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WO 2019/014655 Al 17 January 2019 (17.01.2019) W !P O PCT

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WO 2019/014655 Al 17 January 2019 (17.01.2019) W !P O PCT (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2019/014655 Al 17 January 2019 (17.01.2019) W !P O PCT (51) International Patent Classification: A61Q 5/02 (2006.01) A61Q 19/10 (2006.01) (21) International Application Number: PCT/US20 18/042 192 (22) International Filing Date: 14 July 2018 (14.07.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/532,364 14 July 2017 (14.07.2017) US (71) Applicant: CLEANSPOT, INC. [US/US]; 5901 Indian School Road NE, Albuquerque, NM 871 10 (US). (72) Inventors: CORBITT, Thomas, S.; 5901 Indian School Road NE, Albuquerque, NM 871 10 (US). CALL, Charles, J.; 5901 Indian School Road NE, Albuquerque, NM 871 10 (US). CHAVEZ, Ana, M.; 5901 Indian School Road NE, Albuquerque, NM 871 10 (US). (74) Agent: CHELLAPPA, Anand, S.; Ip Tekk, LLC, 8100 Wyoming Blvd NE, Suite M4, #339, Albuquerque, NM 871 13 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) (54) Title: SANITIZING COMPOSITIONS AND METHODS FOR MAKING AND USING SAME © (57) Abstract: Sanitizing compositions and methods for making and using these compositions are disclosed. In particular, the compo sitions comprise a fast acting antimicrobial agent and a persistent microbial agent with alcohol and beeswax among the active ingredi- ents. The ethanol content may vary between about 62 vol.-% and 75 vol.-%. The beeswax content may vary between about 0.01 vol.- % and 3 vol.-%. Log reduction in E. coli bacteria > 5 was measured at 1 minute, 8 hours and 24 hours. SANITIZING COMPOSITIONS AND METHODS FOR MAKING AND USING SAME RELATED APPLICATIONS [0001] This application is related to and claims the benefit of U.S. Provisional Patent Application 62/532,364, filed July 14, 2017, and entitled "Sanitizing Compositions and Methods for Making and Using Same," which is hereby incorporated by reference in its entirety. FEDERALLY SPONSORED RESEARCH AND DEVELOPMENT [0002] Not applicable. FIELD [0003] This disclosure relates to sanitizing compositions and methods for making and using these compositions. In particular, the compositions comprise a fast acting antimicrobial agent and a persistent microbial agent with alcohol and beeswax among the active ingredients. BACKGROUND [0004] Sanitizing compositions may be used for sanitizing a variety of surfaces other than human and animal skin. U.S. Patent Application No. 2008/0045491 entitled "SURFACE SANITIZER" discloses compositions formulated as solutions for sanitizing hard surfaces such as countertops and floors, or as solutions/gels for application to animal skin. The non-toxic antimicrobial surface sanitizer composition comprises a water- miscible alcohol (50 wt.-% to 90 wt.-%), water (10 wt.-% to 50 wt.-%), an acid component (0. 1 wt.-% to 10 wt.-%) sufficient to maintain the pH of the composition below about 5, and a multivalent cation (e.g., metal ion or metal compound) comprising 0.01 wt.-% to 5 wt.-%. The acid component comprises one or more weak acids having pKa values between about 2 and about 5, and provide persistent antiviral protection after the volatile components have evaporated. The water-miscible alcohol component is at least one of ethanol, n-propanol, isopropanol, n-butanol, 2-butanol, iso butanol and tert- butanol. The water-miscible alcohol component has a dielectric constant that is less than about 23. The acid component is at least one of phosphoric acid, acetic acid, propionic acid, citric acid, glycine, malic acid, glycolic acid, salicylic acid, benzoic acid, ascorbic acid and succinic acid. The composition may also include one or more of an emollient comprising 0.01 wt.-% to 0.5 wt.-%, oxidative agent, humectant comprising 0.01 and 1%, lubricants such as beeswax, jojoba oil, lanolin, almond oil, olive oil, shea butter comprising 0.01 wt.-% to 0.5 wt.-%, plant-derived alkene, and plant-derived essential oil. The multivalent cation component includes polymers having at least two positive charges such as polyamines (e.g., polyvinylamine, polyethylenimine, putrescine, spermidine, spermine), chitosan, polylysine, metal ions and metal compounds. The metal component is at least one of aluminum, bismuth, silver, zinc, boron, selenium and nickel. The metal may be provided in the form of a water-soluble salt, organic compound, inorganic compound, dispersion of finely-divided metal, insoluble salt or compound and are used to increase the viscosity of the solution and/or forming a gel, which is beneficial in the preparation a hand sanitizer comprising low pH solutions. Composition that are acidic (pH < 5) may provide for persistent antimicrobial activity but are not desirable for sanitizing skin and for sanitizing most surfaces. [0005] Alcohols are considered to be "fast-acting" sanitizing agents. Hand sanitizers with at least a 62 vol.-% ethanol are effective in killing bacteria, such as the Streptococcus bacteria, and are also effective against fungal infections, and common cold and flu viruses. Ethanol and isopropanol kill these microbes/germs by denaturing the protein content and dissolving or lysing their essential proteins. At alcohol content of 62 wt.-% or more, the germs are destroyed in 10 to 15 seconds. Isopropyl alcohol may destroy >99.99% (4 log reduction) of most bacteria in < 30 seconds. It has been reported that alcohol rub sanitizers containing at least 62 wt.-% alcohol kill 99.9% (3 log reduction) of the bacteria in about 30 seconds after application, and > 99.99% in about 60 seconds. Upon re-exposure to these germs, another application of alcohol containing sanitizing agents would be required to destroy these germs. In hospitals and clinics, the alcohol content in sanitizing compositions used to treat contaminated surfaces is typically 60-95% . These formulations are effective against viruses as well. Sanitizing compositions with high ethanol content are flammable. In addition, it has been reported that children are tempted to ingest high-ethanol containing compositions. Alcohol-based hand sanitizers may cause alcohol poisoning in small children. Further, sanitizing compositions that contain only alcohols as the active ingredients do not have long-lasting or "persistent" sanitizing properties, due to the volatility of the alcohols. [0006] Sanitizers such as PURELL® typically comprise ethyl alcohol as the active ingredient, and other agents such as isopropyl alcohol, carbomer to form a non-foaming gel, tocopheryl acetate (fat soluble vitamin E) as an antioxidant and moisturizer (humectant) to minimize the drying effects of ethanol, glycerol as a moisturizer, propylene glycol as a moisturizer, and isopropyl myristate to balance the consistency of the gel and emollient. [0007] Some hand sanitizer products may be alcohol free and contain sanitizing agents such as povidone-iodine, benzalkonium chloride, and triclosan to kill microorganisms. Although low amounts (0.1 wt.-% to 0.2 wt.-%) of these agents may have the same antimicrobial effects as compositions that contain at least 62 vol.-% ethanol, formulations with triclosan as the active agent have shown high toxicity against infant dermal fibroblasts. Povidone-iodine solutions have been proven to result in dermatitis in as little as 2% iodine in solution, and have been shown to inhibit human fibroblast growth. In contrast to alcohols, some microorganisms may develop a resistance to these agents. U.S. Patent No. 7,1 12,559 entitled "THICKENED QUATERNARY AMMONIUM COMPOUND SANITIZER" discloses hand sanitizing compositions that have less than about 30 wt.-% of the total composition of alcohols. The compositions consist of a quaternary ammonium compound (0.01 wt.-% to 3 wt.-%) that provides antimicrobial activity, a thickener in an amount effective (0.001 wt.-% to 5 wt.-%) to provide a viscosity from about 200 to about 5,000 centipoise, and a solvent such as water, ethanol and isopropanol (25 wt.-% to 99 wt.-%). Additives such as pH adjuster, skin conditioner, drying time enhancer, dye, fragrance and mixtures thereof may also be used. The disclosed quaternary ammonium compounds are monoalkyldimethylbenzyl ammonium salts, dialkyldimethyl ammonium salts, polysubstituted quaternary ammonium salts, bis- quaternary ammonium salts, benzalkonium chloride, benzethonium chloride and mixtures. The quaternary ammonium compound, the thickener, and the solvent are considered to be food additives. [0008] "Persistent" sanitizing activity generally refers to extended antimicrobial activity that inhibits the proliferation of microorganisms after application of the sanitizing composition over some extended period of time. As opposed to "fast-acting" ingredients, the time scale for sanitizing could be in the order of hours. For example, NimbuDerm™ (Quick-Med Technologies Inc.) is reported to be an alcohol-free, copolymer based persistent hand sanitizer that provides for long lasting antimicrobial protection, for up to 8 hours.
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