ANNUAL REPORT April 2009 to March 2010

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ANNUAL REPORT April 2009 to March 2010 Ôããè ¡ãè †¹ãŠ ¡ãè CDFD ÌãããäÓãÇ㊠¹ãÆãä¦ãÌãñª¶ã ‚ã¹ãÆõÊã 2009 Ôãñ ½ããÞãà 2010 ¦ã‡ãŠ ANNUAL REPORT April 2009 to March 2010 ¡ãè †¶ã † ãä¹ãâŠØãÀãä¹ãÆâãä›âØã †Ìãâ ãä¶ãªã¶ã ‡ãñŠ¶³ ¶ãã½ã¹ãÊÊããè, ÖõªÀãºã㪠- 500 001 Centre for DNA Fingerprinting and Diagnostics Nampally, Hyderabad - 500 001 1 2 CONTENTS I Mandate 5 II From the Director’s Desk 9 III Services 1. Laboratory of DNA Fingerprinting Services 15 2. Diagnostic Services 18 IV Research 1. Laboratory of Molecular Genetics 27 2. Laboratory of Genomics and Profiling Applications 35 3. Laboratory of Fungal Pathogenesis 40 4. Laboratory of Immunology 44 5. Laboratory of Bacterial Genetics 48 6. Laboratory of Computational Biology 53 7. Laboratory of Molecular Cell Biology 57 8. Laboratory of Structural Biology 61 9. Laboratory of Mammalian Genetics 65 10. Laboratory of Molecular Oncology 69 11. Laboratory of Cancer Biology 74 12. Laboratory of Computational and Functional Genomics 78 13. Laboratory of Transcription 83 14. Laboratory of Cell Signalling 87 15. Laboratory of Plant Microbe Interaction 91 16. Other Scientific Services / Facilities a. Bioinformatics 97 b. Instrumentation 98 V Publications 99 VI Human Resource Development 107 VII Lectures, Meetings, Workshops, and Important Events 111 VIII Senior Staff and Officers of CDFD 115 IX Deputations Abroad of CDFD Personnel 119 X Committees of the Institute 123 XI Budget and Finance 133 XII Auditor’s Report 137 XIII Photo Gallery 227 3 4 Mandate 5 6 MANDATE The objectives for which the Centre for DNA Fingerprinting and Diagnostics (CDFD) was established as enumerated in Memorandum of Association and Rules and Regulations of CDFD Society are as follows: i. To carry out scientific research pertaining to DNA profiling and related analysis in civil cases like paternity disputes, immigration, and exchange of newborns in hospitals, for various agencies including private parties, on appropriate payment; ii. To provide DNA fingerprinting and related analysis and facilities to crime investigation agencies; iii. To assist police personnel, forensic scientists, lawyers and the judiciary in understanding the evidential value of the DNA profile analysis and related techniques in crime investigation and family matters; iv. To establish DNA diagnostic methods for detecting genetic disorders and to develop probes for such detection; v. To use DNA fingerprinting techniques for the authentication of plant and animal cell material, cell lines and to develop new probes where necessary for such purposes; vi. To provide training in DNA fingerprinting techniques; vii. To undertake basic, applied and developmental R & D work; viii. To provide consultancy services to medical institutions, public health agencies and industry in the country; ix. To collaborate with foreign research institutions and laboratories and other international organizations in fields relevant to the objectives of the Centre; x. To establish affiliation with recognized universities and institutions of higher learning for the purpose of enabling research scholars to register for post-graduate degrees; xi. To receive grants, donations and contributions in cash or in other forms from the Government of India, State Governments, Charitable Institutions/Trusts, individuals and industry within the country; xii. To receive, with the prior approval of the Central Government, monetary assistance from foreign sources including international organizations for training programmes, scientific research and other activities; xiii. To acquire by gift, purchase, exchange, lease, hire or otherwise howsoever, any property movable or immovable or to construct, improve, alter, demolish or repair buildings and structures as may be necessary or convenient for carrying on the activities of the CENTRE; 7 xiv. For the purpose of the CENTRE, to draw and accept, make and endorse, discount and negotiate Government of India and other Promissory Notes, Bills of Exchange, Cheques or other Negotiable Instruments; xv. For investing the funds of or money entrusted to the CENTRE, to open such securities or in such manner as may from time to time be determined by the Governing Council and to sell or transpose such investment; xvi. To do all such other lawful acts as may be necessary, incidental or conducive to the attainment of all or any of the above objectives; xvii. To institute Professorships, other faculty positions, fellowships including visiting fellowships, research and cadre positions, scholarships, etc. for realizing the objectives of the CENTRE; xviii.To establish, maintain and manage laboratories, workshops, stores, library, office and other facilities for scientific and technological work of the CENTRE; xix. To acquire or transfer technical know-how from/to entrepreneurs and industries; and xx. To register patents, designs & technical know-how that may be developed by the CENTRE and transfer any portion of such patents/designs/technical know-how in the interest of the CENTRE. 8 From the Director’s Desk 9 10 Director’s Message It is my pleasure to present the Annual Report of CDFD for the year ending 31 March, 2010. Established in 1996, this Centre has emerged as an institute of national and international renown that provides services and training in the areas of DNA profiling and diagnostic testing for genetic disorders, and also undertakes cutting edge basic research in diverse areas of molecular biology. With the induction of new groups, and an increasing awareness about CDFD’s societal contributions, the Centre has been witnessing significant growth in all phases of activities, and several new frontiers of investigation have also been initiated. A perusal of the Annual Report would, I trust, bring out the Centre’s achievements in the current reporting year, and I would like to highlight a few of them below. OnIn the behalf area of of all DNA of my profiling colleagues services, in the this Centre, year, I CDFDam pleased was forwarded to enclose almost the Annual a hundred Report cases of CDFD by thefor thejudiciary year andending law enforcing31 March, agencies 2009. This of the has Union been and an differenteventful State year Governments.for the Centre CDFD both in also terms undertook of our achievementscapacity building in researchand training and on services DNA profiling and in viewfor the of statethe shifting forensic of scienceour activities laboratories to new interimof Uttar premises Pradesh inand Nampally Karnataka. in the The heart Medical of the Genetics city of Hyderabad. Unit, which was set up in August 2009 and is operated jointly with Initiallythe Nizam’s conceived Institute in 1996 of Medical as an institution Sciences, for Hyderabad,providing DNA has profiling been runningservices effectively.in medicolegal This cases year andthe diagnosticDiagnostics tests division for children had provided and families genetic with evaluation genetic and disorders, counselling the Centre to approximately has expanded 1800 its patients. activities sinceThis year, 1999 the to includeAPEDA-CDFD in its ambit Centre additional for Basmati services DNA such Analysis as DNA tested marker-based around 350 certification basmati samples of basmati for ricepurity. purity The andCDFD testing is also for a referralcertain centregenetically for genetic modified fidelity organisms testing of (GMOs), tissue culture as also raised more plants, importantly and in basicthis reporting research year, in diverse two new areas whole of genome modern scanning biology. The molecular combination markers of [viz. high-calibre inter-retrotransposon services delivery amplified and ofpolymorphism research at (IRAP)the Centre and retrotransposonhas emerged as microsatellite a unique model amplified that polymorphismstrives for academic (REMAP)] excellence were assessed tied to socialfor their relevance, utility in true-to-type in which each testing. of the two sets of activities is enriched by, and in turn enriches, the other. TheIn its major efforts activities to apply and RNAi achievements technology inof the controlCentre inof thisbaculovirus reporting infection year have of beensilkmoths, detailed the in Laboratory the pages whichof Molecular follow, Geneticsand the highlights has been alone successful are listed in transferring here. DNA theprofiling transgene services expressing and training dsRNA being for offered essential by theviral CDFD genes continue into the commercialto be sought cultivar after by CSR2, various which State is and a high Central yielding Government bivoltine strainagencies that andis extremely to enjoy highsusceptible credibility. to baculovirous Based on an infection. understanding In continued with the work Government on the gloverins, of Andhra which Pradesh, are a family it has of been antimicrobial decided thatpeptides the Centre encoded will in undertake the Bombyx DNA genome, profiling the free group of cost has for shown cases that referred loss of toan it intron by the from Andhra the ancestral Pradesh Stategene leadsGovernment to gain ofagencies. function in In overexpression the area of Diagnostic during embryonic services, stage.the highlight this year has been the establishment of a Medical Genetics Unit at the Nizam’s Institute of Medical Sciences (NIMS), Hyderabad, The Laboratory for Plant-Microbe Interactions has been engaged in studying interaction of different as part of a Memorandum of Understanding between CDFD and NIMS. The Medical Genetics Unit is Xanthomonas pathogens with their cognate host plants. The group has made biosensors that can detect already functional for
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