TFPI2 Antibody (R30353)

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TFPI2 Antibody (R30353) Catalog No. Formulation Size R30353 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug Bulk quote request Availability 1-3 business days Species Reactivity Human Format Antigen affinity purified Clonality Polyclonal (rabbit origin) Isotype Rabbit IgG Purity Antigen affinity Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal UniProt P48307 Localization Cytoplasmic Applications Western blot : 0.5-1ug/ml IHC (FFPE) : 0.5-1ug/ml Limitations This TFPI2 antibody is available for research use only. Western blot testing of TFPI2 antibody and human lysates from Lane 1: MM453; 2: MM231; 3: HeLa; 4: HT1080; 5: Jurkat. Expected molecular weight: ~27 kDa (unmodified), 30-35 kDa (glycosylated). IHC-P: TFPI2 antibody testing of human breast cancer tissue Description Tissue factor pathway inhibitor 2, also known as TFPI2, is a human gene which is located at 7q22. It is an important regulator of the extrinsic pathway of blood coagulation through its ability to inhibit factor Xa and factor VIIa-tissue factor activity. After a 22-residue signal peptide, the mature TFPI2 protein contains 213 amino acids with 18 cysteines and 2 canonical N-linked glycosylation sites. The purified recombinant TFPI2 strongly inhibited the amidolytic activities of trypsin and the factor VIIa-tissue factor complex. The latter inhibition was markedly enhanced in the presence of heparin. Mouse TFPI2 mRNA is highly expressed in developing mouse placenta, as in human. And there are also high transcript levels in adult mouse liver and kidney. Application Notes The stated application concentrations are suggested starting amounts. Titration of the TFPI2 antibody may be required due to differences in protocols and secondary/substrate sensitivity. Immunogen An amino acid sequence from the middle region of human TFPI2 (NVTRYYFNPRYRTCDAF) was used as the immunogen for this TFPI2 antibody. Storage After reconstitution, the TFPI2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing. Ordering: Phone:858.663.9055 | Fax:1.267.821.0800 | Email:[email protected] Copyright © NSJ Bioreagents. All rights reserved.

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MEG8 Regulates Tissue Factor Pathway Inhibitor 2 (TFPI2) Expression in the Endothelium
MEG8 regulates Tissue Factor Pathway Inhibitor 2 (TFPI2) expression in the endothelium Veerle Kremer VU Medical Center, Amsterdam UMC Diewertje I. Bink VU Medical Center, Amsterdam UMC Laura Stanicek VU Medical Center, Amsterdam UMC Eva van Ingen Leiden University Medical Center Sarah Hilderink VU Medical Center, Amsterdam UMC Anne Yaël Nossent Leiden University Medical Center Reinier A. Boon ( [email protected] ) VU Medical Center, Amsterdam UMC Research Article Keywords: human umbilical vein endothelial cells (HUVECs), Tissue Factor Pathway Inhibitor 2 (TFPI2) Posted Date: August 13th, 2021 DOI: https://doi.org/10.21203/rs.3.rs-800527/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/19 Abstract A large portion of the genome is transcribed into non-coding RNA, which does not encode protein. Many long non-coding RNAs (lncRNAs) have been shown to be involved in important regulatory processes such as genomic imprinting and chromatin modication. The 14q32 locus contains many non-coding RNAs such as Maternally Expressed Gene 8 (MEG8). We observed an induction of this gene in ischemic heart disease. We investigated the role of MEG8 specically in endothelial function as well as the underlying mechanism. We hypothesized that MEG8 plays an important role in cardiovascular disease via epigenetic regulation of gene expression. Experiments were performed in human umbilical vein endothelial cells (HUVECs). In vitro silencing of MEG8 resulted in impaired angiogenic sprouting. More specically, total sprout length was reduced as was proliferation, while migration was unaffected. We performed RNA sequencing to assess changes in gene expression after loss of MEG8.
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