DOCSLIB.ORG

Enrofloxacin Shifts Intestinal Microbiota and Metabolic Profiling and Hinders Recovery

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Enrofloxacin Shifts Intestinal Microbiota and Metabolic Profiling and Hinders Recovery bioRxiv preprint doi: https://doi.org/10.1101/2020.07.13.201830; this version posted July 14, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Enrofloxacin shifts intestinal microbiota and metabolic profiling and hinders recovery 2 from Salmonella enterica subsp. enterica serovar Typhimurium infection in neonatal 3 chickens 4 5 Boheng Ma,a,b,c,& Xueran Mei,a,b,c,& Changwei Lei,a,b,c Cui Li,a,b,c Yufeng Gao,a,b,c Linghan 6 Kong,a,b,c Xiwen Zhai,a,b,c,d Hongning Wanga,b,c,# 7 8 a College of Life Sciences, Sichuan University, Chengdu, People’s Republic of China 9 b Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Chengdu, 10 People’s Republic of China 11 c Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, 12 People’s Republic of China 13 d Sichuan Water Conservancy Vocational Collage, Chengdu, People’s Republic of China 14 15 Running head: Effects of enrofloxacin on chickens 16 17 # Address correspondence to Hongning Wang, [email protected] 18 & Both authors are identified as Co-First Author. 19 20 Word count: 211 words (Abstract); 4895 words (main text) 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.07.13.201830; this version posted July 14, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 21 ABSTRACT 22 Enrofloxacin is an important antibiotic used for prevention and treatment of Salmonella infection 23 in poultry in many countries. However, oral administration of enrofloxacin may lead to the 24 alterations in the microbiota and metabolome in the chicks intestine, thereby reducing 25 colonization resistance to the Salmonella infection. To study the effects of enrofloxacin on 26 chicken cecal Salmonella, we used different concentrations of enrofloxacin to feed 1-day-old 27 chickens, followed by oral challenge with Salmonella enterica subsp. enterica serovar 28 Typhimurium (S. Typhimurium). We then explored the distribution patterns of S. Typhimurium in 29 vivo in intestinal contents using quantitative polymerase chain reaction and microbial 16S 30 amplicon sequencing on days 7, 14, and 21. Metabolome sequencing was used to explore the gut 31 metabolome on day 14. Faecalibacterium and Anaerostipes, which are closely related to the 32 chicken intestinal metabolome, were screened using a multi-omics technique. The abundance of 33 S. Typhimurium was significantly higher in the enrofloxacin-treated group than in the untreated 34 group, and S. Typhimurium persisted longer. Moreover, the cecal colony structures of the three 35 groups exhibited different characteristics, with Lactobacillus reaching its highest abundance on 36 day 21. Notably, S. Typhimurium infection is known to affect the fecal metabolome of chickens 37 differently. Thus, our results suggested that enrofloxacin and Salmonella infections completely 38 altered the intestinal metabolism of chickens. 39 40 KEYWORDS gut microbiota, enrofloxacin, metabolome, chicken, Salmonella Typhimurium 41 2 bioRxiv preprint doi: https://doi.org/10.1101/2020.07.13.201830; this version posted July 14, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 42 IMPORTANCE 43 In this study, we examined the effects of S. Typhimurium infection and enrofloxacin treatment on 44 the microbial flora and metabolite synthesis in chicken guts in order to identify target metabolites 45 that may cause S. Typhimurium colonization and severe inflammation and to evaluate the 46 important flora that may be associated with these metabolites. Our findings may facilitate the use 47 of antibiotics to prevent S. Typhimurium infection. 3 bioRxiv preprint doi: https://doi.org/10.1101/2020.07.13.201830; this version posted July 14, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 48 INTRODUCTION 49 Foodborne diseases are associated with high morbidity and mortality rates worldwide and pose 50 major challenges to food safety and economics (1). Salmonella is an important foodborne 51 pathogen of the family Enterobacteriaceae. This pathogen causes millions of infections 52 worldwide each year, both in humans and livestock. Salmonella has been shown to cause 53 intestinal inflammation and barrier dysfunction in chickens, thereby affecting chicken 54 performance (2). Currently, more than 2,600 serotypes of Salmonella are known, with varying 55 pathogenicity and host specificity. Salmonella enterica subsp. enterica serovar Typhimurium (S. 56 Typhimurium) is a typical representative of non-host-specific Salmonella found in poultry. 57 The main route of infection with Salmonella in poultry is the fecal-oral route. For 58 multiserotype infections, bacteria mainly colonize in the ceca of poultry. After colonizing the 59 intestines, these bacteria invade the intestinal epithelial cells and dendritic cells and reach the 60 submucosa to be phagocytosed by macrophages. The bacteria then replicate in and colonize the 61 spleen and liver, causing systemic infections mediated by bacterium/macrophage interactions (3, 62 4). In mice infected with Salmonella, macrophages produce IL-12 and IL-18 and promote IFN-γ 63 secretion by natural killer (NK) cells (5). 64 Owing to the widespread use of antibiotics in agriculture and animal husbandry, 65 Salmonella resistance has rapidly increased. In 2013, China alone consumed approximately 66 927,000 tons of antibiotics (6). Moreover, the consumption of antibiotics as veterinary drugs 67 increased from 46% to 52% between 2007 and 2013. Enrofloxacin, florfenicol, lincomycin, and 68 amoxicillin are among the most commonly consumed veterinary antibiotics in China (7). 69 Enrofloxacin is the main antibiotic used for treating Salmonella infection in poultry in many 4 bioRxiv preprint doi: https://doi.org/10.1101/2020.07.13.201830; this version posted July 14, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 70 countries, and the concentration of enrofloxacin in chicken manure in China was found to be 71 1,421 mg/kg (8). 72 Quinolones, such as enrofloxacin, affect the composition and number of bacterial species 73 in the chicken gut (9), thereby altering resistance to pathogen invasion. Indeed, bacteria residing 74 in the gut can mediate the colonization of S. Typhimurium in the body by producing short-chain 75 fatty acids (SCFAs), such as propionic acid, which inhibits S. Typhimurium growth by 76 influencing the intracellular pH (10). By producing SCFAs, lactic acid bacteria inhibit 77 Salmonella colonization in the colon and jejunum, accelerate Salmonella clearance from stools, 78 and reduce Salmonella transfer from the small intestine to the spleen (11). The concentrations of 79 SCFAs and butyric acid are inversely proportional to the degree of inflammation (12). 80 Some Enterobacteriaceae in the normal gut flora can mediate Salmonella multiplication 81 through competition for oxygen (13). Additionally, antibiotics reduce the number of butyrate- 82 producing Clostridium perfringens in the intestinal flora, shifting the host cells from oxidative 83 metabolism to lactic acid fermentation and increasing the level of lactic acid in the intestine; 84 Salmonella then gain a colonization advantage by using lactic acid (14). Intestinal metabolism is 85 also affected by the gut flora composition. Using antibiotics increases fucose and sialic acid 86 contents in the intestine, thereby affecting Salmonella and C. difficile colonization (15, 16). 87 Similarly, antibiotics also decrease the levels of secondary bile acids in the intestine and inhibit 88 C. difficile colonization (16). 89 Accordingly, in this study, we investigated the effects of enrofloxacin on Salmonella 90 colonization in chickens and the relationship between intestinal flora and Salmonella 91 colonization. We also evaluated changes in microbial metabolites in the ceca of enrofloxacin- 5 bioRxiv preprint doi: https://doi.org/10.1101/2020.07.13.201830; this version posted July 14, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 92 treated chickens infected with Salmonella and used multiomics association analyses to determine 93 the correlations between microbial community structure and microbial metabolites. 94 6 bioRxiv preprint doi: https://doi.org/10.1101/2020.07.13.201830; this version posted July 14, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 95 RESULTS 96 Response of Salmonella to enrofloxacin 97 First, S. Typhimurium abundance in the samples was evaluated. S. Typhimurium was not isolated 98 from C1, C2, or C3 groups. On day 7, the highest abundance of S. Typhimurium was found in the 99 cecum contents of group E1 (6.128 log10CFU/g), followed by that of groups E3 and (5.090 and 100 3.945 log10CFU/g, respectively; P < 0.001). The abundances of S. Typhimurium in the heart, 101 liver, and spleen of group E1 were significantly higher than those in the other two groups (P < 102 0.001). On day 14, the abundance of S. Typhimurium in group E1 (3.371 log10CFU/g) was 103 significantly lower than those in groups E2 (4.829 log10CFU/g) and E3 (4.366 log10CFU/g; P < 104 0.01). Moreover, the abundances of S. Typhimurium were significantly lower in heart, liver, and 105 spleen samples from group E1 than those from groups E2 and E3, except for heart samples from 106 group E3. On day 21, the relative abundances of S. Typhimurium were 3.170, 5.146, and 5.636 107 log10CFU/g in groups E1, E2, and E3, respectively; that in group E1 was significantly lower than 108 those in the other two groups.
Load more

Recommended publications
  • Supplementary Information for Acute Infection of Mice with Clostridium
    IMM-2013-2862, Sadighi Akha et al-Supplementary Information 1 Supplementary Information for Acute infection of mice with Clostridium difficile leads to eIF2α phosphorylation and pro-survival signalling as part of the mucosal inflammatory response Amir A. Sadighi Akha1, Casey M. Theriot2, John R. Erb-Downward1, Andrew J. McDermott3, Nicole R. Falkowski1, Heather M. Tyra4, D. Thomas Rutkowski4, Vincent B. Young2,3 and Gary B. Huffnagle1,3* IMM-2013-2862, Sadighi Akha et al-Supplementary Information 2 Supplementary Text Materials and Methods for Figures S1 and S2 DNA isolation, amplicon library preparation and 454 pyrosequencing- The DNeasy blood and tissue kit (Qiagen) was used to extract genomic DNA from the caecal and colonic contents obtained from untreated and C. difficile-infected mice. The extraction was performed according to the manufacturer’s instructions except for the following modifications: adding a bead-beating step using UltraClean fecal DNA bead tubes (Mo Bio Laboratories, Carlsbad, CA); doubling the amount of ATL buffer and the proteinase K used in the protocol; and decreasing by half the amount of the AE buffer used to elute the DNA. Subsequently, the V3, V4 and V5 hyper-variable regions of the 16S ribosomal RNA gene in each of the samples were targeted for amplification with the 357F and 929R primer sets [1]. Amplicons were purified with the Agencourt AMPure XP PCR purification system (Beckman Coulter, Indianapolis, IN), and quantified with the Quant- iT PicoGreen dsDNA kit (Life Technologies) to obtain an equal pool for pyrosequencing. They were then sequenced on a Roche 454 GS Junior Titanium platform according to the manufacturer’s specifications.
    [Show full text]
  • Fatty Acid Diets: Regulation of Gut Microbiota Composition and Obesity and Its Related Metabolic Dysbiosis
    International Journal of Molecular Sciences Review Fatty Acid Diets: Regulation of Gut Microbiota Composition and Obesity and Its Related Metabolic Dysbiosis David Johane Machate 1, Priscila Silva Figueiredo 2 , Gabriela Marcelino 2 , Rita de Cássia Avellaneda Guimarães 2,*, Priscila Aiko Hiane 2 , Danielle Bogo 2, Verônica Assalin Zorgetto Pinheiro 2, Lincoln Carlos Silva de Oliveira 3 and Arnildo Pott 1 1 Graduate Program in Biotechnology and Biodiversity in the Central-West Region of Brazil, Federal University of Mato Grosso do Sul, Campo Grande 79079-900, Brazil; [email protected] (D.J.M.); [email protected] (A.P.) 2 Graduate Program in Health and Development in the Central-West Region of Brazil, Federal University of Mato Grosso do Sul, Campo Grande 79079-900, Brazil; pri.fi[email protected] (P.S.F.); [email protected] (G.M.); [email protected] (P.A.H.); [email protected] (D.B.); [email protected] (V.A.Z.P.) 3 Chemistry Institute, Federal University of Mato Grosso do Sul, Campo Grande 79079-900, Brazil; [email protected] * Correspondence: [email protected]; Tel.: +55-67-3345-7416 Received: 9 March 2020; Accepted: 27 March 2020; Published: 8 June 2020 Abstract: Long-term high-fat dietary intake plays a crucial role in the composition of gut microbiota in animal models and human subjects, which affect directly short-chain fatty acid (SCFA) production and host health. This review aims to highlight the interplay of fatty acid (FA) intake and gut microbiota composition and its interaction with hosts in health promotion and obesity prevention and its related metabolic dysbiosis.
    [Show full text]
  • Environment-Dependent Variation in Gut Microbiota of an Oviparous Lizard (Calotes Versicolor)
    animals Article Environment-Dependent Variation in Gut Microbiota of an Oviparous Lizard (Calotes versicolor) Lin Zhang 1,2,*,† , Fang Yang 3,†, Ning Li 4 and Buddhi Dayananda 5 1 School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China 2 State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao 266237, China 3 School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan 430065, China; [email protected] 4 College of Food Science, Nanjing Xiaozhuang University, Nanjing 211171, China; [email protected] 5 School of Agriculture and Food Sciences, The University of Queensland, Brisbane, QLD 4072, Australia; [email protected] * Correspondence: [email protected] † These authors contributed equally to this work. Simple Summary: The different gut sections potentially provide different habitats for gut microbiota. We found that Bacteroidetes, Firmicutes, and Proteobacteria were the three primary phyla in gut micro- biota of C. versicolor. The relative abundance of dominant phyla Bacteroidetes and Firmicutes exhibited an increasing trend from the small intestine to the large intestine, and there was a higher abun- dance of genus Bacteroides (Class: Bacteroidia), Coprobacillus and Eubacterium (Class: Erysipelotrichia), Parabacteroides (Family: Porphyromonadaceae) and Ruminococcus (Family: Lachnospiraceae), and Family Odoribacteraceae and Rikenellaceae in the hindgut, and some metabolic pathways were higher in the hindgut. Our results reveal the variations of gut microbiota composition and metabolic pathways in Citation: Zhang, L.; Yang, F.; Li, N.; different parts of the lizards’ intestine. Dayananda, B. Environment- Dependent Variation in Gut Abstract: Vertebrates maintain complex symbiotic relationships with microbiota living within their Microbiota of an Oviparous Lizard gastrointestinal tracts which reflects the ecological and evolutionary relationship between hosts and (Calotes versicolor).
    [Show full text]
  • The Gut Microbiota Profile According to Glycemic Control in Type 1
    microorganisms Article The Gut Microbiota Profile According to Glycemic Control in Type 1 Diabetes Patients Treated with Personal Insulin Pumps Sandra Mrozinska 1,2 , Przemysław Kapusta 3 , Tomasz Gosiewski 4 , Agnieszka Sroka-Oleksiak 4, Agnieszka H. Ludwig-Słomczy ´nska 3 , Bartłomiej Matejko 1,2 , Beata Kiec-Wilk 1,2, Malgorzata Bulanda 4, Maciej T. Malecki 1,2, Pawel P. Wolkow 3 and Tomasz Klupa 1,2,* 1 Jagiellonian University Medical College, Faculty of Medicine, Department of Metabolic Diseases, 2 Jakubowskiego Street, 30-688 Krakow, Poland; [email protected] (S.M.); [email protected] (B.M.); [email protected] (B.K.-W.); [email protected] (M.T.M.) 2 University Hospital, Department of Metabolic Diseases, 2 Jakubowskiego Street, 30-688 Krakow, Poland 3 Jagiellonian University Medical College, Center for Medical Genomics OMICRON, 7c Kopernika Street, 31-034 Krakow, Poland; [email protected] (P.K.); [email protected] (A.H.L.-S.); [email protected] (P.P.W.) 4 Jagiellonian University Medical College, Faculty of Medicine, Department of Microbiology, 18 Czysta Street, 31-121 Krakow, Poland; [email protected] (T.G.); [email protected] (A.S.-O.); [email protected] (M.B.) * Correspondence: [email protected]; Tel.: +48-1240-029-50 Abstract: Recently, several studies explored associations between type 1 diabetes (T1DM) and micro- biota. The aim of our study was to assess the colonic microbiota structure according to the metabolic control in T1DM patients treated with insulin pumps. We studied 89 T1DM patients (50.6% women) at the median age of 25 (IQR, 22–29) years.
    [Show full text]
  • Taxonomy of Lactobacilli and Bifidobacteria
    Curr. Issues Intestinal Microbiol. 8: 44–61. Online journal at www.ciim.net Taxonomy of Lactobacilli and Bifdobacteria Giovanna E. Felis and Franco Dellaglio*† of carbohydrates. The genus Bifdobacterium, even Dipartimento Scientifco e Tecnologico, Facoltà di Scienze if traditionally listed among LAB, is only poorly MM. FF. NN., Università degli Studi di Verona, Strada le phylogenetically related to genuine LAB and its species Grazie 15, 37134 Verona, Italy use a metabolic pathway for the degradation of hexoses different from those described for ‘genuine’ LAB. Abstract The interest in what lactobacilli and bifdobacteria are Genera Lactobacillus and Bifdobacterium include a able to do must consider the investigation of who they large number of species and strains exhibiting important are. properties in an applied context, especially in the area of Before reviewing the taxonomy of those two food and probiotics. An updated list of species belonging genera, some basic terms and concepts and preliminary to those two genera, their phylogenetic relationships and considerations concerning bacterial systematics need to other relevant taxonomic information are reviewed in this be introduced: they are required for readers who are not paper. familiar with taxonomy to gain a deep understanding of The conventional nature of taxonomy is explained the diffculties in obtaining a clear taxonomic scheme for and some basic concepts and terms will be presented for the bacteria under analysis. readers not familiar with this important and fast-evolving area,
    [Show full text]
  • Downloaded from the VFDB Data- Base (Virulence Factors Database) Containing Nucleotide Sample Collection and Metagenomic Sequencing Sequences of 2585 Genes [53]
    Dubinkina et al. Microbiome (2017) 5:141 DOI 10.1186/s40168-017-0359-2 RESEARCH Open Access Links of gut microbiota composition with alcohol dependence syndrome and alcoholic liver disease Veronika B. Dubinkina1,2,3,4, Alexander V. Tyakht2,5*, Vera Y. Odintsova2, Konstantin S. Yarygin1,2, Boris A. Kovarsky2, Alexander V. Pavlenko1,2, Dmitry S. Ischenko1,2, Anna S. Popenko2, Dmitry G. Alexeev1,2, Anastasiya Y. Taraskina6, Regina F. Nasyrova6, Evgeny M. Krupitsky6, Nino V. Shalikiani7, Igor G. Bakulin7, Petr L. Shcherbakov7, Lyubov O. Skorodumova2, Andrei K. Larin2, Elena S. Kostryukova1,2, Rustam A. Abdulkhakov8, Sayar R. Abdulkhakov8,9, Sergey Y. Malanin9, Ruzilya K. Ismagilova9, Tatiana V. Grigoryeva9, Elena N. Ilina2 and Vadim M. Govorun1,2 Abstract Background: Alcohol abuse has deleterious effects on human health by disrupting the functions of many organs and systems. Gut microbiota has been implicated in the pathogenesis of alcohol-related liver diseases, with its composition manifesting expressed dysbiosis in patients suffering from alcoholic dependence. Due to its inherent plasticity, gut microbiota is an important target for prevention and treatment of these diseases. Identification of the impact of alcohol abuse with associated psychiatric symptoms on the gut community structure is confounded by the liver dysfunction. In order to differentiate the effects of these two factors, we conducted a comparative “shotgun” metagenomic survey of 99 patients with the alcohol dependence syndrome represented by two cohorts—with and without liver cirrhosis. The taxonomic and functional composition of the gut microbiota was subjected to a multifactor analysis including comparison with the external control group. Results: Alcoholic dependence and liver cirrhosis were associated with profound shifts in gut community structures and metabolic potential across the patients.
    [Show full text]
  • Draft Genome and Description of Merdibacter Massiliensis Gen.Nov., Sp
    www.nature.com/scientificreports OPEN Draft genome and description of Merdibacter massiliensis gen.nov., sp. nov., a new bacterium genus Received: 21 November 2018 Accepted: 9 May 2019 isolated from the human ileum Published: xx xx xxxx Hussein Anani1, Rita Abou Abdallah1, Nisrine Chelkha2, Anthony Fontanini2, Davide Ricaboni2, Morgane Mailhe2, Didier Raoult2,3 & Pierre-Edouard Fournier 1 We used phenotypic, genomic and phylogenetic information following the taxono-genomics approach to demonstrate that strain Marseille–P3254, isolated from an ileal sample of a 76-year old woman who underwent upper and lower digestive tract endoscopy for esophagitis and colonic polyp, is representative of a novel bacterial genus within the family Erysipelotrichaceae in the phylum Firmicutes. It is an anaerobic Gram-negative bacterium without catalase and oxidase activities. The genome of strain Marseille–P3254 is 2,468,496-bp long with a 40.1% G + C content. This new bacterium is most closely related to Eubacterium dolichum, with which it shares 90.7% 16S rRNA sequence similarity. In addition, genomic comparison using the digital DNA–DNA hybridization and OrthoANI analyses between the novel organism and the E. dolichum type strain revealed identities of 25.2 and 68.91%, respectively. The major fatty acids were C16: 0, C18: 1n9 and C18: 0. Based on these data, we propose the creation of the new genus Merdibacter gen. nov., with strain Marseille-P3254T (=CSUR P3254 = DSM 103534) being the type strain of the new species Merdibacter massiliensis gen. nov., sp. nov. A thorough knowledge of the gut microbiota composition appears essential to understand many aspects of health and diseases in humans.
    [Show full text]
  • Metagenome-Wide Association of Gut Microbiome Features for Schizophrenia
    ARTICLE https://doi.org/10.1038/s41467-020-15457-9 OPEN Metagenome-wide association of gut microbiome features for schizophrenia Feng Zhu 1,19,20, Yanmei Ju2,3,4,5,19,20, Wei Wang6,7,8,19,20, Qi Wang 2,5,19,20, Ruijin Guo2,3,4,9,19,20, Qingyan Ma6,7,8, Qiang Sun2,10, Yajuan Fan6,7,8, Yuying Xie11, Zai Yang6,7,8, Zhuye Jie2,3,4, Binbin Zhao6,7,8, Liang Xiao 2,3,12, Lin Yang6,7,8, Tao Zhang 2,3,13, Junqin Feng6,7,8, Liyang Guo6,7,8, Xiaoyan He6,7,8, Yunchun Chen6,7,8, Ce Chen6,7,8, Chengge Gao6,7,8, Xun Xu 2,3, Huanming Yang2,14, Jian Wang2,14, ✉ ✉ Yonghui Dang15, Lise Madsen2,16,17, Susanne Brix 2,18, Karsten Kristiansen 2,17,20 , Huijue Jia 2,3,4,9,20 & ✉ Xiancang Ma 6,7,8,20 1234567890():,; Evidence is mounting that the gut-brain axis plays an important role in mental diseases fueling mechanistic investigations to provide a basis for future targeted interventions. However, shotgun metagenomic data from treatment-naïve patients are scarce hampering compre- hensive analyses of the complex interaction between the gut microbiota and the brain. Here we explore the fecal microbiome based on 90 medication-free schizophrenia patients and 81 controls and identify a microbial species classifier distinguishing patients from controls with an area under the receiver operating characteristic curve (AUC) of 0.896, and replicate the microbiome-based disease classifier in 45 patients and 45 controls (AUC = 0.765). Functional potentials associated with schizophrenia include differences in short-chain fatty acids synth- esis, tryptophan metabolism, and synthesis/degradation of neurotransmitters.
    [Show full text]
  • GA-Map Dysbiosis Test Sample Report 1
    Genetic Analysis AS Kabelgata 8 0580 Oslo www.genetic-analysis.com Postboks 243 Økern 0510 Oslo, Norway GA-map® Dysbiosis Test Lx – Test Results SAMPLE ID: Collection Date: Date Received: Report Date: DYSBIOSIS INDEX (DI) * The gut microbiota is 11 2 3 4 5 non-dysbiotic FUNCTIONAL IMBALANCE - BACTERIA PROFILES * FUNCTIONAL PROFILES TEST RESULTS COMMENTS A Butyrate producing bacteria Normal levels of major butyrate producers. B Gut mucosa protective bacteria Normal levels of important gut mucosa protective bacteria. Normal level of Faecalibacterium prausnitzii, a key bacterium that C Gut health marker promotes intestinal health. Gut barrier protective bacteria Balance between selected gut barrier protective and potentially D and potentially harmful bacteria harmful bacteria. E Pro-inflammatory bacteria Low levels of pro-inflammatory bacteria. ® Page * For a more detailed explanation of the results, please refer to pages 3-4, 'GA-map Dysbiosis Test Lx - REPORT FORM SUPPLEMENT'. 1/4 SAMPLE ID: Collection Date: Date Received: Report Date: BACTERIA ABUNDANCES * Normal ** Normal ** Reduced Elevated Reduced Elevated Actinobacteria -3 -2 -1 1 2 3 Firmicutes cont. -3 -2 -1 1 2 3 100 Actinobacteria 315 [Eubacterium] rectale 101 Actinomycetales 316 Eubacterium siraeum 103 Bifidobacterium spp. 317 Faecalibacterium prausnitzii Bacteriodetes 318 Lachnospiraceae 319 Lactobacillus ruminis & 201 Alistipes Pediococcus acidilactici 320 Lactobacillus spp. 202 Alistipes onderdonkii 321 Lactobacillus spp. 2 203 Bacteroides fragilis 322 Phascolarctobacterium sp. 204 Bacteroides pectinophilus 323 Ruminococcus albus & 205 Bacteroides spp. R. bromii Bacteroides spp. & 324 Ruminococcus gnavus 206 Prevotella spp. 325 Streptococcus agalactiae & 207 Bacteroides stercoris [Eubacterium] rectale Streptococcus salivarius ssp. Bacteroides 326 thermophilus & S. sanguinis 208 zoogleoformans 327 Streptococcus salivarius 209 Parabacteroides johnsonii ssp.
    [Show full text]
  • Cecum Microbial Communities from Steers Differing in Feed Efficiency1,2,3
    Published October 2, 2015 Cecum microbial communities from steers differing in feed efficiency1,2,3 P. R. Myer,*4 J. E. Wells,* T. P. L. Smith,* L. A. Kuehn,* and H. C. Freetly*5 *USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE 689336 ABSTRACT: Apart from the rumen, limited knowl- bial populations and operational taxonomic units edge exists regarding the structure and function of revealed significant differences among feed efficien- bacterial communities within the gastrointestinal tract cy groups (P < 0.05). Firmicutes was the dominant and their association with beef cattle feed efficiency. cecal phylum in all groups and accounted for up to The objective of this study was to characterize the 81% of the populations among samples. Populations microbial communities of the cecum among steers were also dominated by families Ruminococcaceae, differing in feed efficiency. Within 2 contemporary Lachnospiraceae, and Clostridiaceae, with sig- groups of steers, individual feed intake and BW gain nificant shifts in the relative abundance of taxa were determined from animals fed the same diet. among feed efficiency groups, including families Within both of 2 contemporary groups, BW was Ruminococcaceae (P = 0.040), Lachnospiraceae regressed on feed intake and 4 steers within each (P = 0.020), Erysipelotrichaceae (P = 0.046), and Cartesian quadrant were sampled (n = 16/group). Clostridiaceae (P = 0.043) and genera Coprobacillus Bacterial 16S rRNA gene amplicons were sequenced (P = 0.049), Parabacteroides (P = 0.044), Blautia (P = from the cecal content using next-generation sequenc- 0.042), Ruminococcus (P = 0.040), Oscillospira (P = ing technology. No significant changes in diversity or 0.042), and Prevotella (P = 0.042).
    [Show full text]
  • Relationships Between Diet-Related Changes in the Gut Microbiome and Cognitive Flexibility
    Relationships Between Diet-Related Changes in the Gut Microbiome and Cognitive Flexibility Magnusson, K. R., Hauck, L., Jeffrey, B. M., Elias, V., Humphrey, A., Nath, R., Perrone, A., & Bermudez, L. E. (2015). Relationships Between Diet-Related Changes in the Gut Microbiome and Cognitive Flexibility. [Article in Press]. Neuroscience. Elsevier Accepted Manuscript http://cdss.library.oregonstate.edu/sa-termsofuse *Manuscript (Clear Copy) Click here to view linked References 1 2 3 4 Title: RELATIONSHIPS BETWEEN DIET-RELATED CHANGES IN THE GUT MICROBIOME 5 AND COGNITIVE FLEXIBILITY 6 7 Authors: K.R. Magnusson 1, 2, L. Hauck1, B.M. Jeffrey1, V. Elias 1,2, A. Humphrey1, R. Nath3, A. 8 1, 2 1 9 Perrone , and L.E. Bermudez 10 11 Affiliations: 1 Dept Biomedical Sciences, College of Veterinary Medicine 12 2 Linus Pauling Institute 13 3 Dept of Human Development and Family Sciences, School of Social and 14 Behavioral Health Sciences 15 Oregon State University, Corvallis, OR 97331 U.S.A. 16 17 18 Email addresses: Magnusson: [email protected] 19 Hauck: [email protected] 20 Jeffrey: [email protected] 21 Elias: [email protected] 22 Humphrey: [email protected] 23 Nath: [email protected] 24 Perrone: [email protected] 25 Bermudez: [email protected] 26 27 28 Corresponding Author: Dr. Kathy Magnusson 29 307 Linus Pauling Science Center 30 Oregon State University 31 Corvallis, OR 97331 U.S.A. 32 Phone: 541-737-6923 33 Fax: 541-737-5077 34 Email: [email protected] 35 36 37 38 39 40 41
    [Show full text]
  • Fecal Metagenomic Profiles in Subgroups of Patients with Myalgic Encephalomyelitis/ Chronic Fatigue Syndrome Dorottya Nagy-Szakal1†, Brent L
    Nagy-Szakal et al. Microbiome (2017) 5:44 DOI 10.1186/s40168-017-0261-y RESEARCH Open Access Fecal metagenomic profiles in subgroups of patients with myalgic encephalomyelitis/ chronic fatigue syndrome Dorottya Nagy-Szakal1†, Brent L. Williams1†, Nischay Mishra1, Xiaoyu Che1, Bohyun Lee1, Lucinda Bateman2, Nancy G. Klimas3,9, Anthony L. Komaroff4, Susan Levine5, Jose G. Montoya6, Daniel L. Peterson7, Devi Ramanan8, Komal Jain1, Meredith L. Eddy1, Mady Hornig1 and W. Ian Lipkin1* Abstract Background: Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is characterized by unexplained persistent fatigue, commonly accompanied by cognitive dysfunction, sleeping disturbances, orthostatic intolerance, fever, lymphadenopathy, and irritable bowel syndrome (IBS). The extent to which the gastrointestinal microbiome and peripheral inflammation are associated with ME/CFS remains unclear. We pursued rigorous clinical characterization, fecal bacterial metagenomics, and plasma immune molecule analyses in 50 ME/CFS patients and 50 healthy controls frequency-matched for age, sex, race/ethnicity, geographic site, and season of sampling. Results: Topological analysis revealed associations between IBS co-morbidity, body mass index, fecal bacterial composition, and bacterial metabolic pathways but not plasma immune molecules. IBS co-morbidity was the strongest driving factor in the separation of topological networks based on bacterial profiles and metabolic pathways. Predictive selection models based on bacterial profiles supported findings from topological analyses indicating that ME/CFS subgroups, defined by IBS status, could be distinguished from control subjects with high predictive accuracy. Bacterial taxa predictive of ME/CFS patients with IBS were distinct from taxa associated with ME/CFS patients without IBS. Increased abundance of unclassified Alistipes and decreased Faecalibacterium emerged as the top biomarkers of ME/ CFS with IBS; while increased unclassified Bacteroides abundance and decreased Bacteroides vulgatus were the top biomarkers of ME/CFS without IBS.
    [Show full text]