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-4 -3 -2 -1 -∆Cq Unmatched Sensitivity sensitivity components, thisSuperMixenablessuperiorresultsoverawidedynamicrangeofinputRNA,andupto RNase Hactivityandimprovedstabilityathighertemperatures.Combinedwithprecisemixtureofreaction with reduced reversetranscriptase(RT) isanengineeredM-MLV qScriptXLT easy-to-use solutionfortwostepRT-qPCR. cDNASuperMixisanext-generationtoolforfirst-strandsynthesis,providinghighlysensitiveand qScript XLT DESCRIPTION: qScript XLTperformance-morethan2cyclesonaverage. a control,the Comparison ofcDNAsynthesisfrombraintissueusingqScriptXLTSuperMix (blue)andcompetitors. For more info visit:www.quantabio.com For more Premium PerformancewithSuperMixBenefits qScript FEATURES & BENEFITS: 0 1 2 3 4 Product Name qScript XLT cDNAqScript XLT SuperMix -500 R cDNAqScript XLT SuperMix R -100 cDNAqScript XLT SuperMix R -25 ORDER INFO awidedynamicrangeof across Superior results cycles 20freeze/thaw Stable through All oftheconvenienceasingle-tubeSupermix ACTB input RNA(10 ADAM19 qScript cDNASuperMix Competitor Competitor qScript XLTcDNASuperMi ALDOA APC ARAF ARHGDIA thancDNAsynthesiskitsutilizinganRNaseH(+)reversetranscriptase. ∆ Cq (changeincyclethreshold)isshownforeachof96genesfromapanelbrain tissue(higherisbetter).Theresultsshow ATP6V1G1 B2M BCL2 B A BRCA1 BUB3 8 CASP3 fold) CASP6 CCND1 ® CD40 CD86 cDNA SuperMix XLT CDKN1A x COL1A1 COPS8 CSNK2B CTNNB1 CTSB DFFA DNMT1 DNMT3A DUSP6 EGFR ENSA ERBB2 EZH2 FBXO32 FOXM1 GAPDH GDF11 GTF2I GUSB HK1 HMOX1 HPRT1 HSPA13 HSPA5 HUS1 Quantabio Catalog Number IER2 Gen IER3 IGF2 e IL1R1 IL1RN KRT18 oflow-abundancegenes Accurate representation detectionsensitivity Up to8-foldgreater LCK LDHA 95161-500 95161-025 95161-100 LIF LIFR MCM2 MCM6 MCM7 MMD MYH9 NME4 NONO NVL OGDH PERP PGK1 Reverse Transcription Using Quantabio’soriginalqScriptcDNASuperMixas PHLDA2 PPIA PREP PSMC3 PSMC4 100 rxns 500 rxns PTGS2 RB1 RPL32 RPLP0 RPSA RYBP SMARCB1 SNRPN the consistentandsuperior SNX4 TFRC 25 rxns TGFB1 Size TIMP1 TNFRSF10B 8-fold higher TNFSF10 TOP2B TP53 USP11 VCAM1 VIM WNT11 WNT5A ZIM2 (+ (–) ) cDNA Yield / RT-qPCR Sensitivity Reverse Transcription qScript® cDNA SuperMix Superior cDNA synthesis in a Single Step FEATURES & BENEFITS: Single tube convenience and simplicity Single pipetting step to minimize risk of error or contamination Fast 40 minute protocol Robust performance - accurate representation of low-copy genes Stable through 20 freeze/thaw cycles DESCRIPTION: qScript cDNA SuperMix is a reliable tool for first-strand cDNA synthesis, providing an easy-to-use and powerful solution for two step RT-qPCR. The single tube Supermix contains qScript, an engineered RNase H(+) mutant of M-MLV, and ribonuclease inhibitor protein, along with an optimized blend of oligo (dT) and random primers. Simply add your RNA and go! qScript cDNA SuperMix Other cDNA Kits Stabilized 1-tube SuperMixes simplify reaction assembly RT BufferMInhibitor g2+ and minimize risk of pipetting error Primer MMLV RT Buffer, dNTPs, Mg2+ Randomers + Oligo-d(T) RNase inhibitor 1x Add 4 µl SuperMix to RNA template Make cocktail on ice-bath Add primer & lysate to separate tube incubate & snap-chill on ice Incubate 1 Tube Add cocktail 1 Pipetting Step to primer/ template tube 40 Minutes (70 min for qScript XLT Supermix) qScript cDNA SuperMix (left) includes all necessary components in a single tube – just add RNA and go! Compare this to competitor options (right) that introduce numerous opportunities for error. 8x Incubate ORDER INFO is a trademark of QIAGEN Beverly, Inc. is a trademark of QIAGEN Beverly, Product Name Quantabio Catalog Number Size ® qScript cDNA SuperMix - 25 R 95048-025 25 rxns qScript qScript cDNA SuperMix - 100 R 95048-100 100 rxns qScript cDNA SuperMix - 500 R 95048-500 500 rxns Trademarks: Quantabio products are intended for molecular biology applications. The products are not intended for the diagnosis, prevention or treatment of a disease. or treatment not intended for the diagnosis, prevention are intended for molecular biology applications. The products are Quantabio products 0918 MK-SF-0001 REV 01 qScript SM XLT For more info visit: www.quantabio.com.
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