Setting a Protocol for Identification and Detecting the Prevalence of Candida Auris in Tertiary Egyptian Hospitals Using the CDC Steps
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Candida Krusei: Biology, Epidemiology, Pathogenicity and Clinical Manifestations of an Emerging Pathogen
J. Med. Microbiol. - Vol. 41 (1994), 295-310 0 1994 The Pathological Society of Great Britain and Ireland REVIEW ARTICLE: CLINICAL MYCOLOGY Candida krusei: biology, epidemiology, pathogenicity and clinical manifestations of an emerging pathogen YUTHIKA H. SAMARANAYAKE and L. P. SAMARANAYAKE” Department of Pathology (Oral), Faculty of Medicine and Ord diology Unit, Faculty of Dentistry, University of Hong Kong, 34 Hospital Road, Hong Kong Summary. Early reports of Candida krusei in man describe the organism as a transient, infrequent isolate of minor clinical significance inhabiting the mucosal surfaces. More recently it has emerged as a notable pathogen with a spectrum of clinical manifestations such as fungaemia, endophthalmitis, arthritis and endocarditis, most of which usually occur in compromised patient groups in a nosocomial setting. The advent of human immunodeficiency virus infection and the widespread use of the newer triazole fluconazole to suppress fungal infections in these patients have contributed to a significant increase in C. krusei infection, particularly because of the high incidence of resistance of the yeast to this drug. Experimental studies have generally shown C. krusei to be less virulent than C. albicans in terms of its adherence to both epithelial and prosthetic surfaces, proteolytic potential and production of phospholipases. Furthermore, it would seem that C. krusei is significantlydifferent from other medically important Candida spp. in its structural and metabolic features, and exhibits different behaviour patterns towards host defences, adding credence to the belief that it should be re-assigned taxonomically. An increased awareness of the pathogenic potential of this yeast coupled with the newer molecular biological approaches to its study may facilitate the continued exploration of the epidemiology and pathogenesis of C. -
Candida Auris
microorganisms Review Candida auris: Epidemiology, Diagnosis, Pathogenesis, Antifungal Susceptibility, and Infection Control Measures to Combat the Spread of Infections in Healthcare Facilities Suhail Ahmad * and Wadha Alfouzan Department of Microbiology, Faculty of Medicine, Kuwait University, P.O. Box 24923, Safat 13110, Kuwait; [email protected] * Correspondence: [email protected]; Tel.: +965-2463-6503 Abstract: Candida auris, a recently recognized, often multidrug-resistant yeast, has become a sig- nificant fungal pathogen due to its ability to cause invasive infections and outbreaks in healthcare facilities which have been difficult to control and treat. The extraordinary abilities of C. auris to easily contaminate the environment around colonized patients and persist for long periods have recently re- sulted in major outbreaks in many countries. C. auris resists elimination by robust cleaning and other decontamination procedures, likely due to the formation of ‘dry’ biofilms. Susceptible hospitalized patients, particularly those with multiple comorbidities in intensive care settings, acquire C. auris rather easily from close contact with C. auris-infected patients, their environment, or the equipment used on colonized patients, often with fatal consequences. This review highlights the lessons learned from recent studies on the epidemiology, diagnosis, pathogenesis, susceptibility, and molecular basis of resistance to antifungal drugs and infection control measures to combat the spread of C. auris Citation: Ahmad, S.; Alfouzan, W. Candida auris: Epidemiology, infections in healthcare facilities. Particular emphasis is given to interventions aiming to prevent new Diagnosis, Pathogenesis, Antifungal infections in healthcare facilities, including the screening of susceptible patients for colonization; the Susceptibility, and Infection Control cleaning and decontamination of the environment, equipment, and colonized patients; and successful Measures to Combat the Spread of approaches to identify and treat infected patients, particularly during outbreaks. -
1. Economic, Ecological and Cultural Importance of Fungi
1. Economic, ecological and cultural importance of Fungi Fungi as food Yeast fermentations, Saccaromyces cerevesiae [Ascomycota] alcoholic beverages, yeast leavened bread Glucose 2 glyceraldehyde-3-phosphate + 2 ATP 2 NAD O2 2 NADH2 2 pyruvate + 2 ATP + 2 H2O 2 ethanol 2 acetaldehyde + 2 ATP + 2 CO2 Fungi as food Citric acid Aspergillus niger Fungi as food Cheese Penicillium camembertii, Penicillium roquefortii Rennet, chymosin produced by Rhizomucor miehei and recombinant Aspergillus niger, Saccharomyces cerevesiae chymosin first GM enzyme approved for use in food Fungi as food Quorn mycoprotein, produced from biomass of Fusarium venenatum [Ascomycota] Fungi as food Red yeast rice, Monascus purpureus Soy fermentations, Aspergillus oryzae [Ascomycota] contains lovastatin? Tempeh, made with Rhizopus oligosporus [Zygomycota] Fungi as food Other fungal food products: vitamins and enzymes • vitamins: riboflavin (vitamin B2), commercially produced by Ashbya gossypii • chocolate: cacao beans fermented before being made into chocolate with a mixture of yeasts and filamentous fungi: Candida krusei, Geotrichum candidum, Hansenula anomala, Pichia fermentans • candy: invertase, commercially produced by Aspergillus niger, various yeasts, enzyme splits disaccharide sucrose into glucose and fructose, used to make candy with soft centers • glucoamylase: Aspergillus niger, used in baking to increase fermentable sugar, also a cause of “baker’s asthma” • pectinases, proteases, glucanases for clarifying juices, beverages Fungi as food Perigord truffle, Tuber -
Council of State and Territorial Epidemiologists
Appendix 1 Identification of Candida auris (as of August 20, 2018). This appendix will be updated as new information about C. auris identification becomes available. Some yeast identification methods are unable to differentiate C. auris from other yeast species. C. auris can be misidentified as a number of different organisms when using traditional biochemical methods for yeast identification such as VITEK 2 YST, API 20C, BD Phoenix yeast identification system, and MicroScan. The most common misidentification of C. auris is Candida haemulonii. C. haemulonii have been less commonly observed to cause invasive infections. Therefore, C. auris should be suspected when C. haemulonii is identified on culture of blood or other normally sterile site unless the method used can reliably detect C. auris. Candida isolates from the urine and respiratory tract ultimately confirmed as C. auris have been initially identified as C. haemulonii; less data are available about the ability of C. haemulonii to grow in urine or the respiratory tract, although true C. haemulonii infections in general appear to be rare in the United States. The table below summarizes common misidentifications based on the yeast identification method used. If any of the species listed below are identified using the specified identification method, or if species identity cannot be determined by any method, further characterization using appropriate methodology should be sought. Common misidentifications for C. auris by yeast identification method Identification Method Organism C. auris can be misidentified as No misidentifications of Candida auris. Bruker Bruker MALDI Biotyper (FDA database) MALDI-TOF is able to accurately identify C. auris bioMérieux VITEK MS (IVD/RUO database) Candida haemulonii Candida haemulonii VITEK 2 YST (Ver. -
Candida Krusei
P.O. Box 131375, Bryanston, 2074 Ground Floor, Block 5 Bryanston Gate, Main Road Bryanston, Johannesburg, South Africa www.thistle.co.za Tel: +27 (011) 463 3260 Fax: +27 (011) 463 3036 e-mail : [email protected] Please read this bit first The HPCSA and the Med Tech Society have confirmed that this clinical case study, plus your routine review of your EQA reports from Thistle QA, should be documented as a “Journal Club” activity. This means that you must record those attending for CEU purposes. Thistle will not issue a certificate to cover these activities, nor send out “correct” answers to the CEU questions at the end of this case study. The Thistle QA CEU No is: MT00025. Each attendee should claim THREE CEU points for completing this Quality Control Journal Club exercise, and retain a copy of the relevant Thistle QA Participation Certificate as proof of registration on a Thistle QA EQA. Cycle 22 Organism 6: Candida non-albicans species - Candida krusei Candida species are ubiquitous yeasts, found on many plants and as members of the normal flora of the alimentary tract of mammals and mucocutaneous membrane of humans. All areas of the human gastrointestinal tract can harbor Candid. The most commonly isolated species (50% to 70% of yeast isolates) from human gastrointestinal tract is Candida albicans, followed by Candida tropicalis, Candida parapsilosis, and Candida glabrata. C. glabrata is regarded as a symbiont of humans and can be isolated routinely from the oral cavity and from the gastrointestinal tract, genitourinary, and respiratory tracts of most individuals. As an agent of serious infection it has been associated with endocarditis, meningitis, and multifocal disseminated disease. -
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Current Medical Mycology 2019, 5(4): 26-34 Species distribution and susceptibility profiles of Candida species isolated from vulvovaginal candidiasis, emergence of C. lusitaniae Seyed Ebrahim Hashemi1, Tahereh Shokohi2, 3*, Mahdi Abastabar2, 3, Narges Aslani4, Mahbobeh Ghadamzadeh5, Iman Haghani3 1 Student Research Committee, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran 2 Invasive Fungi Research Centre (IFRC), Mazandaran University of Medical Sciences, Sari, Iran 3 Department of Medical Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran 4 Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran 5 Gynecology and Obstetrics Department of Hazrat-e- Zainab Hospital, Babolsar, Iran Article Info A B S T R A C T Article type: Background and Purpose: The aim of the current study was to investigate the Original article epidemiology of vulvovaginal candidiasis (VVC) and recurrent VVC (RVVC), as well as the antifungal susceptibility patterns of Candida species isolates. Materials and Methods: A cross-sectional study was carried out on 260 women suspected of VVC from February 2017 to January 2018. In order to identify Candida Article History: species isolated from the genital tracts, the isolates were subjected to polymerase chain Received: 02 August 2019 reaction restriction fragment length polymorphism (PCR-RFLP) using enzymes Msp I Revised: 20 October 2019 and sequencing. Moreover, antifungal susceptibility testing was performed according to Accepted: 10 November 2019 the Clinical and Laboratory Standards Institute guidelines (M27-A3). Results: Out of 250 subjects, 75 (28.8%) patients were affected by VVC, out of whom 15 (20%) cases had RVVC. Among the Candida species, C. -
Screening for Triazole Resistance in Clinically Signifcant Aspergillus Species; Report from Pakistan
Screening for triazole resistance in clinically signicant Aspergillus species; report from Pakistan Saa Moin Aga Khan University Joveria Farooqi ( [email protected] ) Aga Khan University https://orcid.org/0000-0002-9921-4660 Kauser Jabeen Aga Khan University Sidra Laiq Aga Khan University Aa Zafar Aga Khan University Research Keywords: Aspergillosis, Aspergillus avus, Aspergillus fumigatus, Aspergillus niger, Aspergillus terreus, itraconazole, voriconazole and posaconazole. Posted Date: April 3rd, 2020 DOI: https://doi.org/10.21203/rs.2.17755/v2 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Version of Record: A version of this preprint was published at Antimicrobial Resistance and Infection Control on May 11th, 2020. See the published version at https://doi.org/10.1186/s13756-020-00731-8. Page 1/17 Abstract Abstract Background: Burden of aspergillosis is reported to be signicant from developing countries including those in South Asia. The estimated burden in Pakistan is also high on the background of tuberculosis and chronic lung diseases. There is concern for management of aspergillosis with the emergence of azole resistant Aspergillus species in neighbouring countries in Central and South Asia. Hence the aim of this study was to screen signicant Aspergillus species isolates at the Microbiology Section of Aga Khan Clinical Laboratories, Pakistan, for triazole resistance. Methods: A descriptive cross-sectional study, conducted at the Aga Khan University Laboratories, Karachi, from September 2016- May 2019. One hundred and fourteen, clinically signicant Aspergillus isolates [ A. fumigatus (38; 33.3%), A. avus (64; 56.1%), A. niger (9; 7.9%) A. -
C. Auris Reporting Guidance
To: Healthcare Providers and Laboratorians From: Iowa Department of Public Health, Center for Acute Disease Epidemiology Re: Candida auris infection or Colonization in Iowa Residents as temporarily Reportable Date: December 4, 2020 Background: Candida auris is an emerging fungus that presents a serious global health threat. The CDC recommends that all Candida isolates obtained from normal sterile sites (e.g., bloodstream, cerebrospinal fluid) be identified to the species level as initial treatment can be administered based on the typical, species-specific susceptibility patterns. C. auris in a non-sterile body site is also very important to identify because the identification can represent wider colonization, which poses a risk for transmission and infection control precautions. If a laboratory identifies any Candida species from any site, submission of the isolate is warranted. Surveillance: All laboratories are to submit isolates from any site that include the following species: 1. Candida auris 2. Candida famata 3. Candida haemulonii 4. Candida sake 5. Rhodotorula glutinis 6. Saccharomyces cerevisiae 7. Candida spp. (isolates where the species is attempted and results are inconclusive). Algorithm to identify C. auris: The Centers for Disease Control and Prevention (CDC) provides an algorithm to identify C. auris based on phenotypic laboratory and initial species identification. The algorithm can be accessed at the following hyperlink: https://www.cdc.gov/fungal/diseases/candidiasis/pdf/Testing-algorithm-by-Method-temp.pdf. Specimen Submission: C. auris can be misidentified as a number of different organisms when using traditional phenotypic methods for yeast identification such as VITEK 2 YST, API 20C, BD Phoenix yeast identification system, and MicroScan. -
Hospital Laboratory Survey for Identification of Candida Auris In
Journal of Fungi Article Hospital Laboratory Survey for Identification of Candida auris in Belgium Klaas Dewaele 1 , Katrien Lagrou 2,3, Johan Frans 1, Marie-Pierre Hayette 4 and Kris Vernelen 5,* 1 Department of Clinical Microbiology, Imelda General Hospital, 2820 Bonheiden, Belgium 2 Department of Laboratory Medicine and National Reference Centre for Mycosis, University Hospitals of Leuven, 3000 Leuven, Belgium 3 Department of Microbiology, Immunology and Transplantation, KU Leuven, 3000 Leuven, Belgium 4 Department of Clinical Microbiology and National Reference Center for Mycosis, University Hospital of Liège, 4000 Liège, Belgium 5 Quality of Laboratories, Sciensano, 1050 Brussels, Belgium * Correspondence: [email protected] Received: 4 August 2019; Accepted: 27 August 2019; Published: 5 September 2019 Abstract: Candida auris is a difficult-to-identify, emerging yeast and a cause of sustained nosocomial outbreaks. Presently, not much data exist on laboratory preparedness in Europe. To assess the ability of laboratories in Belgium and Luxembourg to detect this species, a blinded C. auris strain was included in the regular proficiency testing rounds organized by the Belgian public health institute, Sciensano. Laboratories were asked to identify and report the isolate as they would in routine clinical practice, as if grown from a blood culture. Of 142 respondents, 82 (57.7%) obtained a correct identification of C. auris. Of 142 respondents, 27 (19.0%) identified the strain as Candida haemulonii. The remaining labs that did not obtain a correct identification (33/142, 23.2%), reported other yeast species (4/33) or failed to obtain a species identification (29/33). To assess awareness about the infection-control implications of the identification, participants were requested to indicate whether referral of this isolate to a reference laboratory was desirable in a clinical context. -
Vaginal Colonization and Vulvovaginitis by Candida Species in Pregnant Women from Northern of Colombia
Archivos de Medicina (Col) ISSN: 1657-320X [email protected] Universidad de Manizales Colombia Vaginal colonization and vulvovaginitis by Candida species in pregnant women from Northern of Colombia Suárez, Paola; Belloz, Ana; Puelloz, Martha; Youngz, Gregorio; Duranz, Marlene; Arechavala, Alicia Vaginal colonization and vulvovaginitis by Candida species in pregnant women from Northern of Colombia Archivos de Medicina (Col), vol. 18, no. 1, 2018 Universidad de Manizales, Colombia Available in: https://www.redalyc.org/articulo.oa?id=273856494005 DOI: https://doi.org/10.30554/archmed.18.1.2010.2018 PDF generated from XML JATS4R by Redalyc Project academic non-profit, developed under the open access initiative Artículos de Investigación Vaginal colonization and vulvovaginitis by Candida species in pregnant women from Northern of Colombia Vulvovaginitis y colonización vaginal por especies de Candida en gestantes del norte de Colombia Resumen Paola Suárez [email protected] Cartagena University, Colombia Ana Belloz [email protected] Cartagena University., Colombia Martha Puelloz [email protected] Cartagena University., Colombia Gregorio Youngz [email protected] Cartagena University., Colombia Marlene Duranz [email protected] Cartagena University., Colombia Archivos de Medicina (Col), vol. 18, no. 1, 2018 Alicia Arechavala [email protected] Universidad de Manizales, Colombia Hospital de Infecciosas Muñiz, Buenos Aires, Argentina., Argentina Received: 10 June 2018 Corrected: 12 March 2018 Accepted: 12 April 2018 DOI: https://doi.org/10.30554/ Abstract: Objective: identify the vaginal colonizing Candida species and VVC species, archmed.18.1.2010.2018 predisposing factors and susceptibility against fluconazole in pregnant women attending Redalyc: https://www.redalyc.org/ gynecological outpatient of a maternal clinic in Cartagena (Colombia). -
Candida Auris: a Decade of Understanding of an Enigmatic Pathogenic Yeast
Journal of Fungi Review Candida auris: A Decade of Understanding of an Enigmatic Pathogenic Yeast Ryan Kean 1, Jason Brown 2 , Dolunay Gulmez 2,3 , Alicia Ware 1 and Gordon Ramage 2,* 1 Department of Biological and Biomedical Sciences, School of Health and Life Sciences, Glasgow Caledonian University, Glasgow G4 0BA, UK; [email protected] (R.K.); [email protected] (A.W.) 2 School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, Glasgow G2 3JZ, UK; [email protected] (J.B.); [email protected] (D.G.) 3 Medical Microbiology Department, Faculty of Medicine, Hacettepe University, Ankara 06230, Turkey * Correspondence: [email protected]; Tel.: +44(0)141 211 9752 Received: 13 January 2020; Accepted: 22 February 2020; Published: 26 February 2020 Abstract: Candida auris is an enigmatic yeast that continues to stimulate interest within the mycology community due its rapid and simultaneous emergence of distinct clades. In the last decade, almost 400 manuscripts have contributed to our understanding of this pathogenic yeast. With dynamic epidemiology, elevated resistance levels and an indication of conserved and unique pathogenic traits, it is unsurprising that it continues to cause clinical concern. This mini-review aims to summarise some of the key attributes of this remarkable pathogenic yeast. Keywords: Candida auris; pathogenicity; in vivo models; biofilms 1. Introduction A decade on since its discovery, Candida auris has rapidly emerged as a significant nosocomial pathogen, responsible for an escalating number of infections across the globe. C. auris possesses some almost unique characteristics for an infectious yeast in that it can survive and persist within the environment for prolonged periods and a significant number of clinical isolates have been reported to be multi-drug resistant, with a very small proportion resistant to three classes of antifungals [1]. -
The Evolving Landscape of Fungal Diagnostics, Current and Emerging Microbiological Approaches
Journal of Fungi Review The Evolving Landscape of Fungal Diagnostics, Current and Emerging Microbiological Approaches Zoe Freeman Weiss 1,2,*, Armando Leon 1 and Sophia Koo 1 1 Brigham and Women’s Hospital, Division of Infectious Diseases, Boston, MA 02115, USA; [email protected] (A.L.); [email protected] (S.K.) 2 Massachusetts General Hospital, Division of Infectious Diseases, Boston, MA 02115, USA * Correspondence: [email protected] Abstract: Invasive fungal infections are increasingly recognized in immunocompromised hosts. Current diagnostic techniques are limited by low sensitivity and prolonged turnaround times. We review emerging diagnostic technologies and platforms for diagnosing the clinically invasive disease caused by Candida, Aspergillus, and Mucorales. Keywords: fungal diagnostics; mycoses; invasive candidiasis; invasive mold infections; invasive aspergillosis; mucormycosis; transplant; immunocompromised host; non-culture diagnostics; cul- ture independent 1. Introduction In recent years, the incidence of invasive fungal infections has increased in parallel with advances in chemotherapies, immunosuppression in solid organ and hematopoietic cell transplantation, and critical care technologies. The diagnosis of invasive fungal disease Citation: Freeman Weiss, Z.; Leon, has traditionally relied on culture, direct microscopy, and histopathology. Conventional A.; Koo, S. The Evolving Landscape culture techniques are frequently insensitive, have prolonged turnaround times (TAT), of Fungal Diagnostics, Current and and may require invasive sampling. An increase in the diversity of pathogenic species Emerging Microbiological makes phenotypic identification challenging, particularly as the number of skilled clinical Approaches. J. Fungi 2021, 7, 127. mycologists declines. Precise species identification is needed given the variability of https://doi.org/10.3390/jof7020127 antifungal drug susceptibility profiles even between closely related organisms.