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Transcriptome-wide analysis of the response of the thecosome pteropod Clio pyramidata to short-term CO2 exposure
Article in Comparative Biochemistry and Physiology Part D Genomics and Proteomics · June 2015 DOI: 10.1016/j.cbd.2015.06.002 · Source: PubMed
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AE Maas Ann M Tarrant Bermuda Institute of Ocean Sciences Woods Hole Oceanographic Institution
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Available from: AE Maas Retrieved on: 02 August 2016 1 Transcriptome-wide analysis of the response of the thecosome pteropod Clio pyramidata to
2 short-term CO2 exposure
3 4 5 1,2* 1 1 6 Amy E. Maas , Gareth L. Lawson , and Ann M. Tarrant 7 8 1. Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA 9 2. Current address: Bermuda Institute of Ocean Sciences, St. George’s GE01, Bermuda 10 11 *Corresponding author e-mail: [email protected].
Maas et al. 1 12 Abstract 13 Thecosome pteropods, a group of calcifying holoplanktonic molluscs, have recently become a 14 research focus due to their potential sensitivity to increased levels of anthropogenic dissolved 15 CO2 in seawater and the accompanying ocean acidification. Some populations, however, already 16 experience high CO2 in their natural distribution during diel vertical migrations. To achieve a 17 better understanding of the mechanisms of pteropod calcification and physiological response to 18 this sort of short duration CO2 exposure, we characterized the gene complement of Clio 19 pyramidata, a cosmopolitan diel migratory thecosome, and investigated its transcriptomic 20 response to experimentally manipulated CO2 conditions. Individuals were sampled from the 21 Northwest Atlantic in the fall of 2011 and exposed to ambient conditions (~380 ppm) and 22 elevated CO2 (~800 ppm, similar to levels experienced during a diel vertical migration) for ~10 23 hrs. Following this exposure the respiration rate of the individuals was measured. We then 24 performed RNA-seq analysis, assembled the C. pyramidata transcriptome de novo, annotated the 25 genes, and assessed the differential gene expression patterns in response to exposure to elevated 26 CO2. Within the transcriptome, we identified homologs of genes with known roles in 27 biomineralization in other molluscs, including perlucin, calmodulin, dermatopontin, calponin, 28 and chitin synthases. Respiration rate was not affected by short-term exposure to CO2. Gene 29 expression varied greatly among individuals, and comparison between treatments indicated that 30 C. pyramidata down-regulated a small number of genes associated with aerobic metabolism and 31 up-regulated genes that may be associated with biomineralization, particularly collagens and C- 32 type lectins. These results provide initial insight into the effects of short term CO2 exposure on 33 these important planktonic open-ocean calcifiers, pairing respiration rate and the gene expression 34 level of response, and reveal candidate genes for future ecophysiological, biomaterial and 35 phylogenetic studies. 36 37 38 39 40 41 42 43 44 45 46 47 48 49 Keywords: acidification, climate change, gastropod, lectin, mollusc, RNA-seq, transcriptomics
Maas et al. 2 50 Introduction 51 The dissolution of anthropogenic carbon dioxide (CO2) into seawater, or ocean acidification 52 (OA), stands to impact a broad variety of marine organisms, especially those that secrete calcium 53 carbonate (CaCO3) shells or skeletons (Fabry et al., 2008). As CO2 equilibrates into seawater, pH 54 and the concentration of carbonate ions decrease, causing CaCO3 to dissociate and affecting the 55 ability of calcifying animals to create and maintain calcareous structures (Gattuso et al., 1999; 56 Riebesell et al., 2000). Furthermore, decreasing pH in the water alters the acid-base balance of 57 the intra- and extracellular fluids of marine organisms (Miles et al., 2007; Seibel and Fabry, 58 2003; Seibel and Walsh, 2001). Since CO2 is produced naturally as a byproduct of respiration, all 59 organisms have physiological mechanisms for maintaining internal cellular pH. These 60 compensatory mechanisms may, however, cease to function if internal acidosis becomes too 61 severe over short time scales and generally fail over extended periods of internal pH imbalance. 62 Classical organismal-level metrics (e.g., metabolic rate, calcification, mortality) have revealed a 63 complex pattern of intra- and inter-specific variation in responses to CO2 exposure (reviewed in 64 Hendriks et al., 2010; Kroeker et al., 2013; Kroeker et al., 2010). The variability in these 65 responses, and their dependence on duration of exposure or co-varying stressors, suggest that 66 there is an energetic cost associated with compensating for high CO2 (e.g.,69 Cummings et al., 67 2011; Langenbuch and Pörtner, 2004; Stumpp et al., 2011b). 7068 71 One group thought to be particularly sensitive to OA is the thecosomatous (i.e., shelled) 72 pteropods. These holoplanktonic gastropods, related to terrestrial snails and also known as sea 73 butterflies, produce thin shells made of aragonite, a highly soluble form of CaCO3. In temperate 74 and polar seas, thecosomes can become a numerically dominant member of the zooplankton 75 community (Hunt et al., 2008). This causes them to be significant consumers of primary 76 production, serve as a key food item for commercially important fish, seabirds and whales, and 77 substantially contribute to carbon flux to the deep ocean (reviewed in: Bednaršek et al., 2012a; 78 Lalli and Gilmer, 1989; Manno et al., 2010). Studies of thecosome biomineralization have 79 revealed that increasing CO2 levels result in decreased calcification and shell degradation 80 (Bednaršek et al., 2014; Bednaršek et al., 2012b; Comeau et al., 2009; Lischka and Riebesell, 81 2012; Manno et al., 2012). It remains unclear whether the decreases in calcification documented 82 in thecosomes at high CO2 concentrations are a result of their inability to form carbonates, or 83 rather are a result of a re-allocation of energy, as has been described in other species (e.g., 84 Melzner et al., 2013; Stumpp et al., 2012). 85 86 87 The impact of CO2 on the metabolic rate and mortality of thecosome pteropods has been shown 88 to be variable, and highly dependent upon the presence of co-varying stressors such as salinity, 89 temperature and feeding history (Comeau et al., 2010a; Lischka and Riebesell, 2012; Maas et al., 90 in review; Manno et al., 2012; Seibel et al., 2012). In contrast to laboratory findings concerning 91 the effects of under-saturation on pteropods, Maas et al. (2012) found that some species of 92 thecosomes regularly experience conditions of high CO2 and aragonite saturation states <1 93 during diel vertical migrations into an oxygen minimum zone in the eastern tropical North
Maas et al. 3 94 Pacific, and do not respond to short-term laboratory CO2 exposure with a change in metabolic 95 rate. This suggests that in some thecosome species there is a potential adaptation or acclimation 96 to high CO2, at least over short periods. Supporting this idea, more recent work comparing 97 thecosomes from the Northeast Pacific and Northwest Atlantic revealed that the metabolic rates 98 of multiple thecosome pteropod species are not affected by short-term exposure to CO2 at the 99 levels predicted for the coming century (800 ppm; Maas et al., in review). Exploring the 100 physiological mechanisms these species use to cope with short-term CO2 exposure may provide 101 insight into what duration and level of exposure to anthropogenic CO2 thecosomes can tolerate. 102 Elucidating these mechanisms will also provide a means to characterize the physiological 103 condition of pteropods within natural populations and enable development of molecular 104 biomarkers indicating responses to elevated CO2 exposure. 105 106 107 An increasing number of studies have employed molecular methods to disentangle the complex 108 picture associated with CO2 response, revealing changes in expression of genes with a known or 109 predicted role in acid-base balance, apoptosis, biomineralization, development, protein synthesis, 110 energetic metabolism, and stress responses (Hüning et al., 2013; Kaniewska et al., 2012; 111 Kurihara et al., 2012; Moya et al., 2012). For example, Moya et al. (2012) showed that coral 112 larvae respond to CO2 through changes in gene expression that were consistent with suppressed 113 metabolism and changes in calcification. They also discovered an unexpected lack of response in 114 ion transport proteins and a down-regulation of carbonic anhydrase genes. Relatively few studies 115 have matched observations of altered gene expression with organismal-level observations such 116 as developmental delays or changes in metabolic rate (e.g., Stumpp et al., 2011b). Many of the 117 previous studies have used quantitative PCR (qPCR), a targeted approach which involves 118 picking a small number of genes a priori such as studies with mussel (Hüning et al., 2013), 119 abalone (Zippay and Hofmann, 2010), urchin (Stumpp et al., 2011a), and oyster (Liu et al., 120 2012), or using microarrays, which have historically only been done with well-studied organisms 121 such as urchins (Evans et al., 2013; O'Donnell et al., 2010; Todgham and Hofmann, 2009) and 122 corals (Kaniewska et al., 2012). 123 124 125 Despite the benefits of using gene expression studies for understanding the response to CO2 at a 126 mechanistic level, there have been no published studies of pteropod gene expression to date. 127 High throughput RNA sequencing (RNA-seq) methods enable quantitative characterization of 128 gene expression patterns across the transcriptome (Ekblom and Galindo, 2011; Vijay et al., 129 2012). When a reference genome is not available, a transcriptome can be assembled de novo 130 from millions of short reads. The reads from individual samples can then be mapped onto this 131 reference transcriptome for a comprehensive assessment of differential gene expression. 132 Furthermore, once a transcriptome has been assembled, it can serve as a resource for targeted 133 analyses of individual genes. In the context of ocean acidification, these targeted analyses may 134 include the identification of pteropod homologs of genes associated with calcification in other 135 mollusc species.
Maas et al. 4 136 137 A number of recent papers have focused on the characterization of biomineralization proteins 138 and genes in molluscs (Gardner et al., 2011; Jackson et al., 2010; Joubert et al., 2010; Shi et al., 139 2012; Zhang and Zhang, 2006; Zhao et al., 2012a), while other studies have increased the number 140 of available molluscan transcriptomes to include pearl oysters (Huang et al., 2012; Zhao et al., 141 2012a; Zhao et al., 2012b), sea hares (Fiedler et al., 2010; Heyland et al., 2011), freshwater snails 142 (Sadamoto et al., 2012), abalone (Franchini et al., 2011; Picone et al., 2015), scallops (Artigaud 143 et al., 2014), mussels (Freer et al., 2014), and clams (Clark et al., 2010; Sleight et al., 144 2015). These studies confirm that molluscan biomineralization is a complex process with some 145 conserved pathways shared between distantly related animal groups, and other proteins and 146 compounds that are taxonomically restricted (Jackson et al., 2010), as has been shown in more 147 distantly related animal groups (Jackson et al., 2007; Moya et al., 2012; Moya et al., 2008). 148 Comparing the transcriptomic underpinnings of shell accretion in thecosomes with those in other 149 molluscs may thus provide a useful contrast because of the different ecological pressures of a 150 holoplanktonic lifestyle and the structures and processes associated with building a calcium 151 carbonate shell principally (> 50%) comprised of aragonite (Bé and Gilmer, 1977). Beyond 152 understanding the mechanism of calcification to explore the specific effects of climate change on 153 shell formation, biomineralization in the molluscan lineage may thus also be interesting from a 154 material properties and evolutionary standpoint (Jackson et al., 2010; Xie et al., 2011). 155 156 157 In this study, we focus on Clio pyramidata (Linnaeus, 1767), a common circumglobal species 158 that has a multilayered aragonite shell, and is known to migrate vertically on a diel basis into 159 regions of high CO2 in some ocean basins (Maas et al., 2012). As in most thecosomes, the 160 growth sequence and processes of biomineralization for this species are poorly-characterized and 161 no gene sequences beyond the barcoding regions were known. In this study, we have assembled 162 and annotated a de novo transcriptome for C. pyramidata. To characterize the transcriptome, and 163 to gain insight into the effects of short-term CO2 exposure (similar to what would be experienced 164 during a diel migration), we then exposed C. pyramidata to ambient or elevated CO2 165 concentrations, measured respiration rate, and used RNA-seq to test for changes in gene 166 expression. By pairing these two metrics we hoped to shed light on the lack of a significant effect 167 of CO2 exposure on C. pyramidata oxygen consumption rates that has been observed previously 168 in similar metabolic experiments in the Pacific (Maas et al., 2012). Specifically, transcriptional 169 profiling was used to investigate whether this lack of response to short term CO2 exposure is due 170 to an absence of physiological stress, or is rather a product of the redistribution of energetic 171 resources. 172 173 174 Materials and Methods: 175 Animal Collection and Experimental Setup: 176 C. pyramidata were collected from the R/V Oceanus in August 2011 at an off-shelf station east 177 of the Grand Banks in the Northwest Atlantic (44.94°N, 42.00°W). Animals were captured using
Maas et al. 5 178 a 1 m diameter, 150 µm mesh, Reeve net. Clio pyramidata are small, relatively rare, difficult to 179 capture without damaging, and hard to maintain in shipboard conditions, limiting the numbers 180 available for experimentation. Individuals for this experiment were collected from the same net 181 tow to minimize pre-existing environmental variability and handling differences between tows 182 (hydrographic data and tow details in Supplementary File 1). Clio pyramidata from this tow 183 were placed in open jars of locally collected filtered seawater (33 psu) at 15°C in temperature- 184 controlled waterbaths at densities < 15 individuals L-1 for 12 h (± 30 min) to allow for gut 185 clearance. This temperature and salinity were chosen as individuals of this species had recently 186 been sampled under these conditions. 187 188 189 CO2 Exposures and Respiration Experiments 190 After the gut clearance period, healthy individuals (swimming and with intact shells) were 191 selected for closed chambered respiration experiments using a Clarke-type oxygen electrode 192 method (Maas et al., 2012; Marsh and Manahan, 1999). Individuals were placed in separate 20 193 mL air-tight glass chambers with 0.2 micron filtered seawater. The experimental water (33 psu) 194 had previously been bubbled in 1 L batches for 45-60 minutes with ambient air (~380 ppm CO2) 195 or a certified gas mixture containing 800 ppm CO2 (± 2 %, Corp Brothers; 21% oxygen, 196 balanced with nitrogen). This treatment was applied to simulate projected levels for the open 197 ocean in the year 2100 (A2 emissions scenario, I.P.C.C., 2007) and is a level that C. pyramidata 198 is known to currently experience in other portions of its distribution (i.e., the North Pacific). The 199 water contained 25 mg L-1 each of streptomycin and ampicillin to prevent bacterial growth, a 200 procedure that has been shown to have no effect on the respiration rate of thecosome pteropods 201 (Howes et al., 2014). 202 203 204 For every 3 experimental glass chambers, another control glass chamber was set up with the 205 same water but without an animal to enable monitoring of background bacterial respiration. 206 Respiration chambers were maintained at temperature in a waterbath during experimental 207 incubations, after which the oxygen saturation in the chamber was measured. Duration of 208 exposure varied slightly due to the sequential measurement of O2 concentration in successive 209 chambers at the beginning and end of the experiments, but CO2 exposures were between 9.5-10 210 hours for the high treatment and 10.25-10.75 h for the ambient treatment (to replicate diel 211 vertical migratory periods). For each measurement, an aliquot of water was withdrawn using an 212 airtight 500 μL Hamilton syringe and injected past a Clarke-type microcathode (part #1302, 213 Strathkelvin Instruments, North Lanarkshire, United Kingdom), which was attached to an O2 214 meter (part #782) in a water-jacketed injection port (part #MC100), and the reading was allowed 215 to stabilize for at least 30 seconds prior to being recorded. At the conclusion of the experiments, 216 oxygen in the chambers remained between 73-96% of saturation, above the critical oxygen 217 partial pressure typical of marine animals (Childress and Seibel 1998). The animals were then 218 removed from the chamber, blotted dry, and frozen in liquid nitrogen. Total handling time of
Maas et al. 6 219 organisms during the final O2 measurements was ~10 min per respiration experiment, with less 220 than a minute between removal from the chamber and preservation to minimize handling stress. 221 222 223 The same individuals used in the study of oxygen consumption were stored at -80°C for 224 transcriptomic analysis. Prior to RNA extraction, individuals were quickly weighed on a 225 microbalance (+/- 0.0001 g) to allow for the calculation of their mass specific O2 consumption 226 rate (µmoles g-1 h-1 wet weight). Tissue was then dissected from the shell to avoid both the 227 possible pH interference due to the calcium carbonate of the structure and contamination by 228 other taxa trapped in the shell. Statistical analysis of respiration rate was conducted using a 229 general linear model in SPSS on log-transformed oxygen consumption, with treatment as the 230 random factor and log-transformed wet mass as a covariate. 231 232 233 Carbonate Chemistry 234 DIC measurements were taken after each experiment from control syringes that had been allowed 235 to come to room temperature (>6 h) before analysis. DIC samples were analyzed on a DIC auto- 236 analyzer (AS-C3, Apollo SciTech, Bogart, USA) via acidification, followed by non- dispersive 237 infrared CO2 detection (LiCOR 7000: Wang et al., 2013a; Wang and Cai, 2004). Samples were 238 measured a minimum of three times to obtain replicate measurements. The instrument was 239 calibrated with certified reference material (CRM) from Dr. A.G. Dickson at the Scripps 240 Institution of Oceanography and has a precision and accuracy of ± 2.0 µmol kg-1. Best practices 241 for calculating carbonate chemistry (Riebesell et al., 2010) include measurement of two of the 242 CO2 system parameters. Due to the small volumes of water in the experimental chambers 243 it was not possible to measure both DIC and total alkalinity simultaneously from the control 244 syringes, and instead total alkalinity of batches of water (collected weekly) was measured using 245 an Apollo SciTech alkalinity auto-titrator, a Ross combination pH electrode, and a pH meter 246 (ORION 3 Star) based on a modified Gran titration method (Wang et al., 2013b). These batches 247 of water were then used for multiple experiments over the course of several days. Unfortunately, 248 the alkalinity changed over time, likely due to microbial activity, and measurements proved not 249 to have been taken at sufficient temporal resolution to constrain the carbonate chemistry within 250 this experiment using total alkalinity. Subsequent diffusion calculations, however, indicated that 251 the 45 - 60 min bubbling was sufficient to fully achieve the targeted pCO2 levels. As such we 252 used the manufacturer’s certification of pCO2 within the gas mixture as our second parameter to 253 characterize the carbonate system. Other carbonate system parameters, including aragonite 254 saturation state, were calculated using the CO2SYS software (Pierrot et al., 2006), the carbonic 255 acid dissociation constants by Mehrbach et al. (1973), refitted by Dickson and Millero (1987), 256 and the KHSO4 dissociation constant from Dickson (1990). The ± 2% error in CO2 concentration 257 reported by the gas supplier was incorporated into the calculations of the carbonate chemistry to 258 characterize uncertainty. 259 260 RNA Extraction and Illumina Sequencing
Maas et al. 7 261 RNA was extracted from individuals with either the Aurum Total RNA Fatty and Fibrous Tissue 262 Kit (BioRad) or the E.Z.N.A. Mollusc RNA Kit (Omega Biotek) and eluted in 25-40 µL 263 molecular biology grade water. Both methods yielded one sharp band of RNA with no signs of 264 degradation on a denaturing agarose gel and on a Bioanalyzer profile. This indicates that 265 pteropods, like some other protostomes, likely have a delicate 28S ribosomal RNA strand that 266 breaks during extraction, making a traditional quality score relatively uninformative (Gayral et 267 al., 2011; Winnebeck et al., 2010). 268 269 270 RNA extracts from four individuals per treatment (8 individuals in total, A-D exposed to 271 elevated CO2, E-H exposed to ambient) were chosen based on RNA quality and yield. RNA was 272 submitted to the Genomic Sequences Laboratory of Hudson Alpha for library construction and 273 sequencing. Libraries were constructed using Tru-Seq RNA Sample Prep Kits, multiplexed, and 274 sequenced in duplicate on two lanes of the Illumina HiSeq platform (v. 1.9) as 50 base pair (bp) 275 paired-end reads. 276 277 278 Assembly and Annotation 279 Raw sequences were quality filtered and assembled into transcripts in silico. The resulting 280 transcripts were annotated to indicate their similarity to genes with known function. To 281 accomplish this, adapter sequences and bases with a phred quality score below 30 were first 282 removed using Trimmomatic (Lohse et al., 2012). De novo transcriptome assembly was 283 performed with Trinity (version r2013-02-25: Grabherr et al., 2011) using a pooled file of all 284 reads from both treatments and the default parameters (minimum contig length: 200, k-mer 285 length 25). The assembled transcripts were imported into Blast2GO (Conesa et al., 2005), where 286 those > 200 bp were compared to the SWISS-PROT database using BLASTX with an e-value 287 <1.0 e-6 to remain relatively consistent with other recent non-model marine transcriptomes (i.e., 288 Burns et al., 2013; Craft et al., 2010; de Wit and Palumbi, 2013; Harms et al., 2013). These 289 transcripts were further annotated using the Blast2GO program that assigned Gene Ontology 290 (GO) terms for those with an e-value <1.0 e-10 to reduce the probability of mis-annotation (du 291 Plessis et al., 2011). 292 293 294 Analysis of Biomineralization Genes 295 To specifically search for transcripts associated with biomineralization, we utilized the recent 296 annotation of the oyster genome, which includes both a set of genes that were either highly or 297 differentially expressed in shell mantle tissue and a comparison of oyster genes with sequences 298 known to be associated with shells in other molluscs (Zhang et al., 2012). We assembled a 299 BLAST database using the Genbank accession numbers for these mantle and biomineralization 300 proteins. We then conducted a TBLASTN search to find putative homologs of these genes within 301 the C. pyramidata transcriptome (e-value <1.0 e-6). Transcripts of interest, such as those that 302 showed homology to perlucin sequences in the molluscan biomineralization database were again 303 used to query the NCBI non-redundant database (nr) using BLASTX as implemented in
Maas et al. 8 304 Blast2GO. The sequences exhibiting greatest similarity to annotated perlucin sequences (five 305 sequences with lowest e-values) were translated in silico using the ExPASy translate tool 306 (Artimo et al., 2012). Stop codons were trimmed, and then these sequences were aligned with 307 annotated perlucin protein sequences from other molluscs using MUSCLE (Edgar, 2004). These 308 included the perlucin sequences that have been shown to be associated with biomineralization in 309 the triangle sail mussel (Hyriopsis cumingii, GenBank KC436008: Lin et al., 2013), the abalone 310 (Haliotis laevigata, P82596.3: Mann et al., 2000), and from the disk abalone (Haliotis discus 311 discus, ABO26590.01: Wang et al., 2008). Also included in the alignment was the C-type lectin 312 from the bay scallop that appears to be associated with immune response (Argopecten irradians, 313 ADL27440.1 Mu et al., 2012), as well as sequences inferred in silico such as the perlucin 314 sequences associated with the Pacific oyster (Crassostrea gigas, EKC39512.1: Zhang et al., 315 2012) and the abalone (Haliotis diversicolor, AEQ16377.1) and the C-type lectins from the pearl 316 oyster (Pinctada fucata, ACO36046.1) and the clam (Ruditapes philippinarum, ACU83213). 317 318 319 Differential Expression 320 The reads from each library were pooled and separately mapped to our reference transcriptome 321 using Bowtie (Langmead et al., 2009) via the RSEM script that is associated with the Trinity 322 package set with default parameters (Haas et al., 2013). To compare overall gene expression 323 patterns among individuals prior to identifying differentially expressed genes, we used the 324 statistical package Primer (Clarke and Gorley, 2006) to construct a Bray-Curtis similarity matrix 325 of the standardized edgeR normalized (TMM, trimmed mean of M-values; Robinson and 326 Oshlack, 2010) fragments per kilobase per million reads mapped (FPKM) transcript counts of all 327 genes (Trinity components) expressed in at least two individuals (to reduce variability caused by 328 rare transcripts). The edgeR package was then used to identify genes (Trinity components) that 329 exhibited significant differences in expression between the two CO2 treatments (Robinson et al., 330 2010). Results were reported as significant when the change in log2-transformed gene expression 331 was greater than two (corresponding to a four-fold change in expression) and the p-value and 332 false discovery rate were < 0.05. These differentially expressed genes were re-annotated using a 333 BLASTX against the NCBI non-redundant (e-value <1.0 e-3) and Interpro (Hunter et al., 2011) 334 databases. 335 336 337 Results 338 Consistent with experiments conducted in other ocean basins (Maas et al., in review; Maas et al., 339 2012), wild caught C. pyramidata from the Northwest Atlantic that were exposed to either 340 ambient (~380 ppm) or elevated (~800 ppm) CO2 for ~10 h, simulating the timing of exposure 341 associated with diel vertical migration, showed no significant difference in respiration rate. 342 Using these same experimental individuals, libraries were sequenced from eight pteropods (4 per 343 treatment) using Illumina HiSeq technology. These libraries were used to build a reference 344 transcriptome for C. pyramidata that revealed a number of biomineralization-associated genes 345 similar to those found in other molluscan lineages. Only a small number of genes exhibited
Maas et al. 9 346 significant changes in expression in association with short-term exposure to moderately elevated 347 CO2. 348 349 350 Respiration Experiments and Carbonate Chemistry 351 The experimental CO2 exposure achieved an aragonite saturation state (ΩAr) of 2.44 and pH of 352 8.06 in the ambient treatment (380 ppm CO2) and an ΩAr of 1.41 and pH of 7.78 (800 ppm CO2) 353 in the elevated treatment (Table 1). Incorporating the uncertainty in pCO2 based on the 354 manufacturer’s certification of the gas mixture, we calculated a potential error of ± 0.03 in ΩAr 355 and ± 0.01 in pH for the 800 ppm treatment; thus our treatments were near the targeted values 356 and achieved the desired result of acutely different exposure conditions. There were no 357 significant differences in the respiration rate of individuals from the two treatments; this also 358 remained true when considering a larger sample size of individuals from more sample locations 359 in the Atlantic as well as from the Pacific Ocean (Fig. 1). There were no significant differences 360 between the treatments within the Atlantic (F1,14 = 0.037, p =0.850), nor when individuals from 361 the Pacific were included in the analysis (F1,42 = 0.540, p =0.466). 362 363 364 Assembly and Annotation 365 Sequencing resulted in ~50 million reads per individual after quality trimming. The assembled 366 transcriptome contained 30,800 components (“genes”) and 45,739 transcripts (“isoforms”) 367 greater than 200 bp, an average transcript length of 618 bp, and an N50 of 852 bp. We were able 368 to annotate 9,280 of the transcripts (20%) based on similarity to genes within the SWISS-PROT 369 database using an e-value cut-off of 1.0 e-6. The percent of annotated sequences was positively 370 correlated with transcript length; however, we were still unable to identify 70% of the longest 371 sequences (>2,000 bp). Gene ontology (GO) terms were assigned to 7,989 of the transcripts with 372 significant BLAST hits (86%). 373 374 375 Candidate biomineralization-associated transcripts 376 A number of genes that have previously been shown to be involved with biomineralization have 377 potential homologs within the pteropod transcriptome. Queries of the pteropod transcriptome 378 with 45 biomineralization-associated genes from other molluscs resulted in identification of 831 379 C. pyramidata transcripts. These included 13 transcripts that were annotated as carbonic 380 anhydrase precursors, 59 as calmodulin or calmodulin-like proteins, 10 chitin synthases, and 381 surprisingly 282 transcripts annotated as perlucin (Supplementary File 2). In addition, we 382 identified 56 transcripts through queries with 23 sequences that were up-regulated in oyster 383 mantle (Zhang et al., 2012). Transcripts that showed homology to perlucin sequences in the 384 molluscan biomineralization database were BLASTed against the nr database to help provide a 385 list of the most likely perlucin candidates. When translated, the top five matches (based on e- 386 value) aligned well with previously identified perlucin transcripts from various other molluscs 387 (Fig. 3), revealing the six highly conserved cystine residues, the residues putatively involved in 388 calcium-dependent carbohydrate binding (Mann et al., 2000) and the motifs proposed to
Maas et al. 10 389 determine carbohydrate-binding specificity for galactose (QPD) binding (Iobst and Drickamer, 390 1994). 391 392 393 Differential expression 394 There was a high degree of variability in overall transcript expression patterns among individuals 395 (20-80% similarity in pairwise comparisons, Fig. 2). Between treatments 29 genes were 396 differentially expressed, four of which were down-regulated in high CO2 and the rest of which 397 were up-regulated (Table 2). Of the four genes that were down-regulated in individuals exposed 398 to high CO2, two were annotated: both were associated with the mitochondrial inner-membrane 399 complex (cytochrome c oxidase subunit I and III), however there was high variability in their 400 expression levels within treatments. Of the 29 genes that were up-regulated only six could be 401 annotated through BLASTX searches of the NCBI non-redundant database and an InterPro Scan. 402 Two of these were associated with carbohydrate binding of the C-type lectin family (conglutinin 403 and a lactose-binding lectin that was also identified as being a perlucin homolog), one was 404 associated with protein binding, another with the regulation of transcription via DNA binding, 405 another with hydrolase activity in an extracellular region, and the final was a chymotrypsin-like 406 elastase (Table 2). 407 408 409 Discussion 410 The sequences generated by this de novo transcriptome assembly provide a first glimpse into the 411 gene complement of a thecosome pteropod, revealing a number of transcripts that are putative 412 homologs of genes involved in biomineralization in other molluscs. The respiration rate 413 experiments show no effect of short term CO2 exposure, a finding that is also consistent with the 414 very small number of candidate genes that were identified in the differential expression analysis. 415 The candidate genes that may respond to pH conditions replicating exposures and durations 416 comparable to a diel vertical migration include those associated with biomineralization and 417 aerobic metabolism, although these results should be interpreted cautiously and require 418 validation with a larger dataset. 419 420 421 Assembly and Annotation 422 Comparison of the de novo thecosome pteropod transcriptome with the SWISS-PROT database 423 resulted in annotation of ~20% of the 45,739 transcripts. This relatively low percentage of 424 identified transcripts is within the range of other recent assemblies of transcriptomes from non- 425 model marine invertebrates, all of which were annotated using the NCBI non-redundant database 426 (19-37%, spider crab (Harms et al., 2013), coral (Meyer et al., 2011), krill (Clark et al., 2011), 427 amphipod (Zeng et al., 2011), abalone (de Wit and Palumbi, 2013), brittle star (Burns et al., 428 2013). Use of SWISS-PROT, a highly-curated and more restricted database, rather than the 429 NCBI non-redundant database, which has greater species coverage, was selected to achieve more 430 informative annotation. Thus, as in other studies, low identification is likely due to the relatively
Maas et al. 11 431 small available database of annotated genes from closely-related species, typical in the study of 432 non-model organisms. 433 434 435 Biomineralization 436 Queries of the pteropod transcriptome with bivalve biomineralization transcripts revealed a large 437 number of potential homologs including dermatopontin, calmodulin, calponin, chitin synthases 438 and carbonic anhydrase precursors, providing a useful resource for future exploration of these 439 gene families. There were also a number of genes for which the annotation corresponded to as- 440 yet undescribed transcripts associated with shell mantle expression in oyster. The highest number 441 of transcripts found via our search, however, belonged to a group of C-type lectin domain 442 proteins that are similar to perlucin. We identified five likely perlucin homologs, which would be 443 the best first choices to further explore this gene family in pteropods. Perlucin, a water-soluble 444 non-acidic protein with a calcium-dependent carbohydrate binding domain has been shown to be 445 important for shell biomineralization in the nacreous (aragonitic) layer of the abalone Haliotis 446 laevigata (Mann et al., 2000), and in the freshwater pearl mussel Hyriopsis cumingii (Lin et al., 447 2013). Perlucin-like genes have also been found to be strongly expressed in the outer mantle 448 tissue of the mussel Mytilus edulis (Freer et al., 2014; Hüning et al., 2012). The abalone perlucin 449 protein has been described through functional studies as a promoter of calcium carbonate 450 precipitation, as the site of nucleation for calcium carbonate crystallization, and as a modifier of 451 crystal morphology (Blank et al., 2003; Weiss et al., 2000). A recombinant perlucin gene from 452 the abalone Haliotis discus discus has been shown to promote the growth of calcium carbonate 453 crystals in E. coli (Wang et al., 2010). The C-type lectin domain proteins also can have an 454 immune function in molluscs (Mu et al., 2012; Yang et al., 2011), and it has been hypothesized 455 that some perlucin-like sequences may have an immune function (Venier et al., 2011; Wang et 456 al., 2008). If the pteropod perlucin-like sequences are associated with shell healing or repair, 457 their induction could be an important physiological pathway for pteropods in the response to 458 high CO2. 459 460 461 Interestingly, as was found during annotation of the oyster genome, a number of different 462 transcripts were frequently identified as putative homologs of a single sequence. This was 463 particularly evident for perlucin, where all 282 genes were identified as being homologs of only 464 4 abalone sequences, each which were described as either perlucin or perlucin-like genes. Since 465 the perlucin-like C-type lectins appear to have diversified within the lineage leading to C. 466 pyramidata, further investigation of the evolution of C-type lectins within pteropods should be a 467 priority for further study. Similarly, the large number of paralogous biomineralization genes in 468 the oyster genome was suggested by Zhang et al. (2012) to be a consequence of rapid 469 diversification and strong selection pressure, which is thought to underlie the evolutionary 470 history of shell related genes. It is interesting that rapid diversification may have occurred 471 independently in the highly derived holoplanktonic pteropods, emphasizing the usefulness of this 472 species for comparative studies of biomineralization.
Maas et al. 12 473 474 Differential Expression 475 We found high variability in gene expression among individual pteropods, likely due to the fact 476 that samples were generated from wild-caught individuals, which may have included differences 477 in genetic background, physiological condition and size. It is also important to note that, because 478 these are small animals, analysis was done with whole organisms, rather than specific tissues, 479 which may dampen signals from specialized tissues such as the biomineralizing mantle. In the 480 context of this high variability, the limitation of whole animal extractions, and the low number of 481 biological replicates, the small subset of genes that were differentially expressed as a 482 consequence of exposure to high CO2 should be considered with caution and requires further 483 confirmation, including further exploration of gene expression patterns with qPCR. Due to the 484 rarity and small size of the organisms, we did not have enough remaining material from these 485 experiments to validate our findings concerning differential expression reliably through qPCR 486 measurements. In combination with a lack of effect on respiration rate (Maas et al., in review; 487 Maas et al., 2012), however, the low number of differentially expressed genes suggests a lack of 488 substantial physiological response to the experimental conditions. 489 490 491 Despite the limitations of the differential expression analyses, the present results nonetheless 492 provide insight into transcripts that may be potentially influenced by short duration CO2 493 exposure. The small number of annotated down-regulated genes in the high CO2 treatment were 494 highly variable in expression pattern and were associated with the mitochondrial inner membrane 495 complex. Changes in mitochondrial inner membrane complex transcript abundance were also 496 observed in urchins exposed to CO2 in a longer duration developmental study (40 h) conducted 497 by Todgham and Hofmann (2009) and in coral exposed to CO2 for three days by Moya et al. 498 (2012) and interpreted as being associated with a reduction in oxidative metabolism. Subunits of 499 the downstream enzyme in the oxidative metabolism pathway, ATP synthase, have also been 500 documented to be down-regulated in invertebrate models (mussels, Hüning et al., 2013; urchins, 501 Todgham and Hofmann, 2009), emphasizing oxidative metabolism as an important molecular 502 response to high CO2. Those genes that were up-regulated had annotations that corresponded 503 with the extracellular region and gene families known to be associated with biomineralization. 504 These included putative homologs of perlucin, and conglutinin, which is a calcium-dependent 505 collagen-containing lectin. Both conglutin and perlucin are C-type lectins. This diverse family is 506 known to be associated both with innate immune response and with the biomineralization protein 507 matrix (Mann et al., 2000; Mu et al., 2012; Wang et al., 2008). Moya et al. (2012) also found up- 508 regulation of collagens and lectins in coral that had been exposed to elevated CO2 conditions that 509 were not corrosive to CaCO3 structures (saturation state remained > 1). Other studies have seen 510 patterns of down-regulation of transcript expression of collagens in urchins (Todgham and 511 Hofmann, 2009) and both down- and up-regulation of transcript expression of C-type lectins, 512 such as SM30 in urchins (Kurihara et al., 2012; Stumpp et al., 2011a). A qPCR-based study with 513 species more closely related to pteropods (mussels) found no significant changes in a specific
Maas et al. 13 514 perlucin-like C-type lectin (Hüning et al., 2013). Clearly, the complexity and diversity of the 515 protein biomineralization matrix and its response to CO2 exposure still bears further investigation 516 across a diversity of taxa, including thecosome pteropods. 517 518 519 Conclusions 520 The species studied is a strong diel migrator that experiences conditions of high CO2 in portions 521 of its natural distribution, and was shown here to have no significant respiratory response to 522 short-term, moderate CO2 exposure. In conjunction with this lack of organismal-level response, 523 the molecular response to this degree of exposure appeared to be limited to a small portion of the 524 C. pyramidata transcriptome, with some of the identified transcripts being associated with the 525 mitochondrial inner membrane complex, carbohydrate binding and the extracellular 526 matrix/biomineralization. The analysis of biomineralization associated genes suggests a number 527 of similarities with those of other molluscan calcifiers. The C-type lectin family, and the 528 perlucin-like homologs in particular, merit further exploration both as a consequence of their 529 abundance in the transcriptome and their likely role in calcification. The sequences and potential 530 responses identified here thus pave the way for a detailed exploration of thecosome pteropod 531 biomineralization. They also provide groundwork for further experimentation with larger scale 532 experiments of individual- and population-level responses to longer term CO2 stress at the 533 molecular level, complementing the growing body of literature exploring metabolic, ecological, 534 and calcification responses of these sentinel open-ocean calcifiers. 535 536 537 Data Accessibility 538 The NCBI Bioproject number for the data associated with this project is PRJNA231010. Raw 539 sequence data was submitted to the NCBI Short Read Archive (SRA) under accession numbers 540 GSM1283048–GSM1283055. This Transcriptome Shotgun Assembly project has been 541 deposited at DDBJ/EMBL/GenBank under the accession GAWL00000000. The version 542 described in this paper is the first version, GAWL01000000. Gene expression data was 543 submitted to the NCBI Gene Expression Ombnibus (GEO) under the accession number 544 GSE53151. 545 546 547 List of Abbreviations 548 base pair (bp), Basic Local Alignment Search Tool (BLAST), calcium carbonate (CaCO3), 549 carbon dioxide (CO2), fragments per kilobase per million reads mapped (FPKM), Gene ontology 550 (GO), General Linear Model (GLM), high throughput RNA sequencing (RNA-seq), National 551 Center for Biotechnology Information (NCBI), ocean acidification (OA), quantitative 552 polymerase chain reaction (qPCR), trimmed mean of M-values (TMM) 553 554 555 Acknowledgements 556 We would like to acknowledge the Captain and crew of the R/V Oceanus, and all the scientists, 557 students and volunteers who participated in the OC473 expedition. We would also like to thank
Maas et al. 14 558 Amalia Aruda Almada, Mike Brosnahan, Adam Reitzel, Leocadio Blanco Bercial and Santiago 559 Herrera for their support, insight and input into methodologies, analysis and interpretation. The 560 Extreme Science and Engineering Discovery Environment (XSEDE), which is supported by 561 National Science Foundation grant number OCI-1053575, provided computing resources for the 562 differential expression analysis. This material is based upon work supported by the National 563 Science Foundation’s Ocean Acidification Program under grant number OCE-1041068 (to 564 Lawson, Wang, Lavery, and Wiebe), the Woods Hole Oceanographic Institution’s Access to the 565 Sea program (to Tarrant, Maas and Lawson) and the WHOI postdoctoral scholarship program (to 566 Maas). 567 568 569 Author Contributions 570 Study design was initiated by all three authors. The animal collection and experimental 571 manipulations were conducted by AEM and GLL. RNA processing and bioinformatics analysis 572 were done by AEM and AMT. The initial draft was prepared by AEM, and all co-authors 573 collaborated on data interpretation and manuscript revision.
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Maas et al. 22 869 Figure Legends: 870 871 872 Figure 1: Oxygen consumption rates (µmol g-1 h-1) of the individual pteropods used in the 873 transcriptomic study that were exposed to ambient CO2 (~380 ppm, dark red circles) and high 874 CO2 (~800 ppm, dark blue circles). There was no significant difference between the treatments 875 even when the dataset was expanded to include more individuals from the Atlantic (light colored 876 circles) and individuals from the Pacific (light colored diamonds) (datapoints from Maas et al., in 877 review). 878 879 880 Figure 2: Heat map comparing the gene expression patterns among eight individual pteropods. 881 Depicts a Bray-Curtis similarity matrix of the standardized edgeR normalized (TMM, trimmed 882 mean of M-values; Robinson and Oshlack, 2010) fragments per kilobase per million reads 883 mapped (FPKM) transcript counts for all genes that were expressed in at least two of the eight 884 study individuals. Letters A-D correspond to individuals exposed to high CO2, and letters E-H 885 correspond to individuals exposed to ambient CO2; analysis constructed without grouping 886 individuals by treatment. 887 888 889 Figure 3: Alignment of the conserved domain of the C-type lectin domain family proteins. The 890 six highly conserved cysteine residues are labeled with stars, open circles label the residues 891 putatively involved in calcium-dependent carbohydrate binding (Mann et al., 2000) and grey 892 boxes contain the motifs proposed to determine carbohydrate-binding specificity for galactose 893 (Iobst and Drickamer, 1994). Amino acid residue that consist of over 75% of the sequences are 894 documented as identical (dark grey shading) or similar (light grey shading) overlaying the 895 sequence.
Maas et al. 23 896 897 Tables: 898 Table 1: The carbonate chemistry parameters for the experiments including temperature (T.) salinity (S.), and measured dissolved 899 inorganic carbon (DIC). These were used, along with the manufacturer’s certification of the gas concentration (ppm calculation), to 900 calculate the aragonite saturation state (ΩAr) and pH of the treatments using CO2SYS. The calculations were also made incorporating 901 one standard deviation away from the nominal gas concentration (± 2%) to characterize the certainty of the treatment (ppm calculation 902 ± 2%), as well as by using the measured TA of the batch of water (2307.3; TA calculation, grey box). The TA measurements are likely 903 not representative of conditions during the actual experiments as a consequence of ongoing biological activity in the batch of water 904 between the point at which it was sampled for TA and when it was used for respiration experiments (see text). Irrespective of the 905 choice of parameters, the calculations resulted in similar estimates of the pH and ΩAr and suggest that the two treatments were 906 distinctly different. 907 908 909 Treatment T. S. DIC ± SD ppm calculation ppm calculation ± 2% 914 TA calculation -1 910 °C psu (µmol kg ) ΩAr pH pCO2 (µatm) ΩAr pH 915 pCO2 (µatm) ΩAr pH 911 380 ppm 15 33 2036.3 ± 57.9 2.44 8.06 372.4 - 387.6 2.49 - 2.40 8.07 - 8.05916 350.6 2.66 8.10 912 800 ppm 15 33 2194.9 ± 19.9 1.41 7.78 797.2 - 829.7 1.43 - 1.38 7.77 - 7.79917 830.1 1.38 7.77 913
Maas et al. 24 918 919 Table 2: Transcripts that were identified as differentially expressed (p < 0.05) in the full dataset are listed with their putative 920 annotation via BLASTX against the nr database (BLASTX ID) along with GO annotation, the e-value and percent similarity (% sim.) 921 of the transcript to the top match. Also reported is the log base 2 fold change (Log FC), log counts per million (Log CPM) and the 922 false discovery rate (FDR) of the analysis reported by edgeR. 923
% Log Log Transcript ID BLASTX ID e-value sim. FC CPM FDR GO Annotation GAWL01006673 cytochrome c oxidase subunit iii 1.90E-13 76.9 -10.34 9.88 0.010 P:aerobic electron transport chain; C:integral to membrane; C:mitochondrion; F:cytochrome-c oxidase activity GAWL01040808 cytochrome c oxidase subunit i 2.40E-22 87.8 -10.04 9.25 0.010 P:aerobic respiration; C:mitochondrial inner membrane; C:respiratory chain; F:electron carrier activity; F:iron ion binding; P:oxidative phosphorylation; F:heme binding; C:integral to membrane; P:electron
transport chain; F:cytochrome-c oxidase activity GAWL01040684 venom allergen 5-like 2.60E-23 53.8 7.07 2.27 0.040 F:hydrolase activity; C:extracellular region GAWL01040977 lactose-binding lectin l-2-like, 5.70E-14 51.0 7.27 1.31 0.041 F:carbohydrate binding BAA19861.1 (perlucin) GAWL01006926 transcription factor glial cells 9.90E-60 69.1 7.62 1.23 0.035 P:regulation of transcription, DNA-dependent; F:DNA binding missing GAWL01040816 conglutinin 1 2.10E-08 46.9 8.80 0.12 0.040 F:carbohydrate binding GAWL01040704 chymotrypsin-like elastase family 7.80E-13 52.1 11.08 0.25 0.008 P:single-organism process; P:regulation of cellular process; F:serine-type member 1-like endopeptidase activity; P:proteolysis GAWL01040711 polycystic kidney disease protein 6.30E-06 54.8 11.29 0.38 0.040 F:protein binding 1-like 2-like 924 925
926
927
928
929
930 Supplementary File 1: 931 932 The experiments were conducted as part of a larger multi-species and multi-region study (Maas 933 et al., submitted). The Reeve tow from which the animals used in this transcriptomics analysis 934 were collected was conducted on 8/21/11 at 21:16 local time, was 20 minutes in duration and 935 was made with 200 m of wire out (~150 m depth). Hydrographic parameters of the water column 936 were taken from a CTD made at the same station on the same day (Figure S1).
937 938 Figure S1: Hydrographic parameters from the site of C. pyramidata collection taken with a CTD. 939 940 941 Full dataset for respiration experiments available at BCO-DMO 942 Handle: http://hdl.handle.net/1912/6421 943 DOI: 10.1575/1912/6421 944 945 Maas, A.E., Wang, Z.A., Lawson, G.L., submitted. The metabolic response of thecosome 946 pteropods from the North Atlantic and North Pacific Oceans to high CO2 and low O2. Limnology 947 and Oceanography. 948 949
Supplementary File 2: Candidate sequences (Transcript ID) with a putative involvement in biomineralization were identified by TBLASTN with an e- value cutoff of <1.0 e-6 against sequences from other known molluscan biomineralization genes or sequences that were highly expressed in the Crassostrea gigas mantle (Zhang et al., 2012). The Genbank accession number (Molluscan Match) is reported for each identification (BLAST).
Zhang G, Fang X, Guo X, et al. (2012) The oyster genome reveals stress adaptation and complexity of shell formation. Nature 490, 49-54
Molluscan length # min. similarity Transcript ID Match BLAST (bp) hits e-value mean GAWL01002040 ABO10190.1 67kd laminin receptor precursor 1159 1 4.50E-127 80.00% af484096_1calcium calmodulin-dependent serine protein GAWL01007520 AAO83853.1 kinase 1 352 2 1.20E-09 51.00% af484096_1calcium calmodulin-dependent serine protein GAWL01008523 AAO83853.1 kinase 1 201 1 5.20E-09 59.00% af484096_1calcium calmodulin-dependent serine protein GAWL01008525 AAO83853.1 kinase 1 233 2 3.30E-12 57.50% af484096_1calcium calmodulin-dependent serine protein GAWL01008839 AAO83853.1 kinase 1 959 1 2.30E-07 45.00% af484096_1calcium calmodulin-dependent serine protein GAWL01009550 AAO83853.1 kinase 1 313 2 4.00E-08 47.50% af484096_1calcium calmodulin-dependent serine protein GAWL01010250 AAO83853.1 kinase 1 481 1 5.00E-10 48.00% af484096_1calcium calmodulin-dependent serine protein GAWL01012098 AAO83853.1 kinase 1 688 2 1.20E-15 56.00% af484096_1calcium calmodulin-dependent serine protein GAWL01012491 AAO83853.1 kinase 1 697 2 1.90E-14 43.50% af484096_1calcium calmodulin-dependent serine protein GAWL01012519 AAO83853.1 kinase 1 1316 2 3.60E-13 46.50% af484096_1calcium calmodulin-dependent serine protein GAWL01014498 AAO83853.1 kinase 1 2426 2 1.00E-41 48.50% af484096_1calcium calmodulin-dependent serine protein GAWL01020303 AAO83853.1 kinase 1 1466 2 1.90E-17 43.00% af484096_1calcium calmodulin-dependent serine protein GAWL01022326 AAO83853.1 kinase 1 1794 1 3.20E-19 47.00% af484096_1calcium calmodulin-dependent serine protein GAWL01022800 AAO83853.1 kinase 1 1724 3 2.00E-15 48.33%
af484096_1calcium calmodulin-dependent serine protein GAWL01029840 AAO83853.1 kinase 1 2010 2 2.10E-19 43.50% af484096_1calcium calmodulin-dependent serine protein GAWL01029841 AAO83853.1 kinase 1 3422 2 5.70E-19 43.50% af484096_1calcium calmodulin-dependent serine protein GAWL01030131 AAO83853.1 kinase 1 1853 2 1.30E-13 40.50% af484096_1calcium calmodulin-dependent serine protein GAWL01031237 AAO83853.1 kinase 1 1837 2 1.30E-12 44.50% af484096_1calcium calmodulin-dependent serine protein GAWL01031239 AAO83853.1 kinase 1 1771 2 1.20E-12 44.50% af484096_1calcium calmodulin-dependent serine protein GAWL01041551 AAO83853.1 kinase 1 1537 1 1.00E-07 44.00% af484096_1calcium calmodulin-dependent serine protein GAWL01042634 AAO83853.1 kinase 1 638 2 2.20E-11 41.50% af484096_1calcium calmodulin-dependent serine protein GAWL01043425 AAO83853.1 kinase 1 341 2 7.80E-11 53.50% af484096_1calcium calmodulin-dependent serine protein GAWL01001168 AAO83853.1 kinase 1 1134 3 1.70E-16 43.00% af484096_1calcium calmodulin-dependent serine protein GAWL01001865 AAO83853.1 kinase 1 1961 2 3.80E-08 51.00% af484096_1calcium calmodulin-dependent serine protein GAWL01002231 AAO83853.1 kinase 1 1455 2 9.00E-21 44.50% af484096_1calcium calmodulin-dependent serine protein GAWL01002699 AAO83853.1 kinase 1 913 2 8.10E-12 47.50% af484096_1calcium calmodulin-dependent serine protein GAWL01004308 AAO83853.1 kinase 1 420 2 2.40E-07 48.00% GAWL01006046 AAV69062.1 alkaline phosphatase 243 1 1.20E-20 68.00% GAWL01007068 AAV69062.1 alkaline phosphatase 228 1 3.30E-11 62.00% GAWL01021034 AAV69062.1 alkaline phosphatase 955 1 4.10E-51 68.00% GAWL01018085 ABF13208.1 amorphous calcium carbonate binding protein 1 1414 1 1.30E-11 43.00% GAWL01019754 ABF13208.1 amorphous calcium carbonate binding protein 1 1051 1 1.70E-10 45.00% GAWL01021558 ABF13208.1 amorphous calcium carbonate binding protein 1 307 1 3.80E-08 47.00% GAWL01022734 ABF13208.1 amorphous calcium carbonate binding protein 1 1561 1 1.30E-08 41.00% GAWL01023481 ABF13208.1 amorphous calcium carbonate binding protein 1 1273 1 4.30E-13 40.00% GAWL01028996 ABF13208.1 amorphous calcium carbonate binding protein 1 776 1 1.70E-20 45.00%
GAWL01036492 ABF13208.1 amorphous calcium carbonate binding protein 1 1557 1 1.60E-10 37.00% GAWL01037328 ABF13208.1 amorphous calcium carbonate binding protein 1 876 1 5.80E-21 47.00% GAWL01037329 ABF13208.1 amorphous calcium carbonate binding protein 1 865 1 4.40E-21 47.00% GAWL01016209 AAQ63463.1 BMSP 617 2 4.30E-09 48.50% GAWL01005835 BAK86420.1 BMSP 1237 1 3.20E-17 48.00% GAWL01007088 BAK86420.1 BMSP 266 1 3.10E-12 60.00% GAWL01007170 BAK86420.1 BMSP 1139 1 2.60E-15 45.00% GAWL01007692 BAK86420.1 BMSP 306 1 6.40E-08 61.00% GAWL01009973 BAK86420.1 BMSP 635 1 8.20E-11 53.00% GAWL01011067 BAK86420.1 BMSP 1210 2 2.90E-33 44.50% GAWL01011767 BAK86420.1 BMSP 1392 1 4.00E-08 44.00% GAWL01012090 BAK86420.1 BMSP 350 1 7.30E-07 52.00% GAWL01016206 BAK86420.1 BMSP 1163 1 3.40E-15 43.00% GAWL01018515 BAK86420.1 BMSP 2154 2 7.30E-20 46.00% GAWL01018538 BAK86420.1 BMSP 2099 1 6.50E-29 43.00% GAWL01019168 BAK86420.1 BMSP 1780 2 1.00E-07 46.50% GAWL01019169 BAK86420.1 BMSP 1831 2 1.10E-07 46.50% GAWL01021256 BAK86420.1 BMSP 1554 1 8.60E-12 48.00% GAWL01021777 BAK86420.1 BMSP 1054 1 3.80E-11 49.00% GAWL01021778 BAK86420.1 BMSP 963 1 5.50E-07 47.00% GAWL01023192 BAK86420.1 BMSP 353 1 8.80E-09 47.00% GAWL01023193 BAK86420.1 BMSP 341 1 3.00E-11 50.00% GAWL01023911 BAK86420.1 BMSP 1342 2 2.10E-16 39.00% GAWL01024166 BAK86420.1 BMSP 912 1 5.00E-30 45.00% GAWL01024167 BAK86420.1 BMSP 840 1 1.00E-29 45.00% GAWL01024373 BAK86420.1 BMSP 836 1 2.20E-19 54.00% GAWL01024795 BAK86420.1 BMSP 1739 1 8.10E-19 54.00% GAWL01024796 BAK86420.1 BMSP 1783 1 8.60E-19 54.00% GAWL01026614 BAK86420.1 BMSP 968 1 1.80E-07 45.00%
GAWL01027077 BAK86420.1 BMSP 645 1 7.80E-09 60.00% GAWL01027487 BAK86420.1 BMSP 391 1 1.30E-09 52.00% GAWL01027568 BAK86420.1 BMSP 2341 1 2.10E-09 45.00% GAWL01027593 BAK86420.1 BMSP 953 1 3.20E-17 48.00% GAWL01027594 BAK86420.1 BMSP 902 1 2.90E-17 48.00% GAWL01027595 BAK86420.1 BMSP 977 1 3.60E-17 48.00% GAWL01027596 BAK86420.1 BMSP 744 1 1.70E-17 48.00% GAWL01028835 BAK86420.1 BMSP 1121 1 5.30E-08 41.00% GAWL01028836 BAK86420.1 BMSP 1109 1 5.30E-08 41.00% GAWL01029398 BAK86420.1 BMSP 3047 2 2.40E-71 43.00% GAWL01029399 BAK86420.1 BMSP 3003 2 2.80E-71 43.00% GAWL01029400 BAK86420.1 BMSP 549 1 8.40E-22 54.00% GAWL01029531 BAK86420.1 BMSP 1859 1 6.20E-20 43.00% GAWL01029612 BAK86420.1 BMSP 1649 1 1.40E-20 50.00% GAWL01029613 BAK86420.1 BMSP 1722 1 1.60E-20 50.00% GAWL01029935 BAK86420.1 BMSP 1935 1 1.70E-22 46.00% GAWL01030751 BAK86420.1 BMSP 2672 1 1.10E-14 50.00% GAWL01032487 BAK86420.1 BMSP 556 1 2.50E-08 59.00% GAWL01032488 BAK86420.1 BMSP 1836 1 2.00E-28 45.00% GAWL01032490 BAK86420.1 BMSP 1955 1 7.20E-22 40.00% GAWL01032730 BAK86420.1 BMSP 1341 1 2.10E-09 55.00% GAWL01040474 BAK86420.1 BMSP 1021 1 1.70E-21 49.00% GAWL01040934 BAK86420.1 BMSP 1580 2 1.70E-33 44.50% GAWL01044612 BAK86420.1 BMSP 452 1 6.30E-10 49.00% GAWL01006819 ACI96106.1 calcineurin a subunit 1242 1 1.20E-64 59.00% GAWL01002013 ACI96106.1 calcineurin a subunit 2175 1 3.50E-65 63.00% GAWL01003543 ACI96106.1 calcineurin a subunit 611 1 2.40E-82 93.00% GAWL01003544 ACI96106.1 calcineurin a subunit 339 1 3.90E-83 99.00% GAWL01011445 ACI96107.1 calcineurin b subunit 726 1 1.10E-08 47.00%
GAWL01022964 ACI96107.1 calcineurin b subunit 913 2 1.30E-09 50.50% GAWL01030364 ACI96107.1 calcineurin b subunit 1408 1 1.10E-07 52.00% GAWL01038612 ACI96107.1 calcineurin b subunit 821 1 1.20E-10 49.00% GAWL01038613 ACI96107.1 calcineurin b subunit 593 1 4.20E-11 49.00% GAWL01005846 AAU93878.1 calcium-dependent protein kinase 919 1 2.10E-26 61.00% GAWL01024944 AAU93878.1 calcium-dependent protein kinase 679 1 5.40E-08 49.00% GAWL01025644 AAU93878.1 calcium-dependent protein kinase 239 1 2.20E-07 64.00% GAWL01004991 AAU93878.1 calcium-dependent protein kinase 941 4 1.40E-17 45.50% GAWL01022742 AAQ20043.1 calmodulin 1276 2 1.60E-11 49.00% GAWL01008433 ACI22622.1 calmodulin 236 2 7.80E-07 54.00% GAWL01014244 ACI22622.1 calmodulin 231 2 8.70E-10 53.00% GAWL01014561 ACI22622.1 calmodulin 306 2 8.70E-15 94.00% GAWL01024577 ACI22622.1 calmodulin 435 2 1.90E-07 55.50% GAWL01011531 AAQ20043.1 calmodulin-like protein 5590 3 3.90E-12 54.67% GAWL01013700 AAQ20043.1 calmodulin-like protein 540 4 6.20E-32 60.25% GAWL01014562 AAQ20043.1 calmodulin-like protein 335 6 1.30E-63 71.50% GAWL01014655 AAQ20043.1 calmodulin-like protein 1710 3 1.70E-26 58.00% GAWL01015148 AAQ20043.1 calmodulin-like protein 897 3 7.00E-24 51.67% GAWL01017236 AAQ20043.1 calmodulin-like protein 1500 3 5.60E-31 61.67% GAWL01023182 AAQ20043.1 calmodulin-like protein 1017 3 5.60E-23 58.33% GAWL01023184 AAQ20043.1 calmodulin-like protein 1273 4 6.20E-24 55.75% GAWL01023185 AAQ20043.1 calmodulin-like protein 1293 4 6.80E-24 56.00% GAWL01023919 AAQ20043.1 calmodulin-like protein 1897 4 1.90E-27 68.25% GAWL01025368 AAQ20043.1 calmodulin-like protein 766 3 1.30E-22 54.00% GAWL01025369 AAQ20043.1 calmodulin-like protein 738 3 9.90E-23 54.00% GAWL01025370 AAQ20043.1 calmodulin-like protein 999 3 1.50E-22 54.00% GAWL01026783 AAQ20043.1 calmodulin-like protein 1722 3 8.00E-34 65.00% GAWL01034793 AAQ20043.1 calmodulin-like protein 830 4 3.60E-13 50.50% GAWL01034794 AAQ20043.1 calmodulin-like protein 776 4 2.70E-13 50.50%
GAWL01041627 AAQ20043.1 calmodulin-like protein 937 5 3.60E-38 61.80% GAWL01002706 AAQ20043.1 calmodulin-like protein 644 4 2.50E-12 48.75% GAWL01004554 AAQ20043.1 calmodulin-like protein 685 3 3.10E-26 62.33% GAWL01012421 AAV73912.1 calmodulin-like protein 1427 4 3.20E-09 59.00% GAWL01017805 AAV73912.1 calmodulin-like protein 2042 1 3.20E-11 55.00% GAWL01045336 AAV73912.1 calmodulin-like protein 250 3 1.60E-08 50.00% GAWL01005925 ACI22622.1 calmodulin-like protein 745 3 5.50E-26 58.33% GAWL01006019 ACI22622.1 calmodulin-like protein 293 4 1.80E-16 67.00% GAWL01007472 ACI22622.1 calmodulin-like protein 649 4 4.10E-26 62.00% GAWL01009136 ACI22622.1 calmodulin-like protein 734 4 1.80E-53 65.00% GAWL01009322 ACI22622.1 calmodulin-like protein 708 3 1.80E-08 45.67% GAWL01014441 ACI22622.1 calmodulin-like protein 633 3 4.60E-16 50.67% GAWL01018034 ACI22622.1 calmodulin-like protein 736 4 3.90E-45 66.75% GAWL01018851 ACI22622.1 calmodulin-like protein 860 5 6.00E-39 61.00% GAWL01019614 ACI22622.1 calmodulin-like protein 655 3 3.30E-12 47.67% GAWL01019615 ACI22622.1 calmodulin-like protein 772 3 5.60E-12 47.00% GAWL01019780 ACI22622.1 calmodulin-like protein 1222 3 6.70E-24 57.67% GAWL01019781 ACI22622.1 calmodulin-like protein 643 3 2.10E-26 58.67% GAWL01019782 ACI22622.1 calmodulin-like protein 1249 3 2.20E-25 58.67% GAWL01019783 ACI22622.1 calmodulin-like protein 1222 3 5.90E-24 57.67% GAWL01019784 ACI22622.1 calmodulin-like protein 616 3 6.20E-25 57.67% GAWL01019785 ACI22622.1 calmodulin-like protein 616 3 5.50E-25 57.67% GAWL01022582 ACI22622.1 calmodulin-like protein 917 3 7.30E-28 63.00% GAWL01025672 ACI22622.1 calmodulin-like protein 1063 4 3.40E-21 52.50% GAWL01025673 ACI22622.1 calmodulin-like protein 623 4 8.00E-22 52.50% GAWL01025754 ACI22622.1 calmodulin-like protein 1075 6 4.00E-100 72.33% GAWL01025755 ACI22622.1 calmodulin-like protein 968 6 1.20E-100 72.33% GAWL01025756 ACI22622.1 calmodulin-like protein 1043 6 2.80E-100 72.33% GAWL01025757 ACI22622.1 calmodulin-like protein 1112 6 8.10E-101 72.33%
GAWL01025758 ACI22622.1 calmodulin-like protein 1005 6 2.40E-101 72.33% GAWL01025759 ACI22622.1 calmodulin-like protein 1080 6 5.60E-101 72.33% GAWL01032739 ACI22622.1 calmodulin-like protein 1349 3 9.50E-27 59.67% GAWL01032740 ACI22622.1 calmodulin-like protein 2042 3 5.30E-26 59.67% GAWL01032742 ACI22622.1 calmodulin-like protein 695 3 3.50E-28 60.33% GAWL01033371 ACI22622.1 calmodulin-like protein 1319 3 2.30E-21 54.67% GAWL01033372 ACI22622.1 calmodulin-like protein 916 3 4.60E-22 54.67% GAWL01040509 ACI22622.1 calmodulin-like protein 2132 6 3.80E-98 72.17% GAWL01001403 ACI22622.1 calmodulin-like protein 245 3 1.60E-09 68.67% GAWL01040477 ABR68546.1 calreticulin 1807 1 1.40E-178 87.00% GAWL01006317 AAX16122.1 carbonic anhydrase precursor 372 8 6.60E-14 55.00% GAWL01014728 AAX16122.1 carbonic anhydrase precursor 501 8 1.10E-41 49.50% GAWL01025152 AAX16122.1 carbonic anhydrase precursor 1322 8 2.50E-50 45.75% GAWL01025154 AAX16122.1 carbonic anhydrase precursor 1370 8 6.40E-30 44.25% GAWL01030114 AAX16122.1 carbonic anhydrase precursor 571 8 1.30E-13 52.13% GAWL01030115 AAX16122.1 carbonic anhydrase precursor 1145 8 1.10E-12 52.25% GAWL01030119 AAX16122.1 carbonic anhydrase precursor 966 8 6.60E-13 52.25% GAWL01030121 AAX16122.1 carbonic anhydrase precursor 591 8 1.20E-13 52.13% GAWL01036321 AAX16122.1 carbonic anhydrase precursor 1252 8 7.10E-38 58.00% GAWL01041171 AAX16122.1 carbonic anhydrase precursor 502 2 3.50E-17 53.00% GAWL01043375 AAX16122.1 carbonic anhydrase precursor 267 2 2.60E-11 56.00% GAWL01011206 BAJ52887.1 carbonic anhydrase-related protein viii 845 8 3.60E-53 48.50% GAWL01030123 BAJ52887.1 carbonic anhydrase-related protein viii 715 8 8.90E-14 53.13% GAWL01021323 AAY86556.1 chitin synthase 6849 2 2.70E-17 43.50% GAWL01040313 AAY86556.1 chitin synthase 939 2 7.30E-47 61.00% GAWL01040314 AAY86556.1 chitin synthase 638 2 8.10E-39 56.00% GAWL01040294 AAY86556.1 chitin synthase 1047 2 4.20E-43 47.50% GAWL01040301 BAF73720.1 chitin synthase 2448 2 1.30E-50 44.50% GAWL01040302 BAF73720.1 chitin synthase 2786 2 3.30E-50 44.50%
GAWL01040310 BAF73720.1 chitin synthase 1561 2 9.40E-47 47.00% GAWL01040311 BAF73720.1 chitin synthase 2377 2 3.90E-45 47.00% GAWL01040312 BAF73720.1 chitin synthase 1755 2 3.80E-53 43.50% GAWL01040318 BAF73720.1 chitin synthase 2039 2 1.60E-45 47.00% GAWL01006661 ACO36045.1 c-type lectin 1 770 1 7.90E-07 52.00% GAWL01006662 ACO36045.1 c-type lectin 1 730 1 7.10E-07 52.00% GAWL01008024 ACO36045.1 c-type lectin 1 1032 2 6.60E-12 43.00% GAWL01008710 ACO36045.1 c-type lectin 1 432 1 9.20E-11 55.00% GAWL01015091 ACO36045.1 c-type lectin 1 1157 6 3.80E-14 44.17% GAWL01015921 ACO36045.1 c-type lectin 1 276 2 1.20E-09 51.00% GAWL01021731 ACO36045.1 c-type lectin 1 2114 1 1.20E-08 39.00% GAWL01023945 ACO36045.1 c-type lectin 1 210 1 3.70E-14 60.00% GAWL01024711 ACO36045.1 c-type lectin 1 1360 1 9.20E-08 46.00% GAWL01026387 ACO36045.1 c-type lectin 1 3322 3 6.20E-13 43.67% GAWL01030133 ACO36045.1 c-type lectin 1 300 2 1.20E-07 46.50% GAWL01033405 ACO36045.1 c-type lectin 1 545 6 1.10E-11 49.67% GAWL01033407 ACO36045.1 c-type lectin 1 580 6 2.10E-11 49.67% GAWL01038897 ACO36045.1 c-type lectin 1 1192 1 7.60E-07 48.00% GAWL01038905 ACO36045.1 c-type lectin 1 643 1 4.00E-07 48.00% GAWL01039996 ACO36045.1 c-type lectin 1 999 1 3.70E-08 53.00% GAWL01040010 ACO36045.1 c-type lectin 1 287 2 6.30E-09 49.00% GAWL01040002 ACO36045.1 c-type lectin 1 887 1 3.00E-08 53.00% GAWL01040664 ACO36045.1 c-type lectin 1 662 1 5.40E-16 49.00% GAWL01041666 ACO36045.1 c-type lectin 1 531 1 6.10E-09 49.00% GAWL01043724 ACO36045.1 c-type lectin 1 563 2 2.20E-12 47.50% GAWL01044548 ACO36045.1 c-type lectin 1 542 1 1.80E-09 41.00% GAWL01023942 ADD16957.1 c-type lectin 1 736 5 4.20E-15 43.20% GAWL01006345 ACO36046.1 c-type lectin 2 243 3 6.20E-10 51.33% GAWL01008852 ACO36046.1 c-type lectin 2 492 3 5.00E-10 45.33%
GAWL01011796 ACO36046.1 c-type lectin 2 324 2 4.90E-07 50.50% GAWL01013902 ACO36046.1 c-type lectin 2 475 2 1.60E-12 45.00% GAWL01016430 ACO36046.1 c-type lectin 2 630 1 3.90E-08 38.00% GAWL01018912 ACO36046.1 c-type lectin 2 398 2 1.20E-09 50.50% GAWL01018913 ACO36046.1 c-type lectin 2 439 2 1.10E-09 49.00% GAWL01023929 ACO36046.1 c-type lectin 2 1029 2 3.90E-10 44.50% GAWL01028413 ACO36046.1 c-type lectin 2 896 8 2.00E-16 49.38% GAWL01028415 ACO36046.1 c-type lectin 2 860 8 1.80E-16 49.38% GAWL01030072 ACO36046.1 c-type lectin 2 947 2 1.60E-08 41.50% GAWL01034777 ACO36046.1 c-type lectin 2 432 2 2.90E-08 48.00% GAWL01037099 ACO36046.1 c-type lectin 2 556 1 2.90E-08 42.00% GAWL01044073 ACO36046.1 c-type lectin 2 251 1 4.80E-07 47.00% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01005427 ABO26644.1 rich acidic matrix protein short=tramp 647 2 9.70E-22 50.00% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01006549 ABO26644.1 rich acidic matrix protein short=tramp 751 2 5.00E-25 48.50% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01007316 ABO26644.1 rich acidic matrix protein short=tramp 396 2 8.20E-09 55.50% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01011602 ABO26644.1 rich acidic matrix protein short=tramp 748 2 6.60E-13 46.50% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01012778 ABO26644.1 rich acidic matrix protein short=tramp 559 2 1.70E-11 56.50% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01013075 ABO26644.1 rich acidic matrix protein short=tramp 648 2 3.30E-34 52.50% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01016999 ABO26644.1 rich acidic matrix protein short=tramp 678 2 5.00E-34 51.50% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01018852 ABO26644.1 rich acidic matrix protein short=tramp 775 2 3.40E-11 58.00% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01018853 ABO26644.1 rich acidic matrix protein short=tramp 826 2 4.10E-11 58.00% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01020398 ABO26644.1 rich acidic matrix protein short=tramp 676 2 5.30E-24 46.50% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01022052 ABO26644.1 rich acidic matrix protein short=tramp 657 2 2.70E-29 49.50%
derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01026459 ABO26644.1 rich acidic matrix protein short=tramp 1333 2 8.80E-20 56.50% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01033187 ABO26644.1 rich acidic matrix protein short=tramp 452 2 8.70E-24 58.50% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01033189 ABO26644.1 rich acidic matrix protein short=tramp 458 2 9.70E-24 58.50% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01033190 ABO26644.1 rich acidic matrix protein short=tramp 1279 2 9.50E-20 61.00% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01033191 ABO26644.1 rich acidic matrix protein short=tramp 1242 2 8.50E-20 61.00% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01040568 ABO26644.1 rich acidic matrix protein short=tramp 561 2 9.70E-12 51.00% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01001212 ABO26644.1 rich acidic matrix protein short=tramp 452 2 4.00E-31 53.50% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01002418 ABO26644.1 rich acidic matrix protein short=tramp 689 2 3.60E-22 48.00% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01009495 P83553.1 rich acidic matrix protein short=tramp 357 2 1.30E-18 57.50% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01016131 P83553.1 rich acidic matrix protein short=tramp 366 2 1.40E-16 55.50% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01017752 P83553.1 rich acidic matrix protein short=tramp 783 1 7.60E-07 53.00% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01017753 P83553.1 rich acidic matrix protein short=tramp 771 1 7.50E-07 53.00% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01019808 P83553.1 rich acidic matrix protein short=tramp 757 2 1.60E-33 53.50% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01021600 P83553.1 rich acidic matrix protein short=tramp 694 1 1.50E-08 60.00% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01024781 P83553.1 rich acidic matrix protein short=tramp 1110 1 4.90E-08 55.00% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01024782 P83553.1 rich acidic matrix protein short=tramp 1187 1 5.30E-08 55.00% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01034338 P83553.1 rich acidic matrix protein short=tramp 730 2 3.90E-32 52.50% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01041264 P83553.1 rich acidic matrix protein short=tramp 339 1 9.30E-10 63.00% derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01001213 P83553.1 rich acidic matrix protein short=tramp 302 2 2.60E-09 58.50%
derm_biogl ame: full=dermatopontin ame: full=tyrosine- GAWL01001346 P83553.1 rich acidic matrix protein short=tramp 277 2 1.00E-09 57.50% GAWL01026575 ABO26644.1 dermatopontin 2342 1 2.90E-08 67.00% GAWL01026577 ABO26644.1 dermatopontin 2449 1 3.10E-08 67.00% GAWL01029650 ABO26644.1 dermatopontin 789 1 2.50E-08 62.00% GAWL01029651 ABO26644.1 dermatopontin 2740 1 1.20E-07 62.00% GAWL01033188 ABO26644.1 dermatopontin 397 1 8.80E-08 58.00% GAWL01005477 AAQ63463.1 ep protein precursor 768 1 2.80E-13 55.00% GAWL01006118 AAQ63463.1 ep protein precursor 456 1 1.00E-10 56.00% GAWL01006208 AAQ63463.1 ep protein precursor 561 1 2.70E-11 47.00% GAWL01007529 AAQ63463.1 ep protein precursor 991 1 6.80E-09 45.00% GAWL01008640 AAQ63463.1 ep protein precursor 329 1 6.80E-08 50.00% GAWL01009297 AAQ63463.1 ep protein precursor 399 1 2.20E-07 47.00% GAWL01009403 AAQ63463.1 ep protein precursor 656 2 4.30E-16 45.50% GAWL01010205 AAQ63463.1 ep protein precursor 448 2 2.10E-08 48.00% GAWL01014391 AAQ63463.1 ep protein precursor 1021 1 3.80E-08 51.00% GAWL01014590 AAQ63463.1 ep protein precursor 703 1 2.50E-08 42.00% GAWL01015316 AAQ63463.1 ep protein precursor 421 1 6.10E-10 52.00% GAWL01015660 AAQ63463.1 ep protein precursor 411 2 2.90E-13 50.00% GAWL01015661 AAQ63463.1 ep protein precursor 395 2 3.10E-10 46.00% GAWL01016805 AAQ63463.1 ep protein precursor 535 2 2.30E-10 48.00% GAWL01016806 AAQ63463.1 ep protein precursor 522 2 1.10E-10 47.50% GAWL01017973 AAQ63463.1 ep protein precursor 1128 1 5.00E-08 45.00% GAWL01019476 AAQ63463.1 ep protein precursor 523 2 3.50E-10 48.50% GAWL01019625 AAQ63463.1 ep protein precursor 407 1 1.40E-10 46.00% GAWL01020125 AAQ63463.1 ep protein precursor 626 2 2.60E-08 47.00% GAWL01020345 AAQ63463.1 ep protein precursor 977 1 4.10E-08 45.00% GAWL01020877 AAQ63463.1 ep protein precursor 692 2 1.20E-07 47.00% GAWL01021530 AAQ63463.1 ep protein precursor 609 2 1.30E-12 47.00% GAWL01021543 AAQ63463.1 ep protein precursor 502 1 3.00E-07 48.00%
GAWL01021690 AAQ63463.1 ep protein precursor 531 2 5.40E-14 54.00% GAWL01021993 AAQ63463.1 ep protein precursor 432 2 5.50E-11 46.50% GAWL01021994 AAQ63463.1 ep protein precursor 448 2 7.90E-12 46.50% GAWL01022422 AAQ63463.1 ep protein precursor 1146 2 2.40E-15 49.00% GAWL01022457 AAQ63463.1 ep protein precursor 1167 2 7.00E-14 47.50% GAWL01022513 AAQ63463.1 ep protein precursor 682 1 1.70E-10 41.00% GAWL01022518 AAQ63463.1 ep protein precursor 800 1 5.60E-09 47.00% GAWL01022649 AAQ63463.1 ep protein precursor 618 1 2.20E-10 53.00% GAWL01022650 AAQ63463.1 ep protein precursor 660 1 2.80E-10 53.00% GAWL01025037 AAQ63463.1 ep protein precursor 645 2 6.00E-08 42.50% GAWL01025638 AAQ63463.1 ep protein precursor 422 2 6.40E-12 51.50% GAWL01025630 AAQ63463.1 ep protein precursor 830 2 5.30E-16 44.50% GAWL01025631 AAQ63463.1 ep protein precursor 645 2 1.20E-16 46.00% GAWL01025635 AAQ63463.1 ep protein precursor 607 2 1.70E-11 49.50% GAWL01025637 AAQ63463.1 ep protein precursor 422 1 1.10E-11 49.00% GAWL01025714 AAQ63463.1 ep protein precursor 763 1 1.40E-09 46.00% GAWL01025721 AAQ63463.1 ep protein precursor 857 1 1.80E-09 46.00% GAWL01030734 AAQ63463.1 ep protein precursor 785 2 6.80E-10 45.00% GAWL01030725 AAQ63463.1 ep protein precursor 849 2 2.50E-14 45.50% GAWL01031677 AAQ63463.1 ep protein precursor 561 2 5.60E-10 53.00% GAWL01031678 AAQ63463.1 ep protein precursor 1331 2 5.30E-09 53.00% GAWL01032127 AAQ63463.1 ep protein precursor 402 1 3.50E-07 55.00% GAWL01034030 AAQ63463.1 ep protein precursor 1064 2 1.10E-09 48.00% GAWL01034031 AAQ63463.1 ep protein precursor 860 2 3.20E-09 47.00% GAWL01035489 AAQ63463.1 ep protein precursor 1185 2 1.20E-14 44.00% GAWL01035492 AAQ63463.1 ep protein precursor 1186 2 1.50E-14 48.00% GAWL01035495 AAQ63463.1 ep protein precursor 1461 2 6.70E-10 45.50% GAWL01035484 AAQ63463.1 ep protein precursor 1206 2 5.80E-14 48.00% GAWL01035486 AAQ63463.1 ep protein precursor 1368 2 7.40E-14 47.50%
GAWL01038309 AAQ63463.1 ep protein precursor 488 2 7.10E-11 49.50% GAWL01040758 AAQ63463.1 ep protein precursor 850 2 9.90E-15 47.50% GAWL01040964 AAQ63463.1 ep protein precursor 662 2 5.50E-16 47.00% GAWL01041273 AAQ63463.1 ep protein precursor 671 1 5.20E-13 44.00% GAWL01041785 AAQ63463.1 ep protein precursor 371 2 2.30E-09 46.50% GAWL01000795 AAQ63463.1 ep protein precursor 400 1 4.80E-08 49.00% GAWL01000818 AAQ63463.1 ep protein precursor 361 2 6.30E-09 52.00% GAWL01002287 AAQ63463.1 ep protein precursor 503 2 3.40E-12 47.50% GAWL01002564 AAQ63463.1 ep protein precursor 765 1 1.30E-12 46.00% GAWL01002565 AAQ63463.1 ep protein precursor 483 1 2.40E-07 55.00% GAWL01003925 AAQ63463.1 ep protein precursor 468 2 6.50E-13 49.00% GAWL01000494 BAK86420.1 ep protein precursor 256 1 2.80E-08 48.00% epdr1_halai ame: full=ependymin-related protein 1 flags: GAWL01017133 P86734.1 precursor 972 1 2.50E-08 42.00% epdr1_halai ame: full=ependymin-related protein 1 flags: GAWL01017134 P86734.1 precursor 1035 1 2.70E-08 42.00% GAWL01016318 AAQ12076.1 ferritin-like protein 367 1 1.00E-11 64.00% GAWL01022407 AAQ12076.1 ferritin-like protein 1108 1 4.20E-101 91.00% GAWL01037867 AAQ12076.1 ferritin-like protein 902 1 2.20E-68 70.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01005727 Q5GIS3.1 beta-1 470 1 8.90E-63 98.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01005888 Q5GIS3.1 beta-1 775 1 8.30E-11 40.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01008644 Q5GIS3.1 beta-1 442 1 1.40E-07 42.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01008645 Q5GIS3.1 beta-1 312 1 2.10E-10 45.00% gbb_pinfu ame: full=guanine nucleotide-binding protein GAWL01008849 Q5GIS3.1 subunit beta short=pfgbeta1 ame: full=g protein subunit 1322 1 9.70E-22 45.00%
beta-1 gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01011746 Q5GIS3.1 beta-1 1168 1 5.20E-10 37.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01011768 Q5GIS3.1 beta-1 354 1 5.80E-07 40.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01011945 Q5GIS3.1 beta-1 1619 1 8.60E-15 44.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01012560 Q5GIS3.1 beta-1 2248 1 6.50E-09 38.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01013576 Q5GIS3.1 beta-1 780 1 1.70E-07 44.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01000477 Q5GIS3.1 beta-1 441 1 5.70E-17 50.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01015422 Q5GIS3.1 beta-1 2546 1 4.00E-10 40.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01015738 Q5GIS3.1 beta-1 1077 1 6.00E-09 41.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01017139 Q5GIS3.1 beta-1 260 1 5.60E-08 47.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01017328 Q5GIS3.1 beta-1 1635 1 3.80E-07 45.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01019381 Q5GIS3.1 beta-1 1808 1 1.20E-10 59.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01019979 Q5GIS3.1 beta-1 1368 1 5.10E-11 40.00%
gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01021552 Q5GIS3.1 beta-1 879 1 2.50E-07 39.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01022312 Q5GIS3.1 beta-1 2005 1 1.40E-13 42.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01022313 Q5GIS3.1 beta-1 374 1 2.30E-07 43.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01022625 Q5GIS3.1 beta-1 1266 1 2.00E-11 43.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01022647 Q5GIS3.1 beta-1 1539 1 5.30E-10 41.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01024035 Q5GIS3.1 beta-1 1803 1 1.70E-18 46.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01024253 Q5GIS3.1 beta-1 1761 1 2.30E-14 38.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01024950 Q5GIS3.1 beta-1 1677 1 5.40E-16 43.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01025255 Q5GIS3.1 beta-1 1376 1 3.70E-12 40.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01027665 Q5GIS3.1 beta-1 1774 1 5.40E-14 38.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01027666 Q5GIS3.1 beta-1 573 1 3.30E-14 46.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01031818 Q5GIS3.1 beta-1 2638 1 6.10E-23 40.00% gbb_pinfu ame: full=guanine nucleotide-binding protein GAWL01031982 Q5GIS3.1 subunit beta short=pfgbeta1 ame: full=g protein subunit 1831 1 6.80E-09 39.00%
beta-1 gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01034877 Q5GIS3.1 beta-1 1860 1 7.50E-17 42.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01034878 Q5GIS3.1 beta-1 1847 1 7.40E-17 42.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01040454 Q5GIS3.1 beta-1 1151 1 5.10E-17 43.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01040512 Q5GIS3.1 beta-1 1155 1 2.80E-11 37.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01040575 Q5GIS3.1 beta-1 1124 1 2.40E-12 39.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01040609 Q5GIS3.1 beta-1 1700 1 7.10E-22 43.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01043478 Q5GIS3.1 beta-1 375 1 9.20E-09 43.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01045568 Q5GIS3.1 beta-1 227 1 2.00E-09 45.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01002243 Q5GIS3.1 beta-1 1113 1 6.20E-26 44.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01002292 Q5GIS3.1 beta-1 963 1 3.50E-07 40.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01003348 Q5GIS3.1 beta-1 412 1 8.50E-10 44.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01003613 Q5GIS3.1 beta-1 314 1 4.00E-07 44.00%
gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01003614 Q5GIS3.1 beta-1 981 1 4.40E-18 44.00% gbb_pinfu ame: full=guanine nucleotide-binding protein subunit beta short=pfgbeta1 ame: full=g protein subunit GAWL01003638 Q5GIS3.1 beta-1 945 1 1.20E-14 41.00% GAWL01022590 P86785.1 giga2_cragi ame: full=gigasin-2 flags: precursor 2745 1 2.30E-16 42.00% GAWL01043997 P86789.1 giga6_cragi ame: full=gigasin-6 flags: precursor 226 1 8.50E-16 62.00% GAWL01045653 P86789.1 giga6_cragi ame: full=gigasin-6 flags: precursor 256 1 7.40E-08 55.00% GAWL01032793 BAG50305.1 hypothetical protein 1694 1 2.70E-07 64.00% GAWL01032794 BAG50305.1 hypothetical protein 1563 1 2.40E-07 64.00% GAWL01023321 ADD16957.1 incilarin a 384 2 2.60E-08 43.00% GAWL01023322 ADD16957.1 incilarin a 542 7 3.40E-17 43.43% GAWL01009822 BAA19861.1 incilarin a 788 1 5.20E-08 43.00% GAWL01010316 BAA19861.1 incilarin a 214 2 3.10E-07 47.50% GAWL01013965 BAA19861.1 incilarin a 691 7 1.50E-17 44.29% GAWL01015116 BAA19861.1 incilarin a 251 1 2.40E-07 50.00% GAWL01019362 BAA19861.1 incilarin a 605 1 4.00E-08 44.00% GAWL01020137 BAA19861.1 incilarin a 881 1 2.60E-07 39.00% GAWL01021400 BAA19861.1 incilarin a 1054 1 9.20E-09 47.00% GAWL01000570 BAA19861.1 incilarin a 215 1 4.40E-07 57.00% GAWL01022500 BAA19861.1 incilarin a 651 7 8.60E-15 43.57% GAWL01022939 BAA19861.1 incilarin a 2077 1 4.80E-08 41.00% GAWL01024660 BAA19861.1 incilarin a 205 1 1.60E-09 53.00% GAWL01025751 BAA19861.1 incilarin a 550 7 8.00E-19 44.14% GAWL01027130 BAA19861.1 incilarin a 567 3 1.10E-09 46.33% GAWL01027488 BAA19861.1 incilarin a 719 1 2.00E-10 48.00% GAWL01027597 BAA19861.1 incilarin a 850 6 7.10E-17 43.00% GAWL01027598 BAA19861.1 incilarin a 856 5 4.00E-17 44.20% GAWL01027599 BAA19861.1 incilarin a 865 6 7.00E-17 43.00% GAWL01027600 BAA19861.1 incilarin a 850 4 1.40E-16 43.00%
GAWL01027601 BAA19861.1 incilarin a 865 4 1.60E-16 43.00% GAWL01027602 BAA19861.1 incilarin a 856 5 8.50E-17 44.00% GAWL01027603 BAA19861.1 incilarin a 841 5 7.00E-17 44.00% GAWL01027604 BAA19861.1 incilarin a 841 5 3.80E-17 44.20% GAWL01033359 BAA19861.1 incilarin a 2123 4 6.20E-11 41.50% GAWL01033361 BAA19861.1 incilarin a 2762 4 1.10E-10 41.00% GAWL01033393 BAA19861.1 incilarin a 222 1 5.50E-07 56.00% GAWL01040977 BAA19861.1 incilarin a 426 2 4.50E-10 49.00% GAWL01042437 BAA19861.1 incilarin a 412 1 8.50E-08 44.00% GAWL01001600 BAA19861.1 incilarin a 531 1 1.70E-09 47.00% GAWL01004462 BAA19861.1 incilarin a 452 7 1.60E-19 44.57% GAWL01004789 BAA19861.1 incilarin a 500 1 2.70E-09 49.00% GAWL01016809 BAK86420.1 incilarin a 1117 4 2.50E-21 47.25% GAWL01017803 BAK86420.1 incilarin a 2181 3 7.10E-25 44.67% GAWL01009362 BAA19863.1 incilarin c 553 5 1.30E-08 47.20% GAWL01022247 BAA19863.1 incilarin c 697 6 3.30E-19 44.33% GAWL01022248 BAA19863.1 incilarin c 668 6 3.10E-19 44.83% GAWL01024694 BAA19863.1 incilarin c 377 1 2.80E-07 42.00% GAWL01025106 BAA19863.1 incilarin c 635 4 4.90E-12 40.75% GAWL01025108 BAA19863.1 incilarin c 699 2 1.10E-10 53.00% GAWL01003112 BAA19863.1 incilarin c 424 1 4.50E-08 50.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01005591 Q4KTY1.1 homolog 1286 2 5.90E-43 52.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01008524 Q4KTY1.1 homolog 328 2 7.30E-24 56.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01011705 Q4KTY1.1 homolog 2192 2 2.40E-33 50.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01011706 Q4KTY1.1 homolog 2176 2 2.30E-33 50.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01012100 Q4KTY1.1 homolog 309 2 7.10E-28 60.00%
kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01012976 Q4KTY1.1 homolog 290 1 6.90E-07 65.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01012977 Q4KTY1.1 homolog 1312 2 5.20E-27 56.50% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01013823 Q4KTY1.1 homolog 1071 2 2.10E-10 45.50% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01015085 Q4KTY1.1 homolog 946 2 1.00E-33 52.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01017946 Q4KTY1.1 homolog 793 2 3.50E-28 47.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01018390 Q4KTY1.1 homolog 2337 2 8.20E-22 48.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01019103 Q4KTY1.1 homolog 1434 2 4.50E-23 40.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01019277 Q4KTY1.1 homolog 1811 2 6.60E-20 41.50% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01019446 Q4KTY1.1 homolog 2890 2 1.50E-15 43.50% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01019593 Q4KTY1.1 homolog 1114 3 2.00E-13 47.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01024755 Q4KTY1.1 homolog 3215 2 1.60E-16 49.50% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01024893 Q4KTY1.1 homolog 1469 2 2.90E-19 41.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01025488 Q4KTY1.1 homolog 1618 2 8.70E-21 43.50% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01025489 Q4KTY1.1 homolog 1306 2 4.50E-19 43.50% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01025490 Q4KTY1.1 homolog 1602 2 8.80E-21 42.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01025491 Q4KTY1.1 homolog 1322 2 6.70E-19 43.50% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01025493 Q4KTY1.1 homolog 1293 2 1.40E-18 44.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01025495 Q4KTY1.1 homolog 1277 2 9.00E-19 44.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01026117 Q4KTY1.1 homolog 2891 2 2.40E-66 58.50%
kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01026772 Q4KTY1.1 homolog 1496 2 7.40E-22 44.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01026773 Q4KTY1.1 homolog 1360 2 5.50E-22 44.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01026774 Q4KTY1.1 homolog 1514 2 7.60E-22 44.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01026775 Q4KTY1.1 homolog 1342 2 5.30E-22 44.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01026909 Q4KTY1.1 homolog 1532 2 1.30E-20 42.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01028721 Q4KTY1.1 homolog 2709 3 6.90E-42 47.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01028743 Q4KTY1.1 homolog 2708 2 1.10E-33 50.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01029378 Q4KTY1.1 homolog 1220 2 1.10E-21 43.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01032063 Q4KTY1.1 homolog 1418 2 3.40E-21 44.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01036701 Q4KTY1.1 homolog 2106 1 2.00E-10 45.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01036743 Q4KTY1.1 homolog 2083 2 6.80E-69 55.00% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01038312 Q4KTY1.1 homolog 3048 2 4.60E-17 43.50% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01038313 Q4KTY1.1 homolog 2809 2 4.10E-17 43.50% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01038314 Q4KTY1.1 homolog 1375 2 1.40E-15 43.50% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01038315 Q4KTY1.1 homolog 2850 2 4.20E-17 43.50% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01038316 Q4KTY1.1 homolog 3082 2 4.70E-17 43.50% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01038302 Q4KTY1.1 homolog 2816 2 4.20E-17 43.50% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01038305 Q4KTY1.1 homolog 1368 2 1.40E-15 43.50% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01038306 Q4KTY1.1 homolog 1607 2 1.90E-15 43.50%
kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01038307 Q4KTY1.1 homolog 2843 2 4.20E-17 43.50% kpsh1_pinfu ame: full=serine threonine-protein kinase h1 GAWL01040641 Q4KTY1.1 homolog 1185 3 2.70E-16 43.33% GAWL01014846 BAB03232.1 lectin 281 1 1.10E-07 45.00% GAWL01026700 BAB03232.1 lectin 1066 1 2.10E-10 40.00% GAWL01026702 BAB03232.1 lectin 930 1 2.30E-10 40.00% GAWL01032172 BAB03232.1 lectin 855 1 7.40E-07 37.00% GAWL01038774 BAB03232.1 lectin 419 1 2.40E-07 38.00% GAWL01005365 AAZ76256.1 mantle gene 2 3103 1 6.10E-30 56.00% GAWL01024519 AAZ76256.1 mantle gene 2 2012 1 1.30E-43 60.00% GAWL01024528 AAZ76256.1 mantle gene 2 1917 1 8.30E-44 60.00% GAWL01024520 AAZ76256.1 mantle gene 2 1969 1 9.50E-44 60.00% GAWL01024521 AAZ76256.1 mantle gene 2 2025 1 1.40E-43 60.00% GAWL01024523 AAZ76256.1 mantle gene 2 1930 1 8.50E-44 60.00% GAWL01024524 AAZ76256.1 mantle gene 2 1973 1 1.20E-43 60.00% GAWL01024526 AAZ76256.1 mantle gene 2 1986 1 1.20E-43 60.00% GAWL01024527 AAZ76256.1 mantle gene 2 1956 1 9.20E-44 60.00% GAWL01024644 AAZ76256.1 mantle gene 2 1413 1 2.10E-30 57.00% GAWL01000721 AAZ76256.1 mantle gene 2 842 1 1.70E-33 53.00% GAWL01013527 AAZ76258.1 mantle gene 4 296 2 6.10E-10 50.50% GAWL01016048 AAZ76258.1 mantle gene 4 447 1 1.70E-11 52.00% GAWL01016885 AAZ76258.1 mantle gene 4 398 1 2.90E-08 42.00% GAWL01018299 AAZ76258.1 mantle gene 4 591 1 6.10E-13 46.00% GAWL01019616 AAZ76258.1 mantle gene 4 663 2 4.90E-12 48.50% GAWL01019617 AAZ76258.1 mantle gene 4 766 2 5.50E-11 48.00% GAWL01020124 AAZ76258.1 mantle gene 4 753 2 1.80E-08 47.00% GAWL01020878 AAZ76258.1 mantle gene 4 682 2 6.80E-10 45.00% GAWL01020879 AAZ76258.1 mantle gene 4 682 2 5.60E-09 47.00% GAWL01020880 AAZ76258.1 mantle gene 4 692 2 1.60E-08 45.00%
GAWL01023259 AAZ76258.1 mantle gene 4 726 2 2.00E-09 43.00% GAWL01024194 AAZ76258.1 mantle gene 4 1428 1 1.20E-12 50.00% GAWL01024195 AAZ76258.1 mantle gene 4 904 1 4.20E-13 50.00% GAWL01024196 AAZ76258.1 mantle gene 4 863 1 3.60E-13 50.00% GAWL01025730 AAZ76258.1 mantle gene 4 1128 2 2.20E-11 47.50% GAWL01025704 AAZ76258.1 mantle gene 4 666 3 1.00E-09 53.67% GAWL01025735 AAZ76258.1 mantle gene 4 802 3 1.80E-11 51.67% GAWL01025705 AAZ76258.1 mantle gene 4 896 3 2.40E-11 51.67% GAWL01025706 AAZ76258.1 mantle gene 4 1140 2 2.30E-11 47.50% GAWL01029052 AAZ76258.1 mantle gene 4 554 2 5.30E-15 53.00% GAWL01029053 AAZ76258.1 mantle gene 4 690 2 6.60E-15 53.00% GAWL01029201 AAZ76258.1 mantle gene 4 870 1 3.30E-10 44.00% GAWL01038311 AAZ76258.1 mantle gene 4 675 2 4.20E-15 47.00% GAWL01038303 AAZ76258.1 mantle gene 4 1021 2 1.10E-15 51.00% GAWL01043139 AAZ76258.1 mantle gene 4 320 1 4.30E-11 48.00% GAWL01045393 AAZ76258.1 mantle gene 4 225 1 9.40E-07 54.00% GAWL01000774 AAZ76258.1 mantle gene 4 573 2 1.30E-12 48.50% GAWL01001165 AAZ76258.1 mantle gene 4 621 2 2.10E-13 47.50% GAWL01002324 AAZ76258.1 mantle gene 4 639 2 5.30E-14 50.00% GAWL01004039 AAZ76258.1 mantle gene 4 577 2 3.90E-13 46.50% GAWL01018251 AAZ22321.1 mantle protein 12 938 1 2.70E-07 61.00% GAWL01023722 AAZ22321.1 mantle protein 12 1292 1 7.40E-08 71.00% GAWL01023723 AAZ22321.1 mantle protein 12 1306 1 7.50E-08 71.00% GAWL01023724 AAZ22321.1 mantle protein 12 1179 1 6.50E-08 71.00% GAWL01023725 AAZ22321.1 mantle protein 12 1144 1 6.30E-08 71.00% GAWL01023728 AAZ22321.1 mantle protein 12 273 1 1.20E-07 56.00% GAWL01026543 AAZ22321.1 mantle protein 12 689 1 3.30E-09 61.00% GAWL01036679 AAZ22321.1 mantle protein 12 511 1 5.50E-08 52.00% GAWL01036680 AAZ22321.1 mantle protein 12 551 1 6.90E-08 52.00%
GAWL01042619 AAZ22321.1 mantle protein 12 281 1 2.70E-07 55.00% GAWL01019096 AAZ22318.1 mantle protein 9 616 1 7.50E-12 39.00% GAWL01020454 AAZ22318.1 mantle protein 9 497 1 2.40E-09 43.00% GAWL01020747 AAZ22318.1 mantle protein 9 612 1 8.20E-08 44.00% GAWL01023863 AAZ22318.1 mantle protein 9 763 1 2.10E-09 43.00% GAWL01010347 AAZ66779.2 neuronal calcium sensor-1 790 5 1.40E-32 52.40% GAWL01020837 AAZ66779.2 neuronal calcium sensor-1 1118 5 1.20E-33 52.40% GAWL01020838 AAZ66779.2 neuronal calcium sensor-1 960 5 5.80E-34 52.40% GAWL01020839 AAZ66779.2 neuronal calcium sensor-1 1185 5 1.60E-39 52.60% GAWL01022901 AAZ66779.2 neuronal calcium sensor-1 754 1 1.90E-26 51.00% GAWL01025827 AAZ66779.2 neuronal calcium sensor-1 1576 2 2.90E-32 55.00% GAWL01025828 AAZ66779.2 neuronal calcium sensor-1 1043 2 4.60E-23 56.00% GAWL01027469 AAZ66779.2 neuronal calcium sensor-1 1069 4 9.40E-16 46.75% GAWL01027470 AAZ66779.2 neuronal calcium sensor-1 1195 3 2.80E-22 49.33% GAWL01027471 AAZ66779.2 neuronal calcium sensor-1 1207 1 5.60E-10 46.00% GAWL01033972 AAZ66779.2 neuronal calcium sensor-1 961 3 1.90E-34 51.67% GAWL01044474 AAZ66779.2 neuronal calcium sensor-1 290 1 3.80E-12 53.00% GAWL01009425 ADD16957.1 perlucin 413 1 6.10E-08 43.00% GAWL01019723 ADD16957.1 perlucin 873 1 2.80E-08 53.00% GAWL01032648 ADD16957.1 perlucin 258 1 3.10E-08 50.00% GAWL01037379 ADD16957.1 perlucin 277 1 5.10E-07 46.00% GAWL01038894 ADD16957.1 perlucin 1664 1 8.70E-08 44.00% GAWL01038903 ADD16957.1 perlucin 1641 1 1.00E-07 45.00% GAWL01038886 ADD16957.1 perlucin 2213 1 9.90E-08 44.00% GAWL01038909 ADD16957.1 perlucin 2190 1 1.30E-07 45.00% GAWL01006699 ABO26596.1 perlucin 7 1135 6 8.80E-12 43.00% GAWL01007441 ABO26596.1 perlucin 7 370 2 1.50E-08 45.00% GAWL01007554 ABO26596.1 perlucin 7 494 1 6.50E-07 53.00% GAWL01010244 ABO26596.1 perlucin 7 225 1 6.70E-07 50.00%
GAWL01012361 ABO26596.1 perlucin 7 820 1 3.20E-07 43.00% GAWL01013456 ABO26596.1 perlucin 7 276 4 1.20E-12 51.50% GAWL01016387 ABO26596.1 perlucin 7 796 6 3.60E-14 43.17% GAWL01016388 ABO26596.1 perlucin 7 781 6 3.30E-14 43.17% GAWL01017017 ABO26596.1 perlucin 7 649 7 6.60E-15 44.29% GAWL01018152 ABO26596.1 perlucin 7 990 4 8.50E-13 41.50% GAWL01022940 ABO26596.1 perlucin 7 1280 1 5.50E-07 44.00% GAWL01023222 ABO26596.1 perlucin 7 1861 2 2.60E-07 42.50% GAWL01023524 ABO26596.1 perlucin 7 1785 3 2.00E-14 45.00% GAWL01023526 ABO26596.1 perlucin 7 1760 3 1.60E-14 45.00% GAWL01023865 ABO26596.1 perlucin 7 613 3 1.80E-09 44.33% GAWL01024432 ABO26596.1 perlucin 7 1014 2 2.70E-13 46.50% GAWL01024434 ABO26596.1 perlucin 7 1147 2 3.30E-13 46.50% GAWL01026679 ABO26596.1 perlucin 7 1058 1 7.10E-11 41.00% GAWL01026680 ABO26596.1 perlucin 7 1018 1 1.40E-12 41.00% GAWL01027728 ABO26596.1 perlucin 7 1780 3 1.50E-08 41.67% GAWL01027729 ABO26596.1 perlucin 7 1149 3 9.10E-09 41.67% GAWL01029301 ABO26596.1 perlucin 7 1092 5 1.80E-11 39.00% GAWL01032250 ABO26596.1 perlucin 7 1267 4 6.10E-10 42.50% GAWL01033150 ABO26596.1 perlucin 7 1733 1 1.90E-10 46.00% GAWL01033151 ABO26596.1 perlucin 7 1701 1 1.80E-10 44.00% GAWL01033154 ABO26596.1 perlucin 7 824 2 1.20E-11 42.00% GAWL01033494 ABO26596.1 perlucin 7 2261 6 1.40E-12 43.00% GAWL01033495 ABO26596.1 perlucin 7 2272 6 1.40E-12 43.00% GAWL01034205 ABO26596.1 perlucin 7 803 5 9.20E-17 46.00% GAWL01034206 ABO26596.1 perlucin 7 598 6 2.50E-15 44.00% GAWL01034407 ABO26596.1 perlucin 7 350 2 5.30E-11 44.50% GAWL01034782 ABO26596.1 perlucin 7 1861 5 6.10E-13 45.00% GAWL01034785 ABO26596.1 perlucin 7 1817 5 5.90E-13 45.00%
GAWL01036328 ABO26596.1 perlucin 7 3004 4 1.30E-10 46.00% GAWL01036329 ABO26596.1 perlucin 7 2977 4 1.10E-10 46.00% GAWL01036330 ABO26596.1 perlucin 7 2602 4 1.50E-10 46.00% GAWL01036332 ABO26596.1 perlucin 7 1845 5 1.60E-12 45.40% GAWL01036335 ABO26596.1 perlucin 7 2614 4 1.20E-10 46.00% GAWL01036336 ABO26596.1 perlucin 7 2641 4 1.20E-10 46.00% GAWL01038777 ABO26596.1 perlucin 7 413 2 3.40E-10 51.50% GAWL01038778 ABO26596.1 perlucin 7 585 2 1.00E-09 51.50% GAWL01041002 ABO26596.1 perlucin 7 796 5 2.80E-20 47.00% GAWL01041183 ABO26596.1 perlucin 7 468 5 7.30E-15 41.60% GAWL01005093 ABO26596.1 perlucin 7 303 3 2.60E-11 52.33% GAWL01011701 ADD16957.1 perlucin 7 515 7 1.70E-21 46.29% GAWL01017902 ADD16957.1 perlucin 7 605 2 9.90E-12 45.00% GAWL01001834 ADD16957.1 perlucin 7 545 2 1.30E-08 41.50% GAWL01033432 ABL63470.1 plasma membrane calcium atpase 3939 3 0.00E+00 57.00% GAWL01033433 ABL63470.1 plasma membrane calcium atpase 3972 3 0.00E+00 57.33% GAWL01033434 ABL63470.1 plasma membrane calcium atpase 2817 3 0.00E+00 56.33% GAWL01033435 ABL63470.1 plasma membrane calcium atpase 2850 3 0.00E+00 56.33% GAWL01015434 ADD16957.1 plc_halla ame: full=perlucin 788 4 9.70E-12 40.75% GAWL01015435 ADD16957.1 plc_halla ame: full=perlucin 813 4 1.10E-11 40.75% GAWL01023326 ADD16957.1 plc_halla ame: full=perlucin 850 5 1.70E-15 42.80% GAWL01027506 ADD16957.1 plc_halla ame: full=perlucin 1044 7 6.90E-17 45.57% GAWL01029186 ADD16957.1 plc_halla ame: full=perlucin 1774 2 8.30E-09 44.00% GAWL01032658 ADD16957.1 plc_halla ame: full=perlucin 709 2 1.30E-11 47.00% GAWL01032669 ADD16957.1 plc_halla ame: full=perlucin 551 7 2.30E-22 47.43% GAWL01035992 ADD16957.1 plc_halla ame: full=perlucin 1579 2 1.20E-10 39.50% GAWL01035982 ADD16957.1 plc_halla ame: full=perlucin 1534 2 1.20E-10 39.50% GAWL01040736 ADD16957.1 plc_halla ame: full=perlucin 476 5 6.90E-16 45.80% GAWL01005275 ADD16957.1 plc_halla ame: full=perlucin 630 6 2.80E-14 43.00%
GAWL01005347 P82596.3 plc_halla ame: full=perlucin 580 3 2.70E-10 42.00% GAWL01005480 P82596.3 plc_halla ame: full=perlucin 690 6 7.40E-20 44.17% GAWL01005810 P82596.3 plc_halla ame: full=perlucin 589 7 1.30E-21 46.86% GAWL01005831 P82596.3 plc_halla ame: full=perlucin 499 7 2.00E-18 44.57% GAWL01006141 P82596.3 plc_halla ame: full=perlucin 450 6 9.70E-19 43.17% GAWL01006160 P82596.3 plc_halla ame: full=perlucin 537 5 2.20E-18 44.00% GAWL01006190 P82596.3 plc_halla ame: full=perlucin 739 7 3.00E-18 44.43% GAWL01006557 P82596.3 plc_halla ame: full=perlucin 1353 7 4.10E-13 43.71% GAWL01006612 P82596.3 plc_halla ame: full=perlucin 566 3 2.00E-11 42.67% GAWL01006836 P82596.3 plc_halla ame: full=perlucin 537 4 5.20E-13 46.00% GAWL01007010 P82596.3 plc_halla ame: full=perlucin 1043 6 1.50E-15 45.17% GAWL01007457 P82596.3 plc_halla ame: full=perlucin 442 5 1.10E-13 41.80% GAWL01007748 P82596.3 plc_halla ame: full=perlucin 349 2 4.20E-11 47.00% GAWL01008118 P82596.3 plc_halla ame: full=perlucin 514 6 5.60E-17 44.33% GAWL01008327 P82596.3 plc_halla ame: full=perlucin 330 5 1.40E-14 51.40% GAWL01008500 P82596.3 plc_halla ame: full=perlucin 518 4 8.20E-13 41.00% GAWL01008862 P82596.3 plc_halla ame: full=perlucin 584 7 8.20E-24 45.57% GAWL01009030 P82596.3 plc_halla ame: full=perlucin 208 1 5.80E-07 48.00% GAWL01009239 P82596.3 plc_halla ame: full=perlucin 394 5 1.10E-13 47.60% GAWL01009321 P82596.3 plc_halla ame: full=perlucin 628 1 1.50E-07 47.00% GAWL01009948 P82596.3 plc_halla ame: full=perlucin 645 1 2.80E-09 45.00% GAWL01010243 P82596.3 plc_halla ame: full=perlucin 422 1 2.40E-10 55.00% GAWL01010364 P82596.3 plc_halla ame: full=perlucin 301 4 8.60E-10 46.50% GAWL01011095 P82596.3 plc_halla ame: full=perlucin 563 7 6.50E-17 41.57% GAWL01011139 P82596.3 plc_halla ame: full=perlucin 402 7 1.00E-16 44.71% GAWL01011399 P82596.3 plc_halla ame: full=perlucin 375 1 4.90E-09 53.00% GAWL01011400 P82596.3 plc_halla ame: full=perlucin 531 1 3.40E-07 48.00% GAWL01011500 P82596.3 plc_halla ame: full=perlucin 552 2 2.90E-10 40.50% GAWL01012148 P82596.3 plc_halla ame: full=perlucin 349 6 5.40E-16 46.00%
GAWL01012183 P82596.3 plc_halla ame: full=perlucin 381 2 3.50E-09 46.00% GAWL01012184 P82596.3 plc_halla ame: full=perlucin 367 3 1.20E-09 45.33% GAWL01013378 P82596.3 plc_halla ame: full=perlucin 635 7 6.60E-22 47.00% GAWL01013984 P82596.3 plc_halla ame: full=perlucin 497 1 1.00E-11 51.00% GAWL01014157 P82596.3 plc_halla ame: full=perlucin 255 2 7.90E-08 53.00% GAWL01014787 P82596.3 plc_halla ame: full=perlucin 219 3 2.80E-10 53.33% GAWL01015346 P82596.3 plc_halla ame: full=perlucin 335 1 3.50E-08 51.00% GAWL01015444 P82596.3 plc_halla ame: full=perlucin 619 7 4.40E-18 44.29% GAWL01015909 P82596.3 plc_halla ame: full=perlucin 665 3 3.00E-12 43.67% GAWL01015910 P82596.3 plc_halla ame: full=perlucin 629 4 1.40E-14 43.25% GAWL01016464 P82596.3 plc_halla ame: full=perlucin 754 2 5.00E-13 42.50% GAWL01016832 P82596.3 plc_halla ame: full=perlucin 558 7 4.10E-24 45.29% GAWL01016889 P82596.3 plc_halla ame: full=perlucin 528 7 1.60E-23 45.00% GAWL01017308 P82596.3 plc_halla ame: full=perlucin 888 1 9.60E-12 42.00% GAWL01017309 P82596.3 plc_halla ame: full=perlucin 867 2 6.60E-12 41.00% GAWL01017761 P82596.3 plc_halla ame: full=perlucin 432 3 2.90E-09 45.00% GAWL01018151 P82596.3 plc_halla ame: full=perlucin 1107 5 1.00E-11 42.60% GAWL01018326 P82596.3 plc_halla ame: full=perlucin 624 7 7.60E-21 46.00% GAWL01018595 P82596.3 plc_halla ame: full=perlucin 291 1 4.00E-07 45.00% GAWL01018983 P82596.3 plc_halla ame: full=perlucin 2211 7 4.10E-15 45.00% GAWL01019170 P82596.3 plc_halla ame: full=perlucin 538 6 8.90E-17 47.17% GAWL01019822 P82596.3 plc_halla ame: full=perlucin 630 6 2.60E-15 41.83% GAWL01019823 P82596.3 plc_halla ame: full=perlucin 533 7 1.10E-19 44.00% GAWL01020037 P82596.3 plc_halla ame: full=perlucin 872 1 7.10E-07 53.00% GAWL01020100 P82596.3 plc_halla ame: full=perlucin 538 7 1.30E-18 44.57% GAWL01020495 P82596.3 plc_halla ame: full=perlucin 567 7 4.20E-16 43.14% GAWL01020728 P82596.3 plc_halla ame: full=perlucin 683 3 1.90E-10 47.67% GAWL01020729 P82596.3 plc_halla ame: full=perlucin 704 3 2.50E-10 47.67% GAWL01022592 P82596.3 plc_halla ame: full=perlucin 255 1 7.50E-10 47.00%
GAWL01022681 P82596.3 plc_halla ame: full=perlucin 431 4 1.10E-18 46.25% GAWL01022682 P82596.3 plc_halla ame: full=perlucin 562 4 2.40E-18 46.25% GAWL01023311 P82596.3 plc_halla ame: full=perlucin 879 3 9.60E-11 41.33% GAWL01023325 P82596.3 plc_halla ame: full=perlucin 852 6 8.40E-12 41.83% GAWL01023395 P82596.3 plc_halla ame: full=perlucin 672 6 5.40E-22 46.67% GAWL01023523 P82596.3 plc_halla ame: full=perlucin 1785 3 1.60E-12 44.00% GAWL01023525 P82596.3 plc_halla ame: full=perlucin 1760 3 4.40E-13 43.67% GAWL01024350 P82596.3 plc_halla ame: full=perlucin 492 1 1.80E-07 55.00% GAWL01024548 P82596.3 plc_halla ame: full=perlucin 3672 2 2.50E-10 46.50% GAWL01024603 P82596.3 plc_halla ame: full=perlucin 893 2 1.40E-10 43.00% GAWL01024659 P82596.3 plc_halla ame: full=perlucin 213 4 6.00E-11 48.75% GAWL01024661 P82596.3 plc_halla ame: full=perlucin 274 1 1.20E-07 63.00% GAWL01024758 P82596.3 plc_halla ame: full=perlucin 549 7 7.80E-25 47.14% GAWL01024759 P82596.3 plc_halla ame: full=perlucin 733 7 4.60E-17 47.43% GAWL01025067 P82596.3 plc_halla ame: full=perlucin 1125 7 3.40E-18 45.57% GAWL01025068 P82596.3 plc_halla ame: full=perlucin 927 7 2.10E-18 45.43% GAWL01025071 P82596.3 plc_halla ame: full=perlucin 1112 7 3.20E-18 45.57% GAWL01025072 P82596.3 plc_halla ame: full=perlucin 1558 8 2.40E-16 44.25% GAWL01025140 P82596.3 plc_halla ame: full=perlucin 1026 6 1.60E-12 42.33% GAWL01025145 P82596.3 plc_halla ame: full=perlucin 1006 6 5.20E-19 46.00% GAWL01025147 P82596.3 plc_halla ame: full=perlucin 979 6 2.70E-19 46.17% GAWL01025412 P82596.3 plc_halla ame: full=perlucin 640 2 2.30E-11 44.50% GAWL01025441 P82596.3 plc_halla ame: full=perlucin 927 3 2.20E-09 45.67% GAWL01025702 P82596.3 plc_halla ame: full=perlucin 838 6 1.90E-17 46.00% GAWL01025712 P82596.3 plc_halla ame: full=perlucin 742 6 1.70E-18 46.00% GAWL01025720 P82596.3 plc_halla ame: full=perlucin 389 3 7.70E-15 49.33% GAWL01025722 P82596.3 plc_halla ame: full=perlucin 881 6 2.10E-15 46.50% GAWL01025724 P82596.3 plc_halla ame: full=perlucin 803 4 5.20E-16 50.50% GAWL01025726 P82596.3 plc_halla ame: full=perlucin 826 6 2.20E-17 46.00%
GAWL01025732 P82596.3 plc_halla ame: full=perlucin 754 6 1.30E-18 46.17% GAWL01025707 P82596.3 plc_halla ame: full=perlucin 797 6 1.30E-16 46.17% GAWL01027188 P82596.3 plc_halla ame: full=perlucin 1026 4 2.40E-11 46.75% GAWL01027189 P82596.3 plc_halla ame: full=perlucin 1571 4 1.10E-11 46.75% GAWL01028246 P82596.3 plc_halla ame: full=perlucin 1135 1 8.40E-09 45.00% GAWL01029026 P82596.3 plc_halla ame: full=perlucin 448 1 9.00E-08 52.00% GAWL01029029 P82596.3 plc_halla ame: full=perlucin 307 2 7.60E-12 48.00% GAWL01029153 P82596.3 plc_halla ame: full=perlucin 627 1 1.20E-08 44.00% GAWL01029161 P82596.3 plc_halla ame: full=perlucin 896 5 2.90E-15 43.00% GAWL01029162 P82596.3 plc_halla ame: full=perlucin 795 3 9.20E-13 43.33% GAWL01029184 P82596.3 plc_halla ame: full=perlucin 2438 2 9.40E-11 46.50% GAWL01029261 P82596.3 plc_halla ame: full=perlucin 834 4 7.20E-12 42.75% GAWL01029262 P82596.3 plc_halla ame: full=perlucin 1680 4 4.60E-11 42.75% GAWL01029263 P82596.3 plc_halla ame: full=perlucin 1662 4 1.20E-12 44.00% GAWL01029494 P82596.3 plc_halla ame: full=perlucin 629 7 5.00E-13 43.57% GAWL01029499 P82596.3 plc_halla ame: full=perlucin 770 7 1.30E-12 44.00% GAWL01029778 P82596.3 plc_halla ame: full=perlucin 264 1 1.10E-07 45.00% GAWL01029789 P82596.3 plc_halla ame: full=perlucin 206 1 2.60E-07 49.00% GAWL01030073 P82596.3 plc_halla ame: full=perlucin 416 2 4.60E-09 50.00% GAWL01030074 P82596.3 plc_halla ame: full=perlucin 442 2 7.30E-09 48.50% GAWL01030079 P82596.3 plc_halla ame: full=perlucin 310 2 5.40E-13 49.50% GAWL01030080 P82596.3 plc_halla ame: full=perlucin 446 5 2.00E-09 44.20% GAWL01030167 P82596.3 plc_halla ame: full=perlucin 674 6 3.10E-19 43.17% GAWL01030229 P82596.3 plc_halla ame: full=perlucin 1773 5 4.40E-16 45.20% GAWL01030231 P82596.3 plc_halla ame: full=perlucin 1530 5 3.20E-16 45.20% GAWL01030223 P82596.3 plc_halla ame: full=perlucin 1019 5 7.20E-17 45.20% GAWL01030226 P82596.3 plc_halla ame: full=perlucin 1262 5 1.50E-16 45.20% GAWL01031304 P82596.3 plc_halla ame: full=perlucin 1041 1 5.30E-07 43.00% GAWL01032646 P82596.3 plc_halla ame: full=perlucin 596 5 1.80E-12 44.40%
GAWL01032656 P82596.3 plc_halla ame: full=perlucin 376 4 1.90E-12 50.50% GAWL01032657 P82596.3 plc_halla ame: full=perlucin 274 3 1.00E-09 53.00% GAWL01032659 P82596.3 plc_halla ame: full=perlucin 226 1 1.80E-07 55.00% GAWL01032660 P82596.3 plc_halla ame: full=perlucin 562 7 5.80E-20 46.86% GAWL01032661 P82596.3 plc_halla ame: full=perlucin 564 5 3.10E-12 44.60% GAWL01032662 P82596.3 plc_halla ame: full=perlucin 552 5 7.20E-12 43.60% GAWL01032664 P82596.3 plc_halla ame: full=perlucin 572 5 1.70E-12 44.40% GAWL01032647 P82596.3 plc_halla ame: full=perlucin 512 1 6.20E-07 53.00% GAWL01032667 P82596.3 plc_halla ame: full=perlucin 281 6 3.70E-15 48.83% GAWL01032668 P82596.3 plc_halla ame: full=perlucin 620 7 9.10E-22 48.00% GAWL01032649 P82596.3 plc_halla ame: full=perlucin 314 6 3.90E-11 53.67% GAWL01032651 P82596.3 plc_halla ame: full=perlucin 560 4 3.90E-12 44.75% GAWL01032654 P82596.3 plc_halla ame: full=perlucin 584 4 4.00E-12 44.75% GAWL01032716 P82596.3 plc_halla ame: full=perlucin 944 1 6.30E-07 44.00% GAWL01032872 P82596.3 plc_halla ame: full=perlucin 294 3 1.40E-12 54.67% GAWL01033357 P82596.3 plc_halla ame: full=perlucin 969 3 5.80E-12 42.67% GAWL01033360 P82596.3 plc_halla ame: full=perlucin 1384 2 3.40E-12 44.00% GAWL01033362 P82596.3 plc_halla ame: full=perlucin 745 2 6.50E-13 45.00% GAWL01033363 P82596.3 plc_halla ame: full=perlucin 1436 3 1.40E-09 42.00% GAWL01033392 P82596.3 plc_halla ame: full=perlucin 352 1 2.40E-11 48.00% GAWL01033403 P82596.3 plc_halla ame: full=perlucin 560 5 6.20E-11 47.40% GAWL01033406 P82596.3 plc_halla ame: full=perlucin 595 4 7.50E-11 47.75% GAWL01033408 P82596.3 plc_halla ame: full=perlucin 702 3 2.50E-10 47.33% GAWL01033394 P82596.3 plc_halla ame: full=perlucin 702 3 2.60E-10 47.33% GAWL01033398 P82596.3 plc_halla ame: full=perlucin 894 3 5.30E-10 47.33% GAWL01034413 P82596.3 plc_halla ame: full=perlucin 445 1 2.30E-07 41.00% GAWL01034414 P82596.3 plc_halla ame: full=perlucin 632 6 3.50E-15 41.33% GAWL01034617 P82596.3 plc_halla ame: full=perlucin 733 2 4.10E-09 45.00% GAWL01035031 P82596.3 plc_halla ame: full=perlucin 688 4 1.00E-13 44.75%
GAWL01035200 P82596.3 plc_halla ame: full=perlucin 368 3 5.40E-11 49.67% GAWL01035665 P82596.3 plc_halla ame: full=perlucin 469 1 8.40E-07 49.00% GAWL01036337 P82596.3 plc_halla ame: full=perlucin 2587 1 3.40E-07 48.00% GAWL01036333 P82596.3 plc_halla ame: full=perlucin 2224 1 4.40E-07 45.00% GAWL01036334 P82596.3 plc_halla ame: full=perlucin 2212 1 6.90E-09 48.00% GAWL01036730 P82596.3 plc_halla ame: full=perlucin 1922 5 1.10E-11 42.80% GAWL01036731 P82596.3 plc_halla ame: full=perlucin 1796 5 9.90E-12 42.80% GAWL01036732 P82596.3 plc_halla ame: full=perlucin 1924 5 1.00E-11 42.80% GAWL01036734 P82596.3 plc_halla ame: full=perlucin 1824 5 1.00E-11 42.80% GAWL01036735 P82596.3 plc_halla ame: full=perlucin 1809 5 1.00E-11 42.80% GAWL01036736 P82596.3 plc_halla ame: full=perlucin 2064 4 1.90E-11 43.50% GAWL01036737 P82596.3 plc_halla ame: full=perlucin 1811 5 1.00E-11 42.80% GAWL01036726 P82596.3 plc_halla ame: full=perlucin 1951 4 1.90E-11 43.50% GAWL01036728 P82596.3 plc_halla ame: full=perlucin 1937 5 1.00E-11 42.80% GAWL01036729 P82596.3 plc_halla ame: full=perlucin 1909 5 1.10E-11 42.80% GAWL01037310 P82596.3 plc_halla ame: full=perlucin 889 3 2.70E-11 43.67% GAWL01037311 P82596.3 plc_halla ame: full=perlucin 727 3 1.50E-11 43.67% GAWL01037313 P82596.3 plc_halla ame: full=perlucin 489 3 1.70E-12 44.33% GAWL01037303 P82596.3 plc_halla ame: full=perlucin 1081 3 4.00E-11 43.67% GAWL01037309 P82596.3 plc_halla ame: full=perlucin 1295 3 5.50E-11 43.67% GAWL01037387 P82596.3 plc_halla ame: full=perlucin 329 2 2.00E-09 46.50% GAWL01037390 P82596.3 plc_halla ame: full=perlucin 246 2 1.10E-10 47.00% GAWL01037394 P82596.3 plc_halla ame: full=perlucin 383 3 4.60E-11 45.00% GAWL01037399 P82596.3 plc_halla ame: full=perlucin 582 1 3.20E-11 53.00% GAWL01037401 P82596.3 plc_halla ame: full=perlucin 258 2 1.70E-10 46.50% GAWL01037462 P82596.3 plc_halla ame: full=perlucin 2819 5 1.50E-15 45.60% GAWL01039232 P82596.3 plc_halla ame: full=perlucin 2078 1 8.20E-10 46.00% GAWL01039223 P82596.3 plc_halla ame: full=perlucin 2417 3 2.20E-14 41.67% GAWL01039228 P82596.3 plc_halla ame: full=perlucin 1285 6 1.60E-14 42.83%
GAWL01039230 P82596.3 plc_halla ame: full=perlucin 3640 1 6.30E-08 43.00% GAWL01039567 P82596.3 plc_halla ame: full=perlucin 1800 5 4.20E-15 45.60% GAWL01039561 P82596.3 plc_halla ame: full=perlucin 1132 1 3.50E-08 43.00% GAWL01039562 P82596.3 plc_halla ame: full=perlucin 1825 5 4.10E-15 45.00% GAWL01039942 P82596.3 plc_halla ame: full=perlucin 862 4 6.00E-12 42.25% GAWL01039949 P82596.3 plc_halla ame: full=perlucin 1310 4 2.30E-11 42.50% GAWL01039955 P82596.3 plc_halla ame: full=perlucin 2061 4 4.50E-11 42.50% GAWL01040005 P82596.3 plc_halla ame: full=perlucin 3161 1 2.60E-10 53.00% GAWL01040014 P82596.3 plc_halla ame: full=perlucin 697 1 1.40E-09 50.00% GAWL01040017 P82596.3 plc_halla ame: full=perlucin 288 1 9.90E-07 46.00% GAWL01040019 P82596.3 plc_halla ame: full=perlucin 3173 1 1.30E-08 50.00% GAWL01039998 P82596.3 plc_halla ame: full=perlucin 276 1 8.10E-09 48.00% GAWL01040001 P82596.3 plc_halla ame: full=perlucin 685 2 1.60E-11 45.50% GAWL01040059 P82596.3 plc_halla ame: full=perlucin 1279 2 1.60E-07 41.50% GAWL01040060 P82596.3 plc_halla ame: full=perlucin 1261 2 1.60E-07 41.50% GAWL01040061 P82596.3 plc_halla ame: full=perlucin 1174 2 1.50E-07 41.50% GAWL01041133 P82596.3 plc_halla ame: full=perlucin 517 1 8.20E-10 46.00% GAWL01041471 P82596.3 plc_halla ame: full=perlucin 688 7 8.80E-21 44.57% GAWL01041836 P82596.3 plc_halla ame: full=perlucin 477 7 1.40E-24 47.86% GAWL01042285 P82596.3 plc_halla ame: full=perlucin 454 4 8.00E-14 47.25% GAWL01042962 P82596.3 plc_halla ame: full=perlucin 218 1 9.20E-07 47.00% GAWL01043044 P82596.3 plc_halla ame: full=perlucin 321 2 5.70E-11 55.50% GAWL01043334 P82596.3 plc_halla ame: full=perlucin 362 4 2.80E-11 48.25% GAWL01044009 P82596.3 plc_halla ame: full=perlucin 240 1 1.80E-07 48.00% GAWL01044290 P82596.3 plc_halla ame: full=perlucin 241 2 7.70E-08 47.00% GAWL01000825 P82596.3 plc_halla ame: full=perlucin 360 6 8.50E-21 46.33% GAWL01000833 P82596.3 plc_halla ame: full=perlucin 276 4 2.80E-11 50.50% GAWL01000834 P82596.3 plc_halla ame: full=perlucin 261 3 4.00E-11 50.67% GAWL01000956 P82596.3 plc_halla ame: full=perlucin 521 7 2.40E-19 42.71%
GAWL01001767 P82596.3 plc_halla ame: full=perlucin 508 6 1.20E-18 45.50% GAWL01001859 P82596.3 plc_halla ame: full=perlucin 1307 2 3.30E-11 40.00% GAWL01002083 P82596.3 plc_halla ame: full=perlucin 701 1 9.50E-12 50.00% GAWL01002281 P82596.3 plc_halla ame: full=perlucin 301 6 1.10E-12 49.33% GAWL01002295 P82596.3 plc_halla ame: full=perlucin 587 7 3.80E-19 45.00% GAWL01002403 P82596.3 plc_halla ame: full=perlucin 1122 3 1.10E-10 44.00% GAWL01003008 P82596.3 plc_halla ame: full=perlucin 385 1 8.30E-07 55.00% GAWL01003137 P82596.3 plc_halla ame: full=perlucin 638 7 3.10E-19 45.29% GAWL01003334 P82596.3 plc_halla ame: full=perlucin 629 3 6.50E-10 42.33% GAWL01003381 P82596.3 plc_halla ame: full=perlucin 314 4 2.50E-11 51.75% GAWL01003468 P82596.3 plc_halla ame: full=perlucin 808 7 2.00E-18 45.14% GAWL01004537 P82596.3 plc_halla ame: full=perlucin 307 4 8.50E-12 49.75% GAWL01005212 P82596.3 plc_halla ame: full=perlucin 711 3 1.70E-15 42.33% GAWL01021489 P84811.1 pwap_halla ame: full=perlwapin 323 1 6.70E-07 55.00% GAWL01005863 ABS19815.1 sarco endoplasmic reticulum calcium atpase isoform a 5424 3 0.00E+00 75.33% GAWL01026599 ABS19815.1 sarco endoplasmic reticulum calcium atpase isoform a 3567 3 7.60E-93 47.67% GAWL01026600 ABS19815.1 sarco endoplasmic reticulum calcium atpase isoform a 1501 3 7.10E-40 55.33% GAWL01004710 ABX57736.1 tfg beta signaling pathway factor 384 1 9.70E-13 84.00% GAWL01031629 BAF42771.1 tyrosinase-like protein 1 813 3 1.10E-37 47.33% GAWL01031630 BAF42771.1 tyrosinase-like protein 1 2403 3 3.20E-35 48.00% GAWL01031631 BAF42771.1 tyrosinase-like protein 1 2430 3 3.30E-35 48.00% GAWL01031633 BAF42771.1 tyrosinase-like protein 1 2403 3 3.20E-35 48.00% GAWL01031635 BAF42771.1 tyrosinase-like protein 1 2430 3 3.30E-35 48.00% GAWL01004918 BAF42772.1 tyrosinase-like protein 2 390 3 8.20E-14 45.67% Differentially or highly expressed in Crassostrea gigas GAWL01018228 JH815923.1 mantle tissue 2008 1 1.00E-30 83.00% Differentially or highly expressed in Crassostrea gigas GAWL01017748 JH816073.1 mantle tissue 979 1 1.40E-21 85.00% Differentially or highly expressed in Crassostrea gigas GAWL01045091 JH816168.1 mantle tissue 307 1 1.10E-27 80.00% GAWL01000001 JH816168.1 Differentially or highly expressed in Crassostrea gigas 207 1 4.20E-40 81.00%
mantle tissue Differentially or highly expressed in Crassostrea gigas GAWL01012116 JH816189.1 mantle tissue 349 1 4.50E-42 91.00% Differentially or highly expressed in Crassostrea gigas GAWL01012117 JH816189.1 mantle tissue 766 1 8.60E-167 80.00% Differentially or highly expressed in Crassostrea gigas GAWL01019644 JH816189.1 mantle tissue 676 1 3.60E-150 80.00% Differentially or highly expressed in Crassostrea gigas GAWL01024987 JH816189.1 mantle tissue 979 3 1.10E-146 78.00% Differentially or highly expressed in Crassostrea gigas GAWL01019441 JH816585.1 mantle tissue 505 1 1.90E-12 76.00% Differentially or highly expressed in Crassostrea gigas GAWL01019442 JH816585.1 mantle tissue 487 1 1.90E-12 76.00% Differentially or highly expressed in Crassostrea gigas GAWL01012989 JH816769.1 mantle tissue 307 2 3.80E-62 82.00% Differentially or highly expressed in Crassostrea gigas GAWL01021618 JH816843.1 mantle tissue 2885 1 3.10E-32 84.00% Differentially or highly expressed in Crassostrea gigas GAWL01007983 JH816852.1 mantle tissue 1202 1 1.00E-23 93.00% Differentially or highly expressed in Crassostrea gigas GAWL01012990 JH816866.1 mantle tissue 307 2 2.30E-69 82.50% Differentially or highly expressed in Crassostrea gigas GAWL01011674 JH816980.1 mantle tissue 447 1 3.30E-84 85.00% Differentially or highly expressed in Crassostrea gigas GAWL01026283 JH816980.1 mantle tissue 639 1 5.10E-39 80.00% Differentially or highly expressed in Crassostrea gigas GAWL01003177 JH817066.1 mantle tissue 373 1 1.10E-23 85.00% Differentially or highly expressed in Crassostrea gigas GAWL01024988 JH817078.1 mantle tissue 939 1 1.60E-75 76.00% Differentially or highly expressed in Crassostrea gigas GAWL01038785 JH817078.1 mantle tissue 2093 3 0.00E+00 81.67% Differentially or highly expressed in Crassostrea gigas GAWL01038794 JH817078.1 mantle tissue 2036 3 0.00E+00 81.67% Differentially or highly expressed in Crassostrea gigas GAWL01038795 JH817078.1 mantle tissue 2489 3 0.00E+00 82.00% Differentially or highly expressed in Crassostrea gigas GAWL01038787 JH817078.1 mantle tissue 768 2 1.50E-134 85.00%
Differentially or highly expressed in Crassostrea gigas GAWL01038788 JH817078.1 mantle tissue 1851 3 0.00E+00 82.00% Differentially or highly expressed in Crassostrea gigas GAWL01038789 JH817078.1 mantle tissue 1951 3 0.00E+00 81.67% Differentially or highly expressed in Crassostrea gigas GAWL01038791 JH817078.1 mantle tissue 2159 3 0.00E+00 82.00% Differentially or highly expressed in Crassostrea gigas GAWL01038793 JH817078.1 mantle tissue 865 2 2.90E-127 84.00% Differentially or highly expressed in Crassostrea gigas GAWL01018747 JH817228.1 mantle tissue 3644 1 3.10E-13 89.00% Differentially or highly expressed in Crassostrea gigas GAWL01006995 JH817484.1 mantle tissue 1707 1 6.80E-07 92.00% Differentially or highly expressed in Crassostrea gigas GAWL01013936 JH817484.1 mantle tissue 379 1 2.30E-30 89.00% Differentially or highly expressed in Crassostrea gigas GAWL01002045 JH817532.1 mantle tissue 356 1 1.30E-12 100.00% Differentially or highly expressed in Crassostrea gigas GAWL01002046 JH817532.1 mantle tissue 307 1 1.10E-12 100.00% Differentially or highly expressed in Crassostrea gigas GAWL01040644 JH817704.1 mantle tissue 1214 1 3.70E-08 92.00% Differentially or highly expressed in Crassostrea gigas GAWL01016929 JH817738.1 mantle tissue 1148 1 1.20E-22 84.00% Differentially or highly expressed in Crassostrea gigas GAWL01011647 JH817782.1 mantle tissue 1221 1 8.00E-15 82.00% Differentially or highly expressed in Crassostrea gigas GAWL01012118 JH817877.1 mantle tissue 402 2 5.40E-27 83.00% Differentially or highly expressed in Crassostrea gigas GAWL01016301 JH817892.1 mantle tissue 2254 1 9.00E-12 89.00% Differentially or highly expressed in Crassostrea gigas GAWL01028947 JH818313.1 mantle tissue 1372 1 0.00E+00 79.00% Differentially or highly expressed in Crassostrea gigas GAWL01028957 JH818313.1 mantle tissue 1871 1 0.00E+00 84.00% Differentially or highly expressed in Crassostrea gigas GAWL01028959 JH818313.1 mantle tissue 1981 1 0.00E+00 79.00% Differentially or highly expressed in Crassostrea gigas GAWL01028961 JH818313.1 mantle tissue 1026 1 0.00E+00 84.00% Differentially or highly expressed in Crassostrea gigas GAWL01028964 JH818313.1 mantle tissue 1626 1 0.00E+00 78.00%
Differentially or highly expressed in Crassostrea gigas GAWL01028965 JH818313.1 mantle tissue 1928 1 0.00E+00 79.00% Differentially or highly expressed in Crassostrea gigas GAWL01028948 JH818313.1 mantle tissue 1386 1 0.00E+00 79.00% Differentially or highly expressed in Crassostrea gigas GAWL01028966 JH818313.1 mantle tissue 1558 1 0.00E+00 84.00% Differentially or highly expressed in Crassostrea gigas GAWL01028967 JH818313.1 mantle tissue 1967 1 0.00E+00 79.00% Differentially or highly expressed in Crassostrea gigas GAWL01028969 JH818313.1 mantle tissue 1615 1 0.00E+00 83.00% Differentially or highly expressed in Crassostrea gigas GAWL01028970 JH818313.1 mantle tissue 1615 1 0.00E+00 79.00% Differentially or highly expressed in Crassostrea gigas GAWL01028949 JH818313.1 mantle tissue 1036 1 3.00E-38 82.00% Differentially or highly expressed in Crassostrea gigas GAWL01028950 JH818313.1 mantle tissue 1884 1 0.00E+00 85.00% Differentially or highly expressed in Crassostrea gigas GAWL01028951 JH818313.1 mantle tissue 1571 1 0.00E+00 84.00% Differentially or highly expressed in Crassostrea gigas GAWL01028952 JH818313.1 mantle tissue 1039 1 0.00E+00 84.00% Differentially or highly expressed in Crassostrea gigas GAWL01028953 JH818313.1 mantle tissue 1928 1 0.00E+00 84.00% Differentially or highly expressed in Crassostrea gigas GAWL01028954 JH818313.1 mantle tissue 1612 1 0.00E+00 78.00% Differentially or highly expressed in Crassostrea gigas GAWL01028955 JH818313.1 mantle tissue 1083 1 2.60E-178 83.00% Differentially or highly expressed in Crassostrea gigas GAWL01002076 JH818820.1 mantle tissue 3432 1 1.30E-36 85.00% Differentially or highly expressed in Crassostrea gigas GAWL01001009 JH818839.1 mantle tissue 1082 1 2.60E-09 77.00% 950
951