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Thogens in European Seabass and Gilthead Seabream Aquaculture

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Thogens in European Seabass and Gilthead Seabream Aquaculture Diagnostic Manual for the main pa- thogens in European seabass and Gilthead seabream aquaculture ^ Edited^ by: Snjezana Zrncic´ méditerranéennes OPTIONS OPTIONS méditerranéennes SERIES B: Studies and Research 2020 - Number 75 CIHEAM Centre International de Hautes Etudes Agronomiques Méditerranéennes International Centre for Advanced Mediterranean Agronomic Studies Président / President: Mohammed SADIKI Secretariat General / General Secretariat: Plácido PLAZA 11, rue Newton 75116 Paris, France Tél.: +33 (0) 1 53 23 91 00 - Fax: +33 (0) 1 53 23 91 01 et 02 [email protected] www.ciheam.org Le Centre International de Hautes Etudes Agronomiques Founded in 1962 at the joint initiative of the OECD and the Méditerranéennes (CIHEAM) a été créé, à l'initiative conjointe de Council of Europe, the International Centre for Advanced l'OCDE et du Conseil de l'Europe, le 21 mai 1962. C'est une Mediterranean Agronomic Studies (CIHEAM) is an organisation intergouvernementale qui réunit aujourd'hui treize intergovernmental organisation comprising thirteen member Etats membres du bassin méditerranéen (Albanie, Algérie, Egypte, countries from the Mediterranean Basin (Albania, Algeria, Egypt, Espagne, France, Grèce, Italie, Liban, Malte, Maroc, Portugal, Spain, France, Greece, Italy, Lebanon, Malta, Morocco, Portugal, Tunisie et Turquie). Tunisia and Turkey). Le CIHEAM se structure autour d'un Secrétariat général situé à CIHEAM is made up of a General Secretariat based in Paris and Paris et de quatre Instituts Agronomiques Méditerranéens (IAM), four Mediterranean Agronomic Institutes (MAI) located in Bari localisés à Bari (Italie), Chania (Grèce), Montpellier (France) et (Italy), Chania (Greece), Montpellier (France) and Zaragoza Saragosse (Espagne). (Spain). Avec au cœur de son action trois missions fondamentales In pursuing its three central missions (education, research and (formation, recherche, coopération), le CIHEAM s'est cooperation) CIHEAM has established itself as a reference in its progressivement imposé comme une référence dans ses fields of activity: Mediterranean agriculture, food and sustainable domaines d'activité : l'agriculture, l'alimentation et le rural development. développement rural durable en Méditerranée. IAM Instituts Agronomiques Méditerranéens Mediterranean Agronomic Institutes Bari - Chania - Montpellier - Zaragoza IAM-Bari IAM-Chania Dir.: Maurizio RAELI Dir.: George BAOURAKIS Via Ceglie 9 P.O. Box 85 70010 Valenzano, Bari, Italy 73100 Chania, Crete, Greece Tel. (+39) (080) 4606 111 - Fax: (+39) (080) 4606 206 Tel. (+30) 28210 35000 - Fax: (+30) 28210 35001 [email protected] [email protected] www.iamb.it www.maich.gr IAM-Montpellier IAM-Zaragoza Dir.: Pascal BERGERET Dir.: Javier SIERRA 3191, Route de Mende Avda. Montañana 1005 34093 Montpellier Cedex 5, France 50059 Zaragoza, Spain Tel. (+33) (0)4 67 04 60 00 Fax: (+33) (0)4 67 54 25 27 Tel. (+34) 976 716000 - Fax (+34) 976 716001 [email protected] et/and [email protected] [email protected] www.iamm.fr www.iamz.ciheam.org 1 Les opinions, les données et les faits exposés dans ce numéro sont sous la responsabilité des auteurs et n'engagent ni le CIHEAM, ni les Pays membres. Opinions, data and information presented in this edition are the sole responsibility of the author(s) and neither CIHEAM nor the Member Countries accept any liability therefore. Diagnostic Manual for the main pathogens in European seabass and Gilthead seabream aquaculture v v Editor: Snjezana Zrncic´ This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 727315. OPTIONS méditerranéennes Head of publication: Plácido Plaza 2020 Series B: Studies and Research Number 75 Centre International de Hautes Etudes Agronomiques Méditerranéennes International Centre for Advanced Mediterranean Agronomic Studies L’édition technique, la maquette et la mise en page de ce numéro d’Options Méditerranéennes ont été réalisées par l’Atelier d’Édition de l’IAM de Zaragoza (CIHEAM) Technical editing, layout and formatting of this edition of Options Méditerranéennes was carried out by the Editorial Board of MAI Zaragoza (CIHEAM) Crédits des photos de couverture / Cover photo credits: Tobia Pretto (Istituto Zooprofilattico Sperimentale delle Venezie) Tirage / Copy number: 400 ex. Printer: INO Reproducciones, S.A. Pol. Malpica, calle E, 32-39 (INBISA II, Nave 35) 50016 Zaragoza-Spain Dep. Legal: Z-2893-91 Fiche bibliographique / Cataloguing data: Diagnostic Manual for the main pathogens in European seabass and v Gilthead seabream aquaculture. S. Zrncic´ (ed). Zaragoza: CIHEAM 2020, 174 p. (Options Méditerranéennes, Series B: Studies and Research, No. 75) Catalogue des numéros d'Options Méditerranéennes sur / Catalogue of Options Méditerranéennes issues on: www.ciheam.org/publications ISSN: 1016 -1228 – ISBN: 978-2-85352-596-1 © CIHEAM, 2020 Reproduction partielle ou totale interdite sans l’autorisation du CIHEAM Reproduction in whole or in part is not permitted without the consent of CIHEAM List of contents Foreword ............................................................................................................................................................ 3 Abbreviations ..................................................................................................................................................... 5 Introduction ....................................................................................................................................................... 9 Part I. Sampling procedures 1. Guidelines for on-farm sampling for targeted surveillance to certify disease freedom, diagnosis in case of mortalities and for analysis of mortalities caused by unknown aetiology – S. Tavornapanich, E. Brun and M. Dverdal Jansen ....................................................................................................................... 15 2. Packing and shipping of materials for laboratory analysis with guide to shipping biological v materials – A. Toffan and S. Zrncic´ .............................................................................................................. 21 3. Guidelines for the laboratory receiving the samples – N. Cherif, S. Zaafran and K. Maatoug .............. 27 Part II. General requirements for the laboratory methods 4. Laboratory requirements for viral diseases – A. Toffan .......................................................................... 35 5. Laboratory requirements for bacterial diseases – A. Le Breton ............................................................. 39 Part III. Viral diseases with impact on Mediterranean fish farming 6. Viral Encephalopathy and Retinopathy/Viral Nervous Necrosis (VER/VNN) – A. Toffan and V. Panzarin .................................................................................................................................................... 45 Part IV. Bacterial diseases with impact on sea bass and sea bream farming v v 7. Introduction to bacterial diseases – S. Zrncic´ and ŽZ. Pavlinec................................................................ 63 8. Vibrio anguillarum – A. Manfrin ................................................................................................................. 67 9. Vibrio harveyi group – T. Pretto ................................................................................................................. 75 Options Méditerranéennes, B, no. 75, 2020 Diagnostic Manual for the main pathogens in European seabass 1 and Gilthead seabream aquaculture 10. Photobacterium damsela subsp. piscicida – P. Varvarigos ................................................................... 83 11. Tenacibaculum group – A. Le Breton ...................................................................................................... 97 12. Aeromonas spp. – M. Smyrli and P. Katharios.. ..................................................................................... 107 13. Mycobacterium group – A. Le Breton .................................................................................................. 117 Part V. Mortality caused by unknown aetiology 14. Diagnostic procedure in the case of mortality caused by unknown aetiology – N. Vendramin and S. Barsøe .................................................................................................................................................. 127 Part VI. Interpretation and reporting of results 15. Interpretation of diagnostic results in aquatic animal health – I. de Blas, A. Muniesa and I. Ruiz- Zarzuela ................................................................................................................................................... 137 v 16. Reporting of results – S. Zrncic´ and D. Oraic´................................ ........................................................ 143 Part VII. Annexes Annex 1. Summary sheets of main diseases ............................................................................................. 147 Summary sheet for viral nervous necrosis – A. Toffan ........................................................................... 148 v Summary sheet for vibriosis caused by Vibrio anguillarum – S. Zrncic´ ............................................ 150 Summary sheet for vibriosis caused by Vibrio harveyi – T. Pretto ................................ ...................... 152 Summary sheet for photobacteriosis – P. Varvarigos .............................................................................
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