Reverse Transcription qScript® XLT cDNA SuperMix

Premium Performance with SuperMix Benefits

FEATURES & BENEFITS:

All of the convenience of a single-tube Supermix Up to 8-fold greater detection sensitivity Stable through 20 freeze/thaw cycles Accurate representation of low-abundance genes Superior results across a wide dynamic range of input RNA (108 fold)

DESCRIPTION: qScript XLT cDNA SuperMix is a next-generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy-to-use solution for two step RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase (RT) with reduced RNase H activity and improved activity and stability at higher temperatures. Combined with precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, and up to 8-fold higher sensitivity than cDNA synthesis kits utilizing an RNase H(+) reverse transcriptase.

Unmatched Sensitivity

-∆Cq (+) 4

3

2

1

0

-1

-2

-3 cDNA Yield / RT-qPCR Sensitivity -4 (–) LIF RB1 LIFR HK1 VIM LCK NVL IGF 2 IER2 IER3 APC PPIA B2M PERP PREP TP5 3 ZIM2 TFRC IL1R 1 RPSA RYBP BCL2 DFF A EGF R CTSB EZH2 PGK1 HUS1 SNX4 ARAF ENSA BUB3 ACTB IL1R N GTF2I LDHA MMD GUSB CD40 CD86 TIMP1 RPLP0 NME4 RPL32 TGFB1 USP11 HPRT1 MYH9 ERBB2 NONO OGDH PTGS2 KRT18 GDF11 TOP2B BRCA1 HSPA5 SNRPN CASP3 CASP6 DUSP6 COPS8 MCM2 MCM6 MCM7 WNT11 FOXM1 CCND1 PSMC3 PSMC4 DNMT1 ALDO A GAPDH VCAM1 HMOX1 WNT5 A HSPA13 FBXO32 COL1A 1 CTNNB1 PHLDA2 CSNK2B TNFSF10 CDKN1A DNMT3A ADAM19 ARHGDIA SMARCB1 ATP6V1G1 TNFRSF10B Gene

qScript XLT cDNA SuperMix Competitor A Competitor B qScript cDNA SuperMix

Comparison of cDNA synthesis from brain tissue using qScript XLT cDNA SuperMix (blue) and competitors. Using Quantabio’s original qScript cDNA SuperMix as a control, the ∆Cq (change in cycle threshold) is shown for each of 96 genes from a panel of brain tissue (higher is better). The results show the consistent and superior qScript XLT performance - more than 2 cycles on average.

ORDER INFO

Product Name Quantabio Catalog Number Size

qScript XLT cDNA SuperMix - 25 R 95161-025 25 rxns qScript XLT cDNA SuperMix - 100 R 95161-100 100 rxns qScript XLT cDNA SuperMix - 500 R 95161-500 500 rxns

For more info visit: www.quantabio.com Reverse Transcription qScript® cDNA SuperMix

Superior cDNA synthesis in a Single Step

FEATURES & BENEFITS:

Single tube convenience and simplicity Single pipetting step to minimize risk of error or contamination Fast 40 minute protocol Robust performance - accurate representation of low-copy genes Stable through 20 freeze/thaw cycles

DESCRIPTION: qScript cDNA SuperMix is a reliable tool for first-strand cDNA synthesis, providing an easy-to-use and powerful solution for two step RT-qPCR. The single tube Supermix contains qScript, an engineered RNase H(+) mutant of M-MLV, and ribonuclease inhibitor protein, along with an optimized blend of oligo (dT) and random primers. Simply add your RNA and go!

qScript cDNA SuperMix Other cDNA Kits

Stabilized 1-tube SuperMixes simplify reaction assembly RT BufferMInhibitor g2+ and minimize risk of pipetting error

Primer MMLV RT Buffer, dNTPs, Mg2+ Randomers + Oligo-d(T) RNase inhibitor

1x Add 4 µl SuperMix to RNA template Make cocktail on ice-bath

Add primer & lysate to separate tube incubate & snap-chill on ice Incubate

1 Tube Add cocktail 1 Pipetting Step to primer/ template tube 40 Minutes (70 min for qScript XLT Supermix)

qScript cDNA SuperMix (left) includes all necessary components in a single tube – just add RNA and go! Compare this to competitor options (right) that introduce numerous opportunities for error. 8x Incubate

ORDER INFO is a trademark of QIAGEN Beverly, Inc. is a trademark of QIAGEN Beverly, Product Name Quantabio Catalog Number Size ®

qScript cDNA SuperMix - 25 R 95048-025 25 rxns qScript qScript cDNA SuperMix - 100 R 95048-100 100 rxns qScript cDNA SuperMix - 500 R 95048-500 500 rxns Trademarks: Quantabio products are intended for molecular biology applications. The products are not intended for the diagnosis, prevention or treatment of a disease. or treatment not intended for the diagnosis, prevention are intended for molecular biology applications. The products are Quantabio products 0918 MK-SF-0001 REV 01 qScript SM XLT

For more info visit: www.quantabio.com