs at 1,200 m, when oaks (Zea mays L.) result in large part from the absence of information on and other cooler-loving elements become a part of the flora (5). its early history from the Balsas River Valley of tropical southwestern We focused our analyses on starch grains and phytoliths, which Mexico, where its wild ancestor is native. We report starch grain and are effective indicators of wild and domesticated maize and phytolith data from the Xihuatoxtla shelter, located in the Central squash remains in archaeological contexts (6–14). As is common Balsas Valley, that indicate that maize was present by 8,700 calen- in other tropical regions (6, 7, 10–13), the preservation of drical years ago (cal. B.P.). Phytolith data also indicate an early macrofossil plant remains was very poor, consisting of wood preceramic presence of a domesticated species of squash, possibly charcoal and a few unidentified seed fragments.
    [Show full text]
  • Leaf Photosynthesis and Carbon Metabolism Adapt to Crop Load in ‘Gala’ Apple Trees
    horticulturae Article Leaf Photosynthesis and Carbon Metabolism Adapt to Crop Load in ‘Gala’ Apple Trees Xiaohua Yang 1, Li-Song Chen 1,2 and Lailiang Cheng 1,* 1 Section of Horticulture, School of Integrative Plant Science, Cornell University, Ithaca, NY 14850, USA; [email protected] (X.Y.); [email protected] (L.-S.C.) 2 College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China * Correspondence: [email protected] Abstract: It is widely accepted that a tight coordination between carbon (C) utilization in the sink and C assimilation and metabolism in the source exists in higher plants. However, much of our current understanding is based on research from herbaceous plants, where the source and sink interaction is less sophisticated compared to woody perennials with a significant sink presence. Apple (Malus x domestica Borkh.) is a good representative of the latter category, and its production and transport of sorbitol, in addition to sucrose, adds complexity to C regulation. In this study, four- year-old “Gala”/”M.26” apple trees were subjected to crop load levels at 2.5, 7.5, and 15 fruits/cm2 trunk cross-sectional area. Low crop load trees exhibited reduced leaf C assimilation and extra accumulation of non-structural carbohydrates (NSC). This was primarily a result of reduced activity of Rubisco and increased activities of key enzymes that synthesize starch, sucrose, and sorbitol. Among the NSC, leaf starch was found to be most sensitive to crop load and could function as a leading indicator for source–sink balance in apple. However, even the high crop load trees still retained a significant amount of NSC in leaves at dawn, demonstrating that apple is fundamentally Citation: Yang, X.; Chen, L.-S.; different from herbaceous plants in the way it balances leaf carbon inventories at dawn with carbon Cheng, L.
    [Show full text]
  • Trials and Tribulations of Ancient Starch Research: An
    TRIALS AND TRIBULATIONS OF ANCIENT STARCH RESEARCH: AN INVESTIGATION OF CONTAMINATION AND EARTH OVENS AT FORT HOOD, TEXAS A Dissertation by ANDREW ROBERT LAURENCE Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Approved by: Chair of Committee, Alston V. Thoms Co-Chair of Committee, Vaughn M. Bryant Committee Members, David L. Carlson Andreas Holzenburg Head of Department, Cynthia Werner May 2013 Major Subject: Anthropology Copyright 2013 Andrew Robert Laurence ABSTRACT Historically, earth ovens have been used to provide direct evidence of ancient plant use through the recovery of charred macrobotanical remains and indirectly by means of experimental archaeology and the ethnographic record. Experiments suggest that direct evidence of ancient starch-rich plant use can be obtained through the recovery of starch granules deposited on fire-cracked-rock (FCR) during cooking episodes even in regions where macrobotanical remains are scarcely preserved. Starch contamination, however, can enter into the archaeological record providing “background noise.” Therefore, this study analyzes the results of the Paluxy Sand Geophyte Project to determine if archaeological starch (starch that is both cultural and ancient in origin) can be differentiated from contamination using FCR recovered from heating elements in well-preserved earth ovens at Fort Hood, Texas. FCR, non-cultural rock control samples (RCS), and air control samples (ACS) were processed and analyzed from 27 earth ovens at 6 sites. Contamination control measures were used, including the use of a clean bench, powder-free latex gloves, washing samples prior to processing, spot sampling, and comparisons between st arch granule assemblages recovered from FCR and control samples.
    [Show full text]
  • Temporal and Spatial Patterns of Growth and Photosynthesis in Leaves of Dicotyledonous Plants
    Temporal and Spatial Patterns of Growth and Photosynthesis in Leaves of Dicotyledonous Plants Under Long-Term CO2- and O3-Exposure Inaugural Dissertation zur Erlangung des Doktorgrades der Mathematisch-Naturwissenschaftlichen Fakultät der Heinrich-Heine-Universität Düsseldorf vorgelegt von Diplom-Biologin Maja Mareike Christ aus Hannover 2005 Gedruckt mit der Genehmigung der Mathematisch-Naturwissenschaftlichen Fakultät der Heinrich-Heine-Universität Düsseldorf Referent: Prof. Dr. Ulrich Schurr Korreferent: PD. Dr. Dirk Gansert Tag der mündlichen Prüfung: 22.06.2005 para Andrés Abstract The aim of this Ph.D. thesis was to investigate spatio-temporal effects of elevated [CO2] and [O3] on leaf growth and photosynthesis in dicotyledonous plants. High-resolution spatio-temporal patterns of leaf growth were mea- sured with a digital image sequence processing method (DISP). For further analysis with the DISP method, the accuracy and resolution was estimated. The results led to the conclusion that temporal courses of leaf RGR could be analyzed with a resolution of at least 1-h-means, while 24-h-means could be used for heterogeneity determination, that requires a high spatial resolution. Effects of elevated [CO2] were investigated on Populus deltoides trees at Biosphere 2 Center, AZ, USA. Af- ter four years in elevated [CO2] (800 and 1200 ppm), Populus deltoides did not show acclimation. In 1200 ppm, net CO2 exchange rate was stimulated by a factor of 2–3 in growing leaves, accompanied by reduced stomatal conductance and transpiration rate, resulting in increased water use efficiency (WUE) by a factor of 2. Dark respiration was increased by a factor of ca. 1.5 in growing leaves.
    [Show full text]
  • University of Nevada, Reno Starch Residue Analysis from Two High
    University of Nevada, Reno Starch Residue Analysis from Two High Altitude Village Locations: High Rise Village, Wyoming and the White Mountain Village Sites, California A thesis submitted in partial fulfillment of the requirements for the degree of Master of Arts in Anthropology by Amanda M. Rankin Dr. Christopher T. Morgan/Thesis Advisor August, 2016 THE GRADUATE SCHOOL We recommend that the thesis prepared under our supervision by AMANDA M. RANKIN Entitled Starch Residue Analysis from Two High Altitude Village Locations: High Rise Village, Wyoming and the White Mountain Village Sites, California be accepted in partial fulfillment of the requirements for the degree of MASTER OF ARTS Dr. Christopher T. Morgan, Advisor Dr. Dave Rhode, Committee Member Dr Robert Watters, Graduate School Representative David W. Zeh, Ph.D., Dean, Graduate School August, 2016 i Abstract Starch residue analysis, ground stone, and use-wear analysis on milling equipment from High Rise Village and the White Mountain Village sites reveals a subsistence system that included geophyte processing at high elevation. High altitude residential use is little understood in North America and has often been thought to relate to intensive pine nut exploitation. This research indicates that this is not the case, and that geophytes were a targeted resource at high elevation. A closer look at the archaeological record in the two regions reveals that root processing was a common occurrence in nearby lowland regions and that high altitude villages may fit into this broader regional pattern of geophyte processing, a fact that has been overlooked by archaeologists and ethnographers alike, and something starch residue analysis is well suited to demonstrate.
    [Show full text]