THOMAS JEFFERSON SHARPTON Department of [email protected] Oregon State University lab.sharpton.org 226 Nash Hall, Corvallis, OR 97331 @tjsharpton tel: (541) 737-8623 fax: (541) 737-0496 github.com/sharpton

EDUCATION 2009 University of California, Berkeley, CA Ph.D., Microbiology, Designated Emphasis in 2003 Oregon State University, Corvallis, OR B.S. (Cum Laude), Biochemistry and Biophysics.

APPOINTMENTS 2013- Assistant Professor Departments of Microbiology and Statistics, Oregon State University 2009-2013 Fellow Gladstone Institute of Cardiovascular Disease, Gladstone Institutes Mentors: Katherine Pollard & Jonathan Eisen (UC Davis) 2003-2009 Graduate Student Researcher Department of Microbiology, University of California, Berkeley Mentors: John Taylor & Sandrine Dudoit 2005 Bioinformatics Research Intern The Broad Institute Mentor: James Galagan 2002-2003 Research Assistant Department of Zoology, Oregon State University 2001 Research Assistant International Arctic Research Center, University of Alaska, Fairbanks, AK

HONORS & AWARDS 2013 Gladstone Scientific Leadership Award 2007 Dean’s Council Student Research Representative 2007 Effectiveness in Teaching Award, UC Berkeley 2006-2008 Chang-Lin Tien Graduate Research Fellowship 2002 Student Leadership Fellowship 2002 HHMI Undergraduate Research Fellowship 2002-2003 Dr. Donald MacDonald Excellence in Science Scholarship 2002 OSU College of Science's Excellence in Science Scholarship 1999-2003 Provost Scholarship 1999 American Legion Student Ethics Award PUBLICATIONS Refereed Journal Articles 17. O’Dwyer JP, Kembel SW, Sharpton TJ (In Review) Backbones of Evolutionary History Test Biodiversity Theory for Microbes 16. Quandt AC, Kohler A, Hesse C, Sharpton TJ, Martin F, Spatafora JW. (In Print) Metagenomic sequence of Elaphomyces granulatus from sporocarp tissue reveals Ascomycota ectomycorrhizal fingerprints of genome expansion and a Proteobacteria rich microbiome. Environmental Microbiology. 15. Skewes-Cox P, Sharpton TJ, Pollard KS, DeRisi JL. (2014) Profile hidden Markov models for the detection of viruses within metagenomic sequence data. PLOS ONE 9(8): e105067. 14. Kent M, Soderlund K, Thomann E, Schreck CB, Sharpton TJ. (2014) Post-mortem sporulation of Ceratomyxa Shasta (Myxozoa) after dealth in adult Chinook salmon. Journal of Parasitology. Published online April 11, 2014. [PMCID – In progress]. 13. Sharpton TJ^. (2014) An Introduction to the Analysis of Shotgun Metagenomic Data Frontiers in Plant Genetics and . 5(209) PMCID: PMC4059276. 12. Kidd JM*, Sharpton TJ*, Norman PJ, Carpenter M, Sikora M, Gignoux CR, Gorgani NN, Agilent Technologies, Pollard KS, Parham P, Feldman MW, Wall J, Bustamante CD, Henn BM. (2014) Exome Capture from Saliva Produces High Quality Genomic and Metagenomic Data. BMC Genomics. 15(262). PMCID: PMC4051168 11. Finucane M, Sharpton TJ, Laurant T, Pollard KS (2014) A taxonomic signature of obesity in the microbiome? Getting to the guts of the matter PLOS ONE. 9(1): e84689. PMCID: PMC3885756 10. Ladau J, Sharpton TJ, Jospin G, Kembel SW, O'Dwyer JP, Koeppel A, Green JL, Pollard KS. (2013) Global marine bacterial diversity peaks at high latitudes in winter. International Society of Journal. Published online March 21, 2013; DOI: 10.1038/ismej.2013.37. PMCID: PMC3749493 9. Sharpton TJ*^, Jospin G*, Wu D, Langille MG, Pollard KS, Eisen JA (2012). Sifting through genomes with iterative-sequence clustering produces a large, phylogenetically diverse protein-family resource, BMC Bioinformatics, 13(1): 264. PMCID: PMC3481395 8. Whiston E, Wise HZ, Jui G, Sharpton TJ, Cole GT, Taylor JW. (2012) “Comparative Transcriptomics of the Saprobic and Parasitic Growth Phases in Coccidioides spp.” PLOS ONE. 7(7): e41034. PMCID: PMC3401177 7. Wylie KM, Truty RM, Sharpton TJ, Mihindukulasuriya KA, Zhou Y, Gao H, Sodergren E, Weinstock GM, Pollard KS (2012). Novel bacterial taxa in the human microbiome, PLOS ONE, 7(6): e3529. PMCID: PMC3374617 6. The Human Microbiome Project Consortium (2012) A Framework for Human Microbiome Research, Nature, 486: 215–221. PMCID: PMC3377744 5. The Human Microbiome Project Consortium (2012). Structure, function and diversity of the human microbiome in an adult reference population, Nature, 486: 207-214. PMCID: PMC3564958 4. Sharpton TJ^, Riesenfeld SJ, Kembel SW, Ladau J, O'Dwyer JP, Green JL, Eisen JA, Pollard KS (2011). PhylOTU: a high-throughput procedure quantifies microbial community diversity and resolves novel taxa from metagenomic data, PLOS Computational Biology, 7(1): e1001061. PMCID: PMC3024254 3. Neafsey DE, Barker BM, Sharpton TJ, Stajich JE, Park D, Whiston E, Hung C, McMahan C, White J, Sykes S, Heiman D, Young S, Zeng Q, Abouelleil A, Aftuck L, Bessette D, Brown A, FitzGerald M, Lui A, Macdonald JP, Priest M, Orbach MJ, Galgiani JN, Kirkland TN, Cole GT, Birren BW, Henn MR, Taylor JW, Rounsley SD. (2010) Population Genomic Sequencing of Coccidioides Fungi Reveals Recent Hybridation and Transposon Control, Genome Research, 20: 938–946. PMCID: PMC2892095 2. Sharpton TJ^, Stajich JE, Rounsley SD, Gardner MJ, Wortman JR, Jordar VS, Maiti R, Kodira CD, Neafsey DE, Zeng Q, Hung C, McMahan C, Muszewksa A, Grynberg M, Mandel MA, Kellner EM, Barker BM, Galgiani JN, Orbach MJ, Kirkland TN, Cole GT, Henn MR, Birren BW, Taylor JW. (2009) Comparative Genomic Analyses of the Human Fungal Pathogens Coccidioides and their Relatives, Genome Research, 19: 1722– 1731. PMCID: PMC2765278 1. Sharpton TJ^, Neafsey DE, Galagan JE, and Taylor JW. (2008) Mechanisms of Intron Gain and Loss in Cryptococcus, Genome Biology, 9(1): R24. PMCID: PMC2395259 *Indicates co-first author ^Indicates corresponding author

Other Publications Sharpton TJ, Jhaveri AA. (2006) Leveraging the Knowledge of Our Peers: Online Communities Hold the Promise to Enhace Scientific Research. PLOS Biology, 4(6): e199.

PROFESSIONAL MEMBERSHIP & SERVICE

ACADEMIC SERVICE 2014- Committee Member. Microbiology Graduate Admissions, OSU 2014- Committee Member. (Under)graduate Bioinformatics Curriculum Task Force, OSU 2014- Subcommittee Co-Chair. Graduate Bioinformatics Curriculum Task Force, OSU 2014- Review Editor. Frontiers in Systems Microbiology 2013-2014 Committee Member. Center for Genome Research and Biocomputing Spring Colloquium Steering Committee, OSU 2013- Biological Consultant. Informal advisor for statisticians, OSU 2013- Statistical Consultant. Informal advisor for microbiologists, OSU 2010- Member. The Human Microbiome Project Consortium 2009- Journal Referee. Science, PLoS Computational Biology, BMC Genomics, Molecular Ecology, FEMS Microbial Ecology, Microbiome, Bioinformatics, PLoS ONE 2010 Advisor. UCSF Biomedical Informatics Graduate Program Review 2007 President. Plant and Microbial Graduate Group, UC Berkeley 2007 President. Microbiology Student Group, UC Berkeley 2006 Chair. Microbiology Student Symposium, UC Berkeley 2006-2008 Webmaster. Microbiology Student Group, UC Berkeley 2005 Reviewer. Joint Genome Institute’s IUMS Genome Browser Review

TEACHING AND TRAINING Academic Courses 2014 Introduction to Quantitative Genomics. ST599 Course Developer and Instructor (Fall Quarter) 2014 Molecular Host-Microbe Interactions. MCB637 Guest Lecture – “The Human Gut Microbiome” (5/30) 2014 Microbial Bioinformatics and Genome . OSU MB668 Course Developer and Lead Instructor. (Spring Quarter) 2014 Neuroendrocrine Regulation of Energy Metabolism. OSU NUTR 599/699 Guest Lecture – “The Human Gut Microbiome” (4/14) 2014 Nucleic Acid Bioinformatics. OSU BB499/599 CS419/519 Guest Lecture – “Inferring functional elements from conservation” (2/17) 2013 Microbial Systems. OSU MB511 Guest Lecture - “Mapping the Diversity of Marine Bacteria” (11/25) 2013 Microbiomes and Host-Microbiome Interactions. OSU HC407 Guest Lecture - “The Human Gut Microbiome” (11/12) 2007 Comparative Genomics Seminar UC Berkeley PMB290 Course Organizer 2006 The Diversity of Fungi. UC Berkeley PMB110 Graduate Student Assistant Instructor 2004 Introduction to Biology. UC Berkeley BIO1B Graduate Student Assistant Instructor 2003 Cell and Molecular Biology. OSU BI314 Undergraduate Student Assistant Instructor

ADVISING 2014- Melissa Conley, PhD Co-Advisor, Nutrition 2014 Martin Guyer. MS Co-Advisor, Statistics 2014- Nicole Kirchoff, PhD Advisor, Microbiology 2014- Christopher Gaulke, Postdoctoral Fellow, Microbiology 2014- Kyle Duyck, Undergraduate Researcher, Biochemistry/Biophysics 2014- Holman Jue, Undergraduate Researcher, Microbiology 2014- Sean McNall, Undergraduate Researcher, Microbiology 2014 Courtney Armour, Rotation Student, MCB

MENTORSHIP: INFORMAL 2013 Dr. Mariel Finucane – The obesity-associated gut microbiome 2013 Timothy Laurent – Ascertaining protein family quality and function 2012 Rachel Radnor – Computational analysis of marine microbial diversity 2012 Guillaume Jospin – Iterative clustering to identify novel proteins 2012 Stephen Nayfach – Assessing microbial functional diversity estimations 2011 Peter Skews-Cox – Building Viral HMMs for metagenomic data analysis 2011 Aram Avila-Herrera – Identification of CRISPRs in metagenomic data 2010 Dr. Rebecca Truty – Quantifying novel human microbiota

THESIS/DISSERTATION COMMITTEE MEMBER AND SERVICE 2014- Trevor Tivey, Dept. Integrative Biology, PhD Committee Member 2014- Kimberly Kenny, Honors Committee Member 2014 Krista Soderlund, Honors Thesis Committee Member 2014 Martin Guyer, Dept. Statistics, MS Committee Member 2014- Edward Davis, Dept. Botany & Plant Pathology, PhD Committee Member 2014- Kevin Vergin, Dept. Microbiology, PhD Committee Member 2013- Melissa Conley. Dept. Nutrition, PhD Committee Member 2013-2014 Robbie Eberhard-Garah. BRR Honors Student Committee Member

OPEN SOURCE SOFTWARE vFams (http://derisilab.ucsf.edu/software/price/vFam) Protein family database for the metagenomic identification of viruses Shotmap (https://github.com/sharpton/shotmap) Distributed computing workflow to classify metagenomic data into protein families SFams Database (http://edhar.genomecenter.ucdavis.edu/sifting_families/) Diverse protein family database that rapidly incorporates new genomic data PhylOTU (https://github.com/sharpton/PhylOTU) Identifies metagenomic OTUs using a phylogenetic approach ModAlign (https://github.com/sharpton/modalign) An HMM-based multiple sequence aligner Bio::SearchIO::HMMER3 (https://github.com/kblin/bioperl-hmmer3) BioPerl module that transforms HMMER result files into a BioPerl SearchIO object

GRANTS Active NIH R21 AI108953-01A1 (Co-I) Longitudinal and functional dynamics of autoimmune gut microbiomes Contact PI: Pollard Submitted: 11/2013 Collaborators: Katherine Pollard, Shomyseh Sanjabi Requested Amount: $525,250 Subcontract Request: $49,609 Department: Microbiology College: Ag. Science Person Months: 2 Location of Project: J. David Gladstone Institutes (Subcontract to OSU) Pending Dates: 10/2014-09/2016

Completed Gordon & Betty Moore Foundation 3300 (Co-PI) The Environmental Niche Atlas: Global Mapping of Microbial Functions Contact PI: Pollard Submitted 8/2011 Collaborators: Katherine Pollard, Jonathan Eisen Total Award Amount: $1,510,227 Subcontract Amount: $25,484 Department: Microbiology College: Science Active Dates: 11/01/2013 – 10/31/2014 Person-Months Per Year Committed to Project: 2 Location of Project: J. David Gladstone Institutes (Subcontract to OSU)

Submitted W.M. Keck Foundation Research Program (Co-PI) W.M. Keck Foundation Whitepaper: Developing Next-Generation High-Throughput Culturing Technology to Identify the Mechanisms Underlying Microbial Interaction Networks Contact PI: Giovannoni Submitted: 8/2014 – Internal Review Collaborators: S. Giovannoni Department: Microbiology College: Science Requested Amount: $1,893,098 Person Months: 1 Location of Project: Oregon State University Status: Phase I application submitted Sloan Foundation Early Career Award (PI) Analyzing inter-protein relationships to infer molecular, evolutionary, and ecological functions Contact PI: Sharpton Submitted: 2/2014 Collaborators: None NIH P01 (Core Co-Leader) CAM Center for Mechanistic Evaluation of Vitamin D and Xanthohumol Contact PI: Stevens Submitted: 9/2013 Collaborators: Fred Stevens, Cathy Abney, Joe Beckman, Paul Blakemore, Gerd Bobe, Jaewoo Choi, Adrian Gombart, Arup Indra, Yuan Jiang, LeeCole Legette, Malcolm Lowry, Claudia Maier, Barbara McVica, Cristobal Miranda, , Andrey Morgun, Ralph Reed, Natalia Shulzhenko, Brett Tyler Requested Amount: 9,160,816 Status: Resubmitted DOD MURI (Co-PI) Building a predictive understanding of skin bacterial community ecology, body odor, and mosquito attractiveness Contact PI: Fierer Submitted: 12/2013 Collaborators: Noah Fierer, Brendan Bohannan, Detlev Helmig, James O’Dwyer, Leslie Vosshall Requested Amount: $5,296,466 Subcontract Request: $290,621 Status: Rejected NIH R01 (Co-I) Gut microbiota mediate the interplay between immunity and glucose metabolism Contact PI: Shulzhenko Submitted: 2/2014 Collaborators: Natalia Shulzhenko, Andrey Morgun, Aleksandra Sikora Department: Microbiology College: Science Requested Amount: $1,825,000 Subaward Amount: $144,905 Department: Microbiology Person Months: 1 Location of Project: Oregon State University Pending Dates: 09/01/2014-08/31/2019 Status: Resubmitted NSF DOB (Co-PI) National Science Foundation The Elaphomyces microbiome – a model for the ecological evolution of fungal-bacterial interactions Contact PI: Spatafora Submitted: 4/2014 Collaborators: Joey Spatafora, Mike Castellano, Jeff Chang, Francis Martin Department: Microbiology College: Science Requested Amount: $2,000,000 Subcontract Amount: $500,000 Person Months: 0.5 Location of Project: Oregon State University Pending Dates: 10/2014-9/2019 Status: Rejected

Preproposals/Whitepapers Gordon and Betty Moore Foundation DDD (PI) Analyzing inter-protein relationships to infer molecular, evolutionary, and ecological functions Contact PI: Sharpton Submitted: 2/2014 Collaborators: None USDA AFRI (Co-PI) Diet and microbiome interactions during age-related inflammation Contact PI: Ho Submitted: 2/2014 Collaborators: Emily Ho, Kathy Magnusson NSF DEB – Systematics and Biodiversity (Co-PI) A metagenomic and culture-based analysis of bacterial diversity in the gut microbiome of a model marine mollusk Contact PI: King Submitted: 1/2014 Collaborators: Gary King, Cameron Thrash NSF DEB – Evolutionary Processes (Co-PI) Mechanisms that produce pan-genome diversity in globally abundant bacteria Contact PI: Giovannoni Submitted: 1/2014 Collaborators: Stephen Giovannoni US ARMY ONRBAA13-022 (Co-PI) Modes of Evolution in Genome Streamlining: The Role of Enzyme Promiscuity in Microbial Survival Contact PI: Giovannoni Submitted: 10/2013 Collaborators: Stephen Giovannoni, Patrick Deleenheer, Trent Northen

All amounts listed are total costs PRESENTATIONS INVITED TALKS 11/2014 Metagenomic Investigations of the Human Microbiome Biology Department Seminar Series, Willamette University, Salem, OR 10/2014 High-Throughput Prediction of Microbiome Physiology International Symposium on Gut Microbiology, Nanjing, China 5/2014 Data Mining the Microbiome Department of Statistics Seminar. Oregon State University, Corvallis, OR 5/2014 Metagenomic Investigations of the Human Microbiome Department of Nutrition Seminar. Oregon State University, Corvallis, OR 5/2014 From Noise to Signal: Mining Human Exome Sequences Reveals the Hunter-Gatherer Oral Microbiome Center for Genome Research and Biocomputing Spring Colloquium. Oregon State University, Corvallis, OR. 12/2013 Metagenomic Investigations of the Microbiome META Center Seminar. University of Oregon, Eugene, OR. 10/2012 Understanding the Human Microbiome through Metagenomic Analysis CGRB Seminar Series, Oregon State University, Corvallis, OR. 6/2012 Identifying novel bacteria in the human microbiome American Society of Microbiology General Meeting. San Francisco, CA. 5/2012 Characterizing oral microbiome diversity using exome captured sequences from saliva Stanford Symposium on the Biology of Hunter-Gatherers. Palo Alto, CA. 5/2012 Your Microbiome: What is it and why does it matter? Gladstone Institutes Science for Administration. San Francisco, CA. 10/2011 Mining the Metagenome: Computational approaches quantify the diversity of microbial communities San Francisco State University Dept. of Biology. San Francisco, CA. 12/2010 Characterizing microbial diversity from metagenomic data UCSF Evolution Club. San Francisco, CA. 11/2010 Characterizing microbial diversity from metagenomic data UC Berkeley Division of Biostatistics Seminar. Berkeley, CA. 6/2010 Characterizing microbial diversity from metagenomic data Banfield Lab Meeting. UC Berkeley, Berkeley, CA. 3/2009 Evolutionary genomics identifies proteins important to the interaction between Coccidioides and its host Fungal Genetics Conference. Asilomar, CA. 6/2008 The comparative genomics of Coccidioides and the rise of virulence American Society for Microbiology General Meeting. Boston MA. 11/2007 The comparative genomics of Coccidioides. UCB College of Life Sciences Dean’s Advisory Meeting. Berkeley, CA. 4/2007 What makes a pathogenic fungus? A comparative genomics investigation of Coccidioides evolution. UC Berkeley Microbiology Student Symposium. Berkeley, CA. 5/2002 The effect of light intensity on Sym 32 expression in Anthopleura elegantissima. HHMI Student Symposium. Corvallis, OR.

SOLICITED 9/2014 High-throughput annotation of metagenomes reveals community physiological variation in the mammalian microbiome (Poster) Lake Arrowhead Microbial Genomics Meeting, Lake Arrowhead, CA 8/2014 High-throughput annotation of metagenomes reveals community physiological variation in the mammalian microbiome (Talk) META Center Symposium on Host-Microbe Systems Biology, Eugene, OR 5/2012 Characterizing the diversity of the human microbiome to understand disease (Poster) Gladstone Institutes Scientific Retreat. San Francisco, CA. 3/2012 Quantifying human microbiome functional variation using Operational Protein Families (Poster) International Human Microbiome Consortium. Paris, France. 2/2012 Characterizing the diversity of the human microbiome to understand disease (Poster) Gladstone Scientific Advisory Board Meeting. San Francisco, CA. 10/2011 Novel methods to quantify human microbiome diversity (Talk) Mission Bay RIPS. UCSF, San Francisco, CA. 8/2010 PhylOTU: A high-throughput procedure that identifies Operational Taxonomic Units from metagenomic data (Poster) International Society for Microbial Ecology Annual Meeting. Seattle, WA. 6/2010 Resolving the Hidden Biosphere: A high-throughput procedure that identifies OTUs from metagenomic data (Talk) Evolution. Portland, OR. 5/2010 Characterizing microbial diversity from metagenomic data (Talk) GICD Research in Progress Seminar Series. San Francisco, CA. 4/2010 Using to Study Uncultured Microbes: Who is out there, where do they live, and what are they doing? (Poster) Gladstone Institutes Annual Retreat. San Francisco, CA. 11/2008 Comparative evolutionary genomics of the pathogenic fungus Coccidioides (Talk) UC Berkeley DE Computational and Genomic Biology Retreat. Berkeley, CA. 3/2008 The comparative genomics of Neurospora (Talk) Neurospora. Asilomar, CA. 3/2007 What makes a pathogenic fungus? (Poster) Bay Area Meeting on Pathogenesis. University of California, San Francisco, CA. 3/2007 What makes a pathogenic fungus? (Poster) Fungal Genetics Meeting. Asilomar, CA. 3/2006 The evolution of spliceosomal introns in Cryptococcus neoformans (Poster) Bay Area Meeting on Pathogenesis. University of California, San Francisco, CA