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Alcohol Dysregulates Mir-148A in Hepatocytes Through Foxo1

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Alcohol Dysregulates Mir-148A in Hepatocytes Through Foxo1 Hepatology ORIGINAL ARTICLE Gut: first published as 10.1136/gutjnl-2017-315123 on 23 February 2018. Downloaded from Alcohol dysregulates miR-148a in hepatocytes through FoxO1, facilitating pyroptosis via TXNIP overexpression Mi Jeong Heo,1 Tae Hyun Kim,1 Jueng Soo You,2 Delia Blaya,3 Pau Sancho-Bru,3 Sang Geon Kim1 ► Additional material is ABSTRact published online only. To view, Objective Alcoholic liver disease (ALD) is a leading Significance of this study please visit the journal online cause of death among chronic liver diseases. However, (http:// dx. doi. org/ 10. 1136/ What is already known on this subject? gutjnl- 2017- 315123). its pathogenesis has not been completely established. MicroRNAs (miRNAs) are key contributors to liver ► Excessive alcohol consumption promotes 1College of Pharmacy diseases progression. This study investigated hepatocyte- hepatocyte dysfunction and death during the and Research Institute of progression of liver diseases. Pharmaceutical Sciences, Seoul abundant miRNAs dysregulated by ALD, its impact on National University, Seoul, hepatocyte injury and the underlying basis. ► A set of microRNAs (miRNAs) including miR- Republic of Korea 182, miR-155 and miR-217 showed abnormal 2 Design Alcoholic hepatitis (AH) human and animal Department of Biochemistry, liver samples and hepatocytes were used to assess increases in alcoholic liver disease (ALD). School of Medicine, Konkuk However, the downregulated miRNAs have University, Seoul, Republic of miR-148a levels. Pre-miR-148a was delivered Korea specifically to hepatocytes in vivo using lentivirus. been scarcely explored. 3Laboratory of Liver Cell Immunoblottings, luciferase reporter assays, chromatin ► MiR-148a belongs to miRNAs abundant Plasticity and Tissue Repair, immunoprecipitation and immunofluorescence assays in hepatocytes and regulates hepatocyte Institut d’Investigacions were carried out in cell models. differentiation. MIR148A gene is regulated by Biomèdiques August Pi i the transcription factors, SREBP-1, MYB and Sunyer (IDIBAPS), Centro de Results The miRNA profile and PCR analyses enabled Investigación Biomédica en Red us to find substantial decrease of miR-148a in the liver p53. de Enfermedades Hepáticasy of patients with AH. In mice subjected to Lieber-DeCarli ► Thioredoxin-interacting protein (TXNIP) Digestivas (CIBERehd), alcohol diet or binge alcohol drinking, miR-148a levels in association with NLRP3 activates Barcelona, Spain were also markedly reduced. In cultured hepatocytes and inflammasome pathway during the progression of non alcoholic fatty liver disease . http://gut.bmj.com/ Correspondence to mouse livers, alcohol exposure inhibited forkhead box Dr. Sang Geon Kim, College protein O1 (FoxO1) expression, which correlated with of Pharmacy, Seoul National miR-148a levels and significantly decreased in humanA H What are the new findings? University, 1Gwanakro, Seoul specimens. FoxO1 was identified as a transcription factor ► MiR-148a levels are dysregulated in the liver 08826, Republic of Korea; sgk@ for MIR148A transactivation. MiR-148a directly inhibited of patients with alcoholic hepatitis (AH) or snu. ac. kr thioredoxin-interacting protein (TXNIP) expression. ethanol-fed animals. A part of the data included Consequently, treatment of hepatocytes with ethanol ► Forkhead box protein O1 is identified as an in this manuscript was resulted in TXNIP overexpression, activating NLRP3 unrecognised transcriptional regulator of on September 30, 2021 by guest. Protected copyright. presented at the 2017 Spring inflammasome and caspase-1-mediated pyroptosis. MIR148A gene, and its hepatic expression International Convention of the These events were reversed by miR-148a mimic or TXNIP is diminished in patients with AH or animal Pharmaceutical Society of Korea models, lowering miR-148a levels. in Korea (2017). small-interfering RNA transfection. Hepatocyte-specific delivery of miR-148a to mice abrogated alcohol-induced ► TXNIP is discovered as a direct target of Received 23 August 2017 TXNIP overexpression and inflammasome activation, miR-148a and is overexpressed during the Revised 29 January 2018 attenuating liver injury. progression of ALD . Accepted 31 January 2018 Conclusion Alcohol decreases miR-148a expression ► Alcohol treatment elicits caspase-1-mediated in hepatocytes through FoxO1, facilitating TXNIP pyroptosis through TXNIP overexpression, overexpression and NLRP3 inflammasome activation, and this event is reversed by miR-148a which induces hepatocyte pyroptosis. Our findings transfection. Consistently, hepatocyte-specific provide information on novel targets for reducing delivery of miR-148a alleviates alcoholic liver incidence and progression of ALD. injury. How might it impact on clinical practice in the foreseeable future? INTRODUCTION ► Our findings demonstrate that miR-148a Excessive alcohol drinking promotes alcoholic liver ameliorates alcohol-induced inflammasome disease (ALD) such as steatosis, steatohepatitis, To cite: Heo MJ, Kim TH, activation and pyroptosis in hepatocytes You JS, et al. Gut Epub ahead fibrosis to cirrhosis and finally to hepatocellular through TXNIP inhibition, which shed light on of print: [please include Day carcinoma, the leading causes of death among all novel targets and potential strategies for the Month Year]. doi:10.1136/ chronic liver diseases. Alcoholic hepatitis (AH) with treatment of ALD. gutjnl-2017-315123 liver cirrhosis accounts for approximately 50% of Heo MJ, et al. Gut 2018;0:1–13. doi:10.1136/gutjnl-2017-315123 1 Hepatology the mortality in Western countries.1 Because of the rapid increase calories) ad libitum or an isocaloric liquid diet for 4–6 weeks. For in alcohol consumption in other countries such as China and binge alcohol model, ethanol (5 g/kg body weight) was adminis- Gut: first published as 10.1136/gutjnl-2017-315123 on 23 February 2018. Downloaded from India, the prevalence of ALD has increased globally.2 However, tered two times daily by gastric intubation for 7 consecutive days, successful therapeutic remedies are not available presumably whereas control mice received water. For in vivo miRNA delivery because of inadequate patient characterisation, poor diagnostic experiment, mice were injected with lentiviruses expressing control as well as prognostic measures and lack of proper medication. or pre-miR-148a through tail vein. An understanding of the pathogenesis of ALD and the molec- ular regulation may assist in the development of good diagnostic Chromatin immunoprecipitation assay markers, along with preventive and/or therapeutic approaches. The chromatin immunoprecipitation (ChIP) assay was performed The microRNAs (miRNAs) participate in a variety of patho- according to EZ-ChIP assay kit protocol (Upstate Biotechnology, physiological processes. A set of miRNAs including miR-182, Lake Placid, New York, USA). The procedures for analysis are miR-155 and miR-217 showed an abnormal increase in ALD.3–5 described in online supplementary materials and methods. More importantly, decrease of miRNA and the resultant over- expression of its target may also be crucial in understanding the process of hepatocyte death and disease progression. However, Flow cytometric analysis the significance of downregulated miRNAs has been scarcely Pyroptosis was analysed using the FAM-FLICA in vitro Caspase-1 explored in ALD (except the dysregulation of miR-122).6 This Detection Kit (ImmunoChemistry Technologie, Bloomington, study aims at identifying a liver-specific miRNA, which is down- Minnesota, USA). The procedures for analysis are described in regulated during the progression of ALD along with its target online supplementary materials and methods. and investigates their roles in determining hepatocyte fate and the underlying molecular basis. Data analysis Alcohol consumption and detoxification processes have Statistically significant differences were assessed by the Student’s direct effects on hepatocytes, such as release of sterile danger t-test or one-way analysis of variance tests followed by Bonfer- signals, uric acid and extracellular ATP.7 The signals released roni’s method or Fisher's Least Significant Difference for multiple from damaged hepatocytes activate multiple inflammatory path- comparisons when necessary. The data were expressed as the ways, promoting ALD.8 In addition, patients with AH showed mean±SEM. Coefficients of correlation (r) were determined increased levels of tumour necrosis alpha and interleukin 1 by the Pearson correlation method. The criterion for statistical (IL-1),9 10 supporting the hypothesis that inflammatory response significance was set at P<0.05 or P<0.01. is the major driving force during ALD progression. Moreover, Additional detailed information is in online supplementary alcohol metabolism in hepatocytes facilitates production of reac- materials and methods. tive oxygen species (ROS) and mitochondrial dysfunction, sensi- tising hepatocytes to inflammatory cytokines.11 12 In addition to the immune cell-mediated inflammation, inflammasome-depen- RESULTS dent IL-1β production has also been reported in hepatocytes.13 Dysregulation of miR-148a in patients with AH or ALD animal Nonetheless, whether alcohol consumption promotes inflam- models http://gut.bmj.com/ masome activation in hepatocytes, and if so, what the conse- To determine the identity of the miRNAs dysregulated in patients quence is for hepatocyte fate had not been explored. with AH, we first analysed the profile of miRNA microarray Here, we report that miR-148a is markedly decreased
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