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30Th RAU Abstract Book 1 2 Dear colleagues, On behalf of the Organizing Committee, I am pleased to welcome all the participants to the 30th edition of the Annual Users Meeting of the Brazilian Synchrotron Light Laboratory- RAU/LNLS. I would like to start by wishing you and your families my personal best for your health and safety in these difficult times. This year has tested us like no other. RAU is a unique opportunity to gather together the Brazilian lightsource community in a single scientific event to disseminate results and exchange ideas. Following much debate, we decided to proceed with the RAU in the now realized alternative format. This was not without its own challenges. The year 2020 marks the 30th anniversary of this Users Meeting. Although RAU is being run under exceptional circumstances, we have created what we hope will be a RAU to be remembered for many years to come. 30th RAU will be four days long bringing together around 500 researchers from different corners of Brazil and from more than 20 countries around the world. The programme includes 114 poster presentations, 20 oral contributions, 4 keynote talks and 6 invited plenary lectures. In addition, we will have an overview of LNLS, Sirius virtual tour, meetings with beamline coordinators, and last but not the least, the newly elected User Committee will be presented during the LNLS Users Group assembly. The structure of this RAU was designed to stimulate discussion and participation. All sessions will have life in them. We certainly encourage all participants to attend and engage with sessions as though you were here in person. We are privileged to hear the highlights from this year’s hard work on Sirius beamlines and to take part in a rich, and highly relevant, discussion on cutting-edge research to stimulate new proposals and ideas. We believe many good experiences will be shared and good lessons learned. We also want to express our optimism about the future, with the Sirius moving along, ready to go for another 30 years. Sirius currently works with a stable circulating current in the accelerators at around 10% of the current expected for its full operation. In the last few months, the first experiments on the MANACÁ beamline were carried out by external researchers dedicated to studying the molecular mechanisms related to the action of the SARS-CoV-2 virus. The other beamlines of the first phase (CARNAÚBA, CATERETÊ, EMA, IPÊ, MOGNO) are in an advanced stage of assembly. We cannot fail to register our sincere gratitude and our recognition to professor Ricardo Rodrigues. Finally, we would like to thank this incredible Local Committee and the CNPEM staff for organizing this RAU, ensuring it ran so smoothly. We would also like to take this opportunity to thank our sponsors who supported the move to an online meeting. Take care of yourselves and stay healthy. Hope to see you all soon! Regina Cély Barroso Chair of the 30th RAU 3 4 INVITED ORAL PRESENTATIONS 3D IMAGING OF BIOLOGICAL TISSUES BY X-RAY PTYCHOGRAPHIC TOMOGRAPHY .................... 12 ARE YOU MEASURING WHAT YOU THINK YOU’RE MEASURING? RADIATION INDUCED DAMAGE IN X-RAY FLUORESCENCE MICROSCOPY ............................................................................................ 13 BACTERIAL SENSORY MACHINES, WATCH THEM AS THEY MOVE ................................................... 14 BREAKTHROUGHS IN MATERIAL CHARACTERIZATION AT EXTREME CONDITIONS WITH SYNCHROTRON AND OPTICAL TECHNIQUES ....................................................................................... 15 HETEROGENEOUS CHEMISTRY AT AQUEOUS INTERFACES INVESTIGATED WITH AMBIENT PRESSURE X-RAY PHOTOELECTRON SPECTROSCOPY .................................................................... 16 HIGH RESOLUTION STRUCTURES OF THE SARS-CoV-2 NSP16/NSP10 2’-O- METHYLTRANSFERASE REVEALS STRATEGIES FOR STRUCTURE-BASED INHIBITOR DESIGN . 17 ORAL PRESENTATIONS ACTIVITY OF N2O REDUCTION BY CO AND THE EVOLUTION OF COPPER SPECIES ....................... 18 APPLICATION OF X-RAY COMPUTED MICRO-TOMOGRAPHY TECHNIQUE IN UNDERSTANDING THE DYNAMICS OF FLUID FLOW IN POROUS ....................................................................................... 19 ASSESSING THE ROLE OF ELEMENTS IN CANCER PROGRESSION WITH SR-XRF AND DATA MINING ....................................................................................................................................................... 20 BIOCHEMICAL AND STRUCTURAL CHARACTERIZATION OF LSFA, A 1-CYS PEROXIREDOXIN INVOLVED WITH PSEUDOMONAS AERUGINOSA VIRULENCE ............................................................ 21 DEPICTING X-RAY RADIATION DAMAGE TO IN VIVO PLANT TISSUES: A GLANCE ON ELEMENTAL, ULTRASTRUCTURAL, AND HISTOCHEMICAL RESPONSES ................................................................. 22 DYNAMICS OF FERROELASTIC DOMAINS IN CsPbBr3 NANOWIRES IMAGED BY IN SITU NANOFOCUSED SCANNING X-RAY DIFFRACTION ............................................................................... 23 FERROMAGNETISM IN TiO2 AND ZnO OXIDES BY HYDROGEN INCORPORATION ........................... 24 FOLLOWING PROTEIN CORONA FORMATION ONTO NANOPARTICLES BY MEANS OF SAXS AND COMPLEMENTARY TECHNIQUES ........................................................................................................... 25 GIWAXS STUDY ON THE ANNEALING TIMES OPTIMIZATION OF CONJUGATED POLYMERS FOR OPV APPLICATION .................................................................................................................................... 26 HOMOGENEOUS SYNTHESIS OF GOLD NANOPARTICLES: KINETICS, MECHANISMS, AND RELATED ONE-POT COMPOSITES ......................................................................................................... 27 IMAGING AND ANALYZING NEURAL TUBE DURING MOUSE EMBRYONIC DEVELOPMENT: NEW TOOLS WITH SYNCHROTRON RADIATION TO STUDY THIS COMPLEX 3D STRUCTURE ................. 28 IN SITU XAS: A POWERFUL INVESTIGATION TOOL FOR PALLADIUM-MEDIATED BIOORTHOGONAL UNCAGING REACTIONS IN CANCER CELLS .......................................................................................... 29 INVESTIGATION OF EXOGENEOUS PULMONARY SURFACTANTS BY WIDE- AND SMALL-ANGLE X-RAY SCATTERING ................................................................................................................................. 30 5 LOCALIZATION AND SPECIATION OF CARBON OCCLUDED IN SILICA PHYTOLITHS FROM GRASSES AS AN INITIAL STEP TO QUANTIFY ITS GLOBAL POTENTIAL FOR SOIL CARBON SEQUESTRATION STRATEGIES .............................................................................................................. 31 POLARIZATION-DEPENDENT ARPES ORBITAL STUDIES OF CO AND Cu DOPED BaFe2As2 BAND STRUCTURE .............................................................................................................................................. 32 PRESSURE-INDUCED STRUCTURAL PHASE TRANSITION AND ANOMALOUS PHOTOLUMINESCENCE BEHAVIOR IN LUMINESCENT BULK Cs4PbBr6 SINGLE CRYSTALS ............ 33 STRUCTURAL-DEPENDENT PHOTOLUMINESCENT BEHAVIOR OF CsPb2(Br0.85/I0.15)5 ...................... 34 STUDY OF INDUCED SPIN POLARIZATION OF Cu ATOMS IN Fe/Co/Cu3Au(001): AN EXPERIMENTAL AND FIRST-PRINCIPLES INVESTIGATION .............................................................................................. 35 SURFACE COMPOSITION OF AEROSOL MODEL SOLUTIONS CONTAINING INORGANIC SALT AND AMINO ACID............................................................................................................................................... 36 SYNCHROTRON RADIATION-BASED X-RAYS FLUORESCENCE AND ABSORPTION SPECTROSCOPIES TO BOOST STUDIES OF AGRONOMIC BIOFORTIFICATION AND METAL SPECIATION IN TROPICAL SOIL ENVIRONMENT .................................................................................. 37 THE ACTIVE WOX SITES OF Mn-Na2WO4/SiO2 CATALYST FOR THE OXIDATIVE COUPLING OF METHANE .................................................................................................................................................. 38 THE INFLUENCE OF CeO2-x (0 < X < 0.5) PROPERTIES ON THE REACTIVITY OF Cu-CeO2-X NANOPARTICLES TOWARDS THE CO OXIDATION REACTION ............................................................ 39 VALENCE ELECTRON EXCITATIONS IN LiH INVESTIGATED BY IXS SPECTROSCOPY .................... 40 X-RAY PROTEIN CRYSTALLOGRAPHY REVEALS UNIQUE FUNCTIONS AND COMPONENTS IN A PHOSPHO-SIGNALING SYSTEM THAT CONTROLS METABOLISM AND VIRULENCE OF MYCOBACTERIUM TUBERCULOSIS ....................................................................................................... 41 POSTER PRESENTATIONS 3D-PRINTED MICROFLUIDIC SAMPLE HOLDER DEVICE FOR SERIAL SYNCHROTRON X-RAY CRYSTALLOGRAPHY ............................................................................................................................... 42 A COMPACT SPECTROMETER BASED ON A SEGMENTED CONICAL CRYSTAL ANALYZER ........... 43 A MACHINE LEARNING APPROACH APPLIED TO DETERMINE FORMAL OXIDATION STATE OF 3D COMPOUNDS ............................................................................................................................................ 44 A NEW QUARTER WAVE PLATE INSTRUMENTATION FOR FAST SWITCHING THE X-RAY HELICITY .................................................................................................................................................................... 45 ACCELERATING LIGHT IN TWO-DIMENSIONAL CRYSTALS ................................................................ 46 ADSORPTION OF METHYLENE BLUE DYE IN AQUEOUS SOLUTIONS USING
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