HOLLAND LANDING PRAIRIE PROVINCIAL PARK One Malaise trap was deployed at Holland Landing Prairie Provincial Park in 2014 (44.11894, -79.48795, 271m ASL; Figure 1). This trap collected arthropods for twenty weeks from May 1 – September 22, 2014. All 10 Malaise trap samples were processed using the bulk sample analysis protocol. A total of 2345 BINs were obtained. Over half the BINs captured were flies (Diptera), followed by bees, ants and wasps (Hymenoptera), moths and butterflies (Lepidoptera), and true bugs (Hemiptera; Figure 2). In total, 544 arthropod species were named, representing 26.1% of the BINs from the site (Appendix 1). All the BINs were assigned at least to family, and 60.5% were assigned to a genus Figure 1. Malaise trap deployed at Holland Landing (Appendix 2). Specimens collected from Holland Prairie Provincial Park in 2014. Landing Prairie represent 165 different families and 623 genera. Figure 2. Taxonomy breakdown of BINs captured in the Malaise trap at Holland Landing Prairie. APPENDIX 1. TAXONOMY REPORT Class Order Family Genus Species Arachnida Araneae Theridiidae Theridion Theridion murarium Diplopoda Julida Julidae Insecta Psocodea Amphipsocidae Polypsocus Polypsocus corruptus Caeciliusidae Valenzuela Valenzuela burmeisteri Valenzuela flavidus Valenzuela nadleri Ectopsocidae Ectopsocus Ectopsocus meridionalis Lachesillidae Lachesilla Lepidopsocidae Echmepteryx Echmepteryx hageni Peripsocidae Peripsocus Peripsocus madidus Peripsocus subfasciatus Psocidae Cerastipsocus Trichadenotecnum Trichadenotecnum alexanderae complex Trichadenotecnum slossonae Stenopsocidae Graphopsocus Graphopsocus cruciatus Hymenoptera Apidae Bombus Bombus impatiens Page 1 of 32 Class Order Family Genus Species Ceratina Nomada Bethylidae Braconidae Acrisis Aleiodes Aliolus Apanteles Apanteles morrisi Austrozele Bracon Centistes Chelonus Choeras Clinocentrus Dinotrema Diolcogaster Dolichogenidea Ephedrus Ephedrus lacertosus Euphoriella Glyptapanteles Heterospilus Macrocentrus Meteorus Meteorus ictericus Meteorus trachynotus Microgaster Microplitis Microplitis scutellatus Mirax Orgilus Orthostigma Phanerotoma Pholetesor Pholetesor ornigis Praon Protapanteles Rhysipolis Taphaeus Wesmaelia Chrysididae Chrysis Page 2 of 32 Class Order Family Genus Species Chrysis cembricola Crabronidae Ectemnius Ectemnius cephalotes Mimesa Mimesa ezra Passaloecus Passaloecus eremita Psenulus Psenulus trisulcus Stigmus Trypoxylon Diapriidae Pantoclis Pantoclis hirtistilus Paramesius Diprionidae Gilpinia Gilpinia hercyniae Dryinidae Figitidae Ganaspis Ganaspis seticornis Formicidae Lasius Lasius alienus Halictidae Lasioglossum Lasioglossum lineatulum Lasioglossum nigroviride Lasioglossum timothyi Sphecodes Sphecodes ranunculi Ichneumonidae Agrothereutes Agrothereutes abbreviatus Agrypon Apechthis Apechthis annulicornis Aperileptus Aperileptus albipalpus Campodorus Campoletis Page 3 of 32 Class Order Family Genus Species Campoplex Campoplex difformis Cidaphus Cidaphus paniscoides Coelichneumon Coelichneumon navus Colpotrochia Colpotrochia crassipes Delomerista Delomerista novita Dialipsis Dialipsis dissimilis Dusona Echthrus Enclisis Enicospilus Erigorgus Erigorgus sinuosus Euceros Euceros flavescens Exenterus Exenterus vellicatus Exochus Exochus albifrons Glypta Glypta exartemae Hercus Hercus fontinalis Hypsicera Ichneumon Ichneumon memorator Ichneumon validicornis Ischnus Ischnus alternator Leptocampoplex Leptocampoplex cremastoides Lissonota Lissonota sexcincta Mesochorus Mesochorus discitergus Netelia Ophion Ophion idoneus Page 4 of 32 Class Order Family Genus Species Orthocentrus Oxytorus Oxytorus antennatus Phobocampe Phobocampe bicingulata Pimpla Plectiscus Plectiscus callidulus Pleolophus Podoschistus Podoschistus vittifrons Proclitus Proclitus praetor Scambus Sphecophaga Sphecophaga vesparum Stenomacrus Sussaba Sussaba cognata Therion Therion californicum Thyreodon Trychosis Tycherus Zatypota Zatypota cingulata Megaspilidae Mymaridae Anagrus Platygastridae Telenomus Telenomus coloradensis Trichacis Pompilidae Ceropales Ceropales maculata Priocnemis Priocnemis germana Pteromalidae Cecidostiba Tenthredinidae Aneugmenus Aneugmenus flavipes Page 5 of 32 Class Order Family Genus Species Macrophya Macrophya varia Nematus Pristiphora Vespidae Dolichovespula Dolichovespula arenaria Vespula Vespula flavopilosa Vespula maculifrons Vespula vidua Xyelidae Xyela Diptera Chironomidae Bryophaenocladius Bryophaenocladius ictericus Chironomus Chironomus acidophilus Chironomus bifurcatus Chironomus maturus Chironomus melanescens Cladopelma Conchapelopia Conchapelopia telema Corynoneura Corynoneura scutellata Cricotopus Cricotopus triannulatus Cricotopus trifascia Cricotopus vierriensis Dicrotendipes Dicrotendipes modestus Dicrotendipes tritomus Einfeldia Gymnometriocnemus Gymnometriocnemus brumalis Heleniella Heterotrissocladius Heterotrissocladius changi Krenopelopia Limnophyes Limnophyes asquamatus Page 6 of 32 Class Order Family Genus Species Limnophyes sp. 14ES Metriocnemus Micropsectra Micropsectra recurvata Micropsectra subletteorum Micropsectra xantha Microtendipes Microtendipes pedellus Monopelopia Monopelopia tenuicalcar Orthocladius Parachironomus Parakiefferiella Parametriocnemus Paraphaenocladius Paraphaenocladius impensus Paratanytarsus Paratanytarsus laccophilus Paratanytarsus sp. 7TE Phaenopsectra Polypedilum Procladius Smittia Smittia sp. 8ES Stempellinella Tanytarsus Tanytarsus mendax Cecidomyiidae Asphondylia Asteromyia Contarinia Dasineura Feltiella Janetiella Miastor Miastor metraloas Orseolia Peromyia Peromyia borealis Resseliella Rhopalomyia Sitodiplosis Vitisiella Page 7 of 32 Class Order Family Genus Species Vitisiella brevicauda Agromyzidae Aulagromyza Cerodontha Hexomyza Hexomyza schineri Phytobia Phytomyza Anisopodidae Mycetobia Sylvicola Sylvicola fuscatus Anthomyiidae Anthomyia Chirosia Chirosia stratifrons Delia Egle Eustalomyia Eutrichota Eutrichota lipsia Hydrophoria Hydrophoria lancifer Pegomya Pegomya geniculata Pegoplata Pegoplata nigroscutellata Asilidae Laphria Machimus Machimus notatus Ommatius Ommatius tibialis Aulacigastridae Aulacigaster Aulacigaster mcalpinei Aulacigaster neoleucopeza Bombyliidae Bombylius Bombylius major Bombylius pygmaeus Calliphoridae Calliphora Page 8 of 32 Class Order Family Genus Species Calliphora livida Cynomya Phormia Phormia regina Pollenia Pollenia pediculata Pollenia vagabunda Ceratopogonidae Alluaudomyia Alluaudomyia parva Atrichopogon Bezzia Ceratopogon Culicoides Culicoides biguttatus Culicoides scanloni Culicoides travisi Dasyhelea Dasyhelea arenivaga Forcipomyia Forcipomyia bipunctata Forcipomyia ciliata Monohelea Palpomyia Stilobezzia Chaoboridae Chaoborus Chaoborus punctipennis Chloropidae Chaetochlorops Chaetochlorops inquilinus Elachiptera Elachiptera nigriceps Gaurax Gaurax dubius Gaurax pallidipes Gaurax shannoni Incertella Incertella incerta Psilacrum Psilacrum arpidia Tricimba Tricimba melancholica Page 9 of 32 Class Order Family Genus Species Tricimba trisulcata Clusiidae Clusiodes Clusiodes ater Clusiodes johnsoni Sobarocephala Sobarocephala atricornis Culicidae Aedes Aedes cinereus Coquillettidia Coquillettidia perturbans Culex Culex territans Culiseta Culiseta morsitans Diadocidiidae Diastatidae Campichoeta Campichoeta griseola Ditomyiidae Symmerus Dolichopodidae Chrysotimus Chrysotimus luteopalpus Chrysotus Condylostylus Dolichopus Gymnopternus Hercostomus Hercostomus unicolor Medetera Medetera vittata Neurigona Neurigona tenuis Sympycnus Sympycnus lineatus Xanthochlorus Xanthochlorus helvinus Drosophilidae Drosophila Drosophila neotestacea Drosophila robusta Page 10 of 32 Class Order Family Genus Species Drosophila tripunctata Mycodrosophila Mycodrosophila dimidiata Scaptomyza Scaptomyza pallida Dryomyzidae Dryomyza Dryomyza anilis Empididae Chelipoda Chelipoda truncata Empis Empis colonica Rhamphomyia Ephydridae Philygria Philygria debilis Psilopa Psilopa leucostoma Fanniidae Fannia Fannia armata Fannia sociella Heleomyzidae Suillia Suillia longipennis Suillia quinquepunctata Hybotidae Allodromia Allodromia testacea Leptopeza Megagrapha Megagrapha platytarsis Platypalpus Platypalpus melleus Iteaphila-group Iteaphila Keroplatidae Orfelia Orfelia nemoralis Lauxaniidae Homoneura Minettia Page 11 of 32 Class Order Family Genus Species Poecilolycia Poecilominettia Poecilominettia puncticeps Limoniidae Dicranomyia Dicranomyia frontalis Elephantomyia Elephantomyia westwoodi Erioptera Erioptera chlorophylla Metalimnobia Metalimnobia immatura Ormosia Ormosia affinis Rhipidia Lonchaeidae Lonchaea Milichiidae Milichia Paramyia Muscidae Coenosia Dasyphora Dasyphora cyanicolor Helina Helina evecta Helina rufitibia Muscina Muscina pascuorum Mydaea Mydaea flavicornis Phaonia Phaonia aberrans Phaonia errans Phaonia fuscana Potamia Potamia littoralis Thricops Thricops diaphanus Mycetophilidae Acnemia Acomoptera Allodia Page 12 of 32 Class Order Family Genus Species Azana Boletina Brevicornu Clastobasis Cordyla Docosia Epicypta Exechia Greenomyia Leia Leptomorphus Leptomorphus hyalinus Monoclona Monoclona rufilatera Mycetophila Mycetophila fungorum Mycetophila stylata Mycetophila uninotata Mycomya Palaeodocosia Phronia Phronia tenuis Sceptonia Sceptonia nigra Sciophila Syntemna Tetragoneura Xenexechia Zygomyia Phoridae Chaetopleurophora Chaetopleurophora multiseriata Gymnophora Gymnophora subarcuata Hypocera Lecanocerus Lecanocerus compressiceps Megaselia Megaselia arcticae Megaselia aristalis Megaselia citrinella Megaselia fungivora Megaselia lucifrons Page 13 of 32 Class Order Family