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The Kinesin Superfamily Protein KIF17: One Protein with Many Functions

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The Kinesin Superfamily Protein KIF17: One Protein with Many Functions BioMol Concepts, Vol. 3 (2012), pp. 267–282 • Copyright © by Walter de Gruyter • Berlin • Boston. DOI 10.1515/bmc-2011-0064 Review The kinesin superfamily protein KIF17: one protein with many functions Margaret T.T. Wong-Riley * and Joseph C. Besharse Introduction: kinesin superfamily proteins Department of Cell Biology , Neurobiology and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank The kinesin superfamily proteins (KIFs) are microtubule- Road, Milwaukee, WI 53226 , USA based molecular motors that convert the chemical energy of adenosine triphosphate (ATP) hydrolysis to the mechani- * Corresponding author cal force of transporting cargos along microtubules. These e-mail: [email protected] ATPases were fi rst identifi ed and partially purifi ed from squid giant axons and optic lobes, the bovine brain, the chick brain, Abstract and sea urchin eggs (1 – 3) . They form a high affi nity complex with microtubules in the presence of a non-hydrolyzable ATP Kinesins are ATP-dependent molecular motors that carry analog and their kinetic property prompted the term ‘ kine- cargos along microtubules, generally in an anterograde sin ’ (1) as the counterpart to the other microtubule-associated direction. They are classified into 14 distinct families with ATPase, dynein, described two decades earlier (4) . More than varying structural and functional characteristics. KIF17 is 98 % of all KIFs (611 out of 623 KIF proteins) have been clas- a member of the kinesin-2 family that is plus end-directed. sifi ed into 14 distinct families (kinesin-1 to -14) with many It is a homodimer with a pair of head motor domains that subfamily groupings (5, 6) . Their similarity resides princi- bind microtubules, a coiled-coil stalk, and a tail domain pally in their conserved microtubule and ATP binding head that binds cargos. In neurons, KIF17 transports N -methyl- domain, while their diversity in other structural features is D-aspartate receptor NR2B subunit, kainate receptor associated with diverse functions, such as intracellular trans- GluR5, and potassium Kv4.2 channels from cell bodies port of vesicles, organelles, protein complexes, and mRNAs. exclusively to dendrites. These cargos are necessary for KIFs also contribute to spindle and chromosomal stabiliza- synaptic transmission, learning, memory and other func- tion and movement during mitosis and meiosis, engage in tions. KIF17 ’ s interaction with nuclear RNS export factor neuronal function and survival, and help in the regulation of 2 (NXF2) enables the transport of mRNA bidirectionally microtubule dynamics and developmental patterning (6 – 9) . in dendrites. KIF17 or its homolog osmotic avoidance Unlike dynein, which moves cargos toward the minus-ends of abnormal protein 3 (OSM-3) also mediates intraflagellar microtubules (10) , almost all kinesins (with the exception of transport of cargos to the distal tips of flagella or cilia, KIF2a, kinesin-14, and perhaps kinesin-5 Cin8 when acting thereby aiding in ciliogenesis. In many invertebrate and alone) move cargo toward the plus-ends of microtubules (6, vertebrate sensory cells, KIF17 delivers cargos that con- 11) . In neurons, kinesins mediate primarily fast anterograde tribute to chemosensory perception and signal transduc- transport away from the cell body, as opposed to cytoplasmic tion. In vertebrate photoreceptors, KIF17 is necessary for dynein, which moves cargo toward the cell body (1, 12) . outer segment development and disc morphogenesis. In A typical kinesin, such as conventional kinesin 1, is com- the testis, KIF17 (KIF17b) mediates microtubule-inde- posed of two heavy chains (each approx. 124 kDa) and two light pendent delivery of an activator of cAMP-responsive ele- chains (each approx. 64 kDa) (13) . Each heavy chain consists ment modulator (ACT) from the nucleus to the cytoplasm of three parts: (a) a structurally conserved globular head motor and microtubule-dependent transport of Spatial- ε , both domain that contains a catalytic site for ATP hydrolysis and a are presumably involved in spermatogenesis. KIF17 is binding site for microtubules; (b) an α -helical coiled-coil stalk also implicated in epithelial polarity and morphogenesis, domain that permits protein-protein interactions connected to placental transport and development, and the development the head domain via a family-specifi c fl exible neck linker; and of specific brain regions. The transcriptional regulation of (c) a tail domain that binds cargos (6, 13, 14) . The light chains Kif17 has recently been found to be mediated by nuclear typically bind near the tail domain of the heavy chain (13) and respiratory factor 1 (NRF-1), which also regulates NR2B are found, at least in kinesin-1, to promote cargo transport by as well as energy metabolism in neurons. Dysfunctions of suppressing the auto-inhibitory kinesin heavy chain tail-head KIF17 are linked to a number of pathologies. and tail-microtubule interactions (15) . There are many excep- tions to these organizing principles, including monomeric Keywords: ciliary transport; kinesin-2 family; arrangement of the heavy chain (kinesin-3), the absence of microtubules; NR2B transport; transcriptional control. a coiled-coil stalk domain (kinesin-13), a neck linker that is 268 M.T.T. Wong-Riley and J.C. Besharse N-terminal to the catalytic core (kinesin-14), and the absence intrafl agellar transport in virtually all cilia and fl agella and is of a cargo binding light chain (kinesin-2) (6, 13, 14) . Members essential for ciliogenesis (25) . of the kinesin superfamily are found in protozoa, fungi, plants, An excellent review of molecular motors, especially in invertebrates and vertebrates; and at least 45 of them are neurons, appeared recently (26) . The present review focuses encoded in the genomes of mice, rats and humans (6) . The mainly on a key member of the kinesin-2 family, KIF17 and majority of KIFs have their motor domain or catalytic core its diverse functions. at the N-terminus (formerly called N-kinesins); some, such as the kinesin-13 family, have it in the middle (previously known as M-kinesin) and may exhibit microtubule depolymerizing Phylogenetic relationships of kinesin-2 family activity; whereas still others have the motor domain at the members C-terminus, as exemplifi ed by the kinesin-14 family members that are minus-end directed (6, 7, 16, 17) . Kinesin-14 Ncd also Phylogenetic analysis based on protein sequence has signifi - cantly simplifi ed our understanding of the many kinesin gene has the ability to bind microtubules via both its head and tail families as well as the relationships of the many sequences domains, thus enabling crosslinking and sliding of adjacent from many species that are included in the kinesin-2 family microtubules, such as in oocyte spindle assembly (18) . [see (6) and associated website]. As shown in Figure 1 , which A large number of kinesin-associated proteins have also is a simplifi ed kinesin 2 phylogenetic tree, the kinesin heavy been identifi ed. For example, the Miro/Milton is an adaptor chains in this family fall into two distinct classes. KIF17 and protein complex that assists kinesin-1 ’ s binding to its cargo, its homologues (OSM-3 and KIN5) form a class of related the mitochondrion (19) , and Miro1 links mitochondria to homodimeric motors in which two heavy chains associate KIF5 to be transported to postsynaptic sites (20) . The kinesin through coiled-coil domains to form a functional motor with superfamily-associated protein 3 (KAP3) was originally puri- a C-terminal cargo binding domain. The second class of kine- fi ed as the 115 kDa subunit from sea urchin eggs in the het- sin-2 motors is derived from closely related heavy chains that erotrimeric complex of kinesin-related protein KRP85/95/115 are referred to in vertebrates as KIF3A, KIF3B, and KIF3C (21) and later from the mouse brain as a complex of three (6, 22, 27 – 29) . As originally shown for proteins purifi ed from high molecular weight polypeptides (hence the name) that sea urchin (21) , these heavy chains or their homologues in associate with KIF3A and KIF3B of the kinesin-2 family other species, such as Danio rerio , Caenorhabditis elegans to mediate anterograde transport of membranous organelles and Chlamydomonas reinhardtii that are included in Figure (22) . This protein was fi rst sequenced in sea urchin eggs (23) 1, form heterotrimeric complexes consisting of two different and cloned as well as characterized in the mouse brain (24) . heavy chains along with a C-terminal bound, cargo binding KAP3 has splice variants with a single species in mouse tes- protein such as KAP3 (24) . The heterotrimer is often referred tis, and the tissue-specifi c composition of KAP3 may deter- to as the KIF3 complex or simply as kinesin II (22, 29, 30) . mine functional diversity of the KIF3 complex in different The kinesin-2 family has not yet been found in fungi or higher cell types (22) . Furthermore, this heteromeric kinesin-2 plants, which are species that do not produce sperm or have motor is regarded as that canonical motor driving anterograde cilia or fl agella (6) . Although there are two evolutionarily Phylogenetic tree of the kinesin 2 family KIF3A-D. rerio KIF3A-M. musculus KLP-20-C. elegans KIF3B-D. rerio KIF3B-M. musculus Heterotrimeric motors KLP-11-C. elegans (KIF3 complex, kinesin II) FLA8-C. reinhardtii FLA10/KHP1-C. reinhardtii KIF17-D. rerio KIF17-M. musculus Homodimeric motors OSM-3-C. elegans (KIF17, OSM-3, KIN5) KIN5-T. thermophila 80 70 60 50 40 30 20 10 0 Amino acid substitution per 100 residues Figure 1 Phylogenetic tree showing the relationship of kinesin 2 family heavy chains discussed in this review. The tree was constructed with the Clustal W method of multi-sequence alignment using DNASTAR software. A more complete analysis of all kinesin heavy chains including the kinesin 2 family can be seen in (6) and the associated website. The tree shows evolutionary distance mea- sured as substitutions per 100 amino acids using the full-length sequences of each heavy chain.
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